Gene Symbol: Arl3
Description: ADP-ribosylation factor-like 3
Alias: ADP-ribosylation factor-like protein 3, ADP-ribosylation-like 3
Species: mouse
Products:     Arl3

Top Publications

  1. Zhou C, Cunningham L, Marcus A, Li Y, Kahn R. Arl2 and Arl3 regulate different microtubule-dependent processes. Mol Biol Cell. 2006;17:2476-87 pubmed
    Arl2 and Arl3 are closely related members of the Arf family of regulatory GTPases that arose from a common ancestor early in eukaryotic evolution yet retain extensive structural, biochemical, and functional features...
  2. Schrick J, Vogel P, Abuin A, Hampton B, Rice D. ADP-ribosylation factor-like 3 is involved in kidney and photoreceptor development. Am J Pathol. 2006;168:1288-98 pubmed
    ADP-ribosylation factor-like 3 (Arl3) is a member of a small subfamily of G-proteins involved in membrane-associated vesicular and intracellular trafficking processes...
  3. Evans R, Schwarz N, Nagel Wolfrum K, Wolfrum U, Hardcastle A, Cheetham M. The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport between the Golgi and the primary cilium. Hum Mol Genet. 2010;19:1358-67 pubmed publisher
    ..Here, we show that the retinitis pigmentosa protein RP2, which is a GTPase activating protein (GAP) for Arl3, localizes to the ciliary apparatus, namely the basal body and the associated centriole at the base of the ..
  4. Ismail S, Chen Y, Miertzschke M, Vetter I, Koerner C, Wittinghofer A. Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119. EMBO J. 2012;31:4085-94 pubmed publisher
    ..Previously, we have shown that the closely homologous small G proteins Arl2 and Arl3 allosterically regulate prenylated cargo release from PDE?...
  5. Fansa E, Kösling S, Zent E, Wittinghofer A, Ismail S. PDE6?-mediated sorting of INPP5E into the cilium is determined by cargo-carrier affinity. Nat Commun. 2016;7:11366 pubmed publisher
    ..High-affinity cargo is exclusively released by the ciliary transport regulator Arl3, while low-affinity cargo is released by Arl3 and its non-ciliary homologue Arl2...
  6. Ismail S, Chen Y, Rusinova A, Chandra A, Bierbaum M, Gremer L, et al. Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo. Nat Chem Biol. 2011;7:942-9 pubmed publisher
    ..We demonstrate that the G proteins Arl2 and Arl3 act in a GTP-dependent manner as allosteric release factors for farnesylated cargo...
  7. Hanke Gogokhia C, Zhang H, Frederick J, Baehr W. The Function of Arf-like Proteins ARL2 and ARL3 in Photoreceptors. Adv Exp Med Biol. 2016;854:655-61 pubmed publisher
    ..In photoreceptors, ARL2 and ARL3 participate in the trafficking of lipidated membrane-associated proteins and colocalize in the inner segment with ..
  8. Hanzal Bayer M, Linari M, Wittinghofer A. Properties of the interaction of Arf-like protein 2 with PDEdelta. J Mol Biol. 2005;350:1074-82 pubmed
    ..Among all Arl proteins tested, PDEdelta only interacted with the closely related proteins Arl2 and Arl3, strongly suggesting that Arl2/3 are specific regulators of PDEdelta.
  9. Kühnel K, Veltel S, Schlichting I, Wittinghofer A. Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3. Structure. 2006;14:367-78 pubmed
    ..RP2 is a specific effector protein of Arl3. The N-terminal 34 residues and beta helix domain of RP2 are required for this interaction...

More Information


  1. Qi Y, Jiang M, Yuan Y, Bi Y, Zheng B, Guo X, et al. ADP-ribosylation factor-like 3, a manchette-associated protein, is essential for mouse spermiogenesis. Mol Hum Reprod. 2013;19:327-35 pubmed publisher
    ..Here we have selected Arl3, one of these interesting proteins, and investigated its expression and function during spermiogenesis...
  2. Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S. Arl3 and LC8 regulate dissociation of dynactin from dynein. Nat Commun. 2014;5:5295 pubmed publisher
    ..Here we report that ADP-ribosylation factor-like 3 (Arl3) and dynein light chain LC8 induce dissociation of dynactin from dynein...
  3. Ivanova A, Caspary T, Seyfried N, Duong D, West A, Liu Z, et al. Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF). J Biol Chem. 2017;292:11091-11108 pubmed publisher
    ..from assays for solution-based nucleotide binding, intrinsic and GTPase-activating protein-stimulated GTPase, and ARL3 guanine nucleotide exchange factor activities...
  4. Wright Z, Singh R, Alpino R, Goldberg A, Sokolov M, Ramamurthy V. ARL3 regulates trafficking of prenylated phototransduction proteins to the rod outer segment. Hum Mol Genet. 2016;25:2031-2044 pubmed
    The small GTPase, ADP-ribosylation factor-like 3 (ARL3), has been proposed to participate in the transport of proteins in photoreceptor cells...
  5. Lokaj M, Kösling S, Koerner C, Lange S, van Beersum S, van Reeuwijk J, et al. The Interaction of CCDC104/BARTL1 with Arl3 and Implications for Ciliary Function. Structure. 2015;23:2122-32 pubmed publisher
    ..The ciliary protein Arl3 has been shown to act as a specific release factor for myristoylated and farnesylated ciliary cargo molecules by ..
  6. Kim H, Xu H, Yao Q, Li W, Huang Q, Outeda P, et al. Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Nat Commun. 2014;5:5482 pubmed publisher
    ..Using yeast two-hybrid screening coupled with a candidate approach, we identify a Rabep1/GGA1/Arl3-dependent ciliary targeting mechanism, whereby Rabep1 couples the polycystin complex to a GGA1/Arl3-based ciliary ..
  7. Wätzlich D, Vetter I, Gotthardt K, Miertzschke M, Chen Y, Wittinghofer A, et al. The interplay between RPGR, PDE? and Arl2/3 regulate the ciliary targeting of farnesylated cargo. EMBO Rep. 2013;14:465-72 pubmed publisher
    ..and structural considerations show that RPGR can bind with high affinity to cargo-loaded PDE? and exposes the Arl2/Arl3-binding site on PDE?...
  8. Hanzal Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig R. The complex of Arl2-GTP and PDE delta: from structure to function. EMBO J. 2002;21:2095-106 pubmed
    ..We suggest PDE delta to be a specific soluble transport factor for certain prenylated proteins and Arl2-GTP a regulator of PDE delta-mediated transport. ..
  9. Hanke Gogokhia C, Wu Z, Gerstner C, Frederick J, Zhang H, Baehr W. Arf-like Protein 3 (ARL3) Regulates Protein Trafficking and Ciliogenesis in Mouse Photoreceptors. J Biol Chem. 2016;291:7142-55 pubmed publisher
    Arf-like protein 3 (ARL3) is a ubiquitous small GTPase expressed in ciliated cells of plants and animals. Germline deletion ofArl3in mice causes multiorgan ciliopathy reminiscent of Bardet-Biedl or Joubert syndromes...
  10. Veltel S, Gasper R, Eisenacher E, Wittinghofer A. The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3. Nat Struct Mol Biol. 2008;15:373-80 pubmed publisher
    ..Previously, RP2 was shown to bind the GTP form of the small G protein Arf-like 3 (Arl3), thus qualifying as an effector...
  11. Linari M, Hanzal Bayer M, Becker J. The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE delta, interacts with the Arf-like protein Arl3 in a GTP specific manner. FEBS Lett. 1999;458:55-9 pubmed
    ..the two-hybrid system, we identify a member of the Arf-like protein family of Ras-related GTP-binding proteins, Arl3, that interacts with PDE delta...
  12. Gotthardt K, Lokaj M, Koerner C, Falk N, Gießl A, Wittinghofer A. A G-protein activation cascade from Arl13B to Arl3 and implications for ciliary targeting of lipidated proteins. elife. 2015;4: pubmed publisher
    ..Active Arl3 is involved in targeting and releasing lipidated cargo proteins from their carriers PDE6δ and UNC119a/b to the ..
  13. Lai C, Gupta N, Wen X, Rangell L, Chih B, Peterson A, et al. Functional characterization of putative cilia genes by high-content analysis. Mol Biol Cell. 2011;22:1104-19 pubmed publisher
    ..include Ssa2 and mC21orf2 with ciliation defects; Ift46 with short cilia; Ptpdc1 and Iqub with elongated cilia; and Arl3, Nme7, and Ssna1 with distinct ciliary transport but not length defects...