Gene Symbol: Apex2
Description: apurinic/apyrimidinic endonuclease 2
Alias: C430040P13Rik, ape2, DNA-(apurinic or apyrimidinic site) lyase 2, AP endonuclease 2, APEX nuclease 2
Species: mouse
Products:     Apex2

Top Publications

  1. Guikema J, Linehan E, Tsuchimoto D, Nakabeppu Y, Strauss P, Stavnezer J, et al. APE1- and APE2-dependent DNA breaks in immunoglobulin class switch recombination. J Exp Med. 2007;204:3017-26 pubmed
    ..We address the roles of the two major mammalian APEs, APE1 and APE2, in CSR...
  2. Ide Y, Tsuchimoto D, Tominaga Y, Nakashima M, Watanabe T, Sakumi K, et al. Growth retardation and dyslymphopoiesis accompanied by G2/M arrest in APEX2-null mice. Blood. 2004;104:4097-103 pubmed
    b>APEX2/APE2 is a secondary mammalian apurinic/apyrimidinic endonuclease that associates with proliferating cell nuclear antigen (PCNA), and the progression of S phase of the cell cycle is accompanied by its expression...
  3. Sabouri Z, Okazaki I, Shinkura R, Begum N, Nagaoka H, Tsuchimoto D, et al. Apex2 is required for efficient somatic hypermutation but not for class switch recombination of immunoglobulin genes. Int Immunol. 2009;21:947-55 pubmed publisher
    ..Recently, the apurinic/apyrimidinic endonucleases, Apex1 and Apex2, were reported to be involved in the DNA cleavage step of CSR...
  4. Masani S, Han L, Yu K. Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination. Mol Cell Biol. 2013;33:1468-73 pubmed publisher
    ..Finally, deletion of AP endonuclease 2 (APE2) has no effect on CSR in either APE1-proficient or -deficient cells.
  5. Ide Y, Tsuchimoto D, Tominaga Y, Iwamoto Y, Nakabeppu Y. Characterization of the genomic structure and expression of the mouse Apex2 gene. Genomics. 2003;81:47-57 pubmed
    We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice...
  6. Yui N, Yoshioka H, Fujiya H, Musha H, Beppu M, Karasawa R, et al. The DNA repair enzyme apurinic/apyrimidinic endonuclease (Apex nuclease) 2 has the potential to protect against down-regulation of chondrocyte activity in osteoarthritis. Int J Mol Sci. 2014;15:14921-34 pubmed publisher
    ..Our findings suggest that Apex 2 may have the potential to prevent the catabolic stress-mediated down-regulation of chondrocyte activity in OA. ..
  7. Dan Y, Ohta Y, Tsuchimoto D, Ohno M, Ide Y, Sami M, et al. Altered gene expression profiles and higher frequency of spontaneous DNA strand breaks in APEX2-null thymus. DNA Repair (Amst). 2008;7:1437-54 pubmed publisher
    A second class II AP endonuclease, APEX2, possesses strong 3'-5' exonuclease and 3'-phosphodiesterase activities but only very weak AP-endonuclease activity...
  8. Pei D, Yang X, Liu W, Guikema J, Schrader C, Strauss P. A novel regulatory circuit in base excision repair involving AP endonuclease 1, Creb1 and DNA polymerase beta. Nucleic Acids Res. 2011;39:3156-65 pubmed publisher
    ..These results describe a novel regulatory circuit involving Apex, Creb1 and Polb and provide a mechanism for lethality of Apex loss in higher eukaryotes. ..
  9. Stavnezer J, Linehan E, Thompson M, Habboub G, Ucher A, Kadungure T, et al. Differential expression of APE1 and APE2 in germinal centers promotes error-prone repair and A:T mutations during somatic hypermutation. Proc Natl Acad Sci U S A. 2014;111:9217-22 pubmed publisher
    ..In contrast, the less efficient homolog, APE2, is highly expressed and contributes not only to the frequency of mutations, but also to the generation of ..

More Information


  1. Guikema J, Linehan E, Esa N, Tsuchimoto D, Nakabeppu Y, Woodland R, et al. Apurinic/apyrimidinic endonuclease 2 regulates the expansion of germinal centers by protecting against activation-induced cytidine deaminase-independent DNA damage in B cells. J Immunol. 2014;193:931-9 pubmed publisher
    ..b>APE2-deficient mice have smaller GCs and reduced Ab responses compared with wild-type mice...
  2. Guikema J, Gerstein R, Linehan E, Cloherty E, Evan Browning E, Tsuchimoto D, et al. Apurinic/apyrimidinic endonuclease 2 is necessary for normal B cell development and recovery of lymphoid progenitors after chemotherapeutic challenge. J Immunol. 2011;186:1943-50 pubmed publisher
    ..However, mice deficient for the base excision repair enzyme, apurinic/apyrimidinic endonuclease 2 (APE2) protein develop relatively normally, but they display defects in lymphopoiesis...
  3. Vuong B, Herrick Reynolds K, Vaidyanathan B, Pucella J, Ucher A, Donghia N, et al. A DNA break- and phosphorylation-dependent positive feedback loop promotes immunoglobulin class-switch recombination. Nat Immunol. 2013;14:1183-1189 pubmed publisher
    ..Our results identify a positive feedback loop for the amplification of DNA breaks at S regions through the phosphorylation- and ATM-dependent interaction of AID with APE1. ..