Anxa5

Summary

Gene Symbol: Anxa5
Description: annexin A5
Alias: Anx5, R74653, annexin A5, CBP-I, PAP-I, PP4, VAC-alpha, anchorin CII, annexin V, annexin-5, calphobindin I, endonexin II, lipocortin V, placental anticoagulant protein 4, placental anticoagulant protein I, thromboplastin inhibitor, vascular anticoagulant-alpha
Species: mouse
Products:     Anxa5

Top Publications

  1. Bouter A, Gounou C, Bérat R, Tan S, Gallois B, Granier T, et al. Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat Commun. 2011;2:270 pubmed publisher
    ..Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca(2+) activation, promotes ..
  2. Brachvogel B, Moch H, Pausch F, Schlötzer Schrehardt U, Hofmann C, Hallmann R, et al. Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages. Development. 2005;132:2657-68 pubmed
    The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system...
  3. Belluoccio D, Grskovic I, Niehoff A, Schlötzer Schrehardt U, Rosenbaum S, Etich J, et al. Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization. J Bone Miner Res. 2010;25:141-53 pubmed publisher
    ..These results indicate that annexins A5 and A6 may not represent the essential annexins that promote mineralization in vivo. ..
  4. Brachvogel B, Pausch F, Farlie P, Gaipl U, Etich J, Zhou Z, et al. Isolated Anxa5+/Sca-1+ perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo. Exp Cell Res. 2007;313:2730-43 pubmed
    ..Using the specifically expressed annexin A5-LacZ fusion gene (Anxa5-LacZ), it became possible to isolate perivascular cells (PVC) from mouse tissues...
  5. van den Eijnde S, van den Hoff M, Reutelingsperger C, Van Heerde W, Henfling M, Vermeij Keers C, et al. Transient expression of phosphatidylserine at cell-cell contact areas is required for myotube formation. J Cell Sci. 2001;114:3631-42 pubmed
    ..Myotube formation in vitro can be inhibited by the PS binding protein annexin V, in contrast to its mutant M1234, which lacks the ability to bind to PS...
  6. Brachvogel B, Dikschas J, Moch H, Welzel H, von der Mark K, Hofmann C, et al. Annexin A5 is not essential for skeletal development. Mol Cell Biol. 2003;23:2907-13 pubmed
    ..The intensively studied annexin A5 has been identified by its effects on blood coagulation, and subsequently, its function as a calcium-specific ..
  7. Kallio M, Chang Y, Manuel M, Alastalo T, Rallu M, Gitton Y, et al. Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice. EMBO J. 2002;21:2591-601 pubmed
    ..Hsf2(-/-) females also display hormone response defects, that can be rescued by superovulation treatment, and exhibit abnormal rates of luteinizing hormone receptor mRNAs. ..
  8. Leikina E, Melikov K, Sanyal S, Verma S, Eun B, Gebert C, et al. Extracellular annexins and dynamin are important for sequential steps in myoblast fusion. J Cell Biol. 2013;200:109-23 pubmed publisher
  9. Sandoval H, Thiagarajan P, Dasgupta S, Schumacher A, Prchal J, Chen M, et al. Essential role for Nix in autophagic maturation of erythroid cells. Nature. 2008;454:232-5 pubmed publisher
    ..Our study may also provide insights into molecular mechanisms underlying mitochondrial quality control involving mitochondrial autophagy. ..

More Information

Publications58

  1. Taylor S, Gonzalez Begne M, Dewhurst S, Chimini G, Higgins C, Melvin J, et al. Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death. J Immunol. 2008;180:300-8 pubmed
    ..The mixed apoptotic/necrotic phenotype of P2X7-stimulated cells is consistent with a potential role for this death pathway in lupus disease. ..
  2. Zhao Y, Kuge Y, Zhao S, Morita K, Inubushi M, Strauss H, et al. Comparison of 99mTc-annexin A5 with 18F-FDG for the detection of atherosclerosis in ApoE-/- mice. Eur J Nucl Med Mol Imaging. 2007;34:1747-55 pubmed
    99m)Tc-annexin A5, a marker of ongoing apoptosis, and (18)F-FDG, a marker of the increased metabolism of inflammatory cells, are supposed to be useful in the detection of metabolically active atheroma...
  3. Kawamura H, Aswad F, Minagawa M, Govindarajan S, Dennert G. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol. 2006;176:2152-60 pubmed
    ..we show that micromolar concentrations of nicotinamide adenine dinucleotide (NAD) induce a rapid increase of annexin V staining in NKT cells in vitro, a response that requires expression of P2X(7)Rs...
  4. Poschl E, Schlötzer Schrehardt U, Brachvogel B, Saito K, Ninomiya Y, Mayer U. Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development. 2004;131:1619-28 pubmed
  5. Genetos D, Wong A, Weber T, Karin N, Yellowley C. Impaired osteoblast differentiation in annexin A2- and -A5-deficient cells. PLoS ONE. 2014;9:e107482 pubmed publisher
    ..We employed shRNA to generate annexin A2 (AnxA2)- or annexin A5 (AnxA5)-knockdown pre-osteoblasts, and determined whether proliferation or osteogenic differentiation was ..
  6. Ghislat G, Aguado C, Knecht E. Annexin A5 stimulates autophagy and inhibits endocytosis. J Cell Sci. 2012;125:92-107 pubmed publisher
    ..of purified lysosomal membranes from mouse fibroblasts, a Ca(2+)-dependent phospholipid-binding protein, annexin A5, was found to increase on lysosomal membranes under starvation...
  7. Tritsch E, Mallat Y, Lefebvre F, Diguet N, Escoubet B, Blanc J, et al. An SRF/miR-1 axis regulates NCX1 and annexin A5 protein levels in the normal and failing heart. Cardiovasc Res. 2013;98:372-80 pubmed publisher
    ..factor known to regulate the NCX1 promoter and microRNA genes, on the expression of NCX1 mRNA and protein and annexin A5 (AnxA5), a Ca(2+)-binding protein interacting with NCX1 and increased during HF...
  8. Knowlton M, Chan B, Kelly G. The zebrafish band 4.1 member Mir is involved in cell movements associated with gastrulation. Dev Biol. 2003;264:407-29 pubmed
    ..analysis was performed to identify binding partners that may participate with Mir during gastrulation, and Annexin V, a calcium channel protein, was isolated...
  9. Kobayashi K, Umezawa K, Yasui M. Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway. Biol Reprod. 2011;84:248-54 pubmed publisher
    ..These results indicate that amniotic apoptosis is induced by the TNF pathway via TNFR1 expressed in the amniotic epithelial cells and that activation of macrophages may trigger amniotic apoptosis. ..
  10. Kanaya T, Hase K, Takahashi D, Fukuda S, Hoshino K, Sasaki I, et al. The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells. Nat Immunol. 2012;13:729-36 pubmed publisher
    ..Our study demonstrates that commitment to the intestinal M-cell lineage requires Spi-B as a candidate master regulator. ..
  11. Garcia T, Farmaha J, Kow S, Hofmann M. RBPJ in mouse Sertoli cells is required for proper regulation of the testis stem cell niche. Development. 2014;141:4468-78 pubmed publisher
  12. Yonezawa T, Watanabe A, Kurusu S, Kawaminami M. Gonadotropin-releasing hormone is prerequisite for the constitutive expression of pituitary annexin A5. Endocr J. 2015;62:1127-32 pubmed publisher
    b>Annexin A5 (ANXA5), a member of the structurally related family of annexin proteins, is expressed in pituitary gonadotropes. We previously reported that ANXA5 expression is stimulated by gonadotropin-releasing hormone (GnRH)...
  13. Frey B, Munoz L, Pausch F, Sieber R, Franz S, Brachvogel B, et al. The immune reaction against allogeneic necrotic cells is reduced in Annexin A5 knock out mice whose macrophages display an anti-inflammatory phenotype. J Cell Mol Med. 2009;13:1391-9 pubmed publisher
    ..b>Annexin A5 (Anx A5) preferentially binds to PS...
  14. Maeda A, Moriguchi T, Hamada M, Kusakabe M, Fujioka Y, Nakano T, et al. Transcription factor GATA-3 is essential for lens development. Dev Dyn. 2009;238:2280-91 pubmed publisher
    ..Thus, these observations suggest that GATA-3 is essential for lens cells differentiation and proper cell cycle control. ..
  15. Goodyear R, Gale J, Ranatunga K, Kros C, Richardson G. Aminoglycoside-induced phosphatidylserine externalization in sensory hair cells is regionally restricted, rapid, and reversible. J Neurosci. 2008;28:9939-52 pubmed publisher
    ..processes, including apoptosis, PS translocates to the outer leaflet and can be labeled with externally applied annexin V, a calcium-dependent PS-binding protein...
  16. Kim P, Pai S, Brigl M, Besra G, Gumperz J, Ho I. GATA-3 regulates the development and function of invariant NKT cells. J Immunol. 2006;177:6650-9 pubmed
    ..In addition, GATA-3 is also required for survival, activation, and effector functions of this unique population of T cells. Our data also reveal a previously unidentified peripheral maturation step that is GATA-3 dependent. ..
  17. de Lau W, Kujala P, Schneeberger K, Middendorp S, Li V, Barker N, et al. Peyer's patch M cells derived from Lgr5(+) stem cells require SpiB and are induced by RankL in cultured "miniguts". Mol Cell Biol. 2012;32:3639-47 pubmed publisher
    ..We conclude that RankL-induced expression of SpiB is essential for Lgr5 stem cell-derived epithelial precursors to develop into M cells. ..
  18. Li W, Febbraio M, Reddy S, Yu D, Yamamoto M, Silverstein R. CD36 participates in a signaling pathway that regulates ROS formation in murine VSMCs. J Clin Invest. 2010;120:3996-4006 pubmed publisher
    ..These data suggest that CD36-mediated downregulation of antioxidant systems in VSMCs may contribute to its prothrombotic, proinflammatory, and atherogenic effects. ..
  19. Hyde B, Liesa M, Elorza A, Qiu W, Haigh S, Richey L, et al. The mitochondrial transporter ABC-me (ABCB10), a downstream target of GATA-1, is essential for erythropoiesis in vivo. Cell Death Differ. 2012;19:1117-26 pubmed publisher
    ..Altogether, our findings demonstrate that ABC-me is essential for erythropoiesis in vivo...
  20. Munoz L, Franz S, Pausch F, Fürnrohr B, Sheriff A, Vogt B, et al. The influence on the immunomodulatory effects of dying and dead cells of Annexin V. J Leukoc Biol. 2007;81:6-14 pubmed
    ..This membrane modification ensures a swift recognition and uptake by phagocytes of the dying and dead cells. Annexin V (AxV) preferentially binds to anionic phospholipids and thereby, modulates the clearance process...
  21. Tamehiro N, Oda H, Shirai M, Suzuki H. Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint. PLoS ONE. 2015;10:e0131047 pubmed publisher
    ..Collectively, our results indicate that an excess amount of RhoH is able to initiate pre-TCR signaling in the absence of pre-TCR complexes. ..
  22. Denny P, Lord C, Hill N, Goy J, Levy E, Podolin P, et al. Mapping of the IDDM locus Idd3 to a 0.35-cM interval containing the interleukin-2 gene. Diabetes. 1997;46:695-700 pubmed
    ..These data, taken together with the immunomodulatory role of IL-2, provide circumstantial evidence in support of the hypothesis that Idd3 is an allelic variation of the Il2 gene, or a variant in strong linkage disequilibrium. ..
  23. Verma S, Leikina E, Melikov K, Gebert C, Kram V, Young M, et al. Cell-surface phosphatidylserine regulates osteoclast precursor fusion. J Biol Chem. 2018;293:254-270 pubmed publisher
  24. Hu M, Deshpande A, Schlichting N, Hinds E, Mao C, Dose M, et al. CDK6 kinase activity is required for thymocyte development. Blood. 2011;117:6120-31 pubmed publisher
    ..This role of CDK6 as a downstream mediator of Notch identifies CDK6 kinase activity as a potential therapeutic target in human lymphoid malignancies. ..
  25. Kathiresan T, Harvey M, Orchard S, Sakai Y, Sokolowski B. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics. 2009;8:1972-87 pubmed publisher
    ..The studies described herein provide insights into BK-related functions that include not only cell excitation, but also cell signaling and apoptosis, and involve proteins concerned with Ca(2+) regulation, structure, and hearing loss. ..
  26. Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, et al. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J Biol Chem. 2012;287:33179-90 pubmed
  27. Grskovic I, Kutsch A, Frie C, Groma G, Stermann J, Schlötzer Schrehardt U, et al. Depletion of annexin A5, annexin A6, and collagen X causes no gross changes in matrix vesicle-mediated mineralization, but lack of collagen X affects hematopoiesis and the Th1/Th2 response. J Bone Miner Res. 2012;27:2399-412 pubmed publisher
    Numerous biochemical studies have pointed to an essential role of annexin A5 (AnxA5), annexin A6 (AnxA6), and collagen X in matrix vesicle-mediated biomineralization during endochondral ossification and in osteoarthritis...
  28. Rodriguez Garcia M, Kozak C, Morgan R, Fernandez M. Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics. 1996;31:151-7 pubmed
    A full-length cDNA encoding mouse annexin V (ANX5) was cloned, sequenced, and utilized for chromosomal mapping...
  29. Ueki H, Mizushina T, Laoharatchatathanin T, Terashima R, Nishimura Y, Rieanrakwong D, et al. Loss of maternal annexin A5 increases the likelihood of placental platelet thrombosis and foetal loss. Sci Rep. 2012;2:827 pubmed publisher
    Antiphospholipid syndrome is associated with an increased risk of thrombosis and pregnancy loss. Annexin A5 (Anxa5) is a candidate autoantigen...
  30. Sun X, Wei B, Liu S, Guo C, Wu N, Liu Q, et al. Anxa5 mediates the in vitro malignant behaviours of murine hepatocarcinoma Hca-F cells with high lymph node metastasis potential preferentially via ERK2/p-ERK2/c-Jun/p-c-Jun(Ser73) and E-cadherin. Biomed Pharmacother. 2016;84:645-654 pubmed publisher
    b>Annexin A5 (Anxa5) is associated with the progression of some cancers, while its role and regulation mechanism in tumor lymphatic metastasis is rarely reported...
  31. Abbas H, Maccio D, Coskun S, Jackson J, Hazen A, SILLS T, et al. Mdm2 is required for survival of hematopoietic stem cells/progenitors via dampening of ROS-induced p53 activity. Cell Stem Cell. 2010;7:606-17 pubmed publisher
    ..p16 was also stabilized because of ROS, and its loss increased cell cycling and partially rescued hematopoiesis and survival. Thus, Mdm2 is required to control ROS-induced p53 levels for sustainable hematopoiesis. ..
  32. Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen I, et al. Divergent effects of hypoxia on dendritic cell functions. Blood. 2008;112:3723-34 pubmed publisher
    ..Thus, hypoxia uncouples the promotion of inflammatory and tissue repair from sentinel functions in DCs, which we suggest is a safeguard mechanism against immune reactivity to damaged tissues. ..
  33. Chepenik K, Shipman Appasamy P, Ahn N, Goldowitz D. Developmental regulation of various annexins in the embryonic palate of the mouse: dexamethasone affects expression of annexin-1. J Craniofac Genet Dev Biol. 1995;15:171-81 pubmed
    ..These data permit the suggestion that lipocortin I plays some critical, but as yet undefined, role in modulating ontogeny of the murine palate. ..
  34. Riemann H, Loser K, Beissert S, Fujita M, Schwarz A, Schwarz T, et al. IL-12 breaks dinitrothiocyanobenzene (DNTB)-mediated tolerance and converts the tolerogen DNTB into an immunogen. J Immunol. 2005;175:5866-74 pubmed
  35. van Schaick H, Smidt M, Rovescalli A, Luijten M, Van Der Kleij A, Asoh S, et al. Homeobox gene Prx3 expression in rodent brain and extraneural tissues. Proc Natl Acad Sci U S A. 1997;94:12993-8 pubmed
    ..Prx3 may have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation. ..
  36. Haddad R, Morrow A, Plass C, Held W. Restriction landmark genomic scanning of mouse liver tumors for gene amplification: overexpression of cyclin A2. Biochem Biophys Res Commun. 2000;274:188-96 pubmed
    ..Our results also indicate that our mouse model for liver tumorigenesis in mice accurately recapitulates events observed in human hepatocellular carcinoma. ..
  37. Hosoya T, Kuroha T, Moriguchi T, Cummings D, Maillard I, Lim K, et al. GATA-3 is required for early T lineage progenitor development. J Exp Med. 2009;206:2987-3000 pubmed publisher
    ..Thus, GATA-3 is required for the cell-autonomous development of the earliest characterized thymic T cell progenitors. ..
  38. Ravassa S, Bennaghmouch A, Kenis H, Lindhout T, Hackeng T, Narula J, et al. Annexin A5 down-regulates surface expression of tissue factor: a novel mechanism of regulating the membrane receptor repertoir. J Biol Chem. 2005;280:6028-35 pubmed
    ..The protein annexin A5 (anxA5) shows high affinity for PtdSer...
  39. Brachvogel B, Welzel H, Moch H, von der Mark K, Hofmann C, Poschl E. Sequential expression of annexin A5 in the vasculature and skeletal elements during mouse development. Mech Dev. 2001;109:389-93 pubmed
    Annexin A5 (annexin V, anchorin CII) represents the prototype member of the large annexin family, characterized by its ability to interact with phospholipids in a calcium-dependent manner and to form calcium-specific ion channels...
  40. Carmeliet P, Lampugnani M, Moons L, Breviario F, Compernolle V, Bono F, et al. Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell. 1999;98:147-57 pubmed
    ..Thus, VE-cadherin/ beta-catenin signaling controls endothelial survival. ..
  41. Wilson H, Francis S, Dower S, Crossman D. Secretion of intracellular IL-1 receptor antagonist (type 1) is dependent on P2X7 receptor activation. J Immunol. 2004;173:1202-8 pubmed
    ..7 macrophages and HUVECs. Annexin V assays showed that ATP stimulation resulted in a rapid phosphatidylserine (PS) exposure on the cell surface of ..
  42. Rodriguez Garcia M, Morgan R, Fernandez M, Bances P, Fernandez M. Mouse annexin V genomic organization includes an endogenous retrovirus. Biochem J. 1999;337 ( Pt 1):125-31 pubmed
    Mouse annexin V genomic clones were characterized by restriction analysis, Southern blotting and DNA sequencing...
  43. Jeong J, Park N, Kwon Y, Ye D, Moon A, Chun Y. Role of annexin A5 in cisplatin-induced toxicity in renal cells: molecular mechanism of apoptosis. J Biol Chem. 2014;289:2469-81 pubmed publisher
    b>Annexin A5 belongs to a large family of calcium-binding and phospholipid-binding proteins and may act as an endogenous regulator of various pathophysiological processes...
  44. Stöhr R, Schurgers L, van Gorp R, Jaminon A, Marx N, Reutelingsperger C. Annexin A5 reduces early plaque formation in ApoE -/- mice. PLoS ONE. 2017;12:e0190229 pubmed publisher
    b>Annexin A5 (AnxA5) exerts anti-inflammatory, anticoagulant and anti-apoptotic effects through its binding to cell surface expressed phosphatidylserine...
  45. Yan X, Doffek K, Yin C, Krein M, Phillips M, Sugg S, et al. Annexin-V promotes anti-tumor immunity and inhibits neuroblastoma growth in vivo. Cancer Immunol Immunother. 2012;61:1917-27 pubmed publisher
    ..T cells isolated from mice immunized with AnV-secreting but not control vaccine lysed AGN2a. In summary, AnV blocked PS, enhanced T cell mediated tumor immunity, and inhibited tumor growth. ..
  46. Peng B, Liu S, Guo C, Sun X, Sun M. ANXA5 level is linked to in vitro and in vivo tumor malignancy and lymphatic metastasis of murine hepatocarcinoma cell. Future Oncol. 2016;12:31-42 pubmed publisher
    To investigate ANXA5 overexpression on in vitro and in vivo malignancies of murine Hca-P cells. Hca-P with low lymph node metastasis (LNM) potential was used as cell model...
  47. Liu B, Song X, Lu X, Fang C, Wei S, Alli A, et al. Lovastatin attenuates effects of cyclosporine A on tight junctions and apoptosis in cultured cortical collecting duct principal cells. Am J Physiol Renal Physiol. 2013;305:F304-13 pubmed publisher
  48. Yoshida S, Minematsu N, Chubachi S, Nakamura H, Miyazaki M, Tsuduki K, et al. Annexin V decreases PS-mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice. Am J Physiol Lung Cell Mol Physiol. 2012;303:L852-60 pubmed publisher
    ..In this study we pharmacologically inhibited efferocytosis with annexin V and attempted to determine its impact on the progression of pulmonary emphysema in mouse...
  49. Baleriola J, Suarez T, de la Rosa E. DNA-PK promotes the survival of young neurons in the embryonic mouse retina. Cell Death Differ. 2010;17:1697-706 pubmed publisher
    ..Therefore, our results implicate the generation of DSB and DNA-PK-mediated repair in neurogenesis in the developing retina. ..