Gene Symbol: Anpep
Description: alanyl (membrane) aminopeptidase
Alias: AP-M, AP-N, Apn, Cd13, P150, aminopeptidase N, alanyl aminopeptidase, aminopeptidase M, aminopeptidase N/CD13, membrane protein p161, microsomal aminopeptidase
Species: mouse
Products:     Anpep

Top Publications

  1. Ben Zvi A, Lacoste B, Kur E, Andreone B, Mayshar Y, Yan H, et al. Mfsd2a is critical for the formation and function of the blood-brain barrier. Nature. 2014;509:507-11 pubmed publisher
    ..These findings identify Mfsd2a as a key regulator of BBB function that may act by suppressing transcytosis in CNS endothelial cells. Furthermore, our findings may aid in efforts to develop therapeutic approaches for CNS drug delivery...
  2. Ben David Y, Giddens E, Bernstein A. Identification and mapping of a common proviral integration site Fli-1 in erythroleukemia cells induced by Friend murine leukemia virus. Proc Natl Acad Sci U S A. 1990;87:1332-6 pubmed
    ..DNA and RNA analysis suggests, however, that Fli-1 is different from ets-1. Thus, Fli-1 appears to define a distinct locus specifically involved in the induction of erythroid leukemias by F-MuLV...
  3. Dondossola E, Rangel R, Guzman Rojas L, Barbu E, Hosoya H, St John L, et al. CD13-positive bone marrow-derived myeloid cells promote angiogenesis, tumor growth, and metastasis. Proc Natl Acad Sci U S A. 2013;110:20717-22 pubmed publisher
    ..We have recently demonstrated that CD13 (aminopeptidase N) expressed by nonmalignant host cells of unspecified types regulate tumor blood vessel development...
  4. Khatun A, Rahman M, Ryu D, Kwon W, Pang M. Elevated aminopeptidase N affects sperm motility and early embryo development. PLoS ONE. 2017;12:e0184294 pubmed publisher
    b>Aminopeptidase N (APN) is a naturally occurring ectopeptidase present in mammalian semen. Previous studies have demonstrated that APN adversely affects male fertility through the alteration of sperm motility. This enzyme constitutes 0...
  5. Rangel R, Sun Y, Guzman Rojas L, Ozawa M, Sun J, Giordano R, et al. Impaired angiogenesis in aminopeptidase N-null mice. Proc Natl Acad Sci U S A. 2007;104:4588-93 pubmed
    b>Aminopeptidase N (APN, CD13; EC is a transmembrane metalloprotease with several functions, depending on the cell type and tissue environment...
  6. Datta S, Owen F, Womack J, Riblet R. Analysis of recombinant inbred lines derived from "autoimmune" (NZB) and "high leukemia" (C58) strains: independent multigenic systems control B cell hyperactivity, retrovirus expression, and autoimmunity. J Immunol. 1982;129:1539-44 pubmed
  7. Sherwood R, Maehr R, Mazzoni E, Melton D. Wnt signaling specifies and patterns intestinal endoderm. Mech Dev. 2011;128:387-400 pubmed publisher
    ..These findings shed light on the mechanism of embryonic intestinal induction and provide a method to manipulate intestinal development from embryonic stem cells. ..
  8. Ghosh M, McAuliffe B, Subramani J, Basu S, Shapiro L. CD13 regulates dendritic cell cross-presentation and T cell responses by inhibiting receptor-mediated antigen uptake. J Immunol. 2012;188:5489-99 pubmed publisher
    ..In this study, we found that the myeloid cell surface peptidase CD13 is highly and specifically expressed on the subset of DCs responsible for cross-presentation, the CD8(+) murine ..
  9. Kolb A, Sorrell D, Lassnig C, Lillico S, Carlisle A, Neil C, et al. Mammary gland development is delayed in mice deficient for aminopeptidase N. Transgenic Res. 2013;22:425-34 pubmed publisher
    ..We have analysed the role of the cell surface protease aminopeptidase N (Anpep, APN, CD13) during these processes using Anpep deficient and Anpep over-expressing mice...

More Information


  1. Hildebrand C, Gonzalez F, Kozak C, Nebert D. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem Biophys Res Commun. 1985;130:396-406 pubmed
  2. Pereira F, Cronin C, Ghosh M, Zhou S, Agosto M, Subramani J, et al. CD13 is essential for inflammatory trafficking and infarct healing following permanent coronary artery occlusion in mice. Cardiovasc Res. 2013;100:74-83 pubmed publisher
    To determine the role of CD13 as an adhesion molecule in trafficking of inflammatory cells to the site of injury in vivo and its function in wound healing following myocardial infarction induced by permanent coronary artery occlusion...
  3. Subramani J, Ghosh M, Rahman M, Caromile L, Gerber C, Rezaul K, et al. Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking. J Immunol. 2013;191:3905-12 pubmed publisher
    b>CD13 is a large cell surface peptidase expressed on the monocytes and activated endothelial cells that is important for homing to and resolving the damaged tissue at sites of injury...
  4. Miller R, Ozaki J, Riblet R, Gold D. Genetic mapping of mouse T3d and T3e between Apoa1 and Ncam. Immunogenetics. 1989;30:511-4 pubmed
  5. Rahman M, Ghosh M, Subramani J, Fong G, Carlson M, Shapiro L. CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury. Stem Cells. 2014;32:1564-77 pubmed publisher
    b>CD13 is a multifunctional cell surface molecule that regulates inflammatory and angiogenic mechanisms in vitro, but its contribution to these processes in vivo or potential roles in stem cell biology remains unexplored...
  6. Ghosh M, Gerber C, Rahman M, Vernier K, Pereira F, Subramani J, et al. Molecular mechanisms regulating CD13-mediated adhesion. Immunology. 2014;142:636-47 pubmed publisher
    CD13/Aminopeptidase N is a transmembrane metalloproteinase that is expressed in many tissues where it regulates various cellular functions...
  7. Guzman Rojas L, Rangel R, Salameh A, Edwards J, Dondossola E, Kim Y, et al. Cooperative effects of aminopeptidase N (CD13) expressed by nonmalignant and cancer cells within the tumor microenvironment. Proc Natl Acad Sci U S A. 2012;109:1637-42 pubmed publisher
    ..The metalloprotease aminopeptidase N (APN; CD13) is often overexpressed in tumor cells and has been implicated in angiogenesis and cancer ..
  8. Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
    ..Our findings identify a novel and unexpected population of progenitors for coronary mural cells with potential relevance for heart function and disease conditions. ..
  9. Winnicka B, O Conor C, Schacke W, Vernier K, Grant C, Fenteany F, et al. CD13 is dispensable for normal hematopoiesis and myeloid cell functions in the mouse. J Leukoc Biol. 2010;88:347-59 pubmed publisher
    The robust and consistent expression of the CD13 cell surface marker on very early as well as differentiated myeloid hematopoietic cells has prompted numerous investigations seeking to define roles for CD13 in myeloid cells...
  10. Mina Osorio P, Winnicka B, O Conor C, Grant C, Vogel L, Rodriguez Pinto D, et al. CD13 is a novel mediator of monocytic/endothelial cell adhesion. J Leukoc Biol. 2008;84:448-59 pubmed publisher
    ..The transmembrane molecule CD13 is expressed on monocytes and endothelial cells and has been shown to mediate homotypic cell adhesion, which may ..
  11. Buehler A, van Zandvoort M, Stelt B, Hackeng T, Schrans Stassen B, Bennaghmouch A, et al. cNGR: a novel homing sequence for CD13/APN targeted molecular imaging of murine cardiac angiogenesis in vivo. Arterioscler Thromb Vasc Biol. 2006;26:2681-7 pubmed
    Previously, the peptide sequence cNGR has been shown to home specifically to CD13/APN (aminopeptidase N) on tumor endothelium...
  12. Douglas T, Dawson P. Location of the gene for theta antigen in the mouse. III. The position of Thy-1 relative to Lap-1 and Mpi-1. J Hered. 1979;70:250-4 pubmed
    ..The overall map for this portion of chromosome 9 as determined by these crosses is: Lap-1--5--Thy-1--7--Mpi-1--14--Mod-1. ..
  13. Potolicchio I, Carven G, Xu X, Stipp C, Riese R, Stern L, et al. Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. J Immunol. 2005;175:2237-43 pubmed
    ..Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1...
  14. Womack J, Lynes M, Taylor B. Genetic variation of an intestinal leucine arylaminopeptidase (Lap-1) in the mouse and its location on chromosome 9. Biochem Genet. 1975;13:511-8 pubmed
    ..The Lap-1 to d map distance was estimated to be 21.3 +/- 4.6 cM from backcross data and 8.1 +/- 4.8 cM from recombinant inbred lines. ..
  15. Larsen S, Pedersen L, Buus S, Stryhn A. T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides. J Exp Med. 1996;184:183-9 pubmed
    ..Specific antibodies further identified the exopeptidase Aminopeptidase N (APN, CD13) as one of the enzymes involved in the observed cell-surface antigen processing...
  16. Amiguet P, D EUSTACHIO P, Kristensen T, Wetsel R, Saris C, Hunter T, et al. Structure and chromosome assignment of the murine p36 (calpactin I heavy chain) gene. Biochemistry. 1990;29:1226-32 pubmed
    ..The p36 gene is at least 22 kb in length and has a coding sequence of approximately 1 kb, representing only 4.5% of the gene.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  17. Hansen A, Noren O, Sjostrom H, Werdelin O. A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules. Eur J Immunol. 1993;23:2358-64 pubmed
    To analyze the expression of mouse aminopeptidase N (APN) on the cells of the immune system a panel of rat monoclonal antibodies against mouse intestinal APN was generated...
  18. Finlay M, McCloud L. Intestinal leucine aminopeptidase and alkaline phosphatase: genetic regulation and development in mice. Biochem Genet. 1990;28:267-81 pubmed
    ..In interstrain crosses, banded/diffuse, migration rate, and neuraminidase sensitivity were inherited as independent autosomal traits, with indications of variable penetrance and genetic interaction. ..
  19. Eicher E, Taylor B, Leighton S, Womack J. A serum protein polymorphism determinant on chromosome 9 of Mus musculus. Mol Gen Genet. 1980;177:571-6 pubmed
    ..Because the genotype of SEP-1 can be determined for individual mice without killing them, Sep-1 is a very useful gene in linkage studies and experimental biology. ..
  20. Potter T, Hogarth P, McKenzie I. Ly-15: a new murine lymphocyte alloantigenic locus. Transplantation. 1981;31:339-42 pubmed
    ..1. A monoclonal antibody to the Ly-15.2 specificity is also described. The Ly-15 specificities were found on thymus, on lymph node and spleen cells, and on liver, but were absent from kidney, brain, and red blood cells. ..
  21. Youngblood G, Nesbitt M, Payne A. The structural genes encoding P450scc and P450arom are closely linked on mouse chromosome 9. Endocrinology. 1989;125:2784-6 pubmed
    ..The information presented in this report, along with other studies, indicate conservation between homologous human and mouse chromosomal regions and suggest that human P450SCC will be found to be closely linked with human P450arom. ..
  22. Iturrioz X, Rozenfeld R, Michaud A, Corvol P, Llorens Cortes C. Study of asparagine 353 in aminopeptidase A: characterization of a novel motif (GXMEN) implicated in exopeptidase specificity of monozinc aminopeptidases. Biochemistry. 2001;40:14440-8 pubmed
    ..In conclusion, Asn-353 of the GXMEN motif, together with Glu-352, contributes to the exopeptidase specificity of APA and plays an equivalent role to Asn-144 in CPA. ..
  23. Womack J, Taylor B, Barton J. Esterase 13, a new mouse esterase locus with recessive expression and its genetic location on chromosome 9. Biochem Genet. 1978;16:1107-12 pubmed
    ..Es-13 was mapped on Chr 9 by recombinant inbred lines and by conventional backcrossing experiments. Backcross data produced the following gene order and map distances: Lap-1 (31.6 +/- 7.5 cM) Es-13 (2.6 +/- 2.6 cM) Mod-1. ..
  24. Wallingford M, Gammill H, Giachelli C. Slc20a2 deficiency results in fetal growth restriction and placental calcification associated with thickened basement membranes and novel CD13 and lamininα1 expressing cells. Reprod Biol. 2016;16:13-26 pubmed publisher
    ..vascular structure, increased basement membrane deposition, abundant calcification, and accumulation of novel CD13 and lamininα1 positive cells...
  25. Zotz J, Wölbing F, Lassnig C, Kauffmann M, Schulte U, Kolb A, et al. CD13/aminopeptidase N is a negative regulator of mast cell activation. FASEB J. 2016;30:2225-35 pubmed publisher
    ..S., Wölbing, F., Lassnig, C., Kauffmann, M., Schulte, U., Kolb, A., Whitelaw, B., Müller, M., Biedermann, T., Huber, M. CD13/aminopeptidase N is a negative regulator of mast cell activation.
  26. Ramagli L, Womack J, Rodriguez L. Genetic analysis of nonhistone chromosomal protein inheritance in recombinant inbred mouse strains using two-dimensional electrophoresis. Biochem Genet. 1990;28:123-36 pubmed
    ..These 16 NHCP genetic marker inheritance differences detected in RIs add to the 23 previously established genetic marker differences between the progenitors. ..
  27. Look A, Ashmun R, Shapiro L, Peiper S. Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J Clin Invest. 1989;83:1299-307 pubmed
    ..Sequence comparisons with known enzymes of this class revealed that CD13 is identical to aminopeptidase N, a membrane-bound glycoprotein thought to be involved in the metabolism of regulatory peptides by diverse ..
  28. D EUSTACHIO P, Owens G, Edelman G, Cunningham B. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A. 1985;82:7631-5 pubmed
    ..The Ncam locus provides an important reference point for mapping the genes for additional cell adhesion molecules as well as genes for other molecules involved in neural development and function. ..
  29. Lusis A, Taylor B, Wangenstein R, LeBoeuf R. Genetic control of lipid transport in mice. II. Genes controlling structure of high density lipoproteins. J Biol Chem. 1983;258:5071-8 pubmed
    ..Thus, the mouse provides a useful model system for examining the genetic control of mammalian HDL structure and regulation. ..
  30. Antonucci T, von Deimling O, Rosenblum B, Skow L, Meisler M. Conserved linkage within a 4-cM region of mouse chromosome 9 and human chromosome 11. Genetics. 1984;107:463-75 pubmed
    ..Evidence is presented for the homology of this chromosome region with the ESA4 - UPS - APO-AI region on the long arm of human chromosome 11. ..
  31. Di Matteo P, Arrigoni G, Alberici L, Corti A, Gallo Stampino C, Traversari C, et al. Enhanced expression of CD13 in vessels of inflammatory and neoplastic tissues. J Histochem Cytochem. 2011;59:47-59 pubmed publisher
    Aminopeptidase-N (CD13) is an important target of tumor vasculature-targeting drugs...
  32. Rossdeutsch A, Smart N, Dubé K, Turner M, Riley P. Essential role for thymosin ?4 in regulating vascular smooth muscle cell development and vessel wall stability. Circ Res. 2012;111:e89-102 pubmed publisher
    ..These findings have important implications for understanding congenital anomalies that may be causative for adult-onset vascular instability. ..
  33. Fairweather S, Bröer A, O Mara M, Broer S. Intestinal peptidases form functional complexes with the neutral amino acid transporter B(0)AT1. Biochem J. 2012;446:135-48 pubmed publisher
    ..of intestinal brush-border membrane proteins revealed that B(0)AT1 forms complexes with two peptidases, APN (aminopeptidase N/CD13) and ACE2 (angiotensin-converting enzyme 2)...
  34. Doehring L, Heeger C, Aherrahrou Z, Kaczmarek P, Erdmann J, Schunkert H, et al. Myeloid CD34+CD13+ precursor cells transdifferentiate into chondrocyte-like cells in atherosclerotic intimal calcification. Am J Pathol. 2010;177:473-80 pubmed publisher
    ..stem cells contribute to the phenotype, stem cell marker CD34 and myeloid progenitor-associated antigen CD13 were analyzed. CD34(+) was detectable in 86.9 +/- 8.1% and CD13(+) evidence in 54.2 +/- 7...
  35. Ryan A, Womack J, Yu J, Lin J, Wu X, Sun T, et al. Chromosomal localization of uroplakin genes of cattle and mice. Mamm Genome. 1993;4:656-61 pubmed
    ..Our results also suggest likely positions for two human uroplakin genes and should facilitate further analysis of their possible involvement in disease. ..
  36. Ghosh M, Subramani J, Rahman M, Shapiro L. CD13 restricts TLR4 endocytic signal transduction in inflammation. J Immunol. 2015;194:4466-76 pubmed publisher
    ..We showed that CD13 negatively modulates receptor-mediated Ag uptake in dendritic cells to control T cell activation in adaptive ..
  37. Nefla M, Sudre L, Denat G, Priam S, Andre Leroux G, Berenbaum F, et al. The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage. J Cell Sci. 2015;128:3250-62 pubmed publisher
    ..The aim of this study was to investigate the involvement of CD13 (also known as aminopeptidase N, APN) in the chondrocyte response to 14-3-3ε...
  38. Moore B, Jin R, Osaki L, Romero Gallo J, Noto J, Peek R, et al. Identification of alanyl aminopeptidase (CD13) as a surface marker for isolation of mature gastric zymogenic chief cells. Am J Physiol Gastrointest Liver Physiol. 2015;309:G955-64 pubmed publisher
    ..Here, we identify alanyl aminopeptidase (CD13) as a specific surface marker of zymogenic chief cells (ZCs) in the gastric epithelium...
  39. Yang E, Shim J, Woo H, Kim K, Kwon H. Aminopeptidase N/CD13 induces angiogenesis through interaction with a pro-angiogenic protein, galectin-3. Biochem Biophys Res Commun. 2007;363:336-41 pubmed
    b>Aminopeptidase N/CD13 is an endothelial cell surface ectoenzyme involved in one of the initial steps of angiogenesis. However, little is known how APN induces angiogenesis in endothelial cells...
  40. Chen H, Kinzer C, Paul W. p161, a murine membrane protein expressed on mast cells and some macrophages, is mouse CD13/aminopeptidase N. J Immunol. 1996;157:2593-600 pubmed
    ..The sequences of the resultant peptides indicated that p161 is homologous with rat and human CD13/aminopeptidase N. Using oligonucleotide primers derived from rat CD13 cDNA, a mouse cDNA was obtained...
  41. Biton A, Bank U, Tager M, Ansorge S, Reinhold D, Lendeckel U, et al. Dipeptidyl peptidase IV (DP IV, CD26) and aminopeptidase N (APN, CD13) as regulators of T cell function and targets of immunotherapy in CNS inflammation. Adv Exp Med Biol. 2006;575:177-86 pubmed
  42. Paradis H, Liu C, Saika S, Azhar M, Doetschman T, Good W, et al. Tubedown-1 in remodeling of the developing vitreal vasculature in vivo and regulation of capillary outgrowth in vitro. Dev Biol. 2002;249:140-55 pubmed
    ..Moreover, vitreal vessels of TGF-beta2(-/-) mice lack expression of pericyte markers (CD13, alpha smooth muscle actin) and show ultrastructural changes consistent with pericyte degeneration...