Gene Symbol: Akap5
Description: A kinase (PRKA) anchor protein 5
Alias: 3526401B18Rik, AKAP 150, AKAP-5, AKAP150, BB098886, Gm258, P150, A-kinase anchor protein 5, A-kinase anchor protein 150 kDa, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Species: mouse
Products:     Akap5

Top Publications

  1. Lu Y, Allen M, Halt A, Weisenhaus M, Dallapiazza R, Hall D, et al. Age-dependent requirement of AKAP150-anchored PKA and GluR2-lacking AMPA receptors in LTP. EMBO J. 2007;26:4879-90 pubmed
    Association of PKA with the AMPA receptor GluR1 subunit via the A kinase anchor protein AKAP150 is crucial for GluR1 phosphorylation...
  2. Navedo M, Nieves Cintrón M, Amberg G, Yuan C, Votaw V, Lederer W, et al. AKAP150 is required for stuttering persistent Ca2+ sparklets and angiotensin II-induced hypertension. Circ Res. 2008;102:e1-e11 pubmed publisher
    ..We have identified AKAP150 as the key protein, which targets protein kinase Calpha to the L-type Ca2+ channels and thereby enables its ..
  3. Lu Y, Zhang M, Lim I, Hall D, Allen M, Medvedeva Y, et al. AKAP150-anchored PKA activity is important for LTD during its induction phase. J Physiol. 2008;586:4155-64 pubmed publisher
    ..PKA is recruited to postsynaptic sites by the A kinase anchor protein AKAP150. We found that LTD was strongly impaired in acute hippocampal slices from 2-week-old mice in which the PKA ..
  4. Bhattacharyya S, Biou V, Xu W, Schlüter O, Malenka R. A critical role for PSD-95/AKAP interactions in endocytosis of synaptic AMPA receptors. Nat Neurosci. 2009;12:172-81 pubmed publisher
    ..endocytosis of synaptic AMPARs in rat neuron cultures because of its binding to A kinase-anchoring protein 150 (AKAP150), a scaffold for specific protein kinases and phosphatases...
  5. Sandoz G, Thümmler S, Duprat F, Feliciangeli S, Vinh J, Escoubas P, et al. AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels. EMBO J. 2006;25:5864-72 pubmed
    ..Here, we show that the A-kinase-anchoring protein AKAP150 is a constituent of native TREK-1 channels...
  6. Hall D, Davare M, Shi M, Allen M, Weisenhaus M, McKnight G, et al. Critical role of cAMP-dependent protein kinase anchoring to the L-type calcium channel Cav1.2 via A-kinase anchor protein 150 in neurons. Biochemistry. 2007;46:1635-46 pubmed
    ..2 subunit. Here we demonstrate that the A-kinase anchor protein 150 (AKAP150) is critical for PKA-mediated regulation of Cav1.2 in the brain...
  7. Weisenhaus M, Allen M, Yang L, Lu Y, Nichols C, Su T, et al. Mutations in AKAP5 disrupt dendritic signaling complexes and lead to electrophysiological and behavioral phenotypes in mice. PLoS ONE. 2010;5:e10325 pubmed publisher
    AKAP5 (also referred to as AKAP150 in rodents and AKAP79 in humans) is a scaffolding protein that is highly expressed in neurons and targets a variety of signaling molecules to dendritic membranes...
  8. Nijholt I, Dolga A, Ostroveanu A, Luiten P, Schmidt M, Eisel U. Neuronal AKAP150 coordinates PKA and Epac-mediated PKB/Akt phosphorylation. Cell Signal. 2008;20:1715-24 pubmed publisher
    ..PKA, PKB/Akt and Epac were all shown to establish complexes with neuronal A-kinase anchoring protein150 (AKAP150). Interestingly, activation of Epac increased phosphorylation of PKB/Akt complexed to AKAP150...
  9. Tunquist B, Hoshi N, Guire E, Zhang F, Mullendorff K, Langeberg L, et al. Loss of AKAP150 perturbs distinct neuronal processes in mice. Proc Natl Acad Sci U S A. 2008;105:12557-62 pubmed publisher
    ..b>AKAP150 brings protein kinase A (PKA), the calcium/calmodulin dependent phosphatase PP2B and protein kinase C (PKC) to ..

More Information


  1. Wang D, Govindaiah G, Liu R, De Arcangelis V, Cox C, Xiang Y. Binding of amyloid beta peptide to beta2 adrenergic receptor induces PKA-dependent AMPA receptor hyperactivity. FASEB J. 2010;24:3511-21 pubmed publisher
    ..beta(2)AR and GluR1 also form a complex comprising postsynaptic density protein 95 (PSD95), PKA and its anchor AKAP150, and protein phosphotase 2A (PP2A)...
  2. Di Biase V, Obermair G, Szabo Z, Altier C, Sanguesa J, Bourinet E, et al. Stable membrane expression of postsynaptic CaV1.2 calcium channel clusters is independent of interactions with AKAP79/150 and PDZ proteins. J Neurosci. 2008;28:13845-55 pubmed publisher
    ..2 is independent of its interactions with AKAP79/150 and PDZ proteins. ..
  3. Sanderson J, Gorski J, Dell Acqua M. NMDA Receptor-Dependent LTD Requires Transient Synaptic Incorporation of Ca²⁺-Permeable AMPARs Mediated by AKAP150-Anchored PKA and Calcineurin. Neuron. 2016;89:1000-15 pubmed publisher
    ..Here, we show that PKA anchored to the scaffold protein AKAP150 regulates GluA1 phosphorylation and plays a novel role controlling CP-AMPAR synaptic incorporation during NMDAR-..
  4. Li X, Nooh M, Bahouth S. Role of AKAP79/150 protein in ?1-adrenergic receptor trafficking and signaling in mammalian cells. J Biol Chem. 2013;288:33797-812 pubmed publisher
    ..We examined the role of AKAP79/150 (AKAP5) in trafficking and signaling of the ?1-adrenergic receptor (?1-AR)...
  5. Wu C, DiJulio D, Jacobson K, McKnight G, Watson E. The contribution of AKAP5 in amylase secretion from mouse parotid acini. Am J Physiol Cell Physiol. 2010;298:C1151-8 pubmed publisher
    ..In the present study, AKAP5 (formerly AKAP150) and AKAP6 were identified in mouse parotid acini by type II PKA regulatory subunit (RII) overlay assay and ..
  6. Zhang M, Patriarchi T, Stein I, Qian H, Matt L, Nguyen M, et al. Adenylyl cyclase anchoring by a kinase anchor protein AKAP5 (AKAP79/150) is important for postsynaptic ?-adrenergic signaling. J Biol Chem. 2013;288:17918-31 pubmed publisher
    Recent evidence indicates that the A kinase anchor protein AKAP5 (AKAP79/150) interacts not only with PKA but also with various adenylyl cyclase (AC) isoforms...
  7. Cheng E, Yuan C, Navedo M, Dixon R, Nieves Cintrón M, Scott J, et al. Restoration of normal L-type Ca2+ channel function during Timothy syndrome by ablation of an anchoring protein. Circ Res. 2011;109:255-61 pubmed publisher
    ..2-LQT8 currents and generates lethal arrhythmias are unclear. To test the hypothesis that the anchoring protein AKAP150 modulates Ca(V)1.2-LQT8 channel gating in ventricular myocytes...
  8. Sanderson J, Gorski J, Gibson E, Lam P, Freund R, Chick W, et al. AKAP150-anchored calcineurin regulates synaptic plasticity by limiting synaptic incorporation of Ca2+-permeable AMPA receptors. J Neurosci. 2012;32:15036-52 pubmed publisher
    ..phosphatase calcineurin (CaN) are targeted to GluA1 through binding to A-kinase anchoring protein 150 (AKAP150) in a complex with PSD-95, but we do not understand how the opposing activities of these enzymes are balanced to ..
  9. Zhang J, Shapiro M. Activity-dependent transcriptional regulation of M-Type (Kv7) K(+) channels by AKAP79/150-mediated NFAT actions. Neuron. 2012;76:1133-46 pubmed publisher
    ..demonstrated by profoundly upregulated KCNQ2/3 transcription in hippocampi from wild-type, but not AKAP150(-/-), mice after drug-induced seizures...
  10. Nijholt I, Ostroveanu A, de Bruyn M, Luiten P, Eisel U, Van der Zee E. Both exposure to a novel context and associative learning induce an upregulation of AKAP150 protein in mouse hippocampus. Neurobiol Learn Mem. 2007;87:693-6 pubmed
    A-kinase anchoring protein 150 (AKAP150) is a multi-enzyme signaling complex that coordinates the action of PKA, PKC, and PP2B at neuronal membranes and synapses...
  11. Zhang J, Bal M, Bierbower S, Zaika O, Shapiro M. AKAP79/150 signal complexes in G-protein modulation of neuronal ion channels. J Neurosci. 2011;31:7199-211 pubmed publisher
    ..into rat SCG neurons of ?A-AKAP79, which lacks the A domain necessary for PKC binding, or the absence of AKAP150 in AKAP150(-/-) mice, did not affect I(M) suppression by purinergic agonist or by bradykinin, but reduced I(M) ..
  12. Marshall M, Clark J, Westenbroek R, Yu F, Scheuer T, Catterall W. Functional roles of a C-terminal signaling complex of CaV1 channels and A-kinase anchoring protein 15 in brain neurons. J Biol Chem. 2011;286:12627-39 pubmed publisher
    ..2 channels in vivo: increased functional expression, anchoring of AKAP15 and PKA, and initiation of excitation-transcription coupling. ..
  13. Lu Y, Zha X, Kim E, Schachtele S, Dailey M, Hall D, et al. A kinase anchor protein 150 (AKAP150)-associated protein kinase A limits dendritic spine density. J Biol Chem. 2011;286:26496-506 pubmed publisher
    The A kinase anchor protein AKAP150 recruits the cAMP-dependent protein kinase (PKA) to dendritic spines...
  14. Chen Q, Weiner R, Blackman B. Decreased expression of A-kinase anchoring protein 150 in GT1 neurons decreases neuron excitability and frequency of intrinsic gonadotropin-releasing hormone pulses. Endocrinology. 2010;151:281-90 pubmed publisher
    ..In neurons AKAP150 organizes many of the signaling molecules known to regulate the excitability and intrinsic pulsatile activity of ..
  15. Schnizler K, Shutov L, Van Kanegan M, Merrill M, Nichols B, McKnight G, et al. Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons. J Neurosci. 2008;28:4904-17 pubmed publisher
    ..Here, we demonstrate the critical role of the A-kinase anchoring protein AKAP150 in PKA-dependent modulation of TRPV1 function in adult mouse dorsal root ganglion (DRG) neurons...
  16. Efendiev R, Bavencoffe A, Hu H, Zhu M, Dessauer C. Scaffolding by A-kinase anchoring protein enhances functional coupling between adenylyl cyclase and TRPV1 channel. J Biol Chem. 2013;288:3929-37 pubmed publisher
    ..Significant AC activity is found associated with TRPV1 in dorsal root ganglia. The dissociation of AC from an AKAP150-TRPV1 complex in dorsal root ganglia neurons abolishes sensitization of TRPV1 induced by forskolin and ..
  17. Nijholt I, Ostroveanu A, Scheper W, Penke B, Luiten P, Van der Zee E, et al. Inhibition of PKA anchoring to A-kinase anchoring proteins impairs consolidation and facilitates extinction of contextual fear memories. Neurobiol Learn Mem. 2008;90:223-9 pubmed publisher
  18. Murphy J, Sanderson J, Gorski J, Scott J, Catterall W, Sather W, et al. AKAP-anchored PKA maintains neuronal L-type calcium channel activity and NFAT transcriptional signaling. Cell Rep. 2014;7:1577-1588 pubmed publisher
    ..Thus, LTCC-NFAT transcriptional signaling in neurons requires precise organization and balancing of PKA and CaN activities in the channel nanoenvironment, which is only made possible by AKAP79/150 scaffolding. ..
  19. Nichols C, Rossow C, Navedo M, Westenbroek R, Catterall W, Santana L, et al. Sympathetic stimulation of adult cardiomyocytes requires association of AKAP5 with a subpopulation of L-type calcium channels. Circ Res. 2010;107:747-56 pubmed publisher
    ..We hypothesized that scaffolding of cAMP signaling complexes by AKAP5 is required for efficient sympathetic stimulation of calcium transients.
  20. Nikandrova Y, Jiao Y, Baucum A, Tavalin S, Colbran R. Ca2+/calmodulin-dependent protein kinase II binds to and phosphorylates a specific SAP97 splice variant to disrupt association with AKAP79/150 and modulate alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor (AMPAR) activ. J Biol Chem. 2010;285:923-34 pubmed publisher
  21. Li L, Li J, Drum B, Chen Y, Yin H, Guo X, et al. Loss of AKAP150 promotes pathological remodelling and heart failure propensity by disrupting calcium cycling and contractile reserve. Cardiovasc Res. 2017;113:147-159 pubmed publisher
    ..The A-Kinase anchoring protein AKAP150 has been shown to coordinate key aspects of adrenergic regulation of Ca2+?cycling and excitation-..
  22. Han C, Tomita H, Ohba T, Nishizaki K, Ogata Y, Matsuzaki Y, et al. Modified sympathetic nerve regulation in AKAP5-null mice. Biochem Biophys Res Commun. 2016;469:897-902 pubmed publisher
    ..b>AKAP5, also known as AKAP79/150, is an anchoring protein between PKA and voltage-dependent calcium channels, ryanodine ..
  23. Jain A, Liu R, Ramani B, Arauz E, Ishitsuka Y, Ragunathan K, et al. Probing cellular protein complexes using single-molecule pull-down. Nature. 2011;473:484-8 pubmed publisher
    ..SiMPull should provide a rapid, sensitive and robust platform for analysing protein assemblies in biological pathways. ..
  24. Li X, Matta S, Sullivan R, Bahouth S. Carvedilol reverses cardiac insufficiency in AKAP5 knockout mice by normalizing the activities of calcineurin and CaMKII. Cardiovasc Res. 2014;104:270-9 pubmed publisher
    ..The scaffolding protein A-kinase anchoring protein 79/150 (AKAP5) is a key regulator of myocardial signalling by β-ARs...
  25. Nystoriak M, Nieves Cintrón M, Nygren P, Hinke S, Nichols C, Chen C, et al. AKAP150 contributes to enhanced vascular tone by facilitating large-conductance Ca2+-activated K+ channel remodeling in hyperglycemia and diabetes mellitus. Circ Res. 2014;114:607-15 pubmed publisher
    ..The scaffolding protein A-kinase anchoring protein 150 (AKAP150) is a key regulator of calcineurin (CaN), a phosphatase known to modulate the expression of the regulatory BKCa ?..
  26. Sonkusare S, Dalsgaard T, Bonev A, Hill Eubanks D, Kotlikoff M, Scott J, et al. AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is disrupted in hypertension. Sci Signal. 2014;7:ra66 pubmed publisher
    ..Muscarinic receptor-mediated activation of TRPV4 depended on protein kinase C (PKC) and the PKC-anchoring protein AKAP150, which was concentrated at MEPs...
  27. Jones B, Brunet S, Gilbert M, Nichols C, Su T, Westenbroek R, et al. Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation. Proc Natl Acad Sci U S A. 2012;109:17099-104 pubmed publisher
    ..Immunoblot and RT-PCR revealed that only the long isoforms of AKAP7 were detectable in ventricular cardiomyocytes. The results indicate that AKAP7 is not required for regulation of Ca(2+) handling in mouse cardiomyocytes. ..
  28. Nieves Cintrón M, Hirenallur Shanthappa D, Nygren P, Hinke S, Dell Acqua M, Langeberg L, et al. AKAP150 participates in calcineurin/NFAT activation during the down-regulation of voltage-gated K(+) currents in ventricular myocytes following myocardial infarction. Cell Signal. 2016;28:733-40 pubmed publisher
    ..hypothesis that sequestering of calcineurin to the sarcolemma of ventricular myocytes by the anchoring protein AKAP150 is required for acute activation of NFATc3 and the concomitant down-regulation of Kv channels following MI...
  29. Hinke S, Navedo M, Ulman A, Whiting J, Nygren P, Tian G, et al. Anchored phosphatases modulate glucose homeostasis. EMBO J. 2012;31:3991-4004 pubmed publisher
    ..islet studies and live-?-cell imaging approaches reveal that ablation of the kinase/phosphatase anchoring protein AKAP150 impairs insulin secretion in mice...
  30. Lin L, Sun W, Wikenheiser A, Kung F, Hoffman D. KChIP4a regulates Kv4.2 channel trafficking through PKA phosphorylation. Mol Cell Neurosci. 2010;43:315-25 pubmed publisher
    ..2 binding partners, allowing for discrete local PKA signaling. These data demonstrate that PKA phosphorylation of Kv4.2 plays an important role in the trafficking of Kv4.2 through its specific interaction with KChIP4a. ..
  31. Nystoriak M, Nieves Cintrón M, Patriarchi T, Buonarati O, Prada M, Morotti S, et al. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci Signal. 2017;10: pubmed publisher
    ..This arrangement depended upon scaffolding of PKA by an A-kinase anchoring protein 150 (AKAP150) in mice. Glucose-mediated increases in CaV1...
  32. Sanderson J, Scott J, Dell Acqua M. Control of Homeostatic Synaptic Plasticity by AKAP-Anchored Kinase and Phosphatase Regulation of Ca2+-Permeable AMPA Receptors. J Neurosci. 2018;38:2863-2876 pubmed publisher
    ..Here, using both male and female knock-in mice that are deficient in PKA or CaN anchoring, we show that AKAP150-anchored PKA and CaN also play novel roles in controlling CP-AMPAR synaptic incorporation during homeostatic ..
  33. Szteyn K, Rowan M, Gomez R, Du J, Carlton S, Jeske N. A-kinase anchoring protein 79/150 coordinates metabotropic glutamate receptor sensitization of peripheral sensory neurons. Pain. 2015;156:2364-72 pubmed publisher
    ..Herein, we identify that scaffolding protein A-kinase anchoring protein 79/150 (AKAP150) coordinates increased peripheral thermal sensitivity after group I metabotropic receptor (mGluR5) activation...