Gene Symbol: Actn1
Description: actinin, alpha 1
Alias: 3110023F10Rik, Actn1a, alpha-actinin-1, F-actin cross-linking protein, alpha actinin 1a, alpha-actinin cytoskeletal isoform, non-muscle alpha-actinin-1
Species: mouse
Products:     Actn1

Top Publications

  1. Bois P, O Hara B, Nietlispach D, Kirkpatrick J, Izard T. The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin. J Biol Chem. 2006;281:7228-36 pubmed
  2. Edlund M, Lotano M, Otey C. Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein. Cell Motil Cytoskeleton. 2001;48:190-200 pubmed
    ..Additionally, the exchange rates revealed by video-microscopy and FRAP analysis of alpha AGFP are more rapid than those reported previously, which were obtained using microinjection of large excesses of fluorescently-tagged protein. ..
  3. Keicher C, Gambaryan S, Schulze E, Marcus K, Meyer H, Butt E. Phosphorylation of mouse LASP-1 on threonine 156 by cAMP- and cGMP-dependent protein kinase. Biochem Biophys Res Commun. 2004;324:308-16 pubmed
    ..Immunoblotting of LASP-1 in various mouse and human tissues detected a similar prominent expression in non-muscle tissue. Altogether, our data suggest so far no functional differences between human and mouse LASP-1. ..
  4. Salucci S, Baldassarri V, Falcieri E, Burattini S. α-Actinin involvement in Z-disk assembly during skeletal muscle C2C12 cells in vitro differentiation. Micron. 2015;68:47-53 pubmed publisher
    ..These findings evidence α-actinin involvement in sarcomeric development, suggesting for this protein an important role in stabilizing the muscle contractile apparatus. ..
  5. Kos C, Le T, Sinha S, Henderson J, Kim S, Sugimoto H, et al. Mice deficient in alpha-actinin-4 have severe glomerular disease. J Clin Invest. 2003;111:1683-90 pubmed
    ..In addition, these genetic studies demonstrate that the nonsarcomeric alpha-actinin-4 is involved in the regulation of cell movement. ..
  6. Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, et al. MURC, a muscle-restricted coiled-coil protein that modulates the Rho/ROCK pathway, induces cardiac dysfunction and conduction disturbance. Mol Cell Biol. 2008;28:3424-36 pubmed publisher
    ..These findings indicate that MURC modulates RhoA signaling and that MURC plays an important role in the development of cardiac dysfunction and conduction disturbance with increased vulnerability to atrial arrhythmias. ..
  7. Zhang B, Xiao W, Qiu H, Zhang F, Moniz H, Jaworski A, et al. Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital diaphragmatic hernia. J Clin Invest. 2014;124:209-21 pubmed publisher
    ..Thus, our results demonstrate that loss of NDST1 causes defective diaphragm vascular development and CDH and that heparan sulfate facilitates angiogenic SLIT3-ROBO4 signaling during vascular development. ..
  8. Chen J, Huang Z, Seok H, Ding J, Kataoka M, Zhang Z, et al. mir-17-92 cluster is required for and sufficient to induce cardiomyocyte proliferation in postnatal and adult hearts. Circ Res. 2013;112:1557-66 pubmed publisher
    ..Our studies therefore identify miR-17-92 as a critical regulator of cardiomyocyte proliferation, and suggest this cluster of microRNAs could become therapeutic targets for cardiac repair and heart regeneration. ..
  9. Sakai Y, Shaw C, Dawson B, Dugas D, Al Mohtaseb Z, Hill D, et al. Protein interactome reveals converging molecular pathways among autism disorders. Sci Transl Med. 2011;3:86ra49 pubmed publisher
    ..The protein interaction network thus provides a framework for identifying causes of idiopathic autism and for understanding molecular pathways that underpin both syndromic and idiopathic ASDs. ..

More Information


  1. Fan Q, Xing Y, Ding J, Guan N, Zhang J. The relationship among nephrin, podocin, CD2AP, and alpha-actinin might not be a true 'interaction' in podocyte. Kidney Int. 2006;69:1207-15 pubmed
    ..There is not always an interaction between podocyte molecules. The normal localization of podocyte molecules would depend on their normal expression quantity and the molecular reactions between them. ..
  2. Harper B, Beckerle M, Pomies P. Fine mapping of the alpha-actinin binding site within cysteine-rich protein. Biochem J. 2000;350 Pt 1:269-74 pubmed
    ..The critical lysine residue localizes within a short alpha-helix, raising the possibility that mutagenesis-induced alterations in alpha-actinin-binding capacity might be attributed to the disruption of a key structural element. ..
  3. Lu Y, Zhang Y, Pan M, Kim N, Sun S, Cui X. Daam1 regulates fascin for actin assembly in mouse oocyte meiosis. Cell Cycle. 2017;16:1350-1356 pubmed publisher
    ..Thus, our data suggested that Daam1 affected actin assembly during oocyte meiotic division via the regulation of fascin expression. ..
  4. Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
    ..Our data demonstrate that the membrane protein Tmem65 is an intercalated disc protein that interacts with and functionally regulates ventricular Cx43. ..
  5. Rudolf R, Bogomolovas J, Strack S, Choi K, Khan M, Wagner A, et al. Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways. Age (Dordr). 2013;35:1663-74 pubmed publisher
    ..Our data demonstrate an involvement of MuRF1 in membrane protein-turnover, including the degradation of AChRs at the NMJ under atrophying conditions where MuRF1 also interacts and associates with Bif-1. ..
  6. Nakagawa N, Hoshijima M, Oyasu M, Saito N, Tanizawa K, Kuroda S. ENH, containing PDZ and LIM domains, heart/skeletal muscle-specific protein, associates with cytoskeletal proteins through the PDZ domain. Biochem Biophys Res Commun. 2000;272:505-12 pubmed
  7. Baldini G, Martelli A, Tabellini G, Horn C, Machaca K, Narducci P, et al. Rabphilin localizes with the cell actin cytoskeleton and stimulates association of granules with F-actin cross-linked by {alpha}-actinin. J Biol Chem. 2005;280:34974-84 pubmed
    ..These results suggest that rabphilin, by interacting with alpha-actinin, organizes the cell cytoskeleton to facilitate granule localization within F-actin-rich regions. ..
  8. Pashmforoush M, Pomies P, Peterson K, Kubalak S, Ross J, Hefti A, et al. Adult mice deficient in actinin-associated LIM-domain protein reveal a developmental pathway for right ventricular cardiomyopathy. Nat Med. 2001;7:591-7 pubmed
    ..Taken together, these studies point to a novel developmental pathway for RV dilated cardiomyopathy via instability of alpha-actinin complexes. ..
  9. Burgueno J, Blake D, Benson M, Tinsley C, Esapa C, Canela E, et al. The adenosine A2A receptor interacts with the actin-binding protein alpha-actinin. J Biol Chem. 2003;278:37545-52 pubmed
    ..These findings suggest an alpha-actinin-dependent association between the actin cytoskeleton and A2AR trafficking. ..
  10. Gokhin D, Lewis R, McKeown C, Nowak R, Kim N, Littlefield R, et al. Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology. J Cell Biol. 2010;189:95-109 pubmed publisher
    ..Thus, Tmod3 and -4 compensate for the absence of Tmod1 structurally but not functionally. We conclude that Tmod1 is a novel regulator of skeletal muscle physiology. ..
  11. Conti F, Monkley S, Wood M, Critchley D, Muller U. Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions. Development. 2009;136:3597-606 pubmed publisher
    ..We conclude that talin 1 and 2 are crucial for skeletal muscle development, where they regulate myoblast fusion, sarcomere assembly and the maintenance of MTJs. ..
  12. Calaway J, Domínguez J, Hanson M, Cambranis E, Pardo Manuel de Villena F, de la Casa Esperón E. Intronic parent-of-origin dependent differential methylation at the Actn1 gene is conserved in rodents but is not associated with imprinted expression. PLoS ONE. 2012;7:e48936 pubmed publisher
    ..found a novel differentially methylated region in a CpG island located in the last intron of the alpha 1 Actinin (Actn1) gene...
  13. Yadav R, Vattepu R, Beck M. Phosphoinositide Binding Inhibits Actin Crosslinking and Polymerization by Palladin. J Mol Biol. 2016;428:4031-4047 pubmed publisher
    ..Our results provide biochemical proof that PI(4,5)P2 functions as a moderator of palladin activity and have also identified residues directly involved in the crosslinking activity of palladin. ..
  14. Cabello N, Remelli R, Canela L, Soriguera A, Mallol J, Canela E, et al. Actin-binding protein alpha-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor. J Biol Chem. 2007;282:12143-53 pubmed
    ..Together, these findings indicate that there is an alpha-actinin-1-dependent mGlu(5b) receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning. ..
  15. Tessari A, Pietrobon M, Notte A, Cifelli G, Gage P, Schneider M, et al. Myocardial Pitx2 differentially regulates the left atrial identity and ventricular asymmetric remodeling programs. Circ Res. 2008;102:813-22 pubmed publisher
    ..This is the first evidence for distinct Pitx2 action in mediating L/R atrial identity and asymmetrical ventricular remodeling. ..
  16. Roca Cusachs P, Del Rio A, Puklin Faucher E, Gauthier N, Biais N, Sheetz M. Integrin-dependent force transmission to the extracellular matrix by ?-actinin triggers adhesion maturation. Proc Natl Acad Sci U S A. 2013;110:E1361-70 pubmed publisher
    ..Such a multistep process enables cells to adjust forces on matrices, unveiling a role of ?-actinin that is different from its well-studied function as an actin cross-linker. ..
  17. Beck H, Flynn K, Lindenberg K, Schwarz H, Bradke F, Di Giovanni S, et al. Serum Response Factor (SRF)-cofilin-actin signaling axis modulates mitochondrial dynamics. Proc Natl Acad Sci U S A. 2012;109:E2523-32 pubmed
    ..In summary, our data suggest that the SRF-cofilin-actin signaling axis modulates neuronal mitochondrial function. ..
  18. Koh T, Escobedo J. Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions. Am J Physiol Cell Physiol. 2004;286:C713-22 pubmed
  19. Okina E, Grossi A, Gopal S, Multhaupt H, Couchman J. Alpha-actinin interactions with syndecan-4 are integral to fibroblast-matrix adhesion and regulate cytoskeletal architecture. Int J Biochem Cell Biol. 2012;44:2161-74 pubmed publisher
    ..These data provide an additional dimension to syndecan function related to tension at the cell-matrix interface, wound healing and potentially fibrosis...
  20. Beqqali A, Monshouwer Kloots J, Monteiro R, Welling M, Bakkers J, Ehler E, et al. CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. J Cell Sci. 2010;123:1141-50 pubmed publisher
    ..These findings suggest that CHAP is a critical component of the sarcomere with an important role in muscle development. ..
  21. Di Biase V, Obermair G, Szabo Z, Altier C, Sanguesa J, Bourinet E, et al. Stable membrane expression of postsynaptic CaV1.2 calcium channel clusters is independent of interactions with AKAP79/150 and PDZ proteins. J Neurosci. 2008;28:13845-55 pubmed publisher
    ..2 is independent of its interactions with AKAP79/150 and PDZ proteins. ..
  22. Domínguez J, Navarro F, Franco D, Thompson R, Aranega A. Temporal and spatial expression pattern of beta1 sodium channel subunit during heart development. Cardiovasc Res. 2005;65:842-50 pubmed
    ..These results demonstrate that Scn1b is expressed during mouse heart development, suggesting it can play an important role in the action potential configuration of the cardiomyocytes during heart morphogenesis. ..
  23. Zhao Z, Deocharan B, Scherer P, Ozelius L, Putterman C. Differential binding of cross-reactive anti-DNA antibodies to mesangial cells: the role of alpha-actinin. J Immunol. 2006;176:7704-14 pubmed
    ..Modulation of Ag expression may be a promising approach to down-regulate immune complex formation in the target organ in individuals with circulating pathogenic Abs. ..
  24. Sanger J, Wang J, Gleason L, Chowrashi P, Dube D, Mittal B, et al. Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules. Cytoskeleton (Hoboken). 2010;67:808-23 pubmed publisher
    ..Our results indicate that ArgBP2 may play an important role in the assembly and maintenance of myofibrils in cardiomyocytes. ..
  25. Nygren J, Liuba K, Breitbach M, Stott S, Thorén L, Roell W, et al. Myeloid and lymphoid contribution to non-haematopoietic lineages through irradiation-induced heterotypic cell fusion. Nat Cell Biol. 2008;10:584-92 pubmed publisher
    ..Our findings demonstrate that blood cells of the lymphoid and myeloid lineages contribute to various non-haematopoietic tissues by forming rare fusion hybrids, but almost exclusively in response to injuries or inflammation. ..
  26. Guo W, Wang Y. A three-component mechanism for fibroblast migration with a contractile cell body that couples a myosin II-independent propulsive anterior to a myosin II-dependent resistive tail. Mol Biol Cell. 2012;23:1657-63 pubmed publisher
  27. Torii T, Miyamoto Y, Nakamura K, Maeda M, Yamauchi J, Tanoue A. Arf6 guanine-nucleotide exchange factor, cytohesin-2, interacts with actinin-1 to regulate neurite extension. Cell Signal. 2012;24:1872-82 pubmed publisher
    ..guanine-nucleotide exchange factor (GEF), cytohesin-2 (CYTH2), interacts with the cytoskeletal protein actinin-1 (ACTN1) and regulates neurite extension in N1E-115 cells used as the model...
  28. Hall D, Dai S, Tseng P, Malik Z, Nguyen M, Matt L, et al. Competition between ?-actinin and Ca²?-calmodulin controls surface retention of the L-type Ca²? channel Ca(V)1.2. Neuron. 2013;78:483-97 pubmed publisher
    ..2 at the plasma membrane and that its displacement by Ca²?-calmodulin triggers Ca²?-induced endocytosis of Ca(V)1.2, thus providing an important negative feedback mechanism for Ca²? influx. ..
  29. Ryu J, Kim H, Lee S, Chang E, Kim H, Kim H. Proteomic identification of the TRAF6 regulation of vacuolar ATPase for osteoclast function. Proteomics. 2005;5:4152-60 pubmed
    ..Our study identified V-ATPase as a TRAF6-binding protein using a proteomics strategy and proved a direct link between these two important molecules for osteoclast function. ..
  30. Sun Y, Liang X, Najafi N, Cass M, Lin L, Cai C, et al. Islet 1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells. Dev Biol. 2007;304:286-96 pubmed
    ..Our studies suggest a role for Isl1 in these distinct domains of expression within the heart. ..
  31. Zhang J, Elzey B, Williams G, Lu S, Law D, Horowits R. Ultrastructural and biochemical localization of N-RAP at the interface between myofibrils and intercalated disks in the mouse heart. Biochemistry. 2001;40:14898-906 pubmed
    ..These results demonstrate that N-RAP remains tightly bound to myofibrils and fasciae adherentes during biochemical purification and may be a key constituent in the mechanical link between these two structures. ..
  32. Dhavan R, Greer P, Morabito M, Orlando L, Tsai L. The cyclin-dependent kinase 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent manner. J Neurosci. 2002;22:7879-91 pubmed
  33. Zagore L, Grabinski S, Sweet T, Hannigan M, Sramkoski R, Li Q, et al. RNA Binding Protein Ptbp2 Is Essential for Male Germ Cell Development. Mol Cell Biol. 2015;35:4030-42 pubmed publisher
    ..Collectively, the data provide evidence for an important role for alternative splicing regulation in germ cell development and a central role for Ptbp2 in this process. ..
  34. Foley K, Young P. An analysis of splicing, actin-binding properties, heterodimerization and molecular interactions of the non-muscle ?-actinins. Biochem J. 2013;452:477-88 pubmed publisher
    ..This significantly alters our view of non-muscle actinin function. ..
  35. Vincent P, Bouleau Y, Petit C, Dulon D. A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells. elife. 2015;4: pubmed publisher
  36. Sakane A, Alamir Mahmoud Abdallah A, Nakano K, Honda K, Kitamura T, Imoto I, et al. Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins. Genes Cells. 2013;18:810-22 pubmed publisher
    ..However, actinin-1/-4 have no effect on the cell spreading regulated by JRAB?CC. These data suggest that JRAB contributes to the rearrangement of the actin cytoskeleton during cell spreading via filamins. ..
  37. Besco J, Hooft van Huijsduijnen R, Frostholm A, Rotter A. Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT). Brain Res. 2006;1116:50-7 pubmed
  38. Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, et al. Synaptopodin regulates the actin-bundling activity of alpha-actinin in an isoform-specific manner. J Clin Invest. 2005;115:1188-98 pubmed
    ..In concert, synaptopodin regulates the actin-bundling activity of alpha-actinin in highly dynamic cell compartments, such as podocyte FPs and the dendritic spine apparatus. ..
  39. Voelkel T, Andresen C, Unger A, Just S, Rottbauer W, Linke W. Lysine methyltransferase Smyd2 regulates Hsp90-mediated protection of the sarcomeric titin springs and cardiac function. Biochim Biophys Acta. 2013;1833:812-22 pubmed publisher
    ..This article is part of a Special Issue entitled: Cardiomyocyte Biology: Cardiac Pathways of Differentiation, Metabolism and Contraction. ..
  40. Collin G, Marshall J, King B, Milan G, Maffei P, Jagger D, et al. The Alström syndrome protein, ALMS1, interacts with ?-actinin and components of the endosome recycling pathway. PLoS ONE. 2012;7:e37925 pubmed publisher
    ..Our results suggest a role for ALMS1 variants in the recycling endosome pathway and give us new insights into the pathogenesis of a subset of clinical phenotypes associated with ALMS. ..
  41. Heineke J, Ruetten H, Willenbockel C, Gross S, Naguib M, Schaefer A, et al. Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc. Proc Natl Acad Sci U S A. 2005;102:1655-60 pubmed
    ..Our study reveals a link between the stress sensor MLP and the calcineurin-NFAT pathway at the sarcomeric Z-disk in cardiomyocytes and indicates that reduced MLP-calcineurin signaling predisposes to adverse remodeling after MI. ..
  42. Sakagami H, Honma T, Sukegawa J, Owada Y, Yanagisawa T, Kondo H. Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines. Eur J Neurosci. 2007;25:618-28 pubmed
    ..Our present findings suggest that EFA6A may form a protein complex with alpha-actinin and activate ARF6 in close proximity of the actin cytoskeleton and membrane proteins in the dendritic spines. ..
  43. Yuan B, Wan P, Chu D, Nie J, Cao Y, Luo W, et al. A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice. Am J Pathol. 2014;184:1967-80 pubmed publisher
    ..Taken together, these results demonstrate that AIP1-regulated actin dynamics play essential roles in heart function in mice. ..
  44. Lang G, White J, Argent Katwala M, Allinson C, Weston K. Myb proteins regulate the expression of diverse target genes. Oncogene. 2005;24:1375-84 pubmed
    ..We present here a novel group of 29 validated Myb family target genes of diverse functions. ..
  45. Hao J, Galindo C, Tran T, Sawyer D. Neuregulin-1? induces embryonic stem cell cardiomyogenesis via ErbB3/ErbB2 receptors. Biochem J. 2014;458:335-41 pubmed publisher
    ..In summary, we report a previously unrecognized role for NRG-1?/ErbB3/CREB signalling at the pre-mesoderm stage for stem cell cardiac differentiation. ..
  46. Petchey L, Risebro C, Vieira J, Roberts T, Bryson J, Greensmith L, et al. Loss of Prox1 in striated muscle causes slow to fast skeletal muscle fiber conversion and dilated cardiomyopathy. Proc Natl Acad Sci U S A. 2014;111:9515-20 pubmed publisher
    ..Our study identifies conserved roles for Prox1 between cardiac and skeletal muscle, specifically implicated in slow-twitch fiber-type specification, function, and cardiomyopathic disease. ..
  47. Yang N, Schindeler A, McDonald M, Seto J, Houweling P, Lek M, et al. ?-Actinin-3 deficiency is associated with reduced bone mass in human and mouse. Bone. 2011;49:790-8 pubmed publisher
    ..Given the high frequency of R577X in the general population, the potential role of ACTN3 R577X as a factor influencing variations in BMD in elderly humans warrants further study. ..
  48. Frey N, Richardson J, Olson E. Calsarcins, a novel family of sarcomeric calcineurin-binding proteins. Proc Natl Acad Sci U S A. 2000;97:14632-7 pubmed
    ..Calsarcins represent a novel family of sarcomeric proteins that link calcineurin with the contractile apparatus, thereby potentially coupling muscle activity to calcineurin activation. ..
  49. Barrientos T, Frank D, Kuwahara K, Bezprozvannaya S, Pipes G, Bassel Duby R, et al. Two novel members of the ABLIM protein family, ABLIM-2 and -3, associate with STARS and directly bind F-actin. J Biol Chem. 2007;282:8393-403 pubmed
    ..These findings suggest that the members of the novel ABLIM protein family may serve as a scaffold for signaling modules of the actin cytoskeleton and thereby modulate transcription. ..