Acat2

Summary

Gene Symbol: Acat2
Description: acetyl-Coenzyme A acetyltransferase 2
Alias: AW742799, Tcp-1x, Tcp1-rs1, acetyl-CoA acetyltransferase, cytosolic, cytosolic acetoacetyl-CoA thiolase, t-complex protein 1, related sequence 1
Species: mouse
Products:     Acat2

Top Publications

  1. Buhman K, Accad M, Novak S, Choi R, Wong J, Hamilton R, et al. Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice. Nat Med. 2000;6:1341-7 pubmed
    ..We now demonstrate that ACAT2 is the major ACAT in mouse small intestine and liver, and suggest that ACAT2 deficiency has profound effects on ..
  2. Temel R, Lee R, Kelley K, Davis M, Shah R, Sawyer J, et al. Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2. J Lipid Res. 2005;46:2423-31 pubmed
    ..Because of its ability to esterify cholesterol for packaging into nascent chylomicrons, ACAT2 plays an important role in cholesterol absorption...
  3. Alger H, Brown J, Sawyer J, Kelley K, Shah R, Wilson M, et al. Inhibition of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) prevents dietary cholesterol-associated steatosis by enhancing hepatic triglyceride mobilization. J Biol Chem. 2010;285:14267-74 pubmed publisher
    Acyl-CoA:cholesterol O-acyl transferase 2 (ACAT2) promotes cholesterol absorption by the intestine and the secretion of cholesteryl ester-enriched very low density lipoproteins by the liver...
  4. Brown J, Bell T, Alger H, Sawyer J, Smith T, Kelley K, et al. Targeted depletion of hepatic ACAT2-driven cholesterol esterification reveals a non-biliary route for fecal neutral sterol loss. J Biol Chem. 2008;283:10522-34 pubmed publisher
    Deletion of acyl-CoA:cholesterol O-acyltransferase 2 (ACAT2) in mice results in resistance to diet-induced hypercholesterolemia and protection against atherosclerosis...
  5. Lee R, Shah R, Sawyer J, Hamilton R, Parks J, Rudel L. ACAT2 contributes cholesteryl esters to newly secreted VLDL, whereas LCAT adds cholesteryl ester to LDL in mice. J Lipid Res. 2005;46:1205-12 pubmed
    The relative contributions of ACAT2 and LCAT to the cholesteryl ester (CE) content of VLDL and LDL were measured. ACAT2 deficiency led to a significant decrease in the percentage of CE (37.2 +/- 2.1% vs. 3.9 +/- 0...
  6. Zhang J, Kelley K, Marshall S, Davis M, Wilson M, Sawyer J, et al. Tissue-specific knockouts of ACAT2 reveal that intestinal depletion is sufficient to prevent diet-induced cholesterol accumulation in the liver and blood. J Lipid Res. 2012;53:1144-52 pubmed publisher
    Acyl-CoA:cholesterol acyltransferase 2 (ACAT2) generates cholesterol esters (CE) for packaging into newly synthesized lipoproteins and thus is a major determinant of blood cholesterol levels...
  7. Dixon J, Kim Y, Brinker A, Quadro L. Loss of ?-carotene 15,15'-oxygenase in developing mouse tissues alters esterification of retinol, cholesterol and diacylglycerols. Biochim Biophys Acta. 2014;1841:34-43 pubmed publisher
  8. Schweifer N, Valk P, Delwel R, Cox R, Francis F, Meier Ewert S, et al. Characterization of the C3 YAC contig from proximal mouse chromosome 17 and analysis of allelic expression of genes flanking the imprinted Igf2r gene. Genomics. 1997;43:285-97 pubmed
    ..The remaining genes in this region have previously been shown to be biallelically expressed. ..
  9. Willison K, Dudley K, Potter J. Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell. 1986;44:727-38 pubmed
    ..DNA sequence comparison of the Tcp-1 genes suggests that the t haplotype chromosome arose within the genus Mus more than one million years ago. ..

More Information

Publications22

  1. Nerurkar P, Schut H, Anderson L, Riggs C, Snyderwine E, Thorgeirsson S, et al. DNA adducts of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in colon, bladder, and kidney of congenic mice differing in Ah responsiveness and N-acetyltransferase genotype. Cancer Res. 1995;55:3043-9 pubmed
    ..5-fold increase in adducts but only in the Ah-responsive, rapid acetylator mice. In sum, these polymorphisms influenced the level of IQ-DNA adducts in the kidney, urinary bladder, and colon in complex ways. ..
  2. Nguyen T, Sawyer J, Kelley K, Davis M, Rudel L. Cholesterol esterification by ACAT2 is essential for efficient intestinal cholesterol absorption: evidence from thoracic lymph duct cannulation. J Lipid Res. 2012;53:95-104 pubmed publisher
    The hypothesis tested in this study was that cholesterol esterification by ACAT2 would increase cholesterol absorption efficiency by providing cholesteryl ester (CE) for incorporation into chylomicrons...
  3. Zwart R, Verhaagh S, De Jong J, Lyon M, Barlow D. Genetic analysis of the organic cation transporter genes Orct2/Slc22a2 and Orct3/Slc22a3 reduces the critical region for the t haplotype mutant t(w73) to 200 kb. Mamm Genome. 2001;12:734-40 pubmed
    ..The results eliminate both Orct2 and Orct3 as candidates and further reduce the critical region containing the t(w73) mutant from 500 kb to 200 kb. ..
  4. Ashworth A. Two acetyl-CoA acetyltransferase genes located in the t-complex region of mouse chromosome 17 partially overlap the Tcp-1 and Tcp-1x genes. Genomics. 1993;18:195-8 pubmed
    ..Both Acat genes appear to be transcribed in several mouse tissues. An ACAT gene also overlaps the TCP1 gene in humans, suggesting that this close linkage may have some functional significance. ..
  5. Dudley K, Shanahan F, Burtenshaw M, Evans E, Ruddy S, Lyon M. Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x. Genet Res. 1991;57:147-52 pubmed
    ..A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1. ..
  6. Himmelbauer H, Artzt K, Barlow D, Fischer Lindahl K, Lyon M, Klein J, et al. Encyclopedia of the mouse genome III. October 1993. Mouse chromosome 17. Mamm Genome. 1993;4 Spec No:S230-52 pubmed
  7. Ohshiro T, Matsuda D, Sakai K, Degirolamo C, Yagyu H, Rudel L, et al. Pyripyropene A, an acyl-coenzyme A:cholesterol acyltransferase 2-selective inhibitor, attenuates hypercholesterolemia and atherosclerosis in murine models of hyperlipidemia. Arterioscler Thromb Vasc Biol. 2011;31:1108-15 pubmed publisher
    ..compound that has been found to strongly and selectively inhibit acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) isozyme activity in vitro...
  8. Rogers M, Liu J, Kushnir M, Bryleva E, Rockwood A, Meikle A, et al. Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase. J Biol Chem. 2012;287:17483-92 pubmed publisher
    ..Acyl-CoA:cholesterol acyltransferase 1 and 2 (ACAT1 and ACAT2) convert various sterols to steryl esters; their activities are activated by cholesterol...
  9. Nguyen T, Sawyer J, Kelley K, Davis M, Kent C, Rudel L. ACAT2 and ABCG5/G8 are both required for efficient cholesterol absorption in mice: evidence from thoracic lymph duct cannulation. J Lipid Res. 2012;53:1598-609 pubmed publisher
    ..cassette transporter G5 and G8 heterodimer (G5G8), and acyl CoA:cholesterol acyltransferase 2 (ACAT2)...
  10. Pedrelli M, Davoodpour P, Degirolamo C, Gomaraschi M, Graham M, Ossoli A, et al. Hepatic ACAT2 knock down increases ABCA1 and modifies HDL metabolism in mice. PLoS ONE. 2014;9:e93552 pubmed publisher
    b>ACAT2 is the exclusive cholesterol-esterifying enzyme in hepatocytes and enterocytes. Hepatic ABCA1 transfers unesterified cholesterol (UC) to apoAI, thus generating HDL...
  11. Wang Y, Bian Y, Luo J, Lu M, Xiong Y, Guo S, et al. Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation. Nat Cell Biol. 2017;19:808-819 pubmed publisher
    ..However, the physiological regulation of cysteine ubiquitylation is still mysterious. Here we found that ACAT2, a cellular enzyme converting cholesterol and fatty acid to cholesteryl esters, was ubiquitylated on Cys277 for ..
  12. Iqbal J, Boutjdir M, Rudel L, Hussain M. Intestine-specific MTP and global ACAT2 deficiency lowers acute cholesterol absorption with chylomicrons and HDLs. J Lipid Res. 2014;55:2261-75 pubmed publisher
    ..Chylomicrons transport free and esterified cholesterol, whereas HDLs transport free cholesterol. ACAT2 esterifies cholesterol for secretion with chylomicrons...
  13. Kim Y, Zuccaro M, Costabile B, Rodas R, Quadro L. Tissue- and sex-specific effects of β-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism in adult and developing mice. Arch Biochem Biophys. 2015;572:11-8 pubmed publisher