Acat1

Summary

Gene Symbol: Acat1
Description: acetyl-Coenzyme A acetyltransferase 1
Alias: 6330585C21Rik, Acat, acetyl-CoA acetyltransferase, mitochondrial, acetoacetyl-CoA thiolase
Species: mouse
Products:     Acat1

Top Publications

  1. Huang L, Melton E, Li H, Sohn P, Rogers M, Mulligan Kehoe M, et al. Myeloid Acyl-CoA:Cholesterol Acyltransferase 1 Deficiency Reduces Lesion Macrophage Content and Suppresses Atherosclerosis Progression. J Biol Chem. 2016;291:6232-44 pubmed publisher
    Acyl-CoA:cholesterol acyltransferase 1 (Acat1) converts cellular cholesterol to cholesteryl esters and is considered a drug target for treating atherosclerosis...
  2. Fazio S, Major A, Swift L, Gleaves L, Accad M, Linton M, et al. Increased atherosclerosis in LDL receptor-null mice lacking ACAT1 in macrophages. J Clin Invest. 2001;107:163-71 pubmed
    ..We show here, however, that hypercholesterolemic LDL receptor-deficient (LDLR(-/-)) mice reconstituted with ACAT1-deficient macrophages unexpectedly develop larger atherosclerotic lesions than control LDLR(-/-) mice...
  3. Fan J, Shan C, Kang H, Elf S, Xie J, Tucker M, et al. Tyr phosphorylation of PDP1 toggles recruitment between ACAT1 and SIRT3 to regulate the pyruvate dehydrogenase complex. Mol Cell. 2014;53:534-48 pubmed publisher
    ..Moreover, we identified mitochondrial ACAT1 and SIRT3 as the upstream acetyltransferase and deacetylase, respectively, of PDHA1 and PDP1, while knockdown of ..
  4. Masuno M, Kano M, Fukao T, Yamaguchi S, Osumi T, Hashimoto T, et al. Chromosome mapping of the human mitochondrial acetoacetyl-coenzyme A thiolase gene to 11q22.3----q23.1 by fluorescence in situ hybridization. Cytogenet Cell Genet. 1992;60:121-2 pubmed
  5. Leader D, Gall I, Campbell P, Frischauf A. Isolation and characterization of cDNA clones from mouse skeletal muscle actin mRNA. DNA. 1986;5:235-8 pubmed
  6. Pecker I, Avraham K, Gilbert D, Savitsky K, Rotman G, Harnik R, et al. Identification and chromosomal localization of Atm, the mouse homolog of the ataxia-telangiectasia gene. Genomics. 1996;35:39-45 pubmed
    ..Fluorescence in situ hybridization and linkage analysis located the Atm gene on mouse chromosome 9, band 9C, in a region homologous to the ATM region on human chromosome 11q22-q23. ..
  7. Dixon J, Kim Y, Brinker A, Quadro L. Loss of ?-carotene 15,15'-oxygenase in developing mouse tissues alters esterification of retinol, cholesterol and diacylglycerols. Biochim Biophys Acta. 2014;1841:34-43 pubmed publisher
    ..Furthermore, LCAT, ACAT1 and DGAT2 mRNA levels were also downregulated in CMOI-/- embryos...
  8. Huttunen H, Peach C, Bhattacharyya R, Barren C, Pettingell W, Hutter Paier B, et al. Inhibition of acyl-coenzyme A: cholesterol acyl transferase modulates amyloid precursor protein trafficking in the early secretory pathway. FASEB J. 2009;23:3819-28 pubmed publisher
    ..Previous studies have identified acyl-coenzyme A: cholesterol acyltransferase (ACAT), an enzyme that regulates subcellular cholesterol distribution, as a potential therapeutic target for AD...
  9. Rogers M, Liu J, Kushnir M, Bryleva E, Rockwood A, Meikle A, et al. Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase. J Biol Chem. 2012;287:17483-92 pubmed publisher
    ..Acyl-CoA:cholesterol acyltransferase 1 and 2 (ACAT1 and ACAT2) convert various sterols to steryl esters; their activities are activated by cholesterol...

More Information

Publications35

  1. Uelmen P, Oka K, Sullivan M, Chang C, Chang T, Chan L. Tissue-specific expression and cholesterol regulation of acylcoenzyme A:cholesterol acyltransferase (ACAT) in mice. Molecular cloning of mouse ACAT cDNA, chromosomal localization, and regulation of ACAT in vivo and in vitro. J Biol Chem. 1995;270:26192-201 pubmed
    Acyl-coenzyme A:cholesterol acyltransferase (ACAT) catalyzes the esterification of cholesterol with long chain fatty acids and is believed to play an important part in the development of atherosclerotic lesions...
  2. Kim Y, Zuccaro M, Costabile B, Rodas R, Quadro L. Tissue- and sex-specific effects of β-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism in adult and developing mice. Arch Biochem Biophys. 2015;572:11-8 pubmed publisher
  3. Xia Y, Welch C, Warden C, Lange E, Fukao T, Lusis A, et al. Assignment of the mouse ataxia-telangiectasia gene (Atm) to mouse chromosome 9. Mamm Genome. 1996;7:554-5 pubmed
  4. Himmelbauer H, Artzt K, Barlow D, Fischer Lindahl K, Lyon M, Klein J, et al. Encyclopedia of the mouse genome III. October 1993. Mouse chromosome 17. Mamm Genome. 1993;4 Spec No:S230-52 pubmed
  5. Huang L, Gui J, Artinger E, Craig R, Berwin B, ERNST P, et al. Acat1 gene ablation in mice increases hematopoietic progenitor cell proliferation in bone marrow and causes leukocytosis. Arterioscler Thromb Vasc Biol. 2013;33:2081-7 pubmed publisher
    To investigate the role of acyl-CoA:cholesterol acyltransferase 1 (ACAT1) in hematopoiesis. ACAT1 converts cellular cholesterol to cholesteryl esters for storage in multiple cell types and is a potential drug target for human diseases...
  6. Temel R, Gebre A, Parks J, Rudel L. Compared with Acyl-CoA:cholesterol O-acyltransferase (ACAT) 1 and lecithin:cholesterol acyltransferase, ACAT2 displays the greatest capacity to differentiate cholesterol from sitosterol. J Biol Chem. 2003;278:47594-601 pubmed
    ..cholesterol from the plant sterol, sitosterol, was compared with that of the sterol esterifying enzymes, ACAT1 and lecithin:cholesterol acyltransferase (LCAT)...
  7. Yoshinaka Y, Shibata H, Kobayashi H, Kuriyama H, Shibuya K, Tanabe S, et al. A selective ACAT-1 inhibitor, K-604, stimulates collagen production in cultured smooth muscle cells and alters plaque phenotype in apolipoprotein E-knockout mice. Atherosclerosis. 2010;213:85-91 pubmed publisher
    Acyl-coenzyme A:cholesterol O-acyltransferase-1 (ACAT-1) plays an essential role in macrophage foam cell formation and progression of atherosclerosis...
  8. Wang B, He P, Zeng G, Zhang T, Ou Yang X. miR-467b regulates the cholesterol ester formation via targeting ACAT1 gene in RAW 264.7 macrophages. Biochimie. 2017;132:38-44 pubmed publisher
    ..Interestingly, computational analysis demonstrated that ACAT1 which converts intracellular FC into the storage form of CE, and ABCA1 which promotes cellular FC efflux may be ..
  9. Rodriguez A, Ashen M, Chen E. ACAT1 deletion in murine macrophages associated with cytotoxicity and decreased expression of collagen type 3A1. Biochem Biophys Res Commun. 2005;331:61-8 pubmed
    ..Herein, we examined foam cell formation and cytotoxicity in murine ACAT1 knockout (KO) macrophages in an attempt to resolve the discrepancies...
  10. McDaniel A, Li X, Tordoff M, Bachmanov A, Reed D. A locus on mouse Chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depot. Mamm Genome. 2006;17:1078-92 pubmed
    ..candidates within the confidence interval with functional significance to the body fatness phenotype (Il18, Acat1, Cyp19a1, Crabp1, Man2c1, Neil1, Mpi1, Csk, Lsm16, Adpgk, Bbs4, Hexa, Thsd4, Dpp8, Anxa2, and Lipc)...
  11. Matsuda Y, Imai T, Shiomi T, Saito T, Yamauchi M, Fukao T, et al. Comparative genome mapping of the ataxia-telangiectasia region in mouse, rat, and Syrian hamster. Genomics. 1996;34:347-52 pubmed
    Chromosomal locations of the Atm (ataxia-telangiectasia (AT)-mutated) and Acat1 (mitochondrial acetoacetyl-CoA thiolase) genes in mouse, rat, and Syrian hamster were determined by direct R-banding FISH...
  12. Yin Y, Liao S, Zhang M, Liu Y, Li B, Zhou Y, et al. TLR4-mediated inflammation promotes foam cell formation of vascular smooth muscle cell by upregulating ACAT1 expression. Cell Death Dis. 2014;5:e1574 pubmed publisher
    ..Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) promotes foam cell formation by promoting intracellular cholesteryl ester synthesis...
  13. Patel N, Hitzemann R. Detection and mapping of quantitative trait loci for haloperidol-induced catalepsy in a C57BL/6J x DBA/2J F2 intercross. Behav Genet. 1999;29:303-10 pubmed
    ..9 stretched from approximately 25 to 55 cM; this region contains numerous candidate genes, including Drd2, Ncam, Acat1, and Htr1b. The data also suggest the presence of a second QTL on chromosome 9 (LOD, > 3...
  14. Hyogo H, Roy S, Paigen B, Cohen D. Leptin promotes biliary cholesterol elimination during weight loss in ob/ob mice by regulating the enterohepatic circulation of bile salts. J Biol Chem. 2002;277:34117-24 pubmed
    ..Cholesterol gallstone formation during weight loss in ob/ob mice appears to represent a pathologic consequence of an adaptive response that prevents absorption of biliary and dietary cholesterol. ..
  15. Su Y, Dove D, Major A, Hasty A, Boone B, Linton M, et al. Reduced ABCA1-mediated cholesterol efflux and accelerated atherosclerosis in apolipoprotein E-deficient mice lacking macrophage-derived ACAT1. Circulation. 2005;111:2373-81 pubmed
    Macrophage acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) and apolipoprotein E (apoE) have been implicated in regulating cellular cholesterol homeostasis and therefore play critical roles in foam cell formation...
  16. Rozan L, El Deiry W. Identification and characterization of proteins interacting with Traf4, an enigmatic p53 target. Cancer Biol Ther. 2006;5:1228-35 pubmed
  17. Schweifer N, Valk P, Delwel R, Cox R, Francis F, Meier Ewert S, et al. Characterization of the C3 YAC contig from proximal mouse chromosome 17 and analysis of allelic expression of genes flanking the imprinted Igf2r gene. Genomics. 1997;43:285-97 pubmed
    ..The remaining genes in this region have previously been shown to be biallelically expressed. ..
  18. Nerurkar P, Schut H, Anderson L, Riggs C, Snyderwine E, Thorgeirsson S, et al. DNA adducts of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in colon, bladder, and kidney of congenic mice differing in Ah responsiveness and N-acetyltransferase genotype. Cancer Res. 1995;55:3043-9 pubmed
    ..5-fold increase in adducts but only in the Ah-responsive, rapid acetylator mice. In sum, these polymorphisms influenced the level of IQ-DNA adducts in the kidney, urinary bladder, and colon in complex ways. ..
  19. Toledo J, Garda H, Cabaleiro L, Cuellar A, Pellon Maison M, GONZALEZ BARO M, et al. Apolipoprotein A-I Helsinki promotes intracellular acyl-CoA cholesterol acyltransferase (ACAT) protein accumulation. Mol Cell Biochem. 2013;377:197-205 pubmed publisher
    ..deletion at the C-terminal region (?K226), lead to a marked increase (more than 10 times) in the intracellular ACAT1 protein level as detected by western blot analysis...
  20. Hans C, Zerfaoui M, Naura A, Catling A, Boulares A. Differential effects of PARP inhibition on vascular cell survival and ACAT-1 expression favouring atherosclerotic plaque stability. Cardiovasc Res. 2008;78:429-39 pubmed publisher
    ..cellular-toxic-free cholesterol, potentially through a down-regulation of acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) expression...
  21. Takakubo F, Dahl H. Analysis of pyruvate dehydrogenase expression in embryonic mouse brain: localization and developmental regulation. Brain Res Dev Brain Res. 1994;77:63-76 pubmed
    ..Increased PDH activity at the end of gestation is attributed to an increase in transcription. Our data to a large extent explain pathological presentations in PDH E1 alpha deficient patients with congenital brain disorders. ..
  22. Bryleva E, Rogers M, Chang C, Buen F, Harris B, Rousselet E, et al. ACAT1 gene ablation increases 24(S)-hydroxycholesterol content in the brain and ameliorates amyloid pathology in mice with AD. Proc Natl Acad Sci U S A. 2010;107:3081-6 pubmed publisher
    ..Acyl-CoA:cholesterol acyltransferases (ACAT1 and ACAT2) are two enzymes that convert free cholesterol to cholesteryl esters...
  23. Bao L, Li Y, Deng S, Landry D, Tabas I. Sitosterol-containing lipoproteins trigger free sterol-induced caspase-independent death in ACAT-competent macrophages. J Biol Chem. 2006;281:33635-49 pubmed
    ..death, however, requires dysfunction of the sterol esterifying enzyme acyl-coenzyme A-cholesterol acyltransferase (ACAT), which likely occurs slowly during lesion progression...
  24. Wang S, Shi W, Wang X, Velky L, Greenlee S, Wang M, et al. Mapping, genetic isolation, and characterization of genetic loci that determine resistance to atherosclerosis in C3H mice. Arterioscler Thromb Vasc Biol. 2007;27:2671-6 pubmed
    ..We have also determined that Tlr4 on the C3H ApoE(-/-) background does not contribute to early lesion development. *Ath29 is referred to as Ath22 in Su et al 2006. ..
  25. Shibuya Y, Niu Z, Bryleva E, Harris B, Murphy S, Kheirollah A, et al. Acyl-coenzyme A:cholesterol acyltransferase 1 blockage enhances autophagy in the neurons of triple transgenic Alzheimer's disease mouse and reduces human P301L-tau content at the presymptomatic stage. Neurobiol Aging. 2015;36:2248-2259 pubmed publisher
    ..molecule enzyme inhibitors or genetic inactivation of acyl-coenzyme A (Acyl-CoA):cholesterol acyltransferase 1 (ACAT1) diminished amyloidopathy and restored cognitive deficits...
  26. Tomita K, Teratani T, Suzuki T, Shimizu M, Sato H, Narimatsu K, et al. Acyl-CoA:cholesterol acyltransferase 1 mediates liver fibrosis by regulating free cholesterol accumulation in hepatic stellate cells. J Hepatol. 2014;61:98-106 pubmed publisher
    ..FC accumulation in hepatic stellate cells (HSCs) plays a role in progression of liver fibrosis, but the effect of ACAT1 on liver fibrosis has not been clarified...