Genomes and Genes
Gene Symbol: MKC7
- Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiaeDamian J Krysan
Division of Pediatric Infectious Disease, Department of Pediatrics and Communicable Diseases, University of Michigan Hospitals, Ann Arbor, Michigan 48109, USA
Eukaryot Cell 4:1364-74. 2005..Taken together, these data indicate that the yapsins play an important role in glucan homeostasis in S. cerevisiae and that yapsin homologues may play a similar role in the pathogenic yeasts C. albicans and C. glabrata...
- Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeastH Komano
Department of Biochemistry, Stanford University School of Medicine, CA 94305 5307, USA
Proc Natl Acad Sci U S A 92:10752-6. 1995The MKC7 gene was isolated as a multicopy suppressor of the cold-sensitive growth phenotype of a yeast kex2 mutant, which lacks the protease that cleaves pro-alpha-factor and other secretory proproteins at pairs of basic residues in a ..
- Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiaeEun Young Cho
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
J Biotechnol 149:1-7. 2010..The present data strongly indicate the involvement of other yapsin members besides Yps1p in the proteolysis of secretory recombinant proteins, particularly under high-density growth conditions...
- Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeastNadia Vadaie
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA
J Cell Biol 181:1073-81. 2008..We postulate that cleavage-dependent activation is a general feature of signaling mucins, which brings to light a novel regulatory aspect of this class of signaling adhesion molecule...
- Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiaeN Tomishige
Department of Biotechnology, University of Tokyo, Bunkyo ku, 113 8657 Tokyo, Japan
Mol Genet Genomics 269:562-73. 2003..A cell wall defect was also found in the kex2Delta mutant, which was suppressible by multiple copies of the MKC7 or YAP3 gene, both of which encode other GPI-anchored proteases...
- YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteinsElena Friedmann
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 277:35274-81. 2002..Yos9 binds directly to Gas1 and Mkc7 and accelerates Gas1 transport and processing in cells overexpressing YOS9...
- Purification and characterization of the yeast glycosylphosphatidylinositol-anchored, monobasic-specific aspartyl protease yapsin 2 (Mkc7p)H Komano
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
J Biol Chem 274:24431-7. 1999The Saccharomyces cerevisiae YPS2 (formerly MKC7) gene product is a glycosylphosphatidylinositol-linked aspartyl protease that functions as a yeast secretase...
- Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the Kex2 endoprotease in vacuolar acidificationY E Oluwatosin
Department of Biochemistry and Molecular Biology, SUNY Health Science Center at Syracuse, New York 13210, USA
Mol Cell Biol 18:1534-43. 1998..This is the first time a mutation of this type has been identified...
- Characterization of beta-amyloid peptide precursor processing by the yeast Yap3 and Mkc7 proteasesW Zhang
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314, USA
Biochim Biophys Acta 1359:110-22. 1997..Deletions of the genes encoding the GPI-linked aspartyl proteases Yap3 and Mkc7 decreased alpha-secretase activity by 56 and 29%, respectively; whereas, the double deletion decreased the activity ..
- Extracellular secretion of overexpressed glycosylphosphatidylinositol-linked cell wall protein Utr2/Crh2p as a novel protein quality control mechanism in Saccharomyces cerevisiaeKelly A Miller
Departments of Pediatrics and Microbiology Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Eukaryot Cell 9:1669-79. 2010..cerevisiae. This model provides an explanation for the deleterious effects of Yps1/2p on the industrial production of some recombinant proteins in S. cerevisiae...