pta

Summary

Gene Symbol: pta
Description: phosphate acetyltransferase
Alias: MA3607
Species: Methanosarcina acetivorans C2A

Top Publications

  1. Iyer P, Lawrence S, Luther K, Rajashankar K, Yennawar H, Ferry J, et al. Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila. Structure. 2004;12:559-67 pubmed publisher
    Phosphotransacetylase (Pta) [EC 2.3.1...
  2. Latimer M, Ferry J. Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila. J Bacteriol. 1993;175:6822-9 pubmed
    The genes for the acetate-activating enzymes, acetate kinase and phosphotransacetylase (ack and pta), from Methanosarcina thermophila TM-1 were cloned and sequenced...
  3. Rasche M, Smith K, Ferry J. Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 1997;179:7712-7 pubmed
    ..Five arginines were individually replaced with glutamine. Kinetic analysis of the altered enzymes identified R310 as essential for activity. Of the four nonessential for activity, R87 and R133 appear to be involved in binding CoA...
  4. Lawrence S, Luther K, Schindelin H, Ferry J. Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 2006;188:1143-54 pubmed publisher
    ..We propose that Arg310 binds acetyl phosphate and orients it for optimal nucleophilic attack. The hypothesized mechanism proceeds through a negatively charged transition state stabilized by hydrogen bond donation from Ser309...