MJ_RS06620

Summary

Gene Symbol: MJ_RS06620
Description: proline--tRNA ligase
Alias: MJ1238, MJ_1238
Species: Methanocaldococcus jannaschii DSM 2661

Top Publications

  1. Kamtekar S, Kennedy W, Wang J, Stathopoulos C, S ll D, Steitz T. The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases. Proc Natl Acad Sci U S A. 2003;100:1673-8 pubmed publisher
    ..jannaschii ProRS misacylates tRNA(Pro) with cysteine, and argue against the proposal that these archaeal ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cognate amino acids...
  2. Stathopoulos C, Li T, Longman R, Vothknecht U, Becker H, Ibba M, et al. One polypeptide with two aminoacyl-tRNA synthetase activities. Science. 2000;287:479-82 pubmed
    ..The ability of one enzyme to provide two aminoacyl-tRNAs for protein synthesis raises questions about concepts of substrate specificity in protein synthesis and may provide insights into the evolutionary origins of this process...
  3. Lipman R, Sowers K, Hou Y. Synthesis of cysteinyl-tRNA(Cys) by a genome that lacks the normal cysteine-tRNA synthetase. Biochemistry. 2000;39:7792-8 pubmed
    ..These considerations, among others, raised the distinct possibility that the M. jannaschii proline-tRNA synthetase may recruit additional protein or RNA factors to facilitate the synthesis of cysteinyl-tRNA(Cys)...
  4. Lipman R, Beuning P, Musier Forsyth K, Hou Y. Amino acid activation of a dual-specificity tRNA synthetase is independent of tRNA. J Mol Biol. 2002;316:421-7 pubmed
  5. Ahel I, Stathopoulos C, Ambrogelly A, Sauerwald A, Toogood H, Hartsch T, et al. Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetases. J Biol Chem. 2002;277:34743-8 pubmed publisher
    ..Therefore, ProRS exhibits a natural level of mischarging that is to date unequalled among the aminoacyl-tRNA synthetases...
  6. Ambrogelly A, Kamtekar S, Stathopoulos C, Kennedy D, Soll D. Asymmetric behavior of archaeal prolyl-tRNA synthetase. FEBS Lett. 2005;579:6017-22 pubmed
    ..Together with previous reports our results show that asymmetry might be a general feature of the aminoacylation reaction catalyzed by dimeric aminoacyl-tRNA synthetases from both classes...
  7. Stathopoulos C, Jacquin Becker C, Becker H, Li T, Ambrogelly A, Longman R, et al. Methanococcus jannaschii prolyl-cysteinyl-tRNA synthetase possesses overlapping amino acid binding sites. Biochemistry. 2001;40:46-52 pubmed
    ..The proximity in ProCysRS of amino acid residues affecting binding of either cysteine or proline strongly suggests that structural elements of the two amino acid binding sites overlap...
  8. Beuning P, Musier Forsyth K. Species-specific differences in amino acid editing by class II prolyl-tRNA synthetase. J Biol Chem. 2001;276:30779-85 pubmed publisher
    ..These results have implications for the selection or design of antibiotics that specifically target the editing active site of the prokaryotic-like group of ProRSs...
  9. Ambrogelly A, Ahel I, Polycarpo C, Bunjun Srihari S, Krett B, Jacquin Becker C, et al. Methanocaldococcus jannaschii prolyl-tRNA synthetase charges tRNA(Pro) with cysteine. J Biol Chem. 2002;277:34749-54 pubmed publisher
    ..We discuss the implications of these results on the in vivo activity of the M. jannaschii ProRS and on the nature of the enzyme involved in the synthesis of Cys-tRNA(Cys) in M. jannaschii...

More Information

Publications10

  1. Ruan B, Nakano H, Tanaka M, Mills J, DeVito J, Min B, et al. Cysteinyl-tRNA(Cys) formation in Methanocaldococcus jannaschii: the mechanism is still unknown. J Bacteriol. 2004;186:8-14 pubmed
    ..These results imply that it is not established that proS or MJ1477 gene products catalyze Cys-tRNA(Cys) synthesis in M. jannaschii. Thus, the mechanism of Cys-tRNA(Cys) formation in M. jannaschii still remains to be discovered...