N

Summary

Gene Symbol: N
Description: nucleocapsid protein
Species: Measles virus

Top Publications

  1. Bourhis J, Receveur Brechot V, Oglesbee M, Zhang X, Buccellato M, Darbon H, et al. The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. Protein Sci. 2005;14:1975-92 pubmed
    ..The measles virus nucleoprotein consists of a structured N-terminal domain, and of an intrinsically disordered C-terminal domain, N(TAIL) (aa 401-525), which undergoes induced ..
  2. Belle V, Rouger S, Costanzo S, Liquière E, Strancar J, Guigliarelli B, et al. Mapping alpha-helical induced folding within the intrinsically disordered C-terminal domain of the measles virus nucleoprotein by site-directed spin-labeling EPR spectroscopy. Proteins. 2008;73:973-88 pubmed publisher
    ..spectroscopy, we mapped the region of the intrinsically disordered C-terminal domain of measles virus nucleoprotein (N(TAIL)) that undergoes induced folding...
  3. Cattaneo R, Kaelin K, Baczko K, Billeter M. Measles virus editing provides an additional cysteine-rich protein. Cell. 1989;56:759-64 pubmed
    ..A smaller fraction of transcripts contain several additional G residues...
  4. Li H, Spencer S, Lian L, Zhang Z, Lu P. Phylogenetic analysis of the nucleoprotein gene of measles viruses prevalent in Nantong, Jiangsu Province, China, during 2010. Epidemiol Infect. 2012;140:1607-11 pubmed publisher
    ..Six isolates identified were all clustered within genotype H1. The findings reported here support continued endemic transmission of measles virus in China. ..
  5. Sugai A, Sato H, Yoneda M, Kai C. Phosphorylation of measles virus nucleoprotein affects viral growth by changing gene expression and genomic RNA stability. J Virol. 2013;87:11684-92 pubmed publisher
    The measles virus (MV) nucleoprotein associates with the viral RNA genome to form the N-RNA complex, providing a template for viral RNA synthesis...
  6. Iwasaki M, Takeda M, Shirogane Y, Nakatsu Y, Nakamura T, Yanagi Y. The matrix protein of measles virus regulates viral RNA synthesis and assembly by interacting with the nucleocapsid protein. J Virol. 2009;83:10374-83 pubmed publisher
    The genome of measles virus (MV) is encapsidated by the nucleocapsid (N) protein and associates with RNA-dependent RNA polymerase to form the ribonucleoprotein complex...
  7. Prodhomme E, Fack F, Revets D, Pirrotte P, Kremer J, Muller C. Extensive phosphorylation flanking the C-terminal functional domains of the measles virus nucleoprotein. J Proteome Res. 2010;9:5598-609 pubmed publisher
    ..Both recombinant NP (rNP) and vNP were ?-acetylated at the N-terminus...
  8. Longhi S. Nucleocapsid structure and function. Curr Top Microbiol Immunol. 2009;329:103-28 pubmed
    ..Its nonsegmented, single-stranded, negative-sense RNA genome is encapsidated by the nucleoprotein (N) to form a helical nucleocapsid...
  9. Shu Y, Habchi J, Costanzo S, Padilla A, Brunel J, Gerlier D, et al. Plasticity in structural and functional interactions between the phosphoprotein and nucleoprotein of measles virus. J Biol Chem. 2012;287:11951-67 pubmed publisher
    ..by the X domain of P (XD) and a conserved sequence (Box-2) within the C-terminal domain of the nucleoprotein (N(TAIL))...

More Information

Publications29

  1. Bellini W, Englund G, Richardson C, Rozenblatt S, Lazzarini R. Matrix genes of measles virus and canine distemper virus: cloning, nucleotide sequences, and deduced amino acid sequences. J Virol. 1986;58:408-16 pubmed
    ..Secondary-structure analyses of this shared region indicated a structurally conserved, hydrophobic sequence which possibly interacted with the lipid bilayer. ..
  2. Krumm S, Takeda M, Plemper R. The measles virus nucleocapsid protein tail domain is dispensable for viral polymerase recruitment and activity. J Biol Chem. 2013;288:29943-53 pubmed publisher
    Paramyxovirus genomes are ribonucleoprotein (RNP) complexes consisting of nucleoprotein (N)-encapsidated viral RNA...
  3. Han M, Xu J, Ren Y, Li J. Simulation of coupled folding and binding of an intrinsically disordered protein in explicit solvent with metadynamics. J Mol Graph Model. 2016;68:114-127 pubmed publisher
    ..With the advanced sampling approach, more IDP systems could be simulated and common mechanisms concerning the coupled folding and binding process could be investigated in the future. ..
  4. Bellini W, Englund G, Rozenblatt S, Arnheiter H, Richardson C. Measles virus P gene codes for two proteins. J Virol. 1985;53:908-19 pubmed
    ..Using immunofluorescent microscopy, we localized the C protein in the nucleus and in cytoplasmic inclusions within the infected cells...
  5. Takeuchi K, Miyajima N, Kobune F, Tashiro M. Comparative nucleotide sequence analyses of the entire genomes of B95a cell-isolated and vero cell-isolated measles viruses from the same patient. Virus Genes. 2000;20:253-7 pubmed
    ..These results indicated that nucleotide difference(s) in the P/V/C or/and M gene, and not H gene, was responsible for the different cell tropism and pathogenicity of MV in this case...
  6. Centeno R, Fuji N, Okamoto M, Dapat C, Saito M, Tandoc A, et al. Genetic characterization of measles virus in the Philippines, 2008-2011. BMC Res Notes. 2015;8:211 pubmed publisher
    ..detected in the Philippines from 2008 to 2011 were analyzed by sequencing the C-terminal region of nucleocapsid (N) gene and partial hemagglutinin (H) gene and by inferring the phylogenetic trees...
  7. Sugai A, Sato H, Hagiwara K, Kozuka Hata H, Oyama M, Yoneda M, et al. Newly identified minor phosphorylation site threonine-279 of measles virus nucleoprotein is a prerequisite for nucleocapsid formation. J Virol. 2014;88:1140-9 pubmed publisher
    ..Taken together, these results indicate that phosphorylation at T279 is a prerequisite for successful nucleocapsid formation. ..
  8. Takayama I, Sato H, Watanabe A, Omi Furutani M, Sugai A, Kanki K, et al. The nucleocapsid protein of measles virus blocks host interferon response. Virology. 2012;424:45-55 pubmed publisher
    ..Interestingly, we found that N protein acts as an IFN-?/? and ?-antagonist as strong as P gene products...
  9. Hagiwara K, Sato H, Inoue Y, Watanabe A, Yoneda M, Ikeda F, et al. Phosphorylation of measles virus nucleoprotein upregulates the transcriptional activity of minigenomic RNA. Proteomics. 2008;8:1871-9 pubmed publisher
    We report the first identification of phosphorylation sites of the nucleoprotein (N) of the family Paramyxoviridae...
  10. Sato H, Masuda M, Kanai M, Tsukiyama Kohara K, Yoneda M, Kai C. Measles virus N protein inhibits host translation by binding to eIF3-p40. J Virol. 2007;81:11569-76 pubmed publisher
    ..negative-sense RNA genome of measles virus (MV) is encapsidated by the virus-encoded nucleocapsid protein (N)...
  11. Brunel J, Chopy D, Dosnon M, Bloyet L, Devaux P, Urzua E, et al. Sequence of events in measles virus replication: role of phosphoprotein-nucleocapsid interactions. J Virol. 2014;88:10851-63 pubmed publisher
    ..of nonsegmented negative-strand RNA viruses is tightly embedded within a nucleocapsid made of a nucleoprotein (N) homopolymer...
  12. Sun Q, Sammut B, Wang F, Kurihara N, Windle J, Roodman G, et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res. 2014;29:90-102 pubmed publisher
    ..These results demonstrate that TBK1 plays a critical role in mediating the effects of MVNP on osteoclast differentiation and on the expression of IL-6, a key contributor to the pagetic osteoclast phenotype. ..
  13. Colombo M, Bourhis J, Chamontin C, Soriano C, Villet S, Costanzo S, et al. The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment. Virol J. 2009;6:59 pubmed publisher
    ..a non-segmented single-stranded RNA molecule of negative polarity, which is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid...
  14. Wardrop E, Briedis D. Characterization of V protein in measles virus-infected cells. J Virol. 1991;65:3421-8 pubmed
  15. Blocquel D, Habchi J, Costanzo S, Doizy A, Oglesbee M, Longhi S. Interaction between the C-terminal domains of measles virus nucleoprotein and phosphoprotein: a tight complex implying one binding site. Protein Sci. 2012;21:1577-85 pubmed publisher
    The intrinsically disordered C-terminal domain (N(TAIL) ) of the measles virus (MeV) nucleoprotein undergoes ?-helical folding upon binding to the C-terminal X domain (XD) of the phosphoprotein...
  16. Couturier M, Buccellato M, Costanzo S, Bourhis J, Shu Y, Nicaise M, et al. High affinity binding between Hsp70 and the C-terminal domain of the measles virus nucleoprotein requires an Hsp40 co-chaperone. J Mol Recognit. 2010;23:301-15 pubmed publisher
    ..Binding is mediated by two hydrophobic motifs (Box-2 and Box-3) located within the C-terminal domain (N(TAIL)) of the nucleocapsid protein, with N(TAIL) being an intrinsically disordered domain...
  17. Rima B, Duprex W. The measles virus replication cycle. Curr Top Microbiol Immunol. 2009;329:77-102 pubmed
  18. Wei C, Shi J, Liu B, Shi Y, Zheng J, Xu G, et al. Molecular characterization of the measles virus genotypes in JiLin Province, China. PLoS ONE. 2012;7:e46011 pubmed publisher
    ..The MV genotype H1 was the most prevalent strain. After sequencing the nucleoprotein (N) genes of these strains, a maximum clade credibility tree was constructed by the bayesian Markov Chain Monte Carlo ..
  19. Dosnon M, Bonetti D, Morrone A, Erales J, Di Silvio E, Longhi S, et al. Demonstration of a folding after binding mechanism in the recognition between the measles virus NTAIL and X domains. ACS Chem Biol. 2015;10:795-802 pubmed publisher
    ..These results contribute to enhancing our understanding of the molecular mechanisms by which IDPs recognize their partners and represent a paradigmatic example of the need of kinetic methods to discriminate between reaction mechanisms. ..
  20. Guryanov S, Liljeroos L, Kasaragod P, Kajander T, Butcher S. Crystal Structure of the Measles Virus Nucleoprotein Core in Complex with an N-Terminal Region of Phosphoprotein. J Virol. 2015;90:2849-57 pubmed publisher
    The enveloped negative-stranded RNA virus measles virus (MeV) is an important human pathogen. The nucleoprotein (N(0)) assembles with the viral RNA into helical ribonucleocapsids (NC) which are, in turn, coated by a helical layer of the ..