NP

Summary

Gene Symbol: NP
Description: nucleocapsid protein
Alias: nucleoprotein
Species: Marburg marburgvirus

Top Publications

  1. pmc Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
  2. ncbi Sequence analysis of the Marburg virus nucleoprotein gene: comparison to Ebola virus and other non-segmented negative-strand RNA viruses
    A Sanchez
    Department of Biology, Georgia State University, Atlanta 30302 4010
    J Gen Virol 73:347-57. 1992
  3. ncbi Rescue of Ebola virus from cDNA using heterologous support proteins
    Steven Theriault
    Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada
    Virus Res 106:43-50. 2004
  4. ncbi Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP-capture enzyme-linked immunosorbent assay
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 76:111-8. 2005
  5. ncbi Inhibition of Marburg virus protein expression and viral release by RNA interference
    Trent Fowler
    Max Planck Institute for Infection Biology, Schumannstrasse 21 22, Campus Charite Mitte, 10117 Berlin, Germany
    J Gen Virol 86:1181-8. 2005
  6. pmc Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcription
    Peggy Möller
    Institut für Virologie der Philipps Universität Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 79:14876-86. 2005
  7. pmc Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 80:1038-43. 2006
  8. pmc Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulence
    John C Kash
    Department of Microbiology, School of Medicine, University of Washington, Box 358070, Seattle, Washington 98195 8070, USA
    J Virol 80:3009-20. 2006
  9. pmc Functional mapping of the nucleoprotein of Ebola virus
    Shinji Watanabe
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, Wisconsin 53706, USA
    J Virol 80:3743-51. 2006
  10. ncbi Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 59:323-5. 2006

Scientific Experts

  • M Saijo
  • Heinz Feldmann
  • John C Kash
  • S Becker
  • Larissa Kolesnikova
  • Hans Dieter Klenk
  • E Muhlberger
  • Yoshihiro Kawaoka
  • Peggy Möller
  • Manos Mavrakis
  • Shuzo Urata
  • Takeshi Noda
  • Sven Enterlein
  • Shinji Watanabe
  • James L Murray
  • Trent Fowler
  • Steven Theriault
  • Yue Huang
  • H D Klenk
  • L Kolesnikova
  • Hideyoshi Yokosawa
  • Jiro Yasuda
  • Aparna B Bohil
  • Richard E Cheney
  • Shigeru Morikawa
  • Valentina Volchkova
  • M Hevey
  • Elke Mühlberger
  • Viktor Volchkov
  • Michael Weik
  • Thomas F Meyer
  • Natalie J McDonald
  • Jennifer Lippincott-Schwartz
  • Thomas W Hodge
  • Anthony Sanchez
  • William J Bellini
  • Thomas Rudel
  • Chi Cheng Luo
  • Lijun Zhao
  • Michael W Shaw
  • Jinsong Sheng
  • Mamadi Yilla
  • Nonia Pariente
  • Joseph M Le Doux
  • Sandra Bamberg
  • Donald H Rubin
  • Gabriele Neumann
  • Allison Groseth
  • Harald Bugany
  • Rob W H Ruigrok
  • Gary J Nabel
  • Guy Schoehn
  • Yongnian Sun
  • Ling Xu
  • E Ryabchikova
  • M Weik
  • V E Volchkov
  • D Negley
  • J Smith
  • P Pushko
  • A Schmaljohn
  • B Lötfering
  • A Sanchez
  • M P Kiley
  • C Will
  • A Randolf
  • N J Cox
  • J B McCormick
  • M J Buchmeier
  • R deFries
  • D D Richman
  • L H Elliott

Detail Information

Publications24

  1. pmc Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
    ..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...
  2. ncbi Sequence analysis of the Marburg virus nucleoprotein gene: comparison to Ebola virus and other non-segmented negative-strand RNA viruses
    A Sanchez
    Department of Biology, Georgia State University, Atlanta 30302 4010
    J Gen Virol 73:347-57. 1992
    ..in the sequence was a short putative leader sequence at the extreme 3' end, followed by the complete nucleoprotein (NP) gene. The 5' end of the NP mRNA was determined as was the polyadenylation site for the NP gene...
  3. ncbi Rescue of Ebola virus from cDNA using heterologous support proteins
    Steven Theriault
    Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada
    Virus Res 106:43-50. 2004
    ..Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps...
  4. ncbi Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP-capture enzyme-linked immunosorbent assay
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 76:111-8. 2005
    ..In order to establish a diagnostic system for Marburg hemorrhagic fever by the detection of Marburg virus nucleoprotein, monoclonal antibodies to the recombinant nucleoprotein were produced...
  5. ncbi Inhibition of Marburg virus protein expression and viral release by RNA interference
    Trent Fowler
    Max Planck Institute for Infection Biology, Schumannstrasse 21 22, Campus Charite Mitte, 10117 Berlin, Germany
    J Gen Virol 86:1181-8. 2005
    ..Small interfering RNAs (siRNAs) homologous to three MARV transcripts (NP, VP35 and VP30) were co-transfected into cells with plasmids encoding the corresponding nucleocapsid proteins...
  6. pmc Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcription
    Peggy Möller
    Institut für Virologie der Philipps Universität Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 79:14876-86. 2005
    ..In addition, a trimeric complex had been detected in which VP35 bridges L and the nucleoprotein NP. It has been presumed that the trimeric complex represents the active polymerase bound to the nucleocapsid...
  7. pmc Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30
    Sven Enterlein
    Department of Virology, Philipps University Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 80:1038-43. 2006
    ..In contrast, rescue of recombinant EBOV was not supported by EBOV VP30 containing a mutated Zn-binding domain...
  8. pmc Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulence
    John C Kash
    Department of Microbiology, School of Medicine, University of Washington, Box 358070, Seattle, Washington 98195 8070, USA
    J Virol 80:3009-20. 2006
    ..Taken together, these studies showed a correlation between antagonism of type I IFN responses and filovirus virulence...
  9. pmc Functional mapping of the nucleoprotein of Ebola virus
    Shinji Watanabe
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, Wisconsin 53706, USA
    J Virol 80:3743-51. 2006
    At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. Huang et al. (Y...
  10. ncbi Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 59:323-5. 2006
    ..using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005)...
  11. ncbi Budding of Marburgvirus is associated with filopodia
    Larissa Kolesnikova
    Institute of Virology, Marburg Philipps University, Marburg, Germany
    Cell Microbiol 9:939-51. 2007
    ..As filopodia are in close contact with neighbouring cells, usurpation of these structures may facilitate spread of MARV to adjacent cells...
  12. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
    ..Expression of the nucleoprotein (NP) and virion-associated proteins VP35 and VP24 led to spontaneous assembly of nucleocapsids in transfected ..
  13. ncbi Morphology of Marburg virus NP-RNA
    Manos Mavrakis
    EMBL Grenoble Outstation, Grenoble Cedex 9, France
    Virology 296:300-7. 2002
    When Marburg virus (MBGV) nucleoprotein (NP) is expressed in insect cells, it binds to cellular RNA and forms NP-RNA complexes such as insect cell-expressed nucleoproteins from other nonsegmented negative-strand RNA viruses...
  14. ncbi The nucleotide sequence of the L gene of Marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses
    E Muhlberger
    Institut fuer Virologie, Philipps Universitaet, Marburg, Germany
    Virology 187:534-47. 1992
    ..The data presented here suggest a common evolutionary history for all nonsegmented negative-stranded RNA viruses and show that filoviruses are more closely related to paramyxo- than to rhabdoviruses...
  15. ncbi Physicochemical properties of Marburg virus: evidence for three distinct virus strains and their relationship to Ebola virus
    M P Kiley
    Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333
    J Gen Virol 69:1957-67. 1988
    ..Peptide mapping of glycoproteins, nucleoproteins (NP) and viral structural protein (VP40) demonstrated extensive sequence conservation in the proteins of viruses ..
  16. ncbi The nucleoprotein of Marburg virus is phosphorylated
    S Becker
    Institut fur Virologie, Marburg, Germany
    J Gen Virol 75:809-18. 1994
    The nucleoprotein (NP) of Marburg virus (MBG), a filovirus, is encoded by the gene closest to the 3' end of the non-segmented negative-strand RNA genome...
  17. pmc Three of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomes
    E Muhlberger
    Institut fur Virologie, Philipps Universitat Marburg, 35037 Marburg, Germany
    J Virol 72:8756-64. 1998
    ..of the order Mononegavirales, filoviruses possess four proteins presumed to be components of the nucleocapsid (NP, VP35, VP30, and L)...
  18. ncbi Interactions of Marburg virus nucleocapsid proteins
    S Becker
    Institut für Virologie der Philipps Universität Marburg, Robert Koch str 17, Marburg, 35037, Germany
    Virology 249:406-17. 1998
    ..Using salt dissociation of isolated virions, four proteins (NP, VP35, VP30, and L) remained attached to the core complex...
  19. ncbi Marburg virus vaccines based upon alphavirus replicons protect guinea pigs and nonhuman primates
    M Hevey
    United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, Frederick, Maryland, 21702, USA
    Virology 251:28-37. 1998
    ..VEE) virus, was used as a vaccine vector; the VEE structural genes were replaced by genes for MBGV GP, nucleoprotein (NP), VP40, VP35, VP30, or VP24...
  20. pmc Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems
    E Muhlberger
    Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
    J Virol 73:2333-42. 1999
    ..As it was shown previously for MBGV, three of the four EBOV nucleocapsid proteins, NP, VP35, and L, were essential and sufficient for replication...
  21. pmc Ultrastructural organization of recombinant Marburg virus nucleoprotein: comparison with Marburg virus inclusions
    L Kolesnikova
    State Scientific Research Center of Virology, Vector Institute of Molecular Biology, Laboratory of Ultrastructure, 633159 Koltsovo, Novosibirsk Region, Russia
    J Virol 74:3899-904. 2000
    HeLa cells expressing the recombinant Marburg virus (MBGV) nucleoprotein (NP) have been studied by immunoelectron microscopy...
  22. ncbi Biosynthesis and role of filoviral glycoproteins
    H Feldmann
    Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2
    J Gen Virol 82:2839-48. 2001
  23. pmc VP40, the matrix protein of Marburg virus, is associated with membranes of the late endosomal compartment
    Larissa Kolesnikova
    Institut für Virologie der Philipps Universität Marburg, D 35037 Marburg, Germany
    J Virol 76:1825-38. 2002
    ..Our data indicate that MBGV VP40 was able to interact with membranes of late endosomes in the course of viral infection. This capability was independent of other MBGV proteins...
  24. pmc Interaction of Tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and Tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP
    Shuzo Urata
    First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
    J Virol 81:4895-9. 2007
    ..motif is important for VP40-induced VLP budding and that VLP production is significantly enhanced by coexpression of NP and GP...