Gene Symbol: NP
Description: nucleocapsid protein
Species: Marburg marburgvirus
- Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virusJames L Murray
National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Virol 79:11742-51. 2005..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...
- Sequence analysis of the Marburg virus nucleoprotein gene: comparison to Ebola virus and other non-segmented negative-strand RNA virusesA Sanchez
Department of Biology, Georgia State University, Atlanta 30302 4010
J Gen Virol 73:347-57. 1992..in the sequence was a short putative leader sequence at the extreme 3' end, followed by the complete nucleoprotein (NP) gene. The 5' end of the NP mRNA was determined as was the polyadenylation site for the NP gene...
- Rescue of Ebola virus from cDNA using heterologous support proteinsSteven Theriault
Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada
Virus Res 106:43-50. 2004..Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps...
- Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP-capture enzyme-linked immunosorbent assayMasayuki Saijo
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
J Med Virol 76:111-8. 2005..In order to establish a diagnostic system for Marburg hemorrhagic fever by the detection of Marburg virus nucleoprotein, monoclonal antibodies to the recombinant nucleoprotein were produced...
- Inhibition of Marburg virus protein expression and viral release by RNA interferenceTrent Fowler
Max Planck Institute for Infection Biology, Schumannstrasse 21 22, Campus Charite Mitte, 10117 Berlin, Germany
J Gen Virol 86:1181-8. 2005..Small interfering RNAs (siRNAs) homologous to three MARV transcripts (NP, VP35 and VP30) were co-transfected into cells with plasmids encoding the corresponding nucleocapsid proteins...
- Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcriptionPeggy Möller
Institut für Virologie der Philipps Universität Marburg, Robert Koch str 17, 35037 Marburg, Germany
J Virol 79:14876-86. 2005..In addition, a trimeric complex had been detected in which VP35 bridges L and the nucleoprotein NP. It has been presumed that the trimeric complex represents the active polymerase bound to the nucleocapsid...
- Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30Sven Enterlein
Department of Virology, Philipps University Marburg, Robert Koch str 17, 35037 Marburg, Germany
J Virol 80:1038-43. 2006..In contrast, rescue of recombinant EBOV was not supported by EBOV VP30 containing a mutated Zn-binding domain...
- Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulenceJohn C Kash
Department of Microbiology, School of Medicine, University of Washington, Box 358070, Seattle, Washington 98195 8070, USA
J Virol 80:3009-20. 2006..Taken together, these studies showed a correlation between antagonism of type I IFN responses and filovirus virulence...
- Functional mapping of the nucleoprotein of Ebola virusShinji Watanabe
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, Wisconsin 53706, USA
J Virol 80:3743-51. 2006At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. Huang et al. (Y...
- Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic MarburgvirusMasayuki Saijo
Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
Jpn J Infect Dis 59:323-5. 2006..using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005)...
- Budding of Marburgvirus is associated with filopodiaLarissa Kolesnikova
Institute of Virology, Marburg Philipps University, Marburg, Germany
Cell Microbiol 9:939-51. 2007..As filopodia are in close contact with neighbouring cells, usurpation of these structures may facilitate spread of MARV to adjacent cells...
- The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoproteinYue Huang
Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 10:307-16. 2002..Expression of the nucleoprotein (NP) and virion-associated proteins VP35 and VP24 led to spontaneous assembly of nucleocapsids in transfected ..
- Morphology of Marburg virus NP-RNAManos Mavrakis
EMBL Grenoble Outstation, Grenoble Cedex 9, France
Virology 296:300-7. 2002When Marburg virus (MBGV) nucleoprotein (NP) is expressed in insect cells, it binds to cellular RNA and forms NP-RNA complexes such as insect cell-expressed nucleoproteins from other nonsegmented negative-strand RNA viruses...
- The nucleotide sequence of the L gene of Marburg virus, a filovirus: homologies with paramyxoviruses and rhabdovirusesE Muhlberger
Institut fuer Virologie, Philipps Universitaet, Marburg, Germany
Virology 187:534-47. 1992..The data presented here suggest a common evolutionary history for all nonsegmented negative-stranded RNA viruses and show that filoviruses are more closely related to paramyxo- than to rhabdoviruses...
- Physicochemical properties of Marburg virus: evidence for three distinct virus strains and their relationship to Ebola virusM P Kiley
Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333
J Gen Virol 69:1957-67. 1988..Peptide mapping of glycoproteins, nucleoproteins (NP) and viral structural protein (VP40) demonstrated extensive sequence conservation in the proteins of viruses ..
- The nucleoprotein of Marburg virus is phosphorylatedS Becker
Institut fur Virologie, Marburg, Germany
J Gen Virol 75:809-18. 1994The nucleoprotein (NP) of Marburg virus (MBG), a filovirus, is encoded by the gene closest to the 3' end of the non-segmented negative-strand RNA genome...
- Three of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomesE Muhlberger
Institut fur Virologie, Philipps Universitat Marburg, 35037 Marburg, Germany
J Virol 72:8756-64. 1998..of the order Mononegavirales, filoviruses possess four proteins presumed to be components of the nucleocapsid (NP, VP35, VP30, and L)...
- Interactions of Marburg virus nucleocapsid proteinsS Becker
Institut für Virologie der Philipps Universität Marburg, Robert Koch str 17, Marburg, 35037, Germany
Virology 249:406-17. 1998..Using salt dissociation of isolated virions, four proteins (NP, VP35, VP30, and L) remained attached to the core complex...
- Marburg virus vaccines based upon alphavirus replicons protect guinea pigs and nonhuman primatesM Hevey
United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, Frederick, Maryland, 21702, USA
Virology 251:28-37. 1998..VEE) virus, was used as a vaccine vector; the VEE structural genes were replaced by genes for MBGV GP, nucleoprotein (NP), VP40, VP35, VP30, or VP24...
- Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systemsE Muhlberger
Institut für Virologie der Philipps Universität Marburg, 35037 Marburg, Germany
J Virol 73:2333-42. 1999..As it was shown previously for MBGV, three of the four EBOV nucleocapsid proteins, NP, VP35, and L, were essential and sufficient for replication...
- Ultrastructural organization of recombinant Marburg virus nucleoprotein: comparison with Marburg virus inclusionsL Kolesnikova
State Scientific Research Center of Virology, Vector Institute of Molecular Biology, Laboratory of Ultrastructure, 633159 Koltsovo, Novosibirsk Region, Russia
J Virol 74:3899-904. 2000HeLa cells expressing the recombinant Marburg virus (MBGV) nucleoprotein (NP) have been studied by immunoelectron microscopy...
- Biosynthesis and role of filoviral glycoproteinsH Feldmann
Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2
J Gen Virol 82:2839-48. 2001
- VP40, the matrix protein of Marburg virus, is associated with membranes of the late endosomal compartmentLarissa Kolesnikova
Institut für Virologie der Philipps Universität Marburg, D 35037 Marburg, Germany
J Virol 76:1825-38. 2002..Our data indicate that MBGV VP40 was able to interact with membranes of late endosomes in the course of viral infection. This capability was independent of other MBGV proteins...
- Interaction of Tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and Tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GPShuzo Urata
First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
J Virol 81:4895-9. 2007..motif is important for VP40-induced VLP budding and that VLP production is significantly enhanced by coexpression of NP and GP...