Genomes and Genes
Gene Symbol: KEM1
Alias: DST2, RAR5, SEP1, SKI1, XRN1
- A role for KEM1 at the START of the cell cycle in Saccharomyces cerevisiaeRitu Pathak
Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, TX 77843, USA
Curr Genet 48:300-9. 2005b>KEM1 is a Saccharomyces cerevisiae gene, conserved in all eukaryotes, whose deletion leads to pleiotropic phenotypes...
- A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiaeJulia Berretta
Centre de Genetique Moleculaire Centre National de la Recherche Scientifique CGM CNRS, 91198 Gif Yvette, France
Genes Dev 22:615-26. 2008..Our results show the first example of an RNA-dependent gene trans-silencing mediated by epigenetic marks in S. cerevisiae...
- Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processesVicky Goler-Baron
Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, Haifa 31096, Israel
Genes Dev 22:2022-7. 2008..Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay...
- A Sm-like protein complex that participates in mRNA degradationE Bouveret
EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
EMBO J 19:1661-71. 2000..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming...
- Decapping and decay of messenger RNA occur in cytoplasmic processing bodiesUjwal Sheth
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
Science 300:805-8. 2003..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
- The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complexJ S Anderson
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
EMBO J 17:1497-506. 1998..These observations argue that efficient mRNA turnover is required for viability and that we have identified the two major pathways of mRNA decay in yeast...
- The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1Nicole Fischer
Department of Molecular and Cell Biology, Division of Cell and Developmental Biology, University of California Berkeley, Berkeley, CA 94720 3200, USA
EMBO J 21:2788-97. 2002..and is shown to be in a complex with the mRNA degradation factor Pat1/Mtr1 and with the 5'-3' exoribonuclease Xrn1. Dhh1 specifically affects mRNA turnover in the deadenylation-dependent decay pathway, but does not act on the ..
- An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexesMasato Taoka
Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Minamiosawa 1 1, Hachioji shi, Tokyo 192 0397, Japan
Nucleic Acids Res 37:e140. 2009....
- A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decaySarah E Cole
Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
Mol Cell 34:440-50. 2009..We propose that 18S NRD and NGD are different observable outcomes of the same initiating event: a ribosome stalled inappropriately at a sense codon during translation elongation...
- Analysis of Dom34 and its function in no-go decayDario O Passos
University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, AZ 85721, USA
Mol Biol Cell 20:3025-32. 2009..We also provide evidence that the process of NGD is conserved in insect cells. On the basis of these results and the process of translation termination, we suggest a multistep model for the process of NGD...
- H3 lysine 4 di- and tri-methylation deposited by cryptic transcription attenuates promoter activationMarina Pinskaya
Centre de Genetique Moleculaire CNRS, Universite Pierre et Marie Curie, Gif sur Yvette, France
EMBO J 28:1697-707. 2009..Our data support a model wherein certain promoters are embedded in a repressive chromatin controlled by cryptic transcription...
- The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosomeClaudia Schneider
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
Nucleic Acids Res 37:1127-40. 2009..In BY4741, rrp44-exo was synthetic-lethal with loss of the cytoplasmic 5'-exonuclease Xrn1, indicating block of mRNA turnover, but not with loss of the nuclear 3'-exonuclease Rrp6...
- 20S RNA narnavirus defies the antiviral activity of SKI1/XRN1 in Saccharomyces cerevisiaeRosa Esteban
Instituto de Microbiologia Bioquimica, Departamento de Microbiologia y Genetica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Salamanca 37007, Spain
J Biol Chem 283:25812-20. 2008..Here we present evidence that the SKI1/XRN1 5'-exonuclease plays a major role in the elimination of the non-viral upstream sequences from the primary ..
- Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1Irina Chernyakov
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
Genes Dev 22:1369-80. 2008..We show here that components of this RTD pathway include the 5'-3' exonucleases Rat1 and Xrn1, and Met22, which likely acts indirectly through Rat1 and Xrn1...
- The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domainLin Chun Chang
Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan
Nucleic Acids Res 40:1331-44. 2012..Thus, we propose that the non-conserved C-terminus of Dhh1p plays a role in defining specific interactions with mRNA regulatory factors that promote distinct mRNA decay...
- Yeast RNase III triggers polyadenylation-independent transcription terminationGhada Ghazal
RNA Group, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Mol Cell 36:99-109. 2009..These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals...
- P-body components are required for Ty1 retrotransposition during assembly of retrotransposition-competent virus-like particlesMary Ann Checkley
Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute Frederick, P O Box B, Frederick, MD 21702 1201, USA
Mol Cell Biol 30:382-98. 2010..We have identified cytoplasmic P-body components encoded by DHH1, KEM1, LSM1, and PAT1 as cofactors that posttranscriptionally enhance Ty1 retrotransposition...
- Insights into the role of yeast eIF2A in IRES-mediated translationLucas C Reineke
Department of Biochemistry, Case Western Reserve University, School of Medicine, Cleveland, Ohio, United States of America
PLoS ONE 6:e24492. 2011..These data suggest that eIF2A acts as a switch to regulate IRES-mediated translation, and eEF1A may be an important mediator of translational activation during ethanol stress...
- Cryptic transcription mediates repression of subtelomeric metal homeostasis genesIsabelle Toesca
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America
PLoS Genet 7:e1002163. 2011....
- XUTs are a class of Xrn1-sensitive antisense regulatory non-coding RNA in yeastE L van Dijk
Centre de Génétique Moléculaire CNRS UPR 3404, Avenue de la Terrasse, 91198 Gif sur Yvette, France
Nature 475:114-7. 2011..In Saccharomyces cerevisiae, the bona-fide regulatory ncRNAs are destabilized by the Xrn1 5'-3' RNA exonuclease (also known as Kem1), but the genome-wide characterization of the entire regulatory ncRNA ..
- The oxidative stress response in yeast cells involves changes in the stability of Aft1 regulon mRNAsLaia Castells-Roca
Departament de Ciencies Mediques Basiques, IRBLLEIDA, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
Mol Microbiol 81:232-48. 2011..cerevisiae cells redirect iron assimilation through the non-reductive pathway to minimize oxidative damage by the ferrous ions, which are formed during iron import through the Ftr1/Fet3 complexes...
- The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1Cornelia Kurischko
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
J Cell Biol 192:583-98. 2011....
- Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeastGuangzuo Luo
Department of Molecular and Cellular Biochemistry and the Lucille Markey Cancer Center, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA
J Biol Chem 286:8759-70. 2011..Mammals may use similar regulatory mechanisms because components of the decay apparatus and signaling pathways are conserved...
- Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeastNaoko Mauchi
Department of Molecular Cell Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tennoudai, Tsukuba, Japan
Cell Struct Funct 35:95-105. 2010..However, mutations in DCP1 and DCP2, encoding a decapping enzyme complex, and XRN1, encoding a 5'-3' exonuclease, restored the decreased MTL1 mRNA levels...
- Decapping activators in Saccharomyces cerevisiae act by multiple mechanismsTracy Nissan
Department of Molecular Biology, Umea University, SE 901 87 Umea, Sweden
Mol Cell 39:773-83. 2010..We also identify numerous direct interactions between Pat1, Dcp1, Dcp2, Dhh1, Scd6, Edc3, Xrn1, and the Lsm1-7 complex...
- A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolismBernardo Cuenca-Bono
mRNA Transport Lab, Centro de Investigacion Principe Felipe, E 46013, Valencia, Spain
BMC Cell Biol 11:19. 2010..Through these associations, Sus1 mediates the nuclear dynamics of different gene loci and facilitate the export of the new transcripts...
- 5' to 3' mRNA decay factors colocalize with Ty1 gag and human APOBEC3G and promote Ty1 retrotranspositionJames A Dutko
Laboratory of Molecular Genetics, Wadsworth Center, University at Albany, Albany, NY 12201 2002, USA
J Virol 84:5052-66. 2010..of DDD and NMD explain the strong requirement for general 5' to 3' mRNA degradation factors Dcp1, Dcp2, and Xrn1 in Ty1 retromobility...
- The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase IISilvia Jimeno-González
Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus DK 8000, Denmark
Mol Cell 37:580-7. 2010..Our data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus...
- The DEAD-box RNA helicase Ded1p affects and accumulates in Saccharomyces cerevisiae P-bodiesCarla Beckham
Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85721 0206, USA
Mol Biol Cell 19:984-93. 2008..Combined with earlier work showing Ded1p can have a positive effect on translation, these results suggest that Ded1p is a bifunctional protein that can affect both translation initiation and P-body formation...
- Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decayBrian Luke
Swiss Institute for Experimental Cancer Research ISREC, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
Nucleic Acids Res 35:7688-97. 2007..Overall our findings suggest that NMD is more conserved in evolution than previously thought, and that at least one of the Smg5-7 proteins is conserved in budding yeast...
- The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanismsRona Lotan
Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
J Cell Biol 178:1133-43. 2007..Our genetic analyses suggest that Rpb7p plays two distinct roles in mRNA decay, which can both be uncoupled from Rpb7p's role in transcription. Thus, Rpb7p plays pivotal roles in determining mRNA levels...
- NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiaeJ H Ho
Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas, USA
Mol Cell Biol 19:2389-99. 1999A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover...
- Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzymeB Schwer
Microbiology Department, Cornell University Medical College, New York and Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
Nucleic Acids Res 26:2050-7. 1998..Uncapped poly(A)+ SSA4 mRNA accumulated in cells lacking the 5' exoribonuclease Xrn1. These findings provide genetic evidence for the long-held idea that the cap guanylate is critical for mRNA ..
- Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectivelyA W Johnson
Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712 1095, USA
Mol Cell Biol 17:6122-30. 1997b>XRN1 encodes an abundant cytoplasmic exoribonuclease, Xrn1p, responsible for mRNA turnover in yeast...
- Nuclear fusion in the yeast Saccharomyces cerevisiaeM D Rose
Department of Molecular Biology, Princeton University, New Jersey 08544 1014, USA
Annu Rev Cell Dev Biol 12:663-95. 1996..Accordingly, yeast karyogamy has become an important model system to investigate critical functions of the cytoplasmic microtubules and the microtubule organizing center, the nuclear envelope, and the endoplasmic reticulum...
- ROK1, a high-copy-number plasmid suppressor of kem1, encodes a putative ATP-dependent RNA helicase in Saccharomyces cerevisiaeY Song
Department of Microbiology, College of Natural Sciences, Chungnam National University, Daejeon, South Korea
Gene 166:151-4. 1995..KEM1 was also independently identified as DST2, SEP1, XRN1 and RAR5 on the basis of DNA strand transferase or exoribonuclease activity in vitro or mutations ..
- Synthetic lethality of sep1 (xrn1) ski2 and sep1 (xrn1) ski3 mutants of Saccharomyces cerevisiae is independent of killer virus and suggests a general role for these genes in translation controlA W Johnson
Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Boston, Massachusetts, USA
Mol Cell Biol 15:2719-27. 1995Strand exchange protein 1 (Sep1) (also referred to as exoribonuclease I [Xrn1]) from Saccharomyces cerevisiae has been implicated in DNA recombination, RNA turnover, karyogamy, and G4 DNA pairing among other disparate cellular processes...
- A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiaeH Interthal
Institute of General Microbiology, University of Bern, Switzerland
EMBO J 14:1057-66. 1995Saccharomyces cerevisiae cells lacking the SEP1 (also known as XRN1, KEM1, DST2, RAR5) gene function exhibit a number of phenotypes in cellular processes related to microtubule function...
- The sep1 mutant of Saccharomyces cerevisiae arrests in pachytene and is deficient in meiotic recombinationD X Tishkoff
Dana Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genetics 139:495-509. 1995Strand exchange protein 1 (Sep1) from Saccharomyces cerevisiae promotes homologous pairing of DNA in vitro and sep1 mutants display pleiotropic phenotypes in both vegetative and meiotic cells...
- "Superkiller" mutations suppress chromosomal mutations affecting double-stranded RNA killer plasmid replication in saccharomyces cerevisiaeA Toh-e
Proc Natl Acad Sci U S A 77:527-30. 1980..The ski1-1 mutation suppresses (bypasses) all mak mutations tested except mak16-1...
- kem mutations affect nuclear fusion in Saccharomyces cerevisiaeJ Kim
Massachusetts Institute of Technology, Cambridge
Genetics 126:799-812. 1990We have identified mutations in three genes of Saccharomyces cerevisiae, KEM1, KEM2 and KEM3, that enhance the nuclear fusion defect of kar1-1 yeast during conjugation. The KEM1 and KEM3 genes are located on the left arm of chromosome VII...
- The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiaeL Benard
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
J Virol 73:2893-900. 1999..b>ski1/xrn1Delta ski2Delta and ski1/xrn1Delta ski7Delta mutants were viable but temperature sensitive for growth.
- KEM1 is involved in filamentous growth of Saccharomyces cerevisiaeJaehee Kim
Department of Microbiology, College of Natural Sciences, Chungnam National University, Youseong Gu, Gung Dong, Taejeon, South Korea
FEMS Microbiol Lett 216:33-8. 2002The KEM1/XRN1 gene was originally identified because of its functions in microtubule-mediated processes, and is also known to be a major cytoplasmic 5'-3' exoribonuclease gene, which is involved in RNA turnover...
- Identification of protein complexes required for efficient sister chromatid cohesionMelanie L Mayer
Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4
Mol Biol Cell 15:1736-45. 2004..Furthermore, we find that genes involved in mitotic spindle integrity and positioning have a previously unrecognized role in sister chromatid cohesion...
- The yeast ubiquitin protease, Ubp3p, promotes protein stabilityChristine T Brew
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Genetics 162:1079-89. 2002..show that the temperature-sensitive stu1-5 allele is synthetically lethal in combination with ubp3, gim1-gim5, and kem1 mutations. The primary focus of this article is on the stu1-5 ubp3 interaction...
- Suppression of viral RNA recombination by a host exoribonucleaseChi Ping Cheng
Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, Kentucky 40546, USA
J Virol 80:2631-40. 2006..A previous genome-wide screen in Saccharomyces cerevisiae, a model host, identified five host genes, including XRN1, encoding a 5'-3' exoribonuclease, whose absence led to an approximately 10- to 50-fold enhancement of RNA ..
- Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homologYoung Un Park
Department of Microbiology, College of Natural Sciences, Chungnam National University, Taejeon 305 764, Republic of Korea
Biochem Biophys Res Commun 293:733-40. 2002..identified by its ability on a high-copy number plasmid to suppress the nuclear fusion defect caused by the kem1 null mutation...
- Identification of a regulated pathway for nuclear pre-mRNA turnoverC Bousquet-Antonelli
Wellcome Trust Centre for Cell Biology ICMB, University of Edinburgh, Scotland
Cell 102:765-75. 2000..We propose that nuclear pre-mRNA turnover represents a novel step in the regulation of gene expression...
- Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1pY Xue
Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712 1095, USA
Mol Cell Biol 20:4006-15. 2000..is synthetically lethal with the rat1-1(ts) mutation and shows genetic interaction with a deletion of SKI2 but not XRN1. Polysome analysis of an rai1 deletion mutant indicated a defect in 60S biogenesis which was nearly fully reversed ..
- Inhibition of mRNA turnover in yeast by an xrn1 mutation enhances the requirement for eIF4E binding to eIF4G and for proper capping of transcripts by Ceg1pJ T Brown
Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712 1095, USA
Genetics 155:31-42. 2000Null mutants of XRN1, encoding the major cytoplasmic exoribonuclease in yeast, are viable but accumulate decapped, deadenylated transcripts...
- The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivoJ T Brown
Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, 78712 1095, USA
RNA 6:449-57. 2000..XRN1 (SKI1) encodes a cytoplasmic 5'-exoribonuclease responsible for the majority of cytoplasmic RNA turnover, whereas SKI2, ..
- Ribosome synthesis in Saccharomyces cerevisiaeJ Venema
Department of Biochemistry and Molecular Biology, BioCentrum Amsterdam, Vrije Universiteit, The Netherlands
Annu Rev Genet 33:261-311. 1999..Here, we will discuss the recent, and often surprising, advances in our understanding of ribosome synthesis in the yeast Saccharomyces cerevisiae. These will underscore the unexpected complexity of eukaryotic ribosome synthesis...
- Chromosomal superkiller mutants of Saccharomyces cerevisiaeA Toh-e
J Bacteriol 136:1002-7. 1978..e., increased secretion of killer toxin activity by strains carrying the killer genome. These genes are designated ski1 through ski4 (for superkiller), ski3 and ski4 are located on chromosome XIV, and ski1 is on chromosome VII...
- Export and Maturation of Ribosomal SubunitsArlen W Johnson; Fiscal Year: 2010..Understanding these pathways and how they are integrated with other cellular pathways will provide the intellectual underpinning for investigators carrying out translational research. ..
- MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEASTArlen Johnson; Fiscal Year: 2004..Two models accounting for these results are that this complex is required for normal 60S function or that this complex acts in a 3'-mRNA degradation pathway. Experiments will be carried out to distinguish between these two models. ..
- MRNA DEGRADATION AND TRANSLATION CONTROL IN YEASTArlen Johnson; Fiscal Year: 1999..cerevisiae as a model system. The focus is on the XRN1 gene, encoding the major 5'-3' exoribonuclease activity of yeast and believed to be the major catabolic enzyme in ..
- MATURATION OF THE LARGE RIBOSOMAL SUBUNIT IN YEASTArlen Johnson; Fiscal Year: 2009..3) Determine the function of Arx1 p and Reilp, two functionally related proteins that act at the polypeptide exit tunnel on the nascent large ribosomal subunit during export and as it enters the cytoplasmic pool of active subunits. ..