flavivirus polyprotein gene


Gene Symbol: flavivirus polyprotein gene
Description: JEV polyprotein
Alias: JEV polyprotein, flavivirus polyprotein
Species: Japanese encephalitis virus
Products:     flavivirus polyprotein gene

Top Publications

  1. Luca V, Abimansour J, Nelson C, Fremont D. Crystal structure of the Japanese encephalitis virus envelope protein. J Virol. 2012;86:2337-46 pubmed publisher
    ..Our results suggest that variation in dimer structure and stability may significantly influence the assembly, receptor interaction, and uncoating of virions. ..
  2. Chiou C, Hu C, Chen P, Liao C, Lin Y, Wang J. Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein. J Gen Virol. 2003;84:2795-805 pubmed
    ..Our observations indicate that microtubules and TSG101 associate with NS3, which is incorporated into the JEV-induced structure during JEV replication. ..
  3. Sumiyoshi H, Mori C, Fuke I, Morita K, Kuhara S, Kondou J, et al. Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology. 1987;161:497-510 pubmed
    ..The C-terminal region of the ORF may encode a RNA-dependent RNA polymerase which has significant sequence homology with those of other RNA viruses. ..
  4. Kim J, Yun S, Song B, Hahn Y, Lee C, Oh H, et al. A single N-linked glycosylation site in the Japanese encephalitis virus prM protein is critical for cell type-specific prM protein biogenesis, virus particle release, and pathogenicity in mice. J Virol. 2008;82:7846-62 pubmed publisher
    ..Overall, our findings indicate that this highly conserved N-glycosylation motif in prM is crucial for multiple stages of JEV biology: prM biogenesis, virus release, and pathogenesis. ..
  5. Lu G, Gong P. Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface. PLoS Pathog. 2013;9:e1003549 pubmed publisher
    ..Our work paves the way for further dissection of the inter-regulations of the essential enzymatic activities of NS5 and exploration of possible other conformations of NS5 under different circumstances. ..
  6. Firth A, Atkins J. A conserved predicted pseudoknot in the NS2A-encoding sequence of West Nile and Japanese encephalitis flaviviruses suggests NS1' may derive from ribosomal frameshifting. Virol J. 2009;6:14 pubmed publisher
    ..We hypothesize that this may explain the origin of the previously identified NS1 'extension' protein in JEV-group flaviviruses, known as NS1'. ..
  7. Yang T, Shiu S, Chuang P, Lin Y, Wan L, Lan Y, et al. Japanese encephalitis virus NS2B-NS3 protease induces caspase 3 activation and mitochondria-mediated apoptosis in human medulloblastoma cells. Virus Res. 2009;143:77-85 pubmed publisher
  8. Ye Q, Li X, Zhao H, Li S, Deng Y, Cao R, et al. A single nucleotide mutation in NS2A of Japanese encephalitis-live vaccine virus (SA14-14-2) ablates NS1' formation and contributes to attenuation. J Gen Virol. 2012;93:1959-64 pubmed publisher
    ..These findings provide critical information in understanding the molecular mechanism of JE vaccine attenuation and is critical for JE vaccine quality control. ..
  9. Lee H, Min K, Lee J, Kang S, Jeon W, Nam J, et al. The prM-independent packaging of pseudotyped Japanese encephalitis virus. Virol J. 2009;6:115 pubmed publisher
    ..These results showed that JEV-MuLV pseudotyped viruses did not require prM for production of infectious pseudotyped viruses. ..

More Information


  1. Hua R, Liu L, Huo H, Li Y, Guo L, Wang X, et al. Comprehensive mapping of a novel NS1 epitope conserved in flaviviruses within the Japanese encephalitis virus serocomplex. Virus Res. 2014;185:103-9 pubmed publisher
    ..The epitope was recognized by antisera against the WNV but not against the dengue virus. This novel JEV serocomplex-specific linear B-cell epitope of NS1 would be helpful in the development of new vaccines and diagnostic assays...
  2. Kao Y, Chang B, Liang J, Tsai H, Lee Y, Lin R, et al. Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid β-oxidation. PLoS Pathog. 2015;11:e1004750 pubmed publisher
    ..We identified a novel function of JEV NS5 in viral pathogenesis by impairing LCFA β-oxidation and inducing cytokine expression by association with MTP. ..
  3. Chen C, Kuo M, Chien L, Hsu S, Wang Y, Lin J. RNA-protein interactions: involvement of NS3, NS5, and 3' noncoding regions of Japanese encephalitis virus genomic RNA. J Virol. 1997;71:3466-73 pubmed
    ..These findings suggest that the 3' NCR of JEV genomic RNA may form a replication complex together with NS3 and NS5; this complex may be involved in JEV minus-strand RNA synthesis...
  4. Upadhyay A, Cyr M, Longenecker K, Tripathi R, Sun C, Kempf D. Crystal structure of full-length Zika virus NS5 protein reveals a conformation similar to Japanese encephalitis virus NS5. Acta Crystallogr F Struct Biol Commun. 2017;73:116-122 pubmed publisher
    ..The structure is similar to that reported for the NS5 protein from Japanese encephalitis virus and suggests opportunities for structure-based drug design targeting either its MTase or RdRp domain. ..
  5. Yuan L, Wu R, Liu H, Wen X, Huang X, Wen Y, et al. The NS3 and NS4A genes as the targets of RNA interference inhibit replication of Japanese encephalitis virus in vitro and in vivo. Gene. 2016;594:183-189 pubmed publisher
    ..This study indicates that RNAi targeting of the NS3 and NS4A genes of JEV can sufficiently inhibit the replication of JEV in vitro and in vivo, and NS3 and NS4A genes might be potential targets of molecular therapy for JEV infection. ..
  6. Brault J, Kudelko M, Vidalain P, Tangy F, Despr s P, Pardigon N. The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication. Virology. 2011;417:369-78 pubmed publisher
    ..Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein...
  7. Poonsiri T, Wright G, Diamond M, Turtle L, Solomon T, Antonyuk S. Structural Study of the C-Terminal Domain of Nonstructural Protein 1 from Japanese Encephalitis Virus. J Virol. 2018;92: pubmed publisher
    ..These structural features are central to NS1 functionality and may provide insight into the development of diagnostic tests and therapeutics. ..
  8. Li M, Wang P, Zheng Z, Hu K, Zhang M, Guan X, et al. Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E. Virology. 2017;510:67-75 pubmed publisher
    ..Our results together indicate that the release of enveloped viruses whose budding take place in an intracellular compartment can be restricted by BST2. ..
  9. Sun E, Zhao J, Liu N, Yang T, Ma J, Geng H, et al. Comprehensive mapping of West Nile virus (WNV)- and Japanese encephalitis virus serocomplex-specific linear B-cell epitopes from WNV non-structural protein 1. J Gen Virol. 2012;93:50-60 pubmed publisher
    ..The knowledge and reagents generated in this study have potential applications in differential diagnostics and epitope-based marker vaccine development for WNV and viruses of the JEV serocomplex...
  10. de Wispelaere M, Khou C, Frenkiel M, Desprès P, Pardigon N. A Single Amino Acid Substitution in the M Protein Attenuates Japanese Encephalitis Virus in Mammalian Hosts. J Virol. 2015;90:2676-89 pubmed publisher
    ..Moreover, infection of mice indicated that the mutant was completely attenuated and triggered a strong immune response to JEV, thus providing new insights for further development of JEV vaccines. ..
  11. Wang B, Hua R, Tian Z, Chen N, Zhao F, Liu T, et al. Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Virus Res. 2009;141:90-5 pubmed publisher
    ..Notably, none of the homologous regions on NS1 proteins of other flavivirus could react with the mAb when they were tested for cross-reactivity, suggesting the potential clinical application of this epitope in differential diagnosis...
  12. Takamatsu Y, Okamoto K, Dinh D, Yu F, Hayasaka D, Uchida L, et al. NS1' protein expression facilitates production of Japanese encephalitis virus in avian cells and embryonated chicken eggs. J Gen Virol. 2014;95:373-83 pubmed publisher
    ..These findings clearly indicate that NS1' enhances the production of JEV in avian cells and may facilitate the amplification/maintenance role of birds in the virus transmission cycle in nature...
  13. Katoh H, Mori Y, Kambara H, Abe T, Fukuhara T, Morita E, et al. Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA. J Virol. 2011;85:10976-88 pubmed publisher
    ..These results suggest that hnRNP A2 plays an important role in the replication of JEV RNA through the interaction with viral proteins and RNA...
  14. Lin C, Cheng C, Yang T, Li S, Cheng M, Wan L, et al. Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42. Virus Res. 2008;137:49-55 pubmed publisher
    ..Therefore, these results show that JEV NS4A without the C-terminal 2K domain is associated with modulation of the IFN response and the interaction of JEV NS4A with RNA helicase DDX42 could be important for JE pathogenesis...
  15. Deng X, Shi Z, Li S, Wang X, Qiu Y, Shao D, et al. Characterization of nonstructural protein 3 of a neurovirulent Japanese encephalitis virus strain isolated from a pig. Virol J. 2011;8:209 pubmed publisher
    ..The Purkinje cell of the cerebellum is one of the target cells of JEV infection. Our data should provide some basic information for the study of the role of NS3 in the pathogenesis of JEV and the immune response...
  16. Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, et al. Involvement of cyclophilin B in the replication of Japanese encephalitis virus. Virology. 2011;412:211-9 pubmed publisher
    ..CypB was colocalized and immunoprecipitated with JEV NS4A in infected cells. These results suggest that CypB plays a crucial role in the replication of JEV through an interaction with NS4A...
  17. Tsuda Y, Mori Y, Abe T, Yamashita T, Okamoto T, Ichimura T, et al. Nucleolar protein B23 interacts with Japanese encephalitis virus core protein and participates in viral replication. Microbiol Immunol. 2006;50:225-34 pubmed
    ..Furthermore, overexpression of dominant negatives of B23 reduced JEV replication. These results suggest that B23 plays an important role in the intracellular localization of the core protein and replication of JEV...
  18. Li X, Ye H, Deng C, Liu S, Zhang H, Shang B, et al. Genetic interaction between NS4A and NS4B for replication of Japanese encephalitis virus. J Gen Virol. 2015;96:1264-75 pubmed publisher
    ..Our results, for the first time to our knowledge, clearly showed the genetic interaction between NS4A and NS4B, although the mechanism underlying their interaction is unknown. ..
  19. Zhu Z, Tian J, Chen B, Peng J, Wu T, Hu Q. [Analysis of sequence and genotype of E gene of the newly isolated Japanese encephalitis virus strains in Wuhan, Hubei Province]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011;25:258-61 pubmed
    ..The vaccine could still be adopted to prevent Japanese encephalitis if steps were take to eradicate mosquitos at the same time. laboratory surveillance were also an important task to build an early-warning mechanism against JEV...
  20. Wu J, Lu G, Zhang B, Gong P. Perturbation in the conserved methyltransferase-polymerase interface of flavivirus NS5 differentially affects polymerase initiation and elongation. J Virol. 2015;89:249-61 pubmed publisher
    ..Taken together, we provide biochemical evidence and mechanistic insights for the cross talk between the two enzyme modules of flavivirus NS5. ..
  21. Surana P, Satchidanandam V, Nair D. RNA-dependent RNA polymerase of Japanese encephalitis virus binds the initiator nucleotide GTP to form a mechanistically important pre-initiation state. Nucleic Acids Res. 2014;42:2758-73 pubmed publisher
    ..Collectively, these observations suggest that the observed pre-initiation state may serve as a checkpoint to prevent erroneous template-independent RNA synthesis by jRdRp during initiation...
  22. Yun S, Song B, Kim J, Yun G, Lee E, Li L, et al. A molecularly cloned, live-attenuated japanese encephalitis vaccine SA14-14-2 virus: a conserved single amino acid in the ij Hairpin of the Viral E glycoprotein determines neurovirulence in mice. PLoS Pathog. 2014;10:e1004290 pubmed publisher
    ..Our results provide a crucial step toward developing novel therapeutic and preventive strategies against JEV and possibly other encephalitic flaviviruses. ..
  23. Moureau G, Cook S, Lemey P, Nougairede A, Forrester N, Khasnatinov M, et al. New insights into flavivirus evolution, taxonomy and biogeographic history, extended by analysis of canonical and alternative coding sequences. PLoS ONE. 2015;10:e0117849 pubmed publisher
    ..We also discuss the significance of programmed ribosomal frameshifting in a central region of the polyprotein open reading frame in some mosquito-associated flaviviruses. ..
  24. Iwai A, Hasumura Y, Nojima T, Takegami T. Hepatitis C virus nonstructural protein NS3 binds to Sm-D1, a small nuclear ribonucleoprotein associated with autoimmune disease. Microbiol Immunol. 2003;47:601-11 pubmed
    ..The localization of NS3 could change following interaction with Sm-D1 and affect the function of Sm-D1...
  25. Ye J, Chen Z, Li Y, Zhao Z, He W, Zohaib A, et al. Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB. J Virol. 2017;91: pubmed publisher
    ..These findings reveal a novel strategy for JEV to escape the host innate immune response and provide new insights into the pathogenesis of JEV. ..
  26. Tseng H, Lee C, Wong M, Chen S, Liu J. DNA-binding property of recombinant capsid protein of Japanese encephalitis virus. Virus Genes. 2007;35:483-8 pubmed publisher
    ..This study suggests a possible regulatory role for capsid protein in the pathway of JEV infection...
  27. Deng W, Guan C, Liu K, Zhang X, Feng X, Zhou B, et al. Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody. Virus Res. 2014;179:133-9 pubmed publisher
  28. Yamaguchi Y, Nukui Y, Tajima S, Nerome R, Kato F, Watanabe H, et al. An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro. J Gen Virol. 2011;92:1601-6 pubmed publisher
    ..This is the first report to show that an amino acid substitution in a flavivirus NS4A protein alters viral pathogenicity in mice. ..
  29. Li X, Deng C, Ye H, Zhang H, Zhang Q, Chen D, et al. Transmembrane Domains of NS2B Contribute to both Viral RNA Replication and Particle Formation in Japanese Encephalitis Virus. J Virol. 2016;90:5735-5749 pubmed publisher
    ..The results have uncovered a new function of flavivirus NS2B in virion assembly, possibly through interaction with the NS2A protein. ..
  30. Chiou S, Fan Y, Crill W, Chang R, Chang G. Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein. J Gen Virol. 2012;93:1185-92 pubmed publisher
    ..The amino acid residues of both domains II and III of the E protein were shown to be important in the binding of JEV serocomplex cross-reactive mAbs...
  31. Pujhari S, Prabhakar S, Ratho R, Modi M, Sharma M, Mishra B. A novel mutation (S227T) in domain II of the envelope gene of Japanese encephalitis virus circulating in North India. Epidemiol Infect. 2011;139:849-56 pubmed publisher
    ..Genotype III was found to be circulating in this geographical area. Further studies are required to ascertain its role in JE pathogenesis and vector competency. ..
  32. Zhang Z, Wang X, Zai J, Sun L, Song Y, Chen H. [Roles of N-glycosylation in immunity of prME and NS1 gene of JEV]. Bing Du Xue Bao. 2012;28:213-8 pubmed
    ..Thus these results show that the N-linked glycosylation in the prME and NS1 gene were correlated with the immunity, one glycan absent would enhance the immunity but both two loss would impair it. ..
  33. Wang P, Hu K, Luo S, Zhang M, Deng X, Li C, et al. DC-SIGN as an attachment factor mediates Japanese encephalitis virus infection of human dendritic cells via interaction with a single high-mannose residue of viral E glycoprotein. Virology. 2016;488:108-19 pubmed publisher
    ..Our findings highlight the potential significance of DC-SIGN in JEV early infection, providing a basis for further understanding how JEV exploits DC-SIGN to gain access to dendritic cells. ..
  34. Tu Y, Yu C, Liang J, Lin E, Liao C, Lin Y. Blocking double-stranded RNA-activated protein kinase PKR by Japanese encephalitis virus nonstructural protein 2A. J Virol. 2012;86:10347-58 pubmed publisher
    ..The recombinant JEV mutant carrying the NS2A-T33I mutation showed reduced in vitro growth and in vivo virulence phenotypes. Thus, JEV NS2A has a novel function in blocking the host antiviral response of PKR during JEV infection...