ZP2

Summary

Gene Symbol: ZP2
Description: zona pellucida glycoprotein 2 (sperm receptor)
Alias: ZPA, Zp-2, zona pellucida glycoprotein ZP2, zona pellucida protein A, zona pellucida sperm-binding protein 2
Species: human

Top Publications

  1. ncbi Immunogenicity and contraceptive potential of three infertility-relevant zona pellucida 2 epitopes in the marsupial brushtail possum (Trichosurus vulpecula)
    Janine A Duckworth
    National Research Centre for Possum Biocontrol at Landcare Research, PO Box 40, Lincoln 7640, New Zealand
    Reproduction 133:177-86. 2007
  2. ncbi Structural conservation of mouse and rat zona pellucida glycoproteins. Probing the native rat zona pellucida proteome by mass spectrometry
    Emily S Boja
    Laboratory of Applied Mass Spectrometry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 44:16445-60. 2005
  3. ncbi Feasibility and challenges in the development of immunocontraceptive vaccine based on zona pellucida glycoproteins
    Sangeeta Choudhury
    Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Soc Reprod Fertil Suppl 63:479-93. 2007
  4. ncbi Hamster zona pellucida is formed by four glycoproteins: ZP1, ZP2, ZP3, and ZP4
    M J Izquierdo-Rico
    Department of Cell Biology, School of Medicine, University of Murcia, 30100 Murcia, Spain
    J Proteome Res 8:926-41. 2009
  5. ncbi Structural analysis of the asparagine-linked glycan units of the ZP2 and ZP3 glycoproteins from mouse zona pellucida
    D R Tulsiani
    Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2633, USA
    Arch Biochem Biophys 382:275-83. 2000
  6. ncbi A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction
    Hiroki Okumura
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    Biochem J 384:191-9. 2004
  7. ncbi Expression in Escherichia coli and immunological characterization of three zona pellucida proteins (ZP1, ZP2, and ZP3) from a marsupial, the brushtail possum (Trichosurus vulpecula)
    Karen E Mate
    Cooperative Research Centre for Conservation and Management of Marsupials, Department of Biological Sciences, Macquarie University, Australia
    Mol Reprod Dev 64:136-43. 2003
  8. ncbi Distinct membrane fractions from mouse sperm bind different zona pellucida glycoproteins
    Catherine D Thaler
    Department of Biology, University of Central Florida, Orlando, Florida 32816, USA
    Biol Reprod 66:65-9. 2002
  9. ncbi Effects of 17a-ethinylestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish
    Markus Islinger
    Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, D 69120, Heidelberg, Germany
    Aquat Toxicol 62:85-103. 2003
  10. ncbi Regulation of mouse egg activation: presence of ryanodine receptors and effects of microinjected ryanodine and cyclic ADP ribose on uninseminated and inseminated eggs
    T Ayabe
    Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104, USA
    Development 121:2233-44. 1995

Research Grants

  1. OOCYTE CORTICAL MATURATION AND GRANULE ACTIVATION
    THOMAS DUCIBELLA; Fiscal Year: 1993
  2. Gap Junctions/Limb Outgrowth, Patterning, Skeletogenesis
    CAROLINE DEALY; Fiscal Year: 2004
  3. A novel regulator of PI-3-Kinase in human liver cancer
    Marie DeFrances; Fiscal Year: 2007
  4. VERTEBRATE ORGANIZER FORMATION CELL AND MOLECULAR CUES
    Lee Niswander; Fiscal Year: 2000
  5. Regulation of Shh Signaling Activity in Limb Patterning
    Chin Chiang; Fiscal Year: 2007
  6. NOTCH SIGNALING AND VERTEBRATE LIMB DEVELOPMENT
    Juan Carlos Izpisua Belmonte; Fiscal Year: 2003
  7. Regulation of Shh Signaling Activity in Limb Patterning
    Chin Chiang; Fiscal Year: 2010
  8. EPITHELIAL/MESENCHYMAL INTERACTIONS IN LIMB PATTERNING
    John Fallon; Fiscal Year: 2009
  9. SONIC HEDGEHOG'S ROLE IN PATTERNING THE LIMB
    Clifford Tabin; Fiscal Year: 1999
  10. A Lineage Analysis of Cells in the Vertebrate Limb
    JENNIFER MANSFIELD; Fiscal Year: 2005

Scientific Experts

Detail Information

Publications143 found, 100 shown here

  1. ncbi Immunogenicity and contraceptive potential of three infertility-relevant zona pellucida 2 epitopes in the marsupial brushtail possum (Trichosurus vulpecula)
    Janine A Duckworth
    National Research Centre for Possum Biocontrol at Landcare Research, PO Box 40, Lincoln 7640, New Zealand
    Reproduction 133:177-86. 2007
    In a previous study, three infertility-relevant epitopes of possum ZP2 (Pep12 (amino acids 111-125), Pep31 (amino acids 301-315), and Pep44 (amino acids 431-445)) were identified using sera from possums (Trichosurus vulpecula) immunized ..
  2. ncbi Structural conservation of mouse and rat zona pellucida glycoproteins. Probing the native rat zona pellucida proteome by mass spectrometry
    Emily S Boja
    Laboratory of Applied Mass Spectrometry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 44:16445-60. 2005
    ..Mouse zonae are composed of three glycoproteins (ZP1, ZP2, and ZP3), while rat zonae contain four (ZP1, ZP2, ZP3, and ZP4/ZPB)...
  3. ncbi Feasibility and challenges in the development of immunocontraceptive vaccine based on zona pellucida glycoproteins
    Sangeeta Choudhury
    Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Soc Reprod Fertil Suppl 63:479-93. 2007
    ..In order to evaluate the immunocontraceptive potential of ZP glycoproteins, bonnet monkey (Macaca radiata) ZP2, ZP3 and ZP4 have been cloned and expressed using either E. coli or baculovirus expression systems...
  4. ncbi Hamster zona pellucida is formed by four glycoproteins: ZP1, ZP2, ZP3, and ZP4
    M J Izquierdo-Rico
    Department of Cell Biology, School of Medicine, University of Murcia, 30100 Murcia, Spain
    J Proteome Res 8:926-41. 2009
    ..Recent data have shown the presence of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) in the ZP of human and rat rather than the three glycoproteins proposed in the mouse model...
  5. ncbi Structural analysis of the asparagine-linked glycan units of the ZP2 and ZP3 glycoproteins from mouse zona pellucida
    D R Tulsiani
    Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2633, USA
    Arch Biochem Biophys 382:275-83. 2000
    ..In the mouse, the ZP is composed of three glycoproteins, namely ZP1 (200 kDa), ZP2 (120 kDa), and ZP3 (83 kDa)...
  6. ncbi A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction
    Hiroki Okumura
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    Biochem J 384:191-9. 2004
    ..ZPD belongs phylogenetically to a distinct group from known ZP glycoprotein subfamilies, ZPA, ZPB, and ZPC...
  7. ncbi Expression in Escherichia coli and immunological characterization of three zona pellucida proteins (ZP1, ZP2, and ZP3) from a marsupial, the brushtail possum (Trichosurus vulpecula)
    Karen E Mate
    Cooperative Research Centre for Conservation and Management of Marsupials, Department of Biological Sciences, Macquarie University, Australia
    Mol Reprod Dev 64:136-43. 2003
    ..possum (Trichosurus vulpecula) zona pellucida (ZP) is composed of three major glycoproteins, designated ZP1, ZP2, and ZP3 based on their size and homology with eutherian ZP proteins...
  8. ncbi Distinct membrane fractions from mouse sperm bind different zona pellucida glycoproteins
    Catherine D Thaler
    Department of Biology, University of Central Florida, Orlando, Florida 32816, USA
    Biol Reprod 66:65-9. 2002
    ..ZP fractions consisting of a ZP3-enriched fraction (125I-ZP3) and a fraction enriched for ligands ZP1 and ZP2 and depleted of ZP3 (125I-ZP1/2) were obtained by antibody affinity purification of ZP3...
  9. ncbi Effects of 17a-ethinylestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish
    Markus Islinger
    Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, D 69120, Heidelberg, Germany
    Aquat Toxicol 62:85-103. 2003
    ..on the reproductive physiology of fish, molecular markers for zebrafish vitellogenin, estrogen receptor and ZP2 were developed...
  10. ncbi Regulation of mouse egg activation: presence of ryanodine receptors and effects of microinjected ryanodine and cyclic ADP ribose on uninseminated and inseminated eggs
    T Ayabe
    Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104, USA
    Development 121:2233-44. 1995
    ..Ryanodine induced the conversion of the zona pellucida glycoprotein ZP2 to its postfertilization form ZP2f in a biphasic concentration-dependent manner; nanomolar ..
  11. ncbi Mouse zona pellucida genes and glycoproteins
    P M Wassarman
    Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Cytogenet Genome Res 105:228-34. 2004
    ..The mouse ZP consists of only three glycoproteins, called ZP1, ZP2, and ZP3. All three glycoproteins are essential structural components of the ZP...
  12. ncbi Molecular cloning of bovine zona pellucida glycoproteins ZPA and ZPB and analysis for sperm-binding component of the zona
    N Yonezawa
    Graduate School of Science and Technology, and Department of Chemistry, Chiba University, Japan
    Eur J Biochem 268:3587-94. 2001
    The zona pellucida, a transparent envelope surrounding the mammalian oocyte, comprises three glycoproteins, ZPA, ZPB and ZPC, and plays important roles in fertilization...
  13. ncbi Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica)
    Mihoko Kinoshita
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422 8529, Japan
    Reproduction 139:359-71. 2010
    ..In this study, we report the isolation of cDNA encoding quail ZP2 and its expression in the female bird...
  14. ncbi Quantification of rainbow trout (Oncorhynchus mykiss) zona radiata and vitellogenin mRNA levels using real-time PCR after in vivo treatment with estradiol-17 beta or alpha-zearalenol
    T Celius
    National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824 1319, USA
    J Steroid Biochem Mol Biol 75:109-19. 2000
    ..ZR-gene showed a high degree of amino acid sequence identity to that of salmon (77%), winter flounder (64%), carp ZP2 (63%) and medaka (61%) ZR-proteins...
  15. ncbi Inhibition of zona pellucida gene expression by antisense oligonucleotides injected into mouse oocytes
    Z B Tong
    Developmental Endocrinology Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:849-53. 1995
    During murine oogenesis, the zona pellucida proteins (ZP1, ZP2, and ZP3) are synthesized and secreted to form an extracellular matrix that surrounds the oocyte and mediates specific biological functions essential to mammalian ..
  16. ncbi Biological approaches to bone regeneration by gene therapy
    R T Franceschi
    University of Michigan School of Dentistry, 1011 N University Ave, Ann Arbor, MI 48109 1078, USA
    J Dent Res 84:1093-103. 2005
    ..Abbreviations used: BMP, bone morphogenetic protein; FGF, fibroblast growth factor; AER, apical ectodermal ridge; ZPA, zone of polarizing activity; PZ, progress zone; SHH, sonic hedgehog; OSX, osterix transcription factor; FGFR, ..
  17. ncbi Immunohistochemical localization of zona pellucida proteins ZPA, ZPB and ZPC in human, cynomolgus monkey and mouse ovaries
    U Eberspaecher
    Preclinical Drug Research, Schering AG, Berlin, Germany
    Cell Tissue Res 303:277-87. 2001
    ..The genes coding for the three zona pellucida glycoproteins ZPA, ZPB, and ZPC of various species including mouse, dog, and human have been cloned and sequenced by several groups...
  18. ncbi Marsupial egg and embryo coats
    L Selwood
    Department of Zoology, La Trobe University, Bundoora, Vic, Australia
    Cells Tissues Organs 166:208-19. 2000
    ..The zona pellucida consists of three zona proteins (ZPA, ZPB, ZPC) with considerable similarity to those of eutherian mammals...
  19. ncbi Rat zona pellucida glycoproteins: molecular cloning and characterization of the three major components
    K Akatsuka
    Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata City, Japan
    Mol Reprod Dev 51:454-67. 1998
    ..Significant homologies were observed between rZP2 and ZPA family from various mammalian species...
  20. ncbi Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)
    Petra Walcher
    Department of Medicinal Chemistry, University of Vienna, Althanstrasse 14, A 1090 Vienna, Austria
    Vaccine 26:6832-8. 2008
    ..Immunisation of female possums against recombinant possum zona pellucida protein-2 (ZP2) is known to reduce embryo production by 72-75% but successful development of fertility control will depend on a ..
  21. ncbi [Molecules involved in sperm-zona pellucida interaction in mammals. Role in human fertility]
    Catherine Serres
    Universite Paris Descartes, Faculté de Cochin, Laboratoire de Biologie de la Reproduction, 24 rue du Faubourg Saint Jacques, 75014 Paris, France
    J Soc Biol 202:119-28. 2008
    ..which induces the sperm acrosome exocytosis followed by a secondary binding of acrosome reacted spermatozoa to ZP2 and by ZP penetration...
  22. ncbi Human zona pellucida glycoproteins: functional relevance during fertilization
    Satish K Gupta
    Reproductive Cell Biology Laboratory, National Institute of Immunology, New Delhi, India
    J Reprod Immunol 83:50-5. 2009
    ..In contrast to mouse, human ZP matrix is composed of 4 glycoproteins designated as ZP1, ZP2, ZP3 and ZP4 (Zp4 pseudogene in mouse)...
  23. ncbi Biosynthesis of the canine zona pellucida requires the integrated participation of both oocytes and granulosa cells
    Daniel G Blackmore
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 71:661-8. 2004
    ..Riboprobes for canine ZPA and ZPC genes were produced and used for in situ hybridization studies of mRNA expression in canine ..
  24. ncbi Coordinate expression of the three zona pellucida genes during mouse oogenesis
    O Epifano
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Development 121:1947-56. 1995
    ..The mouse zona is composed of three sulfated glycoproteins: ZP1, ZP2 and ZP3...
  25. ncbi Regulation of zebrafish zona pellucida gene activity in developing oocytes
    David E Mold
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biol Reprod 81:101-10. 2009
    The two major zona pellucida proteins of the zebrafish chorion, Zp2 and Zp3, are encoded by multicopy genes arranged in tandem arrays on chromosomes 20 and 2, respectively...
  26. ncbi Recombinant porcine zona pellucida glycoproteins expressed in Sf9 cells bind to bovine sperm but not to porcine sperm
    Naoto Yonezawa
    Graduate School of Science and Technology, Department of Chemistry, Faculty of Science, Chiba University Japan
    J Biol Chem 280:20189-96. 2005
    The zona pellucida, which surrounds the mammalian oocyte, consists of the ZPA, ZPB, and ZPC glycoproteins and plays roles in species-selective sperm-egg interactions via its carbohydrate moieties...
  27. ncbi Structural characterization of native mouse zona pellucida proteins using mass spectrometry
    Emily S Boja
    Laboratory of Biophysical Chemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:34189-202. 2003
    ..The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA...
  28. ncbi Structural significance of N-glycans of the zona pellucida on species-selective recognition of spermatozoa between pig and cattle
    N Yonezawa
    Graduate School of Science and Technology, Chiba University, 1 33, Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Soc Reprod Fertil Suppl 63:217-28. 2007
    ..In the pig and cattle, the zona pellucida consists of ZPA, ZPB and ZPC...
  29. ncbi Amino acid sequence of a porcine zona pellucida glycoprotein ZP4 determined by peptide mapping and cDNA cloning
    A Hasegawa
    Department of Obstetrics and Gynecology, Hyogo Medical College, Nishinomiya, Japan
    J Reprod Fertil 100:245-55. 1994
    ..The 128 amino acid peptide showed 39.1% similarity to the amino-terminal region of mouse ZP2 polypeptide. The positions of five cysteine residues were the same for porcine ZP4 and mouse ZP2...
  30. ncbi Molecular characterization and expression pattern of two zona pellucida genes in half-smooth tongue sole (Cynoglossus semilaevis)
    Yeying Sun
    College of Pharmacy, Binzhou Medical University, Yantai 264003, China
    Comp Biochem Physiol B Biochem Mol Biol 155:316-21. 2010
    ..ZP) proteins are glycoproteins in fish chorion that are encoded by multiple gene families, mainly including zp1, zp2, and zp3...
  31. ncbi Sequential expression of zona pellucida protein genes during the oogenesis of domestic cats
    K Jewgenow
    Institute for Zoo Biology and Wildlife Research, D 10252 Berlin, Germany
    Biol Reprod 60:522-6. 1999
    ..The generated cDNAs were used for polymerase chain reaction (PCR) with specific feline ZPA, ZPB, and ZPC gene primers. All amplified products were sequenced to confirm their identity...
  32. ncbi Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells
    Ming Zhao
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 43:12090-104. 2004
    ..The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, ZP3)...
  33. ncbi Prospect for immunocontraception using the NH2-terminal recombinant peptide of human zona pellucida protein (hZPA)
    Koji Koyama
    Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan
    Am J Reprod Immunol 47:303-10. 2002
    ..Our previous study showed that a human zona pellucida (ZPA) recombinant protein produced antiserum that inhibited human in vitro fertilization in rabbits...
  34. ncbi Mutations in mouse Aristaless-like4 cause Strong's luxoid polydactyly
    S Qu
    Departments of Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Development 125:2711-21. 1998
    ..of the limb axes depends on several interacting signaling centers; one of these, the zone of polarizing activity (ZPA), comprises a group of mesenchymal cells along the posterior aspect of the limb bud that express sonic hedgehog (..
  35. ncbi ZP2 and ZP3 traffic independently within oocytes prior to assembly into the extracellular zona pellucida
    Tanya Hoodbhoy
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Mol Cell Biol 26:7991-8. 2006
    ..In mice, the zona pellucida is composed of three glycoproteins, but the presence of ZP2 and ZP3 is sufficient to form a biologically functional structure...
  36. ncbi Biosynthesis and expression of zona pellucida glycoproteins in mammals
    F Sinowatz
    Lehrstuhl für Tieranatomie II, University of Munich, Germany
    Cells Tissues Organs 168:24-35. 2001
    ..The ZP of most mammalian species is composed of three glycoproteins (ZPA, ZPB, ZPC), products of the gene families ZPA, ZPB and ZPC that have been found to be highly homologous within ..
  37. ncbi Purified mouse egg zona pellucida glycoproteins polymerize into homomeric fibrils under non-denaturing conditions
    Eveline S Litscher
    Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Cell Physiol 214:153-7. 2008
    The mouse egg's zona pellucida (ZP) is composed of three glycoproteins, called ZP1, ZP2, and ZP3, that migrate as relatively broad, single bands on SDS-PAGE...
  38. ncbi Contribution of zona proteins to oocyte growth
    Akiko Hasegawa
    Laboratory of Developmental Biology and Reproduction, Institute for Advanced Medical Sciences, Hyogo College of Medicine, 1 1, Mukogawa cho, Nishinomiya 663 8501, Japan
    Soc Reprod Fertil Suppl 63:229-35. 2007
    ..In mice, the zona pellucida is composed of three glycoproteins designated as ZPA, ZPB and ZPC. ZpA and ZpC knockout mice are infertile due to incomplete folliculogenesis...
  39. ncbi Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development
    T L Rankin
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Development 128:1119-26. 2001
    ..The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2 and ZP3), each encoded by a single copy gene...
  40. ncbi Functional morphology of the zona pellucida
    F Sinowatz
    Lehrstuhl für Tieranatomie II, University of Munich, Germany
    Anat Histol Embryol 30:257-63. 2001
    ..genes of a variety of mammalian species including domestic animals show a high homology, three classes of ZP genes, ZPA, ZPB and ZPC can be discerned. The corresponding proteins were named ZPA, ZPB and ZPC...
  41. ncbi Developmental basis for hind-limb loss in dolphins and origin of the cetacean bodyplan
    J G M Thewissen
    Department of Anatomy, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA
    Proc Natl Acad Sci U S A 103:8414-8. 2006
    ..is maintained by two signaling centers, the apical ectodermal ridge (AER) and the zone of polarizing activity (ZPA)...
  42. ncbi Incorporation of mouse zona pellucida proteins into the envelope of Xenopus laevis oocytes
    S Doren
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, 6 Center DR MSC 2715, Bethesda, MD 20892 2715, USA
    Dev Genes Evol 209:330-9. 1999
    ..The mouse zona, composed of three sulfated glycoproteins (ZP1, ZP2, ZP3), is critical for fertilization and early development, and mice lacking a zona pellucida produce no live ..
  43. ncbi The zona pellucida 'receptors' ZP1, ZP2 and ZP3
    K D Hinsch
    Centre of Dermatology and Andrology, Justus Liebig University Giessen, Germany
    Andrologia 31:320-2. 1999
    ..In the mouse, ZP1 is the homodimeric filament crosslinker, held together by intermolecular disulphides. ZP2 is the 'secondary receptor', which is cleaved by egg proteases after egg activation...
  44. ncbi Oocyte-specific expression of mouse Zp-2: developmental regulation of the zona pellucida genes
    L F Liang
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    Mol Cell Biol 10:1507-15. 1990
    ..The genes that code for two of the mouse zona proteins (ZP2 and ZP3) represent a developmentally regulated set of genes whose expression serves as markers of mouse oocyte ..
  45. ncbi Mutation of a conserved hydrophobic patch prevents incorporation of ZP3 into the zona pellucida surrounding mouse eggs
    Ming Zhao
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health, Building 50, Bethesda, MD 20892, USA
    Mol Cell Biol 23:8982-91. 2003
    Three glycoproteins (ZP1, ZP2, and ZP3) are synthesized in growing mouse oocytes and secreted to form an extracellular zona pellucida that mediates sperm binding and fertilization...
  46. ncbi Formation of polyelectrolyte multilayers by flexible and semiflexible chains
    Bo Wu
    Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei, PR China 230026
    J Phys Chem B 116:3106-14. 2012
    ..By use of a quartz crystal microbalance with dissipation (QCM-D), a zeta potential analyzer (ZPA), and atomic force microscopy (AFM), we have investigated the growth of PSS/PDDA, PSS/CGG, SC/PDDA, and SC/CGG ..
  47. ncbi Opposing functions of the ETS factor family define Shh spatial expression in limb buds and underlie polydactyly
    Laura A Lettice
    MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Dev Cell 22:459-67. 2012
    ..The spatial pattern of Shh expression is restricted to a region called the zone of polarizing activity (ZPA), and this expression is controlled from a long distance by the cis-regulator ZRS...
  48. ncbi Identification and characterization of a unique Xenopus laevis egg envelope component, ZPD
    Leann L Lindsay
    Section of Molecular and Cellular Biology, University of California, Davis 95616, USA
    Dev Growth Differ 44:205-12. 2002
    ..that it is most closely related (34% amino acid identity) to the ZP domains of mammalian tectorin, uromodulin and ZPA. From a dendrogram of known egg envelope glycoproteins, the new glycoprotein was shown to be unique among egg ..
  49. ncbi Assembly of the inner perivitelline layer, a homolog of the mammalian zona pellucida: an immunohistochemical and ultrastructural study
    Daniela Rodler
    Institute of Anatomy, Histology and Embryology, Department of Veterinary Sciences, University of Munich, Munich, Germany
    Cells Tissues Organs 195:330-9. 2012
    ..shown that in the Japanese quail (Coturnix japonica) at least 5 glycoproteins are constituents of the IPVL (ZP1, ZP2, ZP3, ZP4, and ZPD)...
  50. ncbi A duplicated zone of polarizing activity in polydactylous mouse mutants
    H Masuya
    Mammalian Genetics Laboratory, National Institute of Genetics, Mishima, Japan
    Genes Dev 9:1645-53. 1995
    ..along the anteroposterior axis of the developing vertebrate limb is provided by the zone of polarizing activity (ZPA) located at the posterior margin. Recently, it was established that the Sonic hedgehog (Shh) mediates ZPA activity...
  51. ncbi Cloning of a cDNA coding for porcine zona pellucida glycoprotein ZP1 and its genomic organization
    T Taya
    Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan
    Biochem Biophys Res Commun 207:790-9. 1995
    ..ZP1, one of the major glycoproteins of porcine zona pellucida, was divided into two components (porcine ZP4 and ZP2), and suggested it was a homologue of mouse ZP2...
  52. ncbi 195 ALTERED mRNA TRANSCRIPT EXPRESSION OF IN VITRO- VERSUS IN VIVO-MATURED PORCINE OOCYTES
    L D Spate
    University of Missouri, Columbia, MO, USA
    Reprod Fertil Dev 24:210. 2011
    ..Eight transcripts were selected for real-time PCR. ZP2 was higher in the in vitro-matured oocytes as determined by both sequencing and real time...
  53. ncbi Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex
    Saeko Kanai
    Graduate School of Science and Technology, Chiba University, Japan
    FEBS J 274:5390-405. 2007
    ..Porcine and bovine ZPs are composed of the glycoproteins ZP2, ZP3, and ZP4...
  54. ncbi Developmental analysis of the retinoic acid-inducible RAR-beta 2 promoter in transgenic animals
    C Mendelsohn
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculte de Medecine, Strasbourg, France
    Development 113:723-34. 1991
    ..or mRAR-beta transcripts were associated with the limb region that contains the zone of polarizing activity (ZPA)...
  55. ncbi Cross-fertilization and structural comparison of egg extracellular matrix glycoproteins from Xenopus laevis and Xenopus tropicalis
    L L Lindsay
    Section of Molecular and Cellular Biology, University of California, One Shields Avenue, Davis, CA 95616, USA
    Comp Biochem Physiol A Mol Integr Physiol 136:343-52. 2003
    ..The cDNA sequences for egg envelope glycoproteins ZPA, ZPB, ZPC, ZPD and ZPAX, and also egg cortical granule lectin involved in the block to polyspermy, were cloned for ..
  56. ncbi Structural, biochemical and functional aspects of sperm-oocyte interactions in pigs
    D Rath
    Institute for Animal Breeding, Mariensee, FAL 31535 Neustadt, Germany
    Soc Reprod Fertil Suppl 62:317-30. 2006
    ..3 pl units for ZPB/ZPC and 0.8 pl units for ZPA in 2D gel electrophoresis, which is based on increasing sulfation of the oligosaccharides during maturation...
  57. ncbi Relevance of glycosylation of human zona pellucida glycoproteins for their binding to capacitated human spermatozoa and subsequent induction of acrosomal exocytosis
    S Chakravarty
    Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India
    Mol Reprod Dev 75:75-88. 2008
    ..coli)- and baculovirus-expressed recombinant human ZP glycoprotein-2 (ZP2), -3 (ZP3), and -4 (ZP4) were employed...
  58. ncbi Characterization of human sperm binding to homologous recombinant zona pellucida proteins
    Mayel Chirinos
    Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Tlalpan, Mexico DF, Mexico
    Reprod Sci 18:876-85. 2011
    ..The cDNAs coding for human ZP2, ZP3, and ZP4 were expressed in Sf9 cells and proteins were characterized to determine their competence for sperm ..
  59. ncbi Proteolysis of Xenopus laevis egg envelope ZPA triggers envelope hardening
    Leann L Lindsay
    Department of Animal Science, University of California, Davis, CA 95616, United States
    Biochem Biophys Res Commun 324:648-54. 2004
    ..and mammals a prominent feature of envelope modification is N-terminal proteolysis of the envelope glycoprotein ZPA. We have purified the ZPA protease from Xenopus laevis eggs and characterized it as a zinc metalloprotease...
  60. ncbi Identification of the true human orthologue of the mouse Zp1 gene: evidence for greater complexity in the mammalian zona pellucida?
    D C Hughes
    Reproductive Biology and Genetics Group, Assisted Conception Unit, Birmingham Women s Hospital, Birmingham, UK
    Biochim Biophys Acta 1447:303-6. 1999
    ..zona pellucida is a mixture of glycoproteins, believed to be encoded by three distinct genes, ZP1/ZPB, ZP2/ZPA, and ZP3/ZPC...
  61. ncbi SF/HGF is a mediator between limb patterning and muscle development
    M Scaal
    Institute of Anatomy, University of Freiburg, Albertstrasse 17, D 79104 Freiburg, Germany
    Development 126:4885-93. 1999
    ..However, FGF-2 application can rescue SF/HGF expression. Excision of the zone of polarizing activity (ZPA) results in ectopic and enhanced SF/HGF expression in the posterior limb bud mesenchyme...
  62. ncbi Contraceptive potential of porcine and feline zona pellucida A, B and C subunits in domestic cats
    Joyce A Eade
    Faculty of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Western Australia, Australia
    Reproduction 137:913-22. 2009
    ..However, vaccination of cats with feline ZPA or feline ZPB+C DNA vectors elicited circulating antibodies specific for feline ZP as assessed by ELISA, with ..
  63. ncbi Cholesterol modification of sonic hedgehog is required for long-range signaling activity and effective modulation of signaling by Ptc1
    P M Lewis
    Department of Molecular and Cellular Biology, The Biolabs, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 105:599-612. 2001
    Sonic hedgehog (Shh) signaling from the posterior zone of polarizing activity (ZPA) is the primary determinant of anterior-posterior polarity in the vertebrate limb field...
  64. ncbi Exploring polymorphisms and effects of candidate genes on milk fat quality in dairy sheep
    A Crisà
    Consiglio per la Ricerca e la Sperimentazione in Agricoltura, 00015 Monterotondo, Italy
    J Dairy Sci 93:3834-45. 2010
    ..The current study evaluated the global effects of a large number of single nucleotide polymorphisms and haplotypes on traits that are not commonly investigated in sheep but that are potentially very useful for improving milk quality...
  65. ncbi Parthenogenetic embryo-like structures in the human ovarian surface epithelium cell culture in postmenopausal women with no naturally present follicles and oocytes
    Irma Virant-Klun
    Department of Obstetrics and Gynecology, University Medical Centre, Ljubljana, Slovenia
    Stem Cells Dev 18:137-49. 2009
    ..cells developed among them, which reached a diameter up to 95 mum, and expressed Oct-4, c-kit, VASA, and ZP2 transcription markers after 20 days of culture...
  66. ncbi Sequence and analysis of zona pellucida 2 cDNA (ZP2) from a marsupial, the brushtail possum, Trichosurus vulpecula
    K E Mate
    Cooperative Research Centre for Conservation and Management of Marsupials, School of Biological Sciences, Macquarie University, Sydney, NSW, Australia
    Mol Reprod Dev 51:322-9. 1998
    ..This study reports the isolation and characterization of the ZP2 gene from the brushtail possum (Trichosurus vulpecula)...
  67. ncbi Inactivation of the Mgat1 gene in oocytes impairs oogenesis, but embryos lacking complex and hybrid N-glycans develop and implant
    Shaolin Shi
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, New York, NY 10461, USA
    Mol Cell Biol 24:9920-9. 2004
    ..pellucida (ZP) that was thinner than the control ZP, and they did not possess complex N-glycans but contained ZP1, ZP2, and ZP3 glycoproteins. Mutant eggs were fertilized, all embryos implanted, and heterozygotes developed to birth...
  68. ncbi Localization of carbohydrate chains of pig sperm ligand in the glycoprotein ZPB of egg zona pellucida
    K Kudo
    Graduate School of Science and Technology, Chiba University, Japan
    Eur J Biochem 252:492-9. 1998
    The three glycoproteins of pig zona pellucida (ZPA, ZPB and ZPC) can be separated into ZPA and a mixture of ZPB/ZPC by gel-filtration HPLC...
  69. ncbi Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins
    Hongguo Cao
    Anhui Local Livestock Genetic Resources Conservation and Breeding Laboratory, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China
    Int J Biol Sci 8:498-511. 2012
    ..defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3...
  70. ncbi Acrosin antibodies and infertility. I. Detection of antibodies towards proacrosin/acrosin in women consulting for infertility and evaluation of their effects upon the sperm protease activities
    Carolina Veaute
    Instituto de Biologia y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires, Buenos Aires, Argentina
    Fertil Steril 91:1245-55. 2009
    ..To detect the presence of antibodies to the proacrosin/acrosin system and to evaluate their effect on the sperm acrosomal protein activities in women consulting for infertility...
  71. ncbi Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm
    Catherine Patrat
    Biologie de la Reproduction, Hopital Cochin, AP HP, Universite Paris Descartes, Faculte de Medecine, Paris, France
    BMC Dev Biol 6:59. 2006
    The functions of three zona glycoproteins, ZP1, ZP2 and ZP3 during the sperm-zona pellucida (ZP) interaction are now well established in mice...
  72. ncbi Extended exposure to Sonic hedgehog is required for patterning the posterior digits of the vertebrate limb
    Paul J Scherz
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Dev Biol 308:343-54. 2007
    ..We find that maintaining a low level of Shh production throughout the normal time frame of ZPA signaling results in a near complete restoration of the posterior-most digits...
  73. ncbi Structural and functional attributes of zona pellucida glycoproteins
    Satish K Gupta
    Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 1 10 067, India
    Soc Reprod Fertil Suppl 63:203-16. 2007
    ..of the genes of the human oocyte revealed that the ZP matrix is composed of four glycoproteins, designated as ZP1, ZP2, ZP3 and ZP4, instead of 3 found in the mouse ZP...
  74. ncbi Sperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis
    Boris Baibakov
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Development 134:933-43. 2007
    At fertilization, spermatozoa bind to the zona pellucida (ZP1, ZP2, ZP3) surrounding ovulated mouse eggs, undergo acrosome exocytosis and penetrate the zona matrix before gamete fusion...
  75. ncbi How to make a zone of polarizing activity: insights into limb development via the abnormality preaxial polydactyly
    Robert E Hill
    MRC Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh, EH4 2XU, UK
    Dev Growth Differ 49:439-48. 2007
    ..margin is ultimately responsible for the asymmetry due to a region called the zone of polarizing activity (ZPA). The ZPA produces and secretes the molecule SHH, which coordinates the patterning of the resulting digits...
  76. ncbi The molecular genetics of the zona pellucida: mouse mutations and infertility
    T Rankin
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 2715, USA
    Mol Hum Reprod 2:889-94. 1996
    ..The genes that encode the three zona pellucida proteins (ZP1, ZP2, ZP3) have been characterized in mouse and human...
  77. ncbi Anti-human proacrosin antibody inhibits the zona pellucida (ZP)-induced acrosome reaction of ZP-bound spermatozoa
    Carolina Veaute
    Instituto de Biología y Medicina Experimental IBYME, National Research Council of Argentina CONICET, 1428ADN, Buenos Aires, Argentina
    Fertil Steril 93:2456-9. 2010
    ..monoclonal antibody AcrC5F10 inhibited proacrosin activation, proacrosin-human zona pellucida glycoprotein A (ZPA) binding, and the zona pellucida (ZP)-induced acrosome reaction of the ZP-bound spermatozoa but had no significant ..
  78. ncbi Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does
    Yan Xie
    Institute of Reproductive Immunology, Jinan University, Guangzhou 510632, China
    Cell Biochem Funct 28:661-7. 2010
    ..pellucida 2 (mZP2) protein, RT-PCR, and in situ hybridization were carried out to localize the expression of mouse ZP2 mRNA...
  79. ncbi Human ZP4 is not sufficient for taxon-specific sperm recognition of the zona pellucida in transgenic mice
    Belinda Yauger
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Reproduction 141:313-9. 2011
    ..study sperm-egg recognition, but the mouse zona pellucida surrounding ovulated eggs contains three proteins (ZP1, ZP2, and ZP3) whereas the human zona contains four (ZP1, ZP2, ZP3, and ZP4)...
  80. ncbi Sequence variations in human ZP genes as potential modifiers of zona pellucida architecture
    Reeta Maria Pökkylä
    Department of Obstetrics and Gynecology, University of Oulu, Oulu, Finland
    Fertil Steril 95:2669-72. 2011
    ..Our results suggest that some of the most frequent zona anomalies may be at least partly explained by sequence variations in genes expressing the four human ZP proteins, especially those in ZP2 and ZP3.
  81. ncbi ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida
    Maria Jimenez-Movilla
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 124:940-50. 2011
    The zona pellucida contains three proteins (ZP1, ZP2, ZP3), the precursors of which possess signal peptides, 'zona' domains and short (9-15 residue) cytoplasmic tails downstream of a transmembrane domain...
  82. ncbi Pleiotropic patterning response to activation of Shh signaling in the limb apical ectodermal ridge
    Chi Kuang Leo Wang
    Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, Connecticut, USA
    Dev Dyn 240:1289-302. 2011
    ..Shh expression in the limb is limited to the cells of the zone of polarizing activity (ZPA), located in posterior limb bud mesoderm...
  83. ncbi Identification of spontaneous mutations within the long-range limb-specific Sonic hedgehog enhancer (ZRS) that alter Sonic hedgehog expression in the chicken limb mutants oligozeugodactyly and silkie breed
    Sarah A Maas
    Department of Biomolecular Chemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
    Dev Dyn 240:1212-22. 2011
    The evolutionarily conserved, non-coding ~800-base-pair (bp) zone of polarizing activity (ZPA) regulatory sequence (ZRS) controls Shh expression in the posterior limb...
  84. ncbi Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion
    Kaori Sano
    Graduate Program of Biological Science, Graduate School of Science and Technology, Sophia University, Tokyo, Japan
    FEBS J 275:5934-46. 2008
    ..The six ZHE1-cleaving sites were located in the N-terminal regions of egg envelope subunit proteins, ZP2 and ZP3, but not in the internal regions, such as the ZP domains...
  85. ncbi Adaptive evolution of gamete-recognition proteins in birds
    Sofia Berlin
    Department of Evolutionary Biology, Evolutionary Biology Centre, Uppsala University, Norbyvagen 18 D, 752 36, Uppsala, Sweden
    J Mol Evol 67:488-96. 2008
    ..We therefore sequenced six genes (ZP1, ZP2, ZP4, ZPAX, CD9, and Acrosin) encoding gamete-recognition proteins in several bird species to test for positive ..
  86. ncbi Bonnet monkey (Macaca radiata) ovaries, like human oocytes, express four zona pellucida glycoproteins
    Anasua Ganguly
    Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India
    Mol Reprod Dev 75:156-66. 2008
    ..document that the zona pellucida matrix of human oocytes is composed of four glycoproteins designated as ZP1, ZP2, ZP3, and ZP4 instead of three as proposed in mouse model...
  87. ncbi Phylogenetic analysis and identification of pseudogenes reveal a progressive loss of zona pellucida genes during evolution of vertebrates
    Ghylene Goudet
    Physiologie de la Reproduction et des Comportements, INRA CNRS Université de Tours Haras Nationaux, IFR 135, 37380 Nouzilly, France
    Biol Reprod 78:796-806. 2008
    ..Our study confirmed the classification of ZP genes in six subfamilies: ZPA/ZP2, ZPB/ZP4, ZPC/ZP3, ZP1, ZPAX, and ZPD...
  88. ncbi R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development
    Motoko Aoki
    Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Dev Growth Differ 50:85-95. 2008
    ..Fgf8 and Fgf4 expression in the posterior AER and Sonic hedgehog expression in the zone of polarizing activity (ZPA) were reduced...
  89. ncbi In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes
    Alexandre Kerkis
    Clinica e Centro de Pesquisa em Reproducao Humana Roger Abdelmassih, Sao Paulo, SP, Brasil
    Cloning Stem Cells 9:535-48. 2007
    ..such as Oct-4, Mvh, Stella, Dazl, Piwil 2, Pdrd 1, Rex 14, Rnf 17, Bmp8b, Acrosin, Stra-8, Haprin, LH-R, Gdf9, Zp3, Zp2, Sycp1, and Sycp3...
  90. ncbi Production of monoclonal antibodies against recombinant human zona pellucida glycoproteins: utility in immunolocalization of respective zona proteins in ovarian follicles
    Antonin Bukovsky
    Laboratory of Development, Differentiation and Cancer, The University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Knoxville, TN 37920, USA
    J Reprod Immunol 78:102-14. 2008
    ..stages, murine monoclonal antibodies (MAbs) were generated against the baculovirus-expressed recombinant human ZP2, ZP3 and ZP4...
  91. ncbi Oocytes in newborn MRL mouse testes
    Saori Otsuka
    Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Biol Reprod 79:9-16. 2008
    ..The expression of the oocyte-specific genes Zp1, Zp2, Zp3, and Omt2a was detected in testes from MRL/MpJ mice...
  92. ncbi Antiacrosin antibodies and infertility. II. Gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility
    Carolina Veaute
    Instituto de Biologia y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires, Buenos Aires, Argentina
    Fertil Steril 91:1256-68. 2009
    ..To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility...
  93. ncbi Canine polydactyl mutations with heterogeneous origin in the conserved intronic sequence of LMBR1
    Kiyun Park
    Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu 702 701, Republic of Korea
    Genetics 179:2163-72. 2008
    ..The ZPA regulatory sequence (ZRS) in intron 5 of LMBR1 was implicated in mammalian polydactyly...
  94. ncbi Cellular origin of the Bufo arenarum sperm receptor gp75, a ZP2 family member: its proteolysis after fertilization
    Sonia L Scarpeci
    División Biología del Desarrollo, IBR CONICET UNR and Area Biología, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario S2002RLK, Republic of Argentina
    Biol Cell 100:219-30. 2008
    ..of envelope modification following fertilization is the N-terminal proteolysis of the envelope glycoproteins, ZPA [ZP (zona pellucida) A]...
  95. ncbi Quantitative effects of hedgehog and decapentaplegic activity on the patterning of the Drosophila wing
    P W Ingham
    Molecular Embryology Laboratory, Imperial Cancer Research Fund, London, UK
    Curr Biol 5:432-40. 1995
    ..and expression of the sonic hedgehog (shh) gene is closely associated with the 'zone of polarizing activity' (ZPA) that controls antero-posterior patterning of the vertebrate limb...
  96. ncbi A mathematical model for outgrowth and spatial patterning of the vertebrate limb bud
    R Dillon
    Department of Mathematics, Washington State University, Pullman, WA 99163
    J Theor Biol 197:295-330. 1999
    ..due to cell growth and division, and interactions between morphogens produced in the zone of polarizing activity (ZPA) and the apical epidermal ridge (AER) is developed and analysed...
  97. ncbi Characterization of two zebrafish cDNA clones encoding egg envelope proteins ZP2 and ZP3
    H Wang
    Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochim Biophys Acta 1446:156-60. 1999
    Two zebrafish cDNA clones encoding homologs of mammalian zona pellucida proteins ZP2 and ZP3 were isolated from a whole adult cDNA library. The ZP2 clone encodes a protein of 428 amino acids...
  98. ncbi Structure and function of the proteins of the mammalian Zona pellucida
    S V Prasad
    Department of Cell Biology, Baylor College of Medicine, Houston, Tex, USA
    Cells Tissues Organs 166:148-64. 2000
    ..nomenclature, the ZP proteins from different mammalian species can be classified into three protein families: ZP1, ZP2, and ZP3...
  99. ncbi The mouse zona pellucida: folliculogenesis, fertility and pre-implantation development
    T Rankin
    Laboratory of Cellular and Developmental Biology, NIDDK, Building 6, Room B1 26, National Institutes of Health, 6 Center DR MSC 2715, Bethesda, MD 20892, USA
    Mol Cell Endocrinol 163:21-5. 2000
    ..In mice, the zona matrix is composed of three proteins (ZP1, ZP2, ZP3). Mouse lines lacking either ZP1 or ZP3 have been established and have abnormal folliculogenesis...
  100. ncbi FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation
    S M Soyal
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Development 127:4645-54. 2000
    ..The additional observation that null females do not express Zp1, Zp2 or Zp3 indicates that FIGalpha plays a key regulatory role in the expression of multiple oocyte-specific genes, ..
  101. ncbi The ovary as an immune target
    B S Dunbar
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Soc Gynecol Investig 8:S43-8. 2001
    ..have shown that immunization of primates with ZP proteins expressed from cDNAs coding for the mouse and rabbit ZP2 (the mouse homologue has 60% amino acid identity with human ZP2) or the mouse ZP3 (the mouse protein has 67% amino ..

Research Grants62

  1. OOCYTE CORTICAL MATURATION AND GRANULE ACTIVATION
    THOMAS DUCIBELLA; Fiscal Year: 1993
    ..will be assayed by % CG loss [quantitative image analysis(QIA)], by biochemical analysis of the zona (protein ZP2), and by % monospermic (vs polyspermic) fertilization. 2...
  2. Gap Junctions/Limb Outgrowth, Patterning, Skeletogenesis
    CAROLINE DEALY; Fiscal Year: 2004
    ..directs the proliferation and proximodistal outgrowth of the limb mesoderm, and the zone of polarizing activity (ZPA), a group of mesoderm cells located at the posterior periphery of the limb which is thought to specify positional ..
  3. A novel regulator of PI-3-Kinase in human liver cancer
    Marie DeFrances; Fiscal Year: 2007
    ..Recently, we discovered a novel kringle containing transmembrane protein we call zPA that harbors a p85-1ike domain in its C-terminal, intracellular portion...
  4. VERTEBRATE ORGANIZER FORMATION CELL AND MOLECULAR CUES
    Lee Niswander; Fiscal Year: 2000
    ..centers that direct patterning of the three axes, the apical ectodermal ridge, posterior polarizing mesenchyme (ZPA), and non- ridge ectoderm...
  5. Regulation of Shh Signaling Activity in Limb Patterning
    Chin Chiang; Fiscal Year: 2007
    ..vertebrate limbs, where Shh, normally expressed in the posterior limb margin of the zone of polarizing activity (ZPA), has ability to progressively specify increase in number of digits with more posterior identities in a dose-..
  6. NOTCH SIGNALING AND VERTEBRATE LIMB DEVELOPMENT
    Juan Carlos Izpisua Belmonte; Fiscal Year: 2003
    ..the Hensen's node, the midbrain-hindbrain junction or, in the case of the limb, the zone of polarizing activity (ZPA) of the apical ectodermal ridge (AER)...
  7. Regulation of Shh Signaling Activity in Limb Patterning
    Chin Chiang; Fiscal Year: 2010
    ..vertebrate limbs, where Shh, normally expressed in the posterior limb margin of the zone of polarizing activity (ZPA), has ability to progressively specify increase in number of digits with more posterior identities in a dose-..
  8. EPITHELIAL/MESENCHYMAL INTERACTIONS IN LIMB PATTERNING
    John Fallon; Fiscal Year: 2009
    The long-term goal of this research is to understand how the zone of polarizing activity (ZPA) functions in space and developmental time to control anterior/posterior (A/P) limb patterning...
  9. SONIC HEDGEHOG'S ROLE IN PATTERNING THE LIMB
    Clifford Tabin; Fiscal Year: 1999
    ..Examples of such centers include the ZPA which organizes the anterior-posterior axis of the limb and the notochord and floor plate of the neural tube which ..
  10. A Lineage Analysis of Cells in the Vertebrate Limb
    JENNIFER MANSFIELD; Fiscal Year: 2005
    ..Second, the progeny of cells in the Zone of Polarizing Activity (ZPA) will be traced...
  11. MECHANISMS OF MAMMALIAN FERTILIZATION
    PAUL WASSARMAN; Fiscal Year: 2006
    ..Furthermore, the research could have important consequences for development of novel reversible contraceptive methods that prevent ZP formation during oogenesis in humans. ..
  12. MECHANISMS OF MAMMALIAN FERTILIZATION
    PAUL WASSARMAN; Fiscal Year: 2007
    ..Furthermore, the research could have important consequences for development of novel reversible contraceptive methods that prevent ZP formation during oogenesis in humans. ..
  13. MECHANISMS OF MAMMALIAN FERTILIZATION
    PAUL WASSARMAN; Fiscal Year: 2001
    ..In view of the relatedness of mouse and human ZP3 it is anticipated that results of this investigation will impact significantly on our understanding of fundamental aspects of fertilization in human beings. ..
  14. Proteomics of Egg Cortical Granules
    LEANN LINDSAY; Fiscal Year: 2003
    ..This approach will identify potential molecules important in egg extracellular matrix modifications, which will be the subject of future investigations. ..
  15. MECHANISMS OF MAMMALIAN FERTILIZATION
    PAUL WASSARMAN; Fiscal Year: 2003
    ..Furthermore, the research could have important consequences for development of novel reversible contraceptive methods that prevent ZP formation during oogenesis in humans. ..
  16. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2007
    ....
  17. GENE THERAPY APPROACH FOR ENGINEERING CRANIOFACIAL BONE
    Renny Franceschi; Fiscal Year: 2004
    ..These studies will greatly advance our understanding of BMP-dependent bone regeneration and develop a viable gene therapy approach for regenerating bone in the craniofacial and appendicular skeleton. ..
  18. Identify and characterize novel Wnt pathway genes
    Brian Harfe; Fiscal Year: 2006
    ..The PIs have experience working with C. elegans, mice and chick model systems and are positioned to take full advantage of each system. ..