Gene Symbol: ZBRK1
Description: zinc finger protein 350
Alias: ZBRK1, ZFQR, zinc finger protein 350, KRAB zinc finger protein ZFQR, zinc finger and BRCA1-interacting protein with a KRAB domain 1, zinc finger protein ZBRK1
Species: human
Products:     ZBRK1

Top Publications

  1. Lin L, Chuang C, Li C, Liao C, Cheng C, Cheng T, et al. ZBRK1 acts as a metastatic suppressor by directly regulating MMP9 in cervical cancer. Cancer Res. 2010;70:192-201 pubmed publisher
    The BRCA1-interacted transcriptional repressor ZBRK1 has been associated with antiangiogenesis, but direct evidence of a tumor suppressor role has been lacking. In this study, we provide evidence of such a role in cervical carcinoma...
  2. Yun J, Lee W. Degradation of transcription repressor ZBRK1 through the ubiquitin-proteasome pathway relieves repression of Gadd45a upon DNA damage. Mol Cell Biol. 2003;23:7305-14 pubmed
    ..Previously, we identified a novel zinc finger protein, ZBRK1, which contains a KRAB domain at the N terminus, eight zinc fingers at the center, and a BRCA1-binding region at ..
  3. Rutter J, Smith A, Dávila M, Sigurdson A, Giusti R, Pineda M, et al. Mutational analysis of the BRCA1-interacting genes ZNF350/ZBRK1 and BRIP1/BACH1 among BRCA1 and BRCA2-negative probands from breast-ovarian cancer families and among early-onset breast cancer cases and reference individuals. Hum Mutat. 2003;22:121-8 pubmed
    Two potential breast cancer susceptibility genes, encoding the BRCA1-interacting proteins ZNF350 (or ZBRK1) and BRIP1 (or BACH1), have been identified in yeast two-hybrid screens...
  4. Furuta S, Wang J, Wei S, Jeng Y, Jiang X, Gu B, et al. Removal of BRCA1/CtIP/ZBRK1 repressor complex on ANG1 promoter leads to accelerated mammary tumor growth contributed by prominent vasculature. Cancer Cell. 2006;10:13-24 pubmed
    BRCA1 exerts transcriptional repression through interaction with CtIP in the C-terminal BRCT domain and ZBRK1 in the central domain...
  5. Zheng L, Pan H, Li S, Flesken Nikitin A, Chen P, Boyer T, et al. Sequence-specific transcriptional corepressor function for BRCA1 through a novel zinc finger protein, ZBRK1. Mol Cell. 2000;6:757-68 pubmed
    ..To explore the mechanistic basis for this regulation, a novel human gene, ZBRK1, which encodes a 60 kDa protein with an N-terminal KRAB domain and eight central zinc fingers, was identified by ..
  6. Lee Y, Thomas S, Yang A, Ann D. Doxorubicin down-regulates Kruppel-associated box domain-associated protein 1 sumoylation that relieves its transcription repression on p21WAF1/CIP1 in breast cancer MCF-7 cells. J Biol Chem. 2007;282:1595-606 pubmed
    ..cofactor KRAB domain-associated protein 1 (KAP1) has been shown to complex with the KRAB zinc finger protein, ZBRK1, to repress the transcription of target genes...
  7. Liao G, Huang J, Fixman E, Hayward S. The Epstein-Barr virus replication protein BBLF2/3 provides an origin-tethering function through interaction with the zinc finger DNA binding protein ZBRK1 and the KAP-1 corepressor. J Virol. 2005;79:245-56 pubmed
    ..The screen also identified the DNA binding zinc finger protein ZBRK1 and the ZBRK1 corepressor KAP-1 as BBLF2/3 interactors...
  8. Groner A, Meylan S, Ciuffi A, Zangger N, Ambrosini G, Dénervaud N, et al. KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. PLoS Genet. 2010;6:e1000869 pubmed publisher
    ..This finding not only suggests auto-regulatory mechanisms in the control of KRAB-ZFP gene clusters, but also provides important cues for interpreting future genome-wide DNA binding data of KRAB-ZFPs and KAP1. ..
  9. Li H, Seth A. An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein. Oncogene. 2004;23:1801-8 pubmed
    ..Among 18 in-frame positive clones, three were found to be ZBRK1, Eps15 and AMSH (associated molecule with the SH3 domain of STAM)...

More Information


  1. Garcia Closas M, Egan K, Newcomb P, Brinton L, Titus Ernstoff L, Chanock S, et al. Polymorphisms in DNA double-strand break repair genes and risk of breast cancer: two population-based studies in USA and Poland, and meta-analyses. Hum Genet. 2006;119:376-88 pubmed
    ..Evaluation of potential underlying gene-gene interactions or associations in population subgroups will require even larger sample sizes. ..
  2. Bulliard Y, Wiznerowicz M, Barde I, Trono D. KRAB can repress lentivirus proviral transcription independently of integration site. J Biol Chem. 2006;281:35742-6 pubmed
    ..These results indicate that KRAB-induced transcriptional repression is robust and active over a variety of genomic contexts that include at least the wide range of sites targeted by lentiviral integration. ..
  3. Tan W, Zheng L, Lee W, Boyer T. Functional dissection of transcription factor ZBRK1 reveals zinc fingers with dual roles in DNA-binding and BRCA1-dependent transcriptional repression. J Biol Chem. 2004;279:6576-87 pubmed
    ..To clarify this issue, we have examined the functional interaction between BRCA1 and ZBRK1, a BRCA1-dependent KRAB eight zinc finger transcriptional repressor...
  4. Xiao T, Suh Y, Longley B. MAGE proteins regulate KRAB zinc finger transcription factors and KAP1 E3 ligase activity. Arch Biochem Biophys. 2014;563:136-44 pubmed publisher
    ..MAGE-A3 has no significant impact on KZNFs with KRAB domains containing the Scan box motif. These data support our hypothesis by showing that the effects of MAGE-A3 on gene repression depend on the type of KZNF KRAB domain involved. ..
  5. Liao C, Tsai C, Chang W, Lee W, Wang J. RB·E2F1 complex mediates DNA damage responses through transcriptional regulation of ZBRK1. J Biol Chem. 2010;285:33134-43 pubmed publisher
    ..We examined whether RB can mediate the response to DNA damage through modulation of ZBRK1, a zinc finger-containing transcriptional repressor that can modulate the expression of GADD45A, a DNA damage ..
  6. Huo X, Lu C, Huang X, Hu Z, Jin G, Ma H, et al. Polymorphisms in BRCA1, BRCA1-interacting genes and susceptibility of breast cancer in Chinese women. J Cancer Res Clin Oncol. 2009;135:1569-75 pubmed publisher
    BRCA1-interacting protein C-terminal helicase 1 (BRIP1) and zinc finger protein 350 (ZNF350) work with BRCA1 in tumor suppression procedures...
  7. Tan W, Kim S, Boyer T. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated box-zinc finger protein ZBRK1. J Biol Chem. 2004;279:55153-60 pubmed
    The Kruppel-associated box (KRAB)-zinc finger protein ZBRK1 has been implicated in the transcriptional regulation of DNA damage-response genes that function in cell growth control and survival...
  8. Meylan S, Groner A, Ambrosini G, Malani N, Quenneville S, Zangger N, et al. A gene-rich, transcriptionally active environment and the pre-deposition of repressive marks are predictive of susceptibility to KRAB/KAP1-mediated silencing. BMC Genomics. 2011;12:378 pubmed publisher
    ..Together, these data indicate that high levels of gene activity in the genomic environment and the pre-deposition of repressive histone marks within a gene increase its susceptibility to KRAB/KAP1-mediated repression. ..
  9. Barde I, Laurenti E, Verp S, Groner A, Towne C, Padrun V, et al. Regulation of episomal gene expression by KRAB/KAP1-mediated histone modifications. J Virol. 2009;83:5574-80 pubmed publisher
  10. Chen K, Yu G, Gumireddy K, Li A, Yao W, Gao L, et al. ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer. Oncotarget. 2015;6:6959-76 pubmed
    ..In the present study, we identified ZBRK1 as a novel VHL interacting protein by yeast two-hybrid screening, and found a single ZBRK1-binding site located in ..
  11. Gu S, Rong H, Zhang G, Kang L, Yang M, Guan H. Functional SNP in 3'-UTR MicroRNA-Binding Site of ZNF350 Confers Risk for Age-Related Cataract. Hum Mutat. 2016;37:1223-1230 pubmed publisher
    ..These results suggested that the association of SNP rs2278414 with ARC might involve an altered miRNA regulation of ZNF350. ..
  12. Hallen L, Klein H, Stoschek C, Wehrmeyer S, Nonhoff U, Ralser M, et al. The KRAB-containing zinc-finger transcriptional regulator ZBRK1 activates SCA2 gene transcription through direct interaction with its gene product, ataxin-2. Hum Mol Genet. 2011;20:104-14 pubmed publisher
    ..Here, we report the identification of the KRAB-containing zinc-finger transcriptional regulator, ZBRK1, as an interaction partner of the SCA2 gene product ataxin-2...
  13. Patnaik S, George S, Pham E, Roy S, Singh K, Mariadason J, et al. By moonlighting in the nucleus, villin regulates epithelial plasticity. Mol Biol Cell. 2016;27:535-48 pubmed publisher
    ..we find that villin directly interacts with a transcriptional corepressor and ligand of the Slug promoter, ZBRK1. The outcome of this study underscores the role of nuclear villin and its binding partner ZBRK1 in the regulation ..
  14. Nishitsuji H, Abe M, Sawada R, Takaku H. ZBRK1 represses HIV-1 LTR-mediated transcription. FEBS Lett. 2012;586:3562-8 pubmed publisher
    ..We show that ZBRK1 negatively regulates the HIV-1 LTR...
  15. Ran Q, Wadhwa R, Bischof O, Venable S, Smith J, Pereira Smith O. Characterization of a novel zinc finger gene with increased expression in nondividing normal human cells. Exp Cell Res. 2001;263:156-62 pubmed
    We report here the cloning and characterization of a novel KRAB zinc finger gene, ZFQR, which has eight tandemly repeated zinc fingers, a complete KRAB box at the N-terminal region, and a unique C-terminal sequence...
  16. Lin L, Li C, Wang W, Yang W, Wang D, Chang W, et al. Loss of ZBRK1 contributes to the increase of KAP1 and promotes KAP1-mediated metastasis and invasion in cervical cancer. PLoS ONE. 2013;8:e73033 pubmed publisher
    b>ZBRK1, a zinc finger protein that interacts with breast cancer 1 (BRCA1) and KRAB-ZFP-associated protein 1 (KAP1), has been suggested to serve as a tumor suppressor via repression of tumor metastasis/invasion...
  17. Wang W, Cai J, Wu Y, Hu L, Chen Z, Hu J, et al. Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1. Cell Mol Life Sci. 2013;70:3947-58 pubmed publisher
    ..Our study also suggests that loss of the KRAB domain in KRAB-ZFPs due to aberrant alternative splicing might contribute to carcinogenesis. ..
  18. Plourde K, Labrie Y, Desjardins S, Belleau P, Ouellette G, Durocher F. Analysis of ZNF350/ZBRK1 promoter variants and breast cancer susceptibility in non-BRCA1/2 French Canadian breast cancer families. J Hum Genet. 2013;58:59-66 pubmed publisher
    ZNF350/ZBRK1 is a transcription factor, which associates with BRCA1 to co-repress GADD45A to regulate DNA damage repair, and the expression of ZNF350 is altered in different human carcinomas...
  19. Yarden R, Friedman E, Metsuyanim S, Olender T, Ben Asher E, Papa M. Single-nucleotide polymorphisms in the p53 pathway genes modify cancer risk in BRCA1 and BRCA2 carriers of Jewish-Ashkenazi descent. Mol Carcinog. 2010;49:545-55 pubmed publisher
    ..genes that affect p53-mediated apoptosis, as well as two tag-SNPs in the CHEK2 (C>T rs743184) and the ZBRK1/ZNF350 (G>A rs2278414) genes that encode for proteins involved in growth arrest following DNA damage...
  20. Ahmed K, Tsai C, Lee W. Derepression of HMGA2 via removal of ZBRK1/BRCA1/CtIP complex enhances mammary tumorigenesis. J Biol Chem. 2010;285:4464-71 pubmed publisher
    ..Using microarray analysis, we found that HMGA2, along with a dozen of other genes, was co-repressed by ZBRK1, BRCA1, and CtIP...
  21. Yadav L, Biswal M, Vikrant -, Hosur M, Varma A. Tetrameric ZBRK1 DNA binding domain has affinity towards cognate DNA in absence of zinc ions. Biochem Biophys Res Commun. 2014;450:283-8 pubmed publisher
    ..Human ZBRK1 is a zinc-finger transcription repressor protein, which recognizes double helical DNA containing consensus ..
  22. Desjardins S, Belleau P, Labrie Y, Ouellette G, Bessette P, Chiquette J, et al. Genetic variants and haplotype analyses of the ZBRK1/ZNF350 gene in high-risk non BRCA1/2 French Canadian breast and ovarian cancer families. Int J Cancer. 2008;122:108-16 pubmed
    ..to identify new genes potentially involved in breast cancer susceptibility, we analyzed genomic variants in the ZBRK1 gene, a co-repressor implicated in BRCA1-mediated repression of GADD45...
  23. Figueroa J, Malats N, Rothman N, Real F, Silverman D, Kogevinas M, et al. Evaluation of genetic variation in the double-strand break repair pathway and bladder cancer risk. Carcinogenesis. 2007;28:1788-93 pubmed
    ..17 (1.00-1.36). Results from this study provide evidence for associations between variants in genes in the DSBR pathway and bladder cancers risk that warrant replication in other study populations. ..
  24. Sigurdson A, Hauptmann M, Chatterjee N, Alexander B, Doody M, Rutter J, et al. Kin-cohort estimates for familial breast cancer risk in relation to variants in DNA base excision repair, BRCA1 interacting and growth factor genes. BMC Cancer. 2004;4:9 pubmed
    ..R280H; IGFPB3 -202A>C; TGFss1 L10P, P25R, and T263I; BRCA2 N289H and T1915M; BRIP1 -64A>C; and ZNF350 (or ZBRK1) 1845C>T, L66P, R501S, and S472P...
  25. Surpili M, Delben T, Kobarg J. Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1. Biochemistry. 2003;42:15369-76 pubmed
    ..Our results give further support to the previous observation that NEK1 is of functional importance for the etiology of PKD. ..
  26. Heride C, Rigden D, Bertsoulaki E, Cucchi D, De Smaele E, Clague M, et al. The centrosomal deubiquitylase USP21 regulates Gli1 transcriptional activity and stability. J Cell Sci. 2016;129:4001-4013 pubmed
    ..By revealing an intriguing functional pairing between a spatially restricted deubiquitylase and a kinase, our study highlights the centrosome as an important hub for signal coordination. ..