XPO1

Summary

Gene Symbol: XPO1
Description: exportin 1
Alias: CRM1, emb, exp1, exportin-1, chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, exportin 1 (CRM1 homolog, yeast), exportin-1 (required for chromosome region maintenance)
Species: human

Top Publications

  1. pmc Transcriptional and posttranscriptional regulation of HIV-1 gene expression
    Jonathan Karn
    Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Cold Spring Harb Perspect Med 2:a006916. 2012
  2. doi Inhibition of CRM1-mediated nucleocytoplasmic transport: triggering human melanoma cell apoptosis by perturbing multiple cellular pathways
    Gaurav Pathria
    Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria
    J Invest Dermatol 132:2780-90. 2012
  3. pmc A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export
    Thomas Wild
    Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
    PLoS Biol 8:e1000522. 2010
  4. ncbi Cessation of HIV-1 transcription by inhibiting regulatory protein Rev-mediated RNA transport
    Yuan Cao
    Institute of Medicinal Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan, P R China
    Curr HIV Res 7:101-8. 2009
  5. pmc Importin 7 and exportin 1 link c-Myc and p53 to regulation of ribosomal biogenesis
    Lior Golomb
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 45:222-32. 2012
  6. pmc The structural biology of HIV-1: mechanistic and therapeutic insights
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Nat Rev Microbiol 10:279-90. 2012
  7. pmc The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
    A Bachi
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 6:136-58. 2000
  8. ncbi Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors
    A Kiss
    Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
    Virology 314:591-600. 2003
  9. ncbi Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7
    Yoshitaka Tajima
    Department of Biochemistry, The Cancer Institute of the Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170 8455, Japan
    J Biol Chem 278:10716-21. 2003
  10. doi Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteins
    Supratik Das
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 485:271-93. 2009

Research Grants

  1. Cyclin D1 and mammary carcinoma
    J Diehl; Fiscal Year: 2009
  2. Lizabeth A Allison; Fiscal Year: 2015
  3. Yuh Min Chook; Fiscal Year: 2016
  4. How Karyopherins Move Across The Nuclear Pore Complex
    Michael Rexach; Fiscal Year: 2006
  5. HTS Assay Development for Modulators of a Nuclear Import/Export Switch
    ISABELLA GRAEF; Fiscal Year: 2007
  6. Screen for Small Molecule Inhibitors of Nuclear Protein Import
    Larry Gerace; Fiscal Year: 2007
  7. Katherine L B Borden; Fiscal Year: 2016
  8. Nuclear export of the HIV genome: A Molecular Dynamics Study of the Interactions
    MOHAMMAD RK MOFRAD; Fiscal Year: 2013
  9. MOLECULAR CHARACTERIZATION OF HERPES SIMPLEX VIRUS ICP27
    Stephen Rice; Fiscal Year: 2003
  10. Structural requirements for the nuclear export of HIV RNA
    DAVID SCOTT BOOTH; Fiscal Year: 2013

Detail Information

Publications234 found, 100 shown here

  1. pmc Transcriptional and posttranscriptional regulation of HIV-1 gene expression
    Jonathan Karn
    Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Cold Spring Harb Perspect Med 2:a006916. 2012
    ..In cells that are not fully activated, limiting levels of Tat and Rev act as potent blocks to premature virus production...
  2. doi Inhibition of CRM1-mediated nucleocytoplasmic transport: triggering human melanoma cell apoptosis by perturbing multiple cellular pathways
    Gaurav Pathria
    Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria
    J Invest Dermatol 132:2780-90. 2012
    ..DNA microarray analysis, we identified four key components of the nucleocytoplasmic transport machinery-CRM1, RAN (RAN-GTPase), RANGAP1, and RANBP1-to be overexpressed in human melanoma metastases...
  3. pmc A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export
    Thomas Wild
    Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
    PLoS Biol 8:e1000522. 2010
    ..We show that Exp5, like the known 60S exportin Crm1, binds to pre-60S particles in a RanGTP-dependent manner...
  4. ncbi Cessation of HIV-1 transcription by inhibiting regulatory protein Rev-mediated RNA transport
    Yuan Cao
    Institute of Medicinal Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan, P R China
    Curr HIV Res 7:101-8. 2009
    ..and cytoplasm, facilitates export of unspliced and singly spliced viral transcripts containing RRE RNA by the CRM1 export pathway...
  5. pmc Importin 7 and exportin 1 link c-Myc and p53 to regulation of ribosomal biogenesis
    Lior Golomb
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 45:222-32. 2012
    ..We report that transcription of the β-karyopherin genes importin 7 (IPO7) and exportin 1 (XPO1), and several additional nuclear import receptors, is regulated positively by c-Myc and negatively by p53...
  6. pmc The structural biology of HIV-1: mechanistic and therapeutic insights
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Nat Rev Microbiol 10:279-90. 2012
    ..Here, we review recent advances in HIV-1 structural biology, focusing on the molecular mechanisms of viral replication and on the development of new therapeutics...
  7. pmc The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
    A Bachi
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 6:136-58. 2000
    ....
  8. ncbi Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors
    A Kiss
    Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
    Virology 314:591-600. 2003
    ..These results suggest a physiological role for hNup98 in modulating Rev-dependent RNA export during HIV infection...
  9. ncbi Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7
    Yoshitaka Tajima
    Department of Biochemistry, The Cancer Institute of the Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170 8455, Japan
    J Biol Chem 278:10716-21. 2003
    ..In transfected cells, the Smurf1-Smad7 complex was accumulated in the cytoplasm by the nuclear export receptor, CRM1; this action was prevented by treatment with leptomycin B, a specific inactivator of CRM1 function...
  10. doi Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteins
    Supratik Das
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 485:271-93. 2009
    ..Once the mutants are isolated, they are tested in other biochemical systems and can be subjected to further functional and virological studies...
  11. pmc Identification of poly (ADP-ribose) polymerase-1 (PARP-1) as a novel Kruppel-like factor 8-interacting and -regulating protein
    Heng Lu
    Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida 32827, USA
    J Biol Chem 286:20335-44. 2011
    ..This mislocalization was prevented by either PARP-1 re-expression or inhibition of CRM1-dependent nuclear export. Interestingly, co-IP indicated competition between PARP-1 and CRM1 for KLF8 binding...
  12. pmc Nuclear-to-cytoplasmic relocalization of the proliferating cell nuclear antigen (PCNA) during differentiation involves a chromosome region maintenance 1 (CRM1)-dependent export and is a prerequisite for PCNA antiapoptotic activity in mature neutrophils
    Dikra Bouayad
    Inserm U1016, 75014 Paris, France
    J Biol Chem 287:33812-25. 2012
    ..We showed here that leptomycin B, an inhibitor of the chromosome region maintenance 1 (CRM1) exportin, inhibited PCNA relocalization during granulocytic differentiation of HL-60 and NB4 promyelocytic cell ..
  13. ncbi Active nuclear import and export pathways regulate E2F-5 subcellular localization
    Margarita D Apostolova
    Department of Physiology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 277:34471-9. 2002
    ..Indeed, E2F-5 is exported from the nucleus through leptomycin B-sensitive, CRM1-mediated transport, through a region corresponding to amino acid residues 130-154...
  14. pmc Epstein-Barr virus LMP1 blocks p16INK4a-RB pathway by promoting nuclear export of E2F4/5
    Naoko Ohtani
    Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, UK
    J Cell Biol 162:173-83. 2003
    ..Here, we report that LMP1 promotes the CRM1-dependent nuclear export of Ets2, which is an important transcription factor for p16INK4a gene expression, thereby ..
  15. ncbi A CRM1-dependent nuclear export pathway is involved in the regulation of IRF-5 subcellular localization
    Rongtuan Lin
    Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Biol Chem 280:3088-95. 2005
    ..In the present study, we demonstrate that a CRM1-dependent nuclear export pathway is involved in the regulation of IRF-5 subcellular localization...
  16. pmc Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants
    A M Szilvay
    National Centre for Research in Virology, Gade Institute, University of Bergen, Norway
    J Virol 69:3315-23. 1995
    ..In cotransfection experiments, mutants containing mutations of Rev inhibited the nuclear egress of wild-type Rev, thus revealing a novel transdominant negative phenotype...
  17. ncbi Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1
    A S Zolotukhin
    Human Retrovirus Pathogenesis Group, NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
    J Biol Chem 272:11356-60. 1997
    ..These data suggest that RanBP1 interacts with Rev at the putative nuclear receptor and, hence, shares a step in posttranscriptional pathway with Rev...
  18. ncbi A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1
    G Farjot
    Laboratoire de Virologie, ENS Lyon, Unité INSERM U 412, 46, Allee d Italie, 69364 Lyon Cedex 07, France
    J Biol Chem 274:17309-17. 1999
    ..We show here that NLP-1, like the previously described Rev-interacting protein hRIP/Rab and several nucleoporins, also interacts with CRM-1 both in yeast and mammalian cells...
  19. ncbi The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1
    B B Singh
    Department of Pharmacology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 274:37370-8. 1999
    ..Here, we show the zinc fingers of RanBP2 associate with high specificity to the nuclear export factor, exportin-1 (CRM1)...
  20. ncbi Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor function
    X H Liang
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Cancer Res 61:3443-9. 2001
    ..signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport...
  21. ncbi Alternative splicing of Staufen2 creates the nuclear export signal for CRM1 (Exportin 1)
    Takashi Miki
    Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Japan
    J Biol Chem 279:47473-9. 2004
    ..from the nucleus by two distinct pathways, one of which is RBD3-mediated and the other of which is CRM1 (exportin 1)-dependent...
  22. ncbi Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanism
    Shipra Rastogi
    Drug Discovery Program, Department of Interdisciplinary Oncology, H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
    J Biol Chem 281:2951-9. 2006
    ..These results suggest that the regulated translocation of prohibitin from the nucleus to the mitochondria facilitates its pleiotropic functions and might contribute to its anti-proliferative and tumor suppressive properties...
  23. ncbi Differential IFN-gamma stimulation of HLA-A gene expression through CRM-1-dependent nuclear RNA export
    Sarah K Browne
    Massey Cancer Center, Virginia Commonwealth University Medical Center, 401 College Street, Richmond, VA 23298, USA
    J Immunol 177:8612-9. 2006
    ....
  24. ncbi Modulating HIV-1 RNA processing and utilization
    Meredith McLaren
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario
    Front Biosci 13:5693-707. 2008
    ..The observations made underline the multiple fate decisions that must be made at each stage of the viral RNA metabolic pathway and highlight potential new avenues for controlling HIV-1 replication...
  25. doi Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Virus Genes 40:341-6. 2010
    ..Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death.
  26. doi Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-α 1 mRNA
    Tominori Kimura
    Laboratory of Microbiology and Cell Biology, Department of Pharmacy, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga 525 8577, Japan
    Med Mol Morphol 43:145-57. 2010
    We recently reported the chromosome region maintenance 1 (CRM1)-dependent nuclear export of intron-less human interferon-α1 (IFN-α1) mRNA, which encodes a main effecter of host innate immunity...
  27. ncbi Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1
    O Rosorius
    Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Schlossgarten 4, D 91054 Erlangen, Germany
    J Cell Sci 112:2369-80. 1999
    ..We are able to show that eIF-5A interacts with the general nuclear export receptor, CRM1. Furthermore, microinjection studies in somatic cells revealed that eIF-5A is transported from the nucleus to the ..
  28. pmc Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1
    K Plafker
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 20:3510-21. 2000
    ..Shuttling of RanBP1 may function to clear nuclear pores of Ran:GTP, to prevent premature release of import cargo from transport receptors...
  29. pmc Nuclear targeting of adenovirus type 2 requires CRM1-mediated nuclear export
    Sten Strunze
    University of Zurich, Institute of Zoology, CH 8057 Zurich, Switzerland
    Mol Biol Cell 16:2999-3009. 2005
    ..Two distinct inhibitors of the nuclear export factor CRM1, leptomycin B (LMB) and ratjadone A (RJA) or CRM1-siRNAs blocked adenovirus infection, arrested cytoplasmic ..
  30. pmc Endosomal transport of ErbB-2: mechanism for nuclear entry of the cell surface receptor
    Dipak K Giri
    Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, 77030, USA
    Mol Cell Biol 25:11005-18. 2005
    ..This pathway may serve as a general mechanism to allow direct communication between cell surface receptors and the nucleus, and our findings thus open a new era in understanding direct trafficking between the cell membrane and nucleus...
  31. pmc Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport
    F R Bischoff
    Division of Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    Proc Natl Acad Sci U S A 92:1749-53. 1995
    ..Thus, a defect in either of the two antagonistic regulators of Ran would result in a shutdown of Ran-dependent transport processes, in agreement with the almost identical phenotypes described for such defects in budding yeast...
  32. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
    b>CRM1 is distantly related to receptors that mediate nuclear protein import and was previously shown to interact with the nuclear pore complex. Overexpression of CRM1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus...
  33. pmc CRM1-mediated recycling of snurportin 1 to the cytoplasm
    E Paraskeva
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 145:255-64. 1999
    ..We have shown previously that CAS mediates export of importin alpha. Here we show that snurportin 1 is exported by CRM1, the receptor for leucine-rich nuclear export signals (NESs)...
  34. pmc An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein
    J Yang
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 96:13404-8. 1999
    ..Like HIV-1 Rev, K-Rev binds to both the Crm1 nuclear export factor and to a cis-acting viral RNA target to activate nuclear export of unspliced RNAs...
  35. ncbi Transport between the cell nucleus and the cytoplasm
    D Gorlich
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany
    Annu Rev Cell Dev Biol 15:607-60. 1999
    ..We discuss mechanistic aspects and the energetics of transport receptor function and describe a number of pathways in detail...
  36. ncbi Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway
    Franziska Thomas
    Swiss Federal Institute of Technology ETH Zurich, Institute of Biochemistry, HPM F11 1, Switzerland
    J Cell Sci 116:2409-19. 2003
    ..Most nuclear export pathways require RanGTP-binding export receptors. We analyzed the role of CRM1, the export receptor for leucine-rich nuclear export signals (NES), in the biogenesis of ribosomal subunits in ..
  37. pmc Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates
    Christopher R Trotta
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA
    EMBO J 22:2841-51. 2003
    ..We show that in vertebrate cells, transport of both subunits requires the export receptor CRM1 and Ran.GTP. Export of 60S subunits is coupled with that of the nucleo- cytoplasmic shuttling protein NMD3...
  38. ncbi Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex
    Carlo Petosa
    European Molecular Biology Laboratory, Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
    Mol Cell 16:761-75. 2004
    b>CRM1/Exportin1 mediates the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran...
  39. ncbi The expression of CRM1 is associated with prognosis in human osteosarcoma
    Yang Yao
    Department of Oncology, Affiliated 6th People s Hospital, Shanghai Jiaotong University, Shanghai 200223, P R China
    Oncol Rep 21:229-35. 2009
    The nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) is involved in the nuclear export of proteins and messenger RNAs and, thus, mediates the subcellular distribution of important molecules...
  40. doi The Karyopherin proteins, Crm1 and Karyopherin beta1, are overexpressed in cervical cancer and are critical for cancer cell survival and proliferation
    Pauline J van der Watt
    Division of Medical Biochemistry, Faculty of Health Sciences, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa
    Int J Cancer 124:1829-40. 2009
    ..In this study, we examined the expression of the Karyopherins, Crm1, Karyopherin beta1 (Kpnbeta1) and Karyopherin alpha2 (Kpnalpha2), in cervical tissue and cell lines...
  41. pmc Structural basis for leucine-rich nuclear export signal recognition by CRM1
    Xiuhua Dong
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390 9041, USA
    Nature 458:1136-41. 2009
    CRM1 (also known as XPO1 and exportin 1) mediates nuclear export of hundreds of proteins through the recognition of the leucine-rich nuclear export signal (LR-NES). Here we present the 2.9 A structure of CRM1 bound to snurportin 1 (SNUPN)...
  42. doi Active nuclear import and cytoplasmic retention of activation-induced deaminase
    Anne Marie Patenaude
    Institut de Recherches Cliniques de Montreal, Quebec, Canada
    Nat Struct Mol Biol 16:517-27. 2009
    ..Blocking nuclear import alters the balance between these processes in favor of cytoplasmic retention, resulting in reduced isotype class switching...
  43. pmc Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages
    Hiroyuki Okada
    Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
    Retrovirology 6:43. 2009
    ..basis for developing a better rat model for HIV-1 infection, we evaluated the effect of human CyclinT1 (hCycT1) and CRM1 (hCRM1) on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells ..
  44. doi Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase
    Tominori Kimura
    Laboratory of Microbiology and Cell Biology, Department of Pharmacy, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga, 525 8577, Japan
    Med Mol Morphol 42:70-81. 2009
    ..The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export...
  45. doi Expression of CRM1 in human gliomas and its significance in p27 expression and clinical prognosis
    Aiguo Shen
    The Jiangsu Province Key Lab of Neuroregeneration, Nantong University, Nantong, China
    Neurosurgery 65:153-9; discussion 159-60. 2009
    ..It is believed that phosphorylation of p27 on Ser10 has been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
  46. pmc An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1
    Masako Koyama
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya, Japan
    EMBO J 29:2002-13. 2010
    The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES)...
  47. pmc Electrostatic interactions involving the extreme C terminus of nuclear export factor CRM1 modulate its affinity for cargo
    Abigail M Fox
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    J Biol Chem 286:29325-35. 2011
    The toroid-shaped nuclear protein export factor CRM1 is constructed from 21 tandem HEAT repeats, each of which contains an inner (B) and outer (A) α-helix joined by loops...
  48. pmc Prognostic impact and targeting of CRM1 in acute myeloid leukemia
    Kensuke Kojima
    Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Blood 121:4166-74. 2013
    Chromosomal region maintenance 1 (CRM1) is a nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal. CRM1 is involved in nuclear export of tumor suppressors such as p53...
  49. doi Structural determinants and mechanism of mammalian CRM1 allostery
    Nicole Dölker
    Abteilung für theoretische und computergestützte Biophysik, Max Planck Institut fur biophysikalische Chemie, Am Faßberg 11, 37077 Gottingen, Germany
    Structure 21:1350-60. 2013
    Proteins carrying nuclear export signals cooperatively assemble with the export factor CRM1 and the effector protein RanGTP...
  50. pmc The interaction of CRM1 and the nuclear pore protein Tpr
    Charles L Zhao
    Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California, Berkeley, California, United States of America
    PLoS ONE 9:e93709. 2014
    ..Recent work has demonstrated a binding interaction between the exportin CRM1 and the unstructured carboxylic tail of Tpr, on the nuclear basket...
  51. ncbi The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
    Cell 82:475-83. 1995
    ..BSA-R was itself exported from the nucleus in an active, saturable manner. Thus, the Rev activation domain constitutes a nuclear export signal that redirects RRE-containing viral RNAs to a non-mRNA export pathway...
  52. ncbi Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins
    H P Bogerd
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 82:485-94. 1995
    ..We conclude that Rab is the predicted activation domain-specific cofactor for the Rev/Rex class of RNA export factors...
  53. pmc The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88
    M Fornerod
    Department of Genetics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    EMBO J 16:807-16. 1997
    ..The 112 kDa protein is the human homologue of yeast CRM1, a protein known to be required for maintenance of correct chromosome structure...
  54. ncbi Evidence for a role of CRM1 in signal-mediated nuclear protein export
    B Ossareh-Nazari
    Institut Curie CNRS Unité Mixte de Recherche 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Science 278:141-4. 1997
    Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (..
  55. ncbi Exportin 1 (Crm1p) is an essential nuclear export factor
    K Stade
    Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0414, USA
    Cell 90:1041-50. 1997
    ..cerevisiae. The presented data are consistent with the conclusion that Crm1p is a carrier for the NES-mediated protein export pathway. We propose CRM1 be renamed exportin 1 (XPO1).
  56. ncbi Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins
    N Kudo
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku Tokyo 113
    J Biol Chem 272:29742-51. 1997
    b>Crm1 of Schizosaccharomyces pombe, a nuclear protein essential for proliferation and chromosome region maintenance, is a possible target of leptomycin B, an antifungal and antitumor antibiotic with cell cycle-arresting activity...
  57. ncbi The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export
    M Neville
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Curr Biol 7:767-75. 1997
    ..Crm1p has recently been shown to contribute to nuclear export in vertebrate systems. Here, we have studied this mechanism of nuclear to cytoplasmic transport...
  58. ncbi The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP
    P Askjaer
    Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 273:33414-22. 1998
    ..The cellular CRM1 (Exportin1) protein functions as a nuclear export receptor for proteins carrying a Rev-like NES in a process that ..
  59. pmc A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export
    R H Kehlenbach
    Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 145:645-57. 1999
    ..activated T cells) can be reconstituted in permeabilized cells with the GTPase Ran and the nuclear export receptor CRM1. We have now used this assay to identify another export factor...
  60. ncbi Putative reaction intermediates in Crm1-mediated nuclear protein export
    M Floer
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 274:16279-86. 1999
    We discovered several novel interactions between proteins involved in Crm1-mediated nuclear export of the nuclear export signal containing human immunodeficiency virus type 1 protein Rev...
  61. pmc Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region
    N Kudo
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 96:9112-7. 1999
    The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins...
  62. ncbi A novel nuclear export activity in HIV-1 matrix protein required for viral replication
    S Dupont
    Howard Hughes Medical Institute and Program in Molecular Medicine at the University of Massachusetts Medical Center, Worcester 01605, USA
    Nature 402:681-5. 1999
    ..We conclude that the MA nuclear export signal is required to counteract the MA nuclear localization signal, thus ensuring the cytoplasmic availability of the components required for virion assembly...
  63. ncbi PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation
    M Ohno
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 101:187-98. 2000
    ..Export depends upon the RNA cap structure, nuclear cap-binding complex (CBC), the export receptor CRM1/Xpo1, and RanGTP. These components are however insufficient to support U snRNA export...
  64. pmc Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export
    T Guan
    Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell Biol 20:5619-30. 2000
    ..Correspondingly, CRM1, the export receptor for leucine-rich export sequences, directly bound to a fragment of Nup50 in vitro, whereas ..
  65. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
    ..We found that the drug leptomycin B (LMB), which specifically inactivates the cellular CRM1 polypeptide, caused nuclear retention of NP in virus-infected cells, indicating a role for the CRM1 nuclear export ..
  66. pmc Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin
    W Hofmann
    Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
    J Cell Biol 152:895-910. 2001
    Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the export receptor CRM1/exportin1. However, additional protein factors interacting with leucine-rich NESs have been described...
  67. pmc Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export
    M E Lindsay
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22098, USA
    J Cell Biol 153:1391-402. 2001
    b>Crm1 is a member of the karyopherin family of nucleocytoplasmic transport receptors and mediates the export of proteins from the nucleus by forming a ternary complex with cargo and Ran:GTP...
  68. ncbi Two closely related human nuclear export factors utilize entirely distinct export pathways
    J Yang
    Howard Hughes Medical Institute, Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell 8:397-406. 2001
    ..NXF3 contains a novel Crm1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain...
  69. pmc RanBP3 influences interactions between CRM1 and its nuclear protein export substrates
    L Englmeier
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 2:926-32. 2001
    We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP...
  70. pmc Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAs
    Rui Yi
    Department of Microbiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 76:2036-42. 2002
    ..General agreement exists that Rev acts, at least in part, by recruiting the cellular Crm1 nuclear export factor to HIV-1 transcripts bearing the Rev response element RNA target, and thereby inducing their ..
  71. ncbi Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export
    Noriko Ishida
    Departments of Molecular and Cellular Biology and Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    J Biol Chem 277:14355-8. 2002
    ..Phosphorylation of p27(Kip1) on Ser(10) has now been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
  72. ncbi Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    Ran-binding protein 3 (RanBP3) is an approximately 55-kDa protein that functions as a cofactor for Crm1-mediated nuclear export. RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran.GTP and cargo...
  73. pmc The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions
    Yoshiyuki Hakata
    Institute for Genetic Medicine, Hokkaido University, Japan
    J Virol 76:8079-89. 2002
    ..the nuclear export of unspliced and singly spliced viral mRNAs by bridging viral RNA and export receptor human CRM1 (hCRM1)...
  74. pmc Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs
    Dusan Cmarko
    Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH 1005 Lausanne, Switzerland
    J Virol 76:10473-84. 2002
    ..The simultaneous demonstration of Rev, Tat, Br-labeled RNA, and cellular proteins SC35 and CRM1 in such fibrils reveals the potential of Rev to associate with nascent HIV pre-mRNA and its splicing complex and ..
  75. pmc Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindles
    Marshonna Forgues
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Bethesda, MD 20892, USA
    Mol Cell Biol 23:5282-92. 2003
    ..We recently showed that HBx induces cytoplasmic sequestration of Crm1, a nuclear export receptor that binds to Ran GTPase, thereby inducing nuclear localization of NF-kappaB...
  76. pmc A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein
    Yoshiyuki Hakata
    Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
    Mol Cell Biol 23:8751-61. 2003
    Human CRM1 (hCRM1) functions in the Rex-mediated mRNA export of human T-cell leukemia virus type 1 (HTLV-1) as an export receptor and as an inducing factor for Rex multimerization on its cognate RNA...
  77. pmc Somatic hypermutation is limited by CRM1-dependent nuclear export of activation-induced deaminase
    Kevin M McBride
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 199:1235-44. 2004
    ..Here we show that AID is actively excluded from the nucleus by an exportin CRM1-dependent pathway...
  78. pmc Automatic and quantitative measurement of protein-protein colocalization in live cells
    Sylvain V Costes
    Image Analysis Laboratory, National Cancer Institute, Frederick, Maryland, USA
    Biophys J 86:3993-4003. 2004
    ..further illustrated by measuring, for the first time in live cells, the dissociation rate (k(d)) of the HIV-1 Rev/CRM1 export complex induced by the cytotoxin leptomycin B...
  79. ncbi Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Cell 119:381-92. 2004
    ..Previously, CRM1 was identified as a cellular cofactor for Rev-dependent export of intron-containing HIV-1 RNA...
  80. pmc Kinetic and molecular analysis of nuclear export factor CRM1 association with its cargo in vivo
    Dirk Daelemans
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Mol Cell Biol 25:728-39. 2005
    The nucleocytoplasmic transport receptor CRM1 mediates the export of macromolecules from the nucleus to the cytoplasm by forming a ternary complex with a cargo molecule and RanGTP...
  81. ncbi Crm1 is a mitotic effector of Ran-GTP in somatic cells
    Alexei Arnaoutov
    Laboratory of Gene Regulation and Development, NICHD, NIH, Building 18, Room 106, Bethesda, MD 20892 5431, USA
    Nat Cell Biol 7:626-32. 2005
    ..Here we examine the mitotic function of Crm1, the Ran-GTP-binding nuclear export receptor for leucine-rich cargo (bearing nuclear export sequence) and ..
  82. ncbi Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication
    Wei Wang
    Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 7:823-30. 2005
    ..In addition to nucleocytoplasmic transport, the Ran-Crm1 network is involved in regulating centrosome duplication to ensure the formation of a bipolar spindle...
  83. ncbi Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl
    Oliver Hantschel
    Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 19 3, 1090 Vienna, Austria
    Mol Cell 19:461-73. 2005
    ..We propose that these interactions represent a major determinant for both Bcr-Abl and c-Abl localization...
  84. ncbi Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element
    Alexander T Prechtel
    Heinrich Pette Institute for Experimental Virology and Immunology, D 20251 Hamburg, Germany
    J Biol Chem 281:10912-25. 2006
    ..In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN ..
  85. ncbi CRM1, an RNA transporter, is a major species-specific restriction factor of human T cell leukemia virus type 1 (HTLV-1) in rat cells
    Xianfeng Zhang
    Institute for Genetic Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo 060 0815, Japan
    Microbes Infect 8:851-9. 2006
    Rat ortholog of human CRM1 has been found to be responsible for the poor activity of viral Rex protein, which is essential for RNA export of human T cell leukemia virus type 1 (HTLV-1)...
  86. ncbi Nuclear and cytoplasmic survivin: molecular mechanism, prognostic, and therapeutic potential
    Roland H Stauber
    Department of Otorhinolaryngology, Molecular and Cellular Oncology, University of Mainz, Mainz, Germany
    Cancer Res 67:5999-6002. 2007
    ..Recent evidence shows that the direct interaction of survivin with the nuclear export receptor Crm1 is critically involved in its intracellular localization and cancer-relevant functions...
  87. ncbi Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev
    Sergei Nekhai
    Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biolology, Howard University, NW Washington, DC 20059, USA
    Future Microbiol 1:417-26. 2006
    ..Rev primarily functions to export unspliced and partially spliced viral RNAs from the nucleus into the cytoplasm. For this activity, Rev cooperates with cellular transport protein CRM1 and RNA helicases DDX1 and DDX3, amongst others.
  88. doi Expression of the nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 is a prognostic factor in human ovarian cancer
    Aurelia Noske
    Institute of Pathology, Charite University Hospital, Berlin, Germany
    Cancer 112:1733-43. 2008
    The human nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) mediates the nuclear export of proteins and messenger RNAs and, thus, is an important regulator of subcellular distribution of key molecules...
  89. pmc Characterization of a CRM1-dependent nuclear export signal in the C terminus of herpes simplex virus type 1 tegument protein UL47
    Paul Williams
    Department of Virology, Faculty of Medicine, Imperial College London, London, United Kingdom
    J Virol 82:10946-52. 2008
    ..Finally, we show that the hUL47 NES is sensitive to the inhibitor of CRM1-mediated nuclear export leptomycin B...
  90. ncbi Cellular proteins and HIV-1 Rev function
    Modem Suhasini
    Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA
    Curr HIV Res 7:91-100. 2009
    ..While a number of reviews have focused on the host proteins (i.e., Crm1, importin-beta and nucleoporins) that specifically regulate shuttling of Rev between the nucleus and cytoplasm, we ..
  91. pmc Structural basis for assembly and disassembly of the CRM1 nuclear export complex
    Xiuhua Dong
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, USA
    Nat Struct Mol Biol 16:558-60. 2009
    CRM1 (or exportin 1, Xpo1) transports proteins out of the cell nucleus through the nuclear pore complex...
  92. pmc Sam68 functions in nuclear export and translation of HIV-1 RNA
    Johnny J He
    Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    RNA Biol 6:384-6. 2009
    ..Elucidation of the precise molecular function of Sam68 in HIV-1 gene expression is clearly warranted and is expected to unambiguously establish Sam68 as an important host factor for HIV-1 replication...
  93. ncbi Prognostic value of CRM1 in pancreas cancer
    Wei Yi Huang
    Department of Oncology, Affiliated People s 1st Hospital, Shanghai Jiaotong University, Shanghai, PR China 200085
    Clin Invest Med 32:E315. 2009
    ..The median survival is only 6 months. This study investigated the prognostic value of nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) expression in pancreas cancer.
  94. pmc Structural basis for cooperative RNA binding and export complex assembly by HIV Rev
    Matthew D Daugherty
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 17:1337-42. 2010
    ..the Rev response element (RRE) RNA, to form a highly specific oligomeric ribonucleoprotein (RNP) that binds to the Crm1 host export factor...
  95. doi NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1
    Thomas Guttler
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Nat Struct Mol Biol 17:1367-76. 2010
    Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs...
  96. doi Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells
    Mika Nagai-Fukataki
    Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
    Genes Cells 16:203-16. 2011
    ..the nuclear export of unspliced gag and singly spliced env mRNAs by bridging viral RNA and the export receptor, CRM1. Recently, rat CRM1 was found to be less efficient than human CRM1 in supporting Rev function in rats...
  97. pmc Rev-ing up post-transcriptional HIV-1 RNA expression
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, MD, USA
    RNA Biol 8:195-9. 2011
    ..Here, the post-transcriptional activities of Rev are discussed in the context of a recent finding that an RNA cap methyltransferase contributes to the expression of unspliced / partially spliced HIV-1 transcripts...
  98. pmc Insights into the function of the CRM1 cofactor RanBP3 from the structure of its Ran-binding domain
    Karla Langer
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS ONE 6:e17011. 2011
    Proteins bearing a leucine-rich nuclear export signal (NES) are exported from the nucleus by the transport factor CRM1, which forms a cooperative ternary complex with the NES-bearing cargo and with the small GTPase Ran...
  99. pmc The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway
    John H Coyle
    Myles H Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    RNA 17:1344-56. 2011
    ..introns, we used reporters in which the mRNA was exported either via the Nxf1/Nxt1 pathway using a CTE or via the Crm1 pathway using Rev/RRE...
  100. pmc Evolution of a species-specific determinant within human CRM1 that regulates the post-transcriptional phases of HIV-1 replication
    Nathan M Sherer
    Department of Infectious Diseases, King s College London School of Medicine, London, United Kingdom
    PLoS Pathog 7:e1002395. 2011
    ..virus type-1 (HIV-1) Rev protein regulates the nuclear export of intron-containing viral RNAs by recruiting the CRM1 nuclear export receptor...
  101. pmc Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domains
    Dirk Hoffmann
    Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS ONE 7:e38305. 2012
    ..Subsequently, Rev oligomerizes cooperatively and interacts with the cellular nuclear export receptor CRM1. In addition to mediating nuclear RNA export, Rev also affects the stability, translation and packaging of Rev-..

Research Grants53

  1. Cyclin D1 and mammary carcinoma
    J Diehl; Fiscal Year: 2009
    ..Finally, we propose to characterize newly identified cyclin D1 mutants for their ability to undergo active, CRM1-dependent nuclear export...
  2. Lizabeth A Allison; Fiscal Year: 2015
    ..TR1 follows a cooperative export pathway in which both calreticulin and the export factor CRM1 play a role in facilitating efficient translocation of the receptor from the nucleus to cytoplasm...
  3. Yuh Min Chook; Fiscal Year: 2016
    ..humans, only three classes of signals for Kap[unreadable]/Kap[unreadable]1, Kap[unreadable]2 (or Transportin) and CRM1, respectively, are known at this time...
  4. How Karyopherins Move Across The Nuclear Pore Complex
    Michael Rexach; Fiscal Year: 2006
    ..and iv) to conduct a comparative study (as delineated for Kap95p) for two additional karyopherins (the exportin Crm1 p, and the importin Kapi 04p) with the goal of uncovering general and specific features in the paths of karyopherin ..
  5. HTS Assay Development for Modulators of a Nuclear Import/Export Switch
    ISABELLA GRAEF; Fiscal Year: 2007
    ..between the NFATc family of transcription factors and the karyopherin ?2 (importin?, KPNA2) and exportin (Crm1, XPO1)...
  6. Screen for Small Molecule Inhibitors of Nuclear Protein Import
    Larry Gerace; Fiscal Year: 2007
    ..of small molecule inhibitors of the nuclear transport machinery has been described, which targets the exportin Crm1. The goal of this project is to develop and validate a high-throughput screening (HTS) assay involving ..
  7. Katherine L B Borden; Fiscal Year: 2016
    ..Our studies revealed that eIF4E dependent mRNA export is distinct from bulk mRNA export, being CRM1 dependent. We will examine the extent to which eIF4E dependent mRNA export uses the CRM1 export machinery...
  8. Nuclear export of the HIV genome: A Molecular Dynamics Study of the Interactions
    MOHAMMAD RK MOFRAD; Fiscal Year: 2013
    ..Nuclear export of the HIV genome: A Molecular Dynamics Study of the Interactions and Structure of HIV Rev and Host CRM1 during Export Summary The HIV virus is a pandemic lentivirus which has no existing cure or vaccine...
  9. MOLECULAR CHARACTERIZATION OF HERPES SIMPLEX VIRUS ICP27
    Stephen Rice; Fiscal Year: 2003
    ..We hypothesize that ICP27 interacts with and utilizes two distinct cellular export systems: CRM1 and REF/TAP...
  10. Structural requirements for the nuclear export of HIV RNA
    DAVID SCOTT BOOTH; Fiscal Year: 2013
    ..an intronic RNA structure called the Rev Response Element (RRE) and by recruiting the host nuclear export adaptors Crm1 and Ran...
  11. Zijie Sun; Fiscal Year: 2015
    ..Intriguingly, a functional HIV Rev-like leucine rich, CRM1/exportin regulated nuclear export signal (NES) was identified within the C-terminus of LZTS2...
  12. PATHWAYS OF NUCLEOCYTOPLASMIC TRANSPORT
    BRYCE PASCHAL; Fiscal Year: 2009
    ..by alanine mutations in AR phosphorylation sites, does not require new protein synthesis or the export receptor Crm1, and is reversible...
  13. Leptomycin B Development for Cancer Therapy
    Charles Hutchinson; Fiscal Year: 2004
    ..This microbial metabolite inhibits protein export from the cell nucleus by binding tightly to CRM1 (syn. exportin 1) and inhibiting its ability to shuttle protein complexes across the nuclear membrane...
  14. Identification of novel co-factors for HIV Tat and Rev as therapeutic targets
    Andrew P Rice; Fiscal Year: 2013
    ..The best understood cellular co-factor for Rev is termed XPO1 (CRM1);Rev binds directly to XPO1...
  15. Rev-dependent Nuclear Export of RNA
    Mirko Hennig; Fiscal Year: 2010
    ..interaction and the subsequent transport of intron-containing viral mRNA mediated by the cellular export receptor CRM1 are essential to the replication of the HIV-1 retrovirus...
  16. GUANYLATE CYCLASE TRANSDUCTION SYSTEMS IN RETINA
    Rameshwar Sharma; Fiscal Year: 2002
    ..involved in GCAPs and S100beta stimulation of ROS-GC have been localized and termed Ca/2+-regulated modules (CRMs): CRM1 is linked to GCAP1 signaling. CRM2 to S100beta and CRM3 to GCAP2...
  17. REGULATION OF C-REL BY IKB-ALPH
    Mark Hannink; Fiscal Year: 2003
    ..The function of IkappaBalpha as an adaptor protein that bridges c-Rel with the export receptor, Crm1, will be examined...
  18. Leptomycin B Analogs
    YOUNG LI; Fiscal Year: 2005
    ..Many cell signaling processes, including ones essential to the specific onset and development of cancer, rely on CRM1 mediated nuclear export...
  19. Inhibition of colon tumor progression by Ink4a/Arf
    Gregory Enders; Fiscal Year: 2007
    ....
  20. Formation of Heterochromatin and Cellular Senescence Driven by HIRA and ASF 1a
    Gregory Enders; Fiscal Year: 2009
    ..Specific Aim 3. Investigate the role of GSK3 activity in localization of HIRA to PML bodies, formation of SAHF and onset of senescence. ..
  21. G2 Role For Cdk2 in Human Cells
    Gregory Enders; Fiscal Year: 2007
    ..We believe that deregulation of Cdk2, a frequent event in cancer, may contribute to genetic instability and neoplastic progression through deregulation of these functions. ..
  22. Potent inhibitors of Anthrax Lethal Factor
    Peter Vogt; Fiscal Year: 2003
    ..Libraries will be targeted using the structural information available for LF, supplemented by information from point mutations of the substrate. Information from our previous synthesis of metalloprotease inhibitors will also be used. ..
  23. Effect of APOBEC3G on retroviruses and retrotransposons
    BRYAN CULLEN; Fiscal Year: 2009
    ..abstract_text> ..
  24. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  25. Nuclear Import Pathways for HPV Macromolecules
    JUNONA F MOROIANU; Fiscal Year: 2010
    ..Overall, the studies proposed in this renewal application will continue to advance our understanding of major nuclear import pathways relevant to the papillomavirus infection and pathogenesis. ..
  26. NFKB IN INHIBITION OF CHEMOTHERAPY INDUCED APOPTOSIS
    James Cusack; Fiscal Year: 2004
    ..abstract_text> ..
  27. Translational Control of Retroviral Unspliced mRNA
    KATHLEEN A BORIS LAWRIE; Fiscal Year: 2010
    ..abstract_text> ..
  28. Drug Abuse and NeuroAIDS in China
    Johnny He; Fiscal Year: 2008
    ..The social and economic impact can not be overemphasized. The current study seeks to have a better understanding of the prevalence and nature of these diseases in the selected population. [unreadable] [unreadable] [unreadable]..
  29. THE ONCOGENICITY OF WINGED HELIX PROTEINS
    Peter Vogt; Fiscal Year: 2003
    ..The nature of activating mutations, the interactions with the preinitiation complex, and the identity and function of target genes will reveal novel molecular targets for therapeutic intervention. ..
  30. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2003
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
  31. Quasispecies Evolution During Lentivirus Persistence
    Susan Carpenter; Fiscal Year: 2003
    ..abstract_text> ..
  32. DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1
    Mark Wainberg; Fiscal Year: 2001
    ..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
  33. HIV-1 TROPISM AND CHEMOKINE RECEPTORS
    BRYAN CULLEN; Fiscal Year: 2002
    ..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
  34. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  35. The Role of NF-kappaB in Chemotherapy Resistance
    James Cusack; Fiscal Year: 2009
    ..This proposal has the potential to completely alter chemotherapy strategies based on our understanding of the role of NF-kappaB in chemotherapy resistance. ..
  36. Regulation of histone deacetylase 7 (HDAC7) activity
    Hung Ying Kao; Fiscal Year: 2006
    ..Understanding how cellular signaling controls HDAC activities will help to understand deregulated cell growth in cancer and may have therapeutic implications for cancer treatment. ..
  37. TARGET-GUIDED DEVELOPMENT OF SPECIFIC AKT INHIBITORS
    Peter Vogt; Fiscal Year: 2008
    ..4) Investigate the structure-activity relationship through in situ click chemistry and optimize inhibitors for potency, selectivity and cell-based activity ..
  38. Controlling the Max Network
    Peter Vogt; Fiscal Year: 2008
    ..The proposed work on stabilizers in the Myc-Max network could open the door to a new way of influencing and directing cell growth. ..
  39. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  40. Nuclear functions of the tumor suppressor protein APC
    KRISTI NEUFELD; Fiscal Year: 2008
    ..Greater knowledge of APC function in normal cells will improve our understanding of APC's role in tumorigenesis and ultimately illuminate new points for therapeutic intervention. ..
  41. MECHANISMS OF HIV-1 ENTRY AND PATHOGENESIS IN ASTROCYTES
    Johnny He; Fiscal Year: 2008
    ..They should also aid in the development of strategies for treating neurologic symptoms of AIDS patients. ..
  42. Combinatorial Chemistry and Cancer Target
    Peter Vogt; Fiscal Year: 2008
    ..The collaborating groups of this Program Project have become an integrated unit that incorporates a wide range of expertise and interests and applies them to the common goal, new anticancer compounds. ..
  43. Use a novel Tat transgenic model to develop neuroAIDS therapeutics
    Johnny He; Fiscal Year: 2007
    ..The proposed study is to explore the possibility of using a mouse model to develop, evaluate, and validate therapeutic strategies specifically targeted at these diseases. [unreadable] [unreadable] [unreadable]..
  44. Regulation of human microRNA biosynthesis and activity
    BRYAN CULLEN; Fiscal Year: 2007
    ..Finally, in specific aim 5, we seek to extend our exciting initial data demonstrating inhibition of miRNA function by the adenovirus VA1 non-coding RNA by trying to define the underlying mechanism. ..
  45. c-Kit Mutations and Their Role in Tumor Biology
    Cheryl London; Fiscal Year: 2005
    ..abstract_text> ..
  46. Molecular Mechanism of HIV-1 Vif Function
    BRYAN CULLEN; Fiscal Year: 2007
    ..Together, this wide-ranging series of experiments should lead to considerable insight into how HIV-1 Vif functions and potentially suggest approaches to inhibit Vif function. ..
  47. HEPATIC CARCINOGENESIS: MOLECULAR MECHANISMS
    Martina Buck; Fiscal Year: 2006
    ..abstract_text> ..
  48. Epo-dependent JAK2 Signaling in Painful Neuropathy
    WENDY CAMPANA; Fiscal Year: 2006
    ..The experiments will provide new information on the basic mechanisms of nerve injury and pain, and there from, new rationale for development of novel neuroprotective strategies for preventing chronic neuropathic pain. ..
  49. Tat Interaction with LRP and Its Role in AIDS Dementia
    Johnny He; Fiscal Year: 2006
    ..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..