Genomes and Genes
Gene Symbol: XPO1
Description: exportin 1
Alias: CRM1, emb, exp1, exportin-1, chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, exportin 1 (CRM1 homolog, yeast), exportin-1 (required for chromosome region maintenance)
Publications234 found, 100 shown here
- Transcriptional and posttranscriptional regulation of HIV-1 gene expressionJonathan Karn
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
Cold Spring Harb Perspect Med 2:a006916. 2012..In cells that are not fully activated, limiting levels of Tat and Rev act as potent blocks to premature virus production...
- Inhibition of CRM1-mediated nucleocytoplasmic transport: triggering human melanoma cell apoptosis by perturbing multiple cellular pathwaysGaurav Pathria
Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria
J Invest Dermatol 132:2780-90. 2012..DNA microarray analysis, we identified four key components of the nucleocytoplasmic transport machinery-CRM1, RAN (RAN-GTPase), RANGAP1, and RANBP1-to be overexpressed in human melanoma metastases...
- A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit exportThomas Wild
Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
PLoS Biol 8:e1000522. 2010..We show that Exp5, like the known 60S exportin Crm1, binds to pre-60S particles in a RanGTP-dependent manner...
- Cessation of HIV-1 transcription by inhibiting regulatory protein Rev-mediated RNA transportYuan Cao
Institute of Medicinal Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan, P R China
Curr HIV Res 7:101-8. 2009..and cytoplasm, facilitates export of unspliced and singly spliced viral transcripts containing RRE RNA by the CRM1 export pathway...
- Importin 7 and exportin 1 link c-Myc and p53 to regulation of ribosomal biogenesisLior Golomb
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Mol Cell 45:222-32. 2012..We report that transcription of the β-karyopherin genes importin 7 (IPO7) and exportin 1 (XPO1), and several additional nuclear import receptors, is regulated positively by c-Myc and negatively by p53...
- The structural biology of HIV-1: mechanistic and therapeutic insightsAlan Engelman
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
Nat Rev Microbiol 10:279-90. 2012..Here, we review recent advances in HIV-1 structural biology, focusing on the molecular mechanisms of viral replication and on the development of new therapeutics...
- The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substratesA Bachi
European Molecular Biology Laboratory, Heidelberg, Germany
RNA 6:136-58. 2000....
- Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactorsA Kiss
Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
Virology 314:591-600. 2003..These results suggest a physiological role for hNup98 in modulating Rev-dependent RNA export during HIV infection...
- Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7Yoshitaka Tajima
Department of Biochemistry, The Cancer Institute of the Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170 8455, Japan
J Biol Chem 278:10716-21. 2003..In transfected cells, the Smurf1-Smad7 complex was accumulated in the cytoplasm by the nuclear export receptor, CRM1; this action was prevented by treatment with leptomycin B, a specific inactivator of CRM1 function...
- Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteinsSupratik Das
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
Methods Mol Biol 485:271-93. 2009..Once the mutants are isolated, they are tested in other biochemical systems and can be subjected to further functional and virological studies...
- Identification of poly (ADP-ribose) polymerase-1 (PARP-1) as a novel Kruppel-like factor 8-interacting and -regulating proteinHeng Lu
Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida 32827, USA
J Biol Chem 286:20335-44. 2011..This mislocalization was prevented by either PARP-1 re-expression or inhibition of CRM1-dependent nuclear export. Interestingly, co-IP indicated competition between PARP-1 and CRM1 for KLF8 binding...
- Nuclear-to-cytoplasmic relocalization of the proliferating cell nuclear antigen (PCNA) during differentiation involves a chromosome region maintenance 1 (CRM1)-dependent export and is a prerequisite for PCNA antiapoptotic activity in mature neutrophilsDikra Bouayad
Inserm U1016, 75014 Paris, France
J Biol Chem 287:33812-25. 2012..We showed here that leptomycin B, an inhibitor of the chromosome region maintenance 1 (CRM1) exportin, inhibited PCNA relocalization during granulocytic differentiation of HL-60 and NB4 promyelocytic cell ..
- Active nuclear import and export pathways regulate E2F-5 subcellular localizationMargarita D Apostolova
Department of Physiology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 277:34471-9. 2002..Indeed, E2F-5 is exported from the nucleus through leptomycin B-sensitive, CRM1-mediated transport, through a region corresponding to amino acid residues 130-154...
- Epstein-Barr virus LMP1 blocks p16INK4a-RB pathway by promoting nuclear export of E2F4/5Naoko Ohtani
Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, UK
J Cell Biol 162:173-83. 2003..Here, we report that LMP1 promotes the CRM1-dependent nuclear export of Ets2, which is an important transcription factor for p16INK4a gene expression, thereby ..
- A CRM1-dependent nuclear export pathway is involved in the regulation of IRF-5 subcellular localizationRongtuan Lin
Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, McGill University, Montreal, Quebec H3T 1E2, Canada
J Biol Chem 280:3088-95. 2005..In the present study, we demonstrate that a CRM1-dependent nuclear export pathway is involved in the regulation of IRF-5 subcellular localization...
- Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutantsA M Szilvay
National Centre for Research in Virology, Gade Institute, University of Bergen, Norway
J Virol 69:3315-23. 1995..In cotransfection experiments, mutants containing mutations of Rev inhibited the nuclear egress of wild-type Rev, thus revealing a novel transdominant negative phenotype...
- Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1A S Zolotukhin
Human Retrovirus Pathogenesis Group, NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
J Biol Chem 272:11356-60. 1997..These data suggest that RanBP1 interacts with Rev at the putative nuclear receptor and, hence, shares a step in posttranscriptional pathway with Rev...
- A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1G Farjot
Laboratoire de Virologie, ENS Lyon, Unité INSERM U 412, 46, Allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 274:17309-17. 1999..We show here that NLP-1, like the previously described Rev-interacting protein hRIP/Rab and several nucleoporins, also interacts with CRM-1 both in yeast and mammalian cells...
- The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1B B Singh
Department of Pharmacology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 274:37370-8. 1999..Here, we show the zinc fingers of RanBP2 associate with high specificity to the nuclear export factor, exportin-1 (CRM1)...
- Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor functionX H Liang
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Cancer Res 61:3443-9. 2001..signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport...
- Alternative splicing of Staufen2 creates the nuclear export signal for CRM1 (Exportin 1)Takashi Miki
Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Japan
J Biol Chem 279:47473-9. 2004..from the nucleus by two distinct pathways, one of which is RBD3-mediated and the other of which is CRM1 (exportin 1)-dependent...
- Camptothecin induces nuclear export of prohibitin preferentially in transformed cells through a CRM-1-dependent mechanismShipra Rastogi
Drug Discovery Program, Department of Interdisciplinary Oncology, H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
J Biol Chem 281:2951-9. 2006..These results suggest that the regulated translocation of prohibitin from the nucleus to the mitochondria facilitates its pleiotropic functions and might contribute to its anti-proliferative and tumor suppressive properties...
- Differential IFN-gamma stimulation of HLA-A gene expression through CRM-1-dependent nuclear RNA exportSarah K Browne
Massey Cancer Center, Virginia Commonwealth University Medical Center, 401 College Street, Richmond, VA 23298, USA
J Immunol 177:8612-9. 2006....
- Modulating HIV-1 RNA processing and utilizationMeredith McLaren
Department of Molecular Genetics, University of Toronto, Toronto, Ontario
Front Biosci 13:5693-707. 2008..The observations made underline the multiple fate decisions that must be made at each stage of the viral RNA metabolic pathway and highlight potential new avenues for controlling HIV-1 replication...
- Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
Virus Genes 40:341-6. 2010..Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death.
- Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-α 1 mRNATominori Kimura
Laboratory of Microbiology and Cell Biology, Department of Pharmacy, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga 525 8577, Japan
Med Mol Morphol 43:145-57. 2010We recently reported the chromosome region maintenance 1 (CRM1)-dependent nuclear export of intron-less human interferon-α1 (IFN-α1) mRNA, which encodes a main effecter of host innate immunity...
- Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1O Rosorius
Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Schlossgarten 4, D 91054 Erlangen, Germany
J Cell Sci 112:2369-80. 1999..We are able to show that eIF-5A interacts with the general nuclear export receptor, CRM1. Furthermore, microinjection studies in somatic cells revealed that eIF-5A is transported from the nucleus to the ..
- Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1K Plafker
Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
Mol Cell Biol 20:3510-21. 2000..Shuttling of RanBP1 may function to clear nuclear pores of Ran:GTP, to prevent premature release of import cargo from transport receptors...
- Nuclear targeting of adenovirus type 2 requires CRM1-mediated nuclear exportSten Strunze
University of Zurich, Institute of Zoology, CH 8057 Zurich, Switzerland
Mol Biol Cell 16:2999-3009. 2005..Two distinct inhibitors of the nuclear export factor CRM1, leptomycin B (LMB) and ratjadone A (RJA) or CRM1-siRNAs blocked adenovirus infection, arrested cytoplasmic ..
- Endosomal transport of ErbB-2: mechanism for nuclear entry of the cell surface receptorDipak K Giri
Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, 77030, USA
Mol Cell Biol 25:11005-18. 2005..This pathway may serve as a general mechanism to allow direct communication between cell surface receptors and the nucleus, and our findings thus open a new era in understanding direct trafficking between the cell membrane and nucleus...
- Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transportF R Bischoff
Division of Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
Proc Natl Acad Sci U S A 92:1749-53. 1995..Thus, a defect in either of the two antagonistic regulators of Ran would result in a shutdown of Ran-dependent transport processes, in agreement with the almost identical phenotypes described for such defects in budding yeast...
- CRM1 is an export receptor for leucine-rich nuclear export signalsM Fornerod
European Molecular Biology Laboratory, Heidelberg, Germany
Cell 90:1051-60. 1997b>CRM1 is distantly related to receptors that mediate nuclear protein import and was previously shown to interact with the nuclear pore complex. Overexpression of CRM1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus...
- CRM1-mediated recycling of snurportin 1 to the cytoplasmE Paraskeva
Zentrum fur Molekulare Biologie der Universitat Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 145:255-64. 1999..We have shown previously that CAS mediates export of importin alpha. Here we show that snurportin 1 is exported by CRM1, the receptor for leucine-rich nuclear export signals (NESs)...
- An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev proteinJ Yang
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 96:13404-8. 1999..Like HIV-1 Rev, K-Rev binds to both the Crm1 nuclear export factor and to a cis-acting viral RNA target to activate nuclear export of unspliced RNAs...
- Transport between the cell nucleus and the cytoplasmD Gorlich
Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany
Annu Rev Cell Dev Biol 15:607-60. 1999..We discuss mechanistic aspects and the energetics of transport receptor function and describe a number of pathways in detail...
- Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathwayFranziska Thomas
Swiss Federal Institute of Technology ETH Zurich, Institute of Biochemistry, HPM F11 1, Switzerland
J Cell Sci 116:2409-19. 2003..Most nuclear export pathways require RanGTP-binding export receptors. We analyzed the role of CRM1, the export receptor for leucine-rich nuclear export signals (NES), in the biogenesis of ribosomal subunits in ..
- Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebratesChristopher R Trotta
Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA
EMBO J 22:2841-51. 2003..We show that in vertebrate cells, transport of both subunits requires the export receptor CRM1 and Ran.GTP. Export of 60S subunits is coupled with that of the nucleo- cytoplasmic shuttling protein NMD3...
- Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complexCarlo Petosa
European Molecular Biology Laboratory, Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
Mol Cell 16:761-75. 2004b>CRM1/Exportin1 mediates the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran...
- The expression of CRM1 is associated with prognosis in human osteosarcomaYang Yao
Department of Oncology, Affiliated 6th People s Hospital, Shanghai Jiaotong University, Shanghai 200223, P R China
Oncol Rep 21:229-35. 2009The nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) is involved in the nuclear export of proteins and messenger RNAs and, thus, mediates the subcellular distribution of important molecules...
- The Karyopherin proteins, Crm1 and Karyopherin beta1, are overexpressed in cervical cancer and are critical for cancer cell survival and proliferationPauline J van der Watt
Division of Medical Biochemistry, Faculty of Health Sciences, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa
Int J Cancer 124:1829-40. 2009..In this study, we examined the expression of the Karyopherins, Crm1, Karyopherin beta1 (Kpnbeta1) and Karyopherin alpha2 (Kpnalpha2), in cervical tissue and cell lines...
- Structural basis for leucine-rich nuclear export signal recognition by CRM1Xiuhua Dong
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park, Dallas, Texas 75390 9041, USA
Nature 458:1136-41. 2009CRM1 (also known as XPO1 and exportin 1) mediates nuclear export of hundreds of proteins through the recognition of the leucine-rich nuclear export signal (LR-NES). Here we present the 2.9 A structure of CRM1 bound to snurportin 1 (SNUPN)...
- Active nuclear import and cytoplasmic retention of activation-induced deaminaseAnne Marie Patenaude
Institut de Recherches Cliniques de Montreal, Quebec, Canada
Nat Struct Mol Biol 16:517-27. 2009..Blocking nuclear import alters the balance between these processes in favor of cytoplasmic retention, resulting in reduced isotype class switching...
- Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophagesHiroyuki Okada
Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
Retrovirology 6:43. 2009..basis for developing a better rat model for HIV-1 infection, we evaluated the effect of human CyclinT1 (hCycT1) and CRM1 (hCRM1) on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells ..
- Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPaseTominori Kimura
Laboratory of Microbiology and Cell Biology, Department of Pharmacy, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga, 525 8577, Japan
Med Mol Morphol 42:70-81. 2009..The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export...
- Expression of CRM1 in human gliomas and its significance in p27 expression and clinical prognosisAiguo Shen
The Jiangsu Province Key Lab of Neuroregeneration, Nantong University, Nantong, China
Neurosurgery 65:153-9; discussion 159-60. 2009..It is believed that phosphorylation of p27 on Ser10 has been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
- An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1Masako Koyama
Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya, Japan
EMBO J 29:2002-13. 2010The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES)...
- Electrostatic interactions involving the extreme C terminus of nuclear export factor CRM1 modulate its affinity for cargoAbigail M Fox
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
J Biol Chem 286:29325-35. 2011The toroid-shaped nuclear protein export factor CRM1 is constructed from 21 tandem HEAT repeats, each of which contains an inner (B) and outer (A) α-helix joined by loops...
- Prognostic impact and targeting of CRM1 in acute myeloid leukemiaKensuke Kojima
Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
Blood 121:4166-74. 2013Chromosomal region maintenance 1 (CRM1) is a nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal. CRM1 is involved in nuclear export of tumor suppressors such as p53...
- Structural determinants and mechanism of mammalian CRM1 allosteryNicole Dölker
Abteilung für theoretische und computergestützte Biophysik, Max Planck Institut fur biophysikalische Chemie, Am Faßberg 11, 37077 Gottingen, Germany
Structure 21:1350-60. 2013Proteins carrying nuclear export signals cooperatively assemble with the export factor CRM1 and the effector protein RanGTP...
- The interaction of CRM1 and the nuclear pore protein TprCharles L Zhao
Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California, Berkeley, California, United States of America
PLoS ONE 9:e93709. 2014..Recent work has demonstrated a binding interaction between the exportin CRM1 and the unstructured carboxylic tail of Tpr, on the nuclear basket...
- The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAsU Fischer
Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
Cell 82:475-83. 1995..BSA-R was itself exported from the nucleus in an active, saturable manner. Thus, the Rev activation domain constitutes a nuclear export signal that redirects RRE-containing viral RNAs to a non-mRNA export pathway...
- Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteinsH P Bogerd
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
Cell 82:485-94. 1995..We conclude that Rab is the predicted activation domain-specific cofactor for the Rev/Rex class of RNA export factors...
- The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88M Fornerod
Department of Genetics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
EMBO J 16:807-16. 1997..The 112 kDa protein is the human homologue of yeast CRM1, a protein known to be required for maintenance of correct chromosome structure...
- Evidence for a role of CRM1 in signal-mediated nuclear protein exportB Ossareh-Nazari
Institut Curie CNRS Unité Mixte de Recherche 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Science 278:141-4. 1997Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (..
- Exportin 1 (Crm1p) is an essential nuclear export factorK Stade
Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0414, USA
Cell 90:1041-50. 1997..cerevisiae. The presented data are consistent with the conclusion that Crm1p is a carrier for the NES-mediated protein export pathway. We propose CRM1 be renamed exportin 1 (XPO1).
- Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteinsN Kudo
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku Tokyo 113
J Biol Chem 272:29742-51. 1997b>Crm1 of Schizosaccharomyces pombe, a nuclear protein essential for proliferation and chromosome region maintenance, is a possible target of leptomycin B, an antifungal and antitumor antibiotic with cell cycle-arresting activity...
- The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear exportM Neville
Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
Curr Biol 7:767-75. 1997..Crm1p has recently been shown to contribute to nuclear export in vertebrate systems. Here, we have studied this mechanism of nuclear to cytoplasmic transport...
- The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTPP Askjaer
Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
J Biol Chem 273:33414-22. 1998..The cellular CRM1 (Exportin1) protein functions as a nuclear export receptor for proteins carrying a Rev-like NES in a process that ..
- A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear exportR H Kehlenbach
Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Cell Biol 145:645-57. 1999..activated T cells) can be reconstituted in permeabilized cells with the GTPase Ran and the nuclear export receptor CRM1. We have now used this assay to identify another export factor...
- Putative reaction intermediates in Crm1-mediated nuclear protein exportM Floer
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 274:16279-86. 1999We discovered several novel interactions between proteins involved in Crm1-mediated nuclear export of the nuclear export signal containing human immunodeficiency virus type 1 protein Rev...
- Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved regionN Kudo
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
Proc Natl Acad Sci U S A 96:9112-7. 1999The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins...
- A novel nuclear export activity in HIV-1 matrix protein required for viral replicationS Dupont
Howard Hughes Medical Institute and Program in Molecular Medicine at the University of Massachusetts Medical Center, Worcester 01605, USA
Nature 402:681-5. 1999..We conclude that the MA nuclear export signal is required to counteract the MA nuclear localization signal, thus ensuring the cytoplasmic availability of the components required for virion assembly...
- PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylationM Ohno
European Molecular Biology Laboratory, Heidelberg, Germany
Cell 101:187-98. 2000..Export depends upon the RNA cap structure, nuclear cap-binding complex (CBC), the export receptor CRM1/Xpo1, and RanGTP. These components are however insufficient to support U snRNA export...
- Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein exportT Guan
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Cell Biol 20:5619-30. 2000..Correspondingly, CRM1, the export receptor for leucine-rich export sequences, directly bound to a fragment of Nup50 in vitro, whereas ..
- Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathwayD Elton
Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
J Virol 75:408-19. 2001..We found that the drug leptomycin B (LMB), which specifically inactivates the cellular CRM1 polypeptide, caused nuclear retention of NP in virus-infected cells, indicating a role for the CRM1 nuclear export ..
- Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actinW Hofmann
Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
J Cell Biol 152:895-910. 2001Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the export receptor CRM1/exportin1. However, additional protein factors interacting with leucine-rich NESs have been described...
- Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein exportM E Lindsay
Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22098, USA
J Cell Biol 153:1391-402. 2001b>Crm1 is a member of the karyopherin family of nucleocytoplasmic transport receptors and mediates the export of proteins from the nucleus by forming a ternary complex with cargo and Ran:GTP...
- Two closely related human nuclear export factors utilize entirely distinct export pathwaysJ Yang
Howard Hughes Medical Institute, Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
Mol Cell 8:397-406. 2001..NXF3 contains a novel Crm1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain...
- RanBP3 influences interactions between CRM1 and its nuclear protein export substratesL Englmeier
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
EMBO Rep 2:926-32. 2001We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP...
- Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAsRui Yi
Department of Microbiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 76:2036-42. 2002..General agreement exists that Rev acts, at least in part, by recruiting the cellular Crm1 nuclear export factor to HIV-1 transcripts bearing the Rev response element RNA target, and thereby inducing their ..
- Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear exportNoriko Ishida
Departments of Molecular and Cellular Biology and Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
J Biol Chem 277:14355-8. 2002..Phosphorylation of p27(Kip1) on Ser(10) has now been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
- Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factorMichael E Nemergut
Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 277:17385-8. 2002Ran-binding protein 3 (RanBP3) is an approximately 55-kDa protein that functions as a cofactor for Crm1-mediated nuclear export. RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran.GTP and cargo...
- The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functionsYoshiyuki Hakata
Institute for Genetic Medicine, Hokkaido University, Japan
J Virol 76:8079-89. 2002..the nuclear export of unspliced and singly spliced viral mRNAs by bridging viral RNA and export receptor human CRM1 (hCRM1)...
- Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAsDusan Cmarko
Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH 1005 Lausanne, Switzerland
J Virol 76:10473-84. 2002..The simultaneous demonstration of Rev, Tat, Br-labeled RNA, and cellular proteins SC35 and CRM1 in such fibrils reveals the potential of Rev to associate with nascent HIV pre-mRNA and its splicing complex and ..
- Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindlesMarshonna Forgues
Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Bethesda, MD 20892, USA
Mol Cell Biol 23:5282-92. 2003..We recently showed that HBx induces cytoplasmic sequestration of Crm1, a nuclear export receptor that binds to Ran GTPase, thereby inducing nuclear localization of NF-kappaB...
- A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex proteinYoshiyuki Hakata
Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
Mol Cell Biol 23:8751-61. 2003Human CRM1 (hCRM1) functions in the Rex-mediated mRNA export of human T-cell leukemia virus type 1 (HTLV-1) as an export receptor and as an inducing factor for Rex multimerization on its cognate RNA...
- Somatic hypermutation is limited by CRM1-dependent nuclear export of activation-induced deaminaseKevin M McBride
Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
J Exp Med 199:1235-44. 2004..Here we show that AID is actively excluded from the nucleus by an exportin CRM1-dependent pathway...
- Automatic and quantitative measurement of protein-protein colocalization in live cellsSylvain V Costes
Image Analysis Laboratory, National Cancer Institute, Frederick, Maryland, USA
Biophys J 86:3993-4003. 2004..further illustrated by measuring, for the first time in live cells, the dissociation rate (k(d)) of the HIV-1 Rev/CRM1 export complex induced by the cytotoxin leptomycin B...
- Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export functionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
Cell 119:381-92. 2004..Previously, CRM1 was identified as a cellular cofactor for Rev-dependent export of intron-containing HIV-1 RNA...
- Kinetic and molecular analysis of nuclear export factor CRM1 association with its cargo in vivoDirk Daelemans
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
Mol Cell Biol 25:728-39. 2005The nucleocytoplasmic transport receptor CRM1 mediates the export of macromolecules from the nucleus to the cytoplasm by forming a ternary complex with a cargo molecule and RanGTP...
- Crm1 is a mitotic effector of Ran-GTP in somatic cellsAlexei Arnaoutov
Laboratory of Gene Regulation and Development, NICHD, NIH, Building 18, Room 106, Bethesda, MD 20892 5431, USA
Nat Cell Biol 7:626-32. 2005..Here we examine the mitotic function of Crm1, the Ran-GTP-binding nuclear export receptor for leucine-rich cargo (bearing nuclear export sequence) and ..
- Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplicationWei Wang
Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, Bethesda, MD 20892, USA
Nat Cell Biol 7:823-30. 2005..In addition to nucleocytoplasmic transport, the Ran-Crm1 network is involved in regulating centrosome duplication to ensure the formation of a bipolar spindle...
- Structural basis for the cytoskeletal association of Bcr-Abl/c-AblOliver Hantschel
Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 19 3, 1090 Vienna, Austria
Mol Cell 19:461-73. 2005..We propose that these interactions represent a major determinant for both Bcr-Abl and c-Abl localization...
- Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA elementAlexander T Prechtel
Heinrich Pette Institute for Experimental Virology and Immunology, D 20251 Hamburg, Germany
J Biol Chem 281:10912-25. 2006..In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN ..
- CRM1, an RNA transporter, is a major species-specific restriction factor of human T cell leukemia virus type 1 (HTLV-1) in rat cellsXianfeng Zhang
Institute for Genetic Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo 060 0815, Japan
Microbes Infect 8:851-9. 2006Rat ortholog of human CRM1 has been found to be responsible for the poor activity of viral Rex protein, which is essential for RNA export of human T cell leukemia virus type 1 (HTLV-1)...
- Nuclear and cytoplasmic survivin: molecular mechanism, prognostic, and therapeutic potentialRoland H Stauber
Department of Otorhinolaryngology, Molecular and Cellular Oncology, University of Mainz, Mainz, Germany
Cancer Res 67:5999-6002. 2007..Recent evidence shows that the direct interaction of survivin with the nuclear export receptor Crm1 is critically involved in its intracellular localization and cancer-relevant functions...
- Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and RevSergei Nekhai
Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biolology, Howard University, NW Washington, DC 20059, USA
Future Microbiol 1:417-26. 2006..Rev primarily functions to export unspliced and partially spliced viral RNAs from the nucleus into the cytoplasm. For this activity, Rev cooperates with cellular transport protein CRM1 and RNA helicases DDX1 and DDX3, amongst others.
- Expression of the nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 is a prognostic factor in human ovarian cancerAurelia Noske
Institute of Pathology, Charite University Hospital, Berlin, Germany
Cancer 112:1733-43. 2008The human nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) mediates the nuclear export of proteins and messenger RNAs and, thus, is an important regulator of subcellular distribution of key molecules...
- Characterization of a CRM1-dependent nuclear export signal in the C terminus of herpes simplex virus type 1 tegument protein UL47Paul Williams
Department of Virology, Faculty of Medicine, Imperial College London, London, United Kingdom
J Virol 82:10946-52. 2008..Finally, we show that the hUL47 NES is sensitive to the inhibitor of CRM1-mediated nuclear export leptomycin B...
- Cellular proteins and HIV-1 Rev functionModem Suhasini
Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA
Curr HIV Res 7:91-100. 2009..While a number of reviews have focused on the host proteins (i.e., Crm1, importin-beta and nucleoporins) that specifically regulate shuttling of Rev between the nucleus and cytoplasm, we ..
- Structural basis for assembly and disassembly of the CRM1 nuclear export complexXiuhua Dong
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, USA
Nat Struct Mol Biol 16:558-60. 2009CRM1 (or exportin 1, Xpo1) transports proteins out of the cell nucleus through the nuclear pore complex...
- Sam68 functions in nuclear export and translation of HIV-1 RNAJohnny J He
Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
RNA Biol 6:384-6. 2009..Elucidation of the precise molecular function of Sam68 in HIV-1 gene expression is clearly warranted and is expected to unambiguously establish Sam68 as an important host factor for HIV-1 replication...
- Prognostic value of CRM1 in pancreas cancerWei Yi Huang
Department of Oncology, Affiliated People s 1st Hospital, Shanghai Jiaotong University, Shanghai, PR China 200085
Clin Invest Med 32:E315. 2009..The median survival is only 6 months. This study investigated the prognostic value of nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) expression in pancreas cancer.
- Structural basis for cooperative RNA binding and export complex assembly by HIV RevMatthew D Daugherty
Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, California, USA
Nat Struct Mol Biol 17:1337-42. 2010..the Rev response element (RRE) RNA, to form a highly specific oligomeric ribonucleoprotein (RNP) that binds to the Crm1 host export factor...
- NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1Thomas Guttler
Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
Nat Struct Mol Biol 17:1367-76. 2010Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs...
- Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cellsMika Nagai-Fukataki
Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
Genes Cells 16:203-16. 2011..the nuclear export of unspliced gag and singly spliced env mRNAs by bridging viral RNA and the export receptor, CRM1. Recently, rat CRM1 was found to be less efficient than human CRM1 in supporting Rev function in rats...
- Rev-ing up post-transcriptional HIV-1 RNA expressionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, MD, USA
RNA Biol 8:195-9. 2011..Here, the post-transcriptional activities of Rev are discussed in the context of a recent finding that an RNA cap methyltransferase contributes to the expression of unspliced / partially spliced HIV-1 transcripts...
- Insights into the function of the CRM1 cofactor RanBP3 from the structure of its Ran-binding domainKarla Langer
Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
PLoS ONE 6:e17011. 2011Proteins bearing a leucine-rich nuclear export signal (NES) are exported from the nucleus by the transport factor CRM1, which forms a cooperative ternary complex with the NES-bearing cargo and with the small GTPase Ran...
- The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathwayJohn H Coyle
Myles H Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
RNA 17:1344-56. 2011..introns, we used reporters in which the mRNA was exported either via the Nxf1/Nxt1 pathway using a CTE or via the Crm1 pathway using Rev/RRE...
- Evolution of a species-specific determinant within human CRM1 that regulates the post-transcriptional phases of HIV-1 replicationNathan M Sherer
Department of Infectious Diseases, King s College London School of Medicine, London, United Kingdom
PLoS Pathog 7:e1002395. 2011..virus type-1 (HIV-1) Rev protein regulates the nuclear export of intron-containing viral RNAs by recruiting the CRM1 nuclear export receptor...
- Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domainsDirk Hoffmann
Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
PLoS ONE 7:e38305. 2012..Subsequently, Rev oligomerizes cooperatively and interacts with the cellular nuclear export receptor CRM1. In addition to mediating nuclear RNA export, Rev also affects the stability, translation and packaging of Rev-..
- Cyclin D1 and mammary carcinomaJ Diehl; Fiscal Year: 2009..Finally, we propose to characterize newly identified cyclin D1 mutants for their ability to undergo active, CRM1-dependent nuclear export...
- Lizabeth A Allison; Fiscal Year: 2015..TR1 follows a cooperative export pathway in which both calreticulin and the export factor CRM1 play a role in facilitating efficient translocation of the receptor from the nucleus to cytoplasm...
- Yuh Min Chook; Fiscal Year: 2016..humans, only three classes of signals for Kap[unreadable]/Kap[unreadable]1, Kap[unreadable]2 (or Transportin) and CRM1, respectively, are known at this time...
- How Karyopherins Move Across The Nuclear Pore ComplexMichael Rexach; Fiscal Year: 2006..and iv) to conduct a comparative study (as delineated for Kap95p) for two additional karyopherins (the exportin Crm1 p, and the importin Kapi 04p) with the goal of uncovering general and specific features in the paths of karyopherin ..
- HTS Assay Development for Modulators of a Nuclear Import/Export SwitchISABELLA GRAEF; Fiscal Year: 2007..between the NFATc family of transcription factors and the karyopherin ?2 (importin?, KPNA2) and exportin (Crm1, XPO1)...
- Screen for Small Molecule Inhibitors of Nuclear Protein ImportLarry Gerace; Fiscal Year: 2007..of small molecule inhibitors of the nuclear transport machinery has been described, which targets the exportin Crm1. The goal of this project is to develop and validate a high-throughput screening (HTS) assay involving ..
- Katherine L B Borden; Fiscal Year: 2016..Our studies revealed that eIF4E dependent mRNA export is distinct from bulk mRNA export, being CRM1 dependent. We will examine the extent to which eIF4E dependent mRNA export uses the CRM1 export machinery...
- Nuclear export of the HIV genome: A Molecular Dynamics Study of the InteractionsMOHAMMAD RK MOFRAD; Fiscal Year: 2013..Nuclear export of the HIV genome: A Molecular Dynamics Study of the Interactions and Structure of HIV Rev and Host CRM1 during Export Summary The HIV virus is a pandemic lentivirus which has no existing cure or vaccine...
- MOLECULAR CHARACTERIZATION OF HERPES SIMPLEX VIRUS ICP27Stephen Rice; Fiscal Year: 2003..We hypothesize that ICP27 interacts with and utilizes two distinct cellular export systems: CRM1 and REF/TAP...
- Structural requirements for the nuclear export of HIV RNADAVID SCOTT BOOTH; Fiscal Year: 2013..an intronic RNA structure called the Rev Response Element (RRE) and by recruiting the host nuclear export adaptors Crm1 and Ran...
- Zijie Sun; Fiscal Year: 2015..Intriguingly, a functional HIV Rev-like leucine rich, CRM1/exportin regulated nuclear export signal (NES) was identified within the C-terminus of LZTS2...
- PATHWAYS OF NUCLEOCYTOPLASMIC TRANSPORTBRYCE PASCHAL; Fiscal Year: 2009..by alanine mutations in AR phosphorylation sites, does not require new protein synthesis or the export receptor Crm1, and is reversible...
- Leptomycin B Development for Cancer TherapyCharles Hutchinson; Fiscal Year: 2004..This microbial metabolite inhibits protein export from the cell nucleus by binding tightly to CRM1 (syn. exportin 1) and inhibiting its ability to shuttle protein complexes across the nuclear membrane...
- Identification of novel co-factors for HIV Tat and Rev as therapeutic targetsAndrew P Rice; Fiscal Year: 2013..The best understood cellular co-factor for Rev is termed XPO1 (CRM1);Rev binds directly to XPO1...
- Rev-dependent Nuclear Export of RNAMirko Hennig; Fiscal Year: 2010..interaction and the subsequent transport of intron-containing viral mRNA mediated by the cellular export receptor CRM1 are essential to the replication of the HIV-1 retrovirus...
- GUANYLATE CYCLASE TRANSDUCTION SYSTEMS IN RETINARameshwar Sharma; Fiscal Year: 2002..involved in GCAPs and S100beta stimulation of ROS-GC have been localized and termed Ca/2+-regulated modules (CRMs): CRM1 is linked to GCAP1 signaling. CRM2 to S100beta and CRM3 to GCAP2...
- REGULATION OF C-REL BY IKB-ALPHMark Hannink; Fiscal Year: 2003..The function of IkappaBalpha as an adaptor protein that bridges c-Rel with the export receptor, Crm1, will be examined...
- Leptomycin B AnalogsYOUNG LI; Fiscal Year: 2005..Many cell signaling processes, including ones essential to the specific onset and development of cancer, rely on CRM1 mediated nuclear export...
- Inhibition of colon tumor progression by Ink4a/ArfGregory Enders; Fiscal Year: 2007....
- Formation of Heterochromatin and Cellular Senescence Driven by HIRA and ASF 1aGregory Enders; Fiscal Year: 2009..Specific Aim 3. Investigate the role of GSK3 activity in localization of HIRA to PML bodies, formation of SAHF and onset of senescence. ..
- G2 Role For Cdk2 in Human CellsGregory Enders; Fiscal Year: 2007..We believe that deregulation of Cdk2, a frequent event in cancer, may contribute to genetic instability and neoplastic progression through deregulation of these functions. ..
- Potent inhibitors of Anthrax Lethal FactorPeter Vogt; Fiscal Year: 2003..Libraries will be targeted using the structural information available for LF, supplemented by information from point mutations of the substrate. Information from our previous synthesis of metalloprotease inhibitors will also be used. ..
- Effect of APOBEC3G on retroviruses and retrotransposonsBRYAN CULLEN; Fiscal Year: 2009..abstract_text> ..
- BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATIONAlan Engelman; Fiscal Year: 2009..abstract_text> ..
- Nuclear Import Pathways for HPV MacromoleculesJUNONA F MOROIANU; Fiscal Year: 2010..Overall, the studies proposed in this renewal application will continue to advance our understanding of major nuclear import pathways relevant to the papillomavirus infection and pathogenesis. ..
- NFKB IN INHIBITION OF CHEMOTHERAPY INDUCED APOPTOSISJames Cusack; Fiscal Year: 2004..abstract_text> ..
- Translational Control of Retroviral Unspliced mRNAKATHLEEN A BORIS LAWRIE; Fiscal Year: 2010..abstract_text> ..
- Drug Abuse and NeuroAIDS in ChinaJohnny He; Fiscal Year: 2008..The social and economic impact can not be overemphasized. The current study seeks to have a better understanding of the prevalence and nature of these diseases in the selected population. [unreadable] [unreadable] [unreadable]..
- THE ONCOGENICITY OF WINGED HELIX PROTEINSPeter Vogt; Fiscal Year: 2003..The nature of activating mutations, the interactions with the preinitiation complex, and the identity and function of target genes will reveal novel molecular targets for therapeutic intervention. ..
- PROTEIN ARGININE METHYLATION AND RNA MATURATIONMichael Henry; Fiscal Year: 2003..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
- Quasispecies Evolution During Lentivirus PersistenceSusan Carpenter; Fiscal Year: 2003..abstract_text> ..
- DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1Mark Wainberg; Fiscal Year: 2001..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
- HIV-1 TROPISM AND CHEMOKINE RECEPTORSBRYAN CULLEN; Fiscal Year: 2002..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
- MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATIONAlan Engelman; Fiscal Year: 2001..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
- The Role of NF-kappaB in Chemotherapy ResistanceJames Cusack; Fiscal Year: 2009..This proposal has the potential to completely alter chemotherapy strategies based on our understanding of the role of NF-kappaB in chemotherapy resistance. ..
- Regulation of histone deacetylase 7 (HDAC7) activityHung Ying Kao; Fiscal Year: 2006..Understanding how cellular signaling controls HDAC activities will help to understand deregulated cell growth in cancer and may have therapeutic implications for cancer treatment. ..
- TARGET-GUIDED DEVELOPMENT OF SPECIFIC AKT INHIBITORSPeter Vogt; Fiscal Year: 2008..4) Investigate the structure-activity relationship through in situ click chemistry and optimize inhibitors for potency, selectivity and cell-based activity ..
- Controlling the Max NetworkPeter Vogt; Fiscal Year: 2008..The proposed work on stabilizers in the Myc-Max network could open the door to a new way of influencing and directing cell growth. ..
- LEDGF-Integrase Structural BiologyAlan Engelman; Fiscal Year: 2005..abstract_text> ..
- Nuclear functions of the tumor suppressor protein APCKRISTI NEUFELD; Fiscal Year: 2008..Greater knowledge of APC function in normal cells will improve our understanding of APC's role in tumorigenesis and ultimately illuminate new points for therapeutic intervention. ..
- MECHANISMS OF HIV-1 ENTRY AND PATHOGENESIS IN ASTROCYTESJohnny He; Fiscal Year: 2008..They should also aid in the development of strategies for treating neurologic symptoms of AIDS patients. ..
- Combinatorial Chemistry and Cancer TargetPeter Vogt; Fiscal Year: 2008..The collaborating groups of this Program Project have become an integrated unit that incorporates a wide range of expertise and interests and applies them to the common goal, new anticancer compounds. ..
- Use a novel Tat transgenic model to develop neuroAIDS therapeuticsJohnny He; Fiscal Year: 2007..The proposed study is to explore the possibility of using a mouse model to develop, evaluate, and validate therapeutic strategies specifically targeted at these diseases. [unreadable] [unreadable] [unreadable]..
- Regulation of human microRNA biosynthesis and activityBRYAN CULLEN; Fiscal Year: 2007..Finally, in specific aim 5, we seek to extend our exciting initial data demonstrating inhibition of miRNA function by the adenovirus VA1 non-coding RNA by trying to define the underlying mechanism. ..
- c-Kit Mutations and Their Role in Tumor BiologyCheryl London; Fiscal Year: 2005..abstract_text> ..
- Molecular Mechanism of HIV-1 Vif FunctionBRYAN CULLEN; Fiscal Year: 2007..Together, this wide-ranging series of experiments should lead to considerable insight into how HIV-1 Vif functions and potentially suggest approaches to inhibit Vif function. ..
- HEPATIC CARCINOGENESIS: MOLECULAR MECHANISMSMartina Buck; Fiscal Year: 2006..abstract_text> ..
- Epo-dependent JAK2 Signaling in Painful NeuropathyWENDY CAMPANA; Fiscal Year: 2006..The experiments will provide new information on the basic mechanisms of nerve injury and pain, and there from, new rationale for development of novel neuroprotective strategies for preventing chronic neuropathic pain. ..
- Tat Interaction with LRP and Its Role in AIDS DementiaJohnny He; Fiscal Year: 2006..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..