XPO1

Summary

Gene Symbol: XPO1
Description: exportin 1
Alias: CRM1, emb, exp1, exportin-1, chromosome region maintenance 1 homolog, chromosome region maintenance 1 protein homolog, exportin 1 (CRM1 homolog, yeast), exportin-1 (required for chromosome region maintenance)
Species: human
Products:     XPO1

Top Publications

  1. Gorlich D, Kutay U. Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol. 1999;15:607-60 pubmed
    ..We discuss mechanistic aspects and the energetics of transport receptor function and describe a number of pathways in detail. ..
  2. Wang W, Budhu A, Forgues M, Wang X. Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. Nat Cell Biol. 2005;7:823-30 pubmed
    ..In addition to nucleocytoplasmic transport, the Ran-Crm1 network is involved in regulating centrosome duplication to ensure the formation of a bipolar spindle...
  3. Lapalombella R, Sun Q, Williams K, Tangeman L, Jha S, Zhong Y, et al. Selective inhibitors of nuclear export show that CRM1/XPO1 is a target in chronic lymphocytic leukemia. Blood. 2012;120:4621-34 pubmed publisher
    The nuclear export protein XPO1 is overexpressed in cancer, leading to the cytoplasmic mislocalization of multiple tumor suppressor proteins...
  4. Arnaoutov A, Azuma Y, Ribbeck K, Joseph J, Boyarchuk Y, Karpova T, et al. Crm1 is a mitotic effector of Ran-GTP in somatic cells. Nat Cell Biol. 2005;7:626-32 pubmed
    ..Here we examine the mitotic function of Crm1, the Ran-GTP-binding nuclear export receptor for leucine-rich cargo (bearing nuclear export sequence) and ..
  5. Koyama M, Matsuura Y. An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1. EMBO J. 2010;29:2002-13 pubmed publisher
    The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES)...
  6. Neville M, Stutz F, Lee L, Davis L, Rosbash M. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export. Curr Biol. 1997;7:767-75 pubmed
    ..Here, we have studied this mechanism of nuclear to cytoplasmic transport. Viable mis-sense mutations in the CRM1 gene substantially reduced or eliminated the biological activity of Rev in S...
  7. Dölker N, Blanchet C, Voß B, Haselbach D, Kappel C, Monecke T, et al. Structural determinants and mechanism of mammalian CRM1 allostery. Structure. 2013;21:1350-60 pubmed publisher
    Proteins carrying nuclear export signals cooperatively assemble with the export factor CRM1 and the effector protein RanGTP...
  8. Shen A, Wang Y, Zhao Y, Zou L, Sun L, Cheng C. Expression of CRM1 in human gliomas and its significance in p27 expression and clinical prognosis. Neurosurgery. 2009;65:153-9; discussion 159-60 pubmed publisher
    ..It is believed that phosphorylation of p27 on Ser10 has been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
  9. Dong X, Biswas A, Süel K, Jackson L, Martinez R, Gu H, et al. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature. 2009;458:1136-41 pubmed publisher
    CRM1 (also known as XPO1 and exportin 1) mediates nuclear export of hundreds of proteins through the recognition of the leucine-rich nuclear export signal (LR-NES). Here we present the 2.9 A structure of CRM1 bound to snurportin 1 (SNUPN)...

More Information

Publications87

  1. Karn J, Stoltzfus C. Transcriptional and posttranscriptional regulation of HIV-1 gene expression. Cold Spring Harb Perspect Med. 2012;2:a006916 pubmed publisher
    ..In cells that are not fully activated, limiting levels of Tat and Rev act as potent blocks to premature virus production. ..
  2. Guttler T, Madl T, Neumann P, Deichsel D, Corsini L, Monecke T, et al. NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Nat Struct Mol Biol. 2010;17:1367-76 pubmed publisher
    Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs...
  3. McBride K, Barreto V, Ramiro A, Stavropoulos P, Nussenzweig M. Somatic hypermutation is limited by CRM1-dependent nuclear export of activation-induced deaminase. J Exp Med. 2004;199:1235-44 pubmed
    ..Here we show that AID is actively excluded from the nucleus by an exportin CRM1-dependent pathway...
  4. Prechtel A, Chemnitz J, Schirmer S, Ehlers C, Langbein Detsch I, Stülke J, et al. Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. J Biol Chem. 2006;281:10912-25 pubmed
    ..In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN ..
  5. Yedavalli V, Neuveut C, Chi Y, Kleiman L, Jeang K. Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function. Cell. 2004;119:381-92 pubmed
    ..Previously, CRM1 was identified as a cellular cofactor for Rev-dependent export of intron-containing HIV-1 RNA...
  6. Ranganathan P, Yu X, Na C, Santhanam R, Shacham S, Kauffman M, et al. Preclinical activity of a novel CRM1 inhibitor in acute myeloid leukemia. Blood. 2012;120:1765-73 pubmed publisher
    Chromosome maintenance protein 1 (CRM1) is a nuclear export receptor involved in the active transport of tumor suppressors (e.g...
  7. He J, Henao Mejia J, Liu Y. Sam68 functions in nuclear export and translation of HIV-1 RNA. RNA Biol. 2009;6:384-6 pubmed
    ..Elucidation of the precise molecular function of Sam68 in HIV-1 gene expression is clearly warranted and is expected to unambiguously establish Sam68 as an important host factor for HIV-1 replication. ..
  8. Englmeier L, Fornerod M, Bischoff F, Petosa C, Mattaj I, Kutay U. RanBP3 influences interactions between CRM1 and its nuclear protein export substrates. EMBO Rep. 2001;2:926-32 pubmed
    We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP...
  9. Yao Y, Dong Y, Lin F, Zhao H, Shen Z, Chen P, et al. The expression of CRM1 is associated with prognosis in human osteosarcoma. Oncol Rep. 2009;21:229-35 pubmed
    The nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) is involved in the nuclear export of proteins and messenger RNAs and, thus, mediates the subcellular distribution of important molecules...
  10. Mao L, Yang Y. Targeting the nuclear transport machinery by rational drug design. Curr Pharm Des. 2013;19:2318-25 pubmed
    CRM1 (also referred as exportin1 or Xpo1) is a key member of the importin ? superfamily of nuclear transport receptors. Its potential as therapeutic target has attracted significant attention in recent years...
  11. Elinav H, Wu Y, Coskun A, Hryckiewicz K, Kemler I, Hu Y, et al. Human CRM1 augments production of infectious human and feline immunodeficiency viruses from murine cells. J Virol. 2012;86:12053-68 pubmed publisher
    ..In contrast, the overexpression of mouse CRM1 (mCRM1) minimally affected HIV-1 and FIV production and did not antagonize hCRM1...
  12. Williams P, Verhagen J, Elliott G. Characterization of a CRM1-dependent nuclear export signal in the C terminus of herpes simplex virus type 1 tegument protein UL47. J Virol. 2008;82:10946-52 pubmed publisher
    ..Finally, we show that the hUL47 NES is sensitive to the inhibitor of CRM1-mediated nuclear export leptomycin B...
  13. Dian C, Bernaudat F, Langer K, Oliva M, Fornerod M, Schoehn G, et al. Structure of a truncation mutant of the nuclear export factor CRM1 provides insights into the auto-inhibitory role of its C-terminal helix. Structure. 2013;21:1338-49 pubmed publisher
    Chromosome region maintenance 1/exportin1/Xpo1 (CRM1) associates with the GTPase Ran to mediate the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES)...
  14. Trotta C, Lund E, Kahan L, Johnson A, Dahlberg J. Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates. EMBO J. 2003;22:2841-51 pubmed
    ..We show that in vertebrate cells, transport of both subunits requires the export receptor CRM1 and Ran.GTP. Export of 60S subunits is coupled with that of the nucleo- cytoplasmic shuttling protein NMD3...
  15. Petosa C, Schoehn G, Askjaer P, Bauer U, Moulin M, Steuerwald U, et al. Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex. Mol Cell. 2004;16:761-75 pubmed
    b>CRM1/Exportin1 mediates the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran...
  16. Dupont S, Sharova N, DeHoratius C, Virbasius C, Zhu X, Bukrinskaya A, et al. A novel nuclear export activity in HIV-1 matrix protein required for viral replication. Nature. 1999;402:681-5 pubmed
    ..We conclude that the MA nuclear export signal is required to counteract the MA nuclear localization signal, thus ensuring the cytoplasmic availability of the components required for virion assembly. ..
  17. Hakata Y, Yamada M, Shida H. A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein. Mol Cell Biol. 2003;23:8751-61 pubmed
    Human CRM1 (hCRM1) functions in the Rex-mediated mRNA export of human T-cell leukemia virus type 1 (HTLV-1) as an export receptor and as an inducing factor for Rex multimerization on its cognate RNA...
  18. Forgues M, Difilippantonio M, Linke S, Ried T, Nagashima K, Feden J, et al. Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindles. Mol Cell Biol. 2003;23:5282-92 pubmed
    ..We recently showed that HBx induces cytoplasmic sequestration of Crm1, a nuclear export receptor that binds to Ran GTPase, thereby inducing nuclear localization of NF-kappaB...
  19. Stauber R, Mann W, Knauer S. Nuclear and cytoplasmic survivin: molecular mechanism, prognostic, and therapeutic potential. Cancer Res. 2007;67:5999-6002 pubmed
    ..Recent evidence shows that the direct interaction of survivin with the nuclear export receptor Crm1 is critically involved in its intracellular localization and cancer-relevant functions...
  20. Fischer U, Huber J, Boelens W, Mattaj I, Luhrmann R. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell. 1995;82:475-83 pubmed
    ..BSA-R was itself exported from the nucleus in an active, saturable manner. Thus, the Rev activation domain constitutes a nuclear export signal that redirects RRE-containing viral RNAs to a non-mRNA export pathway. ..
  21. van der Watt P, Maske C, Hendricks D, Parker M, Denny L, Govender D, et al. The Karyopherin proteins, Crm1 and Karyopherin beta1, are overexpressed in cervical cancer and are critical for cancer cell survival and proliferation. Int J Cancer. 2009;124:1829-40 pubmed publisher
    ..In this study, we examined the expression of the Karyopherins, Crm1, Karyopherin beta1 (Kpnbeta1) and Karyopherin alpha2 (Kpnalpha2), in cervical tissue and cell lines...
  22. Ohno M, Segref A, Bachi A, Wilm M, Mattaj I. PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation. Cell. 2000;101:187-98 pubmed
    ..Export depends upon the RNA cap structure, nuclear cap-binding complex (CBC), the export receptor CRM1/Xpo1, and RanGTP. These components are however insufficient to support U snRNA export...
  23. Fornerod M, Ohno M, Yoshida M, Mattaj I. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell. 1997;90:1051-60 pubmed
    b>CRM1 is distantly related to receptors that mediate nuclear protein import and was previously shown to interact with the nuclear pore complex. Overexpression of CRM1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus...
  24. Kudo N, Khochbin S, Nishi K, Kitano K, Yanagida M, Yoshida M, et al. Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins. J Biol Chem. 1997;272:29742-51 pubmed
    b>Crm1 of Schizosaccharomyces pombe, a nuclear protein essential for proliferation and chromosome region maintenance, is a possible target of leptomycin B, an antifungal and antitumor antibiotic with cell cycle-arresting activity...
  25. Shida H. Role of Nucleocytoplasmic RNA Transport during the Life Cycle of Retroviruses. Front Microbiol. 2012;3:179 pubmed publisher
    ..encode the regulatory proteins Rev and Rex, which form a bridge between the viral RNA and the export receptor CRM1. Recent studies show that these transport systems are not only involved in RNA export, but also in the ..
  26. Nemergut M, Lindsay M, Brownawell A, Macara I. Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. J Biol Chem. 2002;277:17385-8 pubmed
    Ran-binding protein 3 (RanBP3) is an approximately 55-kDa protein that functions as a cofactor for Crm1-mediated nuclear export. RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran.GTP and cargo...
  27. Bischoff F, Krebber H, Kempf T, Hermes I, Ponstingl H. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc Natl Acad Sci U S A. 1995;92:1749-53 pubmed
    ..Thus, a defect in either of the two antagonistic regulators of Ran would result in a shutdown of Ran-dependent transport processes, in agreement with the almost identical phenotypes described for such defects in budding yeast. ..
  28. Plafker K, Macara I. Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1. Mol Cell Biol. 2000;20:3510-21 pubmed
    ..Shuttling of RanBP1 may function to clear nuclear pores of Ran:GTP, to prevent premature release of import cargo from transport receptors. ..
  29. Fox A, Ciziene D, McLaughlin S, Stewart M. Electrostatic interactions involving the extreme C terminus of nuclear export factor CRM1 modulate its affinity for cargo. J Biol Chem. 2011;286:29325-35 pubmed publisher
    The toroid-shaped nuclear protein export factor CRM1 is constructed from 21 tandem HEAT repeats, each of which contains an inner (B) and outer (A) ?-helix joined by loops...
  30. Coyle J, Bor Y, Rekosh D, Hammarskjold M. The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway. RNA. 2011;17:1344-56 pubmed publisher
    ..introns, we used reporters in which the mRNA was exported either via the Nxf1/Nxt1 pathway using a CTE or via the Crm1 pathway using Rev/RRE...
  31. Hofmann W, Reichart B, Ewald A, Muller E, Schmitt I, Stauber R, et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin. J Cell Biol. 2001;152:895-910 pubmed
    Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the export receptor CRM1/exportin1. However, additional protein factors interacting with leucine-rich NESs have been described...
  32. Lindsay M, Holaska J, Welch K, Paschal B, Macara I. Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export. J Cell Biol. 2001;153:1391-402 pubmed
    b>Crm1 is a member of the karyopherin family of nucleocytoplasmic transport receptors and mediates the export of proteins from the nucleus by forming a ternary complex with cargo and Ran:GTP...
  33. Yang J, Bogerd H, Wang P, Page D, Cullen B. Two closely related human nuclear export factors utilize entirely distinct export pathways. Mol Cell. 2001;8:397-406 pubmed
    ..NXF3 contains a novel Crm1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain...
  34. Sherer N, Swanson C, Hue S, Roberts R, Bergeron J, Malim M. Evolution of a species-specific determinant within human CRM1 that regulates the post-transcriptional phases of HIV-1 replication. PLoS Pathog. 2011;7:e1002395 pubmed publisher
    ..virus type-1 (HIV-1) Rev protein regulates the nuclear export of intron-containing viral RNAs by recruiting the CRM1 nuclear export receptor...
  35. Suhasini M, Reddy T. Cellular proteins and HIV-1 Rev function. Curr HIV Res. 2009;7:91-100 pubmed
    ..While a number of reviews have focused on the host proteins (i.e., Crm1, importin-beta and nucleoporins) that specifically regulate shuttling of Rev between the nucleus and cytoplasm, we ..
  36. Aligeti M, Behrens R, Pocock G, Schindelin J, Dietz C, Eliceiri K, et al. Cooperativity among Rev-associated nuclear export signals regulates HIV-1 gene expression and is a determinant of virus species tropism. J Virol. 2014;88:14207-21 pubmed publisher
    ..of the chromosomal region maintenance 1 (mCRM1) nuclear export receptor and rescued by the expression of human CRM1 (hCRM1)...
  37. Zhao C, Mahboobi S, Moussavi Baygi R, Mofrad M. The interaction of CRM1 and the nuclear pore protein Tpr. PLoS ONE. 2014;9:e93709 pubmed publisher
    ..Recent work has demonstrated a binding interaction between the exportin CRM1 and the unstructured carboxylic tail of Tpr, on the nuclear basket...
  38. Hantschel O, Wiesner S, Guttler T, Mackereth C, Rix L, Mikes Z, et al. Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl. Mol Cell. 2005;19:461-73 pubmed
    ..We propose that these interactions represent a major determinant for both Bcr-Abl and c-Abl localization. ..
  39. Patenaude A, Orthwein A, Hu Y, Campo V, Kavli B, Buschiazzo A, et al. Active nuclear import and cytoplasmic retention of activation-induced deaminase. Nat Struct Mol Biol. 2009;16:517-27 pubmed publisher
    ..Blocking nuclear import alters the balance between these processes in favor of cytoplasmic retention, resulting in reduced isotype class switching. ..
  40. Xu D, Farmer A, Collett G, Grishin N, Chook Y. Sequence and structural analyses of nuclear export signals in the NESdb database. Mol Biol Cell. 2012;23:3677-93 pubmed publisher
    We compiled >200 nuclear export signal (NES)-containing CRM1 cargoes in a database named NESdb...
  41. Okada H, Zhang X, Ben Fofana I, Nagai M, Suzuki H, Ohashi T, et al. Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages. Retrovirology. 2009;6:43 pubmed publisher
    ..basis for developing a better rat model for HIV-1 infection, we evaluated the effect of human CyclinT1 (hCycT1) and CRM1 (hCRM1) on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells ..
  42. Yedavalli V, Jeang K. Rev-ing up post-transcriptional HIV-1 RNA expression. RNA Biol. 2011;8:195-9 pubmed
    ..Here, the post-transcriptional activities of Rev are discussed in the context of a recent finding that an RNA cap methyltransferase contributes to the expression of unspliced / partially spliced HIV-1 transcripts. ..
  43. Nagai Fukataki M, Ohashi T, Hashimoto I, Kimura T, Hakata Y, Shida H. Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells. Genes Cells. 2011;16:203-16 pubmed publisher
    ..the nuclear export of unspliced gag and singly spliced env mRNAs by bridging viral RNA and the export receptor, CRM1. Recently, rat CRM1 was found to be less efficient than human CRM1 in supporting Rev function in rats...
  44. Nekhai S, Jeang K. Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev. Future Microbiol. 2006;1:417-26 pubmed
    ..Rev primarily functions to export unspliced and partially spliced viral RNAs from the nucleus into the cytoplasm. For this activity, Rev cooperates with cellular transport protein CRM1 and RNA helicases DDX1 and DDX3, amongst others.
  45. Bogerd H, Fridell R, Madore S, Cullen B. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins. Cell. 1995;82:485-94 pubmed
    ..We conclude that Rab is the predicted activation domain-specific cofactor for the Rev/Rex class of RNA export factors. ..
  46. Kehlenbach R, Dickmanns A, Kehlenbach A, Guan T, Gerace L. A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export. J Cell Biol. 1999;145:645-57 pubmed
    ..activated T cells) can be reconstituted in permeabilized cells with the GTPase Ran and the nuclear export receptor CRM1. We have now used this assay to identify another export factor...
  47. Kudo N, Matsumori N, Taoka H, Fujiwara D, Schreiner E, Wolff B, et al. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc Natl Acad Sci U S A. 1999;96:9112-7 pubmed
    The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins...
  48. Guan T, Kehlenbach R, Schirmer E, Kehlenbach A, Fan F, Clurman B, et al. Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export. Mol Cell Biol. 2000;20:5619-30 pubmed
    ..Correspondingly, CRM1, the export receptor for leucine-rich export sequences, directly bound to a fragment of Nup50 in vitro, whereas ..
  49. Yi R, Bogerd H, Cullen B. Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAs. J Virol. 2002;76:2036-42 pubmed
    ..General agreement exists that Rev acts, at least in part, by recruiting the cellular Crm1 nuclear export factor to HIV-1 transcripts bearing the Rev response element RNA target, and thereby inducing their ..
  50. Ishida N, Hara T, Kamura T, Yoshida M, Nakayama K, Nakayama K. Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export. J Biol Chem. 2002;277:14355-8 pubmed
    ..Phosphorylation of p27(Kip1) on Ser(10) has now been shown to be required for the binding of CRM1, a carrier protein for nuclear export...
  51. Noske A, Weichert W, Niesporek S, Röske A, Buckendahl A, Koch I, et al. Expression of the nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 is a prognostic factor in human ovarian cancer. Cancer. 2008;112:1733-43 pubmed publisher
    The human nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) mediates the nuclear export of proteins and messenger RNAs and, thus, is an important regulator of subcellular distribution of key molecules...
  52. Costes S, Daelemans D, Cho E, Dobbin Z, Pavlakis G, Lockett S. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J. 2004;86:3993-4003 pubmed
    ..further illustrated by measuring, for the first time in live cells, the dissociation rate (k(d)) of the HIV-1 Rev/CRM1 export complex induced by the cytotoxin leptomycin B...
  53. Rosorius O, Reichart B, Kratzer F, Heger P, Dabauvalle M, Hauber J. Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1. J Cell Sci. 1999;112 ( Pt 14):2369-80 pubmed
    ..We are able to show that eIF-5A interacts with the general nuclear export receptor, CRM1. Furthermore, microinjection studies in somatic cells revealed that eIF-5A is transported from the nucleus to the ..
  54. Fornerod M, van Deursen J, van Baal S, Reynolds A, Davis D, Murti K, et al. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J. 1997;16:807-16 pubmed
    ..The 112 kDa protein is the human homologue of yeast CRM1, a protein known to be required for maintenance of correct chromosome structure...
  55. Ossareh Nazari B, Bachelerie F, Dargemont C. Evidence for a role of CRM1 in signal-mediated nuclear protein export. Science. 1997;278:141-4 pubmed
    Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (..
  56. Tai Y, Landesman Y, Acharya C, Calle Y, Zhong M, Cea M, et al. CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications. Leukemia. 2014;28:155-65 pubmed publisher
    The key nuclear export protein CRM1/XPO1 may represent a promising novel therapeutic target in human multiple myeloma (MM)...
  57. Inoue H, Kauffman M, Shacham S, Landesman Y, Yang J, Evans C, et al. CRM1 blockade by selective inhibitors of nuclear export attenuates kidney cancer growth. J Urol. 2013;189:2317-26 pubmed publisher
    ..We identified and evaluated the nuclear exporter protein CRM1 as a novel potential therapy for renal cell carcinoma...
  58. Paraskeva E, Izaurralde E, Bischoff F, Huber J, Kutay U, Hartmann E, et al. CRM1-mediated recycling of snurportin 1 to the cytoplasm. J Cell Biol. 1999;145:255-64 pubmed
    ..We have shown previously that CAS mediates export of importin alpha. Here we show that snurportin 1 is exported by CRM1, the receptor for leucine-rich nuclear export signals (NESs)...
  59. Dong X, Biswas A, Chook Y. Structural basis for assembly and disassembly of the CRM1 nuclear export complex. Nat Struct Mol Biol. 2009;16:558-60 pubmed publisher
    CRM1 (or exportin 1, Xpo1) transports proteins out of the cell nucleus through the nuclear pore complex...
  60. Pathria G, Wagner C, Wagner S. Inhibition of CRM1-mediated nucleocytoplasmic transport: triggering human melanoma cell apoptosis by perturbing multiple cellular pathways. J Invest Dermatol. 2012;132:2780-90 pubmed publisher
    ..DNA microarray analysis, we identified four key components of the nucleocytoplasmic transport machinery-CRM1, RAN (RAN-GTPase), RANGAP1, and RANBP1-to be overexpressed in human melanoma metastases...
  61. Yang J, Bogerd H, Peng S, Wiegand H, Truant R, Cullen B. An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein. Proc Natl Acad Sci U S A. 1999;96:13404-8 pubmed
    ..Like HIV-1 Rev, K-Rev binds to both the Crm1 nuclear export factor and to a cis-acting viral RNA target to activate nuclear export of unspliced RNAs...
  62. Bachi A, Braun I, Rodrigues J, Pante N, Ribbeck K, von Kobbe C, et al. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. RNA. 2000;6:136-58 pubmed
  63. Daugherty M, Liu B, Frankel A. Structural basis for cooperative RNA binding and export complex assembly by HIV Rev. Nat Struct Mol Biol. 2010;17:1337-42 pubmed publisher
    ..the Rev response element (RRE) RNA, to form a highly specific oligomeric ribonucleoprotein (RNP) that binds to the Crm1 host export factor...
  64. Hakata Y, Yamada M, Mabuchi N, Shida H. The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions. J Virol. 2002;76:8079-89 pubmed
    ..the nuclear export of unspliced and singly spliced viral mRNAs by bridging viral RNA and export receptor human CRM1 (hCRM1)...
  65. Elton D, Simpson Holley M, Archer K, Medcalf L, Hallam R, McCauley J, et al. Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway. J Virol. 2001;75:408-19 pubmed
    ..We found that the drug leptomycin B (LMB), which specifically inactivates the cellular CRM1 polypeptide, caused nuclear retention of NP in virus-infected cells, indicating a role for the CRM1 nuclear export ..
  66. Langer K, Dian C, Rybin V, Müller C, Petosa C. Insights into the function of the CRM1 cofactor RanBP3 from the structure of its Ran-binding domain. PLoS ONE. 2011;6:e17011 pubmed publisher
    Proteins bearing a leucine-rich nuclear export signal (NES) are exported from the nucleus by the transport factor CRM1, which forms a cooperative ternary complex with the NES-bearing cargo and with the small GTPase Ran...
  67. Kimura T, Hashimoto I, Nishikawa M, Yamada H. Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase. Med Mol Morphol. 2009;42:70-81 pubmed publisher
    ..The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export...
  68. Huang W, Yue L, Qiu W, Wang L, Zhou X, Sun Y. Prognostic value of CRM1 in pancreas cancer. Clin Invest Med. 2009;32:E315 pubmed
    ..This study investigated the prognostic value of nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) expression in pancreas cancer...
  69. Cmarko D, Bøe S, Scassellati C, Szilvay A, Davanger S, Fu X, et al. Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs. J Virol. 2002;76:10473-84 pubmed
    ..The simultaneous demonstration of Rev, Tat, Br-labeled RNA, and cellular proteins SC35 and CRM1 in such fibrils reveals the potential of Rev to associate with nascent HIV pre-mRNA and its splicing complex and ..
  70. Stade K, Ford C, Guthrie C, Weis K. Exportin 1 (Crm1p) is an essential nuclear export factor. Cell. 1997;90:1041-50 pubmed
    ..cerevisiae. The presented data are consistent with the conclusion that Crm1p is a carrier for the NES-mediated protein export pathway. We propose CRM1 be renamed exportin 1 (XPO1).
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    Chromosomal region maintenance 1 (CRM1) is a nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal. CRM1 is involved in nuclear export of tumor suppressors such as p53...
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    ..In particular, Nullbasic induced redistribution of exportin 1 (CRM1), nucleophosmin (B23) and nucleolin (C23) from the nucleolus to the nucleus when Rev was coexpressed, but ..
  73. Askjaer P, Jensen T, Nilsson J, Englmeier L, Kjems J. The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP. J Biol Chem. 1998;273:33414-22 pubmed
    ..The cellular CRM1 (Exportin1) protein functions as a nuclear export receptor for proteins carrying a Rev-like NES in a process that ..
  74. Floer M, Blobel G. Putative reaction intermediates in Crm1-mediated nuclear protein export. J Biol Chem. 1999;274:16279-86 pubmed
    We discovered several novel interactions between proteins involved in Crm1-mediated nuclear export of the nuclear export signal containing human immunodeficiency virus type 1 protein Rev...
  75. Daelemans D, Costes S, Lockett S, Pavlakis G. Kinetic and molecular analysis of nuclear export factor CRM1 association with its cargo in vivo. Mol Cell Biol. 2005;25:728-39 pubmed
    The nucleocytoplasmic transport receptor CRM1 mediates the export of macromolecules from the nucleus to the cytoplasm by forming a ternary complex with a cargo molecule and RanGTP...
  76. Thomas F, Kutay U. Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway. J Cell Sci. 2003;116:2409-19 pubmed
    ..Most nuclear export pathways require RanGTP-binding export receptors. We analyzed the role of CRM1, the export receptor for leucine-rich nuclear export signals (NES), in the biogenesis of ribosomal subunits in ..
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    Rat ortholog of human CRM1 has been found to be responsible for the poor activity of viral Rex protein, which is essential for RNA export of human T cell leukemia virus type 1 (HTLV-1)...
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    ..Subsequently, Rev oligomerizes cooperatively and interacts with the cellular nuclear export receptor CRM1. In addition to mediating nuclear RNA export, Rev also affects the stability, translation and packaging of Rev-..