WNT3A

Summary

Gene Symbol: WNT3A
Description: Wnt family member 3A
Alias: protein Wnt-3a, wingless-type MMTV integration site family, member 3A
Species: human
Products:     WNT3A

Top Publications

  1. Davidson K, Jamshidi P, Daly R, Hearn M, Pera M, Dottori M. Wnt3a regulates survival, expansion, and maintenance of neural progenitors derived from human embryonic stem cells. Mol Cell Neurosci. 2007;36:408-15 pubmed
    ..In addition, neurosphere formation and size were significantly greater in the presence of exogenous Wnt3a compared to control conditions...
  2. Tauriello D, Haegebarth A, Kuper I, Edelmann M, Henraat M, Canninga Van Dijk M, et al. Loss of the tumor suppressor CYLD enhances Wnt/beta-catenin signaling through K63-linked ubiquitination of Dvl. Mol Cell. 2010;37:607-19 pubmed publisher
    ..Together, our results argue that loss of CYLD instigates tumor growth in human cylindromatosis through a mechanism in which hyperubiquitination of polymerized Dvl drives enhancement of Wnt responses...
  3. Gazit A, Yaniv A, Bafico A, Pramila T, Igarashi M, Kitajewski J, et al. Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response. Oncogene. 1999;18:5959-66 pubmed
    ..All of these findings provide strong evidence that Hfz1 is a functional partner for certain Wnts in inducing TCF dependent transcription. ..
  4. Lu W, Yamamoto V, Ortega B, Baltimore D. Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth. Cell. 2004;119:97-108 pubmed
    ..Thus, Ryk appears to play a crucial role in Wnt-mediated signaling. ..
  5. Vijayaragavan K, Szabo E, Bosse M, Ramos Mejia V, Moon R, Bhatia M. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell. 2009;4:248-62 pubmed publisher
    ..We demonstrate that activation of canonical Wnt signaling by Wnt3a promotes proliferation of human embryonic stem cells (hESCs)--precursors already committed to the hematopoietic ..
  6. Nalesso G, Sherwood J, Bertrand J, Pap T, Ramachandran M, De Bari C, et al. WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways. J Cell Biol. 2011;193:551-64 pubmed publisher
    ..We propose a novel model in which a single WNT can simultaneously activate different pathways with distinct and independent outcomes and with reciprocal regulation. This offers an opportunity for selective pharmacological targeting...
  7. Liu J, Ding X, Tang J, Cao Y, Hu P, Zhou F, et al. Enhancement of canonical Wnt/?-catenin signaling activity by HCV core protein promotes cell growth of hepatocellular carcinoma cells. PLoS ONE. 2011;6:e27496 pubmed publisher
    ..HCV core protein significantly enhances Tcf-dependent transcriptional activity induced by Wnt3A in HCC cell lines...
  8. Nygren M, Døsen Dahl G, Stubberud H, Walchli S, Munthe E, Rian E. beta-catenin is involved in N-cadherin-dependent adhesion, but not in canonical Wnt signaling in E2A-PBX1-positive B acute lymphoblastic leukemia cells. Exp Hematol. 2009;37:225-33 pubmed publisher
    ..Instead, beta-catenin is involved in N-cadherin-dependent adherence junctions, suggesting for the first time that leukemia-stroma interactions may be mediated via an N-cadherin-dependent mechanism. ..
  9. Chien A, Moore E, Lonsdorf A, Kulikauskas R, Rothberg B, Berger A, et al. Activated Wnt/beta-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model. Proc Natl Acad Sci U S A. 2009;106:1193-8 pubmed publisher
    ..vivo is recapitulated in B16 murine melanoma cells and in human melanoma cell lines cultured in vitro with either WNT3A or small-molecule activators of beta-catenin signaling...
  10. Li X, Chen C, Wang F, Huang W, Liang Z, Xiao Y, et al. KCTD1 suppresses canonical Wnt signaling pathway by enhancing ?-catenin degradation. PLoS ONE. 2014;9:e94343 pubmed publisher
    ..Finally, we found that Wnt pathway member APC and tumor suppressor p53 influence KCTD1-mediated downregulation of ?-catenin. These results suggest that KCTD1 functions as a novel inhibitor of Wnt signaling pathway. ..

Detail Information

Publications110 found, 100 shown here

  1. Davidson K, Jamshidi P, Daly R, Hearn M, Pera M, Dottori M. Wnt3a regulates survival, expansion, and maintenance of neural progenitors derived from human embryonic stem cells. Mol Cell Neurosci. 2007;36:408-15 pubmed
    ..In addition, neurosphere formation and size were significantly greater in the presence of exogenous Wnt3a compared to control conditions...
  2. Tauriello D, Haegebarth A, Kuper I, Edelmann M, Henraat M, Canninga Van Dijk M, et al. Loss of the tumor suppressor CYLD enhances Wnt/beta-catenin signaling through K63-linked ubiquitination of Dvl. Mol Cell. 2010;37:607-19 pubmed publisher
    ..Together, our results argue that loss of CYLD instigates tumor growth in human cylindromatosis through a mechanism in which hyperubiquitination of polymerized Dvl drives enhancement of Wnt responses...
  3. Gazit A, Yaniv A, Bafico A, Pramila T, Igarashi M, Kitajewski J, et al. Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response. Oncogene. 1999;18:5959-66 pubmed
    ..All of these findings provide strong evidence that Hfz1 is a functional partner for certain Wnts in inducing TCF dependent transcription. ..
  4. Lu W, Yamamoto V, Ortega B, Baltimore D. Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth. Cell. 2004;119:97-108 pubmed
    ..Thus, Ryk appears to play a crucial role in Wnt-mediated signaling. ..
  5. Vijayaragavan K, Szabo E, Bosse M, Ramos Mejia V, Moon R, Bhatia M. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell. 2009;4:248-62 pubmed publisher
    ..We demonstrate that activation of canonical Wnt signaling by Wnt3a promotes proliferation of human embryonic stem cells (hESCs)--precursors already committed to the hematopoietic ..
  6. Nalesso G, Sherwood J, Bertrand J, Pap T, Ramachandran M, De Bari C, et al. WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways. J Cell Biol. 2011;193:551-64 pubmed publisher
    ..We propose a novel model in which a single WNT can simultaneously activate different pathways with distinct and independent outcomes and with reciprocal regulation. This offers an opportunity for selective pharmacological targeting...
  7. Liu J, Ding X, Tang J, Cao Y, Hu P, Zhou F, et al. Enhancement of canonical Wnt/?-catenin signaling activity by HCV core protein promotes cell growth of hepatocellular carcinoma cells. PLoS ONE. 2011;6:e27496 pubmed publisher
    ..HCV core protein significantly enhances Tcf-dependent transcriptional activity induced by Wnt3A in HCC cell lines...
  8. Nygren M, Døsen Dahl G, Stubberud H, Walchli S, Munthe E, Rian E. beta-catenin is involved in N-cadherin-dependent adhesion, but not in canonical Wnt signaling in E2A-PBX1-positive B acute lymphoblastic leukemia cells. Exp Hematol. 2009;37:225-33 pubmed publisher
    ..Instead, beta-catenin is involved in N-cadherin-dependent adherence junctions, suggesting for the first time that leukemia-stroma interactions may be mediated via an N-cadherin-dependent mechanism. ..
  9. Chien A, Moore E, Lonsdorf A, Kulikauskas R, Rothberg B, Berger A, et al. Activated Wnt/beta-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model. Proc Natl Acad Sci U S A. 2009;106:1193-8 pubmed publisher
    ..vivo is recapitulated in B16 murine melanoma cells and in human melanoma cell lines cultured in vitro with either WNT3A or small-molecule activators of beta-catenin signaling...
  10. Li X, Chen C, Wang F, Huang W, Liang Z, Xiao Y, et al. KCTD1 suppresses canonical Wnt signaling pathway by enhancing ?-catenin degradation. PLoS ONE. 2014;9:e94343 pubmed publisher
    ..Finally, we found that Wnt pathway member APC and tumor suppressor p53 influence KCTD1-mediated downregulation of ?-catenin. These results suggest that KCTD1 functions as a novel inhibitor of Wnt signaling pathway. ..
  11. Chen Y, Huang Q, Zhou H, Wang Y, Hu X, Li T. Inhibition of canonical WNT/β-catenin signaling is involved in leflunomide (LEF)-mediated cytotoxic effects on renal carcinoma cells. Oncotarget. 2016;7:50401-50416 pubmed publisher
    ..However, LEF treatment also triggered cell autophagy and elevated the expression of WNT3a, which ameliorated its cytotoxic effects...
  12. Sakisaka Y, Kanaya S, Nakamura T, Tamura M, Shimauchi H, Nemoto E. p38 MAP kinase is required for Wnt3a-mediated osterix expression independently of Wnt-LRP5/6-GSK3? signaling axis in dental follicle cells. Biochem Biophys Res Commun. 2016;478:527-32 pubmed publisher
    b>Wnt3a is a secreted glycoprotein that activates the glycogen synthase kinase-3? (GSK3?)/?-catenin signaling pathway through low-density-lipoprotein receptor-related protein (LRP)5/6 co-receptors...
  13. Lin H, Angeli M, Chung K, Ejimadu C, Rosa A, Lee T. sFRP2 activates Wnt/?-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling. Am J Physiol Cell Physiol. 2016;311:C710-C719 pubmed publisher
    ..activates CFs in part through canonical Wnt/?-catenin signaling, as evidenced by increased expression of Axin2 and Wnt3a, but not Wnt5a, as well as accumulation of nuclear ?-catenin...
  14. Luo L, Luo G, Fang Q, Huang S, Chen H. [The regulatory role of Wnt signaling pathway in the proliferation and activation of memory T cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1595-1599 pubmed
    ..The expression levels of Wnt3a and Wnt5a were significantly higher in mDCs than in imDCs...
  15. Teofili L, Martini M, Nuzzolo E, Capodimonti S, Iachininoto M, Cocomazzi A, et al. Endothelial progenitor cell dysfunction in myelodysplastic syndromes: possible contribution of a defective vascular niche to myelodysplasia. Neoplasia. 2015;17:401-9 pubmed publisher
    ..The addition of Wnt3A reduced the expression of intercellular cell adhesion molecule-1 on MDS ECFCs and restored the defective ..
  16. Carthy J, Engström U, Heldin C, Moustakas A. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling. J Cell Biochem. 2016;117:938-45 pubmed publisher
    ..non-Wnt related signaling activities which were present in commercially available preparations of recombinant Wnt3a. Specifically, we found that treatment of cultured fibroblasts with recombinant Wnt3a induced immediate activation ..
  17. Shin H, Islam R, Yoon W, Lee T, Cho Y, Bae H, et al. Pin1-mediated Modification Prolongs the Nuclear Retention of β-Catenin in Wnt3a-induced Osteoblast Differentiation. J Biol Chem. 2016;291:5555-65 pubmed publisher
    ..These results indicate that Pin1 could be a critical target to modulate β-catenin-mediated osteogenesis. ..
  18. Mimura S, Suga M, Okada K, Kinehara M, Nikawa H, Furue M. Bone morphogenetic protein 4 promotes craniofacial neural crest induction from human pluripotent stem cells. Int J Dev Biol. 2016;60:21-8 pubmed publisher
    ..morphogenetic protein (BMP) 4 in defined serum-free culture conditions including fibroblast growth factor-2 and Wnt3a from day 8 after NC specification induced the expression of cranial NC markers, AP2alpha, MSX1, and DLX1, during ..
  19. Zheng H, Jia L, Liu C, Rong Z, Zhong L, Yang L, et al. TREM2 Promotes Microglial Survival by Activating Wnt/?-Catenin Pathway. J Neurosci. 2017;37:1772-1784 pubmed publisher
    ..More importantly, treatment with Wnt3a, LiCl, or TDZD-8, which activates the ?-catenin-mediated Wnt signaling pathway, rescued microglia survival and ..
  20. Xing Y, Chen X, Cao Y, Huang J, Xie X, Wei Y. Expression of Wnt and Notch signaling pathways in inflammatory bowel disease treated with mesenchymal stem cell transplantation: evaluation in a rat model. Stem Cell Res Ther. 2015;6:101 pubmed publisher
    ..39±0.54) and the normal group (1.62±0.25) (P <0.05). The Wnt3a mRNA was more highly expressed in IBD rats (2.92±0.94) and decreased in MSCT rats (0.17±0.63, P <0.05)...
  21. Khalil H, Lei N, Brinkley G, Scott A, Wang J, Kar U, et al. A novel culture system for adult porcine intestinal crypts. Cell Tissue Res. 2016;365:123-34 pubmed publisher
    ..epidermal growth factor, noggin, and R-spondin 1 (ENR medium) were supplemented with various combinations of Wnt3a- or ISEMF-conditioned medium (CM) and with glycogen synthase kinase 3 inhibitor (GSK3i), and their effects were ..
  22. Li D, Lin Z, Meng Q, Wang K, Wu J, Yan H. Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury. Eur J Pharmacol. 2017;809:13-19 pubmed publisher
    ..Furthermore, treatment of SCI rats with CBD enhanced mRNA expression of wnt3a, Lrp5 and ctnnb1 in femurs...
  23. Flanagan D, Barker N, Nowell C, Clevers H, Ernst M, Phesse T, et al. Loss of the Wnt receptor frizzled 7 in the mouse gastric epithelium is deleterious and triggers rapid repopulation in vivo. Dis Model Mech. 2017;10:971-980 pubmed publisher
    ..b>Wnt3a is required in the culture medium of gastric organoids, suggesting it is also important for the homeostasis of the ..
  24. Xu M, Bi C, Liu E, Dong T, Tsim K. Wnt3a induces the expression of acetylcholinesterase during osteoblast differentiation via the Runx2 transcription factor. J Biol Chem. 2017;292:12667-12678 pubmed publisher
    ..that AChE activity in osteoblast could be triggered by Wnt/?-catenin signaling, we added recombinant human Wnt3a to cultured osteoblasts and found that this addition induced expression of the ACHE gene and protein ..
  25. Paclíková P, Bernatik O, Radaszkiewicz T, Bryja V. N-terminal part of Dishevelled DEP domain is required for Wnt/?-catenin signaling in mammalian cells. Mol Cell Biol. 2017;: pubmed publisher
    ..In this study, we took advantage of DVL KO (DVL1/DVL2/DVL3-triple knockout) cells, fully deficient in Wnt3a-induced signaling events, and performed series of rescue experiments...
  26. Rieger M, Sims A, Coats E, Clarke R, Briegel K. The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers. Mol Cell Biol. 2010;30:4267-79 pubmed publisher
    ..factor (LEF) binding sites in the LBH locus and rapid beta-catenin-dependent upregulation of endogenous LBH by Wnt3a. In contrast, LBH induction by Wnt/beta-catenin signaling is inhibited by Wnt7a, which in limb development signals ..
  27. Shi Y, Yuan S, Li B, Wang J, Carlton S, Chung K, et al. Regulation of Wnt signaling by nociceptive input in animal models. Mol Pain. 2012;8:47 pubmed publisher
    ..In addition, Wnt3a, a prototypic Wnt ligand that activates the canonical pathway, is also enriched in the superficial layers...
  28. Tan C, Hirokawa Y, Burgess A. Analysis of Wnt signalling dynamics during colon crypt development in 3D culture. Sci Rep. 2015;5:11036 pubmed publisher
    ..and E-cadherin, spatial-temporal dynamics of these proteins in normal colon crypt cells stimulated with Wnt3A or inhibited by cycloheximide has been measured...
  29. Sakisaka Y, Tsuchiya M, Nakamura T, Tamura M, Shimauchi H, Nemoto E. Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells. Exp Cell Res. 2015;336:85-93 pubmed publisher
    ..Silencing expression of the Wnt5a gene in a dental follicle cell line resulted in enhancement of the Wnt3a (a representative canonical Wnt ligand)-mediated increase in alkaline phosphatase (ALP) expression...
  30. Bandín S, Morona R, González A. Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis. Front Neuroanat. 2015;9:107 pubmed publisher
    ..Common molecular features were observed in the thalamic prepatterning among vertebrates in which Wnt3a, Fez, Pax6 and Xiro1 expression were of particular importance in Xenopus...
  31. Cho Y, Bae H, Lee D, Yoon W, Woo K, Baek J, et al. Epigenetic Priming Confers Direct Cell Trans-Differentiation From Adipocyte to Osteoblast in a Transgene-Free State. J Cell Physiol. 2016;231:1484-94 pubmed publisher
    ..by modifying the epigenetic landscape with a DNA methyltransferase inhibitor (DNMTi), 5'-aza-dC, followed by Wnt3a treatment to signal osteogenesis...
  32. Gong J, Belinsky G, Sagheer U, Zhang X, Grippo P, Chung C. Pigment Epithelium-derived Factor (PEDF) Blocks Wnt3a Protein-induced Autophagy in Pancreatic Intraepithelial Neoplasms. J Biol Chem. 2016;291:22074-22085 pubmed
    ..In this study, canonical Wnt3a ligand induced autophagy markers and vacuoles in murine PanIN cells...
  33. Sun Y, Yuan M, Shi X, Liu M, Hu W. [Study on the influence of Wnt3a on osteogenetic differentiation ability of dental pulp stem cells induced by mineralizing medium]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2016;51:634-639 pubmed publisher
    b>Objective: To investigate the effects of Wnt3a protein on proliferation and osteogenic differentiation of human dental pulp stem cells(DPSC)...
  34. Yiew N, Chatterjee T, Tang Y, Pellenberg R, Stansfield B, Bagi Z, et al. A novel role for the Wnt inhibitor APCDD1 in adipocyte differentiation: Implications for diet-induced obesity. J Biol Chem. 2017;292:6312-6324 pubmed publisher
    ..silencing of APCDD1 in 3T3-L1 preadipocytes markedly increased the expression of Wnt signaling proteins (Wnt3a, Wnt5a, Wnt10b, LRP5, and β-catenin) and inhibited the expression of adipocyte differentiation markers (CCAAT/..
  35. Saito Y, Nakaoka T, Muramatsu T, Ojima H, Sukeda A, Sugiyama Y, et al. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system. Sci Rep. 2018;8:2821 pubmed publisher
    ..Studies using a mouse model of IHCC indicate that Wnt3a derived from macrophages recruited upon inflammation in the liver may promote the malignant transformation of ..
  36. Janeczek A, Tare R, Scarpa E, Moreno Jiménez I, Rowland C, Jenner D, et al. Transient Canonical Wnt Stimulation Enriches Human Bone Marrow Mononuclear Cell Isolates for Osteoprogenitors. Stem Cells. 2016;34:418-30 pubmed publisher
    ..Bone marrow mononuclear cells (BMMNCs) were isolated from patients undergoing hip arthroplasty and exposed to Wnt3A protein...
  37. Possidonio A, Soares C, Fontenele M, Morris E, Mouly V, Costa M, et al. Knockdown of Lmo7 inhibits chick myogenesis. FEBS Lett. 2016;590:317-29 pubmed publisher
    ..Both Wnt3a enriched medium and Bio, activators of the Wnt/beta-catenin pathway, could rescue the effects of the Lmo7 ..
  38. Hulin J, Nguyen T, Cui S, Marri S, Yu R, Downes M, et al. Barx2 and Pax7 Regulate Axin2 Expression in Myoblasts by Interaction with ?-Catenin and Chromatin Remodelling. Stem Cells. 2016;34:2169-82 pubmed publisher
    ..b>Wnt3a not only induces Barx2 mRNA, but also stabilises Barx2 protein in myoblasts; conversely, Wnt3a potently inhibits ..
  39. Grol M, Brooks P, Pereverzev A, Dixon S. P2X7 nucleotide receptor signaling potentiates the Wnt/?-catenin pathway in cells of the osteoblast lineage. Purinergic Signal. 2016;12:509-20 pubmed publisher
    ..Using MC3T3-E1 cells, we found that combined treatment with Wnt3a and the P2X7 agonist 2'(3')-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (BzATP) elicited more sustained ?-..
  40. Bandara N, Gurusinghe S, Lim S, Chen H, Chen S, Wang D, et al. Molecular control of nitric oxide synthesis through eNOS and caveolin-1 interaction regulates osteogenic differentiation of adipose-derived stem cells by modulation of Wnt/?-catenin signaling. Stem Cell Res Ther. 2016;7:182 pubmed
    ..Canonical Wnt signaling pathway-associated Wnt3a and Wnt8a gene expressions were increased in eNOS-CAV-1F92A cells undergoing osteogenesis whilst non-..
  41. Xavier S, Sahu R, Landes S, Yu J, Taylor R, Ayyadevara S, et al. Pericytes and immune cells contribute to complement activation in tubulointerstitial fibrosis. Am J Physiol Renal Physiol. 2017;312:F516-F532 pubmed publisher
    ..with increased production of proinflammatory cytokines, extracellular matrix components, collagens, and increased Wnt3a-mediated activation of Wnt/β-catenin signaling, which are hallmarks of myofibroblast activation...
  42. Merino Azpitarte M, Lozano E, Perugorria M, Esparza Baquer A, Erice O, Santos Laso Ã, et al. SOX17 regulates cholangiocyte differentiation and acts as a tumor suppressor in cholangiocarcinoma. J Hepatol. 2017;67:72-83 pubmed publisher
    ..In NHC, Wnt3a decreased SOX17 expression in a DNMT-dependent manner, whereas in CCA, DNMT1 inhibition or silencing upregulated ..
  43. Jing D, Yan Z, Cai J, Tong S, Li X, Guo Z, et al. Low-1 level mechanical vibration improves bone microstructure, tissue mechanical properties and porous titanium implant osseointegration by promoting anabolic response in type 1 diabetic rabbits. Bone. 2018;106:11-21 pubmed publisher
    ..WBV mitigated the reductions in femoral BMP2, OCN, Wnt3a, Lrp6, and ?-catenin and inhibited Sost mRNA expression but did not alter RANKL or RANK gene expression in T1DM ..
  44. Lee S, Oh H, Kim M, Kim G, Choi Y, Jo Y, et al. Effect of co-culture canine cumulus and oviduct cells with porcine oocytes during maturation and subsequent embryo development of parthenotes in vitro. Theriogenology. 2018;106:108-116 pubmed publisher
    ..Moreover, the levels of GDF9, prostaglandin-endoperoxide synthase 2 (PTGS2), WNT3A and matrix metalloproteinase 2 (MMP2) were significantly up-regulated in blastocysts from the cOC group...
  45. Saitoh T, Hirai M, Katoh M. Molecular cloning and characterization of WNT3A and WNT14 clustered in human chromosome 1q42 region. Biochem Biophys Res Commun. 2001;284:1168-75 pubmed
    Human WNT3A and WNT14 cDNAs were cloned and characterized. WNT3A and WNT14 encoded WNT family protein of 352 and 365 amino acids, respectively. The 3.0-kb WNT3A mRNA was moderately expressed in placenta, and the 4...
  46. Zhou Y, Sztukowska M, Wang Q, Inaba H, Potempa J, Scott D, et al. Noncanonical activation of β-catenin by Porphyromonas gingivalis. Infect Immun. 2015;83:3195-203 pubmed publisher
    ..gingivalis increased the activity of the β-catenin-dependent TCF/LEF promoter. P. gingivalis did not increase Wnt3a mRNA levels, a finding consistent with P...
  47. Szwed M, Kania K, Jóźwiak Z. Toxicity of doxorubicin-transferrin conjugate is connected to the modulation of Wnt/β-catenin pathway in human leukemia cells. Leuk Res. 2015;39:1096-102 pubmed publisher
    ..demonstrate that DOX-TRF conjugate effectively reduces transcription of key genes involved in β-catenin signaling transduction trial (Wnt3a, DVL-1, FZD-3, LRP5, β-catenin, DKK2) and triggers morphology alternations of CML cells.
  48. Narcisi R, Arikan O, Lehmann J, ten Berge D, van Osch G. Differential Effects of Small Molecule WNT Agonists on the Multilineage Differentiation Capacity of Human Mesenchymal Stem Cells. Tissue Eng Part A. 2016;22:1264-1273 pubmed
    ..Recently we showed that WNT3A protein promoted MSC proliferation and enhanced their chondrogenic potential, while simultaneously suppressing the ..
  49. Ruan W, Xue Y, Zong Y, Sun C. Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Mol Med Rep. 2016;14:4328-4334 pubmed publisher
    ..study, third?generation autologous?inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed and integrated into the genome of MC3T3?E1 murine mesenchymal stem cells (..
  50. Fuster Matanzo A, Manferrari G, Marchetti B, Pluchino S. Wnt3a promotes pro-angiogenic features in macrophages in vitro: Implications for stroke pathology. Exp Biol Med (Maywood). 2017;:1535370217746392 pubmed publisher
    b>Wnt3a is implicated in several key cellular processes and its expression has been reported in different cell types...
  51. Berwick D, Harvey K. The importance of Wnt signalling for neurodegeneration in Parkinson's disease. Biochem Soc Trans. 2012;40:1123-8 pubmed
    ..This suggests the prospect of targeting Wnt signalling pathways to modify PD progression. ..
  52. Le L, Swingler T, Crowe N, Vincent T, Barter M, Donell S, et al. The microRNA-29 family in cartilage homeostasis and osteoarthritis. J Mol Med (Berl). 2016;94:583-96 pubmed publisher
    ..The miR-29 family is regulated by TGF-β1 and IL-1 in chondrocytes. The miR-29 family negatively regulates Smad, NFκB, and canonical Wnt signalling. Several Wnt-related genes are direct targets of the miR-29 family. ..
  53. Benham Pyle B, Sim J, Hart K, PRUITT B, NELSON W. Increasing ?-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis. elife. 2016;5: pubmed publisher
    ..Activating ?-catenin through Casein Kinase I inhibition or Wnt3A addition increased ?-catenin-mediated transcription and strain-induced accumulation of cells in S/G2...
  54. Hübner K, Grassme K, Rao J, Wenke N, Zimmer C, Korte L, et al. Wnt signaling positively regulates endothelial cell fate specification in the Fli1a-positive progenitor population via Lef1. Dev Biol. 2017;430:142-155 pubmed publisher
    ..Additionally, we show that mesodermal Wnt3a-mediated signaling via the transcription factor Lef1 positively regulates EC specification (defined by kdrl ..
  55. Feng Y, Ren J, Gui Y, Wei W, Shu B, Lu Q, et al. Wnt/?-Catenin-Promoted Macrophage Alternative Activation Contributes to Kidney Fibrosis. J Am Soc Nephrol. 2018;29:182-193 pubmed publisher
    ..Treatment of macrophages with Wnt3a exacerbated IL-4- or TGF?1-induced macrophage alternative (M2) polarization and the phosphorylation and ..
  56. Niell N, Larriba M, Ferrer Mayorga G, Sanchez Perez I, Cantero R, Real F, et al. The human PKP2/plakophilin-2 gene is induced by Wnt/?-catenin in normal and colon cancer-associated fibroblasts. Int J Cancer. 2017;: pubmed publisher
    ..Here we present the first transcriptomic study on the effect of a Wnt factor on human colonic myofibroblasts. Wnt3A regulates the expression of 1,136 genes, of which 662 are upregulated and 474 are downregulated in CCD-18Co cells...
  57. Naschberger A, Orry A, Lechner S, Bowler M, Nurizzo D, Novokmet M, et al. Structural Evidence for a Role of the Multi-functional Human Glycoprotein Afamin in Wnt Transport. Structure. 2017;: pubmed publisher
    ..binding pocket of afamin was modeled as palmitoleic acid, presenting the native O-acylation on serine 209 in human Wnt3a. The modeled complex between the experimental afamin structure and a Wnt3a homology model based on the XWnt8-Fz8-..
  58. Hocevar B, Mou F, Rennolds J, Morris S, Cooper J, Howe P. Regulation of the Wnt signaling pathway by disabled-2 (Dab2). EMBO J. 2003;22:3084-94 pubmed
    ..Based on these results, we propose that Dab2 functions as a negative regulator of canonical Wnt signaling by stabilizing the beta-catenin degradation complex, which may contribute to its proposed role as a tumor suppressor. ..
  59. Yang E, Tacchelly Benites O, Wang Z, Randall M, Tian A, Benchabane H, et al. Wnt pathway activation by ADP-ribosylation. Nat Commun. 2016;7:11430 pubmed publisher
  60. Sindhavajiva P, Sastravaha P, Arksornnukit M, Pavasant P. Intermittent compressive force induces human mandibular-derived osteoblast differentiation via WNT/?-catenin signaling. J Cell Biochem. 2017;: pubmed publisher
    ..In addition, OSX, ALP, and WNT3A mRNA expression and ?-catenin nuclear translocation increased when ICF was applied...
  61. Xu T, Pan L, Ge Y, Li P, Meng X, Huang C, et al. TMEM88 mediates inflammatory cytokines secretion by regulating JNK/P38 and canonical Wnt/?-catenin signaling pathway in LX-2 cells. Inflammopharmacology. 2017;: pubmed publisher
    ..could increase the expression levels of canonical Wnt/?-catenin accompanied with upregulated phosphorylation of wnt3a, wnt10b and ?-catenin protein levels in response to TNF-?...
  62. Korvala J, Löija M, Makitie O, Sochett E, Juppner H, Schnabel D, et al. Rare variations in WNT3A and DKK1 may predispose carriers to primary osteoporosis. Eur J Med Genet. 2012;55:515-9 pubmed publisher
    ..The genes studied included DKK1, DKK2, WNT3A, WNT10B, AXIN1, SOST, TPH1 and 5-HTR1B. Two rare variants in WNT3A (c.152A > G, p.K51R) and DKK1 (c...
  63. Kaur N, Chettiar S, Rathod S, Rath P, Muzumdar D, Shaikh M, et al. Wnt3a mediated activation of Wnt/?-catenin signaling promotes tumor progression in glioblastoma. Mol Cell Neurosci. 2013;54:44-57 pubmed publisher
    ..We demonstrate that Wnt ligands - Wnt1 and Wnt3a are expressed in a graded manner in these tumors as well as over-expressed in glioma stem cell-lines...
  64. Johnson R, Merkel A, Page J, Ruppender N, Guelcher S, Sterling J. Wnt signaling induces gene expression of factors associated with bone destruction in lung and breast cancer. Clin Exp Metastasis. 2014;31:945-59 pubmed publisher
    ..levels in MDA-MB-231 and RWGT2 cells in vitro and promoted tumor-induced bone destruction in vivo in a ?-catenin/Wnt3a-dependent mechanism...
  65. Xing Y, Ma X, Guo H, Deng F, Yang J, Li Y. Wnt5a Suppresses ?-catenin Signaling during Hair Follicle Regeneration. Int J Med Sci. 2016;13:603-10 pubmed publisher
    ..JB6 cell line and found that the inhibitory effect of Wnt5a on cell proliferation could be rescued by the addition of Wnt3a. Our data reveal that Wnt5a suppresses the activation of ?-catenin signaling during hair follicle regeneration.
  66. Lian W, Wu R, Lee M, Chen Y, Sun Y, Wu S, et al. Subchondral mesenchymal stem cells from osteoarthritic knees display high osteogenic differentiation capacity through microRNA-29a regulation of HDAC4. J Mol Med (Berl). 2017;95:1327-1340 pubmed publisher
    ..They also displayed higher expressions of embryonic stem cell marker OCT3/4 and osteogenic factors Wnt3a, ?-catenin, and microRNA-29a (miR-29a), concomitant with lower expressions of joint-deleterious factors HDAC4, TGF-..
  67. Gao Y, Wang H. Inositol pentakisphosphate mediates Wnt/beta-catenin signaling. J Biol Chem. 2007;282:26490-502 pubmed
    b>Wnt3a stimulates lymphoid enhancer factor/T-cell factor protein-sensitive transcription, i.e. the canonical pathway, in mouse F9 embryonal tetratocarcinoma cells expressing rat Frizzled-1...
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    ..In light of the direct role that the Wnt pathway plays in human bone diseases and malignancies, our findings may support the development of novel therapeutic approaches that target Wnt signaling through LRP6. ..
  69. Steele B, Harper M, Macaulay I, Morrell C, Perez Tamayo A, Foy M, et al. Canonical Wnt signaling negatively regulates platelet function. Proc Natl Acad Sci U S A. 2009;106:19836-41 pubmed publisher
    ..We demonstrate that the Wnt3a ligand inhibits platelet adhesion, activation, dense granule secretion, and aggregation...
  70. Lei T, Liang Z, Li F, Tang C, Xie K, Wang P, et al. Pulsed electromagnetic fields (PEMF) attenuate changes in vertebral bone mass, architecture and strength in ovariectomized mice. Bone. 2017;108:10-19 pubmed publisher
    ..Real-time PCR showed that the canonical Wnt signaling pathway of the lumbar vertebrae, including Wnt3a, LRP5 and ?-catenin were markedly up-regulated in OVX+PEMF group compared with OVX group...
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    ..Interestingly, two different growth factors, EGF and Wnt3a, elicited distinct elastic force profiles revealed by AFM at the plasma membrane during receptor-mediated ..
  72. Yang X, He H, Gao Q, He C. Pulsed electromagnetic field improves subchondral bone microstructure in knee osteoarthritis rats through a Wnt/?-catenin signaling-associated mechanism. Bioelectromagnetics. 2017;: pubmed publisher
    ..Real-time PCR analysis showed that PEMF promoted tibial subchondral bone's gene expressions of Wnt3a, ?-catenin, and OPG, but did not alter LRP5 and RANKL mRNA levels...
  73. Li X, PLACENCIO V, Iturregui J, Uwamariya C, Sharif Afshar A, Koyama T, et al. Prostate tumor progression is mediated by a paracrine TGF-beta/Wnt3a signaling axis. Oncogene. 2008;27:7118-30 pubmed publisher
    ..LNCaP cell proliferation and tumorigenesis was observed by Tgfbr2(fspKO) prostate stroma as a result of elevated Wnt3a expression. Neutralizing antibodies to Wnt3a reversed LNCaP tumorigenesis...
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    ..Our results suggest that Wnt signaling activation at the cell-surface receptor level can contribute to breast cancer tumorigenesis. ..
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    ..Upon pre-treatment with WNT-3a, this complex disassociates, concurrent with a reduction in RhoA-GTP. Together these data implicate WNT-3a as a novel upstream regulator of small GTPase activity in platelets. ..
  76. Zhang X, Hu D, Lu Y, Feng T, Huang J. [Overexpression of Wnt3a inhibited the apoptosis of mouse embryonic liver stem cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013;29:1277-80 pubmed
    To study the effect of Wnt3a on the apoptosis and the expressions of apoptosis-related proteins in mouse embryonic liver stem cells (ELSCs). The mouse ELSC14...
  77. Mihara E, Hirai H, Yamamoto H, Tamura Kawakami K, Matano M, Kikuchi A, et al. Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin. elife. 2016;5: pubmed publisher
    ..We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidentally discovered that Wnt3a co-purified with a glycoprotein afamin derived from the ..
  78. Li N, Fu H, Hewitt S, Dimitrov D, Ho M. Therapeutically targeting glypican-2 via single-domain antibody-based chimeric antigen receptors and immunotoxins in neuroblastoma. Proc Natl Acad Sci U S A. 2017;114:E6623-E6631 pubmed publisher
    ..that the single-domain antibodies can inhibit active ?-catenin signaling by disrupting the interaction of GPC2 and Wnt3a. To explore GPC2 as a potential target in neuroblastoma, we have developed two forms of antibody therapeutics, ..
  79. Rajan S, Puri S, Kumar D, Babu M, Shankar K, Varshney S, et al. Novel indole and triazole based hybrid molecules exhibit potent anti-adipogenic and antidyslipidemic activity by activating Wnt3a/?-catenin pathway. Eur J Med Chem. 2018;143:1345-1360 pubmed publisher
    ..The mechanistic study suggests that compound 13m exhibit anti-adipogenic property by activating Wnt3a/?-catenin pathway, a known suppressor of key adipogenic genes PPAR? and C/EBP?...
  80. Moore W, Kern J, Bhat R, Commons T, Fukayama S, Goljer I, et al. Modulation of Wnt signaling through inhibition of secreted frizzled-related protein I (sFRP-1) with N-substituted piperidinyl diphenylsulfonyl sulfonamides. J Med Chem. 2009;52:105-16 pubmed publisher
    ..Details of the structure-activity relationships and biological activity from the first structural class of this scaffold will be discussed. ..
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    ..The treatment of cells with Wnt3a for 6h up-regulated the expression (fold change > 1...
  82. Skalka N, Caspi M, Lahav Ariel L, Loh Y, Hirschberg K, Rosin Arbesfeld R. Carboxypeptidase E (CPE) inhibits the secretion and activity of Wnt3a. Oncogene. 2016;35:6416-6428 pubmed publisher
    ..Here, we show that CPE and the Wnt3a ligand are co-secreted from cells...
  83. Han Y, Liu T, Zou Y, Ji L, Li Y, Li J, et al. Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro. J Diabetes Res. 2017;2017:5649191 pubmed publisher
    ..Furthermore, Wnt3a can significantly promote self-renewal of Pro-INS?+?EML cells...
  84. Rahman S, Park C, Baek J, Ryoo H, Woo K. Fibrin-Enhanced Canonical Wnt Signaling Directs Plasminogen Expression in Cementoblasts. Int J Mol Sci. 2017;18: pubmed publisher
    ..The OCCM30-fibrin showed higher levels of Wnt3a expression, nuclear translocation of ?-catenin, and T-cell factor (TCF) optimal motif (TOP) reporter activity than ..
  85. Wang Z, Wan X, Sang G, Zhao J, Zhu Q, Wang D. miR-15a-5p suppresses endometrial cancer cell growth via Wnt/?-catenin signaling pathway by inhibiting WNT3A. Eur Rev Med Pharmacol Sci. 2017;21:4810-4818 pubmed
    ..The global incidence has increased in recent years. microRNAs (miRNAs) regulate numerous biological processes by binding to the 3'UTR of target mRNA to down-regulate protein synthesis...
  86. Qiang Y, Chen Y, Stephens O, Brown N, Chen B, Epstein J, et al. Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma. Blood. 2008;112:196-207 pubmed publisher
    ..We demonstrate here that MM cell-derived DKK1, a soluble inhibitor of canonical Wnt signaling, disrupted Wnt3a-regulated OPG and RANKL expression in osteoblasts...
  87. Hara K, Kageji T, Mizobuchi Y, Kitazato K, Okazaki T, Fujihara T, et al. Blocking of the interaction between Wnt proteins and their co-receptors contributes to the anti-tumor effects of adenovirus-mediated DKK3 in glioblastoma. Cancer Lett. 2015;356:496-505 pubmed publisher
    ..We first demonstrated the non-specific interaction of Wnt3a and Wnt5a with the receptors LRP6 and ROR2 and the up-regulation of the Wnt pathway in glioblastoma cells...
  88. Lin S, Jiang T, Ye L, Han Z, Liu Y, Liu C, et al. The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A. Oncotarget. 2016;7:86051-86063 pubmed publisher
    ..The expression pattern of WNT3A is consistent with BRG1 in colon cancer tissues and WNT3A expression was inhibited in BRG1 knockdown cells...
  89. Suzuki A, Ozono K, Kubota T, Kondou H, Tachikawa K, Michigami T. PTH/cAMP/PKA signaling facilitates canonical Wnt signaling via inactivation of glycogen synthase kinase-3beta in osteoblastic Saos-2 cells. J Cell Biochem. 2008;104:304-17 pubmed
    ..b>Wnt3a and forskolin synergistically increased the TCF-dependent transactivation...
  90. Cisternas P, Salazar P, Silva Alvarez C, Barros L, Inestrosa N. Activation of Wnt Signaling in Cortical Neurons Enhances Glucose Utilization through Glycolysis. J Biol Chem. 2016;291:25950-25964 pubmed
    ..In the present study, we found that acute treatment with the Wnt3a ligand induced a large increase in glucose uptake, without changes in the expression or localization of glucose ..
  91. Narendra Talabattula V, Morgan P, Frech M, Uhrmacher A, Herchenröder O, Pützer B, et al. Non-canonical pathway induced by Wnt3a regulates ?-catenin via Pyk2 in differentiating human neural progenitor cells. Biochem Biophys Res Commun. 2017;491:40-46 pubmed publisher
    ..In this study, we showed that Wnt3a triggers the Wnt/Ca2+ signaling pathway, indicated by an increase of cytosolic free calcium ([Ca2+<..
  92. Kelly J, Kleemann D, Rudiger S, Walker S. Effects of grade of oocyte-cumulus complex and the interactions between grades on the production of blastocysts in the cow, ewe and lamb. Reprod Domest Anim. 2007;42:577-82 pubmed
  93. Zhu A, Shen L, Xu L, Chen W, Huang Y. Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats. Biochem Biophys Res Commun. 2017;493:474-480 pubmed publisher
    ..We investigated whether Wnt3a and Wnt5a were involved in the development of CPTP, concerning their regulation of inflammatory responses in a ..
  94. Kang Y, Park H, Chung H, Min H, Park E, Lee M, et al. Wnt/?-catenin signaling mediates the antitumor activity of magnolol in colorectal cancer cells. Mol Pharmacol. 2012;82:168-77 pubmed publisher
    ..Magnolol also suppressed Wnt3a-induced ?-catenin translocation and subsequent target gene expression in human embryonic kidney 293 cells...
  95. Wang Y, Liao J, Tang S, Shu J, Zhang W. HIV-1 gp120 Upregulates Brain-Derived Neurotrophic Factor (BDNF) Expression in BV2 Cells via the Wnt/β-Catenin Signaling Pathway. J Mol Neurosci. 2017;62:199-208 pubmed publisher
    ..Furthermore, gp120-treated BV2 also accumulated Wnt3a and β-catenin, suggesting the activation of the Wnt/β-catenin pathway...
  96. Serysheva E, Berhane H, Grumolato L, Demir K, Balmer S, Bodak M, et al. Wnk kinases are positive regulators of canonical Wnt/?-catenin signalling. EMBO Rep. 2013;14:718-25 pubmed publisher
    ..Importantly, knockdown of human WNK1 and WNK2 also results in decreased Wnt signalling in mammalian cell culture, suggesting that Wnk kinases have a conserved function in ensuring peak levels of canonical Wnt signalling. ..