Gene Symbol: WNT3A
Description: Wnt family member 3A
Alias: protein Wnt-3a, wingless-type MMTV integration site family, member 3A
Species: human
Products:     WNT3A

Top Publications

  1. Tauriello D, Haegebarth A, Kuper I, Edelmann M, Henraat M, Canninga Van Dijk M, et al. Loss of the tumor suppressor CYLD enhances Wnt/beta-catenin signaling through K63-linked ubiquitination of Dvl. Mol Cell. 2010;37:607-19 pubmed publisher
    ..Together, our results argue that loss of CYLD instigates tumor growth in human cylindromatosis through a mechanism in which hyperubiquitination of polymerized Dvl drives enhancement of Wnt responses...
  2. Gazit A, Yaniv A, Bafico A, Pramila T, Igarashi M, Kitajewski J, et al. Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response. Oncogene. 1999;18:5959-66 pubmed
    ..All of these findings provide strong evidence that Hfz1 is a functional partner for certain Wnts in inducing TCF dependent transcription. ..
  3. Lu W, Yamamoto V, Ortega B, Baltimore D. Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth. Cell. 2004;119:97-108 pubmed
    ..Thus, Ryk appears to play a crucial role in Wnt-mediated signaling. ..
  4. Vijayaragavan K, Szabo E, Bosse M, Ramos Mejia V, Moon R, Bhatia M. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell. 2009;4:248-62 pubmed publisher
    ..We demonstrate that activation of canonical Wnt signaling by Wnt3a promotes proliferation of human embryonic stem cells (hESCs)--precursors already committed to the hematopoietic ..
  5. Nalesso G, Sherwood J, Bertrand J, Pap T, Ramachandran M, De Bari C, et al. WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways. J Cell Biol. 2011;193:551-64 pubmed publisher
    ..We propose a novel model in which a single WNT can simultaneously activate different pathways with distinct and independent outcomes and with reciprocal regulation. This offers an opportunity for selective pharmacological targeting...
  6. Liu J, Ding X, Tang J, Cao Y, Hu P, Zhou F, et al. Enhancement of canonical Wnt/?-catenin signaling activity by HCV core protein promotes cell growth of hepatocellular carcinoma cells. PLoS ONE. 2011;6:e27496 pubmed publisher
    ..HCV core protein significantly enhances Tcf-dependent transcriptional activity induced by Wnt3A in HCC cell lines...
  7. Leng L, Tan Y, Gong F, Hu L, Ouyang Q, Zhao Y, et al. Differentiation of primordial germ cells from induced pluripotent stem cells of primary ovarian insufficiency. Hum Reprod. 2015;30:737-48 pubmed publisher
    ..We induced subline differentiation into germ cells by adding Wnt3a (30 ng/ml) and bone morphogenetic protein 4 (100 ng/ml)...
  8. Abed E, Delalandre A, Lajeunesse D. Beneficial effect of resveratrol on phenotypic features and activity of osteoarthritic osteoblasts. Arthritis Res Ther. 2017;19:151 pubmed publisher
    ..In contrast, RSV increased the mineralization of OA Ob. Moreover, whereas Wnt3a stimulates the Wnt/?-catenin pathway in these cells, RSV further increased the response to Wnt3a...
  9. Cassuto J, Folestad A, Göthlin J, Malchau H, Kärrholm J. The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients. Bone. 2018;107:66-77 pubmed publisher
    ..In contrast, Wnt3a, Dkk-3, Dkk-4, sFRP-3 and Wif-1 did not differ from preoperative levels or healthy subjects during the course of ..

More Information

Publications135 found, 100 shown here

  1. Kang M, Das J, Gurunathan S, Park H, Song H, Park C, et al. The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study. Theranostics. 2017;7:4735-4752 pubmed publisher
    ..decreased the inner cell mass and trophectoderm cell numbers as well as mRNA expression of B3gnt5 and Wnt3a in developed blastocysts, which decreased the implantation and developmental rates of full-term offspring ..
  2. Foulke Abel J, In J, Yin J, Zachos N, Kovbasnjuk O, Estes M, et al. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology. 2016;150:638-649.e8 pubmed publisher
    ..Enteroids differentiated upon withdrawal of WNT3A, yielding decreased crypt markers and increased villus-like characteristics...
  3. Lei T, Liang Z, Li F, Tang C, Xie K, Wang P, et al. Pulsed electromagnetic fields (PEMF) attenuate changes in vertebral bone mass, architecture and strength in ovariectomized mice. Bone. 2018;108:10-19 pubmed publisher
    ..Real-time PCR showed that the canonical Wnt signaling pathway of the lumbar vertebrae, including Wnt3a, LRP5 and β-catenin were markedly up-regulated in OVX+PEMF group compared with OVX group...
  4. Tagliaferri C, Wittrant Y, Davicco M, Walrand S, Coxam V. Muscle and bone, two interconnected tissues. Ageing Res Rev. 2015;21:55-70 pubmed publisher
    ..Prostaglandin E2 (PGE2) and Wnt3a, which are secreted by osteocytes, osteocalcin (OCN) and IGF-1, which are produced by osteoblasts and sclerostin ..
  5. Bosch P, Fuller L, Sleeth C, Weiner J. Akirin2 is essential for the formation of the cerebral cortex. Neural Dev. 2016;11:21 pubmed
    ..All of the neurons that are generated in Akirin2 mutants also undergo apoptosis by E12. In situ hybridization for Wnt3a and Wnt-responsive genes suggest defective formation and/or function of the cortical hem in Akirin2 null mice...
  6. Cheng P, Chen Y, Cheng W, Lu P, Chen H, Chen B, et al. Wnt Signaling Regulates Blood Pressure by Downregulating a GSK-3β-Mediated Pathway to Enhance Insulin Signaling in the Central Nervous System. Diabetes. 2015;64:3413-24 pubmed publisher
    ..Pretreatment with the LDL receptor-related protein (LRP) antagonist Dickkopf-1 (DKK1) significantly attenuated the Wnt3a-induced depressor effect and nitric oxide production...
  7. Sun W, Wang Y, Yan Q, Lu R, Shi B. Effects of Er-Zhi-Wan on microarchitecture and regulation of Wnt/?-catenin signaling pathway in alveolar bone of ovariectomized rats. J Huazhong Univ Sci Technolog Med Sci. 2014;34:114-119 pubmed publisher
    ..acid phosphatase 5b (TRAP5b), as well as mandibular mRNA expression of Wnt/?-catenin signaling pathway molecules wnt3a, low-density lipoprotein receptor-related protein 5 (LRP5), ?-catenin and dickkopf homolog 1 (DKK1)...
  8. Zhao H, Huang Y, Tao J. ST1926 Attenuates Steroid-Induced Osteoporosis in Rats by Inhibiting Inflammation Response. J Cell Biochem. 2017;118:2072-2086 pubmed publisher
    ..Also, OPG, ALP, and Wnt3a/β-catenin down-regulation as well as inflammation up-regulation could be reversed by ST1926 administration ..
  9. Zhang Y, Zhang L, Fan X, Yang W, Yu B, Kou J, et al. Captopril attenuates TAC-induced heart failure via inhibiting Wnt3a/β-catenin and Jak2/Stat3 pathways. Biomed Pharmacother. 2019;113:108780 pubmed publisher
    ..decreased the expression of cleaved capase-3, Bax, phosphorylated Jak2 (p-Jak2), phosphorylated Stat3 (p-Stat3), Wnt3a and β-catenin proteins, as well as increased Bcl-2 expression...
  10. Park J, Kwon H, Sohn E, Kim K, Kim B, Jeong S, et al. Inhibition of Wnt/β-catenin signaling mediates ursolic acid-induced apoptosis in PC-3 prostate cancer cells. Pharmacol Rep. 2013;65:1366-74 pubmed
    ..Furthermore, the GSK3β inhibitor SB216763 or Wnt3a-conditioned medium (Wnt3a-CM) reversed the cleavages of caspase-3 and PARP induced by ursolic acid in PC-3 cells...
  11. Ajmone Cat M, D Urso M, di Blasio G, Brignone M, De Simone R, Minghetti L. Glycogen synthase kinase 3 is part of the molecular machinery regulating the adaptive response to LPS stimulation in microglial cells. Brain Behav Immun. 2016;55:225-235 pubmed publisher
    ..The effects of GSK3 inhibition were mimicked by Wnt3a, added exogenously, and reversed by Inhibitor of Wnt-Response-1-endo, a pharmacological disruptor of the canonical ..
  12. Jiang L, Yin M, Wei X, Liu J, Wang X, Niu C, et al. Bach1 Represses Wnt/β-Catenin Signaling and Angiogenesis. Circ Res. 2015;117:364-375 pubmed publisher
    ..Bach1 overexpression impaired angiogenesis in mice with hind-limb ischemia and inhibited Wnt3a-stimulated angiogenic response and the expression of Wnt/β-catenin target genes, such as interleukin-8 and ..
  13. Han D, Cao C, Su Y, Wang J, Sun J, Chen H, et al. Ginkgo biloba exocarp extracts inhibits angiogenesis and its effects on Wnt/?-catenin-VEGF signaling pathway in Lewis lung cancer. J Ethnopharmacol. 2016;192:406-412 pubmed publisher
    ..The expression levels of vascular en-dothelial growth factor (VEGF) and VEGFR2 mRNA or Wnt3a, ?-catenin, VEGF, VEGFR2 and p-Akt/Akt protein expression were respectively tested by Quantitative Reverse ..
  14. Freyer N, Knöspel F, Strahl N, Amini L, Schrade P, Bachmann S, et al. Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor. Biores Open Access. 2016;5:235-48 pubmed publisher
    ..Differentiation into definitive endoderm (DE) was induced by adding activin A, Wnt3a, and sodium butyrate to the culture medium...
  15. Liu B, Zhang R, Tao G, Lehwald N, Liu B, Koh Y, et al. Augmented Wnt signaling as a therapeutic tool to prevent ischemia/reperfusion injury in liver: Preclinical studies in a mouse model. Liver Transpl. 2015;21:1533-42 pubmed publisher
    ..AML12 cells treated by Wnt3a or the glycogen synthase kinase 3β inhibitor LiCl exposed to H-R demonstrated decreased reactive oxygen species ..
  16. Gerbal Chaloin S, Dumé A, Briolotti P, Klieber S, Raulet E, Duret C, et al. The WNT/β-catenin pathway is a transcriptional regulator of CYP2E1, CYP1A2, and aryl hydrocarbon receptor gene expression in primary human hepatocytes. Mol Pharmacol. 2014;86:624-34 pubmed publisher
    ..P450 expression and WNT/β-catenin pathway activity in PHHs by modulating its activity with recombinant mouse Wnt3a (the canonical activator), inhibitors of glycogen synthase kinase 3β, and small-interfering RNA to invalidate ..
  17. Sun Y, Kuek V, Qiu H, Tickner J, Chen L, Wang H, et al. The emerging role of NPNT in tissue injury repair and bone homeostasis. J Cell Physiol. 2018;233:1887-1894 pubmed publisher
    ..alpha (TNF-?), transforming growth factor beta (TGF-?), oncostatin M (OSM), bone morphogenic protein 2 (BMP2), Wnt3a, Vitamin D3 , and microRNA-378 (miR378)...
  18. Jing D, Luo E, Cai J, Tong S, Zhai M, Shen G, et al. Mechanical Vibration Mitigates the Decrease of Bone Quantity and Bone Quality of Leptin Receptor-Deficient Db/Db Mice by Promoting Bone Formation and Inhibiting Bone Resorption. J Bone Miner Res. 2016;31:1713-24 pubmed publisher
    ..tibial alkaline phosphatase (ALP), OCN, Runt-related transcription factor 2 (RUNX2), type I collagen (COL1), BMP2, Wnt3a, Lrp6, and ?-catenin mRNA expression, and prevented the increases of tibial sclerostin (SOST), RANK, RANKL, RANL/..
  19. Merino Azpitarte M, Lozano E, Perugorria M, Esparza Baquer A, Erice O, Santos Laso A, et al. SOX17 regulates cholangiocyte differentiation and acts as a tumor suppressor in cholangiocarcinoma. J Hepatol. 2017;67:72-83 pubmed publisher
    ..In NHC, Wnt3a decreased SOX17 expression in a DNMT-dependent manner, whereas in CCA, DNMT1 inhibition or silencing upregulated ..
  20. Wu J, Fan W, Ma L, Geng X. miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/β-catenin pathway. Drug Des Devel Ther. 2018;12:3439-3447 pubmed publisher
    ..In addition, miR-708-5p mimics significantly inhibit Wnt3a/β-catenin pathway activity both in transcription and protein level, which could be reversed by the addition of R-..
  21. Abed E, Bouvard B, Martineau X, Jouzeau J, Reboul P, Lajeunesse D. Elevated hepatocyte growth factor levels in osteoarthritis osteoblasts contribute to their altered response to bone morphogenetic protein-2 and reduced mineralization capacity. Bone. 2015;75:111-9 pubmed publisher
    ..the TOPflash TCF/lef luciferase reporter assay and western blot analysis of β-catenin levels in response to Wnt3a stimulation. Mineralization in response to BMP-2 was evaluated by alizarin red staining...
  22. Chen W, You J, Zheng Q, Zhu Y. Downregulation of lncRNA OGFRP1 inhibits hepatocellular carcinoma progression by AKT/mTOR and Wnt/?-catenin signaling pathways. Cancer Manag Res. 2018;10:1817-1826 pubmed publisher
    ..transition (EMT), was inactivated by lncRNA OGFRP1 downregulation, including decreased expression of Wnt3a, ?-catenin, N-cadherin and vimentin and increased expression of E-cadherin...
  23. Lavergne E, Hendaoui I, Coulouarn C, Ribault C, Leseur J, Eliat P, et al. Blocking Wnt signaling by SFRP-like molecules inhibits in vivo cell proliferation and tumor growth in cells carrying active ?-catenin. Oncogene. 2011;30:423-33 pubmed publisher
    ..One of such molecules is V3Nter, a soluble SFRP-like frizzled polypeptide that binds to Wnt3a and inhibits Wnt signaling and expression of the ?-catenin target genes cyclin D1 and c-myc...
  24. Lin H, Angeli M, Chung K, Ejimadu C, Rosa A, Lee T. sFRP2 activates Wnt/?-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling. Am J Physiol Cell Physiol. 2016;311:C710-C719 pubmed publisher
    ..activates CFs in part through canonical Wnt/?-catenin signaling, as evidenced by increased expression of Axin2 and Wnt3a, but not Wnt5a, as well as accumulation of nuclear ?-catenin...
  25. Baruah J, Hitzman R, Zhang J, Chaudhuri S, Mastej V, Wary K. The allosteric glycogen synthase kinase-3 inhibitor NP12 limits myocardial remodeling and promotes angiogenesis in an acute myocardial infarction model. J Biol Chem. 2017;292:20785-20798 pubmed publisher
    ..NP12 administration mediated proliferation of reparative cells in the AMI hearts. In a time-course analysis, Wnt3a and NP12 stabilized β-catenin and increased expression of both Nanog and VEGFR2...
  26. Nygren M, Døsen Dahl G, Stubberud H, Walchli S, Munthe E, Rian E. beta-catenin is involved in N-cadherin-dependent adhesion, but not in canonical Wnt signaling in E2A-PBX1-positive B acute lymphoblastic leukemia cells. Exp Hematol. 2009;37:225-33 pubmed publisher
    ..Instead, beta-catenin is involved in N-cadherin-dependent adherence junctions, suggesting for the first time that leukemia-stroma interactions may be mediated via an N-cadherin-dependent mechanism. ..
  27. Chien A, Moore E, Lonsdorf A, Kulikauskas R, Rothberg B, Berger A, et al. Activated Wnt/beta-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model. Proc Natl Acad Sci U S A. 2009;106:1193-8 pubmed publisher
    ..vivo is recapitulated in B16 murine melanoma cells and in human melanoma cell lines cultured in vitro with either WNT3A or small-molecule activators of beta-catenin signaling...
  28. Byun M, Hwang J, Kim A, Kim K, Hwang E, Yaffe M, et al. Canonical Wnt signalling activates TAZ through PP1A during osteogenic differentiation. Cell Death Differ. 2014;21:854-63 pubmed publisher
    ..In this report, we show that TAZ is regulated by canonical Wnt signalling during osteogenic differentiation. Wnt3a increases TAZ expression and an inhibitor of GSK3?, a downstream effector of Wnt signalling, induces TAZ...
  29. Li X, Chen C, Wang F, Huang W, Liang Z, Xiao Y, et al. KCTD1 suppresses canonical Wnt signaling pathway by enhancing ?-catenin degradation. PLoS ONE. 2014;9:e94343 pubmed publisher
    ..Finally, we found that Wnt pathway member APC and tumor suppressor p53 influence KCTD1-mediated downregulation of ?-catenin. These results suggest that KCTD1 functions as a novel inhibitor of Wnt signaling pathway. ..
  30. Chen Y, Huang Q, Zhou H, Wang Y, Hu X, Li T. Inhibition of canonical WNT/β-catenin signaling is involved in leflunomide (LEF)-mediated cytotoxic effects on renal carcinoma cells. Oncotarget. 2016;7:50401-50416 pubmed publisher
    ..However, LEF treatment also triggered cell autophagy and elevated the expression of WNT3a, which ameliorated its cytotoxic effects...
  31. Sakisaka Y, Kanaya S, Nakamura T, Tamura M, Shimauchi H, Nemoto E. p38 MAP kinase is required for Wnt3a-mediated osterix expression independently of Wnt-LRP5/6-GSK3? signaling axis in dental follicle cells. Biochem Biophys Res Commun. 2016;478:527-32 pubmed publisher
    b>Wnt3a is a secreted glycoprotein that activates the glycogen synthase kinase-3? (GSK3?)/?-catenin signaling pathway through low-density-lipoprotein receptor-related protein (LRP)5/6 co-receptors...
  32. Luo L, Luo G, Fang Q, Huang S, Chen H. [The regulatory role of Wnt signaling pathway in the proliferation and activation of memory T cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1595-1599 pubmed
    ..The expression levels of Wnt3a and Wnt5a were significantly higher in mDCs than in imDCs...
  33. Teofili L, Martini M, Nuzzolo E, Capodimonti S, Iachininoto M, Cocomazzi A, et al. Endothelial progenitor cell dysfunction in myelodysplastic syndromes: possible contribution of a defective vascular niche to myelodysplasia. Neoplasia. 2015;17:401-9 pubmed publisher
    ..The addition of Wnt3A reduced the expression of intercellular cell adhesion molecule-1 on MDS ECFCs and restored the defective ..
  34. Carthy J, Engström U, Heldin C, Moustakas A. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling. J Cell Biochem. 2016;117:938-45 pubmed publisher
    ..non-Wnt related signaling activities which were present in commercially available preparations of recombinant Wnt3a. Specifically, we found that treatment of cultured fibroblasts with recombinant Wnt3a induced immediate activation ..
  35. Shin H, Islam R, Yoon W, Lee T, Cho Y, Bae H, et al. Pin1-mediated Modification Prolongs the Nuclear Retention of β-Catenin in Wnt3a-induced Osteoblast Differentiation. J Biol Chem. 2016;291:5555-65 pubmed publisher
    ..These results indicate that Pin1 could be a critical target to modulate β-catenin-mediated osteogenesis. ..
  36. Mimura S, Suga M, Okada K, Kinehara M, Nikawa H, Furue M. Bone morphogenetic protein 4 promotes craniofacial neural crest induction from human pluripotent stem cells. Int J Dev Biol. 2016;60:21-8 pubmed publisher
    ..morphogenetic protein (BMP) 4 in defined serum-free culture conditions including fibroblast growth factor-2 and Wnt3a from day 8 after NC specification induced the expression of cranial NC markers, AP2alpha, MSX1, and DLX1, during ..
  37. Zheng H, Jia L, Liu C, Rong Z, Zhong L, Yang L, et al. TREM2 Promotes Microglial Survival by Activating Wnt/β-Catenin Pathway. J Neurosci. 2017;37:1772-1784 pubmed publisher
    ..More importantly, treatment with Wnt3a, LiCl, or TDZD-8, which activates the β-catenin-mediated Wnt signaling pathway, rescued microglia survival and ..
  38. MacDonald B, Hien A, Zhang X, Iranloye O, Virshup D, Waterman M, et al. Disulfide bond requirements for active Wnt ligands. J Biol Chem. 2014;289:18122-36 pubmed publisher
    ..We systematically analyzed cysteines and associated disulfide bonds in the prototypic Wnt3a. Our data show that mutation of any individual cysteine of Wnt3a results in covalent Wnt oligomers through ectopic ..
  39. Hunter D, Bardet C, Mouraret S, Liu B, Singh G, Sadoine J, et al. Wnt Acts as a Prosurvival Signal to Enhance Dentin Regeneration. J Bone Miner Res. 2015;30:1150-9 pubmed publisher
    ..We engineered a liposome-reconstituted form of WNT3A then tested whether this biomimetic compound could activate cells in the injured tooth pulp and stimulate dentin ..
  40. Xing Y, Chen X, Cao Y, Huang J, Xie X, Wei Y. Expression of Wnt and Notch signaling pathways in inflammatory bowel disease treated with mesenchymal stem cell transplantation: evaluation in a rat model. Stem Cell Res Ther. 2015;6:101 pubmed publisher
    ..39±0.54) and the normal group (1.62±0.25) (P <0.05). The Wnt3a mRNA was more highly expressed in IBD rats (2.92±0.94) and decreased in MSCT rats (0.17±0.63, P <0.05)...
  41. Khalil H, Lei N, Brinkley G, Scott A, Wang J, Kar U, et al. A novel culture system for adult porcine intestinal crypts. Cell Tissue Res. 2016;365:123-34 pubmed publisher
    ..epidermal growth factor, noggin, and R-spondin 1 (ENR medium) were supplemented with various combinations of Wnt3a- or ISEMF-conditioned medium (CM) and with glycogen synthase kinase 3 inhibitor (GSK3i), and their effects were ..
  42. Li D, Lin Z, Meng Q, Wang K, Wu J, Yan H. Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury. Eur J Pharmacol. 2017;809:13-19 pubmed publisher
    ..Furthermore, treatment of SCI rats with CBD enhanced mRNA expression of wnt3a, Lrp5 and ctnnb1 in femurs...
  43. Flanagan D, Barker N, Nowell C, Clevers H, Ernst M, Phesse T, et al. Loss of the Wnt receptor frizzled 7 in the mouse gastric epithelium is deleterious and triggers rapid repopulation in vivo. Dis Model Mech. 2017;10:971-980 pubmed publisher
    ..b>Wnt3a is required in the culture medium of gastric organoids, suggesting it is also important for the homeostasis of the ..
  44. Xu M, Bi C, Liu E, Dong T, Tsim K. Wnt3a induces the expression of acetylcholinesterase during osteoblast differentiation via the Runx2 transcription factor. J Biol Chem. 2017;292:12667-12678 pubmed publisher
    ..that AChE activity in osteoblast could be triggered by Wnt/?-catenin signaling, we added recombinant human Wnt3a to cultured osteoblasts and found that this addition induced expression of the ACHE gene and protein ..
  45. Paclíková P, Bernatik O, Radaszkiewicz T, Bryja V. The N-Terminal Part of the Dishevelled DEP Domain Is Required for Wnt/β-Catenin Signaling in Mammalian Cells. Mol Cell Biol. 2017;37: pubmed publisher
    ..In this study, we took advantage of DVL knockout (DVL1/DVL2/DVL3 triple knockout) cells fully deficient in Wnt3a-induced signaling events and performed a series of rescue experiments...
  46. Rieger M, Sims A, Coats E, Clarke R, Briegel K. The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers. Mol Cell Biol. 2010;30:4267-79 pubmed publisher
    ..factor (LEF) binding sites in the LBH locus and rapid beta-catenin-dependent upregulation of endogenous LBH by Wnt3a. In contrast, LBH induction by Wnt/beta-catenin signaling is inhibited by Wnt7a, which in limb development signals ..
  47. Shi Y, Yuan S, Li B, Wang J, Carlton S, Chung K, et al. Regulation of Wnt signaling by nociceptive input in animal models. Mol Pain. 2012;8:47 pubmed publisher
    ..In addition, Wnt3a, a prototypic Wnt ligand that activates the canonical pathway, is also enriched in the superficial layers...
  48. Tan C, Hirokawa Y, Burgess A. Analysis of Wnt signalling dynamics during colon crypt development in 3D culture. Sci Rep. 2015;5:11036 pubmed publisher
    ..and E-cadherin, spatial-temporal dynamics of these proteins in normal colon crypt cells stimulated with Wnt3A or inhibited by cycloheximide has been measured...
  49. Sakisaka Y, Tsuchiya M, Nakamura T, Tamura M, Shimauchi H, Nemoto E. Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells. Exp Cell Res. 2015;336:85-93 pubmed publisher
    ..Silencing expression of the Wnt5a gene in a dental follicle cell line resulted in enhancement of the Wnt3a (a representative canonical Wnt ligand)-mediated increase in alkaline phosphatase (ALP) expression...
  50. Bandín S, Morona R, González A. Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis. Front Neuroanat. 2015;9:107 pubmed publisher
    ..Common molecular features were observed in the thalamic prepatterning among vertebrates in which Wnt3a, Fez, Pax6 and Xiro1 expression were of particular importance in Xenopus...
  51. Cho Y, Bae H, Lee D, Yoon W, Woo K, Baek J, et al. Epigenetic Priming Confers Direct Cell Trans-Differentiation From Adipocyte to Osteoblast in a Transgene-Free State. J Cell Physiol. 2016;231:1484-94 pubmed publisher
    ..by modifying the epigenetic landscape with a DNA methyltransferase inhibitor (DNMTi), 5'-aza-dC, followed by Wnt3a treatment to signal osteogenesis...
  52. Gong J, Belinsky G, Sagheer U, Zhang X, Grippo P, Chung C. Pigment Epithelium-derived Factor (PEDF) Blocks Wnt3a Protein-induced Autophagy in Pancreatic Intraepithelial Neoplasms. J Biol Chem. 2016;291:22074-22085 pubmed
    ..In this study, canonical Wnt3a ligand induced autophagy markers and vacuoles in murine PanIN cells...
  53. Sun Y, Yuan M, Shi X, Liu M, Hu W. [Study on the influence of Wnt3a on osteogenetic differentiation ability of dental pulp stem cells induced by mineralizing medium]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2016;51:634-639 pubmed publisher
    b>Objective: To investigate the effects of Wnt3a protein on proliferation and osteogenic differentiation of human dental pulp stem cells(DPSC)...
  54. Yiew N, Chatterjee T, Tang Y, Pellenberg R, Stansfield B, Bagi Z, et al. A novel role for the Wnt inhibitor APCDD1 in adipocyte differentiation: Implications for diet-induced obesity. J Biol Chem. 2017;292:6312-6324 pubmed publisher
    ..silencing of APCDD1 in 3T3-L1 preadipocytes markedly increased the expression of Wnt signaling proteins (Wnt3a, Wnt5a, Wnt10b, LRP5, and β-catenin) and inhibited the expression of adipocyte differentiation markers (CCAAT/..
  55. Saito Y, Nakaoka T, Muramatsu T, Ojima H, Sukeda A, Sugiyama Y, et al. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system. Sci Rep. 2018;8:2821 pubmed publisher
    ..Studies using a mouse model of IHCC indicate that Wnt3a derived from macrophages recruited upon inflammation in the liver may promote the malignant transformation of ..
  56. Yao T, Yang L, Li P, Wu H, Xie H, Shen X, et al. Association of Wnt3A gene variants with non-syndromic cleft lip with or without cleft palate in Chinese population. Arch Oral Biol. 2011;56:73-8 pubmed publisher
    ..This study aimed to evaluate the association between Wnt3A gene polymorphisms and NSCLP in Chinese population from Northwest China...
  57. Qi L, Sun B, Liu Z, Cheng R, Li Y, Zhao X. Wnt3a expression is associated with epithelial-mesenchymal transition and promotes colon cancer progression. J Exp Clin Cancer Res. 2014;33:107 pubmed publisher
    ..However, the roles of Wnt3a, the representative canonical Wnt ligand, in EMT and colon cancer progression have not yet been fully explored...
  58. Janeczek A, Tare R, Scarpa E, Moreno Jiménez I, Rowland C, Jenner D, et al. Transient Canonical Wnt Stimulation Enriches Human Bone Marrow Mononuclear Cell Isolates for Osteoprogenitors. Stem Cells. 2016;34:418-30 pubmed publisher
    ..Bone marrow mononuclear cells (BMMNCs) were isolated from patients undergoing hip arthroplasty and exposed to Wnt3A protein...
  59. Possidonio A, Soares C, Fontenele M, Morris E, Mouly V, Costa M, et al. Knockdown of Lmo7 inhibits chick myogenesis. FEBS Lett. 2016;590:317-29 pubmed publisher
    ..Both Wnt3a enriched medium and Bio, activators of the Wnt/beta-catenin pathway, could rescue the effects of the Lmo7 ..
  60. Hulin J, Nguyen T, Cui S, Marri S, Yu R, Downes M, et al. Barx2 and Pax7 Regulate Axin2 Expression in Myoblasts by Interaction with ?-Catenin and Chromatin Remodelling. Stem Cells. 2016;34:2169-82 pubmed publisher
    ..b>Wnt3a not only induces Barx2 mRNA, but also stabilises Barx2 protein in myoblasts; conversely, Wnt3a potently inhibits ..
  61. Grol M, Brooks P, Pereverzev A, Dixon S. P2X7 nucleotide receptor signaling potentiates the Wnt/?-catenin pathway in cells of the osteoblast lineage. Purinergic Signal. 2016;12:509-20 pubmed publisher
    ..Using MC3T3-E1 cells, we found that combined treatment with Wnt3a and the P2X7 agonist 2'(3')-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (BzATP) elicited more sustained ?-..
  62. Bandara N, Gurusinghe S, Lim S, Chen H, Chen S, Wang D, et al. Molecular control of nitric oxide synthesis through eNOS and caveolin-1 interaction regulates osteogenic differentiation of adipose-derived stem cells by modulation of Wnt/?-catenin signaling. Stem Cell Res Ther. 2016;7:182 pubmed
    ..Canonical Wnt signaling pathway-associated Wnt3a and Wnt8a gene expressions were increased in eNOS-CAV-1F92A cells undergoing osteogenesis whilst non-..
  63. Xavier S, Sahu R, Landes S, Yu J, Taylor R, Ayyadevara S, et al. Pericytes and immune cells contribute to complement activation in tubulointerstitial fibrosis. Am J Physiol Renal Physiol. 2017;312:F516-F532 pubmed publisher
    ..with increased production of proinflammatory cytokines, extracellular matrix components, collagens, and increased Wnt3a-mediated activation of Wnt/?-catenin signaling, which are hallmarks of myofibroblast activation...
  64. Jing D, Yan Z, Cai J, Tong S, Li X, Guo Z, et al. Low-1 level mechanical vibration improves bone microstructure, tissue mechanical properties and porous titanium implant osseointegration by promoting anabolic response in type 1 diabetic rabbits. Bone. 2018;106:11-21 pubmed publisher
    ..WBV mitigated the reductions in femoral BMP2, OCN, Wnt3a, Lrp6, and ?-catenin and inhibited Sost mRNA expression but did not alter RANKL or RANK gene expression in T1DM ..
  65. Lee S, Oh H, Kim M, Kim G, Choi Y, Jo Y, et al. Effect of co-culture canine cumulus and oviduct cells with porcine oocytes during maturation and subsequent embryo development of parthenotes in vitro. Theriogenology. 2018;106:108-116 pubmed publisher
    ..Moreover, the levels of GDF9, prostaglandin-endoperoxide synthase 2 (PTGS2), WNT3A and matrix metalloproteinase 2 (MMP2) were significantly up-regulated in blastocysts from the cOC group...
  66. Saitoh T, Hirai M, Katoh M. Molecular cloning and characterization of WNT3A and WNT14 clustered in human chromosome 1q42 region. Biochem Biophys Res Commun. 2001;284:1168-75 pubmed
    Human WNT3A and WNT14 cDNAs were cloned and characterized. WNT3A and WNT14 encoded WNT family protein of 352 and 365 amino acids, respectively. The 3.0-kb WNT3A mRNA was moderately expressed in placenta, and the 4...
  67. Zhou Y, Sztukowska M, Wang Q, Inaba H, Potempa J, Scott D, et al. Noncanonical activation of β-catenin by Porphyromonas gingivalis. Infect Immun. 2015;83:3195-203 pubmed publisher
    ..gingivalis increased the activity of the β-catenin-dependent TCF/LEF promoter. P. gingivalis did not increase Wnt3a mRNA levels, a finding consistent with P...
  68. Szwed M, Kania K, Jóźwiak Z. Toxicity of doxorubicin-transferrin conjugate is connected to the modulation of Wnt/β-catenin pathway in human leukemia cells. Leuk Res. 2015;39:1096-102 pubmed publisher
    ..demonstrate that DOX-TRF conjugate effectively reduces transcription of key genes involved in β-catenin signaling transduction trial (Wnt3a, DVL-1, FZD-3, LRP5, β-catenin, DKK2) and triggers morphology alternations of CML cells.
  69. Narcisi R, Arikan O, Lehmann J, ten Berge D, van Osch G. Differential Effects of Small Molecule WNT Agonists on the Multilineage Differentiation Capacity of Human Mesenchymal Stem Cells. Tissue Eng Part A. 2016;22:1264-1273 pubmed
    ..Recently we showed that WNT3A protein promoted MSC proliferation and enhanced their chondrogenic potential, while simultaneously suppressing the ..
  70. Ruan W, Xue Y, Zong Y, Sun C. Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Mol Med Rep. 2016;14:4328-4334 pubmed publisher
    ..study, third?generation autologous?inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed and integrated into the genome of MC3T3?E1 murine mesenchymal stem cells (..
  71. Fuster Matanzo A, Manferrari G, Marchetti B, Pluchino S. Wnt3a promotes pro-angiogenic features in macrophages in vitro: Implications for stroke pathology. Exp Biol Med (Maywood). 2018;243:22-28 pubmed publisher
    b>Wnt3a is implicated in several key cellular processes and its expression has been reported in different cell types...
  72. Berwick D, Harvey K. The importance of Wnt signalling for neurodegeneration in Parkinson's disease. Biochem Soc Trans. 2012;40:1123-8 pubmed
    ..This suggests the prospect of targeting Wnt signalling pathways to modify PD progression. ..
  73. Le L, Swingler T, Crowe N, Vincent T, Barter M, Donell S, et al. The microRNA-29 family in cartilage homeostasis and osteoarthritis. J Mol Med (Berl). 2016;94:583-96 pubmed publisher
    ..The miR-29 family is regulated by TGF-β1 and IL-1 in chondrocytes. The miR-29 family negatively regulates Smad, NFκB, and canonical Wnt signalling. Several Wnt-related genes are direct targets of the miR-29 family. ..
  74. Benham Pyle B, Sim J, Hart K, PRUITT B, NELSON W. Increasing ?-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis. elife. 2016;5: pubmed publisher
    ..Activating ?-catenin through Casein Kinase I inhibition or Wnt3A addition increased ?-catenin-mediated transcription and strain-induced accumulation of cells in S/G2...
  75. Hübner K, Grassme K, Rao J, Wenke N, Zimmer C, Korte L, et al. Wnt signaling positively regulates endothelial cell fate specification in the Fli1a-positive progenitor population via Lef1. Dev Biol. 2017;430:142-155 pubmed publisher
    ..Additionally, we show that mesodermal Wnt3a-mediated signaling via the transcription factor Lef1 positively regulates EC specification (defined by kdrl ..
  76. Feng Y, Ren J, Gui Y, Wei W, Shu B, Lu Q, et al. Wnt/β-Catenin-Promoted Macrophage Alternative Activation Contributes to Kidney Fibrosis. J Am Soc Nephrol. 2018;29:182-193 pubmed publisher
    ..Treatment of macrophages with Wnt3a exacerbated IL-4- or TGFβ1-induced macrophage alternative (M2) polarization and the phosphorylation and ..
  77. Niell N, Larriba M, Ferrer Mayorga G, Sanchez Perez I, Cantero R, Real F, et al. The human PKP2/plakophilin-2 gene is induced by Wnt/?-catenin in normal and colon cancer-associated fibroblasts. Int J Cancer. 2018;142:792-804 pubmed publisher
    ..Here we present the first transcriptomic study on the effect of a Wnt factor on human colonic myofibroblasts. Wnt3A regulates the expression of 1,136 genes, of which 662 are upregulated and 474 are downregulated in CCD-18Co cells...
  78. Naschberger A, Orry A, Lechner S, Bowler M, Nurizzo D, Novokmet M, et al. Structural Evidence for a Role of the Multi-functional Human Glycoprotein Afamin in Wnt Transport. Structure. 2017;25:1907-1915.e5 pubmed publisher
    ..binding pocket of afamin was modeled as palmitoleic acid, presenting the native O-acylation on serine 209 in human Wnt3a. The modeled complex between the experimental afamin structure and a Wnt3a homology model based on the XWnt8-Fz8-..
  79. Hocevar B, Mou F, Rennolds J, Morris S, Cooper J, Howe P. Regulation of the Wnt signaling pathway by disabled-2 (Dab2). EMBO J. 2003;22:3084-94 pubmed
    ..Based on these results, we propose that Dab2 functions as a negative regulator of canonical Wnt signaling by stabilizing the beta-catenin degradation complex, which may contribute to its proposed role as a tumor suppressor. ..
  80. Yang E, Tacchelly Benites O, Wang Z, Randall M, Tian A, Benchabane H, et al. Wnt pathway activation by ADP-ribosylation. Nat Commun. 2016;7:11430 pubmed publisher
  81. Sindhavajiva P, Sastravaha P, Arksornnukit M, Pavasant P. Intermittent compressive force induces human mandibular-derived osteoblast differentiation via WNT/β-catenin signaling. J Cell Biochem. 2018;119:3474-3485 pubmed publisher
    ..In addition, OSX, ALP, and WNT3A mRNA expression and β-catenin nuclear translocation increased when ICF was applied...
  82. Xu T, Pan L, Ge Y, Li P, Meng X, Huang C, et al. TMEM88 mediates inflammatory cytokines secretion by regulating JNK/P38 and canonical Wnt/β-catenin signaling pathway in LX-2 cells. Inflammopharmacology. 2018;26:1339-1348 pubmed publisher
    ..could increase the expression levels of canonical Wnt/β-catenin accompanied with upregulated phosphorylation of wnt3a, wnt10b and β-catenin protein levels in response to TNF-α...
  83. Korvala J, Löija M, Makitie O, Sochett E, Juppner H, Schnabel D, et al. Rare variations in WNT3A and DKK1 may predispose carriers to primary osteoporosis. Eur J Med Genet. 2012;55:515-9 pubmed publisher
    ..The genes studied included DKK1, DKK2, WNT3A, WNT10B, AXIN1, SOST, TPH1 and 5-HTR1B. Two rare variants in WNT3A (c.152A > G, p.K51R) and DKK1 (c...
  84. Kaur N, Chettiar S, Rathod S, Rath P, Muzumdar D, Shaikh M, et al. Wnt3a mediated activation of Wnt/?-catenin signaling promotes tumor progression in glioblastoma. Mol Cell Neurosci. 2013;54:44-57 pubmed publisher
    ..We demonstrate that Wnt ligands - Wnt1 and Wnt3a are expressed in a graded manner in these tumors as well as over-expressed in glioma stem cell-lines...
  85. Johnson R, Merkel A, Page J, Ruppender N, Guelcher S, Sterling J. Wnt signaling induces gene expression of factors associated with bone destruction in lung and breast cancer. Clin Exp Metastasis. 2014;31:945-59 pubmed publisher
    ..in MDA-MB-231 and RWGT2 cells in vitro and promoted tumor-induced bone destruction in vivo in a β-catenin/Wnt3a-dependent mechanism...
  86. Xing Y, Ma X, Guo H, Deng F, Yang J, Li Y. Wnt5a Suppresses ?-catenin Signaling during Hair Follicle Regeneration. Int J Med Sci. 2016;13:603-10 pubmed publisher
    ..JB6 cell line and found that the inhibitory effect of Wnt5a on cell proliferation could be rescued by the addition of Wnt3a. Our data reveal that Wnt5a suppresses the activation of ?-catenin signaling during hair follicle regeneration.
  87. Lian W, Wu R, Lee M, Chen Y, Sun Y, Wu S, et al. Subchondral mesenchymal stem cells from osteoarthritic knees display high osteogenic differentiation capacity through microRNA-29a regulation of HDAC4. J Mol Med (Berl). 2017;95:1327-1340 pubmed publisher
    ..They also displayed higher expressions of embryonic stem cell marker OCT3/4 and osteogenic factors Wnt3a, ?-catenin, and microRNA-29a (miR-29a), concomitant with lower expressions of joint-deleterious factors HDAC4, TGF-..
  88. Gao Y, Wang H. Inositol pentakisphosphate mediates Wnt/beta-catenin signaling. J Biol Chem. 2007;282:26490-502 pubmed
    b>Wnt3a stimulates lymphoid enhancer factor/T-cell factor protein-sensitive transcription, i.e. the canonical pathway, in mouse F9 embryonal tetratocarcinoma cells expressing rat Frizzled-1...
  89. Khan Z, Vijayakumar S, de la Torre T, Rotolo S, Bafico A. Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor. Mol Cell Biol. 2007;27:7291-301 pubmed
    ..In light of the direct role that the Wnt pathway plays in human bone diseases and malignancies, our findings may support the development of novel therapeutic approaches that target Wnt signaling through LRP6. ..
  90. Steele B, Harper M, Macaulay I, Morrell C, Perez Tamayo A, Foy M, et al. Canonical Wnt signaling negatively regulates platelet function. Proc Natl Acad Sci U S A. 2009;106:19836-41 pubmed publisher
    ..We demonstrate that the Wnt3a ligand inhibits platelet adhesion, activation, dense granule secretion, and aggregation...
  91. Zhang X, Ren J, Wang J, Li S, Zou Q, Gao N. Receptor-mediated endocytosis generates nanomechanical force reflective of ligand identity and cellular property. J Cell Physiol. 2017;: pubmed publisher
    ..Interestingly, two different growth factors, EGF and Wnt3a, elicited distinct elastic force profiles revealed by AFM at the plasma membrane during receptor-mediated ..
  92. Yang X, He H, Gao Q, He C. Pulsed electromagnetic field improves subchondral bone microstructure in knee osteoarthritis rats through a Wnt/?-catenin signaling-associated mechanism. Bioelectromagnetics. 2018;39:89-97 pubmed publisher
    ..Real-time PCR analysis showed that PEMF promoted tibial subchondral bone's gene expressions of Wnt3a, ?-catenin, and OPG, but did not alter LRP5 and RANKL mRNA levels...