WDR5

Summary

Gene Symbol: WDR5
Description: WD repeat domain 5
Alias: BIG-3, CFAP89, SWD3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein, SWD3, Set1c WD40 repeat protein, homolog, cilia and flagella associated protein 89
Species: human
Products:     WDR5

Top Publications

  1. Patel A, Dharmarajan V, Vought V, Cosgrove M. On the mechanism of multiple lysine methylation by the human mixed lineage leukemia protein-1 (MLL1) core complex. J Biol Chem. 2009;284:24242-56 pubmed publisher
    ..biochemically reconstituted and characterized a five-component 200-kDa MLL1 core complex containing human MLL1, WDR5, RbBP5, Ash2L, and DPY-30...
  2. Ang Y, Tsai S, Lee D, Monk J, Su J, Ratnakumar K, et al. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell. 2011;145:183-97 pubmed publisher
    ..Here, we show that WD repeat domain 5 (Wdr5), a core member of the mammalian Trithorax (trxG) complex, positively correlates with the undifferentiated state ..
  3. Cai Y, Jin J, Swanson S, Cole M, Choi S, Florens L, et al. Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. J Biol Chem. 2010;285:4268-72 pubmed publisher
    ..Two of its subunits, WD repeat domain 5 (WDR5) and host cell factor 1 (HCF1), are shared with members of the MLL/SET family of histone H3 lysine 4 (H3K4) ..
  4. Lee J, Kim D, Lee S, Yang Q, Lee D, Lee S, et al. A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4. Proc Natl Acad Sci U S A. 2009;106:8513-8 pubmed publisher
    ..Importantly, this study identifies a specific H3K4 methytransferase complex, ASCOM, as a physiologically relevant coactivator for p53 and implicates ASCOM in the p53 tumor suppression pathway in vivo. ..
  5. Ruthenburg A, Wang W, Graybosch D, Li H, Allis C, Patel D, et al. Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex. Nat Struct Mol Biol. 2006;13:704-12 pubmed
    b>WDR5 is a core component of SET1-family complexes that achieve transcriptional activation via methylation of histone H3 on Nzeta of Lys4 (H3K4)...
  6. Wysocka J, Myers M, Laherty C, Eisenman R, Herr W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 2003;17:896-911 pubmed
    ..These results suggest that HCF-1 can broadly regulate transcription, both positively and negatively, through selective modulation of chromatin structure. ..
  7. Tang Z, Chen W, Shimada M, Nguyen U, Kim J, Sun X, et al. SET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53. Cell. 2013;154:297-310 pubmed publisher
    ..These results establish a mechanism in which SET1C and p300 act cooperatively, through direct interactions and coupled histone modifications, to facilitate the function of p53. ..
  8. Dharmarajan V, Lee J, Patel A, Skalnik D, Cosgrove M. Structural basis for WDR5 interaction (Win) motif recognition in human SET1 family histone methyltransferases. J Biol Chem. 2012;287:27275-89 pubmed publisher
    ..MLL1 associates with a subcomplex containing WDR5, RbBP5, Ash2L, and DPY-30 (WRAD), which together form the MLL1 core complex that is required for sequential mono- ..
  9. Wu M, Tsai Y, Yang M, Huang C, Chang S, Chang C, et al. Interplay between HDAC3 and WDR5 is essential for hypoxia-induced epithelial-mesenchymal transition. Mol Cell. 2011;43:811-22 pubmed publisher
    ..Under hypoxia, HDAC3 interacts with hypoxia-induced WDR5, recruits the histone methyltransferase (HMT) complex to increase histone H3 lysine 4 (H3K4)-specific HMT activity, ..

More Information

Publications83

  1. Wu M, Wang P, Lee J, Martin Brown S, Florens L, Washburn M, et al. Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS. Mol Cell Biol. 2008;28:7337-44 pubmed publisher
    ..Given our in vivo and in vitro observations, it appears that the human Set1 complex plays a more widespread role in H3K4 trimethylation than do the MLL complexes in mammalian cells. ..
  2. Patel A, Vought V, Dharmarajan V, Cosgrove M. A conserved arginine-containing motif crucial for the assembly and enzymatic activity of the mixed lineage leukemia protein-1 core complex. J Biol Chem. 2008;283:32162-75 pubmed publisher
    ..members are regulated by interaction with a conserved core group of proteins that include the WD repeat protein-5 (WDR5), retinoblastoma-binding protein-5 (RbBP5), and the absent small homeotic-2-like protein (Ash2L)...
  3. Lee J, Skalnik D. CpG-binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex. J Biol Chem. 2005;280:41725-31 pubmed
  4. Wang Y, Liu L, Zhong B, Liu T, Li Y, Yang Y, et al. WDR5 is essential for assembly of the VISA-associated signaling complex and virus-triggered IRF3 and NF-kappaB activation. Proc Natl Acad Sci U S A. 2010;107:815-20 pubmed publisher
    ..Using a biochemical purification approach, we identified the WD repeat protein WDR5 as a VISA-associated protein. WDR5 was recruited to VISA in a viral infection dependent manner...
  5. Gori F, Divieti P, Demay M. Cloning and characterization of a novel WD-40 repeat protein that dramatically accelerates osteoblastic differentiation. J Biol Chem. 2001;276:46515-22 pubmed
    ..In conclusion, we have identified a novel WD-40 protein, induced by BMP-2 treatment, that dramatically accelerates the program of osteoblastic differentiation in stably transfected MC3T3E1 cells. ..
  6. Han Z, Guo L, Wang H, Shen Y, Deng X, Chai J. Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5. Mol Cell. 2006;22:137-44 pubmed
    The WD40 repeat protein WDR5 specifically associates with the K4-methylated histone H3 in human cells...
  7. Song J, Kingston R. WDR5 interacts with mixed lineage leukemia (MLL) protein via the histone H3-binding pocket. J Biol Chem. 2008;283:35258-64 pubmed publisher
    b>WDR5 is a component of the mixed lineage leukemia (MLL) complex, which methylates lysine 4 of histone H3, and was identified as a methylated Lys-4 histone H3-binding protein...
  8. Avdic V, Zhang P, Lanouette S, Groulx A, Tremblay V, Brunzelle J, et al. Structural and biochemical insights into MLL1 core complex assembly. Structure. 2011;19:101-8 pubmed publisher
    ..methyltransferases whose enzymatic activity depends on their interaction with a three-subunit complex composed of WDR5, RbBP5, and Ash2L...
  9. Gan Q, Thiebaud P, Theze N, Jin L, Xu G, Grant P, et al. WD repeat-containing protein 5, a ubiquitously expressed histone methyltransferase adaptor protein, regulates smooth muscle cell-selective gene activation through interaction with pituitary homeobox 2. J Biol Chem. 2011;286:21853-64 pubmed publisher
    WD repeat-containing protein 5 (WDR5) is a common component of mammalian mixed lineage leukemia methyltransferase family members and is important for histone H3 lysine 4 methylation (H3K4me), which has been implicated in control of ..
  10. Wysocka J, Swigut T, Milne T, Dou Y, Zhang X, Burlingame A, et al. WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development. Cell. 2005;121:859-72 pubmed
    ..we show that a common component of MLL1, MLL2, and hSet1 H3 K4 methyltransferase complexes, the WD40-repeat protein WDR5, directly associates with histone H3 di- and trimethylated at K4 and with H3-K4-dimethylated nucleosomes...
  11. Patel A, Dharmarajan V, Cosgrove M. Structure of WDR5 bound to mixed lineage leukemia protein-1 peptide. J Biol Chem. 2008;283:32158-61 pubmed publisher
    ..lineage leukemia protein-1 (MLL1) catalyzes histone H3 lysine 4 methylation and is regulated by interaction with WDR5 (WD-repeat protein-5), RbBP5 (retinoblastoma-binding protein-5), and the Ash2L (absent, small, homeotic discs-2-..
  12. Dou Y, Milne T, Ruthenburg A, Lee S, Lee J, Verdine G, et al. Regulation of MLL1 H3K4 methyltransferase activity by its core components. Nat Struct Mol Biol. 2006;13:713-9 pubmed
    ..of several H3K4 methyltransferase complexes is the presence of three structural components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain...
  13. Kim J, Akiyama M, Park J, Lin M, Shimo A, Ueki T, et al. Activation of an estrogen/estrogen receptor signaling by BIG3 through its inhibitory effect on nuclear transport of PHB2/REA in breast cancer. Cancer Sci. 2009;100:1468-78 pubmed publisher
    ..Our results imply that BIG3 overexpression is one of the important mechanisms causing the activation of the estrogen/ERalpha signaling pathway in the hormone-related growth of breast cancer cells. ..
  14. Cao F, Chen Y, Cierpicki T, Liu Y, Basrur V, Lei M, et al. An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain. PLoS ONE. 2010;5:e14102 pubmed publisher
    ..A common feature of the mammalian MLL/SET1 complexes is the presence of three core components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain...
  15. Avdic V, Zhang P, Lanouette S, Voronova A, Skerjanc I, Couture J. Fine-tuning the stimulation of MLL1 methyltransferase activity by a histone H3-based peptide mimetic. FASEB J. 2011;25:960-7 pubmed publisher
    ..features of this family is the regulation of their methyltransferase activity by a tripartite complex composed of WDR5, RbBP5, and Ash2L...
  16. Lee J, Skalnik D. Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. Mol Cell Biol. 2008;28:609-18 pubmed
    ..These results suggest a model for how the mammalian RNAP II machinery is linked with histone H3-Lys4 histone methyltransferase complexes at transcriptionally active genes. ..
  17. Patel S, Kim D, Levitan I, Dressler G. The BRCT-domain containing protein PTIP links PAX2 to a histone H3, lysine 4 methyltransferase complex. Dev Cell. 2007;13:580-92 pubmed
    ..Embryonic lethal ptip-null mutants and conditional mutants both show reduced levels of methylated H3K4. Thus, PTIP bridges DNA-binding developmental regulators to histone methyltransferase-dependent epigenetic regulation. ..
  18. Hughes C, Rozenblatt Rosen O, Milne T, Copeland T, Levine S, Lee J, et al. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol Cell. 2004;13:587-97 pubmed
    ..These results suggest that menin activates the transcription of differentiation-regulating genes by covalent histone modification, and that this activity is related to tumor suppression by MEN1. ..
  19. Issaeva I, Zonis Y, Rozovskaia T, Orlovsky K, Croce C, Nakamura T, et al. Knockdown of ALR (MLL2) reveals ALR target genes and leads to alterations in cell adhesion and growth. Mol Cell Biol. 2007;27:1889-903 pubmed
    ..ALR appears to be involved in the regulation of adhesion-related cytoskeletal events, which might affect cell growth and survival. ..
  20. Lee S, Lee D, Dou Y, Lee J, Lee B, Kwak E, et al. Coactivator as a target gene specificity determinant for histone H3 lysine 4 methyltransferases. Proc Natl Acad Sci U S A. 2006;103:15392-7 pubmed
  21. Zhang P, Lee H, Brunzelle J, Couture J. The plasticity of WDR5 peptide-binding cleft enables the binding of the SET1 family of histone methyltransferases. Nucleic Acids Res. 2012;40:4237-46 pubmed publisher
    ..Recent reports have demonstrated that a three-subunit complex composed of WD-repeat protein-5 (WDR5), retinoblastoma-binding protein-5 (RbBP5) and absent, small, homeotic disks-2-like (ASH2L) stimulates the ..
  22. Yokoyama A, Wang Z, Wysocka J, Sanyal M, Aufiero D, Kitabayashi I, et al. Leukemia proto-oncoprotein MLL forms a SET1-like histone methyltransferase complex with menin to regulate Hox gene expression. Mol Cell Biol. 2004;24:5639-49 pubmed
    ..These studies link the menin tumor suppressor protein with the MLL histone methyltransferase machinery, with implications for Hox gene expression in development and leukemia pathogenesis. ..
  23. Schuetz A, Allali Hassani A, Martin F, Loppnau P, Vedadi M, Bochkarev A, et al. Structural basis for molecular recognition and presentation of histone H3 by WDR5. EMBO J. 2006;25:4245-52 pubmed
    ..The WD40 domain of WDR5 represents a new class of histone methyl-lysine recognition domains that is important for recruiting H3K4 ..
  24. Kim J, Banerjee T, Vinckevicius A, Luo Q, Parker J, Baker M, et al. A role for WDR5 in integrating threonine 11 phosphorylation to lysine 4 methylation on histone H3 during androgen signaling and in prostate cancer. Mol Cell. 2014;54:613-25 pubmed publisher
    ..However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P, and this interaction facilitates the recruitment of the ..
  25. Lee J, Tate C, You J, Skalnik D. Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex. J Biol Chem. 2007;282:13419-28 pubmed
    ..450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82...
  26. Garapaty S, Xu C, Trojer P, Mahajan M, Neubert T, Samuels H. Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation. J Biol Chem. 2009;284:7542-52 pubmed publisher
    ..components revealed factors involved in histone methylation and cell cycle control and include Ash2L, RbBP5, WDR5, HCF-1, DBC-1, and EMSY...
  27. Gasparini F, Skobo T, Benato F, Gioacchini G, Voskoboynik A, Carnevali O, et al. Characterization of Ambra1 in asexual cycle of a non-vertebrate chordate, the colonial tunicate Botryllus schlosseri, and phylogenetic analysis of the protein group in Bilateria. Mol Phylogenet Evol. 2016;95:46-57 pubmed publisher
    ..By means of similarity searches we identified Wdr5 and Katnb1 as proteins evolutionarily associated to Ambra1...
  28. Fan Z, Kong X, Xia J, Wu X, Li H, Xu H, et al. The arginine methyltransferase PRMT5 regulates CIITA-dependent MHC II transcription. Biochim Biophys Acta. 2016;1859:687-96 pubmed publisher
    ..CIITA interacted with and recruited PRMT5 to the MHC II promoter and mediated the synergy between PRMT5 and ASH2/WDR5 to activate MHC II transcription...
  29. Dai X, Guo W, Zhan C, Liu X, Bai Z, Yang Y. WDR5 Expression Is Prognostic of Breast Cancer Outcome. PLoS ONE. 2015;10:e0124964 pubmed publisher
    b>WDR5 is a core component of the human mixed lineage leukemia-2 complex, which plays central roles in ER positive tumour cells and is a major driver of androgen-dependent prostate cancer cell proliferation...
  30. Chung C, Sun Z, Mullokandov G, Bosch A, Qadeer Z, Cihan E, et al. Cbx8 Acts Non-canonically with Wdr5 to Promote Mammary Tumorigenesis. Cell Rep. 2016;16:472-486 pubmed publisher
    ..We find that Cbx8 associates with non-PRC1 complexes containing the H3K4 methyltransferase complex component WDR5, which together regulate Notch gene expression...
  31. Kawabe Y, Wang Y, McKinnell I, Bedford M, Rudnicki M. Carm1 regulates Pax7 transcriptional activity through MLL1/2 recruitment during asymmetric satellite stem cell divisions. Cell Stem Cell. 2012;11:333-45 pubmed publisher
    ..the C-terminal cleavage forms of the trithorax proteins MLL1/2 resulting in the recruitment of the ASH2L:MLL1/2:WDR5:RBBP5 histone H3K4 methyltransferase complex to regulatory enhancers and the proximal promoter of Myf5...
  32. Li D, Chen W, Xu X, Jiang F, Wang L, Xie Y, et al. Structure-based design and synthesis of small molecular inhibitors disturbing the interaction of MLL1-WDR5. Eur J Med Chem. 2016;118:1-8 pubmed publisher
    ..Targeting MLL1-WDR5 protein-protein interaction (PPI) to inhibit the activity of histone methyltransferase of MLL1 complex is a novel ..
  33. Gori F, Friedman L, Demay M. Wdr5, a novel WD repeat protein, regulates osteoblast and chondrocyte differentiation in vivo. J Musculoskelet Neuronal Interact. 2005;5:338-9 pubmed
  34. Patel A, Vought V, Swatkoski S, Viggiano S, Howard B, Dharmarajan V, et al. Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation. J Biol Chem. 2014;289:868-84 pubmed publisher
    ..also report that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5, suggesting a restraint for the architectural arrangement of subunits within the complex...
  35. Gordon J, Fischer Cartlidge E, Barton Burke M. The Big 3: An Updated Overview of Colorectal, Breast, and Prostate Cancers. Nurs Clin North Am. 2017;52:27-52 pubmed publisher
    ..Current clinical and treatment-related issues are discussed along with nursing care and implications for cancer care. Future directions for these 3 cancers are addressed also. ..
  36. Thompson B, Tremblay V, Lin G, Bochar D. CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes. Mol Cell Biol. 2008;28:3894-904 pubmed publisher
    ..We also report the first demonstration of chromatin remodeling activity for a member of the CHD6-9 family of proteins, suggesting that CHD8 functions in transcription through the ATP-dependent modulation of chromatin structure. ..
  37. Wallach T, Schellenberg K, Maier B, Kalathur R, Porras P, Wanker E, et al. Dynamic circadian protein-protein interaction networks predict temporal organization of cellular functions. PLoS Genet. 2013;9:e1003398 pubmed publisher
    ..Moreover, we predict that circadian PPIs dynamically connect many important cellular processes (signal transduction, cell cycle, etc.) contributing to temporal organization of cellular physiology in an unprecedented manner. ..
  38. Yoshimaru T, Komatsu M, Matsuo T, Chen Y, Murakami Y, Mizuguchi K, et al. Targeting BIG3-PHB2 interaction to overcome tamoxifen resistance in breast cancer cells. Nat Commun. 2013;4:2443 pubmed publisher
    ..These findings suggest inhibiting the interaction between BIG3 and PHB2 may be a new therapeutic strategy for the treatment of luminal-type breast cancer. ..
  39. Mahajan K, Malla P, Lawrence H, Chen Z, Kumar Sinha C, Malik R, et al. ACK1/TNK2 Regulates Histone H4 Tyr88-phosphorylation and AR Gene Expression in Castration-Resistant Prostate Cancer. Cancer Cell. 2017;31:790-803.e8 pubmed publisher
    ..The WDR5/MLL2 complex reads the H4-Y88-phosphorylation marks and deposits the transcriptionally activating H3K4-trimethyl ..
  40. Schilders K, Eenjes E, Edel G, de Munck A, van Kempen M, Demmers J, et al. Generation of a biotinylatable Sox2 mouse model to identify Sox2 complexes in vivo. Transgenic Res. 2018;27:75-85 pubmed publisher
    ..Two of these partners, Wdr5 and Tcf3, were validated to confirm their association in Sox2 complexes...
  41. Luan Y, Blazer L, Hu H, Hajian T, Zhang J, Wu H, et al. Design of a fluorescent ligand targeting the S-adenosylmethionine binding site of the histone methyltransferase MLL1. Org Biomol Chem. 2016;14:631-638 pubmed publisher
    ..of MLL1 methyltransferase activity requires the production of at least a trimeric complex of MLL1, RbBP5 and WDR5 to elicit robust activity...
  42. Sun Y, Bell J, Carter D, Gherardi S, Poulos R, Milazzo G, et al. WDR5 Supports an N-Myc Transcriptional Complex That Drives a Protumorigenic Gene Expression Signature in Neuroblastoma. Cancer Res. 2015;75:5143-54 pubmed publisher
    ..b>WDR5 is a histone H3K4 presenter that has been found to have an essential role in H3K4 trimethylation...
  43. Kim J, Yu J, Abdulkadir S, Chakravarti D. KAT8 Regulates Androgen Signaling in Prostate Cancer Cells. Mol Endocrinol. 2016;30:925-36 pubmed publisher
    ..H3 at threonine 11, with subsequent recruitment of tryptophan, aspartic acid (WD) repeat-containing protein 5 (WDR5) and the su(var)3-9, enhancer of zeste, trithorax/mixed-lineage leukemia (SET1/MLL) histone methyltransferase ..
  44. Couture J, Collazo E, Trievel R. Molecular recognition of histone H3 by the WD40 protein WDR5. Nat Struct Mol Biol. 2006;13:698-703 pubmed
    The WD40-repeat protein WDR5 is a conserved subunit of Trithorax (TRX) histone methyltransferase complexes. WDR5 has been reported to selectively bind dimethylated Lys4 (K4me2) in histone H3 to promote K4 trimethylation by TRX...
  45. Grebien F, Vedadi M, Getlik M, Giambruno R, Grover A, Avellino R, et al. Pharmacological targeting of the Wdr5-MLL interaction in C/EBPα N-terminal leukemia. Nat Chem Biol. 2015;11:571-578 pubmed publisher
    ..We show that C/EBPα p30, but not the normal p42 isoform, preferentially interacts with Wdr5, a key component of SET/MLL (SET-domain/mixed-lineage leukemia) histone-methyltransferase complexes...
  46. Ma D, George C, Nomburg J, Pfaller C, Cattaneo R, Samuel C. Upon Infection, Cellular WD Repeat-Containing Protein 5 (WDR5) Localizes to Cytoplasmic Inclusion Bodies and Enhances Measles Virus Replication. J Virol. 2018;92: pubmed publisher
    ..Using measles virus-infected HeLa cells, we found that the WD repeat-containing protein 5 (WDR5), a subunit of histone H3 lysine 4 methyltransferases, was selectively recruited to virus-induced inclusion bodies...
  47. Wang C, Li G, Wu Y, Xi J, Kang J. LincRNA1230 inhibits the differentiation of mouse ES cells towards neural progenitors. Sci China Life Sci. 2016;59:443-54 pubmed publisher
    ..The interaction of LincRNA1230 with Wdr5 blocked the localization of Wdr5 at the promoters of early neural genes, thereby inhibiting the enrichment of ..
  48. De Santa F, Totaro M, Prosperini E, Notarbartolo S, Testa G, Natoli G. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell. 2007;130:1083-94 pubmed
  49. Schneider G, Saur D. In Vivo RNAi Screening for Pancreatic Cancer Drivers: PILOTing the WDR5-MYC Axis. Trends Cancer. 2016;2:391-392 pubmed publisher
    ..In a forward genetic screen focused on epigenetic modifiers, a WDR5-Myc axis that regulates the DNA replication checkpoint was identified and exploited in vivo for therapeutic ..
  50. Shinsky S, Hu M, Vought V, Ng S, Bamshad M, Shendure J, et al. A non-active-site SET domain surface crucial for the interaction of MLL1 and the RbBP5/Ash2L heterodimer within MLL family core complexes. J Mol Biol. 2014;426:2283-99 pubmed publisher
    ..Dimethylation of H3K4 requires a sub-complex including WRAD (WDR5, RbBP5, Ash2L, and DPY-30), which binds to each SET1 family member forming a minimal core complex that is required ..
  51. Kim K, Choi J, Heo K, Kim H, Levens D, Kohno K, et al. Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription. J Biol Chem. 2008;283:9113-26 pubmed publisher
    ..Therefore, these results reveal a previously unrecognized function of H1 as a transcriptional repressor as well as the underlying mechanism involving specific sets of factors in this repression process. ..
  52. Nakagawa T, Xiong Y. X-linked mental retardation gene CUL4B targets ubiquitylation of H3K4 methyltransferase component WDR5 and regulates neuronal gene expression. Mol Cell. 2011;43:381-91 pubmed publisher
    ..Here, we show that CUL4B, but not its paralog, CUL4A, targets WDR5, a core subunit of histone H3 lysine 4 (H3K4) methyltransferase complexes, for ubiquitylation and degradation in ..
  53. Chen J, Luo Q, Yuan Y, Huang X, Cai W, Li C, et al. Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion. Mol Cell Biol. 2010;30:5621-35 pubmed publisher
    ..This work now links mechanistically Pygo2's role in histone modification to its enhancement of the Wnt-dependent transcriptional program and cancer stem-like cell expansion. ..
  54. Nayak A, Viale Bouroncle S, Morsczeck C, Muller S. The SUMO-specific isopeptidase SENP3 regulates MLL1/MLL2 methyltransferase complexes and controls osteogenic differentiation. Mol Cell. 2014;55:47-58 pubmed publisher
    ..SET1/MLL complexes are composed of a histone methyltransferase and the regulatory components WDR5, RbBP5, Ash2L, and DPY-30...
  55. Wang Y, Faiola F, Xu M, Pan S, Martinez E. Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. J Biol Chem. 2008;283:33808-15 pubmed publisher
    ..incorporate in addition to GCN5 or PCAF (GCN5/PCAF), other epigenetic coregulators (ADA2-A, ADA3, STAF36, and WDR5), cofactors of chromatin assembly/remodeling and DNA replication machineries (POLE3/CHRAC17 and POLE4), the stress- ..
  56. Carugo A, Genovese G, Seth S, Nezi L, Rose J, Bossi D, et al. In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer. Cell Rep. 2016;16:133-147 pubmed publisher
    ..Focusing on epigenetic regulators, we identified WDR5, a core member of the COMPASS histone H3 Lys4 (H3K4) MLL (1-4) methyltransferase complex, as a top tumor ..
  57. Shi L, Sun L, Li Q, Liang J, Yu W, Yi X, et al. Histone demethylase JMJD2B coordinates H3K4/H3K9 methylation and promotes hormonally responsive breast carcinogenesis. Proc Natl Acad Sci U S A. 2011;108:7541-6 pubmed publisher
  58. Li X, Wang S, Li Y, Deng C, Steiner L, Xiao H, et al. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood. 2011;118:1386-94 pubmed publisher
    ..Therefore, our data reveal a synergistic role of hSET1 and NURF in regulating the USF-bound barrier insulator to prevent erythroid genes from encroachment of heterochromatin. ..
  59. Sun T, He J, Liang Q, Ren L, Yan T, Yu T, et al. LncRNA GClnc1 Promotes Gastric Carcinogenesis and May Act as a Modular Scaffold of WDR5 and KAT2A Complexes to Specify the Histone Modification Pattern. Cancer Discov. 2016;6:784-801 pubmed publisher
    ..Mechanistically, GClnc1 bound WDR5 (a key component of histone methyltransferase complex) and KAT2A histone acetyltransferase, acted as a modular ..
  60. Schulz Y, Freese L, Mänz J, Zoll B, Völter C, Brockmann K, et al. CHARGE and Kabuki syndromes: a phenotypic and molecular link. Hum Mol Genet. 2014;23:4396-405 pubmed publisher
    ..yeast two hybrid assays, we could show that, like KMT2D, CHD7 interacts with members of the WAR complex, namely WDR5, ASH2L and RbBP5...
  61. Xu Z, He Y, Ju J, Rank G, Cerruti L, Ma C, et al. The role of WDR5 in silencing human fetal globin gene expression. Haematologica. 2012;97:1632-40 pubmed publisher
    ..The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human ..
  62. Jin S, Zhao H, Yi Y, Nakata Y, Kalota A, Gewirtz A. c-Myb binds MLL through menin in human leukemia cells and is an important driver of MLL-associated leukemogenesis. J Clin Invest. 2010;120:593-606 pubmed publisher
  63. Huang S, Li X, Yusufzai T, Qiu Y, Felsenfeld G. USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier. Mol Cell Biol. 2007;27:7991-8002 pubmed
    ..These results show that USF1 plays a direct role in maintaining the barrier, supporting a model in which the insulator works as a barrier by maintaining a local environment of active chromatin. ..
  64. Dias J, Van Nguyen N, Georgiev P, Gaub A, Brettschneider J, Cusack S, et al. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. 2014;28:929-42 pubmed publisher
    ..within the complex and show that KANSL1 acts as a scaffold protein interacting with four other subunits, including WDR5, which in turn binds KANSL2...
  65. Ali A, Veeranki S, Chinchole A, Tyagi S. MLL/WDR5 Complex Regulates Kif2A Localization to Ensure Chromosome Congression and Proper Spindle Assembly during Mitosis. Dev Cell. 2017;41:605-622.e7 pubmed publisher
    Mixed-lineage leukemia (MLL), along with multisubunit (WDR5, RbBP5, ASH2L, and DPY30) complex catalyzes the trimethylation of H3K4, leading to gene activation. Here, we characterize a chromatin-independent role for MLL during mitosis...
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    ..Here, using a proteomic approach we identify a protein complex, containing Wdr5, Hdac1, Hdac2 and Rere (named WHHERE), which regulates RA signaling and controls embryonic symmetry...
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    WD repeat domain 5 (WDR5) plays an important role in various biological functions through the epigenetic regulation of gene transcription; however, its role in bladder cancer remains largely unknown...
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    ..These data define an expanded role for H1 in regulating gene transcription and illustrate its dependence on the elongation competence of RNAPII. ..
  69. Hopkin A, Gordon W, Klein R, Espitia F, Daily K, Zeller M, et al. GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program. PLoS Genet. 2012;8:e1002829 pubmed publisher
    ..The differentiation associated transcription factor GRHL3/GET1 recruits the ubiquitously expressed Trithorax complex to a subset of differentiation genes. ..
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    ..These findings are the first to demonstrate that post-translational modifications occur on the Ash2L protein and provide a novel example of cross-talk between chromatin-modifying enzyme complexes. ..
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    ..A model is presented for how epigenetic cross talk may explain the finding of redundant functional domains within Cfp1. ..
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    ..Understanding this regulation of PHB2 nuclear import may provide therapeutic strategies for controlling E2/ERα signals in breast cancer cells. ..
  74. Ansari K, Mishra B, Mandal S. Human CpG binding protein interacts with MLL1, MLL2 and hSet1 and regulates Hox gene expression. Biochim Biophys Acta. 2008;1779:66-73 pubmed
    ..These results demonstrated that CGBP interacts with MLL1, MLL2 as well as hSet1 HMTs and plays critical roles in regulations of MLL target genes. ..