Gene Symbol: VPS54
Description: VPS54, GARP complex subunit
Alias: HCC8, PPP1R164, SLP-8p, VPS54L, hVps54L, vacuolar protein sorting-associated protein 54, hepatocellular carcinoma protein 8, protein phosphatase 1, regulatory subunit 164, tumor antigen HOM-HCC-8, tumor antigen SLP-8p, vacuolar protein sorting 54 homolog
Species: human
Products:     VPS54

Top Publications

  1. Liewen H, Meinhold Heerlein I, Oliveira V, Schwarzenbacher R, Luo G, Wadle A, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp Cell Res. 2005;306:24-34 pubmed
    ..Taken together, GARP shows significant conservation between various species but diversification and specialization result in important differences in human cells. ..
  2. Pérez Victoria F, Abascal Palacios G, Tascón I, Kajava A, Magadán J, Pioro E, et al. Structural basis for the wobbler mouse neurodegenerative disorder caused by mutation in the Vps54 subunit of the GARP complex. Proc Natl Acad Sci U S A. 2010;107:12860-5 pubmed publisher
    ..Mutation of leucine-967 to glutamine in the Vps54 subunit of GARP is responsible for spinal muscular atrophy in the wobbler mouse, an animal model of amyotrophic ..
  3. Pérez Victoria F, Mardones G, Bonifacino J. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol Biol Cell. 2008;19:2350-62 pubmed publisher
    ..Depletion of any of the three GARP subunits, Vps52, Vps53, or Vps54, by RNAi impairs sorting of the precursor of the acid hydrolase, cathepsin D, to lysosomes and leads to its ..
  4. Pérez Victoria F, Schindler C, Magadán J, Mardones G, Delevoye C, Romao M, et al. Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol Biol Cell. 2010;21:3386-95 pubmed publisher
    ..library yielded a phylogenetically conserved protein, Ang2/Fat-free, which interacts with human Vps52, Vps53 and Vps54. Human Ang2 is larger than yeast Vps51p, but exhibits significant homology in an N-terminal coiled-coil region ..
  5. Pérez Victoria F, Bonifacino J. Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network. Mol Cell Biol. 2009;29:5251-63 pubmed publisher
    ..These interactions depend on the N-terminal regions of Vps53 and Vps54 and the SNARE motif of the SNAREs...
  6. Conboy M, Cyert M. Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology. Mol Biol Cell. 2000;11:2429-43 pubmed
    We have characterized LUV1/RKI1/TCS3/VPS54, a novel yeast gene required to maintain normal vacuolar morphology. The luv1 mutant was identified in a genetic screen for mutants requiring the phosphatase calcineurin for vegetative growth...
  7. Bonifacino J, Hierro A. Transport according to GARP: receiving retrograde cargo at the trans-Golgi network. Trends Cell Biol. 2011;21:159-67 pubmed publisher
    ..X-ray crystallography of the Vps53 and Vps54 subunits of GARP has revealed that this complex is structurally related to other tethering factors such as the ..
  8. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..Mutation of VPS52, VPS53, or VPS54 results in the missorting of 70% of the vacuolar hydrolase carboxypeptidase Y as well as the mislocalization of ..
  9. Wei J, Zhang Y, Luo J, Wang J, Zhou Y, Miao H, et al. The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes. Cell Rep. 2017;19:2823-2835 pubmed publisher
    ..Further, Vps54 mutant mice displayed reduced cellular NPC2 protein levels and increased cholesterol accumulation, underscoring ..

More Information


  1. Gershlick D, Schindler C, Chen Y, Bonifacino J. TSSC1 is novel component of the endosomal retrieval machinery. Mol Biol Cell. 2016;27:2867-78 pubmed publisher
    ..Two tethering complexes named GARP (composed of ANG2, VPS52, VPS53, and VPS54 subunits) and EARP (composed of ANG2, VPS52, VPS53, and Syndetin subunits) were previously shown to participate in ..
  2. Hirata T, Fujita M, Nakamura S, Gotoh K, Motooka D, Murakami Y, et al. Post-Golgi anterograde transport requires GARP-dependent endosome-to-TGN retrograde transport. Mol Biol Cell. 2015;26:3071-84 pubmed publisher
    ..Knockout (KO) of each of the four GARP subunits, VPS51-VPS54, in HEK293 cells caused severely defective anterograde transport of both glycosylphosphatidylinositol (GPI)-..
  3. Fari K, Takács S, Ungar D, Sinka R. The role of acroblast formation during Drosophila spermatogenesis. Biol Open. 2016;5:1102-10 pubmed publisher
    Protein recycling is important for maintaining homeostasis of the Golgi and its cisternae. The Vps54 (Scat) protein, a subunit of the GARP tethering complex, is a central factor in retrograde transport to the trans-Golgi...
  4. Gioria M, Pasini M, Berruti G. Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant. Cell Tissue Res. 2017;369:413-427 pubmed publisher
    ..We also study wobbler mice, whose Vps54 mutation causes motor neuron degeneration and male infertility...
  5. Realegeno S, Puschnik A, Kumar A, Goldsmith C, Burgado J, Sambhara S, et al. Monkeypox Virus Host Factor Screen Using Haploid Cells Identifies Essential Role of GARP Complex in Extracellular Virus Formation. J Virol. 2017;91: pubmed publisher
    ..We validated the role of a set of vacuolar protein sorting (VPS) genes during infection, VPS51 to VPS54 (VPS51-54), which comprise the Golgi-associated retrograde protein (GARP) complex...
  6. Meisler M, Russ C, Montgomery K, Greenway M, Ennis S, Hardiman O, et al. Evaluation of the Golgi trafficking protein VPS54 (wobbler) as a candidate for ALS. Amyotroph Lateral Scler. 2008;9:141-8 pubmed publisher
    b>VPS54 is a component of the Golgi-associated retrograde protein (GARP) complex of vesicle sorting proteins...
  7. Schindler C, Chen Y, Pu J, Guo X, Bonifacino J. EARP is a multisubunit tethering complex involved in endocytic recycling. Nat Cell Biol. 2015;17:639-50 pubmed publisher
    ..share the Ang2, Vps52 and Vps53 subunits, but EARP contains an uncharacterized protein, syndetin, in place of the Vps54 subunit of GARP...
  8. Fuchs S, Resch K, Thiel C, Ulbrich M, Platzer M, Jockusch H, et al. Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14. BMC Genet. 2002;3:14 pubmed
    ..The possibility arises that there might be hitherto unknown effects of mutations, in addition to structural changes of the mRNA or regulatory abnormalities. ..
  9. Ejzykowicz D, Locken K, Ruiz F, Manandhar S, Olson D, Gharakhanian E. Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function. Curr Genet. 2017;63:531-551 pubmed publisher
    ..a super HHY (s-HHY) subgroup of genes comprised of ARF1, CHC1, DRS2, SAC1, VPS1, VPS34, VPS45, VPS52, and VPS54 that function exclusively or inclusively at trans-Golgi and late endosome interface...
  10. Walter L, Stark S, Helou K, Flügge P, Levan G, Günther E. Identification, characterization and cytogenetic mapping of a yeast Vps54 homolog in rat and mouse. Gene. 2002;285:213-20 pubmed
    A novel gene, VPS54-like (Vps54l), is described in the rat that is homologous to the yeast Vps54 gene which is known to be involved in intracellular protein sorting...
  11. Corrado L, Gagliardi S, Carlomagno Y, Mennini T, Ticozzi N, Mazzini L, et al. VPS54 genetic analysis in ALS Italian cohort. Eur J Neurol. 2011;18:e41-2 pubmed publisher
  12. Rosa Ferreira C, Christis C, Torres I, Munro S. The small G protein Arl5 contributes to endosome-to-Golgi traffic by aiding the recruitment of the GARP complex to the Golgi. Biol Open. 2015;4:474-81 pubmed publisher
    ..Thus it appears that Arl5 is one of the factors that directs the recruitment of the GARP complex to the trans-Golgi, and this function is conserved in both flies and humans. ..