Gene Symbol: VPS4B
Description: vacuolar protein sorting 4 homolog B
Alias: MIG1, SKD1, SKD1B, VPS4-2, vacuolar protein sorting-associated protein 4B, cell migration-inducing 1, cell migration-inducing gene 1 protein, suppressor of K(+) transport growth defect 1, suppressor of K+ transport defect 1, vacuolar protein sorting 4B
Species: human
Products:     VPS4B

Top Publications

  1. Périer F, Coulter K, Liang H, Radeke C, Gaber R, Vandenberg C. Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast. FEBS Lett. 1994;351:286-90 pubmed
    ..These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in ..
  2. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses. ..
  3. Hislop J, Marley A, von Zastrow M. Role of mammalian vacuolar protein-sorting proteins in endocytic trafficking of a non-ubiquitinated G protein-coupled receptor to lysosomes. J Biol Chem. 2004;279:22522-31 pubmed
    ..Agonist-induced proteolysis of DOR was significantly inhibited by dominant negative mutant versions of Vps4/Skd1, an AAA-family ATPase required for a late step in lysosomal sorting of ubiquitinated membrane cargo...
  4. Scott A, Gaspar J, Stuchell Brereton M, Alam S, Skalicky J, Sundquist W. Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A. Proc Natl Acad Sci U S A. 2005;102:13813-8 pubmed
  5. Scott A, Chung H, Gonciarz Swiatek M, Hill G, Whitby F, Gaspar J, et al. Structural and mechanistic studies of VPS4 proteins. EMBO J. 2005;24:3658-69 pubmed
    ..Here, we report the crystal structure of monomeric apo human VPS4B/SKD1 (hVPS4B), which is composed of five distinct elements: a poorly ordered N-terminal MIT domain that binds ..
  6. Stuchell Brereton M, Skalicky J, Kieffer C, Karren M, Ghaffarian S, Sundquist W. ESCRT-III recognition by VPS4 ATPases. Nature. 2007;449:740-4 pubmed
    ..Here we show that the microtubule interacting and transport (MIT) domains of human VPS4A and VPS4B bind conserved sequence motifs located at the carboxy termini of the CHMP1-3 class of ESCRT-III proteins...
  7. Lata S, Schoehn G, Jain A, Pires R, Piehler J, Gottlinger H, et al. Helical structures of ESCRT-III are disassembled by VPS4. Science. 2008;321:1354-7 pubmed publisher
    ..Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4. ..
  8. Baietti M, Zhang Z, Mortier E, Melchior A, Degeest G, Geeraerts A, et al. Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14:677-85 pubmed publisher
    ..This study identifies a key role for syndecan-syntenin-ALIX in membrane transport and signalling processes. ..
  9. Bishop N, Woodman P. ATPase-defective mammalian VPS4 localizes to aberrant endosomes and impairs cholesterol trafficking. Mol Biol Cell. 2000;11:227-39 pubmed
    ..The phenotype described here is largely consistent with the defects in vacuolar sorting associated with class E vps mutants in yeast, and a role for mammalian VPS4 is discussed in this context. ..

More Information

Publications103 found, 100 shown here

  1. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  2. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    ..These studies point to a role for direct binding between LIP5 and ESCRT-III proteins that is likely to complement LIP5's previously described ability to regulate VPS4 activity. ..
  3. Bajorek M, Morita E, Skalicky J, Morham S, Babst M, Sundquist W. Biochemical analyses of human IST1 and its function in cytokinesis. Mol Biol Cell. 2009;20:1360-73 pubmed publisher
    ..In contrast, IST1 depletion did not inhibit human immunodeficiency virus-1 budding. Thus, IST1 and CHMP1 act together to recruit and modulate specific VPS4 activities required during the final stages of cell division. ..
  4. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
    ..We found that depletion of VPS4A, VPS4B, or any of the 11 different human ESCRT-III (CHMP) proteins inhibited abscission...
  5. Skalicky J, Arii J, Wenzel D, Stubblefield W, Katsuyama A, Uter N, et al. Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport. J Biol Chem. 2012;287:43910-26 pubmed publisher
    ..Our studies thus reveal how the tandem MIT domain of LIP5 binds different types of ESCRT-III proteins, promoting assembly of active VPS4 enzymes on the polymeric ESCRT-III substrate. ..
  6. Pöhler R, Krahn J, van den Boom J, Dobrynin G, Kaschani F, Eggenweiler H, et al. A Non-Competitive Inhibitor of VCP/p97 and VPS4 Reveals Conserved Allosteric Circuits in Type I and II AAA ATPases. Angew Chem Int Ed Engl. 2018;57:1576-1580 pubmed publisher
    ..inhibitor of the type II AAA ATPase human ubiquitin-directed unfoldase (VCP)/p97 and the type I AAA ATPase VPS4B. Subsequent proteomic, genetic and biochemical studies indicate that MSC1094308 binds to a previously ..
  7. Zhang L, Ge Y, Fuchs E. miR-125b can enhance skin tumor initiation and promote malignant progression by repressing differentiation and prolonging cell survival. Genes Dev. 2014;28:2532-46 pubmed publisher
    ..On the other hand, it indirectly prolongs activated (phosphorylated) EGFR signaling by repressing Vps4b (vacuolar protein-sorting 4 homolog B), encoding a protein implicated in negatively regulating the endosomal ..
  8. Yao Y, Tsuchiyama S, Yang C, Bulteau A, He C, Robison B, et al. Proteasomes, Sir2, and Hxk2 form an interconnected aging network that impinges on the AMPK/Snf1-regulated transcriptional repressor Mig1. PLoS Genet. 2015;11:e1004968 pubmed publisher
    ..premature induction of respiration in cells with increased proteasome activity originates from enhanced turnover of Mig1, an AMPK/Snf1 regulated transcriptional repressor that prevents the induction of genes required for respiration...
  9. Wollman A, Leake M. Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time. Faraday Discuss. 2015;184:401-24 pubmed publisher
    ..It uses the multi-copy expression inhibitor of the GAL gene family, Mig1, to repress unwanted genes in the presence of elevated extracellular glucose concentrations...
  10. Schultz Larsen T, Lenk A, Kalinowska K, Vestergaard L, Pedersen C, Isono E, et al. The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Rep. 2018;25:2329-2338.e5 pubmed publisher
    ..Co-expressing the autoactive RPM1D505V and the catalytically inactive ESCRT-III protein SKD1E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-..
  11. O Donnell A, McCartney R, Chandrashekarappa D, Zhang B, Thorner J, Schmidt M. 2-Deoxyglucose impairs Saccharomyces cerevisiae growth by stimulating Snf1-regulated and α-arrestin-mediated trafficking of hexose transporters 1 and 3. Mol Cell Biol. 2015;35:939-55 pubmed publisher
    ..by 2DG phosphorylates Rod1 but fails to phosphorylate other known targets, such as the transcriptional repressor Mig1. We propose a novel mechanism for 2DG-induced toxicity whereby 2DG stimulates the modification of α-arrestins, ..
  12. Gao J, Yuan W, Li Y, Bai F, Jiang Y. Characterization of inulinase promoter from Kluyveromyces marxianus for intensive protein expression in industrial biotechnology. FEMS Yeast Res. 2017;17: pubmed publisher
    ..Responses of the KmINU1 promoter to carbon source were shown to be related to the Mig1 binding site extending from -496 to -485 bp...
  13. Hu A, Lu T, Chen D, Huang J, Feng W, Li Y, et al. Vps4b heterozygous mice do not develop tooth defects that replicate human dentin dysplasia I. BMC Genet. 2019;20:7 pubmed publisher
    Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the ATP enzyme AAA protein family, and is mainly involved in protein degradation and cell membrane fusion...
  14. Nurcholis M, Nitiyon S, Suprayogi -, Rodrussamee N, Lertwattanasakul N, Limtong S, et al. Functional analysis of Mig1 and Rag5 as expressional regulators in thermotolerant yeast Kluyveromyces marxianus. Appl Microbiol Biotechnol. 2019;103:395-410 pubmed publisher
    ..glucose repression mechanism in the thermotolerant yeast Kluyveromyces marxianus, disrupted mutants of genes for Mig1 and Rag5 as orthologs of Mig1 and Hxk2, respectively, in Saccharomyces cerevisiae were constructed, and their ..
  15. Cottier F, Tan A, Xu X, Wang Y, Pavelka N. MIG1 Regulates Resistance of Candida albicans against the Fungistatic Effect of Weak Organic Acids. Eukaryot Cell. 2015;14:1054-61 pubmed publisher
    ..albicans to one or more WOAs. A single gene, MIG1, previously known for its role in glucose repression, conferred resistance against all four acids tested...
  16. Kobbe R, Kolster M, Fuchs S, Schulze Sturm U, Jenderny J, Kochhan L, et al. Common variable immunodeficiency, impaired neurological development and reduced numbers of T regulatory cells in a 10-year-old boy with a STAT1 gain-of-function mutation. Gene. 2016;586:234-8 pubmed publisher
    ..IFN?) and/or IFN? resulted in a significantly increased expression of STAT1-regulated target genes such as MIG1, IRF1, MX1, MCP1/CCL2, IFI-56K, and CXCL10 as compared to IFN-treated cells from a healthy control, while no IFN?/?-..
  17. Jiang D, Hu B, Wei L, Xiong Y, Wang G, Ni T, et al. High expression of vacuolar protein sorting 4B (VPS4B) is associated with accelerated cell proliferation and poor prognosis in human hepatocellular carcinoma. Pathol Res Pract. 2015;211:240-7 pubmed publisher
    b>Vacuolar protein sorting 4B (VPS4B) is a member of ATPase family proteins that have been shown to play important roles in the formation of MVBs, virus budding and abscission of cytokinesis...
  18. Cupertino F, Virgilio S, Freitas F, Candido T, Bertolini M. Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator. Fungal Genet Biol. 2015;77:82-94 pubmed publisher
    The transcription factor CreA/Mig1/CRE-1 is a repressor protein that regulates the use of alternative carbon sources via a mechanism known as Carbon Catabolite Repression (CCR)...
  19. Cesaro L, Toffoletto M, Calistri A, Salvi M. Dissecting the Role of K61/K59 Residue in VPS4 Functions. Protein Pept Lett. 2016;23:518-24 pubmed
    ..events in which VPS4 is involved, here we provide evidence that the K61 residue, mapping within the MIT domain of VPS4B (K59 in VPS4A), is involved in VPS4 functioning...
  20. Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, et al. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000;11:747-63 pubmed
    The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole...
  21. McCartney R, Chandrashekarappa D, Zhang B, Schmidt M. Genetic analysis of resistance and sensitivity to 2-deoxyglucose in Saccharomyces cerevisiae. Genetics. 2014;198:635-46 pubmed publisher
    ..Snf1 kinase activated by 2-deoxyglucose does not phosphorylate the Mig1 protein, a known Snf1 substrate during glucose limitation...
  22. Jiang B, Himmelsbach K, Ren H, Boller K, Hildt E. Subviral Hepatitis B Virus Filaments, like Infectious Viral Particles, Are Released via Multivesicular Bodies. J Virol. 2015;90:3330-41 pubmed publisher
    ..inhibitor U18666A or inhibition of ESCRT functionality by coexpression of transdominant negative mutants (Vps4A, Vps4B, and CHMP3) abolishes the release of filaments while the secretion of spheres is not affected...
  23. Mi S, Qin X, Lin Y, He J, Chen N, Liu C, et al. Budding of Tiger Frog Virus (an Iridovirus) from HepG2 Cells via Three Ways Recruits the ESCRT Pathway. Sci Rep. 2016;6:26581 pubmed publisher
    ..Inhibition of class E proteins and auxiliary proteins (VPS4A, VPS4B, Tsg101, Alix, and Nedd4...
  24. Shashkova S, Welkenhuysen N, Hohmann S. Molecular communication: crosstalk between the Snf1 and other signaling pathways. FEMS Yeast Res. 2015;15:fov026 pubmed publisher
    ..The main glucose repression pathway, Snf1/Mig1, plays an essential role in adaptation to glucose limitation...
  25. Balderas Hernández V, Correia K, Mahadevan R. Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid. J Ind Microbiol Biotechnol. 2018;45:735-751 pubmed publisher
    ..75 g/L (initial pH not adjusted), the m9 strain (Δmig1::kan) produced 4.06 ± 0.14 and 3.87 ± 0.06 g/L of ethanol, respectively...
  26. Almquist J, Bendrioua L, Adiels C, Goksör M, Hohmann S, Jirstrand M. A Nonlinear Mixed Effects Approach for Modeling the Cell-To-Cell Variability of Mig1 Dynamics in Yeast. PLoS ONE. 2015;10:e0124050 pubmed publisher
    ..of NLME modeling to study cell-to-cell variability in the dynamic behavior of the yeast transcription repressor Mig1. In particular, we investigate a recently discovered phenomenon where Mig1 during a short and transient period ..
  27. Córdova P, Alcaíno J, Bravo N, Barahona S, Sepulveda D, Fernández Lobato M, et al. Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression. Microb Cell Fact. 2016;15:193 pubmed
    ..repression is mainly observed at the transcriptional level and depends on the DNA-binding regulator Mig1, which recruits the co-repressor complex Cyc8-Tup1, which then represses the expression of target genes...
  28. Baek S, Kwon E, Kim S, Hahn J. GSF2 deletion increases lactic acid production by alleviating glucose repression in Saccharomyces cerevisiae. Sci Rep. 2016;6:34812 pubmed publisher
    ..Accordingly, alleviation of glucose repression by deleting MIG1 or HXK2 in JHY5210 also improved D-LA production...
  29. Laera L, Guaragnella N, Zdralević M, Marzulli D, Liu Z, Giannattasio S. The transcription factors ADR1 or CAT8 are required for RTG pathway activation and evasion from yeast acetic acid-induced programmed cell death in raffinose. Microb Cell. 2016;3:621-631 pubmed publisher
    ..and double mutants lacking RTG2 and/or certain factors regulating carbon source utilization, including MIG1, HXK2, ADR1, CAT8, and HAP4, have been analyzed for their survival and CIT2 ..
  30. Shi L, Wang X, Wang J, Zhang P, Qi F, Cai M, et al. Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris. Genes Genomics. 2018;40:399-412 pubmed publisher
    Two catabolite repressor genes (MIG1 and MIG2) were previously identified in Pichia pastoris, and the derepression of alcohol oxidase (AOX) expression was realized in Δmig1 or Δmig1Δmig2 mutants grown in glycerol, but not in glucose...
  31. Granados A, Pietsch J, Cepeda Humerez S, Farquhar I, Tkacik G, Swain P. Distributed and dynamic intracellular organization of extracellular information. Proc Natl Acad Sci U S A. 2018;115:6088-6093 pubmed publisher
    ..and Sfp1) can encode the nature of multiple stresses, but only if stress is high; specialists (Hog1, Yap1, and Mig1/2) encode one particular stress, but do so more quickly and for a wider range of magnitudes...
  32. Vega M, Riera A, Fernández Cid A, Herrero P, Moreno F. Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex. J Biol Chem. 2016;291:7267-85 pubmed publisher
    ..theSUC2promoter context, Hxk2 functions through a variety of structurally unrelated factors, mainly the DNA-binding Mig1 and Mig2 repressors and the regulatory Snf1 and Reg1 factors...
  33. Friis R, Schultz M. Attenuation of transcriptional and signaling responses limits viability of ?(0)Saccharomyces cerevisiae during periods of glucose deprivation. Biochim Biophys Acta. 2016;1860:2563-2575 pubmed publisher
    ..Genes regulated by the transcription factors Mig1, Msn2, Gat1, and Ume6 were noticeably affected and phosphorylation of these factors in response to nutrient ..
  34. Schwenk B, Hartmann H, Serdaroglu A, Schludi M, Hornburg D, Meissner F, et al. TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons. EMBO J. 2016;35:2350-2370 pubmed
    ..levels in patient CSF Whole proteome quantification identified the upregulation of the ESCRT component VPS4B upon TDP-43 knockdown in neurons...
  35. Ren J, Yuan D, Xie L, Tao X, Duan C, Bao Y, et al. Up-regulation of Vps4A promotes neuronal apoptosis after intracerebral hemorrhage in adult rats. Metab Brain Dis. 2017;32:565-575 pubmed publisher
    ..belongs to ATPases Associated with diverse cellular Activities (AAA) protein family which is made up of Vps4A and Vps4B. Previous studies demonstrated that Vps4A plays vital roles in diverse aspects such as virus budding, the ..
  36. Ali A, Jadhav A, Jangid P, Patil R, Shelar A, Karuppayil S. The human muscarinic acetylcholine receptor antagonist, Dicyclomine targets signal transduction genes and inhibits the virulence factors in the human pathogen, Candida albicans. J Antibiot (Tokyo). 2018;71:456-466 pubmed publisher
    ..Eight genes were upregulated. Out of these, three major upregulated genes were Bcy1, Tup1, and Mig1. Dicyclomine downregulated Ume6, Ece1, and Pde2 genes which are involved in cAMP signaling pathway and also ..
  37. Amigoni L, Frigerio G, Martegani E, Colombo S. Involvement of Aif1 in apoptosis triggered by lack of Hxk2 in the yeast Saccharomyces cerevisiae. FEMS Yeast Res. 2016;16: pubmed publisher
    ..factor, besides its known role as a glycolytic enzyme and as a regulator of gene transcription of several Mig1-regulated genes. We also demonstrated that apoptosis induced by lack of Hxk2 may not require the activation of Yca1...
  38. Lambertini M, Ceppi M, Cognetti F, Cavazzini G, De Laurentiis M, De Placido S, et al. Dose-dense adjuvant chemotherapy in premenopausal breast cancer patients: A pooled analysis of the MIG1 and GIM2 phase III studies. Eur J Cancer. 2017;71:34-42 pubmed publisher
    ..In the MIG1 study, node-positive or high-risk node-negative patients were randomised to 6 cycles of fluorouracil/epirubicin/..
  39. Welkenhuysen N, Borgqvist J, Backman M, Bendrioua L, Goksör M, Adiels C, et al. Single-cell study links metabolism with nutrient signaling and reveals sources of variability. BMC Syst Biol. 2017;11:59 pubmed publisher
    ..When glucose becomes limited, the Snf1 kinase is activated and phosphorylates the transcriptional repressor Mig1, which is then exported from the nucleus...
  40. Buono R, Paez Valencia J, Miller N, Goodman K, Spitzer C, Spalding E, et al. Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development. Plant Physiol. 2016;171:251-64 pubmed publisher
    b>SKD1 is a core component of the mechanism that degrades plasma membrane proteins via the Endosomal Sorting Complex Required for Transport (ESCRT) pathway...
  41. Rippert D, Backhaus K, Rodicio R, Heinisch J. Cell wall synthesis and central carbohydrate metabolism are interconnected by the SNF1/Mig1 pathway in Kluyveromyces lactis. Eur J Cell Biol. 2017;96:70-81 pubmed publisher
    ..Application of cell wall stress shifted this equilibrium towards the non-phosphorylated fraction of KlMig1p. We conclude that KlMig1p may exert a negative regulatory function on cell wall biosynthesis. ..
  42. Lin Y, Sohn C, Dalal C, Cai L, Elowitz M. Combinatorial gene regulation by modulation of relative pulse timing. Nature. 2015;527:54-8 pubmed publisher
    ..time-lapse imaging of Saccharomyces cerevisiae, we show that the pulsatile transcription factors Msn2 and Mig1 combinatorially regulate their target genes through modulation of their relative pulse timing...
  43. Diezmann S, Leach M, Cowen L. Functional Divergence of Hsp90 Genetic Interactions in Biofilm and Planktonic Cellular States. PLoS ONE. 2015;10:e0137947 pubmed publisher
    ..Here, we explored the relationship between Hsp90 and five of these transcription factor genetic interactors: BCR1, MIG1, TEC1, TUP1, and UPC2...
  44. Alcaíno J, Bravo N, Córdova P, Marcoleta A, Contreras G, Barahona S, et al. The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production. PLoS ONE. 2016;11:e0162838 pubmed publisher
    ..dendrorhous MIG1 gene encoding the catabolic repressor Mig1, which mediates transcriptional glucose-dependent repression in other ..
  45. Wang F, Yang Y, Wang Z, Zhou J, Fan B, Chen Z. A Critical Role of Lyst-Interacting Protein5, a Positive Regulator of Multivesicular Body Biogenesis, in Plant Responses to Heat and Salt Stresses. Plant Physiol. 2015;169:497-511 pubmed publisher
    ..Lyst-Interacting Protein5 (LIP5), a positive regulator of the Suppressor of K(+) Transport Growth Defect1 (SKD1) AAA ATPase in MVB biogenesis, is a critical target of the mitogen-activated protein kinases MPK3 and MPK6 and ..
  46. Heck C, Kuhn H, Heidt S, Walter S, Rieger N, Requena N. Symbiotic Fungi Control Plant Root Cortex Development through the Novel GRAS Transcription Factor MIG1. Curr Biol. 2016;26:2770-2778 pubmed publisher
    ..to modulate root cortex development in Medicago truncatula by activating a novel GRAS-domain transcription factor, MIG1, that determines the size of cortical root cells...
  47. Watanabe R, Lamb R. Influenza virus budding does not require a functional AAA+ ATPase, VPS4. Virus Res. 2010;153:58-63 pubmed publisher
    ..It was found that neither siRNA knockdown of VPS4A and VPS4B expression nor the use of cell lines that inducibly express VPS4A or VPS4B dominant negative mutants, inhibited ..
  48. Shashkova S, Wollman A, Leake M, Hohmann S. The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms. FEMS Microbiol Lett. 2017;364: pubmed publisher
    ..derepression of genes required for utilization of alternative carbon sources through the transcriptional repressor Mig1. It has been suggested that the Glc7-Reg1 phosphatase dephosphorylates Mig1...
  49. Beyer A, Scheuring S, Müller S, Mincheva A, Lichter P, Köhrer K. Comparative sequence and expression analyses of four mammalian VPS4 genes. Gene. 2003;305:47-59 pubmed
    ..like yeast only one VPS4 representative is present, we found that mammals harbour two paralogues, VPS4a and VPS4b. Most interestingly, the Fugu fish contains a third VPS4 paralogue (VPS4c)...
  50. Takasu H, Jee J, Ohno A, Goda N, Fujiwara K, Tochio H, et al. Structural characterization of the MIT domain from human Vps4b. Biochem Biophys Res Commun. 2005;334:460-5 pubmed
    ..Here, we have determined the solution structure of the MIT domain isolated from the NH(2)-terminus of human Vps4b, an AAA-ATPase involved in multivesicular body formation...
  51. Welsch S, Habermann A, Jäger S, Muller B, Krijnse Locker J, Kräusslich H. Ultrastructural analysis of ESCRT proteins suggests a role for endosome-associated tubular-vesicular membranes in ESCRT function. Traffic. 2006;7:1551-66 pubmed
    ..These results represent the first systematic ultrastructural localization of ESCRT and provide insights into its role in uninfected and HIV-infected cells. ..
  52. Silvestri L, Ruthel G, Kallstrom G, Warfield K, Swenson D, Nelle T, et al. Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction. J Infect Dis. 2007;196 Suppl 2:S264-70 pubmed
    ..These data show that EBOV can use vps proteins independently of TSG101 for budding and reveal VPS4 as a potential target for filovirus therapeutics. ..
  53. Inoue M, Kamikubo H, Kataoka M, Kato R, Yoshimori T, Wakatsuki S, et al. Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B. Traffic. 2008;9:2180-9 pubmed publisher
    SKD1/VPS4B belongs to the adenosine triphosphatases associated with diverse cellular activities (AAA) family and regulates multivesicular body (MVB) biogenesis...
  54. Bruce E, Medcalf L, Crump C, Noton S, Stuart A, Wise H, et al. Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway. Virology. 2009;390:268-78 pubmed publisher
    ..Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. ..
  55. McDonough C, Bostrom M, Lu L, Hicks P, Langefeld C, Divers J, et al. Genetic analysis of diabetic nephropathy on chromosome 18 in African Americans: linkage analysis and dense SNP mapping. Hum Genet. 2009;126:805-17 pubmed publisher
    ..0017; OR (95% CI) = 0.65 (0.50-0.85)]. Collectively, these results suggest that multiple genes in this region may influence diabetic nephropathy susceptibility in AAs. ..
  56. Gan X, Gould S. Identification of an inhibitory budding signal that blocks the release of HIV particles and exosome/microvesicle proteins. Mol Biol Cell. 2011;22:817-30 pubmed publisher
    ..However, the IBS does inhibit an interaction between EMV cargo proteins and VPS4B, a component of the endosomal sorting complexes required for transport (ESCRT) machinery...
  57. Liu Y, Lv L, Xue Q, Wan C, Ni T, Chen B, et al. Vacuolar protein sorting 4B, an ATPase protein positively regulates the progression of NSCLC via promoting cell division. Mol Cell Biochem. 2013;381:163-71 pubmed publisher
    b>Vacuolar protein sorting 4B (VPS4B), a member of ATPase family proteins, plays a crucial role in lysosome-dependent degradation...
  58. Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, et al. Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun. 2014;5:5646 pubmed publisher
    ..Lack of ALG-2, ALIX or Vps4B each prevents shedding, and repair of the injured cell membrane...
  59. Yang Q, Chen D, Xiong F, Chen D, Liu C, Liu Y, et al. A splicing mutation in VPS4B causes dentin dysplasia I. J Med Genet. 2016;53:624-33 pubmed publisher
    ..33. We then identified a mutation IVS7+46C>G which resulted in a novel donor splice site in intron 7 of the VPS4B gene with co-segregation of all 10 affected individuals in this family...
  60. Xu L, Zhai L, Ge Q, Liu Z, Tao R. Vacuolar Protein Sorting 4B (VPS4B) Regulates Apoptosis of Chondrocytes via p38 Mitogen-Activated Protein Kinases (MAPK) in Osteoarthritis. Inflammation. 2017;40:1924-1932 pubmed publisher
    To aim of this study is to investigate the expression of VPS4B (vacuolar protein sorting 4B) in articular cartilage with osteoarthritis (OA) and to analyze the relationship between VPS4B and chondrocyte apoptosis...
  61. Duffy M, Alonso Padilla J, John L, Chandra N, Khan S, Ballmann M, et al. Generation and characterization of a novel candidate gene therapy and vaccination vector based on human species D adenovirus type 56. J Gen Virol. 2018;99:135-147 pubmed publisher
    ..inflammatory and cellular immune responses, with higher induction of IFNγ, TNFα, IL5, IL6, IP10, MCP1 and MIG1 compared to HAdV-5 following i.v. administration...
  62. Howard T, Stauffer D, Degnin C, Hollenberg S. CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci. 2001;114:2395-404 pubmed
    ..and biochemical fractionation localize CHMP1 to early endosomes and CHMP1 physically interacts with SKD1/VPS4, a highly conserved protein directly linked to multivesicular body sorting in yeast...
  63. Scheuring S, Röhricht R, Schöning Burkhardt B, Beyer A, Muller S, Abts H, et al. Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking. J Mol Biol. 2001;312:469-80 pubmed
    ..Based on these results, we conclude that both human VPS4 proteins are involved in intracellular protein trafficking, presumably at a late endosomal protein transport step, similar to the Vps4p in yeast. ..
  64. Ohsaki Y, Sugimoto Y, Suzuki M, Hosokawa H, Yoshimori T, Davies J, et al. Cholesterol depletion facilitates ubiquitylation of NPC1 and its association with SKD1/Vps4. J Cell Sci. 2006;119:2643-53 pubmed
    ..Another mutant, NPC1(deltaLLNF), which lacks the endosomal-targeting motif, also failed to respond. SKD1(E235Q), a dominant-negative mutant of SKD1/Vps4 that inhibits disassembly of the endosomal sorting complex required ..
  65. Pincetic A, Kuang Z, Seo E, Leis J. The interferon-induced gene ISG15 blocks retrovirus release from cells late in the budding process. J Virol. 2010;84:4725-36 pubmed publisher
    ..Thus, there appear to be multiple levels of ISG15-induced inhibition acting at different stages of the virus release process. ..
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    ..constructs revealed budding requirements for the following ESCRT proteins: ALIX, CHMP4B, CHMP2A and VPS4A or VPS4B. EIAV budding was inhibited by point mutations that abrogate the direct interactions between ALIX:CHMP4B, CHMP4B:..
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    ..The effect of low glucose and alkaline stresses was examined for two Snf1 phosphorylation substrates, the Mig1 and Mig2 proteins. Any of the three isoforms was capable of phosphorylating Mig1 in response to glucose stress...
  70. Wang Z, Bai X, Guo X, He X. Regulation of crucial enzymes and transcription factors on 2-phenylethanol biosynthesis via Ehrlich pathway in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2017;44:129-139 pubmed publisher
    ..Furthermore, influence of transcription factors Cat8 and Mig1 on 2-PE biosynthesis was explored...
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    ..in the presence of glucose as carbon source, the regulation of each PKA promoter subunits is different and only TPK1 is repressed by the complex Hxk2/Mig1 in the presence of active Snf1. Copyright © 2017 John Wiley & Sons, Ltd.
  72. Fujita H, Umezuki Y, Imamura K, Ishikawa D, Uchimura S, Nara A, et al. Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B. J Cell Sci. 2004;117:2997-3009 pubmed
    b>SKD1 belongs to the AAA-ATPase family and is one of the mammalian class E Vps (vacuolar protein sorting) proteins...
  73. Balasubramaniam M, Freed E. New insights into HIV assembly and trafficking. Physiology (Bethesda). 2011;26:236-51 pubmed publisher
    ..In this review, we highlight the current understanding of the cellular machinery involved in Gag trafficking and virus assembly. ..
  74. Xia J, Wang W, Li L, Liu Z, Liu M, Yang D. Inhibition of HBV replication by VPS4B and its dominant negative mutant VPS4B-K180Q in vivo. J Huazhong Univ Sci Technolog Med Sci. 2012;32:311-316 pubmed publisher
    This study examined the anti-hepatitis B virus (HBV) effect of wild-type (WT) vacuolar protein sorting 4B (VPS4B) and its dominant negative (DN) mutant VPS4B-K180Q in vivo in order to further explore the relationship between HBV and the ..
  75. Zhang D, Wang L, Yan L, Miao X, Gong C, Xiao M, et al. Vacuolar protein sorting 4B regulates apoptosis of intestinal epithelial cells via p38 MAPK in Crohn's disease. Exp Mol Pathol. 2015;98:55-64 pubmed publisher
    b>Vacuolar protein sorting 4B (VPS4B), a member of ATPase family proteins, reportedly possesses multiple biological functions, such as regulating the development of breast cancer and non-small-cell lung cancer, participating in Parkinson's ..
  76. Vild C, Li Y, Guo E, Liu Y, Xu Z. A novel mechanism of regulating the ATPase VPS4 by its cofactor LIP5 and the endosomal sorting complex required for transport (ESCRT)-III protein CHMP5. J Biol Chem. 2015;290:7291-303 pubmed publisher
    ..Together, these results suggest a novel mechanism of VPS4 regulation in metazoans, where CHMP5 functions as a negative allosteric switch to control LIP5-mediated stimulation of VPS4. ..
  77. Zhang Y, Sun J, Li F, Grogan T, Vergara J, Luan Q, et al. Salivary extracellular RNA biomarkers for insulin resistance detection in hispanics. Diabetes Res Clin Pract. 2017;132:85-94 pubmed publisher
    ..From the top ten candidates, six were individually validated (PRKCB, S100A12, IL1R2, CAMP, VPS4B, CAP1) (p<0.01) and yielded AUC values ranging from 0.66 to 0.76...
  78. Sirr A, Scott A, Cromie G, Ludlow C, Ahyong V, Morgan T, et al. Natural Variation in SER1 and ENA6 Underlie Condition-Specific Growth Defects in Saccharomyces cerevisiae. G3 (Bethesda). 2018;8:239-251 pubmed publisher
    ..driven by variation in the ENA6 promoter, which harbors a deletion that removes binding sites for the Mig1 and Nrg1 transcriptional repressors...
  79. Scheuring S, Bodor O, Röhricht R, Muller S, Beyer A, Köhrer K. Cloning, characterisation, and functional expression of the Mus musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and the yeast VPS4 genes are orthologues and involved in intracellular protein trafficking. Gene. 1999;234:149-59 pubmed
    The mouse SKD1 protein displays a high degree of sequence identity (62%) to the yeast Vps4 protein, which is involved in the transport of proteins out of a prevacuolar/endosomal compartment...
  80. Urata S, Yokosawa H, Yasuda J. Regulation of HTLV-1 Gag budding by Vps4A, Vps4B, and AIP1/Alix. Virol J. 2007;4:66 pubmed
    ..Here, we report that DN forms of Vps4A, Vps4B, and AIP1 inhibit HTLV-1 budding...
  81. Lin H, Li X, Chen J, Sun X, Cooper F, Chen Y, et al. Identification of an AAA ATPase VPS4B-dependent pathway that modulates epidermal growth factor receptor abundance and signaling during hypoxia. Mol Cell Biol. 2012;32:1124-38 pubmed publisher
    b>VPS4B, an AAA ATPase (ATPase associated with various cellular activities), participates in vesicular trafficking and autophagosome maturation in mammalian cells...
  82. Dores M, Chen B, Lin H, Soh U, Paing M, Montagne W, et al. ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting. J Cell Biol. 2012;197:407-19 pubmed publisher
    ..This study reveals a novel MVB/lysosomal sorting pathway for signaling receptors that bypasses the requirement for ubiquitination and ubiquitin-binding ESCRTs and may be applicable to a subset of GPCRs containing YPX(n)L motifs. ..
  83. Salvi M, Raiborg C, Hanson P, Campsteijn C, Stenmark H, Pinna L. CK2 involvement in ESCRT-III complex phosphorylation. Arch Biochem Biophys. 2014;545:83-91 pubmed publisher
    ..protein kinase CK2? is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly. This phosphorylation is observed both in vitro and in cells...
  84. Adamczyk M, Szatkowska R. Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS ONE. 2017;12:e0185516 pubmed publisher
    ..is controlled through finely tuned communication between multiple glucose sensing pathways including the Snf1-Mig1, Snf3/Rgt2-Rgt1 (SRR) and cAMP-PKA pathways...
  85. Katoh K, Suzuki H, Terasawa Y, Mizuno T, Yasuda J, Shibata H, et al. The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B. Biochem J. 2005;391:677-85 pubmed
    ..cells that overexpressed a GFP (green fluorescent protein)-fused ATPase-defective dominant-negative form of SKD1/Vps4B (GFP-SKD1(E235Q)), ALG-2 exhibited a punctate distribution at the perinuclear area and co-localized with GFP-SKD1(..
  86. Vande Walle L, Van Damme P, Lamkanfi M, Saelens X, Vandekerckhove J, Gevaert K, et al. Proteome-wide Identification of HtrA2/Omi Substrates. J Proteome Res. 2007;6:1006-15 pubmed
    ..Our results suggest that HtrA2/Omi has a rather narrow cleavage site preference and that cytoskeletal proteins are prime targets of this protease. ..
  87. Lambert C, Döring T, Prange R. Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin. J Virol. 2007;81:9050-60 pubmed
    ..Upon coexpression of mutated CHMP3, CHMP4B, or CHMP4C forms, as well as of ATPase-defective Vps4A or Vps4B mutants, HBV assembly and egress were potently blocked...
  88. daSilva L, Sougrat R, Burgos P, Janvier K, Mattera R, Bonifacino J. Human immunodeficiency virus type 1 Nef protein targets CD4 to the multivesicular body pathway. J Virol. 2009;83:6578-90 pubmed publisher
    ..We also show that both CD4 and Nef are ubiquitinated on lysine residues, but this modification is dispensable for Nef-induced targeting of CD4 to the MVB pathway. ..
  89. Colombo M, Moita C, van Niel G, Kowal J, Vigneron J, Benaroch P, et al. Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles. J Cell Sci. 2013;126:5553-65 pubmed publisher
    ..By contrast, depletion of VPS4B augmented this secretion while not altering the features of EVs...
  90. Tang J, Ji L, Wang Y, Huang Y, Yin H, He Y, et al. Cell adhesion down-regulates the expression of vacuolar protein sorting 4B (VPS4B) and contributes to drug resistance in multiple myeloma cells. Int J Hematol. 2015;102:25-34 pubmed publisher
    The expression and biologic function of the gene encoding vacuolar protein sorting 4B (VPS4B) in human multiple myeloma (MM) were investigated in this study...
  91. Cottier F, Tan A, Yurieva M, Liao W, Lum J, Poidinger M, et al. The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid. G3 (Bethesda). 2017;7:3597-3604 pubmed publisher
    ..albicans faces in its natural environment. Inactivation of the transcriptional regulator MIG1 in C. albicans results in a lack of sensitivity to this synergistic outcome. To decipher C...
  92. Broxton C, He B, Bruno V, Culotta V. A role for Candida albicans superoxide dismutase enzymes in glucose signaling. Biochem Biophys Res Commun. 2018;495:814-820 pubmed publisher
    ..Instead C. albicans SODs work in the glucose repression pathway involving the MIG1 transcriptional repressor. In C. albicans, the SODs repress glucose uptake, while in S...