Gene Symbol: VPS4A
Description: vacuolar protein sorting 4 homolog A
Alias: SKD1, SKD1A, SKD2, VPS4, VPS4-1, vacuolar protein sorting-associated protein 4A, SKD1-homolog, hVPS4, vacuolar protein sorting factor 4A, vacuolar sorting protein 4
Species: human
Products:     VPS4A

Top Publications

  1. Garrus J, von Schwedler U, Pornillos O, Morham S, Zavitz K, Wang H, et al. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001;107:55-65 pubmed
    ..Dominant negative mutant Vps4 proteins that inhibit vacuolar protein sorting also arrest HIV-1 and MLV budding...
  2. Shim S, Merrill S, Hanson P. Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly. Mol Biol Cell. 2008;19:2661-72 pubmed publisher
    The AAA+ ATPase VPS4 plays an essential role in multivesicular body biogenesis and is thought to act by disassembling ESCRT-III complexes. VPS4 oligomerization and ATPase activity are promoted by binding to LIP5...
  3. Baietti M, Zhang Z, Mortier E, Melchior A, Degeest G, Geeraerts A, et al. Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14:677-85 pubmed publisher
    ..This study identifies a key role for syndecan-syntenin-ALIX in membrane transport and signalling processes. ..
  4. Howard T, Stauffer D, Degnin C, Hollenberg S. CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci. 2001;114:2395-404 pubmed
    ..and biochemical fractionation localize CHMP1 to early endosomes and CHMP1 physically interacts with SKD1/VPS4, a highly conserved protein directly linked to multivesicular body sorting in yeast...
  5. Martin Serrano J, Yarovoy A, Perez Caballero D, Bieniasz P, Yaravoy A. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003;100:12414-9 pubmed
  6. Stuchell Brereton M, Skalicky J, Kieffer C, Karren M, Ghaffarian S, Sundquist W. ESCRT-III recognition by VPS4 ATPases. Nature. 2007;449:740-4 pubmed
    ..Here we show that the microtubule interacting and transport (MIT) domains of human VPS4A and VPS4B bind conserved sequence motifs located at the carboxy termini of the CHMP1-3 class of ESCRT-III proteins...
  7. Baumgärtel V, Ivanchenko S, Dupont A, Sergeev M, Wiseman P, Kräusslich H, et al. Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component. Nat Cell Biol. 2011;13:469-74 pubmed publisher
    ..Using live-cell imaging, we show late-domain-dependent recruitment of VPS4A to nascent HIV particles at the host cell plasma membrane...
  8. Morita E, Sandrin V, Chung H, Morham S, Gygi S, Rodesch C, et al. Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 2007;26:4215-27 pubmed
    ..ESCRT-III and VPS4 proteins are also recruited, indicating that much of the ESCRT pathway localizes to the midbody...
  9. Jouvenet N, Zhadina M, Bieniasz P, Simon S. Dynamics of ESCRT protein recruitment during retroviral assembly. Nat Cell Biol. 2011;13:394-401 pubmed publisher
    ..to be sequentially recruited to the site of membrane scission, and then complexes are disassembled by the ATPase Vps4A. However, these events have never been observed in living cells, and their dynamics are unknown...

More Information

Publications136 found, 100 shown here

  1. Bishop N, Woodman P. ATPase-defective mammalian VPS4 localizes to aberrant endosomes and impairs cholesterol trafficking. Mol Biol Cell. 2000;11:227-39 pubmed
    ..mammalian VPS4 in endosomal trafficking, we have transiently expressed wild-type or ATPase-defective human VPS4 (hVPS4) in cultured cells...
  2. Scheuring S, Röhricht R, Schöning Burkhardt B, Beyer A, Muller S, Abts H, et al. Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking. J Mol Biol. 2001;312:469-80 pubmed
    The yeast Vps4 protein (Vps4p) is a member of the AAA protein family (ATPases associated with diverse cellular activities) and a key player in the transport of proteins out of a prevacuolar endosomal compartment...
  3. Strack B, Calistri A, Craig S, Popova E, Gottlinger H. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689-99 pubmed
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  4. von Schwedler U, Stuchell M, Muller B, Ward D, Chung H, Morita E, et al. The protein network of HIV budding. Cell. 2003;114:701-13 pubmed
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses. ..
  5. Marchese A, Raiborg C, Santini F, Keen J, Stenmark H, Benovic J. The E3 ubiquitin ligase AIP4 mediates ubiquitination and sorting of the G protein-coupled receptor CXCR4. Dev Cell. 2003;5:709-22 pubmed
    ..Endosomal sorting of CXCR4 is dependent on Hrs as well as the AAA ATPase Vps4, the latter involved in regulating the ubiquitination status of both CXCR4 and Hrs...
  6. Scott A, Gaspar J, Stuchell Brereton M, Alam S, Skalicky J, Sundquist W. Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A. Proc Natl Acad Sci U S A. 2005;102:13813-8 pubmed
    ..Here, we report the solution structure of the N-terminal VPS4A microtubule interacting and transport (MIT) domain and demonstrate that the VPS4A MIT domain binds the C-terminal ..
  7. Scott A, Chung H, Gonciarz Swiatek M, Hill G, Whitby F, Gaspar J, et al. Structural and mechanistic studies of VPS4 proteins. EMBO J. 2005;24:3658-69 pubmed
    ..Here, we report the crystal structure of monomeric apo human VPS4B/SKD1 (hVPS4B), which is composed of five distinct elements: a poorly ordered N-terminal MIT domain that binds ESCRT-III ..
  8. Langelier C, von Schwedler U, Fisher R, De Domenico I, White P, Hill C, et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006;80:9465-80 pubmed
    ..with its expected role in MVB vesicle formation, (i) human ESCRT-II localized to endosomal membranes in a VPS4-dependent fashion and (ii) depletion of EAP20/ESCRT-II and CHMP6/ESCRT-III inhibited lysosomal targeting and ..
  9. Bajorek M, Morita E, Skalicky J, Morham S, Babst M, Sundquist W. Biochemical analyses of human IST1 and its function in cytokinesis. Mol Biol Cell. 2009;20:1360-73 pubmed publisher
    ..IST1 binding interactions with VPS4, CHMP1, LIP5, and ESCRT-I were characterized, and the IST1-VPS4 interaction was investigated in detail...
  10. Morita E, Colf L, Karren M, Sandrin V, Rodesch C, Sundquist W. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010;107:12889-94 pubmed publisher
    ..We found that depletion of VPS4A, VPS4B, or any of the 11 different human ESCRT-III (CHMP) proteins inhibited abscission...
  11. Lin Y, Kimpler L, Naismith T, Lauer J, Hanson P. Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1. J Biol Chem. 2005;280:12799-809 pubmed
    b>SKD1/VPS4B is an AAA+ (ATPase associated with a variety of cellular activities) protein involved in multivesicular body (MVB) biogenesis...
  12. Skalicky J, Arii J, Wenzel D, Stubblefield W, Katsuyama A, Uter N, et al. Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport. J Biol Chem. 2012;287:43910-26 pubmed publisher
    ..The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes, and the LIP5 protein helps ..
  13. Beyer A, Scheuring S, Müller S, Mincheva A, Lichter P, Köhrer K. Comparative sequence and expression analyses of four mammalian VPS4 genes. Gene. 2003;305:47-59 pubmed
    ..databases from completely or partially sequenced organisms revealed the existence of up to three paralogues, VPS4a, -b, and -c...
  14. Kieffer C, Skalicky J, Morita E, De Domenico I, Ward D, Kaplan J, et al. Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding. Dev Cell. 2008;15:62-73 pubmed publisher
    ..membrane-associated ESCRT-III proteins display terminal amphipathic "MIM1" helices that bind and recruit VPS4 ATPases via their MIT domains...
  15. Sundquist W, Kräusslich H. HIV-1 assembly, budding, and maturation. Cold Spring Harb Perspect Med. 2012;2:a006924 pubmed publisher
    ..Here, we review our current understanding of the mechanisms of HIV-1 assembly, budding, and maturation, starting with a general overview and then providing detailed descriptions of each of the different stages of virion morphogenesis. ..
  16. Vromman F, Perrinet S, Gehre L, Subtil A. The DUF582 Proteins of Chlamydia trachomatis Bind to Components of the ESCRT Machinery, Which Is Dispensable for Bacterial Growth In vitro. Front Cell Infect Microbiol. 2016;6:123 pubmed
    ..at different stages of ESCRT-driven processes, or inhibition by expression of a dominant negative mutant of VPS4, we demonstrated that this machinery was dispensable for bacterial entry, multiplication and differentiation into ..
  17. Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
    ..5- to 1.0-micrometer microvesicles (MVs) generated by vacuolar protein sorting 4 (VPS4). Antigen sharing by DCs is not limited to MCs, as neighboring DCs also acquire antigen-bearing MVs...
  18. Zhang C, Lai M, Khandan L, Lee L, Chen Z, Junge H. Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Nat Commun. 2017;8:16050 pubmed publisher
    ..body (MVB) pathway using a dominant negative ESCRT (endosomal sorting complexes required for transport) component VPS4 EQ strongly impairs NDP/FZD4 signalling in vitro and recapitulates CNS angiogenesis and blood-CNS-barrier defects ..
  19. DeMarino C, Pleet M, Cowen M, Barclay R, Akpamagbo Y, Erickson J, et al. Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1-Infected Cells. Sci Rep. 2018;8:7653 pubmed publisher
    ..we describe unique mechanisms of ESCRT pathway manipulation by antivirals, specifically the targeting of VPS4. Collectively, these data imply that, despite antiretroviral therapy, EVs containing viral products are continually ..
  20. Agromayor M, Martin Serrano J. Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo. J Biol Chem. 2006;281:23083-91 pubmed
    ..Finally, VPS4 and AMSH compete for binding to the C-terminal regions of CHMP1A and CHMP1B, revealing a coordinated interaction ..
  21. McCullough J, Fisher R, Whitby F, Sundquist W, Hill C. ALIX-CHMP4 interactions in the human ESCRT pathway. Proc Natl Acad Sci U S A. 2008;105:7687-91 pubmed publisher
    ..different ESCRT-III subunits to bind different ESCRT pathway partners, with CHMP1-3 proteins binding MIT domain-containing proteins, such as VPS4 and Vta1/LIP5, and CHMP4 proteins binding Bro1 domain-containing proteins, such as ALIX.
  22. Urata S, Weyer J, Storm N, Miyazaki Y, van Vuren P, Paweska J, et al. Analysis of Assembly and Budding of Lujo Virus. J Virol. 2015;90:3257-61 pubmed publisher
    ..The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding...
  23. Goliand I, Adar Levor S, Segal I, Nachmias D, Dadosh T, Kozlov M, et al. Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM. Cell Rep. 2018;24:1756-1764 pubmed publisher
    ..the microtubule-severing enzyme spastin and in cells depleted of specific ESCRT-III components or the ATPase VPS4 demonstrated the contribution of these components to the organization and function of ESCRTs in cells...
  24. Schöneberg J, Pavlin M, Yan S, Righini M, Lee I, Carlson L, et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science. 2018;362:1423-1428 pubmed publisher
    ..We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled...
  25. Yang D, Hurley J. Structural role of the Vps4-Vta1 interface in ESCRT-III recycling. Structure. 2010;18:976-84 pubmed publisher
    ..The final step in the ESCRT cycle is the disassembly of the ESCRT-III lattice by the AAA+ ATPase Vps4. Vps4 assembles on its membrane-bound ESCRT-III substrate with its cofactor, Vta1...
  26. Thoresen S, Campsteijn C, Vietri M, Schink K, Liestøl K, Andersen J, et al. ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4. Nat Cell Biol. 2014;16:550-60 pubmed publisher
    ..of the abscission checkpoint, functioning through the most downstream component of the ESCRT machinery, the ATPase VPS4. In concert with CHMP4C, ANCHR associates with VPS4 at the midbody ring following DNA segregation defects to ..
  27. Legent K, Liu H, Treisman J. Drosophila Vps4 promotes Epidermal growth factor receptor signaling independently of its role in receptor degradation. Development. 2015;142:1480-91 pubmed publisher
    ..screen for mutations that affect Drosophila eye development, we identified an allele of Vacuolar protein sorting 4 (Vps4), which encodes an AAA ATPase that interacts with the ESCRT-III complex to drive the final step of ILV formation...
  28. Hurley J. The ESCRT complexes. Crit Rev Biochem Mol Biol. 2010;45:463-87 pubmed publisher
    The ESCRT machinery consists of the peripheral membrane protein complexes ESCRT-0, -I, -II, -III, and Vps4-Vta1, and the ALIX homodimer...
  29. Hamdan F, Srour M, Capo Chichi J, Daoud H, Nassif C, Patry L, et al. De novo mutations in moderate or severe intellectual disability. PLoS Genet. 2014;10:e1004772 pubmed publisher
    ..12 possibly pathogenic DNMs in genes (HNRNPU, WAC, RYR2, SET, EGR1, MYH10, EIF2C1, COL4A3BP, CHMP2A, PPP1CB, VPS4A, PPP2R2B) that have not previously been causally linked to ID...
  30. Yang B, Stjepanovic G, Shen Q, Martin A, Hurley J. Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation. Nat Struct Mol Biol. 2015;22:492-8 pubmed publisher
    The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation...
  31. Yoshikawa R, Miyaho R, Hashimoto A, Abe M, Yasuda J, Miyazawa T. Suppression of production of baboon endogenous virus by dominant negative mutants of cellular factors involved in multivesicular body sorting pathway. Virus Res. 2015;196:128-34 pubmed publisher
    ..We also demonstrated that Vps4A/B are involved in BaEV budding...
  32. McNally F, Roll Mecak A. Microtubule-severing enzymes: From cellular functions to molecular mechanism. J Cell Biol. 2018;217:4057-4069 pubmed publisher
    ..enzymes, katanin, spastin, and fidgetin, are members of the meiotic subfamily of AAA ATPases that also includes VPS4, which disassembles ESCRTIII polymers...
  33. Schultz Larsen T, Lenk A, Kalinowska K, Vestergaard L, Pedersen C, Isono E, et al. The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Rep. 2018;25:2329-2338.e5 pubmed publisher
    ..Co-expressing the autoactive RPM1D505V and the catalytically inactive ESCRT-III protein SKD1E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-..
  34. Monroe N, Hill C. Meiotic Clade AAA ATPases: Protein Polymer Disassembly Machines. J Mol Biol. 2016;428:1897-911 pubmed publisher
    ..classification of their AAA ATPase cassette, include four relatively well characterized family members, Vps4, spastin, katanin and fidgetin...
  35. Jermy A. Virology: ESCRTing retroviruses to the exit. Nat Rev Microbiol. 2011;9:314 pubmed publisher
  36. Monroe N, Han H, Gonciarz M, Eckert D, Karren M, Whitby F, et al. The oligomeric state of the active Vps4 AAA ATPase. J Mol Biol. 2014;426:510-25 pubmed publisher
    ..A component of this pathway, the AAA ATPase Vps4, provides energy for pathway progression...
  37. Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
    ..its interaction partner CHMP4B are localised on endosomes in MEF cells, when the activity of the endosomal protein VPS4 is reduced. This indicates that CC2D1A cycles between the cytosol and the endosomal membrane...
  38. Amerik A, Nowak J, Swaminathan S, Hochstrasser M. The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell. 2000;11:3365-80 pubmed
    ..However, in cells lacking the Vps4/Did6 ATPase, a large fraction of Doa4-green fluorescent protein, like several other Vps factors, concentrates at ..
  39. Lambert C, Döring T, Prange R. Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin. J Virol. 2007;81:9050-60 pubmed
    ..Upon coexpression of mutated CHMP3, CHMP4B, or CHMP4C forms, as well as of ATPase-defective Vps4A or Vps4B mutants, HBV assembly and egress were potently blocked...
  40. Nara A, Mizushima N, Yamamoto A, Kabeya Y, Ohsumi Y, Yoshimori T. SKD1 AAA ATPase-dependent endosomal transport is involved in autolysosome formation. Cell Struct Funct. 2002;27:29-37 pubmed
    Mouse SKD1 AAA ATPase is involved in the sorting and transport from endosomes; cells overexpressing a dominant-negative mutant, SKD1(E235Q) were defective in endosomal transport to both the plasma membranes and lysosomes (Yoshimori et al...
  41. Kharkwal H, Smith C, Wilson D. Blocking ESCRT-mediated envelopment inhibits microtubule-dependent trafficking of alphaherpesviruses in vitro. J Virol. 2014;88:14467-78 pubmed publisher
    ..Here, we demonstrate that blocking ESCRT-mediated envelopment using the dominant-negative inhibitor Vps4A-EQ (Vps4A in which glutamate [E] at position 228 in the ATPase active site is replaced by a glutamine [Q]) reduced ..
  42. Yue Q, Yu Q, Yang Q, Xu Y, Guo Y, Blissard G, et al. Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus. J Virol. 2018;92: pubmed publisher
    ..Recently, it was demonstrated that Vps4, the key regulator for recycling of the ESCRT-III complex, is required for efficient infection by the baculovirus ..
  43. Webster B, Colombi P, Jäger J, Lusk C. Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4. Cell. 2014;159:388-401 pubmed publisher
    ..Heh2 recruits the endosomal sorting complex required for transport (ESCRT)-III subunit Snf7 and the AAA-ATPase Vps4 to destabilize and clear defective NPC assembly intermediates...
  44. Kubala M, Norwood S, Gomez G, Jones A, Johnston W, Yap A, et al. Mammalian farnesyltransferase α subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A)--dependent intracellular trafficking through recycling endosomes. Biochem Biophys Res Commun. 2015;468:580-6 pubmed publisher
    ..b>Vps4A was validated for direct binding to the FTase α subunit both in vitro and in vivo...
  45. Krasniak C, Ahmad S. The role of CHMP2BIntron5 in autophagy and frontotemporal dementia. Brain Res. 2016;1649:151-157 pubmed publisher
    ..ESCRT-III recruits Vps4, an AAA-ATPase that abscises the membrane during various cellular processes including autophagy and intraluminal ..
  46. Utley T, Ducharme N, Varthakavi V, Shepherd B, Santangelo P, Lindquist M, et al. Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. Proc Natl Acad Sci U S A. 2008;105:10209-14 pubmed publisher
    ..RSV budding was independent of the ESCRT machinery, the only well-defined budding mechanism for enveloped RNA viruses. Therefore, RSV uses a virus budding mechanism that is controlled by FIP2. ..
  47. Xia J, Wang W, Li L, Liu Z, Liu M, Yang D. Inhibition of HBV replication by VPS4B and its dominant negative mutant VPS4B-K180Q in vivo. J Huazhong Univ Sci Technolog Med Sci. 2012;32:311-316 pubmed publisher
    ..mutant VPS4B-K180Q in vivo in order to further explore the relationship between HBV and the host cellular factor VPS4. VPS4B gene was amplified from Huh7 cells by RT-PCR and cloned into the eukaryotic expression vector pXF3H...
  48. Sandrin V, Sundquist W. ESCRT requirements for EIAV budding. Retrovirology. 2013;10:104 pubmed publisher
    ..and mutant constructs revealed budding requirements for the following ESCRT proteins: ALIX, CHMP4B, CHMP2A and VPS4A or VPS4B...
  49. Steffens A, Jakoby M, Hulskamp M. Physical, Functional and Genetic Interactions between the BEACH Domain Protein SPIRRIG and LIP5 and SKD1 and Its Role in Endosomal Trafficking to the Vacuole in Arabidopsis. Front Plant Sci. 2017;8:1969 pubmed publisher
    ..sorting complex required for transport associated ATPase Suppressor of K+-transport growth defect1 (SKD1) and its positive regulator, LYST Interacting Protein 5 (LIP5) and report genetic interactions between SPI ..
  50. Liu J, Gao R, Li C, Ni J, Yang Z, Zhang Q, et al. Functional assignment of multiple ESCRT-III homologs in cell division and budding in Sulfolobus islandicus. Mol Microbiol. 2017;105:540-553 pubmed publisher
    ..This machinery comprises three proteins: CdvA, Eukaryotic-like ESCRT-III and Vps4. In addition to ESCRT-III, Sulfolobus cells also encode three other ESCRT-III homologs termed ESCRT-III-1, -2 and -..
  51. Liu Z, Cauvi D, Bernardino E, Lara B, Lizardo R, Hawisher D, et al. Isolation and characterization of human urine extracellular vesicles. Cell Stress Chaperones. 2018;23:943-953 pubmed publisher
    ..Several markers were found, including dipeptidyl peptidase IV (CD26), vacuolar protein sorting factor 4A (VPS4A) and dipeptidase 1 (DPEP1), and galectin 3 binding protein (G3-BP)...
  52. Takahashi Y, He H, Tang Z, Hattori T, Liu Y, Young M, et al. An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure. Nat Commun. 2018;9:2855 pubmed publisher
    ..Consistently, inhibition of the AAA-ATPase VPS4 activity impairs autophagosome completion...
  53. Koumanov F, Pereira V, Whitley P, Holman G. GLUT4 traffic through an ESCRT-III-dependent sorting compartment in adipocytes. PLoS ONE. 2012;7:e44141 pubmed publisher
    ..Introduction of the dominant negative inhibitory constructs of the ESCRT-III components CHMP3 (CHMP3(1-179)) and Vps4 (GFP-Vps4(E235Q)) into rat adipocytes leads to the accumulation of GLUT4 in large, coalesced and extended vesicles ..
  54. Popova E, Popov S, Gottlinger H. Human immunodeficiency virus type 1 nucleocapsid p1 confers ESCRT pathway dependence. J Virol. 2010;84:6590-7 pubmed publisher
    ..Together, these results indicate that the ESCRT pathway dependence of HIV-1 budding is determined, at least in part, by the NC-p1 region of Gag...
  55. Lee S, Jones J, Hardison S, Kot J, Khalique Z, Bernardo S, et al. Candida albicans VPS4 is required for secretion of aspartyl proteases and in vivo virulence. Mycopathologia. 2009;167:55-63 pubmed publisher
    ..We previously cloned and analyzed the C. albicans pre-vacuolar protein sorting gene VPS4, and demonstrated that extracellular Sap2p is absent in the culture supernatants of the vps4delta null mutant...
  56. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly...
  57. Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher
    b>Vps4 is a key enzyme that functions in endosomal protein trafficking, cytokinesis, and retroviral budding. Vps4 activity is regulated by its recruitment from the cytoplasm to ESCRT-III, where the protein oligomerizes into an active ATPase...
  58. Guo E, Xu Z. Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains. J Biol Chem. 2015;290:8396-408 pubmed publisher
    ..function of the ESCRT-III protein IST1, which binds to the microtubule interacting and trafficking (MIT) domains of VPS4, LIP5, and Spartin via its C-terminal MIT-interacting motif (MIM)...
  59. Tan J, Davies B, Payne J, Benson L, Katzmann D. Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation. J Biol Chem. 2015;290:30053-65 pubmed publisher
    ..The ATPase Vps4 remodels and disassembles ESCRT-III, but the manner in which Vps4 activity is coordinated with ESCRT-III function ..
  60. Mi S, Qin X, Lin Y, He J, Chen N, Liu C, et al. Budding of Tiger Frog Virus (an Iridovirus) from HepG2 Cells via Three Ways Recruits the ESCRT Pathway. Sci Rep. 2016;6:26581 pubmed publisher
    ..Inhibition of class E proteins and auxiliary proteins (VPS4A, VPS4B, Tsg101, Alix, and Nedd4...
  61. Cesaro L, Toffoletto M, Calistri A, Salvi M. Dissecting the Role of K61/K59 Residue in VPS4 Functions. Protein Pept Lett. 2016;23:518-24 pubmed
    ..VPS4 is involved, here we provide evidence that the K61 residue, mapping within the MIT domain of VPS4B (K59 in VPS4A), is involved in VPS4 functioning...
  62. Janvier K, Pelchen Matthews A, Renaud J, Caillet M, Marsh M, Berlioz Torrent C. The ESCRT-0 component HRS is required for HIV-1 Vpu-mediated BST-2/tetherin down-regulation. PLoS Pathog. 2011;7:e1001265 pubmed publisher
    ..Our results provide further insight into the mechanism by which Vpu counteracts BST-2/tetherin and promotes HIV-1 dissemination, and they highlight an additional role for the ESCRT machinery in virus release. ..
  63. Bänfer S, Schneider D, Dewes J, Strauss M, Freibert S, Heimerl T, et al. Molecular mechanism to recruit galectin-3 into multivesicular bodies for polarized exosomal secretion. Proc Natl Acad Sci U S A. 2018;115:E4396-E4405 pubmed publisher
    ..depends on the endosomal sorting complex required for transport I (ESCRT-I) component Tsg101 and functional Vps4a. Either Tsg101 knockdown or expression of dominant-negative Vps4aE228Q causes an intracellular Gal3 ..
  64. Scheuring S, Bodor O, Röhricht R, Muller S, Beyer A, Köhrer K. Cloning, characterisation, and functional expression of the Mus musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and the yeast VPS4 genes are orthologues and involved in intracellular protein trafficking. Gene. 1999;234:149-59 pubmed
    The mouse SKD1 protein displays a high degree of sequence identity (62%) to the yeast Vps4 protein, which is involved in the transport of proteins out of a prevacuolar/endosomal compartment...
  65. Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, et al. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000;11:747-63 pubmed
    The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole...
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    ..Taken together, these studies define the composition of human ESCRT-I complexes and indicate that the MVB12 subunits play a unique role in regulating ESCRT-mediated virus budding. ..
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    ..shown that ESCRT-III subunits polymerize rapidly on yeast endosomes, together with the recruitment of at least two Vps4 hexamers. During their 3-45 s lifetimes, the ESCRT-III assemblies accumulated 75-200 Snf7 and 15-50 Vps24 molecules...
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    ..inhibitor U18666A or inhibition of ESCRT functionality by coexpression of transdominant negative mutants (Vps4A, Vps4B, and CHMP3) abolishes the release of filaments while the secretion of spheres is not affected...
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    ..Further, our data showed that Vps4A, a key regulator of exosome biogenesis, was frequently down-regulated in HCC tissues...
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    ..We detected additional proteins (Alix, Nedd4 and the AAA-type ATPase VPS4) of the endosomal sorting complex required for transport (ESCRT) that are recruited into inclusions...
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    ..A similar effect was observed with a dominant-negative mutant of VPS4A cross-linked to a cell-penetrating peptide in nonpermeabilized sperm stimulated with a calcium ionophore...
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    ..for transport (ESCRT) machinery is an assembly of protein subcomplexes (ESCRT I-III) that cooperate with the ATPase VPS4 to mediate scission of membrane necks from the inside...
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    ..sorting 4, belongs to ATPases Associated with diverse cellular Activities (AAA) protein family which is made up of Vps4A and Vps4B...
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    ..The structural interface is twice as large as that of the MIT domain of the VPS4-CHMP complex, consistent with the high affinity of the interaction...
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    ..Lyst-Interacting Protein5 (LIP5), a positive regulator of the Suppressor of K(+) Transport Growth Defect1 (SKD1) AAA ATPase in MVB biogenesis, is a critical target of the mitogen-activated protein kinases MPK3 and MPK6 and ..
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    ..ESCRT-III turnover depended on the ATPase VPS4, which accumulated at the midbody simultaneously with ESCRT-III subunits, and was required for assembly of ..
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    ..By contrast, release was unaffected by depletion of some core ESCRTs, including VPS4. Reintroduction of ESCRT proteins to siRNA-depleted cells revealed interactions among ESCRT proteins that are ..
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