vitronectin

Summary

Gene Symbol: vitronectin
Description: vitronectin
Alias: V75, VNT, vitronectin, S-protein, complement S-protein, epibolin, serum spreading factor, somatomedin B
Species: human
Products:     vitronectin

Top Publications

  1. Fink T, Jenne D, Lichter P. The human vitronectin (complement S-protein) gene maps to the centromeric region of 17q. Hum Genet. 1992;88:569-72 pubmed
    b>Vitronectin (complement S-protein, serum-spreading factor, epibolin) is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to ..
  2. Kjaergaard M, Gardsvoll H, Hirschberg D, Nielbo S, Mayasundari A, Peterson C, et al. Solution structure of recombinant somatomedin B domain from vitronectin produced in Pichia pastoris. Protein Sci. 2007;16:1934-45 pubmed
    The cysteine-rich somatomedin B domain (SMB) of the matrix protein vitronectin is involved in several important biological processes...
  3. Schar C, Blouse G, Minor K, Peterson C. A deletion mutant of vitronectin lacking the somatomedin B domain exhibits residual plasminogen activator inhibitor-1-binding activity. J Biol Chem. 2008;283:10297-309 pubmed publisher
    ..The high affinity binding site for PAI-1 is located within the N-terminal somatomedin B domain of vitronectin; however, several studies have suggested a second PAI-1-binding site within vitronectin...
  4. Voss S, Hallström T, Saleh M, Burchhardt G, Pribyl T, Singh B, et al. The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin. J Biol Chem. 2013;288:15614-27 pubmed publisher
    ..Pneumococci engage vitronectin, the human adhesive glycoprotein and complement inhibitor, to facilitate attachment to epithelial cells of the ..
  5. Suzuki S, Oldberg A, Hayman E, Pierschbacher M, Ruoslahti E. Complete amino acid sequence of human vitronectin deduced from cDNA. Similarity of cell attachment sites in vitronectin and fibronectin. EMBO J. 1985;4:2519-24 pubmed
    ..It showed that vitronectin contains the entire 44-amino acid somatomedin B peptide at its NH2 terminus and, near its COOH terminus, a 34-amino acid glycosaminoglycan binding site in ..
  6. Suzuki S, Pierschbacher M, Hayman E, Nguyen K, Ohgren Y, Ruoslahti E. Domain structure of vitronectin. Alignment of active sites. J Biol Chem. 1984;259:15307-14 pubmed
    ..of the intact vitronectin polypeptides are the same and identical to a 44-amino acid serum peptide called somatomedin B, indicating that vitronectin may be the precursor of somatomedin B...
  7. Deng G, Curriden S, Wang S, Rosenberg S, Loskutoff D. Is plasminogen activator inhibitor-1 the molecular switch that governs urokinase receptor-mediated cell adhesion and release?. J Cell Biol. 1996;134:1563-71 pubmed
    ..First, we demonstrate that uPAR and PAI-1 bind to the same site in VN (i.e., the amino-terminal somatomedin B domain; SMB), and that PAI-1 competes with uPAR for binding to SMB...
  8. Ozaki S, Johnson L, Mullins R, Hageman G, Anderson D. The human retina and retinal pigment epithelium are abundant sources of vitronectin mRNA. Biochem Biophys Res Commun. 1999;258:524-9 pubmed
    b>Vitronectin (Vn), a multifunctional plasma protein synthesized primarily in the liver, is often present as a component of the extracellular plaques and deposits that accompany various age-related human diseases...
  9. Anderson D, Hageman G, Mullins R, Neitz M, Neitz J, Ozaki S, et al. Vitronectin gene expression in the adult human retina. Invest Ophthalmol Vis Sci. 1999;40:3305-15 pubmed
    To determine whether vitronectin (Vn), a plasma protein and extracellular matrix molecule that is also a prominent constituent of drusen, is synthesized by cells in the adult human retina...

More Information

Publications104 found, 100 shown here

  1. Nam T, Moralez A, Clemmons D. Vitronectin binding to IGF binding protein-5 (IGFBP-5) alters IGFBP-5 modulation of IGF-I actions. Endocrinology. 2002;143:30-6 pubmed
    IGF binding protein-5 (IGFBP-5) and vitronectin are matricellular proteins that are produced by smooth muscle cells and modulate their responsiveness to IGF-I...
  2. Stepanova V, Jerke U, Sagach V, Lindschau C, Dietz R, Haller H, et al. Urokinase-dependent human vascular smooth muscle cell adhesion requires selective vitronectin phosphorylation by ectoprotein kinase CK2. J Biol Chem. 2002;277:10265-72 pubmed
    Urokinase (uPA)- and urokinase receptor (uPAR)-dependent cell adhesion to the extracellular matrix protein vitronectin (Vn) is an important event in wound healing, tissue remodeling, immune response, and cancer...
  3. Aaboe M, Offersen B, Christensen A, Andreasen P. Vitronectin in human breast carcinomas. Biochim Biophys Acta. 2003;1638:72-82 pubmed
    We have analysed the occurrence of the extracellular glycoprotein vitronectin in carcinomas and normal tissue of human breast...
  4. Zhou A, Huntington J, Pannu N, Carrell R, Read R. How vitronectin binds PAI-1 to modulate fibrinolysis and cell migration. Nat Struct Biol. 2003;10:541-4 pubmed
    ..Here we show the crystal structure at a resolution of 2.3 A of the complex of the somatomedin B domain of vitronectin with PAI-1...
  5. Kenny H, Kaur S, Coussens L, Lengyel E. The initial steps of ovarian cancer cell metastasis are mediated by MMP-2 cleavage of vitronectin and fibronectin. J Clin Invest. 2008;118:1367-79 pubmed publisher
    ..MMP-2 enhanced peritoneal adhesion of OvCa cells through cleavage of ECM proteins fibronectin (FN) and vitronectin (Vn) into small fragments and increased binding of OvCa cells to these FN and Vn fragments and their receptors, ..
  6. Huai Q, Zhou A, Lin L, Mazar A, Parry G, Callahan J, et al. Crystal structures of two human vitronectin, urokinase and urokinase receptor complexes. Nat Struct Mol Biol. 2008;15:422-3 pubmed publisher
    ..This study reports the crystal structures of uPAR in complex with both urokinase (uPA) and vitronectin and reveal that uPA occupies the central cavity of the receptor, whereas vitronectin binds at the outer side of ..
  7. Schar C, Jensen J, Christensen A, Blouse G, Andreasen P, Peterson C. Characterization of a site on PAI-1 that binds to vitronectin outside of the somatomedin B domain. J Biol Chem. 2008;283:28487-96 pubmed publisher
    ..The interactions between the two proteins have been attributed primarily to binding of the somatomedin B (SMB) domain, which comprises the N-terminal 44 residues of vitronectin, to the flexible joint region of PAI-1,..
  8. Bergmann S, Lang A, Rohde M, Agarwal V, Rennemeier C, Grashoff C, et al. Integrin-linked kinase is required for vitronectin-mediated internalization of Streptococcus pneumoniae by host cells. J Cell Sci. 2009;122:256-67 pubmed publisher
    ..Here, we characterized the interaction of pneumococci with the adhesive glycoprotein vitronectin and the contribution of this protein to pneumococcal uptake by host cells in an integrin-dependent manner...
  9. Hallström T, Blom A, Zipfel P, Riesbeck K. Nontypeable Haemophilus influenzae protein E binds vitronectin and is important for serum resistance. J Immunol. 2009;183:2593-601 pubmed publisher
    ..The terminal pathway of the complement system is regulated by vitronectin that is a component of both plasma and the extracellular matrix...
  10. Singh B, Blom A, Unal C, Nilson B, Morgelin M, Riesbeck K. Vitronectin binds to the head region of Moraxella catarrhalis ubiquitous surface protein A2 and confers complement-inhibitory activity. Mol Microbiol. 2010;75:1426-44 pubmed publisher
    ..UspA2 also has the capacity to attract vitronectin that in turn binds C9 and hereby inhibits membrane attack complex (MAC) formation...
  11. Zhang D, Hudson A, Delostrinos C, Carmean N, Eastman R, Hicks B, et al. Dual sources of vitronectin in the human lower urinary tract: synthesis by urothelium vs. extravasation from the bloodstream. Am J Physiol Renal Physiol. 2011;300:F475-87 pubmed publisher
    b>Vitronectin (VN), secreted into the bloodstream by liver hepatocytes, is known to anchor epithelial cells to basement membranes through interactions with cell surface integrin receptors...
  12. Su Y, Jalalvand F, Morgelin M, Blom A, Singh B, Riesbeck K. Haemophilus influenzae acquires vitronectin via the ubiquitous Protein F to subvert host innate immunity. Mol Microbiol. 2013;87:1245-66 pubmed publisher
    Acquisition of the complement inhibitor vitronectin (Vn) is important for the respiratory tract pathogen nontypeable Haemophilus influenzae (NTHi) to escape complement-mediated killing...
  13. Hildebrand A, Preissner K, Müller Berghaus G, Teschemacher H. A novel beta-endorphin binding protein. Complement S protein (= vitronectin) exhibits specific non-opioid binding sites for beta-endorphin upon interaction with heparin or surfaces. J Biol Chem. 1989;264:15429-34 pubmed
    Human beta-endorphin (1-31) (beta H-endorphin) was found to specifically interact with purified complement S protein from human plasma...
  14. Jenne D, Hille A, Stanley K, Huttner W. Sulfation of two tyrosine-residues in human complement S-protein (vitronectin). Eur J Biochem. 1989;185:391-5 pubmed
    Human S-protein (vitronectin) and hemopexin, two structurally related plasma proteins of similar molecular mass and abundance, were analyzed for tyrosine sulfation...
  15. Seger D, Gechtman Z, Shaltiel S. Phosphorylation of vitronectin by casein kinase II. Identification of the sites and their promotion of cell adhesion and spreading. J Biol Chem. 1998;273:24805-13 pubmed
    The cell adhesion protein vitronectin (Vn) was previously shown to be the major target in human blood for an extracellular protein kinase A, which is released from platelets upon their physiological stimulation with thrombin and also ..
  16. Soldi R, Mitola S, Strasly M, Defilippi P, Tarone G, Bussolino F. Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. EMBO J. 1999;18:882-92 pubmed
    ..phosphorylation and mitogenicity induced by VEGF-A165 were enhanced in cells plated on the alphavbeta3 ligand, vitronectin, compared with cells plated on the alpha5beta1 ligand, fibronectin or the alpha2beta1 ligand, collagen...
  17. Underwood P, Kirkpatrick A, Mitchell S. New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J. 2002;365:57-67 pubmed
    b>Vitronectin is a plasma glycoprotein that binds to a variety of ligands...
  18. Mayasundari A, Whittemore N, Serpersu E, Peterson C. The solution structure of the N-terminal domain of human vitronectin: proximal sites that regulate fibrinolysis and cell migration. J Biol Chem. 2004;279:29359-66 pubmed
    ..structure of an N-terminal fragment comprising the first 51 amino acids from human plasma vitronectin, the somatomedin B (SMB) domain, has been determined by two-dimensional NMR approaches...
  19. Lynn G, Heller W, Mayasundari A, Minor K, Peterson C. A model for the three-dimensional structure of human plasma vitronectin from small-angle scattering measurements. Biochemistry. 2005;44:565-74 pubmed
    ..of the central domain and C-terminal heparin-binding domains of vitronectin with respect to the N-terminal somatomedin B (SMB) domain...
  20. Sa E Cunha C, Griffiths N, Virji M. Neisseria meningitidis Opc invasin binds to the sulphated tyrosines of activated vitronectin to attach to and invade human brain endothelial cells. PLoS Pathog. 2010;6:e1000911 pubmed publisher
    ..bacteria can increase human brain endothelial cell line (HBMEC) adhesion and entry by first binding to serum vitronectin and, to a lesser extent, fibronectin...
  21. Singh B, Jalalvand F, Morgelin M, Zipfel P, Blom A, Riesbeck K. Haemophilus influenzae protein E recognizes the C-terminal domain of vitronectin and modulates the membrane attack complex. Mol Microbiol. 2011;81:80-98 pubmed publisher
    ..region comprising amino acids PE 84-108 also interferes with complement-mediated bacterial killing by capturing vitronectin (Vn) that prevents complement deposition and formation of the membrane attack complex (MAC)...
  22. Weinacker A, Chen A, Agrez M, Cone R, Nishimura S, Wayner E, et al. Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor. J Biol Chem. 1994;269:6940-8 pubmed
    ..of beta 6 in these cells had the dominant negative effect of decreasing alpha v beta 5-dependent adhesion to vitronectin. In SW480 cells, beta 6 expression conferred the ability to bind to fibronectin even in the presence of ..
  23. Blouse G, Dupont D, Schar C, Jensen J, Minor K, Anagli J, et al. Interactions of plasminogen activator inhibitor-1 with vitronectin involve an extensive binding surface and induce mutual conformational rearrangements. Biochemistry. 2009;48:1723-35 pubmed publisher
    ..biphasic binding of PAI-1 to native vitronectin but only a monophasic association with the somatomedin B (SMB) domain, suggesting that multiple phases of the binding interaction occur only when full-length ..
  24. Mulligan Kehoe M, Wagner R, Wieland C, Powell R. A truncated plasminogen activator inhibitor-1 protein induces and inhibits angiostatin (kringles 1-3), a plasminogen cleavage product. J Biol Chem. 2001;276:8588-96 pubmed
    ..at the amino and carboxyl termini to eliminate the reactive center loop, part of a heparin binding site, and a vitronectin binding site...
  25. Zhu W, Li W, Yang G, Fu C, Jiang G, Hu Q. Vitronectin silencing inhibits hepatocellular carcinoma in vitro and in vivo. Future Oncol. 2015;11:251-8 pubmed publisher
    To explore if inhibition of vitronectin can be used for the treatment of hepatocellular carcinoma. RNAi technology was used to silence the expression of VTN in HepG2 and SMMC 7721 cells...
  26. Whalley D, Hruby G, Alfieri F, Kneebone A, Eade T. SpaceOAR Hydrogel in Dose-escalated Prostate Cancer Radiotherapy: Rectal Dosimetry and Late Toxicity. Clin Oncol (R Coll Radiol). 2016;28:e148-54 pubmed publisher
    ..2% versus 1.3%; V80 = 0.8% versus 5.3%; V75 = 2.2% versus 9.5%; V70 = 3.7% versus 12.3%; V65 = 5.4% versus 14.7%; V40 = 22...
  27. Li S, Hao G, Peng W, Geng X, Sun J. Expression and functional characterization of vitronectin gene from Japanese flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2017;65:9-16 pubmed publisher
    ..Protein domain analysis revealed that PoVtn possesses a conserved N-terminal somatomedin B domain followed by a conserved RGD motif and four haemopexin-like domains...
  28. Leroy Dudal J, Heyman L, Gauduchon P, Carreiras F. Adhesion of human ovarian adenocarcinoma IGROV1 cells to endothelial cells is partly mediated by the alphav integrins-vitronectin adhesive system and induces an alteration of endothelial integrity. Cell Biol Int. 2005;29:482-8 pubmed
    ..Considering the ability of ovarian carcinoma to disseminate by a haematogenous way, these in vitro events could mimic a preliminary step for carcinoma cells crossing the endothelial barrier to extravasate. ..
  29. Xu W, Zhang Z, Nfon C, Yang M. Genetic and antigenic relationship of foot-and-mouth disease virus serotype O isolates with the vaccine strain O1/BFS. Vaccine. 2018;36:3802-3808 pubmed publisher
    ..O1/BFS showed similarity with 89% of the field isolates using a virus neutralization test (VNT). The P1 region encoding the FMDV capsid was sequenced and analysed for 46 strains of FMDV/O...
  30. Alexander J, Ward S, Mendy J, Manayani D, Farness P, Avanzini J, et al. Pre-clinical evaluation of a replication-competent recombinant adenovirus serotype 4 vaccine expressing influenza H5 hemagglutinin. PLoS ONE. 2012;7:e31177 pubmed publisher
  31. Tugcu D, Devecioglu O, Unuvar A, Ekmekci H, Ekmekci O, Anak S, et al. Plasma Levels of Plasminogen Activator Inhibitor Type 1 and Vitronectin in Children With Cancer. Clin Appl Thromb Hemost. 2016;22:28-33 pubmed publisher
    ..In this study, we examined the levels of plasminogen activator inhibitor 1 (PAI-1) and vitronectin in 32 newly diagnosed pediatric patients with malignancies, determined by enzyme-linked immunosorbent assay ..
  32. Tan Y, Gamage A, Gan Y. Complement-activated vitronectin enhances the invasion of nonphagocytic cells by bacterial pathogens Burkholderia and Klebsiella. Cell Microbiol. 2017;19: pubmed publisher
    ..containing peptide and αV β3 blocking antibody identified complement-activated vitronectin as the factor facilitating this invasion...
  33. Carduner L, Agniel R, Kellouche S, Picot C, Blanc Fournier C, Leroy Dudal J, et al. Ovarian cancer ascites-derived vitronectin and fibronectin: combined purification, molecular features and effects on cell response. Biochim Biophys Acta. 2013;1830:4885-97 pubmed publisher
    ..Since fibronectin and vitronectin are key actors in ovarian cancer progression, we investigated their occurrence and molecular characteristics in ..
  34. Tas F, Karabulut S, Bilgin E, Taştekin D, Duranyildiz D. Clinical significance of serum fibronectin and vitronectin levels in melanoma patients. Melanoma Res. 2014;24:475-9 pubmed publisher
    Fibronectin and vitronectin are the important components of the extracellular matrix proteins. The aim of this study was to determine the clinical significance of these protein serum levels in patients with melanoma...
  35. Martínez de la Puente J, Ferraguti M, Ruiz S, Roiz D, Llorente F, Pérez Ramírez E, et al. Mosquito community influences West Nile virus seroprevalence in wild birds: implications for the risk of spillover into human populations. Sci Rep. 2018;8:2599 pubmed publisher
    ..WNV seroprevalence was evaluated using an epitope-blocking ELISA kit and a micro virus-neutralization test (VNT). The presence of WNV antibodies was confirmed in 1.96% and 0.67% of birds by ELISA and VNT, respectively...
  36. Horner S, Wilkins C, Badil S, Iskarpatyoti J, Gale M. Proteomic analysis of mitochondrial-associated ER membranes (MAM) during RNA virus infection reveals dynamic changes in protein and organelle trafficking. PLoS ONE. 2015;10:e0117963 pubmed publisher
  37. Liakka K, Autio Harmainen H. Distribution of the extracellular matrix proteins tenascin, fibronectin, and vitronectin in fetal, infant, and adult human spleens. J Histochem Cytochem. 1992;40:1203-10 pubmed
    ..we investigated the distribution of the extracellular matrix (ECM) glycoproteins tenascin, fibronectin, and vitronectin in fetal [16-24 gestational weeks (GW)], infant (40 GW), and adult human spleens to clarify the presence of ..
  38. Jenne D, Stanley K. Molecular cloning of S-protein, a link between complement, coagulation and cell-substrate adhesion. EMBO J. 1985;4:3153-7 pubmed
    ..Striking homology is found in the rest of the sequence with the serum spreading factor, vitronectin, which has also been shown to contain somatomedin B sequences at its amino terminus...
  39. Zheng D, Woodard A, Tallini G, Languino L. Substrate specificity of alpha(v)beta(3) integrin-mediated cell migration and phosphatidylinositol 3-kinase/AKT pathway activation. J Biol Chem. 2000;275:24565-74 pubmed
    The alpha(v)beta(3) integrin has been shown to bind several ligands, including osteopontin and vitronectin. Its role in modulating cell migration and downstream signaling pathways in response to specific extracellular matrix ligands has ..
  40. Yang M, Xu W, Bittner H, Horsington J, Vosloo W, Goolia M, et al. Generation of mAbs to foot-and-mouth disease virus serotype A and application in a competitive ELISA for serodiagnosis. Virol J. 2016;13:195 pubmed
    ..Currently antibodies to FMDV are detected in animals using the virus neutralization test (VNT) and the enzyme-linked immunosorbent assay (ELISA)...
  41. Rola J, Larska M, Socha W, Rola J, Materniak M, Urban Chmiel R, et al. Seroprevalence of bovine herpesvirus 1 related alphaherpesvirus infections in free-living and captive cervids in Poland. Vet Microbiol. 2017;204:77-83 pubmed publisher
    ..originating from 59 forest districts of Poland were tested with IBR gB ELISA and virus neutralization test (VNT) against BoHV-1 and cervid herpesvirus 1 (CvHV-1)...
  42. Sreenivasa B, Mohapatra J, Pauszek S, Koster M, Dhanya V, Tamil Selvan R, et al. Recombinant human adenovirus-5 expressing capsid proteins of Indian vaccine strains of foot-and-mouth disease virus elicits effective antibody response in cattle. Vet Microbiol. 2017;203:196-201 pubmed publisher
    ..After primary immunization, virus neutralization tests (VNT) showed seroconversion in 83, 67 and 33% of animals vaccinated with Ad5-FMD O, A and Asia 1, respectively...
  43. Cambier S, Mu D, O CONNELL D, Boylen K, Travis W, Liu W, et al. A role for the integrin alphavbeta8 in the negative regulation of epithelial cell growth. Cancer Res. 2000;60:7084-93 pubmed
    ..Finally, when tested in vivo, beta8 potently inhibited tumor growth in nude mice. Together, these results implicate alphavbeta8 as a novel growth-regulatory molecule of epithelial cells. ..
  44. Hong S, Koh Y, Chung K, Kim D. Snake venom disintegrin, saxatilin, inhibits platelet aggregation, human umbilical vein endothelial cell proliferation, and smooth muscle cell migration. Thromb Res. 2002;105:79-86 pubmed
    ..Interaction of human umbilical vein cell to immobilized vitronectin is also inhibited by binding of saxatilin to alpha(v)beta(3) integrin...
  45. Köhler S, Hallström T, Singh B, Riesbeck K, Spartà G, Zipfel P, et al. Binding of vitronectin and Factor H to Hic contributes to immune evasion of Streptococcus pneumoniae serotype 3. Thromb Haemost. 2015;113:125-42 pubmed publisher
    ..In this study, we demonstrated that Hic also interacts with vitronectin, a fluid-phase regulator involved in haemostasis, angiogenesis, and the terminal complement cascade as well as ..
  46. Ding Y, Shen Y. Notch increased vitronection adhesion protects myeloma cells from drug induced apoptosis. Biochem Biophys Res Commun. 2015;467:717-22 pubmed publisher
    ..up-regulated expression of integrin αvβ5 in myeloma cells companied with enhanced cells adhesion on vitronectin. Silencing Notch-1 receptor with siRNA or blocking cells with integrin αvβ5 antibody reduced myeloma ..
  47. Milicevic V, Radojicic S, Valcic M, Ivovic V, Radosavljevic V. Evidence of Aujeszky's disease in wild boar in Serbia. BMC Vet Res. 2016;12:134 pubmed publisher
    ..Neutralizing antibodies were not detected by virus neutralisation test (VNT) in sera from four out of eight PCR positive wild boars suggesting recent infection in those animals...
  48. Chourabi M, Liew M, Lim S, H mida Ben Brahim D, Boussofara L, Dai L, et al. ENPP1 Mutation Causes Recessive Cole Disease by Altering Melanogenesis. J Invest Dermatol. 2018;138:291-300 pubmed publisher
    ..Cys120Arg allele represents a recessive and more severe form of Cole disease. ..
  49. Schvartz I, Seger D, Maik Rachline G, Kreizman T, Shaltiel S. Truncated vitronectins: binding to immobilized fibrin and to fibrin clots, and their subsequent interaction with cells. Biochem Biophys Res Commun. 2002;290:682-9 pubmed
    The plasminogen activator inhibitor-1 (PAI-1) is stabilized in its inhibitory conformation by binding to Vitronectin (Vn)...
  50. Bornstein S, Schmidt M, Choonoo G, Levin T, Gray J, Thomas C, et al. IL-10 and integrin signaling pathways are associated with head and neck cancer progression. BMC Genomics. 2016;17:38 pubmed publisher
    ..Mutational analysis highlighted a potential role for CTCF, a crucial regulator of long-range chromatin interactions, in head and neck cancer progression. ..
  51. Murgić J, Chung P, Berlin A, Bayley A, Warde P, Catton C, et al. Lessons learned using an MRI-only workflow during high-dose-rate brachytherapy for prostate cancer. Brachytherapy. 2016;15:147-55 pubmed publisher
    ..Conventional dose-planning objectives for the rectum (V75 < 1...
  52. Smith J, Vestal D, Irwin S, Burke T, Cheresh D. Purification and functional characterization of integrin alpha v beta 5. An adhesion receptor for vitronectin. J Biol Chem. 1990;265:11008-13 pubmed
    We have purified a novel member of the integrin gene family from placenta that serves as a vitronectin receptor. This integrin is composed of the alpha v subunit and a beta subunit that we designate beta 5...
  53. Chavakis T, Kanse S, Lupu F, Hammes H, Muller Esterl W, Pixley R, et al. Different mechanisms define the antiadhesive function of high molecular weight kininogen in integrin- and urokinase receptor-dependent interactions. Blood. 2000;96:514-22 pubmed
    ..arginyl-glycyl-aspartyl peptide, indicating that HKa interacts with the amino terminal portion of VN ("somatomedin B region")...
  54. Otsuka A, Hirose K, Kilimann M, Kamata T. Amphiphysin1 inhibits vitronectin-mediated cell adhesion, spreading, and migration in vitro. Biochem Biophys Res Commun. 2003;301:769-75 pubmed
    ..b>Vitronectin, a major cell-adhesive protein in serum and a ligand to alpha(v)beta3 integrin, was responsible for this cell ..
  55. Hollier B, Harkin D, Leavesley D, Upton Z. Responses of keratinocytes to substrate-bound vitronectin: growth factor complexes. Exp Cell Res. 2005;305:221-32 pubmed
    Insulin-like growth factor-1 (IGF-I) can associate with the extracellular matrix protein vitronectin (VN) via select IGF-binding proteins, and the resulting complex stimulates responses in a variety of cell types...
  56. Kohler T, Gisch N, Binsker U, Schlag M, Darm K, Volker U, et al. Repeating structures of the major staphylococcal autolysin are essential for the interaction with human thrombospondin 1 and vitronectin. J Biol Chem. 2014;289:4070-82 pubmed publisher
    ..Atl has also been identified previously as a staphylococcal vitronectin (Vn)-binding protein...
  57. Salazar Peláez L, Abraham T, Herrera A, Correa M, Ortega J, Paré P, et al. Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease. PLoS ONE. 2015;10:e0119717 pubmed publisher
    b>Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling...
  58. Gründel A, Jacobs E, Dumke R. Interactions of surface-displayed glycolytic enzymes of Mycoplasma pneumoniae with components of the human extracellular matrix. Int J Med Microbiol. 2016;306:675-685 pubmed publisher
    ..human extracellular matrix (ECM) proteins fibrinogen (Fn), fibronectin (Fc), lactoferrin (Lf), laminin (Ln) and vitronectin (Vc), respectively...
  59. Tran A, Zhang J, Woods K, Yu V, Nguyen D, Gustafson G, et al. Treatment planning comparison of IMPT, VMAT and 4? radiotherapy for prostate cases. Radiat Oncol. 2017;12:10 pubmed publisher
    ..CTV doses, bladder and rectum dose volumes (V40, V45, V60, V65, V70, V75, and V80), R100, R50, R10, and CTV homogeneity index (D95/D5) were evaluated...
  60. Alessi M, Nicaud V, Scroyen I, Lange C, Saut N, Fumeron F, et al. Association of vitronectin and plasminogen activator inhibitor-1 levels with the risk of metabolic syndrome and type 2 diabetes mellitus. Results from the D.E.S.I.R. prospective cohort. Thromb Haemost. 2011;106:416-22 pubmed publisher
    It was the objective of this study to investigate the relation between vitronectin and plasminogen activator inhibitor (PAI)-1 plasma levels with nine-year incidences of the metabolic syndrome (MetS) and of type 2 diabetes mellitus (T2DM)..
  61. Shen J, Ma X, Yi F, Rasul A, Cui M, Ma T. Increased expression levels of vitronectin in the maternal?fetal interface of placenta in early-onset severe preeclampsia. Mol Med Rep. 2013;7:53-8 pubmed publisher
    ..The present study was designed to analyze whether vitronectin (VN), a multifunctional human glycoprotein, is expressed in PE and normal placentas...
  62. Martí Pàmies I, Cañes L, Alonso J, Rodríguez C, Martínez González J. The nuclear receptor NOR-1/NR4A3 regulates the multifunctional glycoprotein vitronectin in human vascular smooth muscle cells. FASEB J. 2017;31:4588-4599 pubmed publisher
    ..NOR-1 response elements in the proximal promoter region of several genes encoding for ECM proteins, including vitronectin (VTN)...
  63. Lange R, Peng X, Wimmer E, Lipp M, Bernhardt G. The poliovirus receptor CD155 mediates cell-to-matrix contacts by specifically binding to vitronectin. Virology. 2001;285:218-27 pubmed
    ..Here we provide evidence that CD155 binds specifically to vitronectin with a dissociation constant (K(d)) of 72 nM as determined by surface plasmon resonance...
  64. Rahman S, Patel Y, Murray J, Patel K, Sumathipala R, Sobel M, et al. Novel hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met-integrin induced signalling pathway in endothelial cells. BMC Cell Biol. 2005;6:8 pubmed
    ..Here we report that fibronectin (FN) and vitronectin (VN) modulate the responses of endothelial cells to HGF (Scatter Factor), an important pro-angiogenic mediator...
  65. Seiffert D, Loskutoff D. Evidence that type 1 plasminogen activator inhibitor binds to the somatomedin B domain of vitronectin. J Biol Chem. 1991;266:2824-30 pubmed
    ..that did not interact with PAI-1, and a Mr 6,000 NH2-terminal fragment (Asp1-Met51) that spanned the somatomedin B domain and contained the RGD (cell binding) sequence...
  66. Chain D, Korc Grodzicki B, Kreizman T, Shaltiel S. Endogenous cleavage of the Arg-379-Ala-380 bond in vitronectin results in a distinct conformational change which 'buries' Ser-378, its site of phosphorylation by protein kinase A. Biochem J. 1991;274 ( Pt 2):387-94 pubmed
    ..release protein kinase A, which in human plasma singles out and phosphorylates one protein, identified as vitronectin. This protein is known to be involved in processes that follow platelet stimulation, specifically, in the ..
  67. Sigurdardottir O, Wiman B. Identification of a PAI-1 binding site in vitronectin. Biochim Biophys Acta. 1994;1208:104-10 pubmed
    Active PAI-1 (plasminogen activator inhibitor 1) is bound to vitronectin in plasma and in the extracellular matrix. In this study we aimed at identifying the PAI-1 binding site in vitronectin, which at present is a matter of dispute...
  68. Upton Z, Webb H, Hale K, Yandell C, McMurtry J, Francis G, et al. Identification of vitronectin as a novel insulin-like growth factor-II binding protein. Endocrinology. 1999;140:2928-31 pubmed
    ..amino acid sequencing of the purified protein revealed that it has the same N-terminal sequence as chicken vitronectin (VN)...
  69. Schroeck F, Arroyo De Prada N, Sperl S, Schmitt M, Viktor M. Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin (Vn): mapping the binding sites on PAI-1 and Vn. Biol Chem. 2002;383:1143-9 pubmed
    ..The major high-affinity PAI-1 binding region of Vn is localized within the N-terminal somatomedin B (SMB) domain of Vn...
  70. Hyde C, Hollier B, Anderson A, Harkin D, Upton Z. Insulin-like growth factors (IGF) and IGF-binding proteins bound to vitronectin enhance keratinocyte protein synthesis and migration. J Invest Dermatol. 2004;122:1198-206 pubmed
    ..Additionally, it's been demonstrated that IGF-II binds directly to the extracellular matrix protein vitronectin (VN), whereas IGF-I does not...
  71. Hallström T, Trajkovska E, Forsgren A, Riesbeck K. Haemophilus influenzae surface fibrils contribute to serum resistance by interacting with vitronectin. J Immunol. 2006;177:430-6 pubmed
    b>Vitronectin inhibits the membrane attack complex of the complement system and is found both in plasma and the extracellular matrix...
  72. Kundu A, Putnam A. Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells. Biochem Biophys Res Commun. 2006;347:347-57 pubmed
    ..To investigate this question, we cultured MSCs on purified vitronectin or type-I collagen, motivated by our earlier tissue engineering work demonstrating that MSC adhesion to polymer ..
  73. Upton Z, Cuttle L, Noble A, Kempf M, Topping G, Malda J, et al. Vitronectin: growth factor complexes hold potential as a wound therapy approach. J Invest Dermatol. 2008;128:1535-44 pubmed publisher
    ..We report herein that complexes comprising IGF and IGF-binding proteins bound to the ECM protein vitronectin (VN) significantly enhance cellular functions relevant to wound repair in human skin keratinocytes in two- and ..
  74. Somanath P, Malinin N, Byzova T. Cooperation between integrin alphavbeta3 and VEGFR2 in angiogenesis. Angiogenesis. 2009;12:177-85 pubmed publisher
    ..The pathological consequences and regulatory events involved in this receptor cross-talk are also presented. ..
  75. Hurt E, Chan K, Serrat M, Thomas S, Veenstra T, Farrar W. Identification of vitronectin as an extrinsic inducer of cancer stem cell differentiation and tumor formation. Stem Cells. 2010;28:390-8 pubmed publisher
    ..Here we show that vitronectin is the component of human serum driving stem cell differentiation through an integrin alpha V beta 3-dependent ..
  76. Kellouche S, Fernandes J, Leroy Dudal J, Gallet O, Dutoit S, Poulain L, et al. Initial formation of IGROV1 ovarian cancer multicellular aggregates involves vitronectin. Tumour Biol. 2010;31:129-39 pubmed publisher
    ..We have previously demonstrated the involvement of the vitronectin/alphav integrin adhesive system in the dissemination of ovarian cancer cells and continue to investigate the ..
  77. Griffiths N, Hill D, Borodina E, Sessions R, Devos N, Feron C, et al. Meningococcal surface fibril (Msf) binds to activated vitronectin and inhibits the terminal complement pathway to increase serum resistance. Mol Microbiol. 2011;82:1129-49 pubmed publisher
    ..Previously, we have shown that Nm Opc binds to serum vitronectin to inhibit complement-mediated killing...
  78. Pirazzoli V, Ferraris G, Sidenius N. Direct evidence of the importance of vitronectin and its interaction with the urokinase receptor in tumor growth. Blood. 2013;121:2316-23 pubmed publisher
    ..studies have substantiated the importance of the interaction between uPAR and the extracellular matrix protein vitronectin (VN) for the signaling activity of the receptor in vitro, however, the possible relevance of this interaction ..
  79. Aslan S, Ikitimur B, Cakmak H, Karadag B, Tufekcioglu E, Ekmekci H, et al. Prognostic utility of serum vitronectin levels in acute myocardial infarction. Herz. 2015;40:685-9 pubmed publisher
    b>Vitronectin (VN) functions as a regulator of platelet adhesion and aggregation, coagulation, and fibrinolysis...
  80. Hwang H, Lee J, Lee H, Park G, Jeong H, Moon M, et al. In-depth analysis of site-specific N-glycosylation in vitronectin from human plasma by tandem mass spectrometry with immunoprecipitation. Anal Bioanal Chem. 2014;406:7999-8011 pubmed publisher
    ..b>Vitronectin is well known to be a multifunctional glycoprotein in the blood and the extracellular matrix, which is related ..
  81. Yang X, Zhou L, Yang Q, Liu L, Cai Y, Ma S. Diagnostic and prognostic roles of serum vitronectin in hepatitis B-related hepatocellular carcinoma. Cancer Biomark. 2016;17:271-279 pubmed
    b>Vitronectin (VN) might be involved in the progression of hepatocellular carcinoma (HCC). This study was designed to evaluate the diagnostic and prognostic value of serum vitronectin among HCC patients...
  82. Amer S, Kadi A, Darwish H, Attwa M. Liquid chromatography tandem mass spectrometry method for the quantification of vandetanib in human plasma and rat liver microsomes matrices: metabolic stability investigation. Chem Cent J. 2017;11:45 pubmed publisher
    Vandetanib (VNT) is a new oral tyrosine kinase inhibitor that acts mainly by inhibiting vascular endothelial growth factor receptor (VEGFR)...
  83. Keasey M, Jia C, Pimentel L, Sante R, Lovins C, Hagg T. Blood vitronectin is a major activator of LIF and IL-6 in the brain through integrin-FAK and uPAR signaling. J Cell Sci. 2018;131: pubmed publisher
    We defined how blood-derived vitronectin (VTN) rapidly and potently activates leukemia inhibitory factor (LIF) and pro-inflammatory interleukin 6 (IL-6) in vitro and after vascular injury in the brain...
  84. Hagege J, Peraldi M, Rondeau E, Adida C, Delarue F, Medcalf R, et al. Plasminogen activator inhibitor-1 deposition in the extracellular matrix of cultured human mesangial cells. Am J Pathol. 1992;141:117-28 pubmed
    ..This can explain the absence of detectable PAI-1 in normal human kidney biopsies. t-PA released by mesangial cells can bind and detach matrix PAI-1. ..
  85. Jenne D, Stanley K. Nucleotide sequence and organization of the human S-protein gene: repeating peptide motifs in the "pexin" family and a model for their evolution. Biochemistry. 1987;26:6735-42 pubmed
    ..glycine residue within repeating peptide segments, whereas introns 1 and 2 are at the boundaries of the Somatomedin B domain of S-protein...
  86. Nuovo G, Preissner K, Bronson R. PCR-amplified vitronectin mRNA localizes in situ to spermatocytes and round spermatids in the human testis. Hum Reprod. 1995;10:2187-91 pubmed
    b>Vitronectin is an adhesion protein present within the acrosomal cap region of human spermatozoa and is liberated during the acrosome reaction...
  87. Gechtman Z, Shaltiel S. Phosphorylation of vitronectin on Ser362 by protein kinase C attenuates its cleavage by plasmin. Eur J Biochem. 1997;243:493-501 pubmed
    b>Vitronectin, found in the extracellular matrix and in circulating blood, has an important role in the control of plasminogen activation...
  88. Lawrence D, Palaniappan S, Stefansson S, Olson S, Francis Chmura A, Shore J, et al. Characterization of the binding of different conformational forms of plasminogen activator inhibitor-1 to vitronectin. Implications for the regulation of pericellular proteolysis. J Biol Chem. 1997;272:7676-80 pubmed
    ..the primary physiologic inhibitor of plasminogen activation, is associated with the adhesive glycoprotein vitronectin (Vn) in plasma and the extracellular matrix...
  89. Memmo L, McKeown Longo P. The alphavbeta5 integrin functions as an endocytic receptor for vitronectin. J Cell Sci. 1998;111 ( Pt 4):425-33 pubmed
    Endocytosis and degradation of vitronectin by human skin fibroblasts are regulated by the beta5 integrin...
  90. Gil J, Woo D, Shim J, Kim S, You H, Park S, et al. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett. 2009;583:561-7 pubmed publisher
    ..during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord...
  91. Kadowaki M, Sangai T, Nagashima T, Sakakibara M, Yoshitomi H, Takano S, et al. Identification of vitronectin as a novel serum marker for early breast cancer detection using a new proteomic approach. J Cancer Res Clin Oncol. 2011;137:1105-15 pubmed publisher
    ..Western blotting confirmed that the level of vitronectin was significantly increased in DCIS patients compared with that of normal controls...