VDAC1

Summary

Gene Symbol: VDAC1
Description: voltage-dependent anion channel 1
Alias: PORIN, VDAC-1, outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, porin 31HM, voltage-dependent anion-selective channel protein 1
Species: human

Top Publications

  1. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
  2. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
  3. doi Outer membrane VDAC1 controls permeability transition of the inner mitochondrial membrane in cellulo during stress-induced apoptosis
    Flora Tomasello
    INSERM U916, Universite Bordeaux 2, Institut Bergonie, 33076 Bordeaux, France
    Cell Res 19:1363-76. 2009
  4. ncbi Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells
    Changjiang Weng
    Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China 100080
    J Biol Chem 280:10491-500. 2005
  5. pmc Solution structure of the integral human membrane protein VDAC-1 in detergent micelles
    Sebastian Hiller
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Science 321:1206-10. 2008
  6. doi Voltage-dependent anion channel 1 is involved in endostatin-induced endothelial cell apoptosis
    Shaopeng Yuan
    Beijing Key Laboratory for Protein Therapeutics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    FASEB J 22:2809-20. 2008
  7. doi Voltage-dependent anion channel 1-based peptides interact with hexokinase to prevent its anti-apoptotic activity
    Laetitia Arzoine
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel 84105
    J Biol Chem 284:3946-55. 2009
  8. doi Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease
    Tamami Kikuchi
    Department of Cellular Physiology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Niigata, 951 8510, Japan
    Mod Rheumatol 18:570-7. 2008
  9. ncbi Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivo
    Inbar Koren
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cancer Biol Ther 9:1046-52. 2010
  10. ncbi [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)]
    H Kayser
    Max Planck Institut fur experimentelle Medizin, Abteilung Immunchemie, Gottingen
    Biol Chem Hoppe Seyler 370:1265-78. 1989

Research Grants

  1. Study of Mechanisms Leading to Multidrug-Resistant K. pneumoniae and A. baumannii
    John Quale; Fiscal Year: 2007
  2. Neisserial Porins and Antigen Presenting Cells
    LEE MARK WETZLER; Fiscal Year: 2010
  3. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
  4. REGULATION OF THE HEAT SHOCK RESPONSE IN E. COLI
    CAROL ANNE GROSS; Fiscal Year: 2010
  5. REGULATION OF THE HEAT SHOCK RESPONSE IN E. COLI
    Carol Gross; Fiscal Year: 2009
  6. MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINS
    ANNE DELCOUR; Fiscal Year: 2003
  7. HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUM
    THOMAS PISTOLE; Fiscal Year: 2003
  8. Neisserial Porins and Antigen Presenting Cells
    Lee Wetzler; Fiscal Year: 2005
  9. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2007
  10. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2009

Detail Information

Publications193 found, 100 shown here

  1. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
    ..Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores...
  2. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..In agreement with this, VDAC1-deficient mitochondria from a mutant yeast did not exhibit a Bax/Bak-induced loss in membrane potential and ..
  3. doi Outer membrane VDAC1 controls permeability transition of the inner mitochondrial membrane in cellulo during stress-induced apoptosis
    Flora Tomasello
    INSERM U916, Universite Bordeaux 2, Institut Bergonie, 33076 Bordeaux, France
    Cell Res 19:1363-76. 2009
    ..Working at the single live cell level, we found that overexpression of VDAC1 triggers MPT at the mitochondrial inner membrane (MIM)...
  4. ncbi Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells
    Changjiang Weng
    Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China 100080
    J Biol Chem 280:10491-500. 2005
    ..These results suggested that removal of N-terminal domains of Bid by caspase-8 and Mcl-1 by caspase-3 enabled the maximal mitochondrial perturbation that potentiated TRAIL-induced apoptosis...
  5. pmc Solution structure of the integral human membrane protein VDAC-1 in detergent micelles
    Sebastian Hiller
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Science 321:1206-10. 2008
    ..NMR measurements revealed the binding sites of VDAC-1 for the Bcl-2 protein Bcl-x(L), for reduced beta-nicotinamide adenine dinucleotide, and for cholesterol. Bcl-x(L) interacts with the VDAC barrel laterally at strands 17 and 18...
  6. doi Voltage-dependent anion channel 1 is involved in endostatin-induced endothelial cell apoptosis
    Shaopeng Yuan
    Beijing Key Laboratory for Protein Therapeutics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    FASEB J 22:2809-20. 2008
    ..ES promotes the mitochondrial permeability transition pore (mPTP) opening via voltage-dependent anion channel 1 (VDAC1), a major component of mitochondrial outer membrane...
  7. doi Voltage-dependent anion channel 1-based peptides interact with hexokinase to prevent its anti-apoptotic activity
    Laetitia Arzoine
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel 84105
    J Biol Chem 284:3946-55. 2009
    ..Now, we demonstrate that synthetic peptides corresponding to the VDAC1 N-terminal region and selected sequences bound specifically, in a concentration- and time-dependent manner, to ..
  8. doi Human voltage-dependent anion selective channel 1 is a target antigen for antiglomerular endothelial cell antibody in mixed connective tissue disease
    Tamami Kikuchi
    Department of Cellular Physiology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Niigata, 951 8510, Japan
    Mod Rheumatol 18:570-7. 2008
    ....
  9. ncbi Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivo
    Inbar Koren
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cancer Biol Ther 9:1046-52. 2010
    The voltage-dependent anion channel 1 (VDAC1), located at the outer mitochondrial membrane (OMM), is a key protein in regulating the exchange of ions, nucleotides and a variety of metabolites in and out of the mitochondria...
  10. ncbi [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)]
    H Kayser
    Max Planck Institut fur experimentelle Medizin, Abteilung Immunchemie, Gottingen
    Biol Chem Hoppe Seyler 370:1265-78. 1989
    ..structure of a human porin with the molecular mass of 31 kDa derived from the plasmalemm of B-lymphocytes (Porin 31HL). Porin 31HL is shown to be a basic, channel forming membrane protein...
  11. pmc Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, CREST of Japan Science and Technology Corporation JST, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:577-82. 2000
    ..These findings indicated the involvement of a different mechanism in BH3-only, protein-induced apoptogenic cytochrome c release...
  12. ncbi Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74
    Christian Schwarzer
    Max Planck Institute for Experimental Medicine, Department of Immunochemistry, Hermann Rein Street 3, 37075 Gottingen, Germany
    Int J Biochem Cell Biol 34:1059-70. 2002
    The voltage-dependent anion-selective channel 1 (VDAC1), i.e...
  13. pmc Control of mitochondrial membrane permeabilization by adenine nucleotide translocator interacting with HIV-1 viral protein rR and Bcl-2
    E Jacotot
    Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, F 94805 Villejuif, France
    J Exp Med 193:509-19. 2001
    ..Concomitantly, Bcl-2 suppresses channel formation by the ANT-Vpr complex in synthetic membranes. In conclusion, both Vpr and Bcl-2 modulate MMP through a direct interaction with ANT...
  14. pmc Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier
    M W McEnery
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185
    Proc Natl Acad Sci U S A 89:3170-4. 1992
    ..VDAC and ADC, outer and inner mitochondrial membrane channel proteins, respectively, together with the 18-kDa subunit, may comprise mBzR at functionally important transport sites at the junction of two mitochondrial membranes...
  15. pmc BH4 domain of antiapoptotic Bcl-2 family members closes voltage-dependent anion channel and inhibits apoptotic mitochondrial changes and cell death
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, and Core Research for Evolutional Science and Technology CREST of Japan Science and Technology Corporation, 2 2 Yamadaoka, Suita
    Proc Natl Acad Sci U S A 97:3100-5. 2000
    ..Finally, the data suggest that the TAT-BH4 peptide is potentially useful as a therapeutic agent for diseases caused by accelerated apoptosis...
  16. ncbi Cyclophilin-D binds strongly to complexes of the voltage-dependent anion channel and the adenine nucleotide translocase to form the permeability transition pore
    M Crompton
    Department of Biochemistry and Molecular Biology, University College London, UK
    Eur J Biochem 258:729-35. 1998
    ..band on SDS/PAGE resolved into a doublet and reacted with antibodies against the voltage-dependent anion channel (porin) and the adenine nucleotide translocase...
  17. ncbi Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel
    E Blachly-Dyson
    Vollum Institute for Advanced Biomedical Research, Portland, Oregon
    J Biol Chem 268:1835-41. 1993
    ..The expression of each human cDNA has been assessed by Northern blot and polymerase chain reaction techniques. With one exception, each is expressed in all human cell lines and tissues examined...
  18. pmc Paraquat toxicity induced by voltage-dependent anion channel 1 acts as an NADH-dependent oxidoreductase
    Hiroki Shimada
    Molecular and Cell Structural Science, Kanazawa Medical University, Uchinada, Ishikawa 920 0293, Japan
    J Biol Chem 284:28642-9. 2009
    ..we demonstrated that mitochondrial NADH-dependent PQ reductase containing a voltage-dependent anion channel 1 (VDAC1) is responsible for PQ cytotoxicity...
  19. doi Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of binding
    Salah Abu-Hamad
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 283:13482-90. 2008
    ..We have previously shown that HK-I decreases murine VDAC1 (mVDAC1) channel conductance, inhibits cytochrome c release, and protects against apoptotic cell death...
  20. pmc The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore
    E Jacotot
    Centre National de la Recherche Scientifique, F 94801 Villejuif, France
    J Exp Med 191:33-46. 2000
    ..Hence, Vpr induces apoptosis via a direct effect on the mitochondrial PTPC...
  21. pmc Neisseria meningitidis porin PorB interacts with mitochondria and protects cells from apoptosis
    P Massari
    Evans Biomedical Research Center, 650 Albany Street, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 97:9070-5. 2000
    Neisserial porins are strong immune adjuvants and B cell activators. The effect of neisserial porin PorB on activation-induced cell death was investigated, as a potential additional mechanism of the porin's immunopotentiating ability...
  22. ncbi Functional characterization of the conserved "GLK" motif in mitochondrial porin from Neurospora crassa
    G Runke
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    J Bioenerg Biomembr 32:563-70. 2000
    Mitochondrial porin facilitates the diffusion of small hydrophilic molecules across the mitochondrial outer membrane...
  23. ncbi [The mechanism of antigen-presentation of porin from Salmonella typhimurium to T lymphocytes]
    K Matsui
    Department of Microbiology, Meiji College of Pharmacy
    Kansenshogaku Zasshi 68:491-4. 1994
    ..It is understood that these three species of proteins construct porin trimers and the porin trimers from the wild type strain of S...
  24. ncbi Modulation of the voltage-dependent anion channel (VDAC) by glutamate
    D Gincel
    Department of Life Sciences, The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel
    J Bioenerg Biomembr 32:571-83. 2000
    The voltage-dependent anion channel (VDAC), also known as mitochondrial porin, is a large channel permeable to anions, cations, ATP, and other metabolites...
  25. ncbi Molecular and cellular mechanisms used in the acute phase of stimulated steroidogenesis
    M Thomson
    Department of Biological Sciences, University of Western Sydney Nepean, Kingswood, Australia
    Horm Metab Res 30:16-28. 1998
    ..These contact sites may be composed of protein bridges which include the constituents, porin, the benzodiazepine receptor and GTP binding proteins...
  26. ncbi [Alteration of serotype and drug susceptibility of some Pseudomonas aeruginosa isolates by exposure to human serum and polymorphonuclear leukocytes]
    I Kobayashi
    Chemotherapy Division, Mitsubishi Yuka Bio Clinical Laboratories, Inc. Tokyo
    Kansenshogaku Zasshi 68:500-7. 1994
    ..susceptibilities to some beta-lactams and norfloxacin which could penetrate into the bacterial cells through the porin channels of the outer membrane...
  27. ncbi Binding of rat brain hexokinase to recombinant yeast mitochondria: identification of necessary molecular determinants
    H Azoulay-Zohar
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Bioenerg Biomembr 31:569-79. 1999
    ..brain hexokinase to mitochondria from rat liver or yeast (wild type, porinless, or expressing recombinant human porin) was studied in an effort to identify minimal requirements for each component...
  28. ncbi Diffusion of beta-lactam antibiotics through oligomeric or monomeric porin channels of some gram-negative bacteria
    Yeshayahu Nitzan
    Health Sciences Research Center, Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Curr Microbiol 45:446-55. 2002
    ..The major proteins with porin activity were purified from the outer membranes of six bacteria. Three of the six porins were oligomeric porins...
  29. ncbi Homology models of the Yersinia pseudotuberculosis and Yersinia pestis general porins and comparative analysis of their functional and antigenic regions
    G N Likhatskaya
    Pacific Institute of Bioorganic Chemistry of Far East Branch of Russian Academy of Sciences, Vladivostok, Pr 100 Let Vladivostoku 159, 690022 Russia
    J Biomol Struct Dyn 23:163-74. 2005
    The amino acid sequences of the Yersinia pseudotuberculosis porin (YPS) and Y. pestis porin (YPT) have recently deduced but their three-dimensional structures were not known...
  30. pmc Energy-dependent accumulation of norfloxacin and porin expression in clinical isolates of Klebsiella pneumoniae and relationship to extended-spectrum beta-lactamase production
    Luis Martinez-Martinez
    Department of Microbiology, School of Medicine, University of Seville, Spain
    Antimicrob Agents Chemother 46:3926-32. 2002
    The relationships between porin deficiency, active efflux of fluoroquinolones, and extended-spectrum beta-lactamase (ESBL) production were determined for 53 clinical isolates of Klebsiella pneumoniae...
  31. ncbi Characterization of further association of the trimeric membrane protein porin by low-angle laser-light scattering photometry coupled with high-performance gel chromatography
    Y Watanabe
    Institute for Protein Research, Osaka University, Japan
    J Chromatogr A 653:241-6. 1993
    b>Porin (OmpF), a trimeric membrane protein, in an extract of the outer membrane of Escherichia coli gave a twin-peaked elution pattern on Sephacryl S-300HR gel chromatography in the presence of sodium dodecyl sulphate...
  32. pmc X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: implications for ion selectivity and single-channel conductance
    M Przybylski
    Universitat Konstanz, Fakultat fur Chemie, Germany
    Protein Sci 5:1477-89. 1996
    ..To introduce new negative charges in a channel protein, amino groups of porin from Rhodobacter capsulatus 37b4 were succinylated with succinic anhydride, and the precise extent and sites of ..
  33. pmc Porins from Salmonella enterica serovar Typhimurium activate the transcription factors activating protein 1 and NF-kappaB through the Raf-1-mitogen-activated protein kinase cascade
    Massimiliano Galdiero
    Istituto di Microbiologia, Facolta di Medicina e Chirurgia, Seconda Universita degli Studi di Napoli, 80138 Naples, Italy
    Infect Immun 70:558-68. 2002
    ..that these enzymes may participate in cell signaling pathways leading to AP-1 and NF-kappaB activation following porin stimulation of cells...
  34. ncbi Identification of positively charged residues of FomA porin of Fusobacterium nucleatum which are important for pore function
    H Kleivdal
    Department Molecular Biology, Unviversity of Bergen, Norway
    Eur J Biochem 260:818-24. 1999
    FomA porin is the major outer-membrane protein of Fusobacterium nucleatum. It exhibits the functional properties of a general diffusion porin, but has no sequence similarity to other porins...
  35. pmc Molecular, antigenic, and functional analyses of Omp2b porin size variants of Brucella spp
    J Y Paquet
    Unité de Recherche en Biologie Moléculaire URBM, Laboratoire d Immunologie Microbiologie, Facultes Universitaires Notre Dame de la Paix, 5000 Namur, Belgium
    J Bacteriol 183:4839-47. 2001
    ..The variation of the Omp2a and Omp2b porin sequences among the various strains of the genus Brucella seems to result mostly from multiple gene conversions ..
  36. pmc Roles of beta-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae
    L Martinez-Martinez
    Department of Microbiology, School of Medicine, University of Seville, Seville, Spain
    Antimicrob Agents Chemother 43:1669-73. 1999
    ..The contribution of porin deficiency was investigated by restoring a functional ompK36 gene on a plasmid...
  37. pmc How beta-lactam antibiotics enter bacteria: a dialogue with the porins
    Chloe E James
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, IFR 88, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e5453. 2009
    ..Clinical reports have linked the MDR phenotype to altered membrane permeability including porin modification and efflux pump expression.
  38. doi A putative alpha-helical porin from Corynebacterium glutamicum
    Karin Ziegler
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstr 21, 79104 Freiburg im Breisgau, Germany
    J Mol Biol 379:482-91. 2008
    ..The exchange of material across this barrier requires porins. Porin B (PorB) is one of them...
  39. pmc Role of porins for uptake of antibiotics by Mycobacterium smegmatis
    Olga Danilchanka
    Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
    Antimicrob Agents Chemother 52:3127-34. 2008
    ..Here, we examined which antibiotics utilize the porin pathway across the outer membrane of the model organism Mycobacterium smegmatis...
  40. ncbi The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion
    I Poquet
    , Institut Pasteur, Paris, France
    Mol Microbiol 9:1061-9. 1993
    ..pullulanase variant with a serine at position +2 cofractionated mainly with the major peak of outer membrane porin. In contrast, most (55%) of a pullulanase variant with an aspartate at position +2 cofractionated with slightly ..
  41. doi Effects of ergosterol on the structure and activity of Neurospora mitochondrial porin in liposomes
    Denice C Bay
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Can J Microbiol 55:1275-83. 2009
    ..Therefore, in this work, a freeze-thaw technique was used to introduce recombinant Neurospora porin into liposomes containing, or lacking, the native fungal sterol ergosterol...
  42. ncbi The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
    C Andersen
    Lehrstuhl fur Biotechnologie, Theodor Boveri Institut Biozentrum der Universität Würzburg, Germany
    Mol Microbiol 31:499-510. 1999
    ..The resulting plasmid was transferred into the porin-deficient E. coli strain KS26 and the protein was expressed by addition of IPTG...
  43. doi Paradoxical effect of Klebsiella pneumoniae OmpK36 porin deficiency
    Xiuhong Jiang
    Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, NSW, Australia
    Pathology 41:388-92. 2009
    ..To evaluate the effect of defined mutations in the major OmpK35 and OmpK36 porins in Klebsiella pneumoniae on the activity of two common plasmid-mediated AmpC enzymes...
  44. ncbi Induction of tyrosine phosphorylated proteins in THP-1 cells by Salmonella typhimurium, Pasteurella haemolytica and Haemophilus influenzae porins
    M Galdiero
    Dipartimento di Patologia e Sanita Animale, Facolta di Veterinaria, Universita degli Studi di Napoli Federico II, Naples, Italy
    FEMS Immunol Med Microbiol 31:121-30. 2001
    ..After porin stimulation a pattern of tyrosine phosphorylated proteins appeared in the soluble cytoplasmic fraction, in the ..
  45. ncbi A novel OmpY porin from Yersinia pseudotuberculosis: structure, channel-forming activity and trimer thermal stability
    T F Solov'eva
    Pacific Institute of Bioorganic Chemistry FEBRAS, Prospect 100 Let Vladivostoku 159, Vladivostok 690022, Russia
    J Biomol Struct Dyn 28:517-33. 2011
    A novel OmpY porin was predicted based on the Yersinia pseudotuberculosis genome analysis. Whereas it has the different genomic annotation such as "outer membrane protein N" (ABS46310.1) in str...
  46. pmc Excretion of endogenous cadaverine leads to a decrease in porin-mediated outer membrane permeability
    H Samartzidou
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5513, USA
    J Bacteriol 181:791-8. 1999
    The permeability of the outer membrane of Escherichia coli to hydrophilic compounds is controlled by porin channels...
  47. ncbi Site-directed mutagenesis studies to probe the role of specific residues in the external loop (L3) of OmpF and OmpC porins in susceptibility of Serratia marcescens to antibiotics
    Sanela Begic
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Can J Microbiol 53:710-9. 2007
    ..to this natural antibiotic resistance is reduced outer membrane permeability, controlled in part by OmpF and OmpC porin proteins...
  48. ncbi Topological investigations of the FomA porin from Fusobacterium nucleatum and identification of the constriction loop L6
    H Kleivdal
    Department of Molecular Biology, University of Bergen, HIB, Thormøhlensgate 55, N 5020 Bergen, Norway
    Microbiology 147:1059-67. 2001
    b>Porin FomA in the outer membrane of Fusobacterium nucleatum is a trimeric protein, which exhibits permeability properties similar to that of the well-known enterobacterial diffusion porins...
  49. pmc The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant
    Xiuping Liu
    Evans Biomedical Research Center, Section of Infectious Diseases, Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    Vaccine 26:786-96. 2008
    ..The PorB porin from Neisseria lactamica (Nlac), a common nasopharyngeal commensal, shares significant structural and functional ..
  50. ncbi Molecular characterization of the interaction between porins of Neisseria gonorrhoeae and C4b-binding protein
    Hanna Jarva
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, University of Lund, Malmo, Sweden
    J Immunol 179:540-7. 2007
    ..The resistance of N. gonorrhoeae to normal human serum killing correlates with porin (Por)-mediated binding to the complement inhibitor, C4b-binding protein (C4BP)...
  51. pmc Putative porin of Bradyrhizobium sp. (Lupinus) bacteroids induced by glyphosate
    Nuria de María
    Instituto de Recursos Naturales, Centro de Ciencias Medioambientales, CSIC, Serrano, 115 bis, E 28006 Madrid, Spain
    Appl Environ Microbiol 73:5075-82. 2007
    ..It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a ..
  52. pmc Expression and porin activity of P28 and OMP-1F during intracellular Ehrlichia chaffeensis development
    Yumi Kumagai
    Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210 1093, USA
    J Bacteriol 190:3597-605. 2008
    ..Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as determined by a proteoliposome swelling assay...
  53. doi The influence of sterols on the conformation of recombinant mitochondrial porin in detergent
    Denice C Bay
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Biochem Cell Biol 86:539-45. 2008
    ..Nonetheless, the contributions of sterols to the folded state of mitochondrial porin are not understood...
  54. pmc Moraxella catarrhalis M35 is a general porin that is important for growth under nutrient-limiting conditions and in the nasopharynges of mice
    Donna M Easton
    Faculty of Science, University of Canberra, Canberra, Australian Capital Territory 2601, Australia
    J Bacteriol 190:7994-8002. 2008
    ..We have previously predicted the outer membrane protein M35 to be a general porin, and in the current study, we have investigated the function of M35 and its importance for survival of M...
  55. ncbi VDAC1 serves as a mitochondrial binding site for hexokinase in oxidative muscles
    Keltoum Anflous-Pharayra
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Biochim Biophys Acta 1767:136-42. 2007
    ..that mitochondria-bound hexokinase activity is significantly reduced in oxidative muscles (heart and soleus) in vdac1(-/-) mice. The activity data were supported by western blot analysis using HK2 specific antibody...
  56. ncbi Resistance to extended-spectrum beta-lactams by the emergence of SHV-12 and the loss of OmpK35 in Klebsiella pneumoniae and Escherichia coli in Malaysia
    Selvi Palasubramaniam
    Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia
    J Microbiol Immunol Infect 42:129-33. 2009
    ..This study describes the detection of SHV-12 extended-spectrum beta-lactamase (ESBL) subtype and the loss of OmpK35 porin in 4 strains of K. pneumoniae and E. coli.
  57. ncbi Functional characterization of a second porin isoform in Drosophila melanogaster. DmPorin2 forms voltage-independent cation-selective pores
    Rita Aiello
    Department of Chemical Sciences, University of Catania, 95125 Catania, Italy
    J Biol Chem 279:25364-73. 2004
    ..Genome sequencing of the fruit fly Drosophila melanogaster revealed the presence of three additional porin-like genes. No functional information was available for the different gene products...
  58. ncbi Porin isolated from the outer membrane of Erwinia amylovora and its encoding gene
    M Elazar
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
    Curr Microbiol 54:155-61. 2007
    ..The monomers of Omp-EA porin presented a topology of 16 transmembranal beta-strands...
  59. doi The role of lipids for the functional integrity of porin: an FTIR study using lipid and protein reporter groups
    Filiz Korkmaz
    Institute of Biophysics, Johann Wolfgang Goethe University, Max von Laue Strasse 1, D 60438 Frankfurt am Main, Germany
    Biochemistry 47:12126-34. 2008
    ..girdle of OmpF and the interfacial region of the lipid bilayer, leading to monomerization of this trimeric porin. The second step at 75 degrees C is attributed to the changes in lipid-porin monomer interaction...
  60. ncbi Identification of porin-like polypeptide(s) in the boundary membrane of oilseed glyoxysomes
    F J Corpas
    Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estacion Experimental del Zaidin, CSIC, Apdo 419, E 18080 Granada, Spain
    Plant Cell Physiol 41:1218-28. 2000
    ..Evidence is presented in this study that this 36-kDa polypeptide is a glyoxysomal membrane porin. A sequence of 24 amino acid residues derived from a CNBr-cleaved fragment of the 36-kDa polypeptide revealed 72% ..
  61. ncbi Distinct sensitivities of OmpF and PhoE porins to charged modulators
    H Samartzidou
    Department of Biology and Biochemistry, University of Houston, TX 77204 5513, USA
    FEBS Lett 444:65-70. 1999
    The inhibition of the anion-selective PhoE porin by ATP and of the cation-selective OmpF porin by polyamines has been previously documented...
  62. pmc Polyamines decrease Escherichia coli outer membrane permeability
    A L Dela Vega
    Department of Biology, University of Houston, Texas 77204, USA
    J Bacteriol 178:3715-21. 1996
    ..of the outer membranes of gram-negative bacteria to hydrophilic compounds is mostly due to the presence of porin channels...
  63. ncbi The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients
    Joachim Stephan
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
    Mol Microbiol 58:714-30. 2005
    ..Nutrients cross this permeability barrier by diffusion through porins. MspA is the major porin of Mycobacterium smegmatis...
  64. ncbi Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolution
    K Zeth
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Structure 8:981-92. 2000
    ..Here, we investigate the strongly anion-selective porin Omp32 from Comamonas acidovorans, which is closely homologous to the porins of pathogenic Bordetella and Neisseria ..
  65. pmc Downregulation of mitochondrial porin inhibits cell growth and alters respiratory phenotype in Trypanosoma brucei
    Ujjal K Singha
    Department of Microbial Pathogenesis and Immune Response, Meharry Medical College, Nashville, TN 37208, USA
    Eukaryot Cell 8:1418-28. 2009
    b>Porin is the most abundant outer membrane (OM) protein of mitochondria. It forms the aqueous channel on the mitochondrial OM and mediates major metabolite flux between mitochondria and cytosol...
  66. pmc Porin protein of Neisseria gonorrhoeae: cloning and gene structure
    E C Gotschlich
    Laboratory of Bacteriology and Immunology, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 84:8135-9. 1987
    The outer membrane porin molecule of Neisseria gonorrhoeae is known as protein I (PI). Among different strains of gonococci there is variability of PI, and two main classes, PIA and PIB, have been recognized...
  67. ncbi Effects of a mutation in the Drosophila porin gene encoding mitochondrial voltage-dependent anion channel protein on phototransduction
    Sunji Lee
    Graduate School of Biotechnology, Kyunghee University, Yongin si, Gyeonggi Do, Korea
    Dev Neurobiol 67:1533-45. 2007
    ..So far three distinct isoforms of VDAC (VDAC1-3) have been reported in vertebrates, but their functions remain unknown...
  68. pmc Outer membrane porin M35 of Moraxella catarrhalis mediates susceptibility to aminopenicillins
    Marion Jetter
    Institute for Infectious Diseases, University of Bern, CH 3010 Bern, Switzerland
    BMC Microbiol 9:188. 2009
    The outer membrane protein M35 is a conserved porin of type 1 strains of the respiratory pathogen Moraxella catarrhalis...
  69. pmc Characterisation of porin genes from Mycobacterium fortuitum and their impact on growth
    Soroush Sharbati
    Freie Universitat Berlin, Institute of Veterinary Biochemistry, Berlin, Germany
    BMC Microbiol 9:31. 2009
    ..Here, porin genes from M...
  70. ncbi Tuning of the porin expression under anaerobic growth conditions by his-to-Asp cross-phosphorelay through both the EnvZ-osmosensor and ArcB-anaerosensor in Escherichia coli
    M Matsubara
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Genes Cells 5:555-69. 2000
    ..We have addressed this issue, with special reference to the osmo-responsive EnvZ and anaero-responsive ArcB phosphorelay signalling pathways in E. coli...
  71. ncbi Structural architecture of an outer membrane channel as determined by electron crystallography
    B K Jap
    Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720
    Nature 350:167-70. 1991
    ..b>Porin channels have been shown in vitro to be voltage-gated...
  72. pmc Cadaverine inhibition of porin plays a role in cell survival at acidic pH
    Hrissi Samartzidou
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA
    J Bacteriol 185:13-9. 2003
    When grown at acidic pH, Escherichia coli cells secrete cadaverine, a polyamine known to inhibit porin-mediated outer membrane permeability...
  73. doi Altered pore properties and kinetic changes in mutants of the Vibrio cholerae porin OmpU
    Brian Lauman
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5001, USA
    Mol Membr Biol 25:498-505. 2008
    ..was used to characterize the pore properties of site-directed mutants in the Vibrio cholerae general diffusion porin OmpU...
  74. ncbi Crystal structure of the monomeric porin OmpG
    Gowtham V Subbarao
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 360:750-9. 2006
    ..In contrast, the OmpG porin is unique, as it appears to function as a monomer...
  75. pmc Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU
    Melissa Pagel
    Department of Biology and Biochemistry, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
    J Bacteriol 189:8593-600. 2007
    ..Here, we have analyzed the effects of mutations in the OmpU porin of Vibrio cholerae at conserved residues that are known to affect pore properties in the Escherichia coli porins ..
  76. doi Proteomic analysis of human U937 cell line activation mediated by Haemophilus influenzae type b P2 porin and its surface-exposed loop 7
    Valeria Severino
    Department of Life Science, Second University of Naples, Via Vivaldi 43, I 81100 Caserta, Italy
    J Proteome Res 9:1050-62. 2010
    ..P2 porin, one of the best-characterized porins in terms of its functional characteristics, is the most abundant OMP in Hib ..
  77. pmc The porin OmpD from nontyphoidal Salmonella is a key target for a protective B1b cell antibody response
    Cristina Gil-Cruz
    School of Immunity and Infection and Medical Research Council Centre for Immune Regulation, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Proc Natl Acad Sci U S A 106:9803-8. 2009
    ..Furthermore Ab from porin-immunized chimeras, that have B1b cells, is sufficient to impair infection...
  78. ncbi A common channel-forming motif in evolutionarily distant porins
    R A Pauptit
    Biocenter, University of Basel, Switzerland
    J Struct Biol 107:136-45. 1991
    ..packings of Escherichia coli porins are presented (phosphoporin, maltoporin, and two crystal forms of matrix porin)...
  79. ncbi The quantitation of ADP diffusion gradients across the outer membrane of heart mitochondria in the presence of macromolecules
    Frank Norbert Gellerich
    Muskellabor der Neurologischen Klinik und Poliklinik, Martin Luther Universitat, Halle Wittenberg, Julius Kühn Str 7, D 06097 Halle Saale, Halle Wittenberg, Germany
    Biochim Biophys Acta 1554:48-56. 2002
    We have previously provided evidence that diffusion of metabolites across the porin pores of mitochondrial outer membrane is hindered...
  80. pmc DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus
    T A Ficht
    Department of Veterinary Microbiology and Parasitology, Texas A and M, University, College Station 77843
    Infect Immun 57:3281-91. 1989
    ..The amino acid composition of the predicted proteins was similar to that obtained for the Brucella porin isolated by Verstreate et al. (D. R. Verstreate, M. T. Creasy, N. T. Caveney, C. L. Baldwin, M. W. Blab, and A. J...
  81. ncbi Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae
    R Dutzler
    Department of Structural Biology, Biozentrum University of Basel, CH 4056 Basel, Switzerland
    Structure 7:425-34. 1999
    ..In Escherichia coli, major nonspecific porins are matrix porin (OmpF) and osmoporin (OmpC), which show high sequence homology...
  82. ncbi The porin protein of the outer membrane of Escherichia coli: reactivity in immunoblotting, antibody-binding by the native protein, and cross-reactivity with other enteric bacteria
    A Z Henriksen
    Department of Microbiology, Faculty of Medicine, University of Trondheim, Norway
    Acta Pathol Microbiol Immunol Scand B 95:315-21. 1987
    The experimental conditions for antibody-binding by the 38.5 kD porin protein of an E. coli 055 strain in immunoblotting were investigated...
  83. ncbi The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis
    Claudia Mailaender
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr 5, D 91058 Erlangen, Germany
    Microbiology 150:853-64. 2004
    ..To examine the importance of porins in slow-growing mycobacteria, the major porin MspA of Mycobacterium smegmatis was expressed in Mycobacterium tuberculosis and Mycobacterium bovis...
  84. ncbi Molecular and structural characterization of the HMP-AB gene encoding a pore-forming protein from a clinical isolate of Acinetobacter baumannii
    Anna Gribun
    Health Sciences Research Center, Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Curr Microbiol 47:434-43. 2003
    The major outer membrane protein of Acinetobacter baumannii is the heat-modifiable protein HMP-AB, a porin with a large pore size allowing the penetration of solutes having a molecular weight of up to approximately 800 Da...
  85. pmc Efficacy of cefepime and imipenem in experimental murine pneumonia caused by porin-deficient Klebsiella pneumoniae producing CMY-2 beta-Lactamase
    Cristina Pichardo
    Department of Microbiology, School of Medicine, University of Seville, Apdo 914, 41080 Seville, Spain
    Antimicrob Agents Chemother 49:3311-6. 2005
    Previous studies have shown decreased in vitro activity of zwitterionic cephalosporins and carbapenems against porin-deficient Klebsiella pneumoniae expressing a plasmid-mediated AmpC-type beta-lactamase (PACBL)...
  86. ncbi Porin A-specific antibody avidity in patients who are convalescing from meningococcal B disease
    Clementien L Vermont
    Laboratory for Vaccine Research, Netherlands Vaccine Institute, 3720 AL Bilthoven, The Netherlands
    Pediatr Res 58:149-52. 2005
    b>Porin A (PorA), which determines the serosubtype of Neisseria meningitidis, is the main antigen of a candidate vaccine against serogroup B meningococci, which has been shown to induce high-avidity antibodies in children...
  87. ncbi Porin of Shigella dysenteriae enhances mRNA levels for Toll-like receptor 2 and MyD88, up-regulates CD80 of murine macrophage, and induces the release of interleukin-12
    Avijit Ray
    National Institute of Cholera and Enteric Diseases, P 33, C I T Road, Scheme XM, Kolkata 700 010, West Bengal, India
    FEMS Immunol Med Microbiol 39:213-9. 2003
    Sera of patients convalescing from shigellosis reacted strongly and specifically with the 38,000 Da monomer of porin of Shigella dysenteriae type 1. Since human, the only natural host of S...
  88. pmc Immunization with the recombinant PorB outer membrane protein induces a bactericidal immune response against Neisseria meningitidis
    J Claire Wright
    Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, and Southampton General Hospital, United Kingdom
    Infect Immun 70:4028-34. 2002
    ..The meningococcal porin proteins from serogroup B meningococci have been identified as candidates for inclusion in vaccines to prevent ..
  89. ncbi Altered channel properties of porins from Haemophilus influenzae: isolates from cystic fibrosis patients
    M A Arbing
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Quebec, Canada, H3A 2B4
    J Membr Biol 189:131-41. 2002
    Changes in amino-acid sequence of the unique pore-forming protein of H. influenzae (OmpP2; porin) have been associated with increased antimicrobial resistance in H. influenzae strains isolated from cystic fibrosis patients...
  90. pmc OmpR and LeuO positively regulate the Salmonella enterica serovar Typhi ompS2 porin gene
    Marcos Fernández-Mora
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62210, Mexico
    J Bacteriol 186:2909-20. 2004
    ..serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S...
  91. pmc Renaturation of recombinant Treponema pallidum rare outer membrane protein 1 into a trimeric, hydrophobic, and porin-active conformation
    H H Zhang
    Department of Microbiology, Immunology, and Molecular Genetics, School of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Bacteriol 181:7168-75. 1999
    ..observed that while native Treponema pallidum rare outer membrane protein 1 (Tromp1) is hydrophobic and has porin activity, recombinant forms of Tromp1 do not possess these properties...
  92. doi EBV up-regulates cytochrome c through VDAC1 regulations and decreases the release of cytoplasmic Ca2+ in the NPC cell line
    Xuesong Feng
    School of Chemical and Biomedical Engineering, Technological University, 62 Nanyang Drive 637459, Singapore
    Cell Biol Int 36:733-8. 2012
    ..One of the important proteins named VDAC1 (voltage-dependent anion protein 1) on the mitochondrial outer membrane controls the pro-apoptotic signals in ..
  93. ncbi A new mechanism of antibiotic resistance in Enterobacteriaceae induced by a structural modification of the major porin
    E De
    UMR 6522, CNRS, IFRMP 23, Faculte des Sciences, 76821 Mont Saint Aignan Cedex, France
    Mol Microbiol 41:189-98. 2001
    ..resistance involves a decrease in outer membrane permeability associated with changes in an as yet uncharacterized porin. We purified the major porin from the wild-type strain and a resistant strain...
  94. pmc Genes for the major structural components of Thermotogales species' togas revealed by proteomic and evolutionary analyses of OmpA and OmpB homologs
    Amanda K Petrus
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America
    PLoS ONE 7:e40236. 2012
    ..proteins have been purified and characterized in Thermotoga maritima: the anchor protein OmpA1 (or Ompα) and the porin OmpB (or Ompβ)...
  95. pmc Prostacyclin receptor-mediated ATP release from erythrocytes requires the voltage-dependent anion channel
    Meera Sridharan
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, Saint Louis, Missouri, MO 63104, USA
    Am J Physiol Heart Circ Physiol 302:H553-9. 2012
    ....
  96. pmc Affixing N-terminal α-helix to the wall of the voltage-dependent anion channel does not prevent its voltage gating
    Oscar Teijido
    Program in Physical Biology, Eunice Kennedy Shriver NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:11437-45. 2012
    ..The three-dimensional structure of VDAC1 reveals a channel formed by 19 β-strands and an N-terminal α-helix located near the midpoint of the pore...
  97. pmc Engineered oligomerization state of OmpF protein through computational design decouples oligomer dissociation from unfolding
    Hammad Naveed
    Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA
    J Mol Biol 419:89-101. 2012
    ..The bacterial porin family is perhaps the best-studied protein family among β-barrel membrane proteins that allows diffusion of small ..
  98. doi Increased expression of VDAC1 sensitizes carcinoma cells to apoptosis induced by DNA cross-linking agents
    Ossama Sharaf el Dein
    INSERM UMR S 769, LabEx LERMIT, Universite Paris Sud 11, 5 rue J B Clement, Chatenay Malabry, France
    Biochem Pharmacol 83:1172-82. 2012
    ..We further showed that, among the pro-apoptotic PTP regulators tested (VDAC1, creatine kinase, ANT1 and ANT3), exogenous overexpression of VDAC1 was the most effective in enhancing Cisp- and ..
  99. ncbi Alteration in expression of Serratia marcescens porins associated with decreased outer membrane permeability
    T Hashizume
    Tsukuba Research Institute, Banyu Pharmaceutical Co, Ltd, Tsukuba, Japan
    J Antimicrob Chemother 31:21-8. 1993
    The porin expression of two clinical isolates of Serratia marcescens, which overproduced cephalosporinase and had decreased outer membrane permeability, were studied in comparison with those of reference strains...
  100. doi The single mitochondrial porin of Trypanosoma brucei is the main metabolite transporter in the outer mitochondrial membrane
    Mascha Pusnik
    Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
    Mol Biol Evol 26:671-80. 2009
    ..The single T. brucei mitochondrial porin is essential only under growth conditions that depend on oxidative phosphorylation...
  101. ncbi Isolation and characterization of the Omp-PA porin from Porphyromonas asaccharolytica, determination of the omp-PA gene sequence and prediction of Omp-PA protein structure
    Lana Magalashvili
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Anaerobe 13:74-82. 2007
    A single monomeric porin, Omp-PA (37kDa), was isolated from the outer membrane of the gram-negative anaerobic rod Porphyromonas asaccharolytica...

Research Grants87

  1. Study of Mechanisms Leading to Multidrug-Resistant K. pneumoniae and A. baumannii
    John Quale; Fiscal Year: 2007
    ..pneumoniae and A. baumannii: 1) beta-lactamases, 2) porin deficiencies, and 3) antibiotic efflux systems...
  2. Neisserial Porins and Antigen Presenting Cells
    LEE MARK WETZLER; Fiscal Year: 2010
    Our laboratory has been investigating the immunobiology of the Neisserial porin PorB for the last two decades...
  3. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
    ..We recently discovered two porins of M. tuberculosis that complement the permeability defects of a porin mutant of M. smegmatis. Both purified recombinant proteins showed channel activity in lipid bilayer experiments...
  4. REGULATION OF THE HEAT SHOCK RESPONSE IN E. COLI
    CAROL ANNE GROSS; Fiscal Year: 2010
    ..remaining ?E sRNAs and test the hypothesis that they function to provide overall surveillance of outer membrane porin content and critical control of the properties of the stress response...
  5. REGULATION OF THE HEAT SHOCK RESPONSE IN E. COLI
    Carol Gross; Fiscal Year: 2009
    ..remaining ?E sRNAs and test the hypothesis that they function to provide overall surveillance of outer membrane porin content and critical control of the properties of the stress response...
  6. MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINS
    ANNE DELCOUR; Fiscal Year: 2003
    ..Changes in porin expression patterns have also been observed in virulent strains of E. coli causing urinary tract infections...
  7. HOST CELL RECOGNITION OF SALMONELLA TYPHIMURIUM
    THOMAS PISTOLE; Fiscal Year: 2003
    ..b>Porin-deficient mutants will be compared with their corresponding wildtype counterparts in their ability to adhere to ..
  8. Neisserial Porins and Antigen Presenting Cells
    Lee Wetzler; Fiscal Year: 2005
    ..following aims of the proposal will attempt to prove this hypothesis: 1) investigate the interaction of Neisserial porin with TLR2 and demonstrate the importance of TLR2 mediated signaling in their adjuvant activity, 2) explore the ..
  9. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2007
    ..retards the entry of antimicrobial agents, both hydrophilic and lipophilic, because the narrowness or paucity of porin channels makes the entry of hydrophilic agents difficult, and because the lipopolysaccharide-phospholipid ..
  10. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2009
    ..retards the entry of antimicrobial agents, both hydrophilic and lipophilic, because the narrowness or paucity of porin channels makes the entry of hydrophilic agents difficult, and because the lipopolysaccharide-phospholipid ..
  11. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2010
    ..retards the entry of antimicrobial agents, both hydrophilic and lipophilic, because the narrowness or paucity of porin channels makes the entry of hydrophilic agents difficult, and because the lipopolysaccharide-phospholipid ..
  12. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 1993
    ..work from our lab has identified two genes, ompR and envZ, which specify proteins that are required to activate porin gene expression...
  13. LIGAND GATED TRANSPORT THROUGH FEPA
    PHILLIP KLEBBA; Fiscal Year: 2004
    ..FepA is a ligand-gated porin, in that binding of ferric enterobactin triggers transport through its transmembrane pore...
  14. STRUCTURE-FUNCTION STUDIES OF MOLECULAR CHAPERONES
    David McKay; Fiscal Year: 2007
    ..vitro peptide binding activity with in vivo interactions with membrane proteins, and ultimately to develop an in vitro assay of bacterial porin folding and assembly that will allow direct biochemical assay of SurA and related proteins.
  15. Effect of Neisserial Porin in Immune Cell Apoptosis
    Lee Wetzler; Fiscal Year: 2006
    ..We found that the anti-apoptotic effect is due to a direct interaction of the porin with mitochondria (a central control point for apoptotic cell death)...
  16. Genetic screens to identify mycobacterial porins
    Martin Pavelka; Fiscal Year: 2007
    ..Several mycobacterial porins have been physically characterized from whole cell extracts, the porin genes of M. smegmatis have been identified and the major porin gene, MspA, structurally characterized...
  17. PATHOGENIC MECHANISMS IN LYME BORRELIOSIS
    Michael Lovett; Fiscal Year: 2000
    ..Specific Aim 1 involves the characterization of Oms1 and Oms2, outer membrane spanning proteins with porin activity identified during the previous grant period...
  18. Molecular Dissection of the Permeability Transition Pore
    Michael Forte; Fiscal Year: 2007
    ..assays as a high affinity (nM) blocker of the PTP through covalent modification of isoform 1 of mammalian VDAC (VDAC1). Similar experiments have also demonstrated that yeast VDAC1 is specifically targeted by this compound...
  19. REGULATION OF PULMONARY CIRCULATION IN FETUS AND NEWBORN
    Girija G Konduri; Fiscal Year: 2010
    ..and postnatal oxygen tension stimulate the association of eNOS with the mitochondrial outer membrane protein, porin in normal fetal lamb pulmonary artery endothelial cells (PAEC)...
  20. IMMUNOLOGY OF INFECTION WITH NEISSERIA GONORRHOEAE
    Peter Rice; Fiscal Year: 2003
    ..complement pathway regulatory protein factor H to sialylated lipooligosaccharide (LOS) and to loop 5 of porin protein (Por) IA...
  21. High Throughput Protein Production by Novel E. coli Expression-Secretion System
    WILLIAM MACCONNELL; Fiscal Year: 2006
    ..Published work has shown that fusion of coding regions such as: scFv antibody chain, E. coli outer membrane porin (OmpF), chloramphenicol acetyltransferase (CAT), or L-asparagenase genes to the c-terminal 200 amino acid portions ..
  22. PATHOGENESIS OF LIVER CELL NECROSIS
    John Farber; Fiscal Year: 2005
    ..and apoptosis direct physical connections through the cytoskeleton between the outer mitochondrial membrane (porin in particular) and the plasma membrane mediate the energy-independent coupling of the MPT to the loss of viability ..
  23. Penicillin-resistant Neisseria gonorrhoeae (CMRNG)
    Robert Nicholas; Fiscal Year: 2005
    ..In addition, we propose experiments that follow up on our structure/function studies of the penB gene product, porin IB, to understand how mutations in this protein increase both penicillin and tetracycline resistance...