Gene Symbol: VAMP4
Description: vesicle associated membrane protein 4
Alias: VAMP-4, VAMP24, vesicle-associated membrane protein 4, VAMP4/TAF3 fusion
Species: human
Products:     VAMP4

Top Publications

  1. Scales S, Chen Y, Yoo B, Patel S, Doung Y, Scheller R. SNAREs contribute to the specificity of membrane fusion. Neuron. 2000;26:457-64 pubmed
    ..The data suggest that SNARE pairing does underlie vesicle trafficking fidelity, and that specific SNARE interactions with other proteins may facilitate the correct pairing. ..
  2. Peden A, Park G, Scheller R. The Di-leucine motif of vesicle-associated membrane protein 4 is required for its localization and AP-1 binding. J Biol Chem. 2001;276:49183-7 pubmed
    ..We investigate the role of the di-leucine motif of vesicle-associated membrane protein 4 (VAMP4) in adaptor binding and localization of VAMP4...
  3. Mallard F, Tang B, Galli T, Tenza D, Saint Pol A, Yue X, et al. Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. J Cell Biol. 2002;156:653-64 pubmed
    ..t-SNAREs syntaxin 6, syntaxin 16, and Vti1a, and two early/recycling endosomal v-SNAREs, VAMP3/cellubrevin, and VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in the post-Golgi retrograde ..
  4. Gordon D, Mirza M, Sahlender D, Jakovleska J, Peden A. Coiled-coil interactions are required for post-Golgi R-SNARE trafficking. EMBO Rep. 2009;10:851-6 pubmed publisher
    ..VAMPs 3 and 8 are determined solely through a new conserved mechanism that uses coiled-coil interactions, and that VAMP4 does not require these interactions for its trafficking...
  5. Zeng Q, Tran T, Tan H, Hong W. The cytoplasmic domain of Vamp4 and Vamp5 is responsible for their correct subcellular targeting: the N-terminal extenSion of VAMP4 contains a dominant autonomous targeting signal for the trans-Golgi network. J Biol Chem. 2003;278:23046-54 pubmed
    ..The Vamp subfamily of SNAREs contains 7 members (Vamp1, Vamp2, Vamp3/cellubrevin, Vamp4, Vamp5, Vamp7/Ti-Vamp, and Vamp8/endobrevin) that are distributed in various post-Golgi structures...
  6. Advani R, Bae H, Bock J, Chao D, Doung Y, Prekeris R, et al. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J Biol Chem. 1998;273:10317-24 pubmed
    ..Our studies support the hypothesis that evolutionary radiation of these two gene families gave rise to sets of proteins whose differential expression and combinatorial associations define and organize the membrane compartments of cells. ..
  7. Steegmaier M, Klumperman J, Foletti D, Yoo J, Scheller R. Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking. Mol Biol Cell. 1999;10:1957-72 pubmed
    ..b>VAMP4, a recently discovered member of the vesicle-associated membrane protein (VAMP) family of trafficking proteins, ..
  8. Hou Z, Fu Q, Huang Y, Zhang P, Chen F, Li M, et al. Comparative proteomic identification of capacitation and noncapacitation swamp buffalo spermatozoa. Theriogenology. 2019;128:176-183 pubmed publisher
    ..Western blot (WB) assay confirmed the expressional variation of VAMP4 and APOC3 proteins...
  9. Hou Z, Fu Q, Huang Y, Zhang P, Chen F, Li M, et al. Comparative proteomic identification buffalo spermatozoa during in vitro capacitation. Theriogenology. 2019;126:303-309 pubmed publisher
    ..Western blot (WB) assay confirmed the expressional variation of VAMP4 and APOC3 proteins...

More Information


  1. Colombo F, Racchetti G, Meldolesi J. Neurite outgrowth induced by NGF or L1CAM via activation of the TrkA receptor is sustained also by the exocytosis of enlargeosomes. Proc Natl Acad Sci U S A. 2014;111:16943-8 pubmed publisher
    ..and to a lower extent with basic FGF and EGF) induces the appearance of enlargeosome vesicles competent for VAMP4-dependent exocytosis abundant in high REST-PC12 clones...
  2. Jain S, Farías G, Bonifacino J. Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1. Mol Biol Cell. 2015;26:218-28 pubmed publisher
    ..Here we report that polarized sorting of the Cu(2+) transporter ATP7B and the vesicle-SNARE VAMP4 to the somatodendritic domain of rat hippocampal neurons is mediated by recognition of dileucine-based signals in ..
  3. Abascal Palacios G, Schindler C, Rojas A, Bonifacino J, Hierro A. Structural basis for the interaction of the Golgi-Associated Retrograde Protein Complex with the t-SNARE Syntaxin 6. Structure. 2013;21:1698-706 pubmed publisher
    ..vesicles to the trans-Golgi network through interaction with components of the Syntaxin 6/Syntaxin 16/Vti1a/VAMP4 SNARE complex...
  4. Shi X, Halder P, Yavuz H, Jahn R, Shuman H. Direct targeting of membrane fusion by SNARE mimicry: Convergent evolution of Legionella effectors. Proc Natl Acad Sci U S A. 2016;113:8807-12 pubmed publisher
    ..proteins selectively form complexes with the endosomal arginine (R)-SNARE vesicle-associated membrane protein 4 (VAMP4)...
  5. Harriff M, Karamooz E, Burr A, Grant W, Canfield E, Sorensen M, et al. Endosomal MR1 Trafficking Plays a Key Role in Presentation of Mycobacterium tuberculosis Ligands to MAIT Cells. PLoS Pathog. 2016;12:e1005524 pubmed publisher
    ..We identified Stx18, VAMP4, and Rab6 as trafficking molecules regulating MR1-dependent MAIT cell recognition of Mtb-infected cells...
  6. Hirata T, Fujita M, Nakamura S, Gotoh K, Motooka D, Murakami Y, et al. Post-Golgi anterograde transport requires GARP-dependent endosome-to-TGN retrograde transport. Mol Biol Cell. 2015;26:3071-84 pubmed publisher
    ..Overexpression of VAMP4, v-SNARE, in VPS54-KO cells partially restored not only endosome-to-TGN retrograde transport, but also anterograde ..
  7. Oksdath M, Guil A, Grassi D, Sosa L, Quiroga S. The Motor KIF5C Links the Requirements of Stable Microtubules and IGF-1 Receptor Membrane Insertion for Neuronal Polarization. Mol Neurobiol. 2017;54:6085-6096 pubmed publisher
    ..Syntaxin 6 and VAMP4, necessary for the polarized insertion of the IGF-1r, are associated to vesicles carried by the microtubular motor ..
  8. Nicholson Fish J, Kokotos A, Gillingwater T, Smillie K, Cousin M. VAMP4 Is an Essential Cargo Molecule for Activity-Dependent Bulk Endocytosis. Neuron. 2015;88:973-984 pubmed publisher
    ..The retrieval of only one reporter, VAMP4-pHluorin, was perturbed by inhibiting ADBE...
  9. Masuyer G, Zhang S, Barkho S, Shen Y, Henriksson L, Košenina S, et al. Structural characterisation of the catalytic domain of botulinum neurotoxin X - high activity and unique substrate specificity. Sci Rep. 2018;8:4518 pubmed publisher
    ..vesicle associated membrane proteins (VAMP) 1/2/3 at a unique site, but also has the unique ability to cleave VAMP4/5 and Ykt6. Here we report the 1.35 Å X-ray crystal structure of the light chain of BoNT/X (LC/X)...
  10. Monies D, Abouelhoda M, AlSayed M, Alhassnan Z, Alotaibi M, Kayyali H, et al. The landscape of genetic diseases in Saudi Arabia based on the first 1000 diagnostic panels and exomes. Hum Genet. 2017;136:921-939 pubmed publisher
    ..RIMS2, RNF213, ROBO1, SEC16A, SIAH1, SIRT2, SLAIN2, SLC22A20, SMDT1, SRRT, SSTR1, ST20, SYT9, TSPAN6, UBR4, VAMP4, VPS36, WDR59, WDYHV1, and WHSC1) not previously linked to human phenotypes and these are presented to accelerate ..
  11. Liu Q, Wang X, Liu Y, Wei M, Chen L. A combinative analysis of gene expression profiles and microRNA expression profiles identifies critical genes and microRNAs in oral lichen planus. Arch Oral Biol. 2016;68:61-5 pubmed publisher
    ..targets of miR-362 were SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), vesicle-associated membrane protein 4 (VAMP4), leucine rich repeat transmembrane neuronal 4 (LRRTM4) and lysine (K)-specific demethylase5C (KDM5C)...
  12. Swift D, Dunning L, Igea J, Brooks E, Jones C, Noble L, et al. Evidence of positive selection associated with placental loss in tiger sharks. BMC Evol Biol. 2016;16:126 pubmed publisher
    ..these genes have functions likely associated with brain development (YWHAE and ARL6IP5) and sexual reproduction (VAMP4 and TCTEX1D2)...
  13. Sahlender D, Kozik P, Miller S, Peden A, Robinson M. Uncoupling the functions of CALM in VAMP sorting and clathrin-coated pit formation. PLoS ONE. 2013;8:e64514 pubmed publisher
    ..Thus, we can uncouple the sorting function of CALM from its structural role. ..
  14. Raingo J, Khvotchev M, Liu P, Darios F, Li Y, Ramirez D, et al. VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission. Nat Neurosci. 2012;15:738-45 pubmed publisher
    ..drives rapid Ca(2+)-dependent synchronous neurotransmission, whereas the structurally homologous SNARE protein VAMP4 selectively maintains bulk Ca(2+)-dependent asynchronous release...
  15. Krzewski K, Gil Krzewska A, Watts J, Stern J, Strominger J. VAMP4- and VAMP7-expressing vesicles are both required for cytotoxic granule exocytosis in NK cells. Eur J Immunol. 2011;41:3323-9 pubmed publisher
    ..Here, we show that NK cells express all members of the R-SNARE subgroup. Two of these R-SNARE proteins, VAMP4 and VAMP7, colocalize with lytic granules during cytotoxic interactions...
  16. Nicholson Fish J, Smillie K, Cousin M. Monitoring activity-dependent bulk endocytosis with the genetically-encoded reporter VAMP4-pHluorin. J Neurosci Methods. 2016;266:1-10 pubmed publisher
    ..We present here a method to monitor ADBE using the genetically-encoded reporter VAMP4-pHluorin in primary neuronal cultures...
  17. Pérez Victoria F, Schindler C, Magadán J, Mardones G, Delevoye C, Romao M, et al. Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol Biol Cell. 2010;21:3386-95 pubmed publisher
    ..These findings indicate that Ang2 is the missing component of the GARP complex in most eukaryotes. ..
  18. Gomi H, Osawa H, Uno R, Yasui T, Hosaka M, Torii S, et al. Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties. J Histochem Cytochem. 2017;65:637-653 pubmed publisher
    ..and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins VAMP4, VAMP8, syntaxin-2, syntaxin-3, syntaxin-4, and syntaxin-6 were expressed at various levels in individual glands...
  19. Lesteberg K, Orange J, Makedonas G. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res. 2017;65:1031-1045 pubmed publisher
    ..identified by rab8, rab11a, rab4, and rab37 co-localized with new perforin, as well as the SNAREs vti1b and VAMP4. After ablating the function of the recycling endosome pathway, we observed a relative accumulation of new ..
  20. Hinners I, Wendler F, Fei H, Thomas L, Thomas G, Tooze S. AP-1 recruitment to VAMP4 is modulated by phosphorylation-dependent binding of PACS-1. EMBO Rep. 2003;4:1182-9 pubmed
    The R-SNARE VAMP4, which contains a dileucine motif, binds to the AP-1 (adaptor protein-1) subunit mu 1a, but not mu 1b, or the GGAs (Golgi-associated gamma ear containing ARF binding proteins)...
  21. Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan J, et al. The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs). J Biol Chem. 2015;290:28056-69 pubmed publisher
    ..SNAP-47 preferentially interacted with the trans-Golgi network VAMP4 and post-Golgi VAMP7 and -8...
  22. Shitara A, Shibui T, Okayama M, Arakawa T, Mizoguchi I, Sakakura Y, et al. VAMP4 is required to maintain the ribbon structure of the Golgi apparatus. Mol Cell Biochem. 2013;380:11-21 pubmed publisher
    ..Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde ..
  23. Kabeiseman E, Cichos K, Hackstadt T, Lucas A, Moore E. Vesicle-associated membrane protein 4 and syntaxin 6 interactions at the chlamydial inclusion. Infect Immun. 2013;81:3326-37 pubmed publisher
    ..and function of another trans-Golgi SNARE and syntaxin 6-binding partner, vesicle-associated membrane protein 4 (VAMP4), at the chlamydial inclusion...
  24. Hasan N, Corbin D, Hu C. Fusogenic pairings of vesicle-associated membrane proteins (VAMPs) and plasma membrane t-SNAREs--VAMP5 as the exception. PLoS ONE. 2010;5:e14238 pubmed publisher
    ..These data indicate that VAMPs have differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs. ..
  25. Guo B, Liang Q, Li L, Hu Z, Wu F, Zhang P, et al. O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Nat Cell Biol. 2014;16:1215-26 pubmed publisher
    ..O-GlcNAcylated SNAP-29 levels are reduced during starvation in mammalian cells and in C. elegans. Our study reveals a mechanism by which O-GlcNAc-modification integrates nutrient status with autophagosome maturation. ..
  26. Moreno Smith M, Halder J, Meltzer P, Gonda T, Mangala L, Rupaimoole R, et al. ATP11B mediates platinum resistance in ovarian cancer. J Clin Invest. 2013;123:2119-30 pubmed publisher
    ..and with vesicular trafficking proteins, such as syntaxin-6 (STX6) and vesicular-associated membrane protein 4 (VAMP4), strongly suggests that ATP11B contributes to secretory vesicular transport of cisplatin from Golgi to plasma ..
  27. Zhang X, Jiang S, Mitok K, Li L, Attie A, Martin T. BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells. J Cell Biol. 2017;216:2151-2166 pubmed publisher
    ..Ca2+ rises that stimulate DCV exocytosis may stimulate BAIAP3-dependent retrograde trafficking to maintain DCV protein homeostasis and DCV function. ..
  28. Sabra M, Damcott C, Fu M, Ott S, O Connell J, Mitchell B, et al. Vesicle-associated membrane protein 4, a positional candidate gene on 1q24-q25, is not associated with type 2 diabetes in the Old Order Amish. Mol Genet Metab. 2005;85:133-9 pubmed
    The vesicle-associated membrane protein-4 (VAMP4) gene is an excellent type 2 diabetes (T2DM) positional candidate gene. It is located on chromosome 1q24-q25, a region of linkage to T2DM in the Amish and several other populations...