URM1

Summary

Gene Symbol: URM1
Description: ubiquitin related modifier 1
Alias: C9orf74, ubiquitin-related modifier 1, ubiquitin-related modifier 1 homolog
Species: human
Products:     URM1

Top Publications

  1. Qiu R, Wang F, Liu M, Yang Z, Wu T, Ji C. Crystallization and preliminary X-ray analysis of the yeast tRNA-thiouridine modification protein 1 (Tum1p). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:953-5 pubmed publisher
    Yeast tRNA-thiouridine modification protein 1 (Tum1p), a crucial component of the Urm1 system, is believed to play important roles in protein urmylation and tRNA-thiouridine modification...
  2. Lian F, Yu J, Ma X, Yu X, Chen Y, Zhou C. Structural snapshots of yeast alkyl hydroperoxide reductase Ahp1 peroxiredoxin reveal a novel two-cysteine mechanism of electron transfer to eliminate reactive oxygen species. J Biol Chem. 2012;287:17077-87 pubmed publisher
    ..In addition, site-directed mutagenesis in combination with enzymatic assays suggested that the peroxidase activity of Ahp1 would be altered upon the urmylation (covalently conjugated to ubiquitin-related modifier Urm1) of Lys-32.
  3. Tyagi K, Pedrioli P. Protein degradation and dynamic tRNA thiolation fine-tune translation at elevated temperatures. Nucleic Acids Res. 2015;43:4701-12 pubmed publisher
    ..The abundance of Ncs2p and Ncs6p, two members of the URM1 pathway responsible for the thiolation of wobble uridines in cytoplasmic tRNAs tK(UUU), tQ(UUG) and tE(UUC), is ..
  4. Klassen R, Grunewald P, Thüring K, Eichler C, Helm M, Schaffrath R. Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0119261 pubmed publisher
    ..which disable mcm5 side chain formation, or removal of components of the thiolation pathway (Ncs2/Ncs6, Urm1, Uba4) are individually tolerated, the combination of both modification defects has been reported to have lethal ..
  5. Alings F, Sarin L, Fufezan C, Drexler H, Leidel S. An evolutionary approach uncovers a diverse response of tRNA 2-thiolation to elevated temperatures in yeast. RNA. 2015;21:202-12 pubmed publisher
    ..follows accumulation of newly synthesized tRNA that lack s(2)U34 modification due to temperature sensitivity of the URM1 pathway in S. cerevisiae and S. bayanus...
  6. Damon J, Pincus D, Ploegh H. tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:270-82 pubmed publisher
    ..The URM1 pathway is required for thiolation of the cytoplasmic tRNAs tGlu(UUC), tGln(UUG), and tLys(UUU) in Saccharomyces ..
  7. Chowdhury M, Dosche C, Löhmannsröben H, Leimkuhler S. Dual role of the molybdenum cofactor biosynthesis protein MOCS3 in tRNA thiolation and molybdenum cofactor biosynthesis in humans. J Biol Chem. 2012;287:17297-307 pubmed publisher
    ..We showed that MOCS3 activates both MOCS2A and URM1 by adenylation and a subsequent sulfur transfer step for the formation of the thiocarboxylate group at the C ..
  8. Hleihel R, Khoshnood B, Dacklin I, Omran H, Mouawad C, Dassouki Z, et al. The HTLV-1 oncoprotein Tax is modified by the ubiquitin related modifier 1 (Urm1). Retrovirology. 2018;15:33 pubmed publisher
    ..modification landscape of Tax, we here demonstrate that Tax also interacts with the ubiquitin-related modifier 1 (Urm1)...
  9. Khoshnood B, Dacklin I, Grabbe C. Urm1: an essential regulator of JNK signaling and oxidative stress in Drosophila melanogaster. Cell Mol Life Sci. 2016;73:1939-54 pubmed publisher
    Ubiquitin-related modifier 1 (Urm1) is a ubiquitin-like molecule (UBL) with the dual capacity to act both as a sulphur carrier and posttranslational protein modifier...

More Information

Publications23

  1. Klassen R, Ciftci A, Funk J, Bruch A, Butter F, Schaffrath R. tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast. Nucleic Acids Res. 2016;44:10946-10959 pubmed
    ..in anticodon-codon interaction: 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U34: ELP3, URM1), pseudouridine (Ψ38/39: DEG1) and cyclic N6-threonyl-carbamoyl-adenosine (ct6A37: TCD1)...
  2. Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher
    ..i>sap190?, ppm1?, rrd1?) and U34 modification defects (elp3?, kti12?, urm1?, ncs2?) and found the rapamycin hypersensitivity in the latter is epistatic to drug resistance of the ..
  3. Kumar H, Frischknecht F, Mair G, Gomes J. In silico identification of genetically attenuated vaccine candidate genes for Plasmodium liver stage. Infect Genet Evol. 2015;36:72-81 pubmed publisher
    ..twenty GAP-candidates; these are: fabB/F, fabI, arp, 3 genes encoding subunits of the PDH complex, dnaJ, urm1, rS5, ancp, mcp, arh, gk, lisp2, valS, palm, and four conserved Plasmodium proteins of unknown function...
  4. Cappadocia L, Lima C. Ubiquitin-like Protein Conjugation: Structures, Chemistry, and Mechanism. Chem Rev. 2018;118:889-918 pubmed publisher
    ..Precisely, we will focus on conjugation machinery in the SUMO, NEDD8, ATG8, ATG12, URM1, UFM1, FAT10, and ISG15 pathways while referring to the ubiquitin pathway to highlight common or contrasting themes...
  5. van der Veen A, Schorpp K, Schlieker C, Buti L, Damon J, Spooner E, et al. Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier. Proc Natl Acad Sci U S A. 2011;108:1763-70 pubmed publisher
    The ubiquitin (Ub)-related modifier Urm1 functions as a sulfur carrier in tRNA thiolation by means of a mechanism that requires the formation of a thiocarboxylate at the C-terminal glycine residue of Urm1...
  6. Schlieker C, van der Veen A, Damon J, Spooner E, Ploegh H. A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway. Proc Natl Acad Sci U S A. 2008;105:18255-60 pubmed publisher
    b>Urm1 is a highly conserved ubiquitin-related modifier of unknown function. A reduction of cellular Urm1 levels causes severe cytokinesis defects in HeLa cells, resulting in the accumulation of enlarged multinucleated cells...
  7. Jüdes A, Ebert F, Bär C, Thüring K, Harrer A, Klassen R, et al. Urmylation and tRNA thiolation functions of ubiquitin-like Uba4·Urm1 systems are conserved from yeast to man. FEBS Lett. 2015;589:904-9 pubmed publisher
    The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways...
  8. Klassen R, Bruch A, Schaffrath R. Independent suppression of ribosomal +1 frameshifts by different tRNA anticodon loop modifications. RNA Biol. 2017;14:1252-1259 pubmed publisher
    ..a heat-induced increase of +1 frameshift rates in wild type cells that depends on the sulfur transfer protein Urm1. Furthermore, we find that temperature-induced frameshifting is kept in check by the dehydration of N6-..
  9. Wang Z, Zhu W, Xu X. Ubiquitin-like modifications in the DNA damage response. Mutat Res. 2017;803-805:56-75 pubmed publisher
    ..gene 15 (ISG15), human leukocyte antigen (HLA)-F adjacent transcript 10 (FAT10), ubiquitin-fold modifier 1 (UFRM1), URM1 ubiquitin-related modifier-1 (URM1), autophagy-related protein 12 (ATG12), autophagy-related protein 8 (ATG8), fan ..
  10. Petroski M, Salvesen G, Wolf D. Urm1 couples sulfur transfer to ubiquitin-like protein function in oxidative stress. Proc Natl Acad Sci U S A. 2011;108:1749-50 pubmed publisher
  11. Kim S, Johnson W, Chen C, Sewell A, Byström A, Han M. Allele-specific suppressors of lin-1(R175Opal) identify functions of MOC-3 and DPH-3 in tRNA modification complexes in Caenorhabditis elegans. Genetics. 2010;185:1235-47 pubmed publisher
    ..We further showed that loss-of-function mutations of urm-1 and elpc-1-4, the worm counterparts of URM1 and ELP complex components in yeast, also suppressed lin-1(e1275)...
  12. Xu J, Zhang J, Wang L, Zhou J, Huang H, Wu J, et al. Solution structure of Urm1 and its implications for the origin of protein modifiers. Proc Natl Acad Sci U S A. 2006;103:11625-30 pubmed
    ..between the protein modifiers and sulfur carrier proteins, we solved the solution NMR structure of the Urm1 (ubiquitin-related modifier-1) protein from Saccharomyces cerevisiae...
  13. Singh S, Tonelli M, Tyler R, Bahrami A, Lee M, Markley J. Three-dimensional structure of the AAH26994.1 protein from Mus musculus, a putative eukaryotic Urm1. Protein Sci. 2005;14:2095-102 pubmed
    ..musculus and propose that it represents the first three-dimensional structure of a ubiquitin-related modifier 1 (Urm1) protein. Amino acid sequence comparisons indicate that AAH26994...
  14. Schmitz J, Chowdhury M, Hänzelmann P, Nimtz M, Lee E, Schindelin H, et al. The sulfurtransferase activity of Uba4 presents a link between ubiquitin-like protein conjugation and activation of sulfur carrier proteins. Biochemistry. 2008;47:6479-89 pubmed publisher
    ..of ubiquitin and the biosynthesis of several sulfur-containing cofactors, we have characterized the human Urm1 and Saccharomyces cerevisiae Uba4 proteins, which are very similar in sequence to MOCS2A and MOCS3, respectively, ..