Gene Symbol: UL25
Description: located on capsid near vertices; possibly stabilizes the capsid and retains the genome; involved in DNA encapsidation
Alias: located on capsid near vertices, possibly stabilizes the capsid and retains the genome, involved in DNA encapsidation, DNA packaging tegument protein UL25
Species: Human herpesvirus 1

Top Publications

  1. Trus B, Newcomb W, Cheng N, Cardone G, Marekov L, Homa F, et al. Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids. Mol Cell. 2007;26:479-89 pubmed
    b>UL25 and UL17 are two essential minor capsid proteins of HSV-1, implicated in DNA packaging and capsid maturation...
  2. Conway J, Cockrell S, Copeland A, Newcomb W, Brown J, Homa F. Labeling and localization of the herpes simplex virus capsid protein UL25 and its interaction with the two triplexes closest to the penton. J Mol Biol. 2010;397:575-86 pubmed publisher
    The herpes simplex virus type 1 UL25 protein is one of seven viral proteins that are required for DNA cleavage and packaging...
  3. Pasdeloup D, Blondel D, Isidro A, Rixon F. Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25. J Virol. 2009;83:6610-23 pubmed publisher
  4. Bowman B, Welschhans R, Jayaram H, Stow N, Preston V, Quiocho F. Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1. J Virol. 2006;80:2309-17 pubmed
    ..The herpes simplex virus type 1 UL25 protein is incorporated into the capsid during DNA packaging, and previous studies of a null mutant have ..
  5. Schipke J, Pohlmann A, Diestel R, Binz A, Rudolph K, Nagel C, et al. The C terminus of the large tegument protein pUL36 contains multiple capsid binding sites that function differently during assembly and cell entry of herpes simplex virus. J Virol. 2012;86:3682-700 pubmed publisher
    ..Capsids lacking pUL36 are targeted neither to membranes for virus assembly nor to nuclear pores for genome uncoating. ..
  6. Cockrell S, Huffman J, Toropova K, Conway J, Homa F. Residues of the UL25 protein of herpes simplex virus that are required for its stable interaction with capsids. J Virol. 2011;85:4875-87 pubmed publisher
    The herpes simplex virus 1 (HSV-1) UL25 gene product is a minor capsid component that is required for encapsidation, but not cleavage, of replicated viral DNA...
  7. Preston V, Murray J, Preston C, McDougall I, Stow N. The UL25 gene product of herpes simplex virus type 1 is involved in uncoating of the viral genome. J Virol. 2008;82:6654-66 pubmed publisher
    Studies on the herpes simplex virus type 1 UL25-null mutant KUL25NS have shown that the capsid-associated UL25 protein is required at a late stage in the encapsidation of viral DNA...
  8. Cockrell S, Sanchez M, Erazo A, Homa F. Role of the UL25 protein in herpes simplex virus DNA encapsidation. J Virol. 2009;83:47-57 pubmed publisher
    The herpes simplex virus protein UL25 attaches to the external vertices of herpes simplex virus type 1 capsids and is required for the stable packaging of viral DNA...
  9. O Hara M, Rixon F, Stow N, Murray J, Murphy M, Preston V. Mutational analysis of the herpes simplex virus type 1 UL25 DNA packaging protein reveals regions that are important after the viral DNA has been packaged. J Virol. 2010;84:4252-63 pubmed publisher
    The herpes simplex virus type 1 (HSV-1) UL25 gene encodes a minor capsid protein, pUL25, that is essential for packaging the full-length viral genome...

More Information


  1. Thurlow J, Murphy M, Stow N, Preston V. Herpes simplex virus type 1 DNA-packaging protein UL17 is required for efficient binding of UL25 to capsids. J Virol. 2006;80:2118-26 pubmed
    ..Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently...
  2. Scholtes L, Baines J. Effects of major capsid proteins, capsid assembly, and DNA cleavage/packaging on the pUL17/pUL25 complex of herpes simplex virus 1. J Virol. 2009;83:12725-37 pubmed publisher
    ..The data indicate that the pU(L)17/pU(L)25 complex requires multiple viral proteins and functions for proper localization and biochemical behavior in the infected cell. ..
  3. Snijder J, Radtke K, Anderson F, Scholtes L, Corradini E, Baines J, et al. Vertex-Specific Proteins pUL17 and pUL25 Mechanically Reinforce Herpes Simplex Virus Capsids. J Virol. 2017;91: pubmed publisher
    ..These new insights support us in forming a complete view on viral structure and furthermore could possibly help not only to develop specific antivirals but also to build protein shells with improved stability for drug delivery purposes. ..
  4. Zhang Y, Li Y, Liu L, Jiang L, Ji M, Jiang R, et al. Host cell protein C9orf69 promotes viral proliferation via interaction with HSV-1 UL25 protein. Virol Sin. 2011;26:171-80 pubmed publisher
    In light of the scarcity of reports on the interaction between HSV-1 nucleocapsid protein UL25 and its host cell proteins, the purpose of this study is to use yeast two-hybrid screening to search for cellular proteins that can interact ..
  5. Yang K, Baines J. Selection of HSV capsids for envelopment involves interaction between capsid surface components pUL31, pUL17, and pUL25. Proc Natl Acad Sci U S A. 2011;108:14276-81 pubmed publisher
    ..The interaction between the CCSC and pU(L)31 in the NEC suggests a mechanism to conserve viral resources by promoting assembly of only those viral particles with the potential to become infectious. ..
  6. Sae Ueng U, Liu T, Catalano C, Huffman J, Homa F, Evilevitch A. Major capsid reinforcement by a minor protein in herpesviruses and phage. Nucleic Acids Res. 2014;42:9096-107 pubmed publisher
    ..Our data reveal that the loosely formed capsid structure is reinforced post-assembly by the minor capsid protein UL25 in HSV-1 and gpD in bacteriophage ?...
  7. Huffman J, Daniel G, Falck Pedersen E, Huet A, Smith G, Conway J, et al. The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores. J Virol. 2017;91: pubmed publisher
    ..pUL25 is found at each of the capsid vertices as part of the capsid vertex-specific component and implicates the importance of this complex for NPC binding and genome release. ..
  8. Yang K, Wills E, Lim H, Zhou Z, Baines J. Association of herpes simplex virus pUL31 with capsid vertices and components of the capsid vertex-specific complex. J Virol. 2014;88:3815-25 pubmed publisher
    ..It also shows this interaction is dependent on the C terminus of U(L)25. This information is vital for understanding how capsids bud through the inner nuclear membrane. ..
  9. Fan W, Roberts A, McElwee M, Bhella D, Rixon F, Lauder R. The large tegument protein pUL36 is essential for formation of the capsid vertex-specific component at the capsid-tegument interface of herpes simplex virus 1. J Virol. 2015;89:1502-11 pubmed publisher
    ..This demonstrates the importance of pUL36 in the initial stages of tegument addition and provides new insights into the process of virus particle assembly. ..