UBE2G2

Summary

Gene Symbol: UBE2G2
Description: ubiquitin conjugating enzyme E2 G2
Alias: UBC7, ubiquitin-conjugating enzyme E2 G2, E2 ubiquitin-conjugating enzyme G2, ubiquitin carrier protein G2, ubiquitin conjugating enzyme 7, ubiquitin conjugating enzyme G2, ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast), ubiquitin-conjugating enzyme E2G 2 (homologous to yeast UBC7), ubiquitin-protein ligase G2
Species: human
Products:     UBE2G2

Top Publications

  1. Cao J, Wang J, Qi W, Miao H, Wang J, Ge L, et al. Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase. Cell Metab. 2007;6:115-28 pubmed
    ..In summary, our study identifies Ufd1 as a cofactor of gp78, reveals an unappreciated function of Ufd1 in the ubiquitination reaction during ERAD, and illustrates that Ufd1 plays a critical role in cholesterol metabolism. ..
  2. Li W, Tu D, Li L, Wollert T, Ghirlando R, Brunger A, et al. Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Proc Natl Acad Sci U S A. 2009;106:3722-7 pubmed publisher
    ..gp78 can mediate the preassembly of Lys-48-linked polyubiquitin chains on the catalytic cysteine of its cognate E2 Ube2g2 and subsequent transfer to a substrate...
  3. Spandl J, Lohmann D, Kuerschner L, Moessinger C, Thiele C. Ancient ubiquitous protein 1 (AUP1) localizes to lipid droplets and binds the E2 ubiquitin conjugase G2 (Ube2g2) via its G2 binding region. J Biol Chem. 2011;286:5599-606 pubmed publisher
    ..We show that AUP1, by means of its G2BR domain, binds to Ube2g2. This binding is abolished by deletion or mutation of the G2BR domain, although the LD localization of AUP1 is not ..
  4. Klemm E, Spooner E, Ploegh H. Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control. J Biol Chem. 2011;286:37602-14 pubmed publisher
    ..One of the functions of AUP1 in ER quality control is to recruit the soluble E2 ubiquitin-conjugating enzyme UBE2G2. We further show that the CUE domain of AUP1 regulates polyubiquitylation and facilitates the interaction of AUP1 ..
  5. Li W, Tu D, Brunger A, Ye Y. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature. 2007;446:333-7 pubmed
    ..Here we dissect this process using mouse Ube2g2 (E2; identical at the amino acid level to human Ube2g2) and human gp78 (E3), an endoplasmic reticulum (ER)-..
  6. Morito D, Hirao K, Oda Y, Hosokawa N, Tokunaga F, Cyr D, et al. Gp78 cooperates with RMA1 in endoplasmic reticulum-associated degradation of CFTRDeltaF508. Mol Biol Cell. 2008;19:1328-36 pubmed publisher
    ..Our data demonstrates that gp78 cooperates with RMA1 with E4-like activity in the ERAD of CFTRDeltaF508. ..
  7. Chen B, Mariano J, Tsai Y, Chan A, Cohen M, Weissman A. The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Proc Natl Acad Sci U S A. 2006;103:341-6 pubmed
    ..In this study, we establish that a human ubiquitin ligase (E3), gp78, and a specific E2, Ube2g2, are both critically important for ERAD of multiple substrates...
  8. Wang Q, Liu Y, Soetandyo N, Baek K, HEGDE R, Ye Y. A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation. Mol Cell. 2011;42:758-70 pubmed publisher
    ..Our results reveal a ubiquitin ligase-associated holdase that maintains polypeptide solubility to enhance protein quality control in mammalian cells. ..
  9. Fang S, Ferrone M, Yang C, Jensen J, Tiwari S, Weissman A. The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2001;98:14422-7 pubmed
    ..gp78 has thus been found to be an example of a mammalian cellular E3 intrinsic to the ER, suggesting a potential link between ubiquitylation, ERAD, and metastasis. ..

More Information

Publications68

  1. Das R, Liang Y, Mariano J, Li J, Huang T, King A, et al. Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine. EMBO J. 2013;32:2504-16 pubmed publisher
    ..gp78 includes a high-affinity secondary binding region for its cognate E2, Ube2g2, the G2BR. The G2BR allosterically enhances RING:Ube2g2 binding and ubiquitination...
  2. Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, et al. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014;33:46-61 pubmed publisher
    ..gp78 ubiquitinates misfolded proteins by transferring preformed Lys48-linked ubiquitin chains from the cognate E2 Ube2g2 to substrates...
  3. Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed publisher
    ..In the absence of both RNF145 and gp78, Hrd1, a third UBE2G2-dependent E3 ligase, partially regulates HMGCR activity...
  4. Wang Y, Guan S, Acharya P, Liu Y, Thirumaran R, Brandman R, et al. Multisite phosphorylation of human liver cytochrome P450 3A4 enhances Its gp78- and CHIP-mediated ubiquitination: a pivotal role of its Ser-478 residue in the gp78-catalyzed reaction. Mol Cell Proteomics. 2012;11:M111.010132 pubmed publisher
    ..CYP3A4 turnover occurs via a classical ER-associated degradation (ERAD) process involving ubiquitination by both UBC7/gp78 and UbcH5a/CHIP E2-E3 complexes for 26 S proteasomal targeting...
  5. Koch B, Jin H, Tomko R, Yu H. The anaphase-promoting complex regulates the degradation of the inner nuclear membrane protein Mps3. J Cell Biol. 2019;218:839-854 pubmed publisher
    ..Turnover of Mps3 requires the ubiquitin-conjugating enzyme Ubc7, but was independent of the known ERAD ubiquitin ligases Doa10 and Hrd1 as well as the recently discovered Asi1-..
  6. Wang Y, Kim S, Trnka M, Liu Y, Burlingame A, Correia M. Human liver cytochrome P450 3A4 ubiquitination: molecular recognition by UBC7-gp78 autocrine motility factor receptor and UbcH5a-CHIP-Hsc70-Hsp40 E2-E3 ubiquitin ligase complexes. J Biol Chem. 2015;290:3308-32 pubmed publisher
    ..Ub)-dependent 26 S proteasomal system that relies on two highly complementary E2 Ub-conjugating-E3 Ub-ligase (UBC7-gp78 and UbcH5a-C terminus of Hsc70-interacting protein (CHIP)-Hsc70-Hsp40) complexes, as well as protein kinases (..
  7. Bordallo J, Plemper R, Finger A, Wolf D. Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins. Mol Biol Cell. 1998;9:209-22 pubmed
    ..Interestingly, in ubc6-ubc7 double mutants, CPY* accumulates in the ER, indicating the necessity of an intact cytoplasmic proteolysis machinery ..
  8. Tan J, Cook E, van den Berg M, Scheij S, Zelcer N, Loregger A. Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis. Atherosclerosis. 2019;281:137-142 pubmed publisher
    ..as the primary E2 ubiquitin conjugating enzyme essential for this process in mammalian cells, in contrast to UBE2G2, which is essential for sterol-stimulated degradation of HMGCR...
  9. Wang C, Shi G, Ji X. Design, synthesis, and anticancer activity evaluation of irreversible allosteric inhibitors of the ubiquitin-conjugating enzyme Ube2g2. Medchemcomm. 2018;9:1818-1825 pubmed publisher
    ..The protein interacts with its cognate ubiquitin-conjugating enzyme (E2), Ube2g2, via its RING domain and a unique region called G2BR that strongly binds to E2...
  10. Cohen I, Wiener R, Reiss Y, Ravid T. Distinct activation of an E2 ubiquitin-conjugating enzyme by its cognate E3 ligases. Proc Natl Acad Sci U S A. 2015;112:E625-32 pubmed publisher
    ..However, both E3s bind to and activate a common E2-conjugating enzyme, Ubc7. Here we describe a novel feature of the ERAD system that entails differential activation of Ubc7 by its cognate ..
  11. Bernardi K, Williams J, Kikkert M, van Voorden S, Wiertz E, Ye Y, et al. The E3 ubiquitin ligases Hrd1 and gp78 bind to and promote cholera toxin retro-translocation. Mol Biol Cell. 2010;21:140-51 pubmed publisher
    ..show that expression of mutant Hrd1 and gp78 and a mutant E2-conjugating enzyme dedicated to retro-translocation (Ube2g2) decrease CTA1 retro-translocation. Hrd1 knockdown also attenuated toxin retro-translocation...
  12. Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus D, Le Dez G, et al. Protein quality control at the inner nuclear membrane. Nature. 2014;516:410-3 pubmed publisher
    ..integral membrane E3 ubiquitin ligases Hrd1 and Doa10 operating with the E2 ubiquitin-conjugating enzymes Ubc6 and Ubc7 (refs 2, 3). However, little is known about protein quality control at the INM...
  13. Das R, Mariano J, Tsai Y, Kalathur R, Kostova Z, Li J, et al. Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78. Mol Cell. 2009;34:674-85 pubmed publisher
    ..The G2BR domain within the E3 gp78 binds selectively and with high affinity to the E2 Ube2g2. Through structural and functional analyses, we determine that this occurs on a region of Ube2g2 distinct from ..
  14. Ma H, Dang Y, Wu Y, Jia G, Anaya E, Zhang J, et al. A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death. Cell Rep. 2015;12:673-83 pubmed publisher
    ..Among the genes identified, seven genes, EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, and HRD1, stood out as having the strongest phenotype, whose knockout conferred strong protection against ..
  15. Kumari R, Gupta P, Tiwari S. Ubc7/Ube2g2 ortholog in Entamoeba histolytica: connection with the plasma membrane and phagocytosis. Parasitol Res. 2018;117:1599-1611 pubmed publisher
    ..We have identified orthologs of ER-associated ubiquitin conjugating enzymes (E2s) Ubc6/Ube2j2 and Ubc7/Ube2g2, ubiquitin ligases (E3) Hrd1 and GP78/AMFR, and sensor of UPR, Ire1 in E...
  16. Hantouche C, Williamson B, Valinsky W, Solomon J, Shrier A, Young J. Bag1 Co-chaperone Promotes TRC8 E3 Ligase-dependent Degradation of Misfolded Human Ether a Go-Go-related Gene (hERG) Potassium Channels. J Biol Chem. 2017;292:2287-2300 pubmed publisher
    ..with Bag1 then shifts hERG degradation to the membrane-anchored E3 ligase TRC8 and its E2-conjugating enzyme Ube2g2, as determined by siRNA screening...
  17. Chakrabarti K, Li J, Das R, Byrd R. Conformational Dynamics and Allostery in E2:E3 Interactions Drive Ubiquitination: gp78 and Ube2g2. Structure. 2017;25:794-805.e5 pubmed publisher
    ..The ubiquitin-conjugating enzyme (E2) Ube2g2 functions with the ubiquitin ligase (E3) gp78 to assemble poly-ubiquitin chains on target substrates...
  18. Barrera M, Aguilera S, Castro I, Cortes J, Bahamondes V, Quest A, et al. Pro-inflammatory cytokines enhance ERAD and ATF6? pathway activity in salivary glands of Sjögren's syndrome patients. J Autoimmun. 2016;75:68-81 pubmed publisher
    ..cleavage fragment, and increased expression of ERAD machinery components, such as EDEM1, p97, SEL1L, gp78, UBE2J1, UBE2G2, HERP and DERLIN1, were observed compared to controls...
  19. Weber A, Cohen I, Popp O, Dittmar G, Reiss Y, Sommer T, et al. Sequential Poly-ubiquitylation by Specialized Conjugating Enzymes Expands the Versatility of a Quality Control Ubiquitin Ligase. Mol Cell. 2016;63:827-39 pubmed publisher
    ..These Ub moieties serve as primers for subsequent poly-ubiquitylation by Ubc7. We propose that the evolutionary conserved propensity of Ubc6 to mount Ub on diverse amino acids augments the ..
  20. Hickey C, Hochstrasser M. STUbL-mediated degradation of the transcription factor MATα2 requires degradation elements that coincide with corepressor binding sites. Mol Biol Cell. 2015;26:3401-12 pubmed publisher
    ..known to be ubiquitylated by two distinct pathways, one involving the ubiquitin-conjugating enzymes (E2s) Ubc6 and Ubc7 and the ubiquitin ligase (E3) Doa10 and the other operating with the E2 Ubc4 and the heterodimeric E3 Slx5/Slx8...
  21. von Delbrück M, Kniss A, Rogov V, Pluska L, Bagola K, Lohr F, et al. The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation. Mol Cell. 2016;62:918-928 pubmed publisher
    ..Only recently has ubiquitin binding activity emerged as an important factor in chain formation. The Ubc7 activator Cue1 carries a ubiquitin binding CUE domain that substantially stimulates K48-linked polyubiquitination ..
  22. de Ruijter J, Frey A. Analysis of antibody production in Saccharomyces cerevisiae: effects of ER protein quality control disruption. Appl Microbiol Biotechnol. 2015;99:9061-71 pubmed publisher
    ..on production of a human IgG, we disrupted ERAD at different stages by deletion of the HTM1, YOS9, HRD1, HRD3, or UBC7 gene, with or without a disruption of the UPR by deletion of the IRE1 gene...
  23. Laqqan M, Tierling S, Alkhaled Y, Loporto C, Hammadeh M. Alterations in sperm DNA methylation patterns of oligospermic males. Reprod Biol. 2017;17:396-400 pubmed publisher
    ..in more than one CpG site: there was a significant alteration in methylation levels at all CpGs tested within the UBE2G2 and cg25750688 site related amplicon (p?0...
  24. Pantazopoulou M, Boban M, Foisner R, Ljungdahl P. Cdc48 and Ubx1 participate in a pathway associated with the inner nuclear membrane that governs Asi1 degradation. J Cell Sci. 2016;129:3770-3780 pubmed
    ..by nucleus-localized proteasomes, exhibiting a clear requirement for the E2 ubiquitin-conjugating enzyme Ubc7, Cue1 and the AAA ATPase Cdc48 and co-factor Ubx1...
  25. Katsanis N, Fisher E. Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae ubc7 gene. Genomics. 1998;51:128-31 pubmed
    ..Several of these have been identified in a variety of organisms. Here we report the identification of UBE2G2, a human homologue of the yeast Ubc7 gene. We describe a 2...
  26. Zhong Y, Shen H, Wang Y, Yang Y, Yang P, Fang S. Identification of ERAD components essential for dislocation of the null Hong Kong variant of α-1-antitrypsin (NHK). Biochem Biophys Res Commun. 2015;458:424-8 pubmed publisher
    ..In addition, knockdown of 7 of the 21 components enhanced NHK dislocation. This study uncovers a complex functional network of proteins required for NHK dislocation. ..
  27. Laqqan M, Hammadeh M. Alterations in DNA methylation patterns and gene expression in spermatozoa of subfertile males. Andrologia. 2018;50: pubmed publisher
    ..cg05813498 and cg23081194) have the highest difference in methylation levels and located within ALS2CR12, GAA and UBE2G2 genes respectively; these were selected for further analysis using deep bisulphite sequencing and qPCR in 136 ..
  28. Reyes L, Sommer C, Beltramini L, Henrique Silva F. Expression, purification, and structural analysis of (HIS)UBE2G2 (human ubiquitin-conjugating enzyme). Protein Expr Purif. 2006;45:324-8 pubmed
    ..In the present study, the open reading frame of the human ubiquitin-conjugating enzyme UBE2G2 was isolated from a human brain cDNA panel, cloned into pET28a vector and expressed in Escherichia coli...
  29. Yan L, Liu W, Zhang H, Liu C, Shang Y, Ye Y, et al. Ube2g2-gp78-mediated HERP polyubiquitylation is involved in ER stress recovery. J Cell Sci. 2014;127:1417-27 pubmed publisher
    ..Here we show that, after ER stress induction, HERP is rapidly degraded by Ube2g2-gp78-mediated ubiquitylation and proteasomal degradation...
  30. Tcherpakov M, Delaunay A, Toth J, Kadoya T, Petroski M, Ronai Z. Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP). J Biol Chem. 2009;284:12099-109 pubmed publisher
    ..Significantly, the RNF5 effect on JAMP is seen prior to and after ER stress response, thereby highlighting a novel mechanism to limit ERAD and proteasome assembly at the ER, to the actual ER stress response. ..
  31. Huang L, Kinnucan E, Wang G, Beaudenon S, Howley P, Huibregtse J, et al. Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade. Science. 1999;286:1321-6 pubmed
    ..The crystal structure of the E6AP hect domain bound to the UbcH7 ubiquitin-conjugating enzyme (E2) reveals the determinants of E2-E3 specificity and provides insights into the transfer of ubiquitin from the E2 to the E3. ..
  32. Lenk U, Sommer T. Ubiquitin-mediated proteolysis of a short-lived regulatory protein depends on its cellular localization. J Biol Chem. 2000;275:39403-10 pubmed
    ..These observations imply that both the cellular targeting of a substrate and the compartment-specific activity of components of the ubiquitin-proteasome system define the half-life of naturally short-lived proteins. ..
  33. Mallampalli R, Glasser J, Coon T, Chen B. Calmodulin protects Aurora B on the midbody to regulate the fidelity of cytokinesis. Cell Cycle. 2013;12:663-73 pubmed publisher
    ..These observations indicate molecular interplay between Aurora B and calmodulin in telophase and suggest that calmodulin acts as a checkpoint sensor for chromosomal segregation errors during mitosis. ..
  34. Jo Y, Hartman I, DeBose Boyd R. Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes. Mol Biol Cell. 2013;24:169-83 pubmed publisher
    ..RNA interference (RNAi) studies show that Aup1 recruits the ubiquitin-conjugating enzyme Ubc7 to lipid droplets and facilitates its binding to both gp78 and Trc8...
  35. Chaugule V, Burchell L, Barber K, Sidhu A, Leslie S, Shaw G, et al. Autoregulation of Parkin activity through its ubiquitin-like domain. EMBO J. 2011;30:2853-67 pubmed publisher
    ..Our observations provide important molecular insights into the underlying basis of Parkinson's disease, and in the regulation of RBR E3-ligase activity. ..
  36. Bocik W, Sircar A, Gray J, Tolman J. Mechanism of polyubiquitin chain recognition by the human ubiquitin conjugating enzyme Ube2g2. J Biol Chem. 2011;286:3981-91 pubmed publisher
    b>Ube2g2 is a human ubiquitin conjugating (E2) enzyme involved in the endoplasmic reticulum-associated degradation pathway, which is responsible for the identification and degradation of unfolded and misfolded proteins in the endoplasmic ..
  37. Gao F, Shang Y, Liu W, Li W. The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination. Biochem Biophys Res Commun. 2016;473:1139-1143 pubmed publisher
    ..gp78 and its cognate E2-Ube2g2 catalyze lysine48 (K48)-linked polyubiquitin chains to promote the degradation of targeted proteins...
  38. van de Weijer M, Schuren A, van den Boomen D, Mulder A, Claas F, Lehner P, et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci. 2017;130:2883-2892 pubmed publisher
    ..We identified multiple E2 enzymes involved in this process, of which UBE2G2 was crucial for the degradation of various immunoreceptors...
  39. Liu W, Shang Y, Li W. gp78 elongates of polyubiquitin chains from the distal end through the cooperation of its G2BR and CUE domains. Sci Rep. 2014;4:7138 pubmed publisher
    ..The ER quality control protein E3 gp78 catalyzes Lys48-linked polyubiquitin-chain- assembly on the Ube2g2 active site and is capable of transferring preassembled ubiquitin chains to its substrates...
  40. Sato T, Sako Y, Sho M, Momohara M, Suico M, Shuto T, et al. STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein. Mol Cell. 2012;47:99-110 pubmed publisher
    ..Hence we postulate that STT3B-dependent posttranslational N-glycosylation is part of a triage-salvage system recognizing cryptic N-glycosylation sites of secretory proteins to preserve protein homeostasis. ..
  41. Joazeiro C, Hunter T. Biochemistry. Ubiquitination--more than two to tango. Science. 2000;289:2061-2 pubmed
    ..In their Perspective, Joazeiro and Hunter discuss new structural findings that reveal the part played by an E3 called c-Cbl in this ubiquitinating process. ..
  42. Rose S, Leek J, Moynihan T, Ardley H, Markham A, Robinson P. Assignment1 of the ubiquitin conjugating enzyme gene, UBE2G2, to human chromosome band 21q22.3 by in situ hybridization. Cytogenet Cell Genet. 1998;83:98-9 pubmed
  43. Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama K, et al. CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 2002;10:55-67 pubmed
    ..Furthermore, CHIP enhanced the ability of Parkin to inhibit cell death induced by Pael-R. Taken together, these results indicate that CHIP is a mammalian E4-like molecule that positively regulates Parkin E3 activity. ..
  44. Shibata E, Abbas T, Huang X, Wohlschlegel J, Dutta A. Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. Mol Cell Biol. 2011;31:3136-45 pubmed publisher
    ..On the other hand, members of the UBE2G family of UBCs (UBE2G1 and UBE2G2) cooperate with CRL4(Cdt2) to polyubiquitylate and degrade Cdt1 postradiation, an activity that is critical for ..
  45. Webster J, Tiwari S, Weissman A, Wojcikiewicz R. Inositol 1,4,5-trisphosphate receptor ubiquitination is mediated by mammalian Ubc7, a component of the endoplasmic reticulum-associated degradation pathway, and is inhibited by chelation of intracellular Zn2+. J Biol Chem. 2003;278:38238-46 pubmed
    ..Finally, muscarinic agonist-induced InsP3R ubiquitination was seen in rat brain slices, indicating that the results obtained from SH-SY5Y cells reflect a physiological process. ..
  46. Zou C, Butler P, Coon T, Smith R, Hammen G, Zhao Y, et al. LPS impairs phospholipid synthesis by triggering beta-transducin repeat-containing protein (beta-TrCP)-mediated polyubiquitination and degradation of the surfactant enzyme acyl-CoA:lysophosphatidylcholine acyltransferase I (LPCAT1). J Biol Chem. 2011;286:2719-27 pubmed publisher
    ..LPCAT1 is the first lipogenic substrate for ?-TrCP, and the results suggest that modulation of the GSK-3?-SCF?(TrCP) E3 ligase effector pathway might be a unique strategy to optimize dipalmitoylphosphatidylcholine levels in sepsis. ..
  47. Durcan T, Kontogiannea M, Thorarinsdottir T, Fallon L, WILLIAMS A, Djarmati A, et al. The Machado-Joseph disease-associated mutant form of ataxin-3 regulates parkin ubiquitination and stability. Hum Mol Genet. 2011;20:141-54 pubmed publisher
  48. Ju T, Bocik W, Majumdar A, Tolman J. Solution structure and dynamics of human ubiquitin conjugating enzyme Ube2g2. Proteins. 2010;78:1291-301 pubmed publisher
    b>Ube2g2 is an E2 enzyme which functions as part of the endoplasmic reticulum-associated degradation (ERAD) pathway responsible for identification and degradation of misfolded proteins in the endoplasmic reticulum...
  49. Chen P, Johnson P, Sommer T, Jentsch S, Hochstrasser M. Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor. Cell. 1993;74:357-69 pubmed
    ..Here we show that four UBC proteins (UBC4, UBC5, UBC6, and UBC7) target the yeast MAT alpha 2 transcriptional regulator for intracellular degradation by two distinct ..
  50. Sadri H, Steinhoff Wagner J, Hammon H, Bruckmaier R, Görs S, Sauerwein H. Mammalian target of rapamycin signaling and ubiquitin proteasome-related gene expression in 3 different skeletal muscles of colostrum- versus formula-fed calves. J Dairy Sci. 2017;100:9428-9441 pubmed publisher
    ..conjugating enzyme 1 (UBE2G1) enzymes was not affected by diet, whereas that of ubiquitin conjugating enzyme 2 (UBE2G2) was greater (MLD) or tended to be greater (MM) in COL than in FOR...
  51. Ambivero C, Cilenti L, Main S, Zervos A. Mulan E3 ubiquitin ligase interacts with multiple E2 conjugating enzymes and participates in mitophagy by recruiting GABARAP. Cell Signal. 2014;26:2921-9 pubmed publisher
    ..Our studies identified four different E2 conjugating enzymes (Ube2E2, Ube2E3, Ube2G2 and Ube2L3) as specific interactors of Mulan...
  52. Zhang Q, Fan L, Hou F, Dong A, Wang Y, Tong Y. New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins. Sci Rep. 2015;5:15660 pubmed publisher
    ..We also demonstrated that RNA duplex binding to RC3H2 cross-talks with its E3 ubiquitin ligase function using an in vitro auto-ubiquitination assay. ..
  53. Moynihan T, Ardley H, Nuber U, Rose S, Jones P, Markham A, et al. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 1999;274:30963-8 pubmed
  54. Joch M, Ase A, Chen C, MacDonald P, Kontogiannea M, Corera A, et al. Parkin-mediated monoubiquitination of the PDZ protein PICK1 regulates the activity of acid-sensing ion channels. Mol Biol Cell. 2007;18:3105-18 pubmed
  55. Rajsbaum R, Versteeg G, Schmid S, Maestre A, Belicha Villanueva A, Martínez Romero C, et al. Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response. Immunity. 2014;40:880-95 pubmed publisher
    ..Our work attributes a previously unrecognized activating role of K48-linked unanchored polyubiquitin chains in kinase activation and identifies the UbE2K-TRIM6-ubiquitin axis as critical for IFN signaling and antiviral response. ..
  56. Liu Y, Soetandyo N, Lee J, Liu L, Xu Y, Clemons W, et al. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. elife. 2014;3:e01369 pubmed publisher
    ..DOI: http://dx.doi.org/10.7554/eLife.01369.001. ..
  57. Tiwari S, Weissman A. Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s). J Biol Chem. 2001;276:16193-200 pubmed
    ..These findings also implicate, for the first time, a specific E2 in degradation from the endoplasmic reticulum in mammalian cells. ..
  58. Pabarcus M, Hoe N, Sadeghi S, Patterson C, Wiertz E, Correia M. CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases. Arch Biochem Biophys. 2009;483:66-74 pubmed publisher
    ..the cytosolic domain of the ER-protein gp78, also known as the tumor autocrine motility factor receptor (AMFR), an UBC7-dependent polytopic RING-finger E3, effectively ubiquitinated CYP3A4 in vitro, as did the UbcH5a-dependent ..
  59. Arai R, Yoshikawa S, Murayama K, Imai Y, Takahashi R, Shirouzu M, et al. Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:330-4 pubmed
    The human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7) is involved in protein degradation, including a process known as endoplasmic reticulum-associated degradation (ERAD). The crystal structure of human UBE2G2/UBC7 was solved at 2...