UBE2E2

Summary

Gene Symbol: UBE2E2
Description: ubiquitin conjugating enzyme E2 E2
Alias: UBCH8, ubiquitin-conjugating enzyme E2 E2, E2 ubiquitin-conjugating enzyme E2, ubiquitin carrier protein E2, ubiquitin conjugating enzyme E2E 2, ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast), ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5), ubiquitin-protein ligase E2
Species: human
Products:     UBE2E2

Top Publications

  1. Bekker Jensen S, Rendtlew Danielsen J, Fugger K, Gromova I, Nerstedt A, Lukas C, et al. HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes. Nat Cell Biol. 2010;12:80-6; sup pp 1-12 pubmed publisher
  2. Ito K, Adachi S, Iwakami R, Yasuda H, Muto Y, Seki N, et al. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. Eur J Biochem. 2001;268:2725-32 pubmed
    ..previously cloned cDNAs encoding the N-terminally extended class III human ubiquitin-conjugating enzymes (E2s), UBE2E2 and UBE2E3, the biological functions of which are not known...
  3. Scheper J, Oliva B, Villà Freixa J, Thomson T. Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. Proteins. 2009;74:92-103 pubmed publisher
    ..The application of some of these rules of interaction selectivity has permitted us to experimentally manipulate the selectivity of interaction of the RING-finger domain-E2 pairs under study. ..
  4. Plafker S, Plafker K, Weissman A, Macara I. Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import. J Cell Biol. 2004;167:649-59 pubmed
    ..We now show that the import mechanism for UbcM2 and two other human class III E2s (UbcH6 and UBE2E2) uniquely requires the covalent attachment of Ub to the active site cysteine of these enzymes...
  5. Christensen D, Brzovic P, Klevit R. E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Nat Struct Mol Biol. 2007;14:941-8 pubmed
    ..previously unidentified interactions between the human heterodimeric RING E3 BRCA1-BARD1 and the human E2s UbcH6, Ube2e2, UbcM2, Ubc13, Ube2k and Ube2w...
  6. Zia A, Bhatti A, John P, Kiani A. Data interpretation: deciphering the biological function of Type 2 diabetes associated risk loci. Acta Diabetol. 2015;52:789-800 pubmed publisher
    ..of regulatory function), and only four of these were GWAS significant SNPs (THADA/rs10203174, score = 1b; UBE2E2/rs7612463, score = 2a; ARAP1/rs1552224 and TP53INP1/rs8996852, score = 2b)...
  7. Plengvidhya N, Chanprasert C, Chongjaroen N, Yenchitsomanus P, Homsanit M, Tangjittipokin W. Impact of KCNQ1, CDKN2A/2B, CDKAL1, HHEX, MTNR1B, SLC30A8, TCF7L2, and UBE2E2 on risk of developing type 2 diabetes in Thai population. BMC Med Genet. 2018;19:93 pubmed publisher
    ..Eight SNPs in or near the KCNQ1, CDKN2A/2B, SLC30A8, HHEX, CDKAL1, TCF7L2, MTNR1B, and UBE2E2 genes were genotyped...
  8. Arimoto K, Hishiki T, Kiyonari H, Abe T, Cheng C, Yan M, et al. Murine Herc6 Plays a Critical Role in Protein ISGylation In Vivo and Has an ISGylation-Independent Function in Seminal Vesicles. J Interferon Cytokine Res. 2015;35:351-8 pubmed publisher
    ISG15 conjugation (ISGylation) to proteins is a multistep process involving interferon (IFN)-inducible UBE1L (E1), UbcH8 (E2), and ISG15 E3 ligases (E3s)...
  9. Park J, Yang S, Park J, Ka S, Kim J, Kong Y, et al. Positive feedback regulation of p53 transactivity by DNA damage-induced ISG15 modification. Nat Commun. 2016;7:12513 pubmed publisher
    ..ISG15 has been implicated in the control of tumorigenesis. Intriguingly, the expression of ISG15, UBE1L and UBCH8 is induced by DNA-damaging agents, such as ultraviolet and doxorubicin, which are known to induce p53...

More Information

Publications66

  1. Takenokuchi M, Miyamoto K, Saigo K, Taniguchi T. Bortezomib Causes ER Stress-related Death of Acute Promyelocytic Leukemia Cells Through Excessive Accumulation of PML-RARA. Anticancer Res. 2015;35:3307-16 pubmed
    ..increased the abundance of ubiquitinated-PML-RARA (Ub-PML-RARA), ubiquitin-conjugating human enzyme 8 (UbcH8), and Ub-UbcH8, indicating that UbcH8 is the E2 ubiquitin-conjugating enzyme for PML-RARA...
  2. Kim Y, Kim E, Kim Y, Lee M, Kwon K, Kim K, et al. Consecutive Inhibition of ISG15 Expression and ISGylation by Cytomegalovirus Regulators. PLoS Pathog. 2016;12:e1005850 pubmed publisher
    ..RNA interference of UBE1L (E1), UbcH8 (E2), Herc5 (E3), and UBP43 (ISG15 protease) revealed that ISGylation inhibits HCMV growth by downregulating viral ..
  3. Mizukami S, Yafune A, Watanabe Y, Nakajima K, Jin M, Yoshida T, et al. Identification of epigenetically downregulated Tmem70 and Ube2e2 in rat liver after 28-day treatment with hepatocarcinogenic thioacetamide showing gene product downregulation in hepatocellular preneoplastic and neoplastic lesions produced by tumor pr. Toxicol Lett. 2017;266:13-22 pubmed publisher
    ..Among 47 hypermethylated genes, Hist1h2aa, Tmem70, Ube2e2, and Slk were confirmed to show hypermethylation by methylation-specific quantitative polymerase chain reaction (..
  4. Chen Y, Zhou C, Ji W, Mei Z, Hu B, Zhang W, et al. ELL targets c-Myc for proteasomal degradation and suppresses tumour growth. Nat Commun. 2016;7:11057 pubmed publisher
    ..In addition, we identify that UbcH8 serves as a ubiquitin-conjugating enzyme in this pathway...
  5. Zhou B, Li H, Xing C, Ye H, Feng J, Wu J, et al. Honokiol induces proteasomal degradation of AML1-ETO oncoprotein via increasing ubiquitin conjugase UbcH8 expression in leukemia. Biochem Pharmacol. 2017;128:12-25 pubmed publisher
    ..Mechanistically, HNK obviously increased the expression of UbcH8, an E2-conjugase for the degradation of AML1-ETO, through triggering accumulation of acetylated histones in the ..
  6. Dong C, Gao N, Ross B, Yu F. ISG15 in Host Defense Against Candida albicans Infection in a Mouse Model of Fungal Keratitis. Invest Ophthalmol Vis Sci. 2017;58:2948-2958 pubmed publisher
    ..In vivo, C. albicans infection induced the expression of ISG15, ISGylation-associated genes (UBE1L, UBCH8, and HERC5), and ISGylation in mouse CECs, all of which were enhanced by flagellin-pretreatment...
  7. Burks J, Reed R, Desai S. Free ISG15 triggers an antitumor immune response against breast cancer: a new perspective. Oncotarget. 2015;6:7221-31 pubmed
    ..Breast cancer cells stably expressing control, ISG15, and UbcH8 (ISG15-specific E2 ligase) shRNAs were used to assess the immunoregulatory and antitumor function of free ISG15 in ..
  8. Falvey C, O Donovan T, El Mashed S, Nyhan M, O Reilly S, McKenna S. UBE2L6/UBCH8 and ISG15 attenuate autophagy in esophageal cancer cells. Oncotarget. 2017;8:23479-23491 pubmed publisher
    ..These data identify UBE2L6 and ISG15 as novel inhibitors of autophagy, with the potential to influence chemosensitivity in esophageal cancer cells. ..
  9. Zhou X, Wei J, Chen F, Xiao X, Huang T, He Q, et al. Epigenetic downregulation of the ISG15-conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma. Oncotarget. 2015;6:41077-91 pubmed publisher
    We identified the UBE2L6 gene, encoding the ISG15-conjugating enzyme UbcH8, as one gene significantly downregulated by promoter hypermethylation in nasopharyngeal carcinoma (NPC)...
  10. Haq I, Han Y, Ali T, Wang Y, Gao H, Lin L, et al. Expression of interferon-stimulated gene ISG15 and ubiquitination enzymes is upregulated in peripheral blood monocyte during early pregnancy in dairy cattle. Reprod Biol. 2016;16:255-260 pubmed publisher
    ..protein that mediates the conjugation of different proteins through its ISGylation enzymes UBE1L and UBCH8. We demonstrate that ISG15 and its ISGlyation enzymes UBE1L and UBCH8 are differentially regulated in pregnant and ..
  11. Zhou M, Chen F, Chen H, Wan X, Zhou X, Fang Q, et al. ISG15 inhibits cancer cell growth and promotes apoptosis. Int J Mol Med. 2017;39:446-452 pubmed publisher
    ..levels of ubiquitin-like modifier-activating enzyme 7 (UBA7, also known as UBE1L; ISG15 E1-activating enzyme), UBCH8 (ISG15 E2-conjugating enzyme) and HERC5 (ISG15 E3-ligase) were elevated in the HeLa cells treated with IFN-?...
  12. Moynihan T, Ardley H, Nuber U, Rose S, Jones P, Markham A, et al. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 1999;274:30963-8 pubmed
    ..of these two proteins to UbcH7 is specific insofar that both HHARI and H7-AP1 can bind to the closely related E2, UbcH8, but not to the unrelated E2s UbcH5 and UbcH1...
  13. Chu A, Deng X, Fisher V, Drong A, Zhang Y, Feitosa M, et al. Multiethnic genome-wide meta-analysis of ectopic fat depots identifies loci associated with adipocyte development and differentiation. Nat Genet. 2017;49:125-130 pubmed publisher
    ..In total, we identified seven new loci associated with ectopic-fat traits (ATXN1, UBE2E2, EBF1, RREB1, GSDMB, GRAMD3 and ENSA; P < 5 × 10-8; false discovery rate < 1%)...
  14. Zhang Q, Fan L, Hou F, Dong A, Wang Y, Tong Y. New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins. Sci Rep. 2015;5:15660 pubmed publisher
    ..We also demonstrated that RNA duplex binding to RC3H2 cross-talks with its E3 ubiquitin ligase function using an in vitro auto-ubiquitination assay. ..
  15. Im E, Chung K. Dyrk1A phosphorylates parkin at Ser-131 and negatively regulates its ubiquitin E3 ligase activity. J Neurochem. 2015;134:756-68 pubmed publisher
    ..The present work suggests that parkin phosphorylation by Dyrk1A may affect the pathogenesis of PD under PD-inducing pathological conditions. ..
  16. Zhang Q, Qiao L, Wang X, Ding C, Chen J. UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells. Cell Cycle. 2017;:1-22 pubmed publisher
    ..We demonstrated that UHRF1 down-regulated the expression of UBE2L6 gene that encodes the ISG15-conjugating enzyme UbcH8. Overexpression of UHRF1 reduced UBE2L6 while knockdown UHRF1 elevated the expression of UBE2L6...
  17. Ambivero C, Cilenti L, Main S, Zervos A. Mulan E3 ubiquitin ligase interacts with multiple E2 conjugating enzymes and participates in mitophagy by recruiting GABARAP. Cell Signal. 2014;26:2921-9 pubmed publisher
    ..Our studies identified four different E2 conjugating enzymes (Ube2E2, Ube2E3, Ube2G2 and Ube2L3) as specific interactors of Mulan...
  18. Shibata E, Abbas T, Huang X, Wohlschlegel J, Dutta A. Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. Mol Cell Biol. 2011;31:3136-45 pubmed publisher
    ..b>UBCH8, a member of the UBE2E family of UBCs, ubiquitylates and promotes the degradation of p21, both during the normal ..
  19. Plafker K, Singer J, Plafker S. The ubiquitin conjugating enzyme, UbcM2, engages in novel interactions with components of cullin-3 based E3 ligases. Biochemistry. 2009;48:3527-37 pubmed publisher
    ..Together, these studies represent the first evidence that UbcM2, in concert with substrate adaptors, engages activated CUL3 ligases, thus suggesting that class III E2s are novel regulators of cullin ligases. ..
  20. Tanaka T, Soriano M, Grusby M. SLIM is a nuclear ubiquitin E3 ligase that negatively regulates STAT signaling. Immunity. 2005;22:729-36 pubmed
    ..These studies suggest that SLIM is a novel ubiquitin E3 ligase whose targets include STAT proteins. ..
  21. Ramsey C, Giasson B. Identification and characterization of a novel endogenous murine parkin mutation. J Neurochem. 2010;113:402-17 pubmed publisher
    ..greater tendency to aggregate, exhibited reduced interaction with the E2 ubiquitin-conjugating enzymes, UbcH7 and UbcH8, and demonstrated loss-of-function in promoting the proteosomal degradation of a specific putative substrate, ..
  22. Yamauchi T, Hara K, Maeda S, Yasuda K, Takahashi A, Horikoshi M, et al. A genome-wide association study in the Japanese population identifies susceptibility loci for type 2 diabetes at UBE2E2 and C2CD4A-C2CD4B. Nat Genet. 2010;42:864-8 pubmed publisher
    ..We identified new associations in UBE2E2 on chromosome 3 and in C2CD4A-C2CD4B on chromosome 15 at genome-wide significant levels (rs7612463 in UBE2E2, ..
  23. Kim K, Giannakopoulos N, Virgin H, Zhang D. Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Mol Cell Biol. 2004;24:9592-600 pubmed
    ..This method provides an easy and effective way to identify new targets for the ISGylation system and will facilitate related studies. ..
  24. Arimoto K, Takahashi H, Hishiki T, Konishi H, Fujita T, Shimotohno K. Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125. Proc Natl Acad Sci U S A. 2007;104:7500-5 pubmed
    ..Because RNF125 is enhanced by IFN, these functions constitute a negative regulatory loop circuit for IFN production. ..
  25. Lee J, Wheeler T, Li L, Chin L. Ubiquitination of alpha-synuclein by Siah-1 promotes alpha-synuclein aggregation and apoptotic cell death. Hum Mol Genet. 2008;17:906-17 pubmed
    ..Siah-1 binds the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 and facilitates mono- and di-ubiquitination of alpha-synuclein in vivo...
  26. Li J, Wang J, Yang X, Li J, Qin H, Dong X, et al. The Spen homolog Msx2-interacting nuclear target protein interacts with the E2 ubiquitin-conjugating enzyme UbcH8. Mol Cell Biochem. 2006;288:151-7 pubmed
    ..From a cDNA library of human lymph nodes, a cDNA encoding the ubiquitin-conjugating enzyme UbcH8 was identified...
  27. Takeuchi T, Iwahara S, Saeki Y, Sasajima H, Yokosawa H. Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme. J Biochem. 2005;138:711-9 pubmed
    ..sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1 and E2 enzymes, respectively, for ISG15 conjugation...
  28. Kazakova E, Wu Y, Zhou Z, Chen M, Wang T, Tong H, et al. Association between UBE2E2 variant rs7612463 and type 2 diabetes mellitus in a Chinese Han population. Acta Biochim Pol. 2015;62:241-5 pubmed publisher
    b>UBE2E2 encodes ubiquitin-conjugating enzyme E2E2, which plays an important role in the synthesis and secretion of insulin...
  29. Banka P, Behera A, Sarkar S, Datta A. RING E3-Catalyzed E2 Self-Ubiquitination Attenuates the Activity of Ube2E Ubiquitin-Conjugating Enzymes. J Mol Biol. 2015;427:2290-304 pubmed publisher
    ..Based on these results, we propose that E3-catalyzed self-ubiquitination acts as a key regulatory mechanism that controls the activity of Ube2E class of ubiquitin E2s. ..
  30. Alharbi K, Khan I, Al Sheikh Y, Alharbi F, Alharbi F, Al Nbaheen M. Lack of association between UBE2E2 gene polymorphism (rs7612463) and type 2 diabetes mellitus in a Saudi population. Acta Biochim Pol. 2014;61:769-72 pubmed
    The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells...
  31. Niwa J, Ishigaki S, Doyu M, Suzuki T, Tanaka K, Sobue G. A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Biochem Biophys Res Commun. 2001;281:706-13 pubmed
    ..Dorfin bound specifically with human ubiquitin-conjugating enzymes UbcH7 and UbcH8 through the RING-finger/IBR domain...
  32. Kanazawa T, Ikeda M, Glatt S, Tsutsumi A, Kikuyama H, Kawamura Y, et al. Genome-wide association study of atypical psychosis. Am J Med Genet B Neuropsychiatr Genet. 2013;162B:679-86 pubmed publisher
    ..01), but not BD. This study provides clues about the types of patient whose diagnosis lies between SZ and BD. Studies with larger samples are required to determine the causal variant. ..
  33. Hara K, Fujita H, Johnson T, Yamauchi T, Yasuda K, Horikoshi M, et al. Genome-wide association study identifies three novel loci for type 2 diabetes. Hum Mol Genet. 2014;23:239-46 pubmed publisher
    ..20). This study demonstrates that GWASs based on the imputation of genotypes using modern reference haplotypes such as that from the 1000 Genomes Project data can assist in identification of new loci for common diseases. ..
  34. Aichem A, Pelzer C, Lukasiak S, Kalveram B, Sheppard P, Rani N, et al. USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. Nat Commun. 2010;1:13 pubmed publisher
    ..Interestingly, USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans. ..
  35. Przanowski P, Loska S, Cysewski D, Dabrowski M, Kaminska B. ISG'ylation increases stability of numerous proteins including Stat1, which prevents premature termination of immune response in LPS-stimulated microglia. Neurochem Int. 2018;112:227-233 pubmed publisher
    ..via the sequential action of three enzymes: the E1-activating enzyme UbE1L (UBA7), the E2-conjugating enzyme UBCH8, and E3 ligase HERC5...
  36. Mizukami S, Watanabe Y, Saegusa Y, Nakajima K, Ito Y, Masubuchi Y, et al. Downregulation of UBE2E2 in rat liver cells after hepatocarcinogen treatment facilitates cell proliferation and slowing down of DNA damage response in GST-P-expressing preneoplastic lesions. Toxicol Appl Pharmacol. 2017;334:207-216 pubmed publisher
    We previously found downregulation of ubiquitin-conjugating enzyme E2E 2 (UBE2E2) in GST-P-positive (+) proliferative lesions produced by tumor promotion from early hepatocarcinogenesis stages in rats...
  37. Lakbakbi el Yaagoubi F, Charoute H, Morjane I, Sefri H, Rouba H, Ainahi A, et al. Association analysis of genetic variants with metabolic syndrome components in the Moroccan population. Curr Res Transl Med. 2017;65:121-125 pubmed publisher
    This study aimed to analyze the association between UBE2E2, G6PC2, PROX1, DUSP9, ADCY5 and APOC3 polymorphisms and the risk of metabolic syndrome (MetS) in Moroccan patients...
  38. Tachibana K, Sakurai K, Watanabe M, Miyaso H, Mori C. Associations between changes in the maternal gut microbiome and differentially methylated regions of diabetes-associated genes in fetuses: A pilot study from a birth cohort study. J Diabetes Investig. 2017;8:550-553 pubmed publisher
    ..The present study found that changes in the UBE2E2 and KCNQ1 methylation rates in umbilical cord samples were associated with the proportion of Firmicutes in the ..
  39. Todaro D, Augustus Wallace A, Klein J, Haas A. The mechanism of neural precursor cell expressed developmentally down-regulated 4-2 (Nedd4-2)/NEDD4L-catalyzed polyubiquitin chain assembly. J Biol Chem. 2017;292:19521-19536 pubmed publisher
  40. Kramer O, Müller S, Buchwald M, Reichardt S, Heinzel T. Mechanism for ubiquitylation of the leukemia fusion proteins AML1-ETO and PML-RARalpha. FASEB J. 2008;22:1369-79 pubmed
    ..are degraded by the ubiquitin-proteasome system and that their turnover critically depends on the E2-conjugase UbcH8 and the E3-ligase SIAH-1...
  41. Soss S, Yue Y, Dhe Paganon S, Chazin W. E2 conjugating enzyme selectivity and requirements for function of the E3 ubiquitin ligase CHIP. J Biol Chem. 2011;286:21277-86 pubmed publisher
    ..These data support the proposal that the E2 SPA motif provides specificity for binding to CHIP, whereas activation of the E2?Ub conjugate is derived from other molecular determinants. ..
  42. Lok G, Sy S, Dong S, Ching Y, Tsao S, Thomson T, et al. Differential regulation of RNF8-mediated Lys48- and Lys63-based poly-ubiquitylation. Nucleic Acids Res. 2012;40:196-205 pubmed publisher
    ..ubiquitin ligase RNF8 that plays a critically important role in transducing DNA damage signals, interacts with E2s UBCH8 and UBC13, and catalyzes both K48- and K63-linked ubiquitin chains...
  43. David Y, Ternette N, Edelmann M, Ziv T, Gayer B, Sertchook R, et al. E3 ligases determine ubiquitination site and conjugate type by enforcing specificity on E2 enzymes. J Biol Chem. 2011;286:44104-15 pubmed publisher
    ..Based on several model systems, we propose that although interaction with an E2 is sufficient to promote substrate ubiquitination the E3 molds the reaction into a specific, physiologically relevant protein modification. ..
  44. Christensen D, Klevit R. Dynamic interactions of proteins in complex networks: identifying the complete set of interacting E2s for functional investigation of E3-dependent protein ubiquitination. FEBS J. 2009;276:5381-9 pubmed publisher
    ..Defining the set of E2s that interact with other RING and U-box E3s will open the door for predictive models and lead to a better understand of substrate ubiquitination. ..
  45. Plafker K, Nguyen L, Barneche M, Mirza S, Crawford D, Plafker S. The ubiquitin-conjugating enzyme UbcM2 can regulate the stability and activity of the antioxidant transcription factor Nrf2. J Biol Chem. 2010;285:23064-74 pubmed publisher
    ..This work implicates UbcM2 in the restoration of redox homeostasis following oxidative stress. ..
  46. Eze I, Imboden M, Kumar A, von Eckardstein A, Stolz D, Gerbase M, et al. Air pollution and diabetes association: Modification by type 2 diabetes genetic risk score. Environ Int. 2016;94:263-271 pubmed publisher
    ..We observed similar results with weighted-GRS. Five single variants near GRB14, UBE2E2, PTPRD, VPS26A and KCNQ1 showed nominally significant interactions with PM10 (P<0.05)...
  47. Tokgöz Z, Siepmann T, Streich F, Kumar B, Klein J, Haas A. E1-E2 interactions in ubiquitin and Nedd8 ligation pathways. J Biol Chem. 2012;287:311-21 pubmed publisher
  48. Kim J, Cheong H, Park B, Baik S, Park S, Kim S, et al. Putative association between UBE2E2 polymorphisms and the risk of gestational diabetes mellitus. Gynecol Endocrinol. 2013;29:904-8 pubmed publisher
    We hypothesized that ubiquitin-conjugating enzyme E2 E2 (UBE2E2) may be associated with gestational diabetes mellitus (GDM) and conducted association analyses...
  49. Wheeler T, Chin L, Li Y, Roudabush F, Li L. Regulation of synaptophysin degradation by mammalian homologues of seven in absentia. J Biol Chem. 2002;277:10273-82 pubmed
    ..In addition, Siah proteins interact specifically with the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 and facilitate the ubiquitination of synaptophysin...
  50. Kimura M, Hattori T, Matsuda Y, Yoshioka T, Sumi N, Umeda Y, et al. cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme. Cytogenet Cell Genet. 1997;78:107-11 pubmed
    A cDNA encoding a human ubiquitin-conjugating enzyme (E2) with N-terminal extension (UBE2E2/UbcH8) was isolated...
  51. Shimura H, Hattori N, Kubo S, Mizuno Y, Asakawa S, Minoshima S, et al. Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat Genet. 2000;25:302-5 pubmed
  52. Pruneda J, Smith F, Daurie A, Swaney D, Villen J, Scott J, et al. E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. EMBO J. 2014;33:437-49 pubmed publisher
    ..Mouse oral infection studies indicate that E2~Ub conjugates act as novel regulators of OspG effector kinase function in eukaryotic host cells. ..
  53. Pringa E, Martinez Noel G, Muller U, Harbers K. Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes. J Biol Chem. 2001;276:19617-23 pubmed
    ..Our results establish at the molecular level a link between the U-box and the ubiquitin conjugating system and strongly suggest that proteins containing U-box domains are functionally closely related to RING finger proteins. ..
  54. Xu Z, Kohli E, Devlin K, Bold M, Nix J, Misra S. Interactions between the quality control ubiquitin ligase CHIP and ubiquitin conjugating enzymes. BMC Struct Biol. 2008;8:26 pubmed publisher
    ..CHIP also interacts productively with the class III E2 enzyme Ube2e2, in which the UbcH5- and Ubc13-binding specificity determinants are highly conserved...
  55. Chang Y, Yan X, Xie Y, Gao X, Song A, Zhang D, et al. Different roles for two ubiquitin-like domains of ISG15 in protein modification. J Biol Chem. 2008;283:13370-7 pubmed publisher
    ..The results show that, although the C-terminal domain is sufficient to link ISG15 to UBE1L and UbcH8, the N-terminal domain is dispensable in the activation and transthiolation steps but required for efficient E3-..
  56. Pick E, Lau O, Tsuge T, Menon S, Tong Y, Dohmae N, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol Cell Biol. 2007;27:4708-19 pubmed
    ..Overexpression of DET1 inhibits UV-induced CDT1 degradation in cultured cells. These findings demonstrate that the conserved DET1 complex modulates Cul4A functions by a novel mechanism. ..
  57. Xu K, Jiang L, Zhang M, Zheng X, Gu Y, Wang Z, et al. Type 2 Diabetes Risk Allele UBE2E2 Is Associated With Decreased Glucose-Stimulated Insulin Release in Elderly Chinese Han Individuals. Medicine (Baltimore). 2016;95:e3604 pubmed publisher
    Recently, rs163182 in KCNQ1, rs7612463 in UBE2E2, rs7119 in HMG20A, and rs6815464 in MAEA were discovered as type 2 diabetes (T2D) loci unique to Asians, and rs13342692 in SLC16A11 were newly reported as T2D loci in multiethnicities by ..