U2AF2

Summary

Gene Symbol: U2AF2
Description: U2 small nuclear RNA auxiliary factor 2
Alias: U2AF65, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit, hU2AF65
Species: human
Products:     U2AF2

Top Publications

  1. Chusainow J, Ajuh P, Trinkle Mulcahy L, Sleeman J, Ellenberg J, Lamond A. FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35. RNA. 2005;11:1201-14 pubmed
    We have analyzed the interaction between the U2AF subunits U2AF35 and U2AF65 in vivo using fluorescence resonance energy transfer (FRET) microscopy...
  2. Tavanez J, Madl T, Kooshapur H, Sattler M, Valcarcel J. hnRNP A1 proofreads 3' splice site recognition by U2AF. Mol Cell. 2012;45:314-29 pubmed publisher
    ..Consistent with the functional relevance of this activity for splicing, proofreading assays reveal a role for hnRNP A1 in U2AF-mediated recruitment of U2 snRNP to the pre-mRNA. ..
  3. Zamore P, Green M. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc Natl Acad Sci U S A. 1989;86:9243-7 pubmed
    ..Thus, the simple binding of U2AF initiates mammalian spliceosome assembly by facilitating the ATP-dependent binding of U2 snRNP. ..
  4. Gozani O, Potashkin J, Reed R. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site. Mol Cell Biol. 1998;18:4752-60 pubmed
    ..Together, our data are consistent with a model in which U2AF binds to the pyrimidine tract in the E complex and then interacts with SAP 155 to recruit U2 snRNP to the BPS. ..
  5. Gama Carvalho M, Carvalho M, Kehlenbach A, Valcarcel J, Carmo Fonseca M. Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AF. J Biol Chem. 2001;276:13104-12 pubmed
    ..Shuttling of U2AF between nucleus and cytoplasm possibly represents a means to control the availability of this factor to initiate spliceosome assembly and therefore contribute to regulate splicing. ..
  6. Thickman K, Swenson M, Kabogo J, Gryczynski Z, Kielkopf C. Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors. J Mol Biol. 2006;356:664-83 pubmed
    Essential, protein-protein complexes between the large subunit of the U2 small nuclear RNA auxiliary factor (U2AF65) with the splicing factor 1 (SF1) or the spliceosomal component SF3b155 are exchanged during a critical, ATP-dependent ..
  7. Mackereth C, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, et al. Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature. 2011;475:408-11 pubmed publisher
    ..3'-splice-site-associated polypyrimidine tract RNA by the large subunit of the human U2 snRNP auxiliary factor (U2AF65) as a key early step in pre-mRNA splicing...
  8. Jenkins J, Laird K, Kielkopf C. A Broad range of conformations contribute to the solution ensemble of the essential splicing factor U2AF(65). Biochemistry. 2012;51:5223-5 pubmed
    ..These complementary structural methods demonstrate that diverse splice sites have the opportunity to select compact or extended inter-RRM proximities from the U2AF(65) conformational pool. ..
  9. Zamore P, Patton J, Green M. Cloning and domain structure of the mammalian splicing factor U2AF. Nature. 1992;355:609-14 pubmed
    ..the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro...

More Information

Publications132 found, 100 shown here

  1. Zamore P, Green M. Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution. EMBO J. 1991;10:207-14 pubmed
    ..Our data, in conjunction with those in the accompanying paper [Carmo-Fonseca et al. (1991) EMBO J., 10, 195-206.], suggest that these centers represent novel aspects of nuclear organization. ..
  2. Davies R, Calvio C, Bratt E, Larsson S, Lamond A, Hastie N. WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes. Genes Dev. 1998;12:3217-25 pubmed
    ..considered a transcription factor this study has revealed that WT1 interacts with an essential splicing factor, U2AF65, and associates with the splicing machinery...
  3. Selenko P, Gregorovic G, Sprangers R, Stier G, Rhani Z, Kramer A, et al. Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Mol Cell. 2003;11:965-76 pubmed
    ..This molecular recognition establishes a paradigm for protein binding by a subfamily of noncanonical RRMs. ..
  4. Thickman K, Sickmier E, Kielkopf C. Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif. J Mol Biol. 2007;366:703-10 pubmed
    ..This rare, high-resolution view of an important member of the RRM class of RNA-binding domains highlights the role of alternative side-chain conformations in RNA recognition. ..
  5. Gupta A, Jenkins J, Kielkopf C. RNA induces conformational changes in the SF1/U2AF65 splicing factor complex. J Mol Biol. 2011;405:1128-38 pubmed publisher
    ..We propose that the conformational changes that are induced by assembly of the SF1/U2AF(65)/RNA complex serve to position the pre-mRNA splice site optimally for subsequent stages of splicing. ..
  6. Zhang M, Zamore P, Carmo Fonseca M, Lamond A, Green M. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc Natl Acad Sci U S A. 1992;89:8769-73 pubmed
    ..auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa ..
  7. Tronchere H, Wang J, Fu X. A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA. Nature. 1997;388:397-400 pubmed
    Recognition of a functional 3' splice site in pre-mRNA splicing requires a heterodimer of the proteins U2AF65/U2AF35...
  8. Berglund J, Abovich N, Rosbash M. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes Dev. 1998;12:858-67 pubmed
    ..also binds specifically to branchpoint sequences and interacts with the well studied mammalian splicing factor U2AF65, which binds to the adjacent polypyrimidine (PY) tract...
  9. Jenkins J, Shen H, Green M, Kielkopf C. Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65. J Biol Chem. 2008;283:33641-9 pubmed publisher
    The U2 auxiliary factor large subunit (U2AF65) is an essential pre-mRNA splicing factor for the initial stages of spliceosome assembly...
  10. Chen Y, Zhang L, Jones K. SKIP counteracts p53-mediated apoptosis via selective regulation of p21Cip1 mRNA splicing. Genes Dev. 2011;25:701-16 pubmed publisher
    ..SKIP interacts with the 3' splice site recognition factor U2AF65 and recruits it to the p21(Cip1) gene and mRNA...
  11. JakubauskienÄ— E, Peciuliene I, Vilys L, Mocevicius P, Vilkaitis G, Kanopka A. Gastrointestinal tract tumors and cell lines possess differential splicing factor expression and tumor associated mRNA isoform formation profiles. Cancer Biomark. 2015;15:575-81 pubmed publisher
    ..We present experimental evidence that splicing factor SRSF1, SRSF2, U2AF35, U2AF65 and KHSRP expression levels in gastrointestinal tract (colon, gastric and pancreatic) tumors differ compare to ..
  12. Huang J, Warner L, Sanchez C, Gabel F, Madl T, Mackereth C, et al. Transient electrostatic interactions dominate the conformational equilibrium sampled by multidomain splicing factor U2AF65: a combined NMR and SAXS study. J Am Chem Soc. 2014;136:7068-76 pubmed publisher
    ..Here, we determine the conformational space sampled by the multidomain splicing factor U2AF65 using complementary nuclear magnetic resonance spectroscopy and small-angle scattering data...
  13. Hollander D, Naftelberg S, Lev Maor G, Kornblihtt A, Ast G. How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?. Trends Genet. 2016;32:596-606 pubmed publisher
    ..Also, factors required for SS pairing, such as the U1 small nuclear ribonucleoprotein (snRNP) and U2AF65, associate with RNA polymerase II (RNAPII), while nucleosomes preferentially bind exonic sequences and associate ..
  14. Zuo P, Maniatis T. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes Dev. 1996;10:1356-68 pubmed
    The splicing factor U2AF (U2 snRNP auxiliary factor) is a heterodimer with subunits of 65 and 35 kD (U2AF65 and U2AF35). U2AF65 binds specifically to 3' splice sites, but previous studies failed to demonstrate a function for U2AF35...
  15. Howard J, Lin H, Wallace A, Kim G, Draper J, Haeussler M, et al. HNRNPA1 promotes recognition of splice site decoys by U2AF2 in vivo. Genome Res. 2018;28:689-698 pubmed publisher
    ..events in this process is recognition of the 3' splice site (3'ss) by U2 small nuclear RNA auxiliary factor 2 (U2AF2)...
  16. Whisenant T, Peralta E, Aarreberg L, Gao N, Head S, Ordoukhanian P, et al. The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells. PLoS ONE. 2015;10:e0144409 pubmed publisher
    ..human CD4 T cells and applied this to characterizing activation-induced assembly of splicing factors centered on U2AF2. We immunoprecipitated U2AF2 to identify what mRNA transcripts were bound as a function of activation by TCR ..
  17. Pedrotti S, Busà R, Compagnucci C, Sette C. The RNA recognition motif protein RBM11 is a novel tissue-specific splicing regulator. Nucleic Acids Res. 2012;40:1021-32 pubmed publisher
    ..Thus, our findings identify RBM11 as a novel tissue-specific splicing factor with potential implication in the regulation of alternative splicing during neuron and germ cell differentiation. ..
  18. Sohail M, Xie J. Evolutionary emergence of a novel splice variant with an opposite effect on the cell cycle. Mol Cell Biol. 2015;35:2203-14 pubmed publisher
    ..G tracts inhibit splicing by recruiting heterogeneous nuclear ribonucleoprotein (hnRNP) H and F (H/F) to reduce U2AF65 binding to the Py, causing exon skipping...
  19. Wu T, Fu X. Genomic functions of U2AF in constitutive and regulated splicing. RNA Biol. 2015;12:479-85 pubmed publisher
    ..These findings, coupled with the existence of multiple related proteins to both U2AF65 and U2AF35, beg a series of questions on the universal role of U2AF in functional 3' splice site definition, their ..
  20. Perez Marques F, Simpson P, Yan K, Quasney M, Halligan N, Merchant D, et al. Association of polymorphisms in genes of factors involved in regulation of splicing of cystic fibrosis transmembrane conductance regulator mRNA with acute respiratory distress syndrome in children with pneumonia. Crit Care. 2016;20:281 pubmed publisher
    ..in exon 9 skipping PTB1 (PTBP1), SRp40 (SFRS1), SR2/ASF (SFRS5), TDP-43 (TARDBP), TIA-1 (TIA1), and U2AF(65) (U2AF2) were genotyped. SNPs in the gene of the splicing factor CELF2 (CELF2) were selected by conservation score...
  21. Marchesini M, Ogoti Y, Fiorini E, Aktaş Samur A, Nezi L, D Anca M, et al. ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma. Cancer Cell. 2017;32:88-100.e6 pubmed publisher
    ..resistance to genotoxic agents by modulating YB-1 nuclear localization and interaction with the splicing factor U2AF65, which promotes mRNA processing and the stabilization of transcripts involved in homologous recombination in ..
  22. Sutandy F, Ebersberger S, Huang L, Busch A, Bach M, Kang H, et al. In vitro iCLIP-based modeling uncovers how the splicing factor U2AF2 relies on regulation by cofactors. Genome Res. 2018;28:699-713 pubmed publisher
    ..The RNA-binding protein (RBP) U2AF2 is central to splicing decisions, as it recognizes 3' splice sites and recruits the spliceosome...
  23. Millevoi S, Geraghty F, Idowu B, Tam J, Antoniou M, Vagner S. A novel function for the U2AF 65 splicing factor in promoting pre-mRNA 3'-end processing. EMBO Rep. 2002;3:869-74 pubmed
    ..Therefore, we propose that U2AF 65 promotes 3'-end processing, which contributes to 3'-terminal exon definition. ..
  24. Gama Carvalho M, Barbosa Morais N, Brodsky A, Silver P, Carmo Fonseca M. Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors. Genome Biol. 2006;7:R113 pubmed
    ..the identification of RNAs immunoprecipitated by a monoclonal antibody specific for the U2AF 65 kDa subunit (U2AF65) and demonstrate its association with spliced mRNAs...
  25. Ajuh P, Chusainow J, Ryder U, Lamond A. A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing. EMBO J. 2002;21:6590-602 pubmed
    ..The inhibition of splicing in tsBN67 cells correlates with the temperature-sensitive cell cycle arrest phenotype, suggesting that HCF-1-dependent splicing events may be required for cell cycle progression. ..
  26. Tsoi H, Lau C, Lau K, Chan H. Perturbation of U2AF65/NXF1-mediated RNA nuclear export enhances RNA toxicity in polyQ diseases. Hum Mol Genet. 2011;20:3787-97 pubmed publisher
    ..We found that the human U2AF50 ortholog, U2AF65, interacted directly and specifically with expanded CAG RNA via its RRM3 domain...
  27. Pagliarini V, Pelosi L, Bustamante M, Nobili A, Berardinelli M, D Amelio M, et al. SAM68 is a physiological regulator of SMN2 splicing in spinal muscular atrophy. J Cell Biol. 2015;211:77-90 pubmed publisher
    ..SAM68 binds to SMN2 pre-mRNA, favoring recruitment of the splicing repressor hnRNP A1 and interfering with that of U2AF65 at the 3' splice site of exon 7...
  28. Cretu C, Schmitzová J, Ponce Salvatierra A, Dybkov O, De Laurentiis E, Sharma K, et al. Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations. Mol Cell. 2016;64:307-319 pubmed publisher
    ..Protein-protein crosslinking enabled the localization of the BS-binding proteins p14 and U2AF65 within SF3b155's HEAT-repeat superhelix, which together with SF3b14b forms a composite RNA-binding platform...
  29. Chen L, Weinmeister R, Kralovicova J, Eperon L, Vorechovsky I, Hudson A, et al. Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. Nucleic Acids Res. 2017;45:2051-2067 pubmed publisher
    ..used single molecule methods to test whether the major components implicated in selection, the proteins U2AF35 and U2AF65 and the U2 snRNP, are able to recognize alternative candidate sites or are restricted to one pre-specified site...
  30. Rodríguez Mateo C, Torres B, Gutiérrez G, Pintor Toro J. Downregulation of Lnc-Spry1 mediates TGF-β-induced epithelial-mesenchymal transition by transcriptional and posttranscriptional regulatory mechanisms. Cell Death Differ. 2017;24:785-797 pubmed publisher
    ..Moreover, lnc-Spry1 associates with U2AF65 splicing factor, suggesting a role in alternative splicing...
  31. Ivatt R, Sanchez Martinez A, Godena V, Brown S, Ziviani E, Whitworth A. Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy. Proc Natl Acad Sci U S A. 2014;111:8494-9 pubmed publisher
    ..However, with the recent identification of SREBF1 as a risk locus for sporadic PD, our findings suggest a common mechanistic link between autosomal recessive and sporadic PD, and underscore the importance of mitochondrial homeostasis. ..
  32. Kim J, Chung I. The splicing factor U2AF65 stabilizes TRF1 protein by inhibiting its ubiquitin-dependent proteolysis. Biochem Biophys Res Commun. 2014;443:1124-30 pubmed publisher
    ..Here, we identify U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor 65 (U2AF65), an essentialpre-mRNA splicingfactor, as a novel TRF1-interacting protein...
  33. Lallena M, Martinez C, Valcarcel J, Correas I. Functional association of nuclear protein 4.1 with pre-mRNA splicing factors. J Cell Sci. 1998;111 ( Pt 14):1963-71 pubmed
    ..Based on our combined biochemical and localization results, we propose that 4.1 proteins are part of nuclear structures to which splicing factors functionally associate, most likely for storage purposes. ..
  34. Ganguly B, Kadam N. Mutations of myelodysplastic syndromes (MDS): An update. Mutat Res Rev Mutat Res. 2016;769:47-62 pubmed publisher
    ..Recent discoveries on mutations in RNA-splicing machinery (SF3B1, SRSF2, ZRSR2, U2AF1, U2AF2); DNA methylation (TET2, DNMT3A, IDH1/2); chromatin modification (ASXL1, EZH2); transcription factor (TP53, RUNX1);..
  35. Parra M, Booth B, Weiszmann R, Yee B, Yeo G, Brown J, et al. An important class of intron retention events in human erythroblasts is regulated by cryptic exons proposed to function as splicing decoys. RNA. 2018;24:1255-1265 pubmed publisher
    ..Although many of these cryptic exons were spliced inefficiently, they exhibited substantial binding of U2AF1 and U2AF2 adjacent to their splice acceptor sites...
  36. Palmer K, Tong S, Tuohey L, Cannon P, Ye L, Hannan N, et al. Jumonji Domain Containing Protein 6 Is Decreased in Human Preeclamptic Placentas and Regulates sFLT-1 Splice Variant Production. Biol Reprod. 2016;94:59 pubmed publisher
    ..containing protein 6 (JMJD6) hydroxylates U2 small nuclear ribonucleoprotein auxiliary factor 65-kDa subunit (U2AF65, a component of the splicesome). The hydroxylation by JMJD6 is oxygen dependent...
  37. Ellis J, Llères D, Denegri M, Lamond A, Cáceres J. Spatial mapping of splicing factor complexes involved in exon and intron definition. J Cell Biol. 2008;181:921-34 pubmed publisher
    ..The FLIM data also reveal a previously unknown interaction between HCC1, a factor related to U2AF65, with both subunits of U2AF...
  38. Ji X, Park J, Bahrami Samani E, Lin L, Duncan Lewis C, Pherribo G, et al. αCP binding to a cytosine-rich subset of polypyrimidine tracts drives a novel pathway of cassette exon splicing in the mammalian transcriptome. Nucleic Acids Res. 2016;44:2283-97 pubmed publisher
    ..complex and may be mediated by cooperative interactions with the canonical polypyrimidine tract binding protein, U2AF65. Analysis of αCP-targeted exons predicts a substantial impact on fundamental cell functions...
  39. Pacheco T, Coelho M, Desterro J, Mollet I, Carmo Fonseca M. In vivo requirement of the small subunit of U2AF for recognition of a weak 3' splice site. Mol Cell Biol. 2006;26:8183-90 pubmed
  40. Tian M, Cheng H, Wang Z, Su N, Liu Z, Sun C, et al. Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H. Int J Mol Sci. 2015;16:4209-25 pubmed publisher
    ..revealed that a number of proteins in the spliceosome pathway, such as U2 small nuclear RNA Auxiliary Factor 2 (U2AF2), Eukaryotic Initiation Factor 4A-III (EIF4A3), Cell Division Cycle 5-Like (CDC5L) and Survival Motor Neuron ..
  41. Ladomery M, Slight J, Mc Ghee S, Hastie N. Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)(+) ribonucleoprotein. J Biol Chem. 1999;274:36520-6 pubmed
    ..D. (1995) Cell 81, 391-401); furthermore, WT1 has been shown to interact with the ubiquitous splicing factor U2AF65 (Davies, R. C., Calvo, C., Larsson, S. H., Lamond, A. I., and Hastie, N. D. (1998) Genes Dev...
  42. Wu D, Zhu X, Jimenez Cowell K, Mold A, Sollecito C, Lombana N, et al. Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2. Exp Cell Res. 2015;333:80-92 pubmed publisher
    ..Moreover, DEPDC1B interacted with various signaling molecules such as U2af2, Erh, and Salm...
  43. Chatrikhi R, Wang W, Gupta A, Loerch S, Maucuer A, Kielkopf C. SF1 Phosphorylation Enhances Specific Binding to U2AF65 and Reduces Binding to 3'-Splice-Site RNA. Biophys J. 2016;111:2570-2586 pubmed publisher
  44. Okeyo Owuor T, White B, Chatrikhi R, Mohan D, Kim S, Griffith M, et al. U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing. Leukemia. 2015;29:909-17 pubmed publisher
    ..These effects were not due to impaired U2AF1 (S34F) localization as it co-localized normally with U2AF2 within nuclear speckles...
  45. Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
    ..which were present in separate patient tumors, and shared specificity for a non-mutated self-antigen derived from U2AF2. These results show that the exposed recognition surface of MHC-bound peptides accessible to the TCR contains ..
  46. Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson T, et al. Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60. J Biol Chem. 2009;284:630-9 pubmed publisher
    ..calorimetry, and NMR titrations, we find that Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155...
  47. Soucek S, Zeng Y, Bellur D, Bergkessel M, Morris K, Deng Q, et al. The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA. Mol Cell Biol. 2016;36:2697-2714 pubmed publisher
    ..We validated the interaction between ZC3H14 and U2AF2/U2AF65 Taking all the findings into consideration, we present a model where Nab2/ZC3H14 interacts with ..
  48. Agrawal A, McLaughlin K, Jenkins J, Kielkopf C. Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA. Proc Natl Acad Sci U S A. 2014;111:17420-5 pubmed publisher
    ..We conclude that reduced U2AF(65) binding is a molecular consequence of disease-relevant mutations, and that a structure-guided U2AF(65) variant is capable of manipulating gene expression in eukaryotic cells. ..
  49. Kralovicova J, Knut M, Cross N, Vorechovsky I. Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins. Nucleic Acids Res. 2015;43:3747-63 pubmed publisher
    ..Here, we knocked down U2AF35 and each isoform and characterized transcriptomes of HEK293 cells with varying U2AF35/U2AF65 and U2AF35a/b ratios...
  50. Stepanyuk G, Serrano P, Peralta E, Farr C, Axelrod H, Geralt M, et al. UHM-ULM interactions in the RBM39-U2AF65 splicing-factor complex. Acta Crystallogr D Struct Biol. 2016;72:497-511 pubmed publisher
    ..Here, crystal and solution NMR structures of the RBM39-UHM domain, and the crystal structure of its complex with U2AF65-ULM, are reported...
  51. Fragkouli A, Koukouraki P, Vlachos I, Paraskevopoulou M, Hatzigeorgiou A, Doxakis E. Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP. Sci Rep. 2017;7:44507 pubmed publisher
    ..we report that nELAVLs block APP exon 7 or 8 definition by reducing the binding of the essential splicing factor U2AF65, an effect facilitated by the concurrent binding of AUF-1...
  52. Schor I, Llères D, Risso G, Pawellek A, Ule J, Lamond A, et al. Perturbation of chromatin structure globally affects localization and recruitment of splicing factors. PLoS ONE. 2012;7:e48084 pubmed publisher
    ..To test this, we analyzed the recruitment of the general splicing factor U2AF65 to nascent RNAs by iCLIP technique, as a way to monitor early spliceosome assembly...
  53. Hu S, Wang X, Shan G. Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing. Nat Struct Mol Biol. 2016;23:1011-1019 pubmed publisher
    ..alternative splicing of multiple genes in trans via Alu/anti-Alu pairing with target genes and by interacting with the splicing factor U2AF65. This trans effect of 5S-OT in splicing might be exploited in biotechnological applications.
  54. Wang H, Lin W, Dyck J, Yeakley J, Songyang Z, Cantley L, et al. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998;140:737-50 pubmed
    ..Together, our studies suggest that different SRPK family members may be uniquely regulated and targeted, thereby contributing to splicing regulation in different tissues, during development, or in response to signaling. ..
  55. Tian J, Zhu B, Tian Y, Zhong R, Miao X, Wang L. [Association between pancreatic cancer risk and the interaction of U2AF65 gene polymorphisms and smoking]. Zhonghua Liu Xing Bing Xue Za Zhi. 2014;35:710-3 pubmed
    To determine the association between U2 small nuclear ribonucleoprotein auxiliary factor 35/65 (U2AF35 and U2AF65) and pancreatic cancer (PC). A two-stage analysis case-control study was conducted...
  56. Smith L, Dickinson R, Lucas C, Cousins A, Malygin A, Weldon C, et al. A targeted oligonucleotide enhancer of SMN2 exon 7 splicing forms competing quadruplex and protein complexes in functional conditions. Cell Rep. 2014;9:193-205 pubmed publisher
    ..Complementarity to the 5' end of exon 7 increases U2AF65 binding, but the ESE sequence is required for efficient recruitment of U2 snRNP...
  57. Kralovicova J, Vorechovsky I. Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons. Nucleic Acids Res. 2017;45:417-434 pubmed publisher
    ..The isoform-specific U2AF35 expression was U2AF65-dependent, required interactions between the U2AF-homology motif (UHM) and the ?6 helix of U2AF35, and was fine-..
  58. Sithole N, Williams C, Vaughan A, Kenyon J, Lever A. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol. 2018;430:3111-3128 pubmed publisher
    ..We show that DDX17 interacts with SRSF1/SF2 and the heterodimeric auxiliary factor U2AF65/35, which are essential splicing factors in the generation of Rev and Env/Vpu transcripts.
  59. Agrawal A, Salsi E, Chatrikhi R, Henderson S, Jenkins J, Green M, et al. An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal. Nat Commun. 2016;7:10950 pubmed publisher
    ..Altogether, these results advance the mechanistic understanding of molecular recognition for a major class of splice site signals. ..
  60. David C, Boyne A, MILLHOUSE S, Manley J. The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. Genes Dev. 2011;25:972-83 pubmed publisher
    ..We purified this activity and found that it consists of a complex of two well-known splicing factors: U2AF65 and the Prp19 complex (PRP19C)...
  61. Zhang W, Wu J. Functional properties of p54, a novel SR protein active in constitutive and alternative splicing. Mol Cell Biol. 1996;16:5400-8 pubmed
    ..from those of other SR family members, p54 can directly interact with the 65-kDa subunit of U2 auxiliary factor (U2AF65), a protein associated with the 3' splice site...
  62. Hube F, Ulveling D, Sureau A, Forveille S, Francastel C. Short intron-derived ncRNAs. Nucleic Acids Res. 2017;45:4768-4781 pubmed publisher
    ..their dependence on each key factor in canonical or alternative miRNAs biogenesis (Drosha, DGCR8, DBR1, snRNP70, U2AF65, PRP8, Dicer, Ago2)...
  63. Masuda S, Das R, Cheng H, Hurt E, Dorman N, Reed R. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev. 2005;19:1512-7 pubmed
  64. Ke S, Anquetil V, Zamalloa J, Maity A, Yang A, Arias M, et al. Saturation mutagenesis reveals manifold determinants of exon definition. Genome Res. 2017;: pubmed publisher
    ..of exon-protein complexes between the mutant molecules and proteins associated with spliceosome formation (U2AF35, U2AF65, U1A, and U1-70K) correlated with splicing efficiencies, suggesting exon definition as the step affected by most ..
  65. Lin C, Taggart A, Lim K, Cygan K, Ferraris L, Creton R, et al. RNA structure replaces the need for U2AF2 in splicing. Genome Res. 2016;26:12-23 pubmed publisher
    ..Remarkably, this class of introns does not require U2AF2, a core component of the spliceosome, for its processing...
  66. Kajitani N, Glahder J, Wu C, Yu H, Nilsson K, Schwartz S. hnRNP L controls HPV16 RNA polyadenylation and splicing in an Akt kinase-dependent manner. Nucleic Acids Res. 2017;45:9654-9678 pubmed publisher
    ..This was accompanied by an increased binding of U2AF65 and Sam68 to HPV16 mRNAs. Furthermore, siRNA knock-down of hnRNP L or Akt induced HPV16 gene expression...
  67. Rino J, Desterro J, Pacheco T, Gadella T, Carmo Fonseca M. Splicing factors SF1 and U2AF associate in extraspliceosomal complexes. Mol Cell Biol. 2008;28:3045-57 pubmed publisher
    ..We propose that SF1 and U2AF form extraspliceosomal complexes before and after taking part in the assembly of catalytic spliceosomes. ..
  68. Jenkins J, Agrawal A, Gupta A, Green M, Kielkopf C. U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs. Nucleic Acids Res. 2013;41:3859-73 pubmed publisher
    ..Our results highlight both local and global conformational selection as a means for universal 3' splice site recognition by U2AF(65). ..
  69. Zhang C, Min L, Zhang L, Ma Y, Yang Y, Shou C. Combined analysis identifies six genes correlated with augmented malignancy from non-small cell to small cell lung cancer. Tumour Biol. 2016;37:2193-207 pubmed publisher
    ..Transcript levels of BUB1, E2F1, ESPL1, GTSE1, RAB3B, and U2AF2 were found significantly elevated from normal, ADC, SQC, LCC to SCLC...
  70. Glasser E, Agrawal A, Jenkins J, Kielkopf C. Cancer-Associated Mutations Mapped on High-Resolution Structures of the U2AF2 RNA Recognition Motifs. Biochemistry. 2017;56:4757-4761 pubmed publisher
    ..The U2AF2 splicing factor recognizes polypyrimidine signals that precede most 3' splice sites as a heterodimer with U2AF1...
  71. Corsini L, Bonnal S, Bonna S, Basquin J, Hothorn M, Scheffzek K, et al. U2AF-homology motif interactions are required for alternative splicing regulation by SPF45. Nat Struct Mol Biol. 2007;14:620-9 pubmed
    ..necessary for this activity and binds UHM-ligand motifs (ULMs) present in the 3' splice site-recognizing factors U2AF65, SF1 and SF3b155. We describe a 2.1-A crystal structure of SPF45-UHM in complex with a ULM peptide from SF3b155...
  72. Park C, Park I, Lee S, Sun C, Koh Y, Park S, et al. Genomic dynamics associated with malignant transformation in IDH1 mutated gliomas. Oncotarget. 2015;6:43653-66 pubmed publisher
    ..revealed alterations in the machinery regulating gene expression, including the spliceosome complex (U2AF2), transcription factors (TCF12), and chromatin remodelers (ARID1A)...
  73. Pospiech N, Cibis H, Dietrich L, Müller F, Bange T, Hennig S. Identification of novel PANDAR protein interaction partners involved in splicing regulation. Sci Rep. 2018;8:2798 pubmed publisher
    ..We confirmed potential candidates like U2AF65 and PTBP1, known to be involved in RNA processing...
  74. Kielkopf C, Rodionova N, Green M, Burley S. A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer. Cell. 2001;106:595-605 pubmed
    ..structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution...
  75. Rappsilber J, Ryder U, Lamond A, Mann M. Large-scale proteomic analysis of the human spliceosome. Genome Res. 2002;12:1231-45 pubmed
    ..This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway. ..
  76. Konuma T, Yu D, Zhao C, Ju Y, Sharma R, Ren C, et al. Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4. Sci Rep. 2017;7:16272 pubmed publisher
    ..important roles in regulation of transcription through interactions with transcription regulator BRD4, histones, U2AF65, Luc7L3, and SRSF11...
  77. Dowhan D, Hong E, Auboeuf D, Dennis A, Wilson M, Berget S, et al. Steroid hormone receptor coactivation and alternative RNA splicing by U2AF65-related proteins CAPERalpha and CAPERbeta. Mol Cell. 2005;17:429-39 pubmed
    ..Here, we report that two members of the U2AF65 family of proteins, hCC1...
  78. Huang S, Zhang H, Yu B, McMahon E, Nguyen D, Yu F, et al. Protein 4.1R Exon 16 3' Splice Site Activation Requires Coordination among TIA1, Pcbp1, and RBM39 during Terminal Erythropoiesis. Mol Cell Biol. 2017;37: pubmed publisher
    ..TIA1 and Pcbp1 associate in a complex containing RBM39, which interacts with U2AF65 and SF3b155 and promotes U2 snRNP recruitment to the branch point...
  79. Gault C, Martin F, Mei W, Bai F, Black J, Barbazuk W, et al. Aberrant splicing in maize rough endosperm3 reveals a conserved role for U12 splicing in eukaryotic multicellular development. Proc Natl Acad Sci U S A. 2017;114:E2195-E2204 pubmed publisher
    ..The rgh3 mutant protein disrupts colocalization with a known ZRSR2-interacting protein, U2AF2. These results indicate conserved function for RGH3/ZRSR2 in U12 splicing and a deeply conserved role for the ..
  80. Mang Y, Li L, Ran J, Zhang S, Liu J, Li L, et al. Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells. Onco Targets Ther. 2017;10:1003-1016 pubmed publisher
    ..RNA immunoprecipitation and RNA pull-down assays confirmed that U2AF65 binds to NEAT1...
  81. Ito T, Muto Y, Green M, Yokoyama S. Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65)). EMBO J. 1999;18:4523-34 pubmed
    ..The characteristic alpha1-beta2 structure of the hU2AF(65) RBD1 may interact with other proteins, such as UAP56. ..
  82. Kotlajich M, Hertel K. Death by splicing: tumor suppressor RBM5 freezes splice-site pairing. Mol Cell. 2008;32:162-4 pubmed publisher
    ..In a recent issue of Molecular Cell, Bonnal et al. (2008) demonstrate that the tumor suppressor gene RBM5 regulates alternative splicing of Fas pre-mRNA by interfering with splice-site pairing. ..
  83. Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond A. Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice. EMBO Rep. 2010;11:445-51 pubmed publisher
    ..hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate. ..
  84. Sun X, Li P, Zhu S, Cohen R, Marque L, Ross C, et al. Nuclear retention of full-length HTT RNA is mediated by splicing factors MBNL1 and U2AF65. Sci Rep. 2015;5:12521 pubmed publisher
    ..The splicing and nuclear export factor U2AF65 has the opposite effect, decreasing expHTT nuclear retention and increasing expression of expHTT protein...
  85. Lu Y, Lv J, Fuhrman J, Sun F. Towards enhanced and interpretable clustering/classification in integrative genomics. Nucleic Acids Res. 2017;45:e169 pubmed publisher
    ..by independent studies, including the distribution of the binding sites of IGF2BP and PUM2, the binding competition between hnRNPC and U2AF2, and the intron-exon boundary of U2AF2 [availability: https://github.com/younglululu/Hetero-RP].
  86. Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R. Association of U2 snRNP with the spliceosomal complex E. Nucleic Acids Res. 1997;25:354-61 pubmed
    ..However, we find that U2 snRNP is not as tightly bound in the E complex as it is in the A complex. The possible significance of the weak association of U2 snRNP with the E complex is discussed. ..
  87. Fleckner J, Zhang M, Valcarcel J, Green M. U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction. Genes Dev. 1997;11:1864-72 pubmed
    ..Entry of U2 snRNP into the spliceosome is initiated by interaction of the essential splicing factor U2AF65 with the pre-mRNA polypyrimidine tract...
  88. Kim Y, Kang H, Jetten A. The Krüppel-like zinc finger protein Glis2 functions as a negative modulator of the Wnt/beta-catenin signaling pathway. FEBS Lett. 2007;581:858-64 pubmed
    ..Our results indicate that Glis2 interacts with beta-catenin and suggest that Glis2 functions as a negative modulator of beta-catenin/TCF-mediated transcription. ..
  89. Izquierdo J. Fas splicing regulation during early apoptosis is linked to caspase-mediated cleavage of U2AF65. Mol Biol Cell. 2008;19:3299-307 pubmed publisher
    U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor 65 kDa (U2AF65) is an essential splicing factor in the recognition of the pre-mRNA 3' splice sites during the assembly of the splicing commitment complex...
  90. Rudner D, Kanaar R, Breger K, Rio D. Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo. Mol Cell Biol. 1998;18:1765-73 pubmed
    ..These data indicate that Drosophila U2AF heterodimer formation is essential for viability in vivo, consistent with a requirement for both subunits in splicing in vitro. ..
  91. Peng R, Dye B, Pérez I, Barnard D, Thompson A, Patton J. PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 2002;8:1334-47 pubmed
    ..Sedimentation analyses show that both proteins associate with spliceosomes and with U4/U6.U5 tri-snPNP. ..
  92. Sickmier E, Frato K, Kielkopf C. Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:457-9 pubmed
    The large subunit of the essential pre-mRNA splicing factor U2 auxiliary factor (U2AF65) binds the polypyrimidine tract near the 3' splice site of pre-mRNA introns and directs the association of the U2 small nuclear ribonucleoprotein ..