TTK

Summary

Gene Symbol: TTK
Description: TTK protein kinase
Alias: CT96, ESK, MPH1, MPS1, MPS1L1, PYT, dual specificity protein kinase TTK, cancer/testis antigen 96, monopolar spindle 1 kinase, monopolar spindle 1-like 1, phosphotyrosine picked threonine kinase, phosphotyrosine picked threonine-protein kinase
Species: human
Products:     TTK

Top Publications

  1. Mills G, Schmandt R, McGill M, Amendola A, Hill M, Jacobs K, et al. Expression of TTK, a novel human protein kinase, is associated with cell proliferation. J Biol Chem. 1992;267:16000-6 pubmed
    We have isolated the full-length sequence for a unique human kinase, designated TTK. TTK was initially identified by screening of a T cell expression library with anti-phosphotyrosine antibodies...
  2. Mattison C, Old W, Steiner E, Huneycutt B, Resing K, Ahn N, et al. Mps1 activation loop autophosphorylation enhances kinase activity. J Biol Chem. 2007;282:30553-61 pubmed
    The Mps1 protein kinase is required for proper assembly of the mitotic spindle, checkpoint signaling, and several other aspects of cell growth and differentiation...
  3. Kasbek C, Yang C, Yusof A, Chapman H, Winey M, Fisk H. Preventing the degradation of mps1 at centrosomes is sufficient to cause centrosome reduplication in human cells. Mol Biol Cell. 2007;18:4457-69 pubmed
    ..Cdk2 activity protects the Mps1 protein kinase from proteasome-mediated degradation, and we demonstrate here that Mps1 mediates cyclin A-dependent ..
  4. Hewitt L, Tighe A, Santaguida S, White A, Jones C, Musacchio A, et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol. 2010;190:25-34 pubmed publisher
    b>Mps1 is an essential component of the spindle assembly checkpoint. In this study, we describe a novel Mps1 inhibitor, AZ3146, and use it to probe the role of Mps1's catalytic activity during mitosis...
  5. Santaguida S, Tighe A, D Alise A, Taylor S, Musacchio A. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol. 2010;190:73-87 pubmed publisher
    The catalytic activity of the MPS1 kinase is crucial for the spindle assembly checkpoint and for chromosome biorientation on the mitotic spindle. We report that the small molecule reversine is a potent mitotic inhibitor of MPS1...
  6. Liu S, Chan G, Hittle J, Fujii G, Lees E, Yen T. Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Mol Biol Cell. 2003;14:1638-51 pubmed
    ..determined that the previously identified dual-specificity protein kinase TTK is the human orthologue of the yeast MPS1 kinase. Yeast MPS1 (monopolar spindle) is required for spindle pole duplication and the spindle checkpoint...
  7. Sun T, Yang X, Wang W, Zhang X, Xu Q, Zhu S, et al. Cellular abundance of Mps1 and the role of its carboxyl terminal tail in substrate recruitment. J Biol Chem. 2010;285:38730-9 pubmed publisher
    b>Mps1 is a protein kinase that regulates normal mitotic progression and the spindle checkpoint in response to spindle damage...
  8. Zhang L, Shi R, He C, Cheng C, Song B, Cui H, et al. Oncogenic B-Raf(V600E) abrogates the AKT/B-Raf/Mps1 interaction in melanoma cells. Cancer Lett. 2013;337:125-32 pubmed publisher
    ..Although B-Raf(V600E) induces increased Mps1 protein contributing to centrosome amplification and chromosome instability, the regulatory mechanisms of Mps1 in ..
  9. Tyler R, Chu M, Johnson H, McKenzie E, Gaskell S, Eyers P. Phosphoregulation of human Mps1 kinase. Biochem J. 2009;417:173-81 pubmed publisher
    The dual-specificity protein kinase Mps1 (monopolar spindle 1) is a phosphoprotein required for error-free mitotic progression in eukaryotes...

More Information

Publications74

  1. Thebault P, Chirgadze D, Dou Z, Blundell T, Elowe S, Bolanos Garcia V. Structural and functional insights into the role of the N-terminal Mps1 TPR domain in the SAC (spindle assembly checkpoint). Biochem J. 2012;448:321-8 pubmed publisher
    ..kinetochore targeting of the mitotic kinases Bub1 (budding uninhibited by benzamidine 1), BubR1 (Bub1 related) and Mps1 (monopolar spindle 1), which is mediated by the N-terminus of each kinase. In the present study we report the 1...
  2. Yamagishi Y, Yang C, Tanno Y, Watanabe Y. MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components. Nat Cell Biol. 2012;14:746-52 pubmed publisher
    ..b>MPS1 is an evolutionarily conserved protein kinase required for the SAC and chromosome bi-orientation...
  3. Lan W, Cleveland D. A chemical tool box defines mitotic and interphase roles for Mps1 kinase. J Cell Biol. 2010;190:21-4 pubmed publisher
    ..doi:10.1083/jcb.201001036) use chemical inhibitors to analyze the function of the mitotic checkpoint kinase Mps1. These studies demonstrate that Mps1 kinase activity ensures accurate chromosome segregation through its ..
  4. Bourhis E, Lingel A, Phung Q, Fairbrother W, Cochran A. Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation. Biochemistry. 2009;48:6783-93 pubmed publisher
    ..Interestingly, we found that substitutions at Borealin T230, recently identified as an Mps1 phosphorylation site, can modulate the dimerization state of Borealin...
  5. Yang C, Kasbek C, Majumder S, Yusof A, Fisk H. Mps1 phosphorylation sites regulate the function of centrin 2 in centriole assembly. Mol Biol Cell. 2010;21:4361-72 pubmed publisher
    The nondegradable Mps1(?12/13) protein drives centriole overproduction, suggesting that Mps1 phosphorylates a subset of centrosomal proteins to drive the assembly of new centrioles...
  6. Janssen A, Kops G, Medema R. Targeting the mitotic checkpoint to kill tumor cells. Horm Cancer. 2011;2:113-6 pubmed publisher
    ..by inactivation of the mitotic checkpoint through RNAi-mediated depletion of an essential checkpoint component, Mps1. The mitotic checkpoint delays segregation of chromosomes during mitosis until all chromosomes are properly ..
  7. Dou Z, von Schubert C, Körner R, Santamaria A, Elowe S, Nigg E. Quantitative mass spectrometry analysis reveals similar substrate consensus motif for human Mps1 kinase and Plk1. PLoS ONE. 2011;6:e18793 pubmed publisher
    Members of the Mps1 kinase family play an essential and evolutionarily conserved role in the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis...
  8. Mizukami Y, Kono K, Daigo Y, Takano A, Tsunoda T, Kawaguchi Y, et al. Detection of novel cancer-testis antigen-specific T-cell responses in TIL, regional lymph nodes, and PBL in patients with esophageal squamous cell carcinoma. Cancer Sci. 2008;99:1448-54 pubmed publisher
    We recently identified three HLA-A2402-restricted epitope peptides derived from cancer-testis antigens (CTA), TTK protein kinase (TTK), lymphocyte antigen 6 complex locus K (LY6K), and insulin-like growth factor (IGF)-II mRNA binding ..
  9. Majumder S, Fisk H. VDAC3 and Mps1 negatively regulate ciliogenesis. Cell Cycle. 2013;12:849-58 pubmed publisher
    ..protein that is predominantly associated with the mother centriole and modulates centriole assembly by recruiting Mps1 to centrosomes...
  10. Maire V, Baldeyron C, Richardson M, Tesson B, Vincent Salomon A, Gravier E, et al. TTK/hMPS1 is an attractive therapeutic target for triple-negative breast cancer. PLoS ONE. 2013;8:e63712 pubmed publisher
    ..The data revealed that the human protein kinase monopolar spindle 1 (hMPS1), also known as TTK and involved in mitotic checkpoint, is specifically overexpressed in TNBC, compared to the other BC subgroups and ..
  11. Kanai M, Ma Z, Izumi H, Kim S, Mattison C, Winey M, et al. Physical and functional interaction between mortalin and Mps1 kinase. Genes Cells. 2007;12:797-810 pubmed
    ..Through the search of the kinases that mortalin physically interact with, we identified Mps1 as such a kinase...
  12. Leng M, Chan D, Luo H, Zhu C, Qin J, Wang Y. MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Proc Natl Acad Sci U S A. 2006;103:11485-90 pubmed
    ..BLM is associated with the SAC kinase MPS1 and is phosphorylated at S144 in a MPS1-dependent manner...
  13. Yeh C, Yu Z, Chen P, Cheng Y, Shieh S. Phosphorylation at threonine 288 by cell cycle checkpoint kinase 2 (CHK2) controls human monopolar spindle 1 (Mps1) kinetochore localization. J Biol Chem. 2014;289:15319-27 pubmed publisher
    Human Mps1 (hMps1) is a mitotic checkpoint kinase responsible for sensing the unattached and tensionless kinetochore...
  14. Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed publisher
    ..b>Mps1 phosphorylation of Knl1 results in recruitment of the SAC proteins Bub1, Bub3, and BubR1 to the kinetochore and ..
  15. Lauzé E, Stoelcker B, Luca F, Weiss E, Schutz A, Winey M. Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase. EMBO J. 1995;14:1655-63 pubmed
    The MPS1 gene has been previously identified by a mutant allele that shows defects in spindle pole body (SPB) duplication and cell cycle control...
  16. Petsalaki E, Zachos G. Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments. J Cell Sci. 2013;126:1235-46 pubmed publisher
    ..Decreased Chk1 activity leads to hyper-stable kinetochore microtubules, unstable binding of MCAK, Kif2b and Mps1 to centromeres or kinetochores and reduced phosphorylation of Hec1 by Aurora-B...
  17. Zhang X, Yin Q, Ling Y, Zhang Y, Ma R, Ma Q, et al. Two LXXLL motifs in the N terminus of Mps1 are required for Mps1 nuclear import during G(2)/M transition and sustained spindle checkpoint responses. Cell Cycle. 2011;10:2742-50 pubmed
    Spindle assembly checkpoint kinase Mps1 is spatially and temporally regulated during cell cycle progression...
  18. DANIEL J, Coulter J, Woo J, Wilsbach K, Gabrielson E. High levels of the Mps1 checkpoint protein are protective of aneuploidy in breast cancer cells. Proc Natl Acad Sci U S A. 2011;108:5384-9 pubmed publisher
    ..Noting that aneuploidy in human breast cancer correlates with increased expression levels of the Mps1 checkpoint gene, we investigated whether these high levels of Mps1 contribute to the ability of breast cancer cells ..
  19. Zhu T, Dou Z, Qin B, Jin C, Wang X, Xu L, et al. Phosphorylation of microtubule-binding protein Hec1 by mitotic kinase Aurora B specifies spindle checkpoint kinase Mps1 signaling at the kinetochore. J Biol Chem. 2013;288:36149-59 pubmed publisher
    ..Here, we show that Hec1 interacts with Mps1 and specifies its kinetochore localization via its calponin homology (CH) domain and N-terminal 80 amino acids...
  20. Cui Y, Cheng X, Zhang C, Zhang Y, Li S, Wang C, et al. Degradation of the human mitotic checkpoint kinase Mps1 is cell cycle-regulated by APC-cCdc20 and APC-cCdh1 ubiquitin ligases. J Biol Chem. 2010;285:32988-98 pubmed publisher
    b>Mps1 is a dual specificity protein kinase with key roles in regulating the spindle assembly checkpoint and chromosome-microtubule attachments...
  21. Maciejowski J, George K, Terret M, Zhang C, Shokat K, Jallepalli P. Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling. J Cell Biol. 2010;190:89-100 pubmed publisher
    ..In this study, we use chemical genetics to show that the protein kinase Mps1 regulates both aspects of the SAC...
  22. Niittymäki I, Gylfe A, Laine L, Laakso M, Lehtonen H, Kondelin J, et al. High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle assembly checkpoint. Carcinogenesis. 2011;32:305-11 pubmed publisher
    ..These repeats, located in 258 genes, were initially sequenced in 30 MSI CRC samples. The mitotic checkpoint kinase TTK was found to harbor decay-escaping heterozygous mutations in exon 22 in 59% (105/179) of MSI CRCs, which is notably ..
  23. Huang Y, Chang M, Shieh S. TTK/hMps1 mediates the p53-dependent postmitotic checkpoint by phosphorylating p53 at Thr18. Mol Cell Biol. 2009;29:2935-44 pubmed publisher
    ..evidence that stabilization and activation of p53 by spindle disruption requires the spindle checkpoint kinase TTK/hMps1...
  24. Kasbek C, Yang C, Fisk H. Mps1 as a link between centrosomes and genomic instability. Environ Mol Mutagen. 2009;50:654-65 pubmed publisher
    ..The Mps1 protein kinase is required for centrosome duplication, and preventing the proteasome-dependent degradation of Mps1 ..
  25. Martin Lluesma S, Stucke V, Nigg E. Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science. 2002;297:2267-70 pubmed
    ..We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores...
  26. Wei J, Chou Y, Ou Y, Yeh Y, Tyan S, Sun T, et al. TTK/hMps1 participates in the regulation of DNA damage checkpoint response by phosphorylating CHK2 on threonine 68. J Biol Chem. 2005;280:7748-57 pubmed
    ..Here, we report the identification of the mitotic checkpoint kinase, TTK/hMps1, as a novel CHK2-interacting protein. TTK/hMps1 directly phosphorylates CHK2 on Thr-68 in vitro...
  27. Salvatore G, Nappi T, Salerno P, Jiang Y, Garbi C, Ugolini C, et al. A cell proliferation and chromosomal instability signature in anaplastic thyroid carcinoma. Cancer Res. 2007;67:10148-58 pubmed
    ..protein kinases involved in cell cycle regulation, namely, Polo-like kinase 1 (PLK1) and T cell tyrosine kinase (TTK), are part of the gene set that is up-regulated in ATC...
  28. Tighe A, Staples O, Taylor S. Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores. J Cell Biol. 2008;181:893-901 pubmed publisher
    b>Mps1 is an upstream component of the spindle assembly checkpoint, which, in human cells, is required for checkpoint activation in response to spindle damage but not apparently during an unperturbed mitosis...
  29. Chu M, Chavas L, Douglas K, Eyers P, Tabernero L. Crystal structure of the catalytic domain of the mitotic checkpoint kinase Mps1 in complex with SP600125. J Biol Chem. 2008;283:21495-500 pubmed publisher
    ..Monopolar spindle 1 (Mps1) is a dual-specificity protein kinase that has important roles in the prevention of aneuploidy during the cell ..
  30. Fisk H, Mattison C, Winey M. Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression. Proc Natl Acad Sci U S A. 2003;100:14875-80 pubmed
    ..is essential for the maintenance of genetic stability, and in budding yeast its assembly and function depend on the Mps1 protein kinase. Mps1p is required for centrosome duplication and the spindle checkpoint...
  31. Stucke V, Sillje H, Arnaud L, Nigg E. Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication. EMBO J. 2002;21:1723-32 pubmed
    ..In contrast, centrosome (re-)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication. ..
  32. Sliedrecht T, Zhang C, Shokat K, Kops G. Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel aspects of Mps1 function in mitosis. PLoS ONE. 2010;5:e10251 pubmed publisher
    ..Both processes rely on the mitotic kinase Mps1. We present here two cell lines in which endogenous Mps1 has been stably replaced with a mutant kinase (Mps1-as) ..
  33. Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, et al. A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. J Cell Biol. 2013;201:217-31 pubmed publisher
    ..In this paper, we show that the mitotic checkpoint kinase MPS1 contains an N-terminal localization module, organized in an N-terminal extension (NTE) and a tetratricopeptide ..
  34. Kang J, Chen Y, Zhao Y, Yu H. Autophosphorylation-dependent activation of human Mps1 is required for the spindle checkpoint. Proc Natl Acad Sci U S A. 2007;104:20232-7 pubmed
    ..The protein serine/threonine kinase, Mps1, is a critical component of the spindle checkpoint in human cells and regulates the kinetochore localization of key ..
  35. Jelluma N, Brenkman A, van den Broek N, Cruijsen C, van Osch M, Lens S, et al. Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Cell. 2008;132:233-46 pubmed publisher
    ..Here we show that monopolar spindle 1 (Mps1) kinase, which is essential for the mitotic checkpoint, also controls correction of improper chromosome attachments...
  36. Nihira K, Taira N, Miki Y, Yoshida K. TTK/Mps1 controls nuclear targeting of c-Abl by 14-3-3-coupled phosphorylation in response to oxidative stress. Oncogene. 2008;27:7285-95 pubmed publisher
    ..Here, we identify CLK1, CLK4, MST1, MST2 and TTK (also known as Mps1) as novel Thr735 kinases in vitro by expression cloning strategy using phosphospecific antibody...
  37. Jelluma N, Dansen T, Sliedrecht T, Kwiatkowski N, Kops G. Release of Mps1 from kinetochores is crucial for timely anaphase onset. J Cell Biol. 2010;191:281-90 pubmed publisher
    b>Mps1 kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint...
  38. Saurin A, van der Waal M, Medema R, Lens S, Kops G. Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis. Nat Commun. 2011;2:316 pubmed publisher
    ..In contrast, activity of the kinase Mps1 is indispensible for the mitotic checkpoint...
  39. Yeh Y, Huang Y, Lin T, Shieh S. The cell cycle checkpoint kinase CHK2 mediates DNA damage-induced stabilization of TTK/hMps1. Oncogene. 2009;28:1366-78 pubmed publisher
    ..We have demonstrated previously that, upon DNA damage, TTK/hMps1 activates the checkpoint kinase CHK2 by phosphorylating CHK2 at Thr68...
  40. Cui Y, Guadagno T. B-Raf(V600E) signaling deregulates the mitotic spindle checkpoint through stabilizing Mps1 levels in melanoma cells. Oncogene. 2008;27:3122-33 pubmed
    ..that B-Raf(V600E) signaling deregulates the spindle checkpoint as a consequence of stabilizing monopolar spindle 1 (Mps1) levels in human melanoma cells...
  41. Xu Q, Zhu S, Wang W, Zhang X, Old W, Ahn N, et al. Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Mol Biol Cell. 2009;20:10-20 pubmed publisher
    b>Mps1 is a protein kinase that plays essential roles in spindle checkpoint signaling...
  42. Espeut J, Lara Gonzalez P, Sassine M, Shiau A, Desai A, Abrieu A. Natural Loss of Mps1 Kinase in Nematodes Uncovers a Role for Polo-like Kinase 1 in Spindle Checkpoint Initiation. Cell Rep. 2015;12:58-65 pubmed publisher
    ..b>Mps1 kinase initiates checkpoint signaling by phosphorylating the kinetochore-localized scaffold protein Knl1 to create ..
  43. Cassel R, Bordiga P, Pericat D, Hautefort C, Tighilet B, Chabbert C. New mouse model for inducing and evaluating unilateral vestibular deafferentation syndrome. J Neurosci Methods. 2018;293:128-135 pubmed publisher
    ..peripheral vestibular damages, through transtympanic administration of the glutamate receptors agonist kainate (TTK), and the procedure for evaluating the ensuing clinical signs are detailed...
  44. Gunnar E, Bivik C, Starkenberg A, Thor S. sequoia controls the type I>0 daughter proliferation switch in the developing Drosophila nervous system. Development. 2016;143:3774-3784 pubmed
    ..recently identified Sequoia (Seq), a C2H2 zinc-finger transcription factor with homology to Drosophila Tramtrack (Ttk) and the positive regulatory domain (PRDM) family, as important for lineage progression...
  45. Marquardt J, Perkins J, Beuoy K, Fisk H. Modular elements of the TPR domain in the Mps1 N terminus differentially target Mps1 to the centrosome and kinetochore. Proc Natl Acad Sci U S A. 2016;113:7828-33 pubmed publisher
    ..Here we show that the proper localization of the kinase Mps1 (monopolar spindle 1) is critical to both these processes...
  46. Miao R, Luo H, Zhou H, Li G, Bu D, Yang X, et al. Identification of prognostic biomarkers in hepatitis B virus-related hepatocellular carcinoma and stratification by integrative multi-omics analysis. J Hepatol. 2014;61:840-9 pubmed publisher
    ..The dual-specificity protein kinase TTK, which is a key mitotic checkpoint regulator with links to p53 signaling, was further shown to be a promising ..
  47. Hoover L, Kubalak S. Holding their own: the noncanonical roles of Smad proteins. Sci Signal. 2008;1:pe48 pubmed publisher
    ..Investigating these interactions will likely yield more evidence that Smads serve important and diverse purposes beyond their original reported function as signal transducers in the TGF-beta pathway. ..
  48. Perks M, Warburton J, Bracken L, Reaney S, Emery S, Hirst S. Use of spatially distributed time-integrated sediment sampling networks and distributed fine sediment modelling to inform catchment management. J Environ Manage. 2017;202:469-478 pubmed publisher
    ..In this study of the 362 km2 upland Esk catchment we observe that spatial patterns of sediment flux are consistent over the two year monitoring period ..
  49. Jemaa M, Galluzzi L, Kepp O, Senovilla L, Brands M, Boemer U, et al. Characterization of novel MPS1 inhibitors with preclinical anticancer activity. Cell Death Differ. 2013;20:1532-45 pubmed publisher
    Monopolar spindle 1 (MPS1), a mitotic kinase that is overexpressed in several human cancers, contributes to the alignment of chromosomes to the metaphase plate as well as to the execution of the spindle assembly checkpoint (SAC)...
  50. Miao R, Wu Y, Zhang H, Zhou H, Sun X, Csizmadia E, et al. Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer. Sci Rep. 2016;6:33121 pubmed publisher
    ..including whole genome and transcriptome sequencing), we were able to identify the dual-specific protein kinase TTK as a putative new prognostic biomarker for liver cancer...
  51. Islam M, de Alencar M, da Conceição Machado K, da Conceição Machado K, de Carvalho Melo Cavalcante A, de Sousa D, et al. Phytol in a pharma-medico-stance. Chem Biol Interact. 2015;240:60-73 pubmed publisher
    This study aims to review phytol (PYT), through published articles, periodicals, magazines and patents, which were retrieved from the PM, SD, WS, SP; DII, WIPO, CIPO, USPTO and INPI databases. Among the 149 articles and 62 patents, 27...
  52. Faisal A, Mak G, Gurden M, Xavier C, Anderhub S, Innocenti P, et al. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy. Br J Cancer. 2017;116:1166-1176 pubmed publisher
    ..b>MPS1 kinase activity is essential for the activation of the spindle assembly checkpoint and has been shown to be ..
  53. Wengner A, Siemeister G, Koppitz M, Schulze V, Kosemund D, Klar U, et al. Novel Mps1 Kinase Inhibitors with Potent Antitumor Activity. Mol Cancer Ther. 2016;15:583-92 pubmed publisher
    Monopolar spindle 1 (Mps1) has been shown to function as the key kinase that activates the spindle assembly checkpoint (SAC) to secure proper distribution of chromosomes to daughter cells...
  54. Ji W, Luo Y, Ahmad E, Liu S. Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling. J Biol Chem. 2018;293:484-496 pubmed publisher
    ..MAD1NTD and MAD1CTD also interacted with each other and with the MPS1 protein kinase, which phosphorylated both NTD and CTD...
  55. Lim G, Huh W. Rad52 phosphorylation by Ipl1 and Mps1 contributes to Mps1 kinetochore localization and spindle assembly checkpoint regulation. Proc Natl Acad Sci U S A. 2017;114:E9261-E9270 pubmed publisher
    ..unrelated to homologous recombination in Saccharomyces cerevisiae; it plays a role in the recruitment of Mps1 to the kinetochores and the maintenance of spindle assembly checkpoint (SAC) activity...
  56. Agarwal R, Narayan J, Bhattacharyya A, Saraswat M, Tomar A. Gene expression profiling, pathway analysis and subtype classification reveal molecular heterogeneity in hepatocellular carcinoma and suggest subtype specific therapeutic targets. Cancer Genet. 2017;216-217:37-51 pubmed publisher
    ..In total, 59 kinases associated genes were found over-expressed in HCC, including TTK, MELK, BUB1, NEK2, BUB1B, AURKB, PLK1, CDK1, PKMYT1, PBK, etc...
  57. Huang H, Hittle J, Zappacosta F, Annan R, Hershko A, Yen T. Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol. 2008;183:667-80 pubmed publisher
    ..Finally, RNA interference experiments show that Mps1 is a major but not the exclusive kinase that specifies BubR1 phosphorylation in vivo...
  58. Slee R, Grimes B, Bansal R, Gore J, Blackburn C, Brown L, et al. Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Mol Cancer Ther. 2014;13:307-315 pubmed publisher
    ..Many tumors exhibit upregulation of SAC components such as MPS1, which may help contain CIN within survivable limits...
  59. Kasbek C, Yang C, Fisk H. Antizyme restrains centrosome amplification by regulating the accumulation of Mps1 at centrosomes. Mol Biol Cell. 2010;21:3878-89 pubmed publisher
    ..Because the failure to appropriately degrade the Mps1 protein kinase correlates with centrosome overproduction in tumor-derived cells, defects in the factors that ..
  60. Liang X, Dai Y, Li Z, Gan M, Zhang S, Yin Pan -, et al. Expression and function analysis of mitotic checkpoint genes identifies TTK as a potential therapeutic target for human hepatocellular carcinoma. PLoS ONE. 2014;9:e97739 pubmed publisher
    ..Then we established sorafenib-resistant sublines of HCC cell lines Huh7 and HepG2. Intriguingly, increased TTK expression was significantly associated with acquired sorafenib-resistance in Huh7, HepG2 cells...
  61. Ji Z, Gao H, Jia L, Li B, Yu H. A sequential multi-target Mps1 phosphorylation cascade promotes spindle checkpoint signaling. elife. 2017;6: pubmed publisher
    The master spindle checkpoint kinase Mps1 senses kinetochore-microtubule attachment and promotes checkpoint signaling to ensure accurate chromosome segregation...
  62. Chen S, Wang Y, Ni C, Meng G, Sheng X. HLF/miR-132/TTK axis regulates cell proliferation, metastasis and radiosensitivity of glioma cells. Biomed Pharmacother. 2016;83:898-904 pubmed publisher
    ..invasion assay and clonogenic assay, we investigated the effects of hepatic leukemia factor (HLF), miR-132 and TTK protein kinase (TTK) on cancer cell viability, proliferation, migration, invasion and radiosensitivity...
  63. Liu X, Liao W, Yuan Q, Ou Y, Huang J. TTK activates Akt and promotes proliferation and migration of hepatocellular carcinoma cells. Oncotarget. 2015;6:34309-20 pubmed publisher
    ..The protein kinase human monopolar spindle 1 (hMps1/TTK) gene expression is significantly increased in HCCs...
  64. Zaman G, de Roos J, Libouban M, Prinsen M, de Man J, Buijsman R, et al. TTK Inhibitors as a Targeted Therapy for CTNNB1 (β-catenin) Mutant Cancers. Mol Cancer Ther. 2017;16:2609-2617 pubmed publisher
    The spindle assembly checkpoint kinase TTK (Mps1) is a key regulator of chromosome segregation and is the subject of novel targeted therapy approaches by small-molecule inhibitors...
  65. Kaistha B, Honstein T, Muller V, Bielak S, Sauer M, Kreider R, et al. Key role of dual specificity kinase TTK in proliferation and survival of pancreatic cancer cells. Br J Cancer. 2014;111:1780-7 pubmed publisher
    ..We show that the dual specificity kinase TTK (also known as Mps1), is strongly overexpressed in human PDAC...