TIMM8A

Summary

Gene Symbol: TIMM8A
Description: translocase of inner mitochondrial membrane 8A
Alias: DDP, DDP1, DFN1, MTS, TIM8, mitochondrial import inner membrane translocase subunit Tim8 A, X-linked deafness dystonia protein, deafness dystonia protein 1, deafness/dystonia peptide, translocase of inner mitochondrial membrane 8 homolog A
Species: human
Products:     TIMM8A

Top Publications

  1. Tian M, Liu F, Liu H, Zhang Q, Li L, Hou X, et al. Grape seed procyanidins extract attenuates Cisplatin-induced oxidative stress and testosterone synthase inhibition in rat testes. Syst Biol Reprod Med. 2018;64:246-259 pubmed publisher
    ..assay; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; PAS: periodic acid-Schiff; MTs: Metallothioneins; cAMP: cyclic adenosine monophosphate; cDNA: complementary DNA; RIPA: radioimmunoprecipitation ..
  2. Manna A, De Sarkar S, De S, Bauri A, Chattopadhyay S, Chatterjee M. The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance. Phytomedicine. 2015;22:713-23 pubmed publisher
    ..solid tumor cell lines. The cytotoxicity of MAL-A was evaluated by the MTS-PMS cell viability assay in leukemic cell lines (MOLT3, K562 and HL-60) and compared with solid tumor cell lines (..
  3. Lan X, Zhao C, Chen X, Zhang P, Zang D, Wu J, et al. Nickel pyrithione induces apoptosis in chronic myeloid leukemia cells resistant to imatinib via both Bcr/Abl-dependent and Bcr/Abl-independent mechanisms. J Hematol Oncol. 2016;9:129 pubmed
    ..Cell viability was examined by MTS assay and trypan blue exclusion staining assay in KBM5, KBM5R, K562, BaF3-p210-WT, BaF3-p210-T315I cells, and CML ..
  4. Lee H, Lee M, Lee Y, Choi S, Yang J. Chondrocyte-derived extracellular matrix suppresses pathogenesis of human pterygium epithelial cells by blocking the NF-?B signaling pathways. Mol Vis. 2016;22:1490-1502 pubmed
    ..were measured with the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfenyl)-2H-tetrazolium (MTS) assay. Cell migration was evaluated according to the scratch wound closure assay and the Transwell invasion assay...
  5. Singh N, Lawana V, Luo J, Phong P, Abdalla A, Palanisamy B, et al. Organophosphate pesticide chlorpyrifos impairs STAT1 signaling to induce dopaminergic neurotoxicity: Implications for mitochondria mediated oxidative stress signaling events. Neurobiol Dis. 2018;117:82-113 pubmed publisher
    ..dopaminergic cells) to CPF resulted in a dose-dependent increase in apoptotic cell death as measured by MTS assay and DNA fragmentation...
  6. Suber T, Nikolli I, O Brien M, Londino J, Zhao J, Chen K, et al. FBXO17 promotes cell proliferation through activation of Akt in lung adenocarcinoma cells. Respir Res. 2018;19:206 pubmed publisher
    ..Cell proliferation and viability were analyzed by trypan blue exclusion, BrdU incorporation and an MTS-based fluorometric assay. Studies were also performed after transfecting with sifbxo17...
  7. Wei W, Li Z, Zhu T, Fung H, Wong T, Wen X, et al. Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice. Molecules. 2017;22: pubmed publisher
    ..Their effects on variability of T cells and B cells were determined by using tetrazolium compound (MTS) method...
  8. Yu H, Koilkonda R, Chou T, Porciatti V, Mehta A, Hentall I, et al. Consequences of zygote injection and germline transfer of mutant human mitochondrial DNA in mice. Proc Natl Acad Sci U S A. 2015;112:E5689-98 pubmed publisher
    ..A mitochondria-targeted adeno-associated virus (MTS-AAV) containing the mutant human NADH ubiquinone oxidoreductase subunit 4 (ND4) gene followed by mitochondrial-..
  9. Ye L, Xiang T, Zhu J, Li D, Shao Q, Peng W, et al. Interferon Consensus Sequence-Binding Protein 8, a Tumor Suppressor, Suppresses Tumor Growth and Invasion of Non-Small Cell Lung Cancer by Interacting with the Wnt/β-Catenin Pathway. Cell Physiol Biochem. 2018;51:961-978 pubmed publisher
    ..IRF8 expression were determined by Q- PCR. MSP and A+T determined promotor methylation. MTS, clonogenic, Transwell assay, Flow cytometry, three-dimensional culture and AO/EB stain verified cell function...

More Information

Publications165 found, 100 shown here

  1. Li Y, Gong Y, Ning X, Peng D, Liu L, He S, et al. Downregulation of CLDN7 due to promoter hypermethylation is associated with human clear cell renal cell carcinoma progression and poor prognosis. J Exp Clin Cancer Res. 2018;37:276 pubmed publisher
    ..Biological functions of CLDN7 were investigated by examining cell proliferation using MTS assays and EdU incorporation assays, cell migration by in vitro wound healing assays and transwell migration assays,..
  2. Marouani N, Hallegue D, Sakly M, Benkhalifa M, Ben Rhouma K, Tebourbi O. p,p'-DDT induces testicular oxidative stress-induced apoptosis in adult rats. Reprod Biol Endocrinol. 2017;15:40 pubmed publisher
    ..The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels...
  3. Han S, Lv X, Wang Y, Gong H, Zhang C, Tong A, et al. Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Mol Med Rep. 2015;12:2239-46 pubmed publisher
    ..The results suggested that upregulation of PPAR-γ in the U-87 MG/DDP cells increased cisplatin sensitivity, and the underlying mechanisms included the regulation of MDR and apoptosis ..
  4. Liao Y, Guo Z, Xia X, Liu Y, Huang C, Jiang L, et al. Inhibition of EGFR signaling with Spautin-1 represents a novel therapeutics for prostate cancer. J Exp Clin Cancer Res. 2019;38:157 pubmed publisher
    ..b>MTS, colony formation and Edu staining assays were used to analyze the cell proliferation of PCa cells...
  5. Shen H, Decollogne S, Dilda P, Hau E, Chung S, Luk P, et al. Dual-targeting of aberrant glucose metabolism in glioblastoma. J Exp Clin Cancer Res. 2015;34:14 pubmed publisher
    ..The efficacy of this combinatorial therapy was evaluated by MTS assay, clonogenic surviving assay and apoptotic assays...
  6. Han M, Zhao Y, Tan C, Xiong Y, Wang W, Wu F, et al. Cathepsin L upregulation-induced EMT phenotype is associated with the acquisition of cisplatin or paclitaxel resistance in A549 cells. Acta Pharmacol Sin. 2016;37:1606-1622 pubmed publisher
    ..Human lung cancer A549 cells, A549/PTX (paclitaxel-resistant) cells and A549/DDP (cisplatin-resistant) cells were tested...
  7. Pinkaew D, Changtam C, Tocharus C, Thummayot S, Suksamrarn A, Tocharus J. Di-O-demethylcurcumin protects SK-N-SH cells against mitochondrial and endoplasmic reticulum-mediated apoptotic cell death induced by Aβ25-35. Neurochem Int. 2015;80:110-9 pubmed publisher
    ..was followed by Aβ25-35 (10 µM) in human neuroblastoma SK-N-SH cells improved cell viability by using MTS assay and decreased neuronal cell apoptosis...
  8. Zhang Y, Calado R, Rao M, Hong J, Meeker A, Dumitriu B, et al. Telomerase variant A279T induces telomere dysfunction and inhibits non-canonical telomerase activity in esophageal carcinomas. PLoS ONE. 2014;9:e101010 pubmed publisher
    ..b>MTS, flow cytometry, time lapse microscopy, and murine xenograft techniques were used to assess proliferation, ..
  9. Zhou S, Lu M, Zhao J, Liu S, Li X, Zhang R, et al. The purification of reduced ?2-glycoprotein I showed its native activity in vitro. Lipids Health Dis. 2017;16:173 pubmed publisher
    ..Western blot analysis was used to detect ?2GPI immunoreactivity. MTS and flow cytometry were conducted to investigate its biological effect on oxidative-stress-induced death of human ..
  10. Zheng J, Guo S, Yuan S, Xia H, Zhu Q, Lv Z. Preheating mitigates cadmium toxicity in zebrafish livers: Evidence from promoter demethylation, gene transcription to biochemical levels. Aquat Toxicol. 2017;190:104-111 pubmed publisher
    ..Preheating also mitigated Cd-induced increases in protein and mRNA levels of metallothioneins (MTs), and mRNA levels of several inflammation-related genes...
  11. Zhu P, Jin J, Liao Y, Li J, Yu X, Liao W, et al. A novel prognostic biomarker SPC24 up-regulated in hepatocellular carcinoma. Oncotarget. 2015;6:41383-97 pubmed publisher
    ..Ndc80) kinetochore complex, which plays an essential role in the coupling of kinetochore to spindle microtubules (MTs) and the accurate segregation of chromosomes during mitosis...
  12. Alagrafi F, Alawad A, Abutaha N, Nasr F, Alhazzaa O, Alharbi S, et al. In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta. BMC Complement Altern Med. 2017;17:342 pubmed publisher
    ..Cytotoxicity was evaluated by an MTS cell viability assay...
  13. Clemens M, Kennon McGill S, Apte U, James L, Finck B, McGill M. The inhibitor of glycerol 3-phosphate acyltransferase FSG67 blunts liver regeneration after acetaminophen overdose by altering GSK3β and Wnt/β-catenin signaling. Food Chem Toxicol. 2019;125:279-288 pubmed publisher
    ..Some mice also received the GSK3 inhibitor L803-mts. Blood and liver were collected at multiple time points...
  14. Kolosov D, Donly C, MacMillan H, O Donnell M. Transcriptomic analysis of the Malpighian tubules of Trichoplusia ni: Clues to mechanisms for switching from ion secretion to ion reabsorption in the distal ileac plexus. J Insect Physiol. 2019;112:73-89 pubmed publisher
    Excretion of metabolic wastes and toxins in insect Malpighian tubules (MTs) is coupled to secretion of ions and fluid...
  15. Li R, Zhuang A, Ma J, Ji L, Hou X, Chen H, et al. Effect of Jieduquyuziyin prescription-treated rat serum on MeCP2 gene expression in Jurkat T cells. In Vitro Cell Dev Biol Anim. 2018;54:692-704 pubmed publisher
    ..3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays were used to screen the optimal concentration of drug-containing serum...
  16. Yoshimoto T, Fujita T, Kajiya M, Matsuda S, Ouhara K, Shiba H, et al. Involvement of smad2 and Erk/Akt cascade in TGF-β1-induced apoptosis in human gingival epithelial cells. Cytokine. 2015;75:165-73 pubmed publisher
    ..Surviving and apoptotic cells were detected using an MTS assay and TUNEL staining, respectively...
  17. Bebianno M, Gonzalez Rey M, Gomes T, Mattos J, Flores Nunes F, Bainy A. Is gene transcription in mussel gills altered after exposure to Ag nanoparticles?. Environ Sci Pollut Res Int. 2015;22:17425-33 pubmed publisher
    ..that enzyme kinetics and relative abundance of proteins (increase of antioxidant enzymes and metalllothioneins (MTs) with the time of exposure) do not always directly reflect their relative mRNA levels...
  18. Zong M, Lu T, Fan S, Zhang H, Gong R, Sun L, et al. Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther. 2015;17:100 pubmed publisher
    ..Doxorubicin (Adriamycin, ADR) was used to induce apoptosis. Cell proliferation was determined by MTS assay. Flow cytometry was used to detect cell cycle and apoptosis...
  19. Wu Y, Bi Y, Tan Z, Xie L, Xu H, Fan H, et al. Tanshinone I inhibits vascular smooth muscle cell proliferation by targeting insulin-like growth factor-1 receptor/phosphatidylinositol-3-kinase signaling pathway. Eur J Pharmacol. 2019;853:93-102 pubmed publisher
    ..manner, based on 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and 5-ethynyl-2'-deoxyuridine (EdU) assays...
  20. Huang X, Chen L, Liu W, Qiao Q, Wu K, Wen J, et al. Involvement of oxidative stress and cytoskeletal disruption in microcystin-induced apoptosis in CIK cells. Aquat Toxicol. 2015;165:41-50 pubmed publisher
    ..scanning confocal microscope observation showed aggregation and collapse of microfilaments (MFs) and microtubules (MTs) in CIK cells, and even loss of some cytoskeleton structure...
  21. Guo S, Zheng J, Yuan S, Zhu Q. Effects of heat and cadmium exposure on stress-related responses in the liver of female zebrafish: Heat increases cadmium toxicity. Sci Total Environ. 2018;618:1363-1370 pubmed publisher
    ..down-regulated Zir-, Irt-like protein 8 (ZIP8) and copper transporter 1 (CTR1) and up-regulated metallothioneins (MTs) in Cd-exposed fish, possibly suggesting Cu and Zn depletion and Cd accumulation...
  22. Zhao K, Wen L. DMF attenuates cisplatin-induced kidney injury via activating Nrf2 signaling pathway and inhibiting NF-kB signaling pathway. Eur Rev Med Pharmacol Sci. 2018;22:8924-8931 pubmed publisher
    ..A total of 30 Sprague Dawley (SD) rats were randomly assigned into sham group, cisplatin treatment group (DDP group) and DMF + cisplatin treatment group (DMF group), with 10 rats in each group...
  23. Zou Y, Yang X, Yuan S, Zhang P, Ye Y, Li Y. Downregulation of dickkopf-1 enhances the proliferation and osteogenic potential of fibroblasts isolated from ankylosing spondylitis patients via the Wnt/?-catenin signaling pathway in vitro. Connect Tissue Res. 2016;57:200-11 pubmed publisher
    ..b>MTS [(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl) 2-(4-sulfophenyl)-2H-tetrazolium] and a 5-ethynyl-2'-..
  24. Ma M, Zhao L, Sun G, Zhang C, Liu L, Du Y, et al. Mda-7/IL-24 enhances sensitivity of B cell lymphoma to chemotherapy drugs. Oncol Rep. 2016;35:3122-30 pubmed publisher
    ..Raji and Daudi cells to cis-diamminedichloroplatinum (CDDP), epirubicin and vinblastine (VCR) were assessed by the MTS method, and the IC50 value calculated...
  25. Tang E, Mok K, Lee W, Cheng C. EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study. Endocrinology. 2015;156:680-93 pubmed publisher
    ..Studies propose that because microtubules (MTs) serve as the tracks for transporting cell organelles, they may also serve a similar function in the transport of ..
  26. Rong L, Maneerung T, Ng J, Neoh K, Bay B, Tong Y, et al. Co-gasification of sewage sludge and woody biomass in a fixed-bed downdraft gasifier: toxicity assessment of solid residues. Waste Manag. 2015;36:241-55 pubmed publisher
    ..From the analyses of cell viability (using MTS assay) and morphology (using fluorescence microscope), the high toxicity of the gasification bottom ash extract ..
  27. Chen C, Xu C, Zhou T, Gao B, Zhou H, Chen C, et al. Abnormal osteogenic and chondrogenic differentiation of human mesenchymal stem cells from patients with adolescent idiopathic scoliosis in response to melatonin. Mol Med Rep. 2016;14:1201-9 pubmed publisher
    ..The expression levels of melatonin receptors (MTs) 1 and 2 were detected by western blotting...
  28. Zhang L, Zhang Z. Sestrin2 aggravates oxidative stress of neurons by decreasing the expression of Nrf2. Eur Rev Med Pharmacol Sci. 2018;22:3493-3501 pubmed publisher
    ..Oxygen-glucose deprivation/reoxygenation (OGD/R) model of primary neurons was established. MTS and LDH (lactate dehydrogenase) kit were used to detect cell viability and cell damage by colorimetric method...
  29. Bailon Moscoso N, Tinitana F, Martínez Espinosa R, Jaramillo Velez A, Palacio Arpi A, Aguilar Hernandez J, et al. Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador. BMC Complement Altern Med. 2017;17:539 pubmed publisher
    ..cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay...
  30. Ito H, Ichiyanagi O, Naito S, Bilim V, Tomita Y, Kato T, et al. GSK-3 directly regulates phospho-4EBP1 in renal cell carcinoma cell-line: an intrinsic subcellular mechanism for resistance to mTORC1 inhibition. BMC Cancer. 2016;16:393 pubmed publisher
    ..Cell viability, cell cycling and direct interaction between GSK-3? and 4EBP1 were evaluated with MTS assay, flowcytometry and in vitro kinase assay with recombinant GSK-3? and 4EBP1products, respectively...
  31. Liao Y, Jiang Y, He H, Ni H, Tu Z, Zhang S, et al. NEDD8-mediated neddylation is required for human endometrial stromal proliferation and decidualization. Hum Reprod. 2015;30:1665-76 pubmed publisher
    ..An MTS assay was performed to evaluate the effects of neddylation inhibition by MLN4924 on HESC proliferation...
  32. Yin K, Zhu R, Wang S, Zhao R. Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells. Stem Cells Dev. 2017;26:762-775 pubmed publisher
    ..In this study, cell viability was assessed using MTS assays and cell cycle was evaluated by fluorescence-activated cell sorting (FACS)...
  33. Cinar O, Semiz O, Can A. Carbofuran alters centrosome and spindle organization, and delays cell division in oocytes and mitotic cells. Toxicol Sci. 2015;144:298-306 pubmed publisher
    ..Partial loss of spindle microtubules (MTs) was noted, which consequently gave rise to a diamond shape spindle...
  34. Dong Y, Wang Z, Xie G, Li C, Zuo W, Meng G, et al. Pregnane X receptor is associated with unfavorable survival and induces chemotherapeutic resistance by transcriptional activating multidrug resistance-related protein 3 in colorectal cancer. Mol Cancer. 2017;16:71 pubmed publisher
    ..The roles of PXR on cancer cell proliferation, apoptosis and tumor growth with L-OHP-treated were investigated by MTS, colony formation, flow cytometry and xenograft tumor assays...
  35. Boehlke C, Janusch H, Hamann C, Powelske C, Mergen M, Herbst H, et al. A Cilia Independent Role of Ift88/Polaris during Cell Migration. PLoS ONE. 2015;10:e0140378 pubmed publisher
    ..Ift88 is required for the polarization of migrating MDCK cells, and Ift88 depleted cells have fewer MTs at the leading edge. Neither MT dynamics nor MT nucleation are dependent on Ift88...
  36. Miller J, Herda T, Trevino M, Mosier E. The effects of passive stretching plus vibration on strength and activation of the plantar flexors. Appl Physiol Nutr Metab. 2016;41:917-23 pubmed publisher
    ..were assessed pre- and post-passive stretching via PROM, passive torque (PASSTQ), and musculotendinous stiffness (MTS) measurements...
  37. Boukadida K, Cachot J, Clérandeaux C, Gourves P, Banni M. Early and efficient induction of antioxidant defense system in Mytilus galloprovincialis embryos exposed to metals and heat stress. Ecotoxicol Environ Saf. 2017;138:105-112 pubmed publisher
    ..At 22°C, a significant decrease in mRNA abundance of cat, gst and sod and a significant up-regulation of mts targets (mt10 and mt20) were observed...
  38. Arafat W, Zhou T, Naoum G, Buchsbaum D. Targeted radiotherapy potentiates the cytotoxicity of a novel anti-human DR5 monoclonal antibody and the adenovirus encoding soluble TRAIL in prostate cancer. J Egypt Natl Canc Inst. 2015;27:205-15 pubmed publisher
    ..Cell survival using the MTS assay and colony forming assay were used to determine radiosensitization...
  39. Donatella N, Marcello B, Gaetano V, Massimo P, Massimo M, Giancarlo B. What the Young Physician Should Know About May-Thurner Syndrome. Transl Med UniSa. 2015;12:19-28 pubmed
    May-Thurner syndrome (MTS) is an anatomically variable condition resulting in compression of the left common iliac vein between the right common iliac artery and the underlying spine with subsequent development of a left deep vein ..
  40. Fraveto A, Cardinale V, Bragazzi M, Giuliante F, De Rose A, Grazi G, et al. Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures. PLoS ONE. 2015;10:e0142124 pubmed publisher
    ..18 IHCCA patients) and evaluated for cell proliferation (MTS assay) and apoptosis (Caspase 3) after incubation (72 hours) with increasing concentrations of different drugs...
  41. Ji S, Li R, Wang Q, Miao W, Li Z, Si L, et al. Anti-H1N1 virus, cytotoxic and Nrf2 activation activities of chemical constituents from Scutellaria baicalensis. J Ethnopharmacol. 2015;176:475-84 pubmed publisher
    ..A/WSN/33 (H1N1) virus in MDCK cells, cytotoxic activities against HepG2, SW480 and MCF7 human cancer cells by MTS assay, and antioxidant activities by Nrf2 luciferase reporter assay...
  42. Jiang Y, Zhang L, Rupasinghe H. Antiproliferative effects of extracts from Salvia officinalis L. and Saliva miltiorrhiza Bunge on hepatocellular carcinoma cells. Biomed Pharmacother. 2017;85:57-67 pubmed publisher
    ..The cytotoxicity and anti-proliferative abilities of the extracts were evaluated by measuring cell viability (MTS assay), lactate dehydrogenase (LDH) leakage, the cellular ATP level, morphological changes using an inverted ..
  43. Baratéla F, Higa O, Dos Passos E, de Queiroz A. Fabrication of electrospun HPGL scaffolds via glycidyl methacrylate cross-linker: Morphology, mechanical and biological properties. Mater Sci Eng C Mater Biol Appl. 2017;73:72-79 pubmed publisher
    ..4±11.32kPa), which is approximately 58 times higher than that of non-crosslinked EHPGLS (5.1±2.12kPa). The MTS cell viability results showed that the EHPGLS have no significant cytotoxicity effect on Chinese hamster ovary (CHO-..
  44. Song J, Yin J, Bai Z, Zhang J, Meng H, Cai J, et al. The Profile of Serum microRNAs Predicts Prognosis for Resected Gastric Cancer Patients Receiving Platinum-Based Chemotherapy. Dig Dis Sci. 2017;62:1223-1234 pubmed publisher
    ..Microarrays were used to screen cisplatin-resistant SGC7901/DDP GC cells and the parental SGC7901 cell lines for miRNAs related to chemotherapy sensitivity...
  45. Ireland S, Cross R, Decker K, Mitra B. Perceptions of an educational programme for registered nurses who work at non-major trauma services in Victoria, Australia: The Nursing Emergency eXternal Trauma Programme. Australas Emerg Nurs J. 2017;20:131-138 pubmed publisher
    Emergency nurses working in non-Major Trauma Service (non-MTS) facilities face the challenge of providing immediate care to seriously injured patients, despite infrequent presentations at their workplace...
  46. Subhash V, Tan S, Tan W, Yeo M, Xie C, Wong F, et al. GTSE1 expression represses apoptotic signaling and confers cisplatin resistance in gastric cancer cells. BMC Cancer. 2015;15:550 pubmed publisher
    ..was knocked-down using shRNA's and its effects on cisplatin cytotoxicity and cell survival were detected by MTS, proliferation and clonogenic survival assays...
  47. Mitra A, Ruhnow F, Nitzsche B, Diez S. Impact-Free Measurement of Microtubule Rotations on Kinesin and Cytoplasmic-Dynein Coated Surfaces. PLoS ONE. 2015;10:e0136920 pubmed publisher
    Knowledge about the three-dimensional stepping of motor proteins on the surface of microtubules (MTs) as well as the torsional components in their power strokes can be inferred from longitudinal MT rotations in gliding motility assays...
  48. Sawada K, Fujioka Kobayashi M, Kobayashi E, Schaller B, Miron R. Effects of Antiseptic Solutions Commonly Used in Dentistry on Bone Viability, Bone Morphology, and Release of Growth Factors. J Oral Maxillofac Surg. 2016;74:247-54 pubmed publisher
    ..cortical bone were rinsed in the antiseptic solutions for 10 minutes and assessed for cell viability using an MTS assay and protein release of transforming growth factor (TGF-β1), bone morphogenetic protein 2 (BMP2), vascular ..
  49. Yokoyama H. Chromatin-Binding Proteins Moonlight as Mitotic Microtubule Regulators. Trends Cell Biol. 2016;26:161-4 pubmed publisher
    Some microtubule (MT)-associated proteins bind to MTs and chromatin simultaneously to fulfill their mitotic spindle function...
  50. Weng Q, Ren L, Guo L, Hu Y, Zhou H, Bao Y, et al. Curcumin analogue, A13, exhibits anti-leukemia effect via inhibiting STAT3. Tumour Biol. 2016;37:9959-66 pubmed publisher
    ..was able to reduce the viability of mastocytoma (P815 cells) and reticulum cell sarcoma (A20 cells) as measured by MTS assay...
  51. Wang Z, Feng C, Ye C, Wang Y, Yan C, Li R, et al. Subcellular partitioning profiles and metallothionein levels in indigenous clams Moerella iridescens from a metal-impacted coastal bay. Aquat Toxicol. 2016;176:10-23 pubmed publisher
    ..and subcellular distribution of metals in the digestive gland of clams with special emphasis on metallothioneins (MTs) was investigated...
  52. Melkov A, Abdu U. Regulation of long-distance transport of mitochondria along microtubules. Cell Mol Life Sci. 2018;75:163-176 pubmed publisher
    ..Tight coupling between MTs, motors, and the mitochondria is needed for the organelle precise localization...
  53. Xiao H, Xiao W, Cao J, Li H, Guan W, Guo X, et al. miR-206 functions as a novel cell cycle regulator and tumor suppressor in clear-cell renal cell carcinoma. Cancer Lett. 2016;374:107-116 pubmed publisher
    In this study we tried to systematically investigate the tumor suppressing microRNAs in ccRCC. The MTS cell viability and colony formation assay were used to systematically detect the tumor suppressing ability of down-regulated miRNAs in ..
  54. Perić L, Nerlović V, Žurga P, Žilić L, Ramsak A. Variations of biomarkers response in mussels Mytilus galloprovincialis to low, moderate and high concentrations of organic chemicals and metals. Chemosphere. 2017;174:554-562 pubmed publisher
    The changes of acetylcholinesterase activity (AChE), metallothioneins content (MTs), catalase activity (CAT) and lipid peroxidation (LPO) were assessed after 4 days exposure of mussels Mytilus galloprovincialis to a wide range of ..
  55. Jia X, Zhang Z, Luo K, Zheng G, Lu M, Song Y, et al. TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer. Sci Rep. 2017;7:3744 pubmed publisher
    ..in multidrug resistant tongue cancer cells (Tca8113/PYM) and cisplatin-resistant A549 lung cancer cells (A549/DDP) was accompanied by c-Myc upregulation, compared to respective parental cells...
  56. Giménez C, Locatelli P, Montini Ballarin F, Orlowski A, Dewey R, Peña M, et al. Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration. Cytotechnology. 2018;70:651-664 pubmed publisher
    ..ovine diaphragm biopsies were characterized by immunohistochemistry and ability to differentiate into myotubes (MTs) and transduced with a lentiviral vector encoding cx43...
  57. Bu H, Song X, Xia J. The hydrogen and oxygen isotopic compositions of precipitation in a forested watershed of the South Qinling Mts., China. Environ Sci Pollut Res Int. 2018;25:6720-6728 pubmed publisher
    ..were firstly investigated from May 2012 to November 2013 in the Jinshui River basin of the South Qinling Mts., China...
  58. Chang C, Chen Y, Quintanilla C, Hsieh T, Liou J. EB1 binding restricts STIM1 translocation to ER-PM junctions and regulates store-operated Ca2+ entry. J Cell Biol. 2018;217:2047-2058 pubmed publisher
    ..that STIM1-EB1 interaction shapes the kinetics and amplitude of local SOCE in cellular regions with growing MTs and contributes to spatiotemporal regulation of Ca2+ signaling crucial for cellular functions and ..
  59. Shin Y, Du Y, Collier S, Ohi M, Lang M, Ohi R. Biased Brownian motion as a mechanism to facilitate nanometer-scale exploration of the microtubule plus end by a kinesin-8. Proc Natl Acad Sci U S A. 2015;112:E3826-35 pubmed publisher
    ..combined with their plus-end motility cause Kip3 and Kif18A to enrich preferentially at the plus ends of long MTs, promoting MT catastrophes or pausing...
  60. Kowald G, Stürzenbaum S, Blindauer C. Earthworm Lumbricus rubellus MT-2: Metal Binding and Protein Folding of a True Cadmium-MT. Int J Mol Sci. 2016;17: pubmed publisher
    Earthworms express, as most animals, metallothioneins (MTs)-small, cysteine-rich proteins that bind d(10) metal ions (Zn(II), Cd(II), or Cu(I)) in clusters...
  61. Gersey Z, Rodriguez G, Barbarite E, Sanchez A, Walters W, Ohaeto K, et al. Curcumin decreases malignant characteristics of glioblastoma stem cells via induction of reactive oxygen species. BMC Cancer. 2017;17:99 pubmed publisher
    ..Cell viability was determined with MTS assay. GSC proliferation, sphere forming and colony forming assays were conducted through standard counting methods...
  62. Park K, Villar M, Artigues A, Lutkenhaus J. MinE conformational dynamics regulate membrane binding, MinD interaction, and Min oscillation. Proc Natl Acad Sci U S A. 2017;114:7497-7504 pubmed publisher
    ..suppressors of this mutant suggests it has difficulty in releasing the N-terminal membrane targeting sequences (MTS)...
  63. Mary Photini S, Chaiwangyen W, Weber M, Al Kawlani B, Favaro R, Jeschke U, et al. PIM kinases 1, 2 and 3 in intracellular LIF signaling, proliferation and apoptosis in trophoblastic cells. Exp Cell Res. 2017;359:275-283 pubmed publisher
    ..Functions were evaluated by PIM inhibition followed by analysis of kinetics of cell viability as assessed by MTS assay, proliferation by BrdU assay, and apoptosis by Western blotting for BAD, BCL-XL, (cleaved) PARP, CASP3 and c-..
  64. Kurup N, Li Y, Goncharov A, Jin Y. Intermediate filament accumulation can stabilize microtubules in Caenorhabditis elegans motor neurons. Proc Natl Acad Sci U S A. 2018;115:3114-3119 pubmed publisher
    ..synapses of motor neurons are stereotypically rewired through a process facilitated by dynamic microtubules (MTs)...
  65. Liu S, Yang H, Chen Y, He B, Chen Q. Krüppel-Like Factor 4 Enhances Sensitivity of Cisplatin to Lung Cancer Cells and Inhibits Regulating Epithelial-to-Mesenchymal Transition. Oncol Res. 2016;24:81-7 pubmed publisher
    ..We developed cisplatin-resistant lung cancer cell line A549/DDP, and then a battery of experiments was used to analyze the effects of KLF4 in cisplatin resistance of lung cancer...
  66. Shin H, Kim K, Kim B, Lee J, Park Y, Lee J, et al. Benign enhancing components of mature ovarian teratoma: magnetic resonance imaging features and pathologic correlation. Clin Imaging. 2016;40:1156-1161 pubmed publisher
    ..Enhancing solid components were observed in 24 (18.8%) of 128 benign MTs. The largest diameter ranged from 5.9 to 42.2 mm. The appearance was variable. Of 24 tumors, 19 (79...
  67. Backes S, Hess S, Boos F, Woellhaf M, Gödel S, Jung M, et al. Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences. J Cell Biol. 2018;217:1369-1382 pubmed publisher
    ..In this study, we show that many preproteins contain additional internal MTS-like signals (iMTS-Ls) in their mature region that share the characteristic properties of presequences...
  68. Zhang C, Zhao L, Wu H, Tian G, Dai S, Zhao R, et al. C/D-Box Snord105b Promotes Tumorigenesis in Gastric Cancer via ALDOA/C-Myc Pathway. Cell Physiol Biochem. 2018;45:2471-2482 pubmed publisher
    ..Cell viability was assessed using MTS assay...
  69. Leal L, Bueno A, Gomes D, Abduch R, de Castro M, Antonini S. Inhibition of the Tcf/beta-catenin complex increases apoptosis and impairs adrenocortical tumor cell proliferation and adrenal steroidogenesis. Oncotarget. 2015;6:43016-32 pubmed publisher
    ..and non-adrenal (HeLa) cell lines were treated with PNU-74654 (5-200 μM) for 24-96 h to assess cell viability (MTS-based assay), apoptosis (Annexin V), expression/localization of beta-catenin (qPCR, immunofluorescence, ..
  70. Diaz P, Cardenas H, Orihuela P. Red Maca (Lepidium meyenii) did not affect cell viability despite increased androgen receptor and prostate-specific antigen gene expression in the human prostate cancer cell line LNCaP. Andrologia. 2016;48:922-6 pubmed publisher
    ..Red Maca aqueous extract at 0, 10, 20, 40 or 80 ?g/ml was added to LNCaP cells, and viability was evaluated by the MTS assay at 24 or 48 hr after treatment...
  71. Xie C, Gou M, Yi T, Qi X, Liu P, Wei Y, et al. Enhanced antitumor effect of biodegradable cationic heparin-polyethyleneimine nanogels delivering FILIP1L?C103 gene combined with low-dose cisplatin on ovarian cancer. Oncotarget. 2017;8:76432-76442 pubmed publisher
    ..Cisplatin (DDP) is commonly used as a first-line drug in the treatment for ovarian cancer...
  72. Huang K, Hu H, Tang Y, Xia F, Luo X, Liu J. High-Level Expression, Purification and Large-Scale Production of l-Methionine γ-Lyase from Idiomarina as a Novel Anti-Leukemic Drug. Mar Drugs. 2015;13:5492-507 pubmed publisher
    ..the enzyme significantly inhibited cellular proliferation in a concentration-dependent manner as tested using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay and induced ..
  73. Zhang N, Yang G, Shao X, Wei L. GAS5 modulated autophagy is a mechanism modulating cisplatin sensitivity in NSCLC cells. Eur Rev Med Pharmacol Sci. 2016;20:2271-7 pubmed
    In this study, we investigated the association between lncRNA GAS5 and cisplatin (DDP) resistance in NSCLC and further studied the regulative effect of GAS5 on autophagy and DDP resistance...
  74. Chen W, Chen Y, Huang Y, Hsieh B, Chiu H, Kao P, et al. Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons. Sci Rep. 2017;7:42297 pubmed publisher
    ..In addition, TPX2 localizes to the centrosome and along the neurite shaft bound to MTs. Depleting TPX2 decreases MT formation frequency specifically at the tip and the base of the neurite, and these ..
  75. Popescu R, Andronescu E, Vasile B, Trusca R, Boldeiu A, Mogoanta L, et al. Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles. Molecules. 2017;22: pubmed publisher
    ..evaluated on human BT 474 breast ductal carcinoma, HepG2 hepatocellular carcinoma and MG 63 osteosarcoma cells by MTS. In parallel, cellular morphology of these cells were examined through fluorescence microscopy and SEM...
  76. Pagliara D, Maxia S, Faenza M, Dessena L, Campus G, Rubino C. Low Versus High Vacuum Suction Drainage of the Submuscular Pocket in Primary Breast Reconstruction: A Retrospective Study. Ann Plast Surg. 2017;: pubmed publisher
    ..Days of drain permanence (DDP) are associated with hospital stay and related health care costs...
  77. Singh B, Bharadwaj P, Bajaj N, Chadha D. Endovascular management of a case of spontaneous retroperitoneal haematoma complicated with deep vein thrombosis and pulmonary embolism. BMJ Case Rep. 2017;2017: pubmed publisher
    ..SRH has been rarely reported with May-Thurner syndrome (MTS) where it occurs due to rupture of the iliac vein or venous collaterals...
  78. Liu T, Singh R, Rios Z, Bhushan A, Li M, Sheridan P, et al. Tyrosine phosphorylation of HSC70 and its interaction with RFC mediates methotrexate resistance in murine L1210 leukemia cells. Cancer Lett. 2015;357:231-41 pubmed publisher
    ..in the membrane fraction was different from the parental L1210/0 and cisplatin (CDDP)-MTX cross resistant L1210/DDP cells...
  79. Mann P, Kleinschmidt Demasters B. CNS Masson Tumors: Frequent Association With Therapeutic Radiation. Am J Surg Pathol. 2016;40:81-93 pubmed publisher
    ..b>MTs were coassociated with radiation-induced vascular malformations (1 cavernoma-like, 1 massive) only in 2 of 6 ..
  80. Chen Y, Gao D, Liu H, Lin S, Jiang Y. Drug cytotoxicity and signaling pathway analysis with three-dimensional tumor spheroids in a microwell-based microfluidic chip for drug screening. Anal Chim Acta. 2015;898:85-92 pubmed publisher
    ..was developed for mimicking physiological microenvironment of solid tumor with multicellular tumor spheroids (MTS) for anticancer drug screening...
  81. Wan X, Yuan S, Tao H, Diao L, Wang Y, Cao C, et al. The Upregulation of TRAF1 Induced by Helicobacter pylori Plays an Antiapoptotic Effect on the Infected Cells. Helicobacter. 2016;21:554-564 pubmed publisher
    ..pylori infection were detected using the standard MTS (cell viability) assay and flow cytometry, respectively. H...
  82. Sigua Rodriguez E, Goulart D, Santos Z, Alvarez Pinzon N, Olate S, de Albergaria Barbosa J. Retention and Mechanical Behavior of Attachment Systems for Implant-Retained Auricular Prostheses. J Craniofac Surg. 2017;28:134-138 pubmed publisher
    ..The mechanical cycling test was performed using the servo-hydraulic machine MTS 810-Flex Test 40 (Eden Prairie, MN) that had a 2.5?mm shift at a 10?mm/s velocity...
  83. Guo J, Wang S, Sun W, Tang Y, Jiang G, Song M. [GenomeLabTM GeXP analysis system to detect apoptosis-associated genes in A549 cells treated by recombinant human IL-24 combined with cisplatin]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:44-47 pubmed
    ..cell line A549 after treated by the recombinant human interleukin-24 (rhIL-24) combined with cisplatin (DDP)...
  84. Su B, Guan Q, Wang M, Liu N, Wei X, Wang S, et al. Calpeptin is neuroprotective against acrylamide-induced neuropathy in rats. Toxicology. 2018;400-401:1-8 pubmed publisher
    ..The calpain's overactivation causes the degrading of MAP2 and eventually leads to the destruction of microtubules (MTs), which may be one of the mechanisms of cytoskeletal damage induced by ACR.
  85. Herms A, Bosch M, Reddy B, Schieber N, Fajardo A, Rupérez C, et al. AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Nat Commun. 2015;6:7176 pubmed publisher
    ..While known to move bidirectionally along microtubules (MTs), the role of LD motion and whether it facilitates interaction with other organelles are unclear...
  86. Karanika S, Karantanos T, Kurosaka S, Wang J, Hirayama T, Yang G, et al. GLIPR1-ΔTM synergizes with docetaxel in cell death and suppresses resistance to docetaxel in prostate cancer cells. Mol Cancer. 2015;14:122 pubmed publisher
    ..synergistic effects of the docetaxel and GLIPR1-ΔTM were evaluated with DNA fragmentation, DAPI staining and MTS using paired t-test and isobologram study...
  87. Du Y, Zhu M, Zhou X, Huang Z, Zhu J, Xu J, et al. miR-20a enhances cisplatin resistance of human gastric cancer cell line by targeting NFKBIB. Tumour Biol. 2016;37:1261-9 pubmed publisher
    ..in GC plasma and tissues from patients with cisplatin-resistant gastric cancer cell line SGC7901/cisplatin (DDP)...
  88. Shamsi F. Investigation of human cell response to covalently attached RADA16-I peptide on silicon surfaces. Colloids Surf B Biointerfaces. 2016;145:470-478 pubmed publisher
    ..Scanning electron microscopy (SEM) and methyl tetrazole sulfate (MTS) assay were used to characterize the morphology and proliferation ability of human fibroblast cells on surfaces...
  89. He J, Shi J, Zhang K, Xue J, Li J, Yang J, et al. Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells. Mol Med Rep. 2017;15:1693-1701 pubmed publisher
    ..and clonogenicity, and susceptibility to cisplatin in lung adenocarcinoma A549 cells and cisplatin-resistant A549/DDP cells...
  90. Gorska Ponikowska M, Perricone U, Kuban Jankowska A, Lo Bosco G, Barone G. 2-methoxyestradiol impacts on amino acids-mediated metabolic reprogramming in osteosarcoma cells by its interaction with NMDA receptor. J Cell Physiol. 2017;232:3030-3049 pubmed publisher
    ..We used MTS assay, migration assay, flow cytometric analyses, Western blotting and immunoprecipitation techniques as well as ..
  91. M kandawire E, Mierek Adamska A, Stürzenbaum S, Choongo K, Yabe J, Mwase M, et al. Metallothionein from Wild Populations of the African Catfish Clarias gariepinus: From Sequence, Protein Expression and Metal Binding Properties to Transcriptional Biomarker of Metal Pollution. Int J Mol Sci. 2017;18: pubmed publisher
    ..i>C. gariepinus MT displays typical features of fish MTs, including 20 conserved cysteines, and seven bound divalent cations (Zn(II) or Cd(II)) when saturated...
  92. Xing R, Yang L, Jin Y, Sun L, Li C, Li Z, et al. Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis. Scand J Immunol. 2016;83:64-71 pubmed publisher
    ..The MTS assay was used to analyse RA-FLS proliferation...