TCF3

Summary

Gene Symbol: TCF3
Description: transcription factor 3
Alias: E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, NOL1-TCF3 fusion, VDR interacting repressor, class B basic helix-loop-helix protein 21, helix-loop-helix protein HE47, immunoglobulin transcription factor 1, kappa-E2-binding factor, negative vitamin D response element-binding protein, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), transcription factor 3 variant 3, transcription factor ITF-1, vitamin D receptor-interacting repressor
Species: human

Top Publications

  1. ncbi Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence
    C Murre
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 58:537-44. 1989
  2. ncbi E2A basic helix-loop-helix transcription factors in human leukemia
    David P LeBrun
    Department of Pathology, Queen s University, Kingston, Ontario, Canada
    Front Biosci 8:s206-22. 2003
  3. ncbi A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins
    C Murre
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 56:777-83. 1989
  4. pmc The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the TAL1, E47, GATA-1 and Ldb1/NLI proteins
    I A Wadman
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 16:3145-57. 1997
  5. doi E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response
    Ting Yun Liu
    Department of Pathology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan, ROC
    Mod Pathol 23:1507-17. 2010
  6. ncbi E protein silencing by the leukemogenic AML1-ETO fusion protein
    Jinsong Zhang
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 305:1286-9. 2004
  7. pmc Genomic loss of the putative tumor suppressor gene E2A in human lymphoma
    Anne Steininger
    Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany
    J Exp Med 208:1585-93. 2011
  8. pmc B-cell-specific DNA binding by an E47 homodimer
    C P Shen
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Mol Cell Biol 15:4518-24. 1995
  9. pmc E2A suppresses invasion and migration by targeting YAP in colorectal cancer cells
    Hongchao Zhao
    Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, 197 Rui Jin Er Rd, Shanghai 200025, People s Republic of China
    J Transl Med 11:317. 2013
  10. pmc Sequence of the cDNA encoding ITF-1, a positive-acting transcription factor
    P Henthorn
    Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104 6145
    Nucleic Acids Res 18:677. 1990

Detail Information

Publications230 found, 100 shown here

  1. ncbi Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence
    C Murre
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 58:537-44. 1989
    ..has recently been identified in various proteins, including two that bind to immunoglobulin enhancers (E12 and E47)...
  2. ncbi E2A basic helix-loop-helix transcription factors in human leukemia
    David P LeBrun
    Department of Pathology, Queen s University, Kingston, Ontario, Canada
    Front Biosci 8:s206-22. 2003
    The gene E2A on chromosome 19 is involved in recurrent chromosomal rearrangements associated with pediatric acute lymphoblastic leukemia...
  3. ncbi A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins
    C Murre
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 56:777-83. 1989
    ..Remarkable is the stringent conservation of hydrophobic residues present in both helices. We demonstrate that this new motif plays a crucial role in both dimerization and DNA binding...
  4. pmc The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the TAL1, E47, GATA-1 and Ldb1/NLI proteins
    I A Wadman
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 16:3145-57. 1997
    ..We now show that in erythroid cells Lmo2 forms a novel DNA-binding complex, with GATA-1, TAL1 and E2A, and the recently identified LIM-binding protein Ldb1/NLI...
  5. doi E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response
    Ting Yun Liu
    Department of Pathology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan, ROC
    Mod Pathol 23:1507-17. 2010
    ..B cells do not express markers of germinal-center B cells, such as E2A (immunoglobulin enhancer-binding factor E12/E47), B-cell chronic lymphocytic leukemia/lymphoma 6 (BCL6), or activation-induced cytidine deaminase (AID)...
  6. ncbi E protein silencing by the leukemogenic AML1-ETO fusion protein
    Jinsong Zhang
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 305:1286-9. 2004
    ....
  7. pmc Genomic loss of the putative tumor suppressor gene E2A in human lymphoma
    Anne Steininger
    Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany
    J Exp Med 208:1585-93. 2011
    The transcription factor E2A is essential for lymphocyte development. In this study, we describe a recurrent E2A gene deletion in at least 70% of patients with Sézary syndrome (SS), a subtype of T cell lymphoma...
  8. pmc B-cell-specific DNA binding by an E47 homodimer
    C P Shen
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Mol Cell Biol 15:4518-24. 1995
    B cells express a unique E-box-binding activity that contains basic helix-loop-helix (bHLH) proteins encoded by the E2A gene...
  9. pmc E2A suppresses invasion and migration by targeting YAP in colorectal cancer cells
    Hongchao Zhao
    Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, 197 Rui Jin Er Rd, Shanghai 200025, People s Republic of China
    J Transl Med 11:317. 2013
    E2A gene, which encodes two basic helix-loop-helix (bHLH) transcription factors E12 and E47, has been identified as regulator of B lymphoid hematopoiesis and suppressor of lymphoma...
  10. pmc Sequence of the cDNA encoding ITF-1, a positive-acting transcription factor
    P Henthorn
    Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104 6145
    Nucleic Acids Res 18:677. 1990
  11. ncbi Bmi-1 regulation of INK4A-ARF is a downstream requirement for transformation of hematopoietic progenitors by E2a-Pbx1
    Kevin S Smith
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Cell 12:393-400. 2003
    ..in lymphoid malignancies, but are consistently absent in pre-B cell leukemias induced by the chimeric oncoprotein E2a-Pbx1 created by t(1;19) chromosomal translocations...
  12. pmc Notch signaling induces rapid degradation of achaete-scute homolog 1
    Virote Sriuranpong
    Program in Cellular and Molecular Medicine, Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Mol Cell Biol 22:3129-39. 2002
    ..This novel function of activated Notch to rapidly degrade a class II bHLH protein may prove to be important in many contexts in development and in cancer...
  13. pmc The B lineage transcription factor E2A regulates apoptosis in chronic lymphocytic leukemia (CLL) cells
    Lela Kardava
    Department of Immunology, University of Pittsburgh School of Medicine, PA 15261, USA
    Int Immunol 23:375-84. 2011
    ..Here, we investigate the role of the major B-cell transcription factor E2A, a known regulator of B-cell survival and proliferation, to CLL persistence...
  14. ncbi Overexpression of E2A proteins induces epithelial-mesenchymal transition in human renal proximal tubular epithelial cells suggesting a potential role in renal fibrosis
    Craig Slattery
    UCD School of Biomolecular and Biomedical Sciences, UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
    FEBS Lett 580:4021-30. 2006
    ..To further examine the role of E2A proteins in EMT, E12 and E47 were overexpressed, alone and in combination, in human RPTEC...
  15. doi Mapping acetylation sites in E2A identifies a conserved lysine residue in activation domain 1 that promotes CBP/p300 recruitment and transcriptional activation
    Brandy D Hyndman
    Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario, Canada
    Biochim Biophys Acta 1819:375-81. 2012
    E-proteins are basic helix-loop-helix transcription factors that function in cell type specification. The gene E2A encodes two E-proteins, E12 and E47, which are required in B-lymphopoiesis...
  16. doi Control of breast cancer growth and initiation by the stem cell-associated transcription factor TCF3
    Michal Slyper
    Authors Affiliations Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel Canada, Hadassah School of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel
    Cancer Res 72:5613-24. 2012
    ..b>TCF3 (also known as TCF7L1) is a member of the TCF/LEF transcription factor family that is central in regulating ..
  17. ncbi Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo
    A B Lassar
    Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Cell 66:305-15. 1991
    ..Third, association with E47 alters the phosphorylation state of MyoD. Fourth, C3H10T1/2 cells expressing antisense E2A transcripts contain low levels of E2A gene products and display less terminal muscle differentiation when infected ..
  18. pmc Notch-induced E2A ubiquitination and degradation are controlled by MAP kinase activities
    Lei Nie
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    EMBO J 22:5780-92. 2003
    ..Here, we report that signaling through Notch modulates the turnover of E2A proteins including E12 and E47, which are basic helix-loop-helix proteins crucial for B and T lymphocyte ..
  19. pmc Gene expression patterns define novel roles for E47 in cell cycle progression, cytokine-mediated signaling, and T lineage development
    Ruth Schwartz
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0377, USA
    Proc Natl Acad Sci U S A 103:9976-81. 2006
    In maturing T lineage cells, the helix-loop-helix protein E47 has been shown to enforce a critical proliferation and developmental checkpoint commonly referred to as beta selection...
  20. doi Tcf3 and cell cycle factors contribute to butyrate resistance in colorectal cancer cells
    Christopher Chiaro
    Department of Basic Sciences, The Commonwealth Medical College, 525 Pine Street, Scranton, PA 18509, USA
    Biochem Biophys Res Commun 428:121-6. 2012
    ..We identified overexpression of Tcf3 as being partially responsible for the butyrate-resistant phenotype, as this DNA-binding protein suppresses the ..
  21. pmc Increased expression of bHLH transcription factor E2A (TCF3) in prostate cancer promotes proliferation and confers resistance to doxorubicin induced apoptosis
    Divya Patel
    Center for Cancer Research and Therapeutics Development, Clark Atlanta University, 223 James P Brawley DR SW, Atlanta, GA 30314, USA
    Biochem Biophys Res Commun 422:146-51. 2012
    E2A (TCF3) is a multifunctional basic helix loop helix (bHLH), transcription factor. E2A regulates transcription of target genes by homo- or heterodimerization with cell specific bHLH proteins...
  22. doi miR-495 is upregulated by E12/E47 in breast cancer stem cells, and promotes oncogenesis and hypoxia resistance via downregulation of E-cadherin and REDD1
    W W Hwang-Verslues
    Genomics Research Center, Academia Sinica, Taipei, Taiwan
    Oncogene 30:2463-74. 2011
    ..miR-495 expression was directly modulated by transcription factor E12/E47, which itself is highly expressed in BCSCs...
  23. ncbi E12 and E47 modulate cellular localization and proteasome-mediated degradation of MyoD and Id1
    Jody M Lingbeck
    Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Oncogene 24:6376-84. 2005
    ..E12 and E47, splice products of the E2A gene, interact with MyoD to activate transcription of the muscle program and are also degraded by the ubiquitin-..
  24. pmc Selective use of multiple vitamin D response elements underlies the 1 alpha,25-dihydroxyvitamin D3-mediated negative regulation of the human CYP27B1 gene
    Mikko M Turunen
    Department of Biochemistry, University of Kuopio, FIN 70211 Kuopio, Finland
    Nucleic Acids Res 35:2734-47. 2007
    ..This data explains, in part, why the human CYP27B1 gene is repressed in HEK-293 but not in MCF-7 cells...
  25. ncbi The effects of siRNA-mediated inhibition of E2A-PBX1 on EB-1 and Wnt16b expression in the 697 pre-B leukemia cell line
    Giovanna Casagrande
    Laboratory of Hematology and Oncology, Department of Pediatrics, University of Padova, Italy
    Haematologica 91:765-71. 2006
    ..acute lymphoblastic leukemia (ALL) is t(1;19)(q23;p13), usually resulting in the expression of the chimeric gene E2A-PBX1...
  26. pmc Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function
    S H Ou
    Department of Medicine, University of Texas Southwestern Medical Center at Dallas 75235 8594
    J Virol 68:7188-99. 1994
    ..Two proteins representing different classes of bHLH proteins, E47 and AP-4, were assayed for their ability to bind to the flanking E box motifs...
  27. ncbi The gene for enhancer binding proteins E12/E47 lies at the t(1;19) breakpoint in acute leukemias
    J D Mellentin
    Department of Pathology, Stanford University School of Medicine, CA 94025
    Science 246:379-82. 1989
    The gene (E2A) that codes for proteins with the properties of immunoglobulin enhancer binding factors E12/E47 was mapped to chromosome region 19p13.2-p13.3, a site associated with nonrandom translocations in acute lymphoblastic leukemias...
  28. pmc Gene deregulation and spatial genome reorganization near breakpoints prior to formation of translocations in anaplastic large cell lymphoma
    Stephan Mathas
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Proc Natl Acad Sci U S A 106:5831-6. 2009
    ..Also, our data demonstrate that deregulation of breakpoint-proximal genes has a key role in ALCL...
  29. pmc Bridge-1, a novel PDZ-domain coactivator of E2A-mediated regulation of insulin gene transcription
    M K Thomas
    Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Harvard Medical School, and Howard Hughes Medical Institute, Boston, Massachusetts 02114, USA
    Mol Cell Biol 19:8492-504. 1999
    Proteins in the E2A family of basic helix-loop-helix transcription factors are important in a wide spectrum of physiologic processes as diverse as neurogenesis, myogenesis, lymphopoeisis, and sex determination...
  30. pmc Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching
    Akiko Murayama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo, Japan
    EMBO J 23:1598-608. 2004
    ..A bHLH-type factor, designated VDIR, was identified as a direct sequence-specific activator of 1nVDRE...
  31. ncbi Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription
    Noriko Funato
    Human Gene Sciences Center and Maxillofacial Orthognathics Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8510, Japan
    J Biol Chem 278:7486-93. 2003
    ..The yeast and mammalian two-hybrid systems showed physical associations between epicardin/capsulin/Pod-1 and E2A, both of which were present in the nuclei. The bHLH domains mediated this association...
  32. ncbi E47, IRF-4, and PU.1 synergize to induce B-cell-specific activation of the class II transactivator promoter III (CIITA-PIII)
    Nienke van der Stoep
    Division of Molecular Biology, Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, E3 Q, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 104:2849-57. 2004
    ..We show that in B cells, E47 and PU...
  33. ncbi HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex
    P Persson
    Department of Laboratory Medicine, Lund University, Malmo, University Hospital MAS, S 205 02, Sweden
    Biochem Biophys Res Commun 274:22-31. 2000
    ..In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems...
  34. pmc A pentamer transcriptional complex including tal-1 and retinoblastoma protein downmodulates c-kit expression in normal erythroblasts
    L Vitelli
    Department of Hematology Oncology, Istituto Superiore di Sanita, 00161 Rome, Italy
    Mol Cell Biol 20:5330-42. 2000
    ..contain a pentamer protein complex comprising the tal-1 transcription factor heterodimerized with the ubiquitous E2A protein and linked to Lmo2, Ldb1, and retinoblastoma protein (pRb)...
  35. pmc Characterization of ABF-1, a novel basic helix-loop-helix transcription factor expressed in activated B lymphocytes
    M E Massari
    Department of Biology, University of California, San Diego, La Jolla, California 92093, USA
    Mol Cell Biol 18:3130-9. 1998
    ..ABF-1 contains a transcriptional repression domain and is capable of inhibiting the transactivation capability of E47 in mammalian cells...
  36. pmc Notch1 promotes survival of E2A-deficient T cell lymphomas through pre-T cell receptor-dependent and -independent mechanisms
    Erica J Reschly
    Department of Pathology, MC1089, The University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
    Blood 107:4115-21. 2006
    Loss of E2A transcription factor activity or activation of the intracellular form of Notch1 (ICN) leads to the development of leukemia or lymphoma in humans or mice, respectively...
  37. ncbi A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL
    M P Kamps
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142
    Cell 60:547-55. 1990
    ..3) translocation, found in human pre-B cell acute lymphoblastic leukemias, is within the E2A transcription factor gene on chromosome 19...
  38. ncbi The t(1;19)(q23;p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias
    S P Hunger
    Department of Pathology, Stanford University School of Medicine, CA
    Blood 77:687-93. 1991
    ..The t(1;19) juxtaposes the E2A gene from chromosome 19 with the PBX1 gene on chromosome 1, leading to the production of fusion transcripts and ..
  39. ncbi Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor
    J Nourse
    Department of Pathology, Stanford University School of Medicine, California 94305
    Cell 60:535-45. 1990
    The gene (E2A) for enhancer binding transcription factors E12 and E47 maps to the t(1;19) chromosomal translocation breakpoint in pre-B cell leukemias...
  40. ncbi E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements
    G Bain
    Department of Biology, University of California, San Diego, La Jolla 92130
    Cell 79:885-92. 1994
    E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression...
  41. ncbi Characterization of the mUBC9-binding sites required for E2A protein degradation
    G S Huggins
    Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 274:28690-6. 1999
    Mammalian Ubc9 (mUbc9) is required for rapid degradation of the E2A proteins E12 and E47 by the ubiquitin-proteasome system. We have shown elsewhere that mUbc9 interacts with amino acids 477-530 of E12/E47...
  42. pmc Synergistic activation of the human orphan nuclear receptor SHP gene promoter by basic helix-loop-helix protein E2A and orphan nuclear receptor SF-1
    Han Jong Kim
    Hormone Research Center, Chonnam National University, Kwangju, 500 757, Republic of Korea
    Nucleic Acids Res 31:6860-72. 2003
    ..In this study, we have found that basic helix-loop-helix (bHLH) transcription factors, the E2A proteins (E47, E12 and E2/5), activated the human but not the mouse SHP promoter...
  43. pmc Association of connexin36 and zonula occludens-1 with zonula occludens-2 and the transcription factor zonula occludens-1-associated nucleic acid-binding protein at neuronal gap junctions in rodent retina
    C Ciolofan
    Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 3J7
    Neuroscience 140:433-51. 2006
    ....
  44. pmc Genes on bovine chromosome 18 associated with bilateral convergent strabismus with exophthalmos in German Brown cattle
    S Fink
    Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
    Mol Vis 14:1737-51. 2008
    ..association analysis for three candidate genes (troponin T type 1 [TNNT1], retinol dehydrogenase 13 [RDH13], and TCF3 fusion partner [TFPT]), which are located within the previously identified BCSE-linked region on the telomeric end ..
  45. ncbi Transcriptional regulation of activating transcription factor 3 involves the early growth response-1 gene
    Frank G Bottone
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 315:668-77. 2005
    ..Therefore, this is a novel first report demonstrating that the expression of ATF3 occurs via Egr-1 downstream of Erk1/2...
  46. pmc Activating transcription factor 3 is integral to the eukaryotic initiation factor 2 kinase stress response
    Hao Yuan Jiang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Cell Biol 24:1365-77. 2004
    ....
  47. ncbi Development of packaging cell lines for generation of adeno-associated virus vectors by lentiviral gene transfer of trans-complementary components
    Satoki Nakamura
    Department of Internal Medicine III, Hamamatsu University School of Medicine, Handayama, Japan
    Eur J Haematol 73:285-94. 2004
    ..va.e2a.e4...
  48. ncbi Characterization of adeno-associated virus rep protein inhibition of adenovirus E2a gene expression
    Shadia Nada
    Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614 5804, USA
    Virology 293:345-55. 2002
    ..In this report we investigate Rep-mediated effects on expression of the adenovirus (Ad) E2a gene and the Ad major late promoter...
  49. ncbi Sequence-specific methylation in a downstream region of the late E2A promoter of adenovirus type 2 DNA prevents protein binding
    R Hermann
    Institute of Genetics, University of Cologne, F R G
    J Mol Biol 210:411-5. 1989
    In studies on the promoter-inhibitory effect of sequence-specific DNA methylations, the late E2A promoter of human adenovirus type 2 (Ad2) has been used as an experimental tool...
  50. ncbi Activating transcription factor 3 induction in sympathetic neurons after axotomy: response to decreased neurotrophin availability
    H Hyatt Sachs
    Department of Neurosciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4975, USA
    Neuroscience 150:887-97. 2007
    ....
  51. doi Expression of RUNX3 gene, methylation status and clinicopathological significance in breast cancer and breast cancer cell lines
    Ying Jiang
    Department of Pathology, Second Affiliated Hospital of Harbin Medical University, Harbin, China
    Pathobiology 75:244-51. 2008
    ..In this study, we aimed to analyze the methylation status of the RUNX3 promoter in breast cancer and to evaluate the relationship between RUNX3 expression and breast carcinogenesis and prognosis...
  52. ncbi Axotomy-induced dopaminergic neurodegeneration is accompanied with c-Jun phosphorylation and activation transcription factor 3 expression
    Dae Y Song
    Department of Anatomy and Neuroscience, Eulji University School of Medicine, Daejeon, Republic of Korea
    Exp Neurol 209:268-78. 2008
    ..Taken together, these results support the idea that pc-Jun and its putative dimeric partner ATF3 may be closely participating in axotomy-induced neurodegeneration...
  53. pmc High incidence of proviral integrations in the Hoxa locus in a new model of E2a-PBX1-induced B-cell leukemia
    Janet Bijl
    Laboratory of Molecular Genetics of Stem Cells, Institute for Research in Immunology and Cancer, Montreal, Quebec H3C 3J7, Canada
    Genes Dev 19:224-33. 2005
    Relevant mouse models of E2a-PBX1-induced pre-B cell leukemia are still elusive...
  54. pmc Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes
    S Ikushima
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 94:2609-14. 1997
    The E2A-HLF (hepatic leukemia factor) oncoprotein, generated in pro-B lymphocytes by fusion of the trans-activation domain of E2A to the basic region/leucine zipper (bZIP) domain of HLF, functions as an anti-apoptotic transcription ..
  55. pmc HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors
    J S Hu
    Department of Genetics, Harvard Medical School, Boston, Massachusetts
    Mol Cell Biol 12:1031-42. 1992
    ..nearly complete identity in the B-HLH domain with the immunoglobulin enhancer binding proteins encoded by the E2A and ITF2 genes (E proteins)...
  56. pmc Pan/E2A expression precedes immunoglobulin heavy-chain expression during B lymphopoiesis in nontransformed cells, and Pan/E2A proteins are not detected in myeloid cells
    Y Jacobs
    Department of Biochemistry, University of California, Riverside 92521
    Mol Cell Biol 14:4087-96. 1994
    A newly developed rat long-term bone marrow culture system was used to study the role of Pan/E2A basic helix-loop-helix transcription factors during B-cell development...
  57. ncbi Levels of E2A protein expression in B cell precursors are stage-dependent and inhibited by stem cell factor (c-kit ligand)
    Richard L Riley
    Department of Microbiology and Immunology, University of Miami School of Medicine, PO Box 016960 R138, Miami, FL 33101, USA
    Exp Hematol 30:1412-8. 2002
    The E2A-encoded proteins E47 and E12 are crucial to the development of pro-B and pre-B cells...
  58. pmc Early B cell factor promotes B lymphopoiesis with reduced interleukin 7 responsiveness in the absence of E2A
    Christopher S Seet
    Department of Pathology, University of Chicago, 5841 S Maryland Avenue, MC 1089, Chicago, IL 60637, USA
    J Exp Med 199:1689-700. 2004
    The basic helix-loop-helix transcription factors encoded by the E2A gene function at the apex of a transcriptional hierarchy involving E2A, early B cell factor (EBF), and Pax5, which is essential for B lymphopoiesis...
  59. pmc Effects of adeno-associated virus on adenovirus replication and gene expression during coinfection
    Jennifer M Timpe
    Department of Biochemistry and Cancer Biology, Medical University of Ohio, 3035 Arlington Ave, Toledo, OH 43614 5804, USA
    J Virol 80:7807-15. 2006
    ..E4 and late transcription were the most strongly inhibited, and E1A and E2A were the least affected. The temporal effects of AAV on Ad mRNA transcript levels also varied among the Ad genes...
  60. ncbi Inhibition of adenovirus cytotoxicity, replication, and E2a gene expression by adeno-associated virus
    X J Jing
    Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614 5804, USA
    Virology 291:140-51. 2001
    ..AAV, but not UV-inactivated AAV, delayed Ad-induced cytotoxicity and inhibited Ad E2a gene expression. AAV, but not UV-inactivated AAV or a recombinant AAV vector, inhibited Ad DNA replication...
  61. ncbi Activating transcription factor 3 is up-regulated in patients with hypospadias
    Benchun Liu
    Center for the Study and Treatment of Hypospadias, Department of Urology, University of California, San Francisco 94143, USA
    Pediatr Res 58:1280-3. 2005
    ..Because the etiology of hypospadias may include exposure to estrogenic compounds, the responsiveness of ATF3 to estrogen is also discussed...
  62. doi [Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage]
    Wen Sun
    Department of Traditional Chinese Medicine, Children s Hospital, Fudan University, Shanghai 201102, China
    Zhong Xi Yi Jie He Xue Bao 8:341-6. 2010
    ..To explore the effects of Cordyceps extract in regulating the imbalance of Th1/Th2 ratio and inhibiting the inflammatory reaction, and to find the theoretical basis of Cordyceps extract for treating asthma in remission stage...
  63. ncbi Prolonged transgene expression in cotton rat lung with recombinant adenoviruses defective in E2a
    J F Engelhardt
    Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia 19104 4268
    Hum Gene Ther 5:1217-29. 1994
    ..that is disabled in its capacity to replicate by the introduction of a temperature-sensitive mutation in the E2a gene as well deletion of E1 sequences...
  64. doi Activation transcription factor-3 activation and the development of spinal cord degeneration in a rat model of amyotrophic lateral sclerosis
    A Malaspina
    Centre for Neuroscience and Trauma, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Blizard Institute, 4 Newark Street, London E1 2AT, UK
    Neuroscience 169:812-27. 2010
    ..In addition, factors previously described to be linked to ATF-3 activation under various experimental conditions of stress, become switched on in spinal cord from the end-stage transgenic rat model of ALS...
  65. pmc Cloning and functional characterisation of avian transcription factor E2A
    Thomas M Conlon
    Cambridge Institute for Medical Research and Department of Pathology, University of Cambridge, WT MRC building, Addenbrooke s Hospital, CB2 2XY, UK
    BMC Immunol 5:11. 2004
    During B lymphocyte development the E2A gene is a critical regulator of cell proliferation and differentiation...
  66. pmc Temporal regulation of Ig gene diversification revealed by single-cell imaging
    Ellen C Ordinario
    Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Immunol 183:4545-53. 2009
    ..We recently showed that the activator, E2A, functions in cis to promote diversification, and that G(1) phase of cell cycle is the critical window for E2A ..
  67. pmc Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1
    P S Knoepfler
    Department of Pathology, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 94:14553-8. 1997
    b>E2a-Pbx1 is a chimeric transcription factor oncoprotein produced by the t(1;19) translocation in human pre-B cell leukemia...
  68. pmc The embryonal carcinoma stem cell Ela-like activity involves a differentiation-regulated transcription factor
    N B La Thangue
    Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Nucleic Acids Res 18:2929-38. 1990
    ..The Ela-inducible adenovirus E2A promoter has been used to further define this activity and we show that in vitro the transcription of this promoter ..
  69. pmc Simian virus 40 small t antigen trans activates the adenovirus E2A promoter by using mechanisms distinct from those used by adenovirus E1A
    M R Loeken
    Joslin Diabetes Center, Boston, Massachusetts 02215
    J Virol 66:2551-5. 1992
    ..simian virus 40 small t antigen, polyomavirus small t antigen stimulates transcription directed by the adenovirus E2A and VA-I promoters during transient transfection assays...
  70. pmc c-jun inhibits insulin control element-mediated transcription by affecting the transactivation potential of the E2A gene products
    G L Robinson
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee 37232
    Mol Cell Biol 15:1398-404. 1995
    ..The ICE activator is composed, in part, of the basic helix-loop-helix proteins E12, E47, and E2-5 encoded by the E2A gene...
  71. pmc E2A-HLF-mediated cell transformation requires both the trans-activation domains of E2A and the leucine zipper dimerization domain of HLF
    T Yoshihara
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Mol Cell Biol 15:3247-55. 1995
    The E2A-HLF fusion gene, formed by the t(17;19)(q22;p13) translocation in childhood acute pro-B-cell leukemia, encodes a hybrid protein that contains the paired trans-activation domains of E2A (E12/E47) linked to the basic region/leucine ..
  72. ncbi Identification of a candidate regulatory element within the 5' flanking region of the mouse Igh locus defined by pro-B cell-specific hypersensitivity associated with binding of PU.1, Pax5, and E2A
    Inka Pawlitzky
    Immunology Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    J Immunol 176:6839-51. 2006
    ..Sequence motifs within HS1 for PU.1, Pax5, and E2A bind these proteins in vitro and these factors are recruited to HS1 sequence only in pro-B cells...
  73. pmc DNA-binding and transcriptional regulatory properties of hepatic leukemia factor (HLF) and the t(17;19) acute lymphoblastic leukemia chimera E2A-HLF
    S P Hunger
    Department of Pathology, Stanford University School of Medicine, California 94305
    Mol Cell Biol 14:5986-96. 1994
    The t(17;19) translocation in acute lymphoblastic leukemias results in creation of E2A-hepatic leukemia factor (HLF) chimeric proteins that contain the DNA-binding and protein dimerization domains of the basic leucine zipper (bZIP) ..
  74. ncbi Immunologic regulation of bone development
    Mark C Horowitz
    Yale University School of Medicine, Department of Orthopaedics and Rehabilitation, New Haven, CT, USA
    Adv Exp Med Biol 602:47-56. 2007
    A regulatory network comprised of transcription factors PU.1, Ikaros, E2A, EBF, and Pax5 control B cell fate specification and differentiation...
  75. ncbi Formation of der(19)t(1;19)(q23;p13) in acute lymphoblastic leukemia
    Kajsa Paulsson
    Department of Clinical Genetics, Lund University Hospital, Lund, Sweden
    Genes Chromosomes Cancer 42:144-8. 2005
    The t(1;19)(q23;p13), which results in a fusion of TCF3 (previously E2A) at 19p13 with PBX1 at 1q23, is one of the most common translocations in acute lymphoblastic leukemia (ALL)...
  76. doi In vivo interactions of MyoD, Id1, and E2A proteins determined by acceptor photobleaching fluorescence resonance energy transfer
    Jody M Lingbeck
    Edward Mallinckrodt Department of Pediatrics and Molecular Biology and Pharmacology, Washington University School of Medicine and St Louis Children s Hospital, St Louis, Missouri 63110, USA
    FASEB J 22:1694-701. 2008
    ..Furthermore, MyoD has been shown to modulate the ubiquitin-mediated degradation of Id1 and E2A proteins, E12 and E47. The molecular mechanisms governing these events are not clear but are hypothesized to occur via heterodimer ..
  77. pmc Increased risk for CNS relapse in pre-B cell leukemia with the t(1;19)/TCF3-PBX1
    S Jeha
    Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Leukemia 23:1406-9. 2009
    ..therapy on the clinical outcome of children with pre-B acute lymphoblastic leukemia (ALL) and the t(1;19)/TCF3/PBX1, we analyzed 735 patients with B-cell precursor ALL treated in four successive protocols at St Jude Children's ..
  78. doi Plasmids with E2 epitope tags: tagging modules for N- and C-terminal PCR-based gene targeting in both budding and fission yeast, and inducible expression vectors for fission yeast
    Tiina Tamm
    Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu 51010, Estonia
    Yeast 26:55-66. 2009
    ..E2Tags are 10-amino acids (epitope E2a: SSTSSDFRDR)- and 12 amino acids (epitope E2b: GVSSTSSDFRDR)-long peptides derived from the E2 protein of bovine ..
  79. ncbi Expression of activating transcription factor 3 and growth-associated protein 43 in the rat geniculate ganglion neurons after chorda tympani injury
    Kenzo Tsuzuki
    Department of Otorhinolaryngology, Hyogo College of Medicine, Japan
    Acta Otolaryngol 122:161-7. 2002
    ....
  80. doi Multiple T cell epitope peptides suppress allergic responses in an egg allergy mouse model by the elicitation of forkhead box transcription factor 3- and transforming growth factor-beta-associated mechanisms
    M Yang
    Department of Food Science, University of Guelph, ON, Canada
    Clin Exp Allergy 40:668-78. 2010
    ..Peptide-based immunotherapy (PIT) represents an attractive approach for targeted interventions in immunological disorders, but has not been widely explored in the context of food allergy...
  81. ncbi Interleukin-10 induced activating transcription factor 3 transcriptional suppression of matrix metalloproteinase-2 gene expression in human prostate CPTX-1532 Cells
    Mark E Stearns
    Department of Pathology, College of Medicine, Drexel University, MS 435, 15th and Vine Streets, Philadelphia, PA 19085, USA
    Mol Cancer Res 2:403-16. 2004
    ..Taken together, these data demonstrate the critical role of tyrosine phosphorylated ATF3 and the CREB consensus domain in IL-10 suppression of MMP-2 gene expression in primary human prostate tumor cells...
  82. pmc Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation
    Q Lu
    Department of Chemistry, University of California, San Diego, La Jolla 92093
    Mol Cell Biol 14:3938-48. 1994
    b>E2A-PBX1 is a chimeric gene formed by the t(1;19)(q23;p13.3) chromosomal translocation of pediatric pre-B-cell leukemia...
  83. pmc Early B-cell factor, E2A, and Pax-5 cooperate to activate the early B cell-specific mb-1 promoter
    Mikael Sigvardsson
    Department for Stem Cell Biology, Lund, Sweden
    Mol Cell Biol 22:8539-51. 2002
    ..In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells...
  84. ncbi Reactivation of the methylation-inactivated late E2A promoter of adenovirus type 2 by E1A (13 S) functions
    B Weisshaar
    Institute of Genetics, University of Cologne, West Germany
    J Mol Biol 202:255-70. 1988
    The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA...
  85. pmc The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex
    S Obert
    Department of Microbiology, State University of New York, Stony Brook 11794
    Mol Cell Biol 14:1333-46. 1994
    Binding of the mammalian transcription factor E2F to the adenovirus E2a early promoter is modulated through interaction with the viral E4-6/7 protein...
  86. pmc Clinical features and prognostic implications of TCF3-PBX1 and ETV6-RUNX1 in adult acute lymphoblastic leukemia
    Thomas Burmeister
    Med Klinik für Hämatologie Onkologie Hindenburgdamm 30, 12200 Berlin, Germany
    Haematologica 95:241-6. 2010
    ..However, the prognostic impact of other rarer fusion genes is less well established in adult acute lymphoblastic leukemia than in the childhood form...
  87. ncbi [Correlation of E2a-pbx1 expression level with clinical characteristics and early response to treatment in children with acute lymphoblastic leukemia]
    Chao Gao
    Center of Hematology, Beijing Children Hospital, Capital University of Medical Sciences, Beijing 100045, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 16:569-73. 2008
    This study purposed to investigate the correlation of expression level of e2a-pbx1 (immunoglobulin enhancer binding factor-Pre-B leukemia) with clinical characteristics and early response to treatment in children patients with acute ..
  88. pmc E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts
    X Fu
    Department of Pathology, University of California, San Diego, School of Medicine, La Jolla 92093, USA
    Mol Cell Biol 17:1503-12. 1997
    The E2a-Pbx1 oncoprotein contains the transactivation domain of E2a joined to the DNA-binding homeodomain (HD) of Pbx1. In mice, E2a-Pbx1 transforms T lymphoblasts and fibroblasts and blocks myeloblast differentiation...
  89. ncbi The chimeric oncoproteins E2A-PBX1 and E2A-HLF are concentrated within spherical nuclear domains
    D P LeBrun
    Department of Pathology, Queen s University, Kingston, Ontario, Canada
    Oncogene 15:2059-67. 1997
    ..The leukemogenic transcription factor gene E2A-PBX1 is created through fusion of the genes E2A and PBX1 as a result of t(1;19) in acute lymphoblastic leukemia...
  90. doi Activating transcription factor 3: a hormone responsive gene in the etiology of hypospadias
    Ana Beleza-Meireles
    Department of Molecular Medicine and Surgery, Karolinska Institutet, SE 171 76 Stockholm, Sweden
    Eur J Endocrinol 158:729-39. 2008
    ..Activating transcription factor 3 (ATF3), an estrogen responsive gene, has been reported to be expressed during sexual development and up-regulated in hypospadic genital skin. We investigated ATF3 as a candidate gene for hypospadias...
  91. ncbi Specific factors binding to the late E2A promoter region of adenovirus type 2 DNA: no apparent effects of 5'-CCGG-3' methylation
    A Hoeveler
    Institute of Genetics, University of Cologne, Germany
    DNA 6:449-60. 1987
    ..g., by the E1 proteins of adenoviruses. We studied the inactivation of the late E2A promoter of adenovirus type 2 (Ad2) DNA by the methylation of three 5'-CCGG-3' sequences at positions -215, +5, and ..
  92. pmc Role of activating transcription factor 3 on TAp73 stability and apoptosis in paclitaxel-treated cervical cancer cells
    Yeo Kyoung Oh
    Center for Biomedical Sciences, NIH, Seoul 122 701, Korea
    Mol Cancer Res 6:1232-49. 2008
    ..The investigation shows that ATF3 may function as a tumor-inhibiting factor through direct regulatory effects on TAp73beta, suggesting a functional link between ATF3 and TAp73beta...
  93. pmc Identification of E2A target genes in B lymphocyte development by using a gene tagging-based chromatin immunoprecipitation system
    Stephen Greenbaum
    Department of Immunology, Duke University Medical Center, Box 3010, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 99:15030-5. 2002
    The transcription factors encoded by the E2A gene are known to be essential for B lymphocyte development, and ectopic expression or gene inactivation studies have revealed several potential lineage-specific E2A target genes...
  94. ncbi Establishment of de novo DNA methylation patterns. Transcription factor binding and deoxycytidine methylation at CpG and non-CpG sequences in an integrated adenovirus promoter
    M Toth
    Institute for Genetics, University of Cologne, West Germany
    J Mol Biol 214:673-83. 1990
    ..to occur across an entire integrated adenovirus genome as well as at the nucleotide level in the integrated late E2A promoter of adenovirus type 2...
  95. ncbi [A clinical and laboratory study of TCF3-PBX1 positive adult acute lymphoblastic leukemia.]
    Ji Fu Zheng
    Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Thrombosis and Hemostasis Key Lab of the Ministry of Health, Suzhou 215006, China
    Zhonghua Xue Ye Xue Za Zhi 31:16-20. 2010
    To explore the morphology, immunophenotype, cytogenetics and clinical features of TCF3-PBX1 fusion gene positive adult acute lymphoblastic leukemia (ALL).
  96. ncbi Estradiol upregulates activating transcription factor 3, a candidate gene in the etiology of hypospadias
    Benchun Liu
    Department of Urology, University of California, San Francisco, CA, USA
    Pediatr Dev Pathol 10:446-54. 2007
    ..Its potential involvement in other manifestations of developmental endocrine disruption is worth investigating...
  97. ncbi Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation
    R Dasgupta
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology and Committee on Developmental Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 126:4557-68. 1999
    ..TOPGAL expression was directly stimulated by a stabilized form of beta -catenin, but was also dependent upon LEF1/TCF3 in skin...
  98. ncbi Transfer of the CFTR gene to the lung of nonhuman primates with E1-deleted, E2a-defective recombinant adenoviruses: a preclinical toxicology study
    M J Goldman
    Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia, USA
    Hum Gene Ther 6:839-51. 1995
    ..This second-generation virus is deleted of E1 and contains a temperature-sensitive mutation in the E2a gene, which encodes a defective DNA-binding protein. Two distinct projects were undertaken...
  99. doi Lineage-instructive function of C/EBPα in multipotent hematopoietic cells and early thymic progenitors
    Albert Wölfler
    Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands
    Blood 116:4116-25. 2010
    ..C/EBPα induced a strong myeloid gene expression signature and down-regulated E2A-induced regulators of early lymphoid development...
  100. pmc Inhibition of cortical neuron differentiation by Groucho/TLE1 requires interaction with WRPW, but not Eh1, repressor peptides
    Manuel Buscarlet
    Centre for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
    J Biol Chem 283:24881-8. 2008
    ..the L743F mutation does not disrupt binding to Engrailed1 and FoxG1, which both contain Eh1 motifs, nor to Tcf3, which binds to the Gro/TLE N terminus...
  101. doi Identification of new Nerve Growth Factor-responsive immediate-early genes
    T F Dijkmans
    Division of Medical Pharmacology, Leiden Amsterdam Center for Drug Research, Leiden University Medical Center, PO Box 9502, 2300 RA, Leiden, The Netherlands
    Brain Res 1249:19-33. 2009
    ..These observations may therefore provide important new information on the molecular mechanisms underlying NGF-induced differentiation...

Research Grants64

  1. Myeloid Lineage Differentiation and Osteoclast Priming by a Novel Pax5 Cytokine
    Mark C Horowitz; Fiscal Year: 2013
    ..1, Ikaros, E2A, Ebf1, Pax5 and Gon4-like. With the exception of PU...
  2. REGULATION OF EPIDERMAL DEVELOPMENT AND DIFFERENTIATION
    Elaine Fuchs; Fiscal Year: 2013
    ..2-Catenin acts as a co-factor for the Lef1/Tcf family of DNA binding proteins. In HF-SCs, two family members-- Tcf3 and Tcf4--are co-expressed, where they function redundantly to repress differentiation in the absence of Wnt ..
  3. ALTERED SENSIBILITY FOLLOWING PERIPHERAL NERVE DAMAGE
    Clifford J Woolf; Fiscal Year: 2013
    ....
  4. Notch-induced protein degradation in lymphopoiesis
    Xiao Hong Sun; Fiscal Year: 2013
    ..e. Notch ligation results in degradation of the E2A transcription factor...
  5. Transcriptional regulatory mechanisms in B cell development and leukemogenesis
    Robert G Roeder; Fiscal Year: 2013
    ..The transcription factor E2A plays a central role in early B cell development through synergistic functions with other lineage-restricted ..
  6. GATA3 deregulation and T cell transformation in E2A-/- mice
    BARBARA LYNNE KEE; Fiscal Year: 2012
    ..The E2A transcription factors are also required for normal T cell differentiation and deletion of E2A, or inhibition of E2A ..
  7. Atherogenic mechanism of lipid peroxidation-derived aldehydes
    Sanjay Srivastava; Fiscal Year: 2013
    ..Results obtained from this project will help in developing a better understanding, and potentially, novel therapeutic strategies for decreasing or managing atherosclerosis. ..
  8. Regulation of Immunoglobulin Class Switch in Aged Mice
    Bonnie B Blomberg; Fiscal Year: 2013
    ..preliminary data to establish the intrinsic splenic B cell defect in Ig class switch in aged BALB/c, C57BL/6 and E2A+/- mice...
  9. Mechanisms of flagellin-induced protection against microbial keratitis
    Fu Shin X Yu; Fiscal Year: 2013
    ..abstract_text> ..
  10. Skin Stem Cells: Purification and Characterization
    Elaine Fuchs; Fiscal Year: 2013
    ..We showed that HF-SCs express a group of transcription factors-- Sox9, Lhx2, NFATc1 &Tcf3/4--which basal IFE SCs do not, and we used these markers to trace the origins of HF-SCs to embryogenesis...
  11. Molecular Events Initiating B Cell Fate Determination
    KAY LYNN MEDINA; Fiscal Year: 2013
    ..Flt3 signaling, in a ligand-dose dependent fashion, regulates the activity of the primary B cell fate determinant, E2A, a key regulator of the EBF gene. Once expressed, EBF orchestrates B cell differentiation...
  12. ATF3-Mediated Transcriptional Repression of Interferon-beta
    STEVE LEE WEINSTEIN; Fiscal Year: 2010
    ..The possibility that repression of IFN-? transcription by ATF-3 is caused by histone deacetylation will be tested by examining the acetylation state of IFN-? promoter histones in the presence or absence of ATF-3 by ChlP. ..
  13. Targeting Leukemia causing oncogene E2a-Pbx1 with synthetic molecules
    Aseem Z Ansari; Fiscal Year: 2012
    ..A major goal of this proposal is to define the DNA regulatory sites of two oncogenic transcription factors, E2a- Pbx1 and HoxA9...
  14. MECHANISMS OF LYMPHOID LINEAGE COMMITMENT
    David M Allman; Fiscal Year: 2013
    ..which state that B-lineage restriction is directly regulated by two alternative transcription factors known as E2a and Pax5...
  15. 14-3-3?? and epithelial differentiation in the eye and other tissues
    Qiutang Li; Fiscal Year: 2012
    ..Oscillation of the transcription factor TCF3 between activator and repressor is key to both maintaining a resident stem cell population and generating pulses of ..
  16. BIOCHEMISTRY AND REGULATION OF V(D)J RECOMBINATION
    Mark S Schlissel; Fiscal Year: 2012
    ..and RSS end insertion during V(D)J recombination in vivo;4) examine in detail how a critical transcription factor, E2A, alters chromatin structure to promote recombination in the Igk locus;5) use gene targeting to delete a CTGF ..
  17. TCF3: A Wnt Pathway Effector in Pluripotent Stem Cell Self-Renewal
    Marian L Waterman; Fiscal Year: 2013
    ..One member, TCF3, is a transcription factor that in mouse ESCs (mESCs) represses expression of master pluripotency factors Oct4, ..
  18. E2A/HEB TRANSCRIPTION FACTORS IN T CELL DEVELOPMENT
    Yuan Zhuang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): E2A/HEB MEDIATED TRANSCRIPTIONAL REGULATION IN T CELL DEVELOPMENT Abstract A cardinal feature of T lymphocytes is their ability to provide antigen specific immune responses to foreign pathogens while ..
  19. Transcriptional regulation of immunological memory
    Laura Shaw; Fiscal Year: 2013
    ..One particular E protein, E2A, is known to be dysregulated in aged mice, resulting in a decreased immune response to infection...
  20. Transcriptional Control of Hematopoiesis
    BARBARA LYNNE KEE; Fiscal Year: 2013
    ..We have determined that the E proteins encoded by the E2A gene are required for proper maintenance of HSCs and they support the initial specification of multipotent ..
  21. Novel transgenic mice for tracing and electrophysiology of stressed neurons
    Jeffrey C Petruska; Fiscal Year: 2013
    ..e., those expressing ChR2). ..
  22. Aging of Early B Cell Precursors
    David M Allman; Fiscal Year: 2010
    ..Finally, in Aim 4, we will define the role of the E2a and EBF transcription factors in the age-associated loss of very early B-lineage progenitors...
  23. Regulation of cyclin D3 in B lymphocyte development
    Marcus R Clark; Fiscal Year: 2013
    ..Igk transcription is induced by ERK- mediated suppression of the Id proteins, and derepression of E2A, while induction of Aiolos silences Ccnd3 leading to cell cycle exit...
  24. Wnt Pathway Regulation of Lens Polarity
    Richard A Lang; Fiscal Year: 2013
    ..By contrast, when the Wnt pathway transcription factor Tcf3 is deleted conditionally in early lens, there is an opposite response where fiber cells fail to differentiate...
  25. Biology and Mechanism of the Single Subunit RNA Polymerases
    RUI J SOUSA; Fiscal Year: 2012
    ..These studies will advance our understanding of fundamental mechanisms of macromolecular complex assembly and of transcription processes in the mitochondrial and nuclear compartments of eukaryotic cells. ..
  26. Regulation of skin epithelial stem cell homeostasis
    Hoang Nguyen; Fiscal Year: 2013
    ..b>Tcf3 and Tcf4 are HMG transcription factor members that either can activate gene transcription when bound to 2-catenin, ..
  27. Pre-B cell receptor signaling in acute lymphoblastic leukemia
    MARKUS MUSCHEN; Fiscal Year: 2013
    ..studies for this proposal on 148 cases of pre-B cell-derived human ALL, we found that ALL cells carrying an E2A-PBX1- or MYC- gene rearrangement are -like normal pre-B cells- highly selected for the expression of a functional ..
  28. Wnt Signaling in Hematopoietic Stem Cell Specification and Leukemia
    WILSON KENDRICK CLEMENTS; Fiscal Year: 2013
    ..To test these possibilities, I will generate transgenic animals in which wnt16, ror1, and the oncogene E2A-PBX1, which has previously been associated with WNT16-directed disease progression in pre-B-ALL, are expressed in B-..
  29. Inactivation of RUNX3 by Helicobacter pylori and gastric cancer
    Lin Feng Chen; Fiscal Year: 2013
    ..These cell-based and in vivo animal experiments will provide new insights into the role of H. pylori CagA in the pathogenesis of H. pylori and give a better understanding of the role RUNX3 plays as a tumor suppressor in gastric cancer. ..
  30. Regulation of TCF3 by Wnt signaling
    Sergei Y Sokol; Fiscal Year: 2013
    ..This proposal aims to study the regulation of TCF3, a transcriptional repressor which has been implicated in Wnt signaling during cell differentiation and ..
  31. Targeting Retroviral DNA Integration: Role of Host Cell Proteins
    Rene Daniel; Fiscal Year: 2009
    ....
  32. PPAR-gamma Signaling in Normal Pilosebaceous Units and in Scarring Alopecia
    Pratima Karnik; Fiscal Year: 2013
    ..We show that the expression of stem cell markers (LGR5, LHX2, SOX9, TCF3) is decreased and that cytokines, growth factors and tissue remodeling genes are upregulated in LPP and in the PPAR ..
  33. GENETIC AND BIOCHEMICAL ANALYSIS OF AD5 REGION 2
    GEOFFREY KITCHINGMAN; Fiscal Year: 1992
    ..RNAs will be synthesized in vitro, binding to various regions of E2A and other mRNAs measured by filter binding assays, and the effect of the DBP on the in vitro translation of these ..
  34. ROLE OF PBX AND PRE B ACUTE LYMPHOBLASTOID LEUKEMIA
    Cornelis Murre; Fiscal Year: 2004
    ..in pre-B ALL, the pbx-1 N-terminal domain has been replaced with a transactivation domain derived from E2A, normally a helix-loop-helix protein...
  35. The role of E-proteins in B cell development
    Cornelis Murre; Fiscal Year: 2013
    ..Members of the E-proteins include E12, E47, E2-2 and HEB...
  36. Embryonic Stem Cell Properties in Cancer
    Bradley J Merrill; Fiscal Year: 2012
    ..One Tcf protein, Tcf3, is expressed in several different types of stem cells (hematopoietic, neural, embryonic, hair follicle) that are ..
  37. Correlates of protection against SIV/SHIV challenge
    Hildegund C J Ertl; Fiscal Year: 2010
    ..vaccines by generating and testing 2nd generation E1-deleted Ad vectors that will have an additional deletion of E2a to reduce synthesis of the highly immunogenic structural antigens of Ad vectors...
  38. Tcf3 function in spinal progenitor maintenance
    Richard I Dorsky; Fiscal Year: 2011
    ..This work will investigate the role of a specific transcriptional regulator, Tcf3, in maintenance of zebrafish spinal cord progenitors...
  39. Molecular Pathways to T-Lineage Commitment
    Cornelis Murre; Fiscal Year: 2009
    ..Recently we have isolated E2A-deficient hematopoietic progenitor cells that can be grown long term in vitro...
  40. Mechanism(s) of TAL1- and NOTCH1-mediated Leukemogenesis
    MICHELLE ALICE KELLIHER; Fiscal Year: 2013
    ..it remains unknown whether human T-ALL growth can be limited in vivo by inhibiting only NOTCH1 or whether the TAL1/E2A oncogenic pathway will also need to be targeted...
  41. HEPATOCYTE GENE THERAPY FOR CITRULLINEMIA
    Brendan Lee; Fiscal Year: 2000
    ..In addition, new adenoviral vectors mutated in the E2a and E4 domains and designed to minimize adenoviral protein expression and host antigen presentation will be tested.
  42. E2A as a Tumor Suppressor
    Cornelis Murre; Fiscal Year: 2013
    ..Members of the E-proteins include E12, E47, E2-2 and HEB...
  43. Wnt Signaling in Xenopus Embryonic Development
    Sergei Y Sokol; Fiscal Year: 2010
    ..The proposal focuses on the analysis of specific Wnt-dependent phosphorylation of TCF3, a transcriptional repressor, identified in preliminary studies...
  44. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
    ..Dissociation Inhibitor), the LH domain from PDZ-RhoGEF, and the BH domain from GRAF, show that K2A (Lys to Ala) and E2A (Glu to Ala) single and multiple mutants crystallize much more readily than wild-type protein...
  45. Immunotherapy of CLL with lentiviral expressed CD40-ligand
    Boro Dropulic; Fiscal Year: 2006
    ..All vectors will contain the Drosophila melanogaster dNK-B10 gene using the E2A cleavage peptide sequence which will increase vector's safety...
  46. GAMMA INTERFERON--REGULATORY AND ANTITUMOR EFFECTS
    Howard Johnson; Fiscal Year: 2001
    ..4) Determine if IFN-gamma-R beta chain undergoes endocytosis and nuclear translocation after IFN-gamma binds to receptor complex and the significance of translocation/non-translocation as it relates to signal transduction. ..
  47. Radioprotective Role of Slug in Bone Marrow Cells
    Wen Shu Wu; Fiscal Year: 2005
    ..Slug was identified as a functional target of the E2A-HLF fusion oncoprotein in pro-B-cell acute leukemias, in which the E2A-HLF fusion gene was generated by the t(17:19)..
  48. Mechanism of T cell lymphoma in E protein deficiency
    Xiao Hong Sun; Fiscal Year: 2009
    ..two basic helix-loop-helix (bHLH) proteins capable of forming heterodimers with other bHLH E proteins such as E2A and HEB...
  49. Genetic dissection of Sle2 contribution to SLE pathogenesis
    Laurence Morel; Fiscal Year: 2010
    ..S/e2 regions, Sle2a, Sle2b, and S/e2c, that affect the size of the B-1a cell pool, but only two of them, S/e2a and Sle2b contribute to autoimmune pathogenesis...
  50. Regulation of immunoglobulin class switch in senescent humans
    Bonnie Blomberg; Fiscal Year: 2007
    ..studies will establish whether the decreases in Ig class switch, AID (activation-induced cytidine deaminase) and E2A we have seen in age-stimulated B lymphocytes in mice is recapitulated in humans, and will then focus on the ..
  51. Regulation of Insulin Gene Transcription by Coactivators
    Melissa Thomas; Fiscal Year: 2004
    ..We have identified a novel PDZ-domain protein, designated Bridge-1, that interacts with E2A transcription factors as a coactivator of insulin gene transcription...
  52. INTERPLAY OF E PROTEINS AND CYCLIN D1 DEPENDENT KINASES
    DONALD KOHTZ; Fiscal Year: 2001
    ..The E proteins (such as the products of the E2A gene) form homodimers and heterodimers with other bHLH proteins that are important in reguiating tissue-speccic ..
  53. TARGETING OF TRANSLOCATION TRANSCRIPTS IN LEUKEMIA
    Asad Bashey; Fiscal Year: 2001
    ..focus upon the pre-clinical development of ribozymes and RNAse P external guide sequences directed against the E2A-PBX1 and BCR-ABL chimeric transcripts in human leukemia...
  54. FUSION GENES IN LEUKEMIA--DETERMINING SIGNIFICANCE
    Mignon Loh; Fiscal Year: 2003
    ..The third specific aim is to apply quantitative RT-PCR techniques to pediatric leukemias associated with the E2A/PBX1 gene rearrangement...
  55. The Molecular Mechanism for E2A Tumor Suppressor
    Yin Lin; Fiscal Year: 2009
    ..Previous studies and preliminary data shown here suggest that E2A likely functions as a tumor suppressor, in part, by regulating cell growth and cellular proliferation...
  56. REGULATION OF P21 (WAF1/C1P1) BY E2A
    Michael Streiff; Fiscal Year: 2001
    ..which is strongly associated with human leukemias, encodes three basic-helix-loop-helix transcription factors (E12, E47, E2-5) which have an established role in the regulation of growth and differentiation in mammalian cells...
  57. Regulation of Primary Human Beta Cell Culture by E2A1-14
    John Choi; Fiscal Year: 2006
    ..Approximately 5- 11% of pediatric pB-ALLs have chromosomal translocations involving the E2A gene, leading to the expression of oncogenic E2A fusion proteins...
  58. PROTEOLYSIS OF LEUKEMIA--RELATED TRANSCRIPTION FACTORS
    Gregory Kato; Fiscal Year: 2000
    ..in childhood leukemia is the E2A gene, which encodes the basic-helix-loop-helix transcription factors E12 and E47. These nearly ubiquitously expressed proteins have a demonstrated growth suppressive role in mammalian cells...
  59. Identification and Characterization of Tcf3 target genes
    Hoang Nguyen; Fiscal Year: 2005
    Several evidence suggest that the transcription factor Tcf3 might play a key role in maintaining skin stem cell properties. An important step in exploring Tcf3's functions in skins will be to identify and characterize its target genes...
  60. DEGRADATION OF E12/E47 IN SMOOTH MUSCLE CELLS
    Gordon Huggins; Fiscal Year: 2002
    ..They are expressed in VSMC. The applicant cloned a novel ubiquitin conjugating enzyme, UBC-E2A, directed against the E12 and E47...
  61. Tcf Function in Spinal Cord Patterning
    RICHARD DORSKY; Fiscal Year: 2009
    ..First, we will test whether Tcf7 and Tcf3 are required for the expression of spinal progenitor domain-specific genes...
  62. TRANSCRIPTIONAL DETERMINANTS OF B LYMPHOCYTES
    THOMAS KADESCH; Fiscal Year: 2002
    ..Specifically, the investigator will characterize the E2A proteins (E12 and E47) by identifying the cause of their activation in B cells and by exploring the consequences of that activation...
  63. Poor competition in hematopoietic stem/progenitor pools favors tumorigenesis
    James DeGregori; Fiscal Year: 2009
    ..To test our hypothesis, we will introduce the oncogenic Bcr-Abl and E2a-Hlf translocation products into mouse BM stem cells using retroviral transduction, and then reconstitute ..
  64. REGULATION OF THE ID GENES IN LYMPHOCYTE DEVELOPMENT
    Xiao Hong Sun; Fiscal Year: 1999
    ..helix protein, is a naturally occurring dominant negative inhibitor of helix-loop helix E proteins, which include E2A, E2-2 and HEB. Id-1 is expressed in progenitor lymphocytes and is downregulated during differentiation...