TBX22

Summary

Gene Symbol: TBX22
Description: T-box 22
Alias: ABERS, CLPA, CPX, TBXX, dJ795G23.1, T-box protein 22, T-box transcription factor TBX22
Species: human

Top Publications

  1. ncbi Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells
    G B Zavilgelsky
    State Scientific Center of the Russian Federation GosNIIgenetika, Moscow, 117545, Russia
    Biochemistry (Mosc) 67:986-92. 2002
  2. ncbi Lactococcus lactis glyceraldehyde-3-phosphate dehydrogenase gene, gap: further evidence for strongly biased codon usage in glycolytic pathway genes
    M R Cancilla
    Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia
    Microbiology 141:1027-36. 1995
  3. ncbi An update on the aetiology of orofacial clefts
    F K Wong
    Faculty of Dentisty, The University of Hong Kong, Pokfulam Road, Hong Kong
    Hong Kong Med J 10:331-6. 2004
  4. ncbi ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells
    J G Thomas
    Department of Chemical Engineering, University of Washington, Seattle 98195, USA
    Mol Microbiol 36:1360-70. 2000
  5. ncbi RNA polymerase of Aquifex pyrophilus: implications for the evolution of the bacterial rpoBC operon and extremely thermophilic bacteria
    H P Klenk
    Georg August Universitat Gottingen, Institut fur Mikrobiologie und Genetik, Grisebachstrabetae 8, 37077 Gottingen, Germany
    J Mol Evol 48:528-41. 1999
  6. ncbi Peptidase activity of the Escherichia coli Hsp31 chaperone
    Abderrahim Malki
    Stress Molecules, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, 75005 Paris, France
    J Biol Chem 280:14420-6. 2005
  7. ncbi Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26 S proteasome
    M Kessel
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 250:587-94. 1995
  8. ncbi Regulated degradation of chromosome replication proteins DnaA and CtrA in Caulobacter crescentus
    Boris Gorbatyuk
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Room 506, Montreal, Quebec, H3A 2B4, Canada
    Mol Microbiol 55:1233-45. 2005
  9. ncbi Inhibition of tumor invasion and metastasis by a novel lysophosphatidic acid (cyclic LPA)
    M Mukai
    Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan
    Int J Cancer 81:918-22. 1999
  10. pmc Contribution of conserved ATP-dependent proteases of Campylobacter jejuni to stress tolerance and virulence
    Marianne Thorup Cohn
    Department of Veterinary Pathobiology, Faculty of Life Sciences, Stigbøjlen 4, DK 1870 Frederiksberg C, Denmark
    Appl Environ Microbiol 73:7803-13. 2007

Research Grants

Scientific Experts

Detail Information

Publications116 found, 100 shown here

  1. ncbi Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells
    G B Zavilgelsky
    State Scientific Center of the Russian Federation GosNIIgenetika, Moscow, 117545, Russia
    Biochemistry (Mosc) 67:986-92. 2002
    The role of chaperones Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA-ClpB-ClpX) in refolding of thermoinactivated luciferase from the marine bacterium Photobacterium fischeri and the terrestrial bacterium Photorhabdus luminescens has been ..
  2. ncbi Lactococcus lactis glyceraldehyde-3-phosphate dehydrogenase gene, gap: further evidence for strongly biased codon usage in glycolytic pathway genes
    M R Cancilla
    Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia
    Microbiology 141:1027-36. 1995
    ..The 3' end of a clpA homologue was identified in the sequence upstream of ORF156. The location of gap on the L...
  3. ncbi An update on the aetiology of orofacial clefts
    F K Wong
    Faculty of Dentisty, The University of Hong Kong, Pokfulam Road, Hong Kong
    Hong Kong Med J 10:331-6. 2004
    ..To review recent data on the aetiology of cleft lip and palate...
  4. ncbi ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells
    J G Thomas
    Department of Chemical Engineering, University of Washington, Seattle 98195, USA
    Mol Microbiol 36:1360-70. 2000
    ..A number of additional proteins, including ClpA, ClpB, HtpG and IbpA/B, act as molecular chaperones in vitro, but their function in cellular protein folding ..
  5. ncbi RNA polymerase of Aquifex pyrophilus: implications for the evolution of the bacterial rpoBC operon and extremely thermophilic bacteria
    H P Klenk
    Georg August Universitat Gottingen, Institut fur Mikrobiologie und Genetik, Grisebachstrabetae 8, 37077 Gottingen, Germany
    J Mol Evol 48:528-41. 1999
    ..RNA polymerase subunits beta and beta' (rpoBC), alanyl-tRNA synthetase (alaS), and subunit A of proteinase Clp (clpA). Enzymatic activity and extreme thermostability of purified A. pyrophilus RNA polymerase were verified...
  6. ncbi Peptidase activity of the Escherichia coli Hsp31 chaperone
    Abderrahim Malki
    Stress Molecules, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, 75005 Paris, France
    J Biol Chem 280:14420-6. 2005
    ..The ClpA component of the ClpAP protease, the chaperone GroEL, elongation factor EF-Tu, and tryptophanase were all found to ..
  7. ncbi Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26 S proteasome
    M Kessel
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 250:587-94. 1995
    ..coli, consists of a proteolytic component, ClpP, in association with an ATP-hydrolyzing, chaperonin-like component, ClpA. To provide a structural basis for understanding the regulation and mechanism of action of Clp protease, we have ..
  8. ncbi Regulated degradation of chromosome replication proteins DnaA and CtrA in Caulobacter crescentus
    Boris Gorbatyuk
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Room 506, Montreal, Quebec, H3A 2B4, Canada
    Mol Microbiol 55:1233-45. 2005
    ..However, a dominant-negative clpX allele that blocks CtrA degradation, even when combined with a clpA null allele, did not decrease DnaA degradation...
  9. ncbi Inhibition of tumor invasion and metastasis by a novel lysophosphatidic acid (cyclic LPA)
    M Mukai
    Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan
    Int J Cancer 81:918-22. 1999
    ..acid (PHYLPA), first isolated from myxoamoebae of Physarum polycephalum, and its synthetic derivatives (cLPA) were tested for their ability to inhibit tumor cell invasion and metastasis...
  10. pmc Contribution of conserved ATP-dependent proteases of Campylobacter jejuni to stress tolerance and virulence
    Marianne Thorup Cohn
    Department of Veterinary Pathobiology, Faculty of Life Sciences, Stigbøjlen 4, DK 1870 Frederiksberg C, Denmark
    Appl Environ Microbiol 73:7803-13. 2007
    ..Following inactivation of the ATPase genes clpA and clpX, only the clpX mutant displayed the same heat sensitivity as the clpP mutant, indicating that the ClpXP ..
  11. pmc Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32)
    M Kitagawa
    Institute for Virus Research, Kyoto University, Japan
    J Bacteriol 173:4247-53. 1991
    ..at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E...
  12. ncbi Human genetic factors in nonsyndromic cleft lip and palate: an update
    Francesco Carinci
    Department of D M C C C, Section of Maxillofacial Surgery, University of Ferrara, Corso, Giovecca 203, 44100 Ferrara, Italy
    Int J Pediatr Otorhinolaryngol 71:1509-19. 2007
    ..In addition, MTHFR, TGF-beta3, and RARalpha play a role in cleft onset. In CPI one gene has been identified (TBX22) at present, but others are probably involved...
  13. pmc A role for the Clp protease in activating Mu-mediated DNA rearrangements
    J A Shapiro
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
    J Bacteriol 175:2625-31. 1993
    ..The clpA::Tn10 mutation, which removes a regulatory subunit of Clp protease, altered the timing of Mu activity in both ..
  14. ncbi Distinctive roles of the two ATP-binding sites in ClpA, the ATPase component of protease Ti in Escherichia coli
    J H Seol
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea
    J Biol Chem 270:8087-92. 1995
    b>ClpA is the ATPase component of the ATP-dependent protease Ti (Clp) in Escherichia coli and contains two ATP-binding sites...
  15. doi Tbx22 expressions during palatal development in fetuses with glucocorticoid-/alcohol-induced C57BL/6N cleft palates
    Soung Min Kim
    Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Seoul, Korea
    J Craniofac Surg 20:1316-26. 2009
    T-box transcription factor 22 (Tbx22) belongs to the T-box family of transcription factors and was originally found using an in silico approach to identify new genes in the human Xq12-Xq21 region...
  16. pmc The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat
    T E Shrader
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 175:4364-74. 1993
    ..The aat gene is located approximately 1 kb from clpA, which encodes a subunit of ATP-dependent protease Clp...
  17. ncbi Molecular properties of ClpAP protease of Escherichia coli: ATP-dependent association of ClpA and clpP
    M R Maurizi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 37:7778-86. 1998
    The ClpAP protease from Escherichia coli consists of the ATP-binding regulatory component, ClpA (subunit Mr 84 165), and the proteolytic component, ClpP (subunit Mr 21 563)...
  18. pmc The Mn1 transcription factor acts upstream of Tbx22 and preferentially regulates posterior palate growth in mice
    Wenjin Liu
    Department of Biomedical Genetics and Center for Oral Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Development 135:3959-68. 2008
    ..Extensive analyses of palatal gene expression in wild-type and Mn1(-/-) mutant mice identified Tbx22, the mouse homolog of the human X-linked cleft palate gene, as a putative downstream target of Mn1 transcriptional ..
  19. pmc Binding of the ClpA unfoldase opens the axial gate of ClpP peptidase
    Grégory Effantin
    Laboratory of Structural Biology Research, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:14834-40. 2010
    ..ability of ClpP to process folded protein substrates depends on its being partnered by an AAA+ ATPase/unfoldase, ClpA or ClpX...
  20. ncbi The N-terminal substrate-binding domain of ClpA unfoldase is highly mobile and extends axially from the distal surface of ClpAP protease
    Takashi Ishikawa
    Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892 8025, USA
    J Struct Biol 146:180-8. 2004
    ClpAP is a barrel-like complex consisting of hexameric rings of the ClpA ATPase stacked on the double heptameric ring of ClpP peptidase. ClpA has two AAA+ domains (Dl and D2) and a 153-residue N-domain...
  21. pmc Role of Clp protease subunits in degradation of carbon starvation proteins in Escherichia coli
    K Damerau
    Nelson Biology Laboratories, Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059
    J Bacteriol 175:53-63. 1993
    ..During starvation, mutants lacking either the ClpA or ClpP subunit of the ATP-dependent Clp protease showed a partial reduction in the degradation of starvation ..
  22. pmc Synchrotron protein footprinting supports substrate translocation by ClpA via ATP-induced movements of the D2 loop
    Jen Bohon
    Center for Proteomics and Center for Synchrotron Biosciences, Case Western Reserve University, Cleveland, OH 44106, USA
    Structure 16:1157-65. 2008
    Synchrotron X-ray protein footprinting is used to study structural changes upon formation of the ClpA hexamer...
  23. ncbi The cyanobacterium Synechococcus sp. PCC 7942 possesses a close homologue to the chloroplast ClpC protein of higher plants
    A K Clarke
    Department of Plant Physiology, University of Umea, Sweden
    Plant Mol Biol 31:721-30. 1996
    ..Using degenerate PCR primers specific for the two distinct ATP-binding domains characteristic of all ClpA-C proteins, partial sequences homologous to clpC from Synechococcus were also identified in five other ..
  24. doi ClpP hydrolyzes a protein substrate processively in the absence of the ClpA ATPase: mechanistic studies of ATP-independent proteolysis
    Laura D Jennings
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:11536-46. 2008
    ..In the case of the bacterial ATP-dependent protease ClpAP, ATP hydrolysis by the ClpA component has been proposed to be required for processive proteolysis of full-length protein substrates...
  25. pmc Local and global mobility in the ClpA AAA+ chaperone detected by cryo-electron microscopy: functional connotations
    Grégory Effantin
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 18:553-62. 2010
    The ClpA chaperone combines with the ClpP peptidase to perform targeted proteolysis in the bacterial cytoplasm. ClpA monomer has an N-terminal substrate-binding domain and two AAA+ ATPase domains (D1 and D2)...
  26. doi Novel two-dimensional DNA gel electrophoresis mapping for characterizing complex bacterial communities in environmental samples
    Guo hua Liu
    Graduate School of Engineering, Yokohama National University, 79 7 Tokiwadai, Hodogaya ku, Yokohama 240 8501, Japan
    J Biosci Bioeng 107:646-51. 2009
    ..a two-dimensional gel electrophoresis (2-DGE) method with a combination of chain-length polymorphism analysis (CLPA) and DGGE analysis, in order to improve the DNA resolution and resolve complex environmental DNA fragments produced ..
  27. ncbi A new component of bacteriophage Mu replicative transposition machinery: the Escherichia coli ClpX protein
    A Mhammedi-Alaoui
    Unité Transposition Bactérienne, Universite Libre de Bruxelles, Rhode St Genese, Belgium
    Mol Microbiol 11:1109-16. 1994
    ..This enzyme is formed by the association between a protease subunit (ClpP) and an ATPase subunit. ClpA, the best characterized of these ATPases, is not required for the degradation of the mutant Mu repressors...
  28. pmc The role of the ClpA chaperone in proteolysis by ClpAP
    J R Hoskins
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 95:12135-40. 1998
    b>ClpA, a member of the Clp/Hsp100 family of ATPases, is a molecular chaperone and, in combination with a proteolytic component ClpP, participates in ATP-dependent proteolysis...
  29. ncbi Isolation and characterization of ClpX, a new ATP-dependent specificity component of the Clp protease of Escherichia coli
    D Wojtkowiak
    Department of Molecular Biology, University of Gdansk, Kladki, Poland
    J Biol Chem 268:22609-17. 1993
    ..The 23,000-Da component (LopP) was identified as the previously characterized ClpP protein, known to complex with ClpA to form the ClpAP, an ATP-dependent protease, capable of degrading casein...
  30. pmc Patterning of palatal rugae through sequential addition reveals an anterior/posterior boundary in palatal development
    Sophie Pantalacci
    Molecular Zoology, Institut de Génomique Fonctionnelle de Lyon, Universite de Lyon, Universite Lyon 1, CNRS, INRA, Ecole Normale Superieure de Lyon, Lyon, France
    BMC Dev Biol 8:116. 2008
    ..However, rugae could be useful as landmarks to monitor anterior/posterior (A/P) palatal growth, and they provide a simple model of mesenchymal-epithelial structures arranged in a serial pattern...
  31. ncbi The two-component, ATP-dependent Clp protease of Escherichia coli. Purification, cloning, and mutational analysis of the ATP-binding component
    Y Katayama
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892
    J Biol Chem 263:15226-36. 1988
    The ATP-binding component (Component II, hereafter referred to as ClpA) of a two-component, ATP-dependent protease from Escherichia coli has been purified to homogeneity. ClpA is a protein with subunit Mr 81,000...
  32. pmc ClpB in a cyanobacterium: predicted structure, phylogenetic relationships, and regulation by light and temperature
    M Celerin
    Department of Plant Sciences, University of Western Ontario, London, Ontario, Canada N6A 5B7
    J Bacteriol 180:5173-82. 1998
    ..are not unique to p-ClpB; they are present in all ClpB sequences examined and are absent from the ClpB paralogs ClpA, ClpC, ClpX, and ClpY. Small quantities of a 4...
  33. doi The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation
    César Díez-Gil
    Departament de Nanociència Molecular i Materials Orgànics Institut de Ciència de Materials de Barcelona ICMAB CSIC, Bellaterra, 08193 Barcelona, Spain
    Biomaterials 31:5805-12. 2010
    ..In regard to wild type, DnaK(-) and ClpA(-) strains produce inclusion bodies with distinguishable wettability, stiffness and stiffness distribution within ..
  34. pmc Turnover of endogenous SsrA-tagged proteins mediated by ATP-dependent proteases in Escherichia coli
    Mark Lies
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:22918-29. 2008
    ..ClpAP degrades SsrA-tagged proteins slowly even in the absence of SspB, possibly because of interference from ClpA-specific substrates. Lon protease degrades SsrA-tagged proteins at a rate of approximately 0...
  35. doi Autosomal-dominant ankyloglossia and tooth number anomalies
    A C Acevedo
    Oral Care Center for Inherited Diseases, University Hospital of Brasilia, Department of Dentistry, University of Brasilia, Brazil
    J Dent Res 89:128-32. 2010
    ..X-linked cleft palate and is sometimes seen alone due to mutations in the gene encoding the transcription factor TBX22, while knockout of Lgr5 in the mouse results in ankyloglossia...
  36. ncbi Clp P represents a unique family of serine proteases
    M R Maurizi
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892
    J Biol Chem 265:12546-52. 1990
    ..Processing of Clp P appears, therefore, to involve an intermolecular autocatalytic cleavage reaction. Since processing of Clp P occurs in clpA- cells, the autoprocessing activity of Clp P is independent of Clp A.
  37. ncbi Concurrent chaperone and protease activities of ClpAP and the requirement for the N-terminal ClpA ATP binding site for chaperone activity
    M Pak
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 274:19316-22. 1999
    b>ClpA, a member of the Clp/Hsp100 family of ATPases, is both an ATP-dependent molecular chaperone and the regulatory component of ClpAP protease...
  38. ncbi ClpA and ClpP remain associated during multiple rounds of ATP-dependent protein degradation by ClpAP protease
    S K Singh
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    Biochemistry 38:14906-15. 1999
    The Escherichia coli ClpA and ClpP proteins form a complex, ClpAP, that catalyzes ATP-dependent degradation of proteins...
  39. ncbi Mutational analysis demonstrates different functional roles for the two ATP-binding sites in ClpAP protease from Escherichia coli
    S K Singh
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:29537-45. 1994
    b>ClpA, the regulatory subunit of Clp protease from Escherichia coli, has two ATP-binding sites in non-homologous regions of the protein, referred to as domain I and domain II...
  40. ncbi ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes
    Joaquin Ortega
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA
    J Struct Biol 146:217-26. 2004
    ..are composed of a single proteolytic component, ClpP, complexed with either of the two related chaperones, ClpA or ClpX...
  41. pmc Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system
    Carr D Vincent
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Mol Microbiol 85:378-91. 2012
    ..Loss of DotL is dependent on ClpA, a regulator of the cytoplasmic protease ClpP...
  42. doi Structural determinants stabilizing the axial channel of ClpP for substrate translocation
    John Alexopoulos
    Department of Biochemistry and Biomedical Sciences and M G DeGroote Institute for Infectious Diseases Research, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S4K1, Canada
    Mol Microbiol 90:167-80. 2013
    ..ADEPs) antibiotics bind to Escherichia coli ClpP mimicking the interactions that the IGL/F loops in ClpA or ClpX ATPases establish with the hydrophobic pockets surrounding the axial pore of the tetradecamer that the ..
  43. doi E. coli ClpA catalyzed polypeptide translocation is allosterically controlled by the protease ClpP
    Justin M Miller
    Department of Chemistry, The University of Alabama at Birmingham, 1530 Third Avenue South, Birmingham, AL 35294 1240, USA
    J Mol Biol 425:2795-812. 2013
    ..Hexameric ClpA rings associate with one or both faces of the cylindrically shaped tetradecameric ClpP protease...
  44. doi Cleavage of the antitoxin of the parD toxin-antitoxin system is determined by the ClpAP protease and is modulated by the relative ratio of the toxin and the antitoxin
    Elizabeth Diago-Navarro
    Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biologicas CSIC, Madrid, Spain
    Plasmid 70:78-85. 2013
    ..We further show that this protection is correlated with the inability of the ClpA chaperone to access the Kis antitoxin when in complex with Kid toxin...
  45. doi X-linked CHARGE-like Abruzzo-Erickson syndrome and classic cleft palate with ankyloglossia result from TBX22 splicing mutations
    E Pauws
    UCL Institute of Child Health, 30 Guilford Street, London, UK
    Clin Genet 83:352-8. 2013
    X-linked cleft palate (CPX) is caused by mutations in the gene encoding the TBX22 transcription factor and is known to exhibit phenotypic variability, usually involving either a complete, partial or submucous cleft palate, with or ..
  46. ncbi Roles of the N-domains of the ClpA unfoldase in binding substrate proteins and in stable complex formation with the ClpP protease
    Jörg Hinnerwisch
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 280:40838-44. 2005
    The hexameric cylindrical Hsp100 chaperone ClpA mediates ATP-dependent unfolding and translocation of recognized substrate proteins into the coaxially associated serine protease ClpP...
  47. doi Mutational, proteomic and metabolomic analysis of a plant growth promoting copper-resistant Pseudomonas spp
    KeFeng Li
    Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA
    FEMS Microbiol Lett 335:140-8. 2012
    ..CSM1, a mutant disrupted in trpA gene (tryptophan synthase alpha subunit), and CSM2, a mutant disrupted in clpA gene (ATP-dependent Clp protease)...
  48. pmc Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection
    Wentao Guo
    National Reference Laboratory of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, People s Republic of China
    PLoS ONE 7:e43322. 2012
    ..The 11 cDNAs were related to metabolism or degradation of glycolysis (gpmA and pgi) and proteins (clpX, clpA, pepN and fkpB). The atpADG and ubiB genes were associated with ATP biosynthesis and electron transport chain...
  49. pmc Identification of the protease and the turnover signal responsible for cell cycle-dependent degradation of the Caulobacter FliF motor protein
    Björn Grünenfelder
    Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    J Bacteriol 186:4960-71. 2004
    ..Cell cycle-dependent turnover of FliF requires an intact clpA gene, suggesting that the ClpAP protease is required for removal of the MS ring protein...
  50. ncbi Anything a ClpA Can Do, Two ClpAs Can Do Better
    Steven G Burston
    Department of Biochemistry, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, UK
    Structure 17:483-4. 2009
    ..Through the application of a technique they have developed to produce mixed-ring versions of ClpP, Maglica et al. investigate the impact of architecture and allostery on ClpAP proteolytic efficiency...
  51. pmc Distinct genetic alterations in colorectal cancer
    Hassan Ashktorab
    Department of Medicine and Cancer Center, Howard University, College of Medicine, Washington, DC, United States of America
    PLoS ONE 5:e8879. 2010
    ..In this study, we determined genomic copy number aberrations in sporadic CRC tumors from AAs, in order to investigate possible explanations for the observed disparities...
  52. pmc A single ClpS monomer is sufficient to direct the activity of the ClpA hexamer
    Gian Marco De Donatis
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 285:8771-81. 2010
    ClpS is an adaptor protein that interacts with ClpA and promotes degradation of proteins with N-end rule degradation motifs (N-degrons) by ClpAP while blocking degradation of substrates with other motifs...
  53. pmc The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system
    S Gottesman
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    Genes Dev 12:1338-47. 1998
    ..with purified components and required a substrate with a wild-type SsrA tail, the presence of both ClpP and either ClpA or ClpX, and ATP...
  54. ncbi Enzymatic and structural similarities between the Escherichia coli ATP-dependent proteases, ClpXP and ClpAP
    R Grimaud
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:12476-81. 1998
    ..Competition studies showed that ClpA may have a slightly higher affinity (approximately 2-fold) for binding to ClpP...
  55. doi The Escherichia coli ClpA molecular chaperone self-assembles into tetramers
    P Keith Veronese
    Department of Chemistry, The University of Alabama at Birmingham, 1530 3rd Ave S, Birmingham, Alabama 35294 1240, USA
    Biochemistry 48:9221-33. 2009
    The Escherichia coli ATP-dependent protease, ClpAP, is composed of the hexameric ATPase/protein-unfoldase, ClpA, and the tetradecameric proteolytic component, ClpP...
  56. pmc Tbx22null mice have a submucous cleft palate due to reduced palatal bone formation and also display ankyloglossia and choanal atresia phenotypes
    Erwin Pauws
    UCL Institute of Child Health, 30 Guilford Street, WC1N 1EH London, UK
    Hum Mol Genet 18:4171-9. 2009
    ..and ankyloglossia results from loss-of-function mutations in the gene encoding the T-box transcription factor TBX22. Further studies show that TBX22 mutations are also found in around 5% of non-syndromic cleft palate patients...
  57. doi Studying chaperone-proteases using a real-time approach based on FRET
    Kristina Kolygo
    ETH Zurich, Institute of Molecular Biology and Biophysics, Switzerland
    J Struct Biol 168:267-77. 2009
    ..Our data for ClpX and ClpA support the mechanical unfolding mode of action proposed for these chaperones...
  58. ncbi At sixes and sevens: characterization of the symmetry mismatch of the ClpAP chaperone-assisted protease
    F Beuron
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, 20892, USA
    J Struct Biol 123:248-59. 1998
    ClpAP, a typical energy-dependent protease, consists of a proteolytic component (ClpP) and a chaperone-like ATPase (ClpA)...
  59. pmc Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22
    P A Jezewski
    Department of Cytokine Biology, The Forsyth Institute, and Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA
    Dev Dyn 238:1605-12. 2009
    Mutations in human TBX22 cause X-linked cleft palate with ankyloglossia syndrome (CPX; OMIM 303400)...
  60. pmc Conditional stability of the HemA protein (glutamyl-tRNA reductase) regulates heme biosynthesis in Salmonella typhimurium
    L Wang
    West Virginia University Health Sciences Center, Morgantown, West Virginia 26506, USA
    J Bacteriol 181:1211-9. 1999
    ..coli. Each single mutant shows only a small effect. The ClpA chaperone, but not ClpX, is required for ClpP-dependent HemA turnover...
  61. pmc Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions
    Douglas J Kojetin
    Department of Molecular Genetics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    J Mol Biol 387:639-52. 2009
    ..The electrostatic surface of N-ClpCR differs substantially from the N-domain of Escherichia coli ClpA and ClpB, suggesting that the electrostatic surface characteristics of HSP100/Clp N-domains may play a role in ..
  62. doi ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway
    Ronny Schmidt
    Zentrum für Molekulare Biologie Heidelberg ZMBH, DKFZ ZMBH Alliance, Universitat Heidelberg, Heidelberg, Germany
    Mol Microbiol 72:506-17. 2009
    ..coli the adaptor protein ClpS directly interacts with destabilizing N-terminal residues and transfers them to the ClpA/ClpP proteolytic complex for degradation...
  63. ncbi ClpP participates in the degradation of misfolded protein in Lactococcus lactis
    D Frees
    Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
    Mol Microbiol 31:79-87. 1999
    ..In Escherichia coli, ClpP is the proteolytic component of a large complex also containing either the ClpA or the ClpX ATPases...
  64. ncbi Site-directed mutagenesis of the Cys residues in ClpA, the ATPase component of protease Ti (ClpAP) in Escherichia coli
    J H Seol
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea
    Biol Chem 378:1205-9. 1997
    ..been shown to be inhibited by sulfhydryl blocking agents, such as N-ethylmaleimide (NEM), when preincubated with ClpA but not with ClpP...
  65. doi Molecular mechanism of polypeptide translocation catalyzed by the Escherichia coli ClpA protein translocase
    Burki Rajendar
    The University of Alabama at Birmingham, Department of Chemistry, 1530 3rd Avenue South, Birmingham, AL 35294 1240, USA
    J Mol Biol 399:665-79. 2010
    ..Here, we demonstrate that Escherichia coli ClpA, in the absence of the proteolytic component ClpP, processively and directionally steps along the polypeptide ..
  66. ncbi Modulation of intracellular protein degradation by SSB1-SIS1 chaperon system in yeast S. cerevisiae
    M Ohba
    Mitsubishi Kasei Institute of Life Sciences, Machida shi, Tokyo, Japan
    FEBS Lett 409:307-11. 1997
    In prokaryotes, DnaK-DnaJ chaperon is involved in the protein degradation catalyzed by proteases La and ClpA/B complex as shown in E. coli...
  67. ncbi The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate
    S Gottesman
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 265:7886-93. 1990
    The clpA gene, which codes for the ATP-binding subunit of the ATP-dependent Clp protease of Escherichia coli, has been sequenced...
  68. ncbi Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli
    M R Maurizi
    Laboratory of Molecular Biology, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
    J Biol Chem 265:12536-45. 1990
    ..coli map, close to that for the ATP-dependent Lon protease of E. coli and far from the gene for clpA. Primer extension experiments indicate that transcription initiates immediately upstream of the coding region for ..
  69. ncbi Expression of a putative ATPase suppresses the growth defect of a yeast potassium transport mutant: identification of a mammalian member of the Clp/HSP104 family
    F Périer
    Department of Biological Sciences, University of California, Santa Barbara 93106
    Gene 152:157-63. 1995
    ..domain of SKD3 has 57-64% similarity (30-40% identity) with members of the Clp/HSP104 family, including the ClpA regulatory subunit of the Clp protease and S. cerevisiae heat-shock protein 104...
  70. ncbi The 65-kDa protein derived from the internal translational initiation site of the clpA gene inhibits the ATP-dependent protease Ti in Escherichia coli
    J H Seol
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea
    J Biol Chem 269:29468-73. 1994
    The clpA gene that encodes the ATPase subunit of the ATP-dependent protease Ti (Clp) in Escherichia coli contains a putative internal translational initiation site...
  71. ncbi Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins
    S J Lee
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854
    Mol Microbiol 11:833-9. 1994
    ..In addition, a DNA sequence upstream of the clpA gene at 19 min published elsewhere contains an open reading frame for a 74-residue protein with 45% identity to ..
  72. ncbi The 65-kDa protein derived from the internal translational start site of the clpA gene blocks autodegradation of ClpA by the ATP-dependent protease Ti in Escherichia coli
    J H Seol
    Department of Molecular Biology, College of Natural Sciences, Seoul National University, South Korea
    FEBS Lett 377:41-3. 1995
    The ATP-dependent protease Ti consists of two different components: ClpA containing ATP-cleaving sites and ClpP having serine active sites for proteolysis...
  73. doi Acyl depsipeptide (ADEP) resistance in Streptomyces
    Myriam Gominet
    Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram Positif, CNRS URA 2172, F 75015 Paris, France
    Microbiology 157:2226-34. 2011
    ..Access of protein to the ClpP proteolytic chamber is therefore facilitated and its cohort regulatory ATPases (ClpA, ClpC, ClpX) are not required...
  74. doi Prevalence and characterization of Campylobacter jejuni isolated from pasture flock poultry
    Irene Hanning
    Dept of Food Science, Center for Food Safety Inst of Food Science and Engineering, Univ of Arkansas, Fayetteville, AR 72704, USA
    J Food Sci 75:M496-502. 2010
    ..flaC, cadF, dnaJ, racR, cbrR), invasion (virB11, ciaB, pldA), protection against harsh conditions (sodB, htrA, clpA), toxin production (cdtA, cdtB, cdtC), siderophore transport (ceuE), and ganglioside mimicry (wlaN)...
  75. ncbi A new method to evaluate the unfolding activity of chaperone unit ClpA based on Fe-S cluster disruption
    Takashi Ohgita
    Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto, Japan
    Chem Pharm Bull (Tokyo) 59:657-61. 2011
    ..To verify this hypothesis, the absorption spectrum of Fd complexed with ClpA, the chaperone unit of ClpAP, was measured...
  76. doi Application of the sequential n-step kinetic mechanism to polypeptide translocases
    Aaron L Lucius
    Department of Chemistry, The University of Alabama at Birmingham, Birmingham, Alabama, USA
    Methods Enzymol 488:239-64. 2011
    ..coli ClpA polypeptide translocase...
  77. pmc Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii
    Gabriele Margos
    University of Bath, Department of Biology and Biochemistry, Claverton Down, Bath BA2 7AY, UK
    Ticks Tick Borne Dis 1:151-8. 2010
    ..We amplified internal fragments of 8 housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) located on the main linear chromosome by polymerase chain reaction...
  78. doi Effect of temperature on the self-assembly of the Escherichia coli ClpA molecular chaperone
    P Keith Veronese
    Department of Chemistry, The University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, Alabama 35294 1240, United States
    Biochemistry 49:9820-9. 2010
    ..ClpAP is composed of a tetradecameric serine protease, ClpP (21.6 kDa monomer), and the ATPase/protein unfoldase ClpA (84.2 kDa monomer)...
  79. doi MTHFR and MSX1 contribute to the risk of nonsyndromic cleft lip/palate
    Triin Jagomagi
    Department of Stomatology, Faculty of Medicine, University of Tartu, Tartu, Estonia
    Eur J Oral Sci 118:213-20. 2010
    ..This study provides further evidence implicating MSX1 and MTHFR in the etiology of nonsyndromic CL/P across different populations...
  80. pmc Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis
    J M Flynn
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 98:10584-9. 2001
    ..analysis of the ssrA tag reveals independent, but overlapping determinants for its interactions with ClpX, ClpA, and SspB, a specificity-enhancing factor for ClpX...
  81. doi Role of a conserved pore residue in the formation of a prehydrolytic high substrate affinity state in the AAA+ chaperone ClpA
    Mary E Farbman
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:13497-505. 2008
    The AAA+ protease ClpAP, consisting of the ClpA chaperone and the ClpP protease, processively unfolds and translocates its substrates into its proteolytic core, where they are cleaved...
  82. ncbi Importance of heptameric ring integrity for activity of Escherichia coli ClpP
    M W Thompson
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892, USA
    Eur J Biochem 258:923-8. 1998
    ..Radiation target sizes determined for small peptide hydrolysis, for ClpA activated and nucleotide-activated oligopeptide cleavage, and for ClpA-activated ATP-dependent protein degradation ..
  83. pmc Lon and Clp family proteases and chaperones share homologous substrate-recognition domains
    C K Smith
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 96:6678-82. 1999
    ..Fragments corresponding to these sequences are stably and independently folded for Lon, ClpA, and ClpY. The corresponding regions from ClpB and ClpX are unstable...
  84. ncbi Molecular characterization of a new human T-box gene (TBX22) located in xq21.1 encoding a protein containing a truncated T-domain
    F Laugier-Anfossi
    Inserm Unité 491, Faculté de médecine La Timone, 27 Bd Jean Moulin, 13385 Cedex 5, Marseille, France
    Gene 255:289-96. 2000
    ..We show here that this new gene, called TBX22, is composed of seven exons spanning 8.7 kilobases of genomic DNA in Xq21.1...
  85. pmc ClpA mediates directional translocation of substrate proteins into the ClpP protease
    B G Reid
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 98:3768-72. 2001
    ..g., ClpAP, ClpXP, and HslUV in prokaryotes, and the 26S proteasome in eukaryotes. Recent studies of the chaperone ClpA indicate that it mediates ATP-dependent unfolding of substrate proteins and directs their ATP-dependent ..
  86. ncbi Binding and degradation of heterodimeric substrates by ClpAP and ClpXP
    Suveena Sharma
    Laboratory of Molecular Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 280:5449-55. 2005
    b>ClpA and ClpX function both as molecular chaperones and as the regulatory components of ClpAP and ClpXP proteases, respectively...
  87. pmc Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses
    E Ekaza
    Institut National de la Santé et de la Recherche Médicale U 431, Universite Montpellier II, F 34095 Montpellier, France
    J Bacteriol 183:2677-81. 2001
    ..The effects were reversible by complementation. Simultaneous inactivation of clpA and clpB resulted in a mutant that was sensitive to oxidative stress. In B...
  88. ncbi Functional domains of the ClpA and ClpX molecular chaperones identified by limited proteolysis and deletion analysis
    S K Singh
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:29420-9. 2001
    Escherichia coli ClpA and ClpX are ATP-dependent protein unfoldases that each interact with the protease, ClpP, to promote specific protein degradation...
  89. pmc Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter
    Lam Son Phan Tran
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 16:2481-98. 2004
    ..expression of the Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION STRESS 1 (ERD1) gene, which encodes a ClpA (ATP binding subunit of the caseinolytic ATP-dependent protease) homologous protein...
  90. ncbi The T-box transcription factor gene TBX22 is mutated in X-linked cleft palate and ankyloglossia
    C Braybrook
    Institute of Reproductive and Developmental Biology, Imperial College, Hammersmith Campus, Du Cane Road, London W12 0NN, UK
    Nat Genet 29:179-83. 2001
    ..we show that CPX is caused by mutations in the gene encoding the recently described T-box transcription factor TBX22 (ref. 14)...
  91. ncbi Cytoplasmic degradation of ssrA-tagged proteins
    Christopher M Farrell
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Microbiol 57:1750-61. 2005
    ..Levels of ClpA and ClpP increased about threefold during this transition, whereas ClpX, ClpS and SspB levels remained nearly ..
  92. ncbi T-box genes in human disorders
    Elizabeth A Packham
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, UK
    Hum Mol Genet 12:R37-44. 2003
    ..syndrome/TBX3, and more recently DiGeorge syndrome/TBX1, ACTH deficiency/TBX19 and cleft palate with ankyloglossia/TBX22. This review describes the key features of these disorders and the involvement of T-box genes in their phenotype.
  93. pmc Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free
    Monika Slominska
    Department of Cell Biology, Institute for Cancer Research, Montebello, 0310 Oslo, Norway
    Biochem J 370:867-71. 2003
    ..The protein was stabilized by the presence of the chaperones ClpA and ClpX and degraded by their cognate protease ClpP...
  94. ncbi Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA
    Kornelius Zeth
    MPI für Biochemie, Abteilung Membranbiochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Struct Biol 9:906-11. 2002
    ..Generally, the substrate specificity of these machines is determined by chaperone components, such as ClpA. In some cases, however, the specificity is modified by adaptor proteins, such as ClpS. Here we report the 2...
  95. ncbi Isolation and developmental expression analysis of Tbx22, the mouse homolog of the human X-linked cleft palate gene
    Jeffrey O Bush
    Department of Biology, University of Rochester, Rochester, New York 14642, USA
    Dev Dyn 225:322-6. 2002
    Mutations in the TBX22 gene have been identified recently in patients with the X-linked cleft palate and ankyloglossia syndrome, suggesting that the TBX22 transcription factor plays an important role in palate development...
  96. ncbi Cloning and expression analysis of the chick ortholog of TBX22, the gene mutated in X-linked cleft palate and ankyloglossia
    Bénédicte Haenig
    Abt Molekulare Embryologie, Max Planck Institut fur Immunbiologie, Stubeweg 51, 79108 Freiburg, Germany
    Mech Dev 117:321-5. 2002
    ..Several family members have been implicated in human congenital diseases. Recently, mutations in TBX22 were found to cause X-linked cleft palate (CPX and ankyloglossia), a semidominant X-linked disorder affecting ..
  97. ncbi PAX9 and TGFB3 are linked to susceptibility to nonsyndromic cleft lip with or without cleft palate in the Japanese: population-based and family-based candidate gene analyses
    Eisaburo Ichikawa
    Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan
    J Hum Genet 51:38-46. 2006
    ..population, relationships between CL/P or CPO and seven candidate genes (TGFB3, DLX3, PAX9, CLPTM1, TBX10, PVRL1, TBX22) that showed positive associations in other populations and are expressed in the oral/lip region in developing ..
  98. pmc Two peptide sequences can function cooperatively to facilitate binding and unfolding by ClpA and degradation by ClpAP
    Joel R Hoskins
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:909-14. 2006
    ..The ClpA and ClpX ATPases of Escherichia coli generally recognize short amino acid sequences that are located near the N or ..
  99. doi An intrinsic degradation tag on the ClpA C-terminus regulates the balance of ClpAP complexes with different substrate specificity
    Zeljka Maglica
    ETH Zurich, Institute of Molecular Biology and Biophysics, Schafmattstr 20, CH 8093 Zurich, Switzerland
    J Mol Biol 384:503-11. 2008
    ..In Escherichia coli, ClpP interacts with two alternative ATPases, ClpA or ClpX, to form active protease complexes. ClpAP and ClpXP show different but overlapping substrate specificities...
  100. pmc The ClpP N-terminus coordinates substrate access with protease active site reactivity
    Laura D Jennings
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:11031-40. 2008
    ..machines, such as the Escherichia coli protease ClpAP, require regulated interactions between the ATPase component (ClpA) and the protease component (ClpP) for function...

Research Grants38

  1. MOLECULAR GENETIC ANALYSIS OF CRANIOFACIAL DEVELOPMENT
    Rulang Jiang; Fiscal Year: 2009
    ..In addition, we found that the mouse Tbx22 gene, which is orthologous to the human TBX22 gene associated with X-linked cleft palate, exhibits a similar ..
  2. STRUCTURE & INTERACTIONS OF THE CLP PROTEASE SYSTEM: FOLDING & DEGRADATION
    John Flanagan; Fiscal Year: 2002
    ..It consists of the ClpP protease, which by itself is probably inactive on most physiological substrates, and the ClpA family of ATPases, which present substrates to CIpP...
  3. CLP--AN ARCHETYPAL ATP DEPENDENT PROTEASE
    John Flanagan; Fiscal Year: 2001
    ..The Clp protease is composed of a proteolytic core, ClpP and an ATPase component, ClpA, ClpC or ClpX...
  4. Energetics of Unfolding and Translocation by ClpA
    MELVA JAMES; Fiscal Year: 2007
    ..research proposal is to deconvolute the individual mechanistic steps that describe the interaction of the enzyme ClpA with its protein substrates...
  5. MECHANISM OF ACTION OF THE HSP100 CHAPERONE CLPA
    Arthur Horwich; Fiscal Year: 2006
    ..The site of substrate binding on ClpA will be determined by EM analysis of gold-tagged substrate proteins bound to CIpAP complexes...
  6. METHYLATION PROFILING AND RISK OF COLORECTAL CANCER
    Hassan Ashktorab; Fiscal Year: 2007
    ..These studies will help in the detection and profiling of genetic changes in the pathway of CRC in AA. ..
  7. METHYLATION PROFILING AND RISK OF COLORECTAL CANCER
    Hassan Ashktorab; Fiscal Year: 2007
    ..These studies will help in the detection and _rofiling of genetic changes in the pathway of CRC in AA. ..
  8. STRUCTURE AND FUNCTION OF HSP 100 PROTEINS
    Michal Zolkiewski; Fiscal Year: 2005
    ..Characterize interactions between ClpB95 or ClpB80 and the co-chaperones (DnaK, DnaJ, GrpE). 3. Characterize the role of ClpB95 or ClpB80 and the three co-chaperones in the disaggregation and reactivation of luciferase. ..
  9. Mechanisms of Growth Inhibition by Helicobacter Pylor
    Hassan Ashktorab; Fiscal Year: 2003
    ..These studies will help to establish how bacteria can increase apoptosis possibly through generation of ROS and in specific circumstances this may alter one's risk of developing cancer. ..