TAS1R3

Summary

Gene Symbol: TAS1R3
Description: taste receptor, type 1, member 3
Alias: T1R3, sweet taste receptor T1R3, taste receptor type 1 member 3
Species: human

Top Publications

  1. ncbi An amino-acid taste receptor
    Greg Nelson
    Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, California, 92093 0649, USA
    Nature 416:199-202. 2002
  2. ncbi A TAS1R receptor-based explanation of sweet 'water-taste'
    Veronica Galindo-Cuspinera
    Monell Chemical Senses Center, 3500 Market Street, Philadelphia, Pennsylvania 19104, USA
    Nature 441:354-7. 2006
  3. doi The functional involvement of gut-expressed sweet taste receptors in glucose-stimulated secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY)
    R E Steinert
    Clinical Research Center, Department of Biomedicine, Switzerland
    Clin Nutr 30:524-32. 2011
  4. pmc The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor
    Marcel Winnig
    German Institute of Human Nutrition Potsdam Rehbruecke, Department of Molecular Genetics, Arthur Scheunert Allee 114 116, 14558 Nuthetal, Germany
    BMC Struct Biol 7:66. 2007
  5. pmc Different functional roles of T1R subunits in the heteromeric taste receptors
    Hong Xu
    Senomyx, Inc, 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 101:14258-63. 2004
  6. ncbi Expression of the sweet receptor protein, T1R3, in the human liver and pancreas
    Kazumi Taniguchi
    Department of Veterinary Anatomy, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan
    J Vet Med Sci 66:1311-4. 2004
  7. doi The role of the gut sweet taste receptor in regulating GLP-1, PYY, and CCK release in humans
    A C Gerspach
    Phase 1 Research Unit, Department of Biomedicine, University Hospital Basel, Basel, Switzerland
    Am J Physiol Endocrinol Metab 301:E317-25. 2011
  8. ncbi A candidate taste receptor gene near a sweet taste locus
    J P Montmayeur
    Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Neurosci 4:492-8. 2001
  9. ncbi The cysteine-rich region of T1R3 determines responses to intensely sweet proteins
    Peihua Jiang
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 279:45068-75. 2004
  10. ncbi Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste
    Peihua Jiang
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:15238-46. 2005

Research Grants

  1. Mechanisms of sweet transduction in mammalian taste buds
    Nirupa Chaudhari; Fiscal Year: 2007
  2. Mechanisms of sweet transduction in mammalian taste buds
    Nirupa Chaudhari; Fiscal Year: 2010
  3. Role of taste signaling elements in enteroendocrine cells
    Robert F Margolskee; Fiscal Year: 2010
  4. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2007
  5. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2010
  6. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2009
  7. Functional characterization of endocrine taste cells
    Robert Margolskee; Fiscal Year: 2009
  8. Functional characterization of endocrine taste cells
    Robert Margolskee; Fiscal Year: 2009
  9. Functional characterization of endocrine taste cells
    Robert F Margolskee; Fiscal Year: 2010
  10. Functional characterization of endocrine taste cells
    Robert F Margolskee; Fiscal Year: 2011

Scientific Experts

Detail Information

Publications126 found, 100 shown here

  1. ncbi An amino-acid taste receptor
    Greg Nelson
    Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, California, 92093 0649, USA
    Nature 416:199-202. 2002
    ..This receptor, T1R1+3, is a heteromer of the taste-specific T1R1 and T1R3 G-protein-coupled receptors...
  2. ncbi A TAS1R receptor-based explanation of sweet 'water-taste'
    Veronica Galindo-Cuspinera
    Monell Chemical Senses Center, 3500 Market Street, Philadelphia, Pennsylvania 19104, USA
    Nature 441:354-7. 2006
    ..the psychophysical responses-water rinses remove the inhibitor from the heteromeric sweetener receptor TAS1R2-TAS1R3, which activates cells and results in the perception of strong sweetness from pure water...
  3. doi The functional involvement of gut-expressed sweet taste receptors in glucose-stimulated secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY)
    R E Steinert
    Clinical Research Center, Department of Biomedicine, Switzerland
    Clin Nutr 30:524-32. 2011
    ..Enteroendocrine cells are thought to directly sense nutrients via α-gustducin coupled taste receptors (originally identified in the oral epithelium) to modulate the secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY)...
  4. pmc The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor
    Marcel Winnig
    German Institute of Human Nutrition Potsdam Rehbruecke, Department of Molecular Genetics, Arthur Scheunert Allee 114 116, 14558 Nuthetal, Germany
    BMC Struct Biol 7:66. 2007
    ..used isovanillyl sweetener neohesperidin dihydrochalcone activates the human but not the rat sweet receptor TAS1R2+TAS1R3. Analysis of interspecies combinations and chimeras of rat and human TAS1R2+TAS1R3 suggested that the heptahelical ..
  5. pmc Different functional roles of T1R subunits in the heteromeric taste receptors
    Hong Xu
    Senomyx, Inc, 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 101:14258-63. 2004
    ..Surprisingly, the C-terminal transmembrane domain of T1R3 is required for recognizing sweetener cyclamate and sweet taste inhibitor lactisole...
  6. ncbi Expression of the sweet receptor protein, T1R3, in the human liver and pancreas
    Kazumi Taniguchi
    Department of Veterinary Anatomy, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan
    J Vet Med Sci 66:1311-4. 2004
    The expression of T1R3, a taste receptor essential for the perception of sweetness and umami-taste, was examined by immunohistochemistry to determine whether and where it may be localized in the liver and pancreas...
  7. doi The role of the gut sweet taste receptor in regulating GLP-1, PYY, and CCK release in humans
    A C Gerspach
    Phase 1 Research Unit, Department of Biomedicine, University Hospital Basel, Basel, Switzerland
    Am J Physiol Endocrinol Metab 301:E317-25. 2011
    ..Lactisole can inhibit the sweet taste receptor T1R2/T1R3. The objective was to use lactisole as a probe to investigate the physiological role of T1R2/T1R3 by assessing the ..
  8. ncbi A candidate taste receptor gene near a sweet taste locus
    J P Montmayeur
    Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Neurosci 4:492-8. 2001
    ..Here we describe a candidate taste receptor gene, T1r3, that is located at or near the mouse Sac locus, a genetic locus that controls the detection of certain sweet ..
  9. ncbi The cysteine-rich region of T1R3 determines responses to intensely sweet proteins
    Peihua Jiang
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 279:45068-75. 2004
    ..It has been shown that heterologous expression of T1R2 plus T1R3 together yields a receptor responsive to many of the above-mentioned sweet tasting ligands...
  10. ncbi Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste
    Peihua Jiang
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:15238-46. 2005
    The detection of sweet-tasting compounds is mediated in large part by a heterodimeric receptor comprised of T1R2+T1R3. Lactisole, a broad-acting sweet antagonist, suppresses the sweet taste of sugars, protein sweeteners, and artificial ..
  11. pmc Human receptors for sweet and umami taste
    Xiaodong Li
    Senomyx, Inc, 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:4692-6. 2002
    ..Here we show that human T1R2/T1R3 recognizes diverse natural and synthetic sweeteners...
  12. ncbi Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac
    M Max
    Department of Physiology and Biophysics, Mount Sinai School of Medicine of New York University, New York, New York, USA
    Nat Genet 28:58-63. 2001
    ..Mouse Tas1r3 (encoding T1r3) maps to within 20,000 bp of the marker closest to Sac (ref...
  13. pmc Allelic variation of the Tas1r3 taste receptor gene selectively affects taste responses to sweeteners: evidence from 129.B6-Tas1r3 congenic mice
    Masashi Inoue
    Laboratory of Cellular Neurobiology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
    Physiol Genomics 32:82-94. 2007
    The Tas1r3 gene encodes the T1R3 receptor protein, which is involved in sweet taste transduction...
  14. pmc The sweet taste quality is linked to a cluster of taste fibers in primates: lactisole diminishes preference and responses to sweet in S fibers (sweet best) chorda tympani fibers of M. fascicularis monkey
    Yiwen Wang
    Department of Physiology and Pharmacology, Medical School, University of Minnesota Duluth, 1035 University Dr, Duluth, MN 55812, USA
    BMC Physiol 9:1. 2009
    ..In this study we apply a new sweet taste modifier, lactisole, commercially available as a suppressor of the sweetness of sugars on the human tongue, to test our hypothesis that sweet taste is conveyed in S fibers...
  15. ncbi Comparison of L-monosodium glutamate and L-amino acid taste in rats
    E R Delay
    Department of Biology, Marsh Life Science, University of Vermont, Burlington, VT 05405, USA
    Neuroscience 148:266-78. 2007
    T1R2/T1R3 heterodimers are selectively responsive to sweet substances whereas T1R1/T1R3 receptors are selective for umami substances, represented by monosodium glutamate (MSG), and for L-amino acids...
  16. pmc Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2
    Oliver J Mace
    Department of Biology Area 3, University of York, York YO10 5YW, UK
    J Physiol 582:379-92. 2007
    ..occurs within minutes by an increase in apical GLUT2, which correlates with reciprocal regulation of T1R2, T1R3 and alpha-gustducin versus T1R1, transducin and phospholipase C beta2...
  17. doi Antagonism of metabotropic glutamate receptor 4 receptors by (RS)-alpha-cyclopropyl-4-phosphonophenylglycine alters the taste of amino acids in rats
    B K Eschle
    Department of Biology and Vermont Chemical Senses Group, 109 Carrigan Drive, University of Vermont, Burlington, VT 05405, USA
    Neuroscience 163:1292-301. 2009
    T1R1/T1R3, taste-metabotropic glutamate receptor (mGluR) 4 and other taste receptors have been implicated in umami taste perceptionT1R1/T1R3 has also been identified as an L-amino acid receptor...
  18. pmc Stevia and saccharin preferences in rats and mice
    Anthony Sclafani
    Department of Psychology, Brooklyn College, Brooklyn, NY 11210, USA
    Chem Senses 35:433-43. 2010
    ..Similar tests of sweet receptor T1R3 knockout mice and brief-access licking tests with normal mice suggested that the preferences were based on sweet ..
  19. pmc Endocannabinoids selectively enhance sweet taste
    Ryusuke Yoshida
    Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 107:935-9. 2010
    ..Immunohistochemistry shows that CB(1) receptors are expressed in type II taste cells that also express the T1r3 sweet taste receptor component...
  20. doi Nutritional status alters saccharin intake and sweet receptor mRNA expression in rat taste buds
    Ke Chen
    Department of Physiology and Pathophysiology, Xian JiaoTong University School of Medicine, Xian, Shaanxi, PR China
    Brain Res 1325:53-62. 2010
    ..Consistent with the changes in saccharin intake, the gene expression level of the sweet taste receptor T1R3 was significantly decreased in the high-fat diet-induced obese rats compared with the control rats...
  21. pmc Genetically-increased taste cell population with G(alpha)-gustducin-coupled sweet receptors is associated with increase of gurmarin-sensitive taste nerve fibers in mice
    Keiko Yasumatsu
    Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University, Fukuoka 812 8582, Japan
    BMC Neurosci 10:152. 2009
    ..However, the formation of neural pathways from the increased taste cell population to nerve fibers has not yet been examined...
  22. doi The capsaicin receptor participates in artificial sweetener aversion
    Celine E Riera
    Nestle Research Center, Vers chez les Blanc, Perception Physiology, PO Box 44, CH 1000 Lausanne 26, Switzerland
    Biochem Biophys Res Commun 376:653-7. 2008
    ..of the above compounds, preference tests were performed in wild-type mice and mice lacking the TRPV1 channel or the T1R3 receptor, the latter being necessary for the perception of sweet taste...
  23. ncbi Behavioral discrimination between sucrose and other natural sweeteners in mice: implications for the neural coding of T1R ligands
    Cedrick D Dotson
    Department of Psychology and Center for Smell and Taste, University of Florida, Gainesville, Florida 32611 2250, USA
    J Neurosci 27:11242-53. 2007
    ..T1R heteromeric taste receptors mediate signal transduction of sugars (the canonical "sweet" taste receptor, T1R2 + T1R3) and L-amino acids (the T1R1 + T1R3 receptor)...
  24. pmc An energy supply network of nutrient absorption coordinated by calcium and T1R taste receptors in rat small intestine
    Oliver J Mace
    Department of Biology Area 3, The University of York, Heslington, York YO10 5YW, UK
    J Physiol 587:195-210. 2009
    ..Trafficking of apical GLUT2 is rapidly up-regulated by glucose and artificial sweeteners, which act through T1R2 + T1R3/alpha-gustducin to activate PLC beta2 and PKC betaII...
  25. doi Receptors and transduction of umami taste stimuli
    Sue C Kinnamon
    Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, USA
    Ann N Y Acad Sci 1170:55-9. 2009
    ..receptors that bind umami stimuli have been identified in taste buds, including the heterodimer T1R1/T1R3, truncated and brain forms of mGluR4 and mGluR1, brain mGluR2, and brain mGluR3...
  26. pmc Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion
    Yuko Nakagawa
    Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    PLoS ONE 4:e5106. 2009
    ..Sweet taste receptor is expressed in the taste buds and enteroendocrine cells acting as a sugar sensor. We investigated the expression and function of the sweet taste receptor in MIN6 cells and mouse islets...
  27. pmc Involvement of T1R3 in calcium-magnesium taste
    Michael G Tordoff
    Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104 3308, USA
    Physiol Genomics 34:338-48. 2008
    ..The taste receptor gene, Tas1r3, was implicated by studies with 129.B6ByJ-Tas1r3 congenic and Tas1r3 knockout mice...
  28. ncbi Sugar sensing by enterocytes combines polarity, membrane bound detectors and sugar metabolism
    Maude Le Gall
    INSERM, UMR S 872, Centre de Recherche des Cordeliers, Paris, France
    J Cell Physiol 213:834-43. 2007
    ..Finally, we located the sweet-taste receptors T1R3 and T1R2 in plasma membranes, and we measured their cognate G alpha Gustducin mRNA levels...
  29. ncbi Initial licking responses of mice to sweeteners: effects of tas1r3 polymorphisms
    John I Glendinning
    Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA
    Chem Senses 30:601-14. 2005
    ..First, transgenic mice lacking the gene for T1R3, Tas1r3, show dramatically reduced lick responsiveness to most sweeteners...
  30. pmc Valine 738 and lysine 735 in the fifth transmembrane domain of rTas1r3 mediate insensitivity towards lactisole of the rat sweet taste receptor
    Marcel Winnig
    German Institute of Human Nutrition Potsdam Rehbruecke, Department of Molecular Genetics, Arthur Scheunert Allee 114 116, 14558 Nuthetal, Germany
    BMC Neurosci 6:22. 2005
    The sweet taste inhibitor lactisole acts on the human sweet taste receptor heteromer TAS1R2-TAS1R3 but not on its rodent counterpart...
  31. ncbi Temporal changes in NCAM immunoreactivity during taste cell differentiation and cell lineage relationships in taste buds
    Hirohito Miura
    National Food Research Institute, 2 1 12 Kannondai, Ibaraki 305 8642, Japan
    Chem Senses 30:367-75. 2005
    ..expression of NCAM was examined in Mash1-expressing taste cells of adult mice in comparison with gustducin- and T1r3-expressing cells, using a combination of NCAM immunohistochemistry and in situ hybridization...
  32. pmc Taste receptors for umami: the case for multiple receptors
    Nirupa Chaudhari
    Department of Physiology and Biophysics, University of Miami, FL 33136, USA
    Am J Clin Nutr 90:738S-742S. 2009
    ..Loss of the Tas1r1 or Tas1R3 genes is reported to depress but not eliminate neural and behavioral responses to umami...
  33. pmc Contribution of the T1r3 taste receptor to the response properties of central gustatory neurons
    Christian H Lemon
    Department of Anatomy, University of Tennessee Health Science Center, Memphis, Tennessee, USA
    J Neurophysiol 101:2459-71. 2009
    b>T1r3 is a critical subunit of T1r sweet taste receptors. Here we studied how the absence of T1r3 impacts responses to sweet stimuli by taste neurons in the nucleus tractus solitarius (NTS) of the mouse...
  34. ncbi Multiple receptor systems for glutamate detection in the taste organ
    Toshiaki Yasuo
    Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biol Pharm Bull 31:1833-7. 2008
    ..To date, several GPCRs for umami compounds have been identified in taste cells, including the heterodimer T1R1/T1R3, and truncated type 1 and 4 metabotropic glutamate receptors missing most of the N-terminal extracellular domain (..
  35. pmc Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue
    Marco Tizzano
    Rocky Mountain Taste and Smell Center, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
    BMC Neurosci 9:110. 2008
    ..Galphagus (gustducin) couples to the T2R (bitter) receptors, which Galpha-subunit couples to the sweet (T1R2 + T1R3) receptor is presently not known...
  36. doi Expression of taste molecules in the upper gastrointestinal tract in humans with and without type 2 diabetes
    R L Young
    Discipline of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, Australia
    Gut 58:337-46. 2009
    ..In the tongue, sugars are detected via taste T1R2 and T1R3 receptors and signalled via the taste G-protein alpha-gustducin (G alpha(gust)) and the transient receptor ..
  37. pmc Variation in umami perception and in candidate genes for the umami receptor in mice and humans
    Noriatsu Shigemura
    Kyushu University, Fukuoka, Japan
    Am J Clin Nutr 90:764S-769S. 2009
    ..consists of the T1R1 (taste receptor type 1, member 1, Tas1r1) and the T1R3 (taste receptor type 1, member 3, Tas1r3) proteins, functions as an umami taste receptor for rodents and humans...
  38. ncbi Perception of sweet taste is important for voluntary alcohol consumption in mice
    Y A Blednov
    Waggoner Center for Alcohol and Addiction Research, 1 University Station A4800, Austin, TX 78712 0159, USA
    Genes Brain Behav 7:1-13. 2008
    ..mice, each lacking a gene expressed in taste buds and critical to taste transduction: alpha-gustducin (Gnat3), Tas1r3 or Trpm5...
  39. ncbi Taste-signaling proteins are coexpressed in solitary intestinal epithelial cells
    Carole Bezençon
    Nestle Research Center, Vers chez les Blanc, Lausanne, Switzerland
    Chem Senses 32:41-9. 2007
    ..The PCR data showed that T1r1, T1r2, T1r3, alpha-gustducin, phospholipase Cbeta2 (PLCbeta2), and Trpm5 are expressed in the stomach, small intestine, and ..
  40. pmc Transsynaptic transport of wheat germ agglutinin expressed in a subset of type II taste cells of transgenic mice
    Sami Damak
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1677, New York, New York, 10029, USA
    BMC Neurosci 9:96. 2008
    ..To determine how WGA produced in T1r3-expressing taste cells is transported into gustatory neurons, transgenic mice expressing WGA-IRES-GFP driven by the ..
  41. pmc Differential effects of sucrose and fructose on dietary obesity in four mouse strains
    John I Glendinning
    Department of Biological Sciences, Barnard College, Columbia University, New York, NY 10027, United States
    Physiol Behav 101:331-43. 2010
    We examined sugar-induced obesity in mouse strains polymorphic for Tas1r3, a gene that codes for the T1R3 sugar taste receptor...
  42. doi Multiple receptors underlie glutamate taste responses in mice
    Keiko Yasumatsu
    Kyushu University, Higashi ku, Fukuoka, Japan
    Am J Clin Nutr 90:747S-752S. 2009
    ..studies have identified several candidate receptors for umami in taste cells, such as the heterodimer T1R1/T1R3 and brain-expressed and taste-expressed type 1 and 4 metabotropic glutamate receptors (brain-mGluR1, brain-mGluR4, ..
  43. pmc Role of olfaction in the conditioned sucrose preference of sweet-ageusic T1R3 knockout mice
    Steven Zukerman
    Department of Psychology, Brooklyn College of CUNY, Brooklyn, NY 11210, USA
    Chem Senses 34:685-94. 2009
    Prior work has shown that sweet taste-deficient T1R3 knockout (KO) mice developed significant sucrose preferences when given long-term sugar versus water tests...
  44. doi Generation and characterization of T1R2-LacZ knock-in mouse
    Ken Iwatsuki
    Institute for Innovation, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki 210 8681, Japan
    Biochem Biophys Res Commun 402:495-9. 2010
    ..has been identified as a sole sweet taste receptor in mammals that mediates sweet signals upon dimerization with T1R3. However, because of limited availability of reliable antibodies and low expression level of G protein-coupled ..
  45. ncbi Expression of metabotropic glutamate receptor group I in rat gustatory papillae
    Takashi Toyono
    Department of Oral Anatomy and Neurobiology, Kyushu Dental College, Kokurakita ku, 803 8580, Kitakyushu, Japan
    Cell Tissue Res 313:29-35. 2003
    Taste-metabotropic glutamate receptor 4 (taste-mGluR4) and the heteromers of T1R1 and T1R3 are candidate receptors involved in the sense of umami (monosodium glutamate) taste...
  46. doi The neuropeptides CCK and NPY and the changing view of cell-to-cell communication in the taste bud
    Scott Herness
    College of Dentistry, The Ohio State University, Columbus, OH 43210, USA
    Physiol Behav 97:581-91. 2009
    ..Preliminary evidence suggests the NPY-1 receptor expressing cell co-expresses T1R3, a member of the T1R family of G-protein coupled receptors thought to be important in detection of sweet and umami ..
  47. pmc Sweet taste signaling functions as a hypothalamic glucose sensor
    Xueying Ren
    The John B Pierce Laboratory New Haven, CT, USA
    Front Integr Neurosci 3:12. 2009
    ..Here we describe for the first time the expression of the taste receptor genes Tas1r1, Tas1r2 and Tas1r3, and their associated G-protein genes, in the mammalian brain...
  48. ncbi Sucrose and monosodium glutamate taste thresholds and discrimination ability of T1R3 knockout mice
    E R Delay
    Department of Psychology, Regis University, Denver, CO, USA
    Chem Senses 31:351-7. 2006
    ..studies have identified heterodimers of the T1R family as receptors for detecting the tastes of sweet (T1R2 + T1R3) and umami (T1R1 + T1R3)...
  49. doi Behavioral studies of umami: tales told by mice and rats
    Eugene R Delay
    Department of Biology and Vermont Chemical Senses Group, University of Vermont, Burlington, Vermont 05406, USA
    Ann N Y Acad Sci 1170:41-5. 2009
    ..When the G protein-coupled receptor T1R1+T1R3 was discovered, it was believed to be the umami receptor and a more broadly tuned L-amino acid receptor...
  50. doi Sensory attributes of complex tasting divalent salts are mediated by TRPM5 and TRPV1 channels
    Celine E Riera
    Nestle Research Center, Vers chez les Blanc, CH 1000 Lausanne, Switzerland
    J Neurosci 29:2654-62. 2009
    ..preference tests (two bottles, brief access) were performed in wild-type (WT) mice and in mice lacking (1) the T1R3 receptor, (2) TRPV1, the capsaicin receptor, or (3) the TRPM5 channel, the latter being necessary for the ..
  51. doi Genetic tracing of the gustatory neural pathway originating from T1R3-expressing sweet/umami taste receptor cells
    Ichiro Matsumoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Ann N Y Acad Sci 1170:46-50. 2009
    ..WGA), was faithfully and robustly expressed in sweet/umami taste receptor cells (TRCs) under the control of mouse T1R3 gene promoter/enhancer...
  52. doi Molecular pharmacology of promiscuous seven transmembrane receptors sensing organic nutrients
    Petrine Wellendorph
    UNIK centre for life style diseases, Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark
    Mol Pharmacol 76:453-65. 2009
    ..receptor, the G protein-coupled receptor family C, group 6, subtype A (GPRC6A), and the taste1 receptor T1R1/T1R3, which are sensing L-alpha-amino acids, the carbohydrate-sensing T1R2/T1R3 receptor, the proteolytic degradation ..
  53. doi Flavor preferences conditioned by oral monosodium glutamate in mice
    Karen Ackroff
    Department of Psychology, Brooklyn College of CUNY, 2900 Bedford Avenue, Brooklyn, NY 11210, USA
    Chem Senses 38:745-58. 2013
    ..Similar to the parent B6 strain, mice missing the T1r3 element of an umami receptor or the downstream signaling component Trpm5 learned to prefer the CS+ flavor and ..
  54. doi Genetics of food preferences: a first view from silk road populations
    Nicola Pirastu
    Inst for Maternal and Child Health IRCCS Burlo Garofolo, Trieste, Italy
    J Food Sci 77:S413-8. 2012
    ..b>TAS1R3 was changed to TAS1R2.] gene and liking of Vodka (P= 1.6 × 10(-3)), white wine (P= 4...
  55. doi Molecular mechanism of the allosteric enhancement of the umami taste sensation
    Ole G Mouritsen
    MEMPHYS Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark
    FEBS J 279:3112-20. 2012
    The fifth taste quality, umami, arises from binding of glutamate to the umami receptor T1R1/T1R3. The umami taste is enhanced several-fold upon addition of free nucleotides such as guanosine-5'-monophosphate (GMP) to glutamate-containing ..
  56. pmc Expression and secretion of TNF-α in mouse taste buds: a novel function of a specific subset of type II taste cells
    Pu Feng
    Monell Chemical Senses Center, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 7:e43140. 2012
    ..markers, the TNF-α-producing cells are predominantly type II taste cells expressing the taste receptor T1R3. These cells can rapidly increase TNF-α production and secretion upon inflammatory challenges, both in vivo and in ..
  57. pmc Sensing of amino acids by the gut-expressed taste receptor T1R1-T1R3 stimulates CCK secretion
    Kristian Daly
    Epithelial Function and Development Group, Department of Functional and Comparative Genomics, University of Liverpool, Liverpool, UK
    Am J Physiol Gastrointest Liver Physiol 304:G271-82. 2013
    ..of a broad spectrum of L-amino acids is accomplished by the heteromeric amino acid (umami) taste receptor (T1R1-T1R3). T1R1 and T1R3 subunits are also expressed in the intestine...
  58. doi Dietary lipids and sweeteners regulate glucagon-like peptide-2 secretion
    Shingo Sato
    Department of Internal Medicine, National Defense Medical College, Saitama, Japan
    Am J Physiol Gastrointest Liver Physiol 304:G708-14. 2013
    ..GLP-2 secretion induced by sweetening agents was inhibited by lactisole, a sweetness-antagonizing inhibitor of T1R3. In contrast, lactisole was unable to inhibit GLP-2 secretion induced by αLA alone...
  59. pmc The G protein-coupled taste receptor T1R1/T1R3 regulates mTORC1 and autophagy
    Eric M Wauson
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Dallas, TX 75390 9041, USA
    Mol Cell 47:851-62. 2012
    ..We identify the G protein-coupled receptor (GPCR) T1R1/T1R3 as a direct sensor of the fed state and amino acid availability...
  60. doi Angiotensin II modulates salty and sweet taste sensitivities
    Noriatsu Shigemura
    Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Neurosci 33:6267-77. 2013
    ..were coexpressed with αENaC (epithelial sodium channel α-subunit, an amiloride-sensitive salt taste receptor) or T1r3 (a sweet taste receptor component). These results suggest that the taste organ is a peripheral target of AngII...
  61. pmc Expression, regulation and putative nutrient-sensing function of taste GPCRs in the heart
    Simon R Foster
    University of Queensland, School of Biomedical Sciences, Brisbane, Australia
    PLoS ONE 8:e64579. 2013
    ..and human heart tissues that revealed discrete subsets of type 2 taste receptors (TAS2/Tas2) as well as Tas1r1 and Tas1r3 (comprising the umami receptor) are expressed...
  62. pmc Umami responses in mouse taste cells indicate more than one receptor
    Yutaka Maruyama
    Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    J Neurosci 26:2227-34. 2006
    ..metabotropic glutamate receptor 4, truncated metabotropic glutamate receptor 1, or taste receptor 1 (T1R1) and T1R3 dimers], taken alone, did not appear to explain the taste responses observed in mouse taste cells...
  63. ncbi The heterodimeric sweet taste receptor has multiple potential ligand binding sites
    Meng Cui
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1218, New York, NY 10029, USA
    Curr Pharm Des 12:4591-600. 2006
    The sweet taste receptor is a heterodimer of two G protein coupled receptors, T1R2 and T1R3. This discovery has increased our understanding at the molecular level of the mechanisms underlying sweet taste...
  64. doi Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor
    Elodie Maitrepierre
    Centre des Sciences du Gout et de l Alimentation, UMR6265 CNRS, UMR1324 INRA, Universite de Bourgogne, F 21000 Dijon, France
    Protein Expr Purif 83:75-83. 2012
    The sweet taste receptor is a heterodimeric receptor composed of the T1R2 and T1R3 subunits, while T1R1 and T1R3 assemble to form the umami taste receptor. T1R receptors belong to the family of class C G-protein coupled receptors (GPCRs)...
  65. doi Umami taste receptor functions as an amino acid sensor via Gαs subunit in N1E-115 neuroblastoma cells
    Yoshikage Muroi
    Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080 8555, Japan
    J Cell Biochem 113:1654-62. 2012
    ..we examined the function of umami taste receptor, which has a dimeric protein structure composed of Tas1r1 and Tas1r3, as amino acid sensor in the cells...
  66. pmc Age-related changes in mouse taste bud morphology, hormone expression, and taste responsivity
    Yu Kyong Shin
    Laboratory of Clinical Investigation, Diabetes Section, National Institute on Aging, Baltimore, MD 21224, USA
    J Gerontol A Biol Sci Med Sci 67:336-44. 2012
    ..The number of taste cells expressing the sweet taste receptor subunit, T1R3, and the sweet taste modulating hormone, glucagon-like peptide-1, were reduced in the 18-month-old mice...
  67. pmc Umami taste in mice uses multiple receptors and transduction pathways
    Keiko Yasumatsu
    Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Physiol 590:1155-70. 2012
    ..Proposed umami receptors include heteromers of taste receptor type 1, members 1 and 3 (T1R1+T1R3), and metabotropic glutamate receptors 1 and 4 (mGluR1 and mGluR4)...
  68. pmc Glucose transporters and ATP-gated K+ (KATP) metabolic sensors are present in type 1 taste receptor 3 (T1r3)-expressing taste cells
    Karen K Yee
    Monell Chemical Senses Center, Philadelphia, PA 19104 3308, USA
    Proc Natl Acad Sci U S A 108:5431-6. 2011
    Although the heteromeric combination of type 1 taste receptors 2 and 3 (T1r2 + T1r3) is well established as the major receptor for sugars and noncaloric sweeteners, there is also evidence of T1r-independent sweet taste in mice, ..
  69. doi Functional crosstalk between GPCRs: with or without oligomerization
    Laurent Prezeau
    Department of Molecular Pharmacology, CNRS, UMR 5203, Institut de Genomique Fonctionnelle, Montpellier, France
    Curr Opin Pharmacol 10:6-13. 2010
    ..C GPCRs are well-known dimeric receptors, either homodimeric (mGlu or CaSR) or heterodimeric (GABA(B) or taste T1R1/T1R3 and T1R2/T1R3) receptors...
  70. doi Chronic restraint stress in rats suppresses sweet and umami taste responses and lingual expression of T1R3 mRNA
    Atsuhiro Okamoto
    Division of Integrative Physiology, Department of Functional, Morphological and Regulatory Science, Tottori University Faculty of Medicine, Yonago, Tottori 683, Japan
    Neurosci Lett 486:211-4. 2010
    ..In addition, the mRNA expression for T1R3, the common G-protein-coupled receptor (GPCR) for sweet and umami tastes, was studied quantitatively by RT-PCR ..
  71. pmc Glucagon signaling modulates sweet taste responsiveness
    Amanda E T Elson
    Department of Anatomy and Neurobiology, University of Maryland School of Medicine, 20 S Penn St, Rm S251, Baltimore, MD 21201, USA
    FASEB J 24:3960-9. 2010
    ..Most of these cells also express the T1R3 taste receptor implicated in sweet and/or umami taste...
  72. pmc Strain differences in the neural, behavioral, and molecular correlates of sweet and salty taste in naive, ethanol- and sucrose-exposed P and NP rats
    Jamison Coleman
    Dept of Physiology and Biophysics, Virginia Commonwealth Univ, Richmond, VA 23219, USA
    J Neurophysiol 106:2606-21. 2011
    ..NaCl; chorda tympani (CT) nerve responses to sweet and salty stimuli; and gene expression in the anterior tongue of T1R3 and TRPV1/TRPV1t...
  73. pmc Sodium/glucose cotransporter-1, sweet receptor, and disaccharidase expression in the intestine of the domestic dog and cat: two species of different dietary habit
    D J Batchelor
    Department of Veterinary Preclinical Sciences, University of Liverpool, UK
    Am J Physiol Regul Integr Comp Physiol 300:R67-75. 2011
    ..the expression of the intestinal brush border membrane sodium/glucose cotransporter, SGLT1, sweet receptor, T1R2/T1R3, and disaccharidases in these species adapted to contrasting diets...
  74. doi The emerging role of promiscuous 7TM receptors as chemosensors for food intake
    Petrine Wellendorph
    Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark
    Vitam Horm 84:151-84. 2010
    ..receptor (CaR), the G protein-coupled receptor family C, group 6, subtype A (GPRC6A), and the taste1 receptor T1R1/T1R3-sensing L-α-amino acids; the carbohydrate-sensing T1R2/T1R3 receptor; the proteolytic degradation product sensor ..
  75. doi Nutrient sensing in the gut: interactions between chemosensory cells, visceral afferents and the secretion of satiation peptides
    Robert E Steinert
    Clinical Research Center, Department of Biomedicine and Division of Gastroenterology, University Hospital Basel, Basel, Switzerland
    Physiol Behav 105:62-70. 2011
    ..G-protein coupled receptors (GPCRs) have been identified in EEC including the sweet-taste responsive T1R2/T1R3 heterodimer or GPR120, responsive to free fatty acids (FFAs)...
  76. pmc The functional role of the T1R family of receptors in sweet taste and feeding
    Yada Treesukosol
    Department of Psychology and Program in Neuroscience, Florida State University, 1107 West Call Street, P O Box 3064301, Tallahassee FL 32306, USA
    Physiol Behav 105:14-26. 2011
    ..are three proteins in the family, T1R1, T1R2, and T1R3, encoded by their respective genes, Tas1r1, Tas1r2, and Tas1r3. T1R2 combines with T1R3 to form a heterodimer that binds with sugars and other sweeteners...
  77. doi Dietary glutamate signal evokes gastric juice excretion in dogs
    Raisa Khropycheva
    Laboratory of Physiology of Digestion, Pavlov Institute of Physiology of the Russian Academy of Sciences, St Petersburg, Russia
    Digestion 83:7-12. 2011
    Dietary-free L-glutamate (Glu) in the stomach interacts with specific Glu receptors (T1R1/T1R3 and mGluR1-8) expressed on surface epithelial and gastric gland cells...
  78. doi Binding of glutamate to the umami receptor
    J J Lopez Cascales
    Universidad Politecnica de Cartagena, Bioinformatic and Macromolecules Group, Aulario II, Campus de Alfonso XIII, 30203 Cartagena, Murcia, Spain
    Biophys Chem 152:139-44. 2010
    The umami taste receptor is a heterodimer composed of two members of the T1R taste receptor family: T1R1 and T1R3. It detects glutamate in humans, and is a more general amino acid detector in other species...
  79. pmc Gut T1R3 sweet taste receptors do not mediate sucrose-conditioned flavor preferences in mice
    Anthony Sclafani
    Dept of Psychology, Brooklyn College of CUNY, 2900 Bedford Ave, Brooklyn, NY 11210, USA
    Am J Physiol Regul Integr Comp Physiol 299:R1643-50. 2010
    Most mammals prefer the sweet taste of sugars, which is mediated by the heterodimeric T1R2+T1R3 taste receptor. Sugar appetite is also enhanced by the post-oral reinforcing actions of the nutrient in the gut...
  80. pmc Glucose transporters are expressed in taste receptor cells
    Flavia Merigo
    Department of Neurological, Neuropsychological, Morphological and Movement Sciences, Human Anatomy and Histology Section, School of Medicine, University of Verona, Verona, Italy
    J Anat 219:243-52. 2011
    ..induced by the sensing of sugar of the enteroendocrine cells through activation of sweet taste receptors (T1R2 and T1R3) and their associated elements of G-protein-linked signaling pathways (e.g...
  81. doi A novel sweet taste cell-based sensor
    Teng Hao Wang
    Sensory Science Laboratory, Food Safety Key Lab of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310035, PR China
    Biosens Bioelectron 26:929-34. 2010
    ..The human colorectal carcinoma NCI-H716 cell lines, which express α-gustducin and sweet taste receptor T1R1/T1R3, are cultured on the carbon screen-printed electrode with the pre-coated poly-L-ornithine and lamimin for adhesion ..
  82. doi Nutrient sensing receptors in gastric endocrine cells
    Désirée Haid
    Institute of Physiology, University of Hohenheim, Garbenstrasse 30, 70599, Stuttgart, Germany
    J Mol Histol 42:355-64. 2011
    ..in antral gastric mucosa, we obtained evidence for expression of the gustatory amino acid receptor subunit T1R3 and the calcium-sensing receptor CaSR...
  83. doi Introduction of a negative charge at Arg82 in thaumatin abolished responses to human T1R2-T1R3 sweet receptors
    Keisuke Ohta
    Department of Natural Resources, Graduate School of Global and Environmental Studies, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 413:41-5. 2011
    ..These results indicate that Arg82 plays a central role in determining the sweetness of thaumatin. A strict spatial charge location at residue 82 appears to be required for interaction with sweet receptors...
  84. pmc Behavioral evidence for a glucose polymer taste receptor that is independent of the T1R2+3 heterodimer in a mouse model
    Yada Treesukosol
    Department of Psychology, Florida State University, Tallahassee, Florida 32306 4301, USA
    J Neurosci 31:13527-34. 2011
    Although it is clear that the heterodimer formed by the T1R2 and T1R3 proteins serves as the primary taste receptor for sweeteners, there is growing evidence that responses to glucose polymers may be mediated by a different taste receptor...
  85. doi Expression and distribution of the sweet taste receptor isoforms T1R2 and T1R3 in human and rat bladders
    Ruth A Elliott
    Department of Cancer Studies and Molecular Medicine, Reproductive Sciences Section, University of Leicester, Leicester, United Kingdom
    J Urol 186:2455-62. 2011
    ..Artificial sweeteners augment bladder contraction. We hypothesized that artificial sweeteners activate sweet taste receptors in the bladder. Thus, we investigated the expression of sweet taste receptors in human and rat bladders...
  86. doi Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners
    Andrew W Moran
    Epithelial Function and Development Group, Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool, UK
    Br J Nutr 104:637-46. 2010
    ..Artificial sweeteners are known to act on the intestinal sweet taste receptor T1R2/T1R3 and its partner G-protein, gustducin, to activate pathways leading to SGLT1 up-regulation...
  87. doi Multiple receptor systems for umami taste in mice
    Ryusuke Yoshida
    Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan
    Ann N Y Acad Sci 1170:51-4. 2009
    Recent molecular studies proposed that the T1r1/T1r3 heterodimer, mGluR1 and mGluR4 might function as umami taste receptors in mice. However, the roles of each of these receptors in umami taste are not yet clear...
  88. ncbi Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor
    Piero Andrea Temussi
    Computational Biology Unit, EMBL, Heidelberg, Germany
    FEBS Lett 526:1-4. 2002
    Sweet tasting proteins interact with the same receptor that binds small molecular weight sweeteners, the T1R2-T1R3 G-protein coupled receptor, but the key groups on the protein surface responsible for the biological activity have not yet ..
  89. pmc Monellin (MNEI) at 1.15 A resolution
    J R Hobbs
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, Manchester M60 1QD, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:162-7. 2007
    ..Four stably bound negative ions were also located, providing new insight into potential electrostatic interactions of MNEI with the largely negatively charged surface of the sweet taste receptor T1R2-T1R3.
  90. pmc Taste-evoked responses to sweeteners in the nucleus of the solitary tract differ between C57BL/6ByJ and 129P3/J mice
    Stuart A McCaughey
    Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104, USA
    J Neurosci 27:35-45. 2007
    C57BL/6ByJ (B6) and 129P3/J (129) mice have different alleles of Tas1r3, which is thought to influence gustatory transduction of sweeteners, but studies have provided conflicting results regarding differences in sweetness perception ..
  91. ncbi Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues
    S Kiuchi
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
    Cytogenet Genome Res 115:51-61. 2006
    Taste receptor family 1 member 3, TAS1R3, is shown to be involved in sweet and umami tastes in mouse, and the nucleotide sequence of the gene has been reported in rat, gorilla, and human...
  92. pmc Oral, post-oral and genetic interactions in sweet appetite
    Anthony Sclafani
    Department of Psychology, Brooklyn College and the Graduate School, The City University of New York, Brooklyn, NY 11210, USA
    Physiol Behav 89:525-30. 2006
    Inbred mouse strains differ in their preferences for sweeteners, due in part to variations in their T1R3 sweet taste receptor...
  93. ncbi Crystal structure of neoculin: insights into its sweetness and taste-modifying activity
    Akiko Shimizu-Ibuka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Japan
    J Mol Biol 359:148-58. 2006
    ..on these simulations and the generation of a docking model between neoculin and the sweet-taste receptor, T1R2-T1R3, we propose the hypothesis that neoculin is in dynamic equilibrium between open and closed states, and that the ..
  94. ncbi The history of sweet taste: not exactly a piece of cake
    Pierandrea Temussi
    Universita di Napoli Federico II, Via Cinthia 45, Naples 80126, Italy
    J Mol Recognit 19:188-99. 2006
    ..The analysis of the homology models of all possible dimers allowed by combinations of the human T1R2 and T1R3 sequences of the sweet receptor and the closed (A) and open (B) conformations of the mGluR1 glutamate receptor ..
  95. pmc Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25
    Tod R Clapp
    Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    BMC Biol 4:7. 2006
    ..fluorescent protein (GFP) from two taste-specific promoters to examine Ca2+ signaling in subsets of Type II cells: T1R3-GFP mice were used to identify sweet- and umami-sensitive taste cells, while TRPM5-GFP mice were used to identify ..
  96. ncbi Characterization and long-term maintenance of rat taste cells in culture
    Hakan Ozdener
    Monell Chemical Senses Center, Philadelphia, PA 19104 3308, USA
    Chem Senses 31:279-90. 2006
    ..Gustducin, phospholipase C-beta2 (PLC-beta2), T1R3, and T2R5 mRNA were detected in the cultured cells by reverse transcriptase-polymerase chain reaction...
  97. ncbi Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3
    Peihua Jiang
    Department of Neuroscience and Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Biol Chem 280:34296-305. 2005
    ..human and mouse sweet receptor subunits, we determined that responsiveness to cyclamate requires the human form of T1R3. Using chimeras, we determined that it is the transmembrane domain of hT1R3 that is required for the sweet receptor ..
  98. ncbi RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells
    Lars von Buchholtz
    Institute of Physiology, University of Hohenheim, Garbenstr 30, 70599 Stuttgart, Germany
    Eur J Neurosci 19:1535-44. 2004
    ..taste cells with bitter and sweet transduction components including alpha-gustducin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors...
  99. pmc Allelic variation of the Tas1r3 taste receptor gene selectively affects behavioral and neural taste responses to sweeteners in the F2 hybrids between C57BL/6ByJ and 129P3/J mice
    Masashi Inoue
    Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
    J Neurosci 24:2296-303. 2004
    Recent studies have shown that the T1R3 receptor protein encoded by the Tas1r3 gene is involved in transduction of sweet taste...
  100. pmc Polymorphisms in the taste receptor gene (Tas1r3) region are associated with saccharin preference in 30 mouse strains
    D R Reed
    Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104, USA
    J Neurosci 24:938-46. 2004
    The results of recent studies suggest that the mouse Sac (saccharin preference) locus is identical to the Tas1r3 (taste receptor) gene...
  101. ncbi The receptors for mammalian sweet and umami taste
    Grace Q Zhao
    Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, CA 92093, USA
    Cell 115:255-66. 2003
    ..We now report the behavioral and physiological characterization of T1R1, T1R2, and T1R3 knockout mice...

Research Grants76

  1. Mechanisms of sweet transduction in mammalian taste buds
    Nirupa Chaudhari; Fiscal Year: 2007
    ..In recent years, taste research has focused on the identity of sweet taste receptors, T1R2+T1R3, and their binding sites for sugars and other natural and synthetic sweeteners...
  2. Mechanisms of sweet transduction in mammalian taste buds
    Nirupa Chaudhari; Fiscal Year: 2010
    ..In recent years, taste research has focused on the identity of sweet taste receptors, T1R2+T1R3, and their binding sites for sugars and other natural and synthetic sweeteners...
  3. Role of taste signaling elements in enteroendocrine cells
    Robert F Margolskee; Fiscal Year: 2010
    We have determined that gustducin, T1R3 and several other signaling elements that underlie taste detection/transduction also are expressed in L and K subtypes of enteroendocrine cells...
  4. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2007
    ..identify and characterize a number of taste transduction components, including a G-protein coupled taste receptor T1R3, G protein subunits and a transient receptor potential ion channel TRPM5...
  5. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2010
    ..identify and characterize a number of taste transduction components, including a G-protein coupled taste receptor T1R3, G protein subunits and a transient receptor potential ion channel TRPM5...
  6. VOLTAGE-DEPENDENT ION CHANNELS IN GUSTATION
    Liquan Huang; Fiscal Year: 2009
    ..identify and characterize a number of taste transduction components, including a G-protein coupled taste receptor T1R3, G protein subunits and a transient receptor potential ion channel TRPM5...
  7. Functional characterization of endocrine taste cells
    Robert Margolskee; Fiscal Year: 2009
    ..Further, we hypothesize that taste signaling mediated by gustducin, T1R3, Trpm5 and other taste transduction elements contributes importantly to the endocrine and paracrine responses of ..
  8. Functional characterization of endocrine taste cells
    Robert Margolskee; Fiscal Year: 2009
    ..Further, we hypothesize that taste signaling mediated by gustducin, T1R3, Trpm5 and other taste transduction elements contributes importantly to the endocrine and paracrine responses of ..
  9. Functional characterization of endocrine taste cells
    Robert F Margolskee; Fiscal Year: 2010
    ..Further, we hypothesize that taste signaling mediated by gustducin, T1R3, Trpm5 and other taste transduction elements contributes importantly to the endocrine and paracrine responses of ..
  10. Functional characterization of endocrine taste cells
    Robert F Margolskee; Fiscal Year: 2011
    ..Further, we hypothesize that taste signaling mediated by gustducin, T1R3, Trpm5 and other taste transduction elements contributes importantly to the endocrine and paracrine responses of ..
  11. Comparative Genetics of Sweet Taste in Carnivora
    Peihua Jiang; Fiscal Year: 2011
    ..In Aim 1 we will sequence the genes for all three members of the T1R taste receptor family, Tas1r1, Tas1r2, and Tas1r3, from 15 Carnivora species (meerkat, domestic cat, ferret, genet, lesser and giant panda, South American coati, ..
  12. ELECTROPHYSIOLOGY OF TASTE TRANSDUCTION
    Sue Kinnamon; Fiscal Year: 2007
    ..Several G protein-coupled taste receptors have been identified: T2Rs respond to bitter, T1R2/T1R3 to sweet, and T1R1/T1R3 and taste-mGluR4 to umami stimuli...
  13. ELECTROPHYSIOLOGY OF TASTE TRANSDUCTION
    Sue Kinnamon; Fiscal Year: 2009
    ..Several G protein-coupled taste receptors have been identified: T2Rs respond to bitter, T1R2/T1R3 to sweet, and T1R1/T1R3 and taste-mGluR4 to umami stimuli...
  14. Gustatory neural coding in mice: connecting taste receptors to the brain
    Christian Lemon; Fiscal Year: 2007
    ..Sac influences preference for sweets in mammals and encodes the sweet taste receptor T1r3. In Aim 1, we will measure differences in neural responding to sweet-tasting stimuli between mice ..
  15. Agonist & Antagonist Activity and Binding on the TMD of hT1R3
    Marianna Max; Fiscal Year: 2010
    The human sweet receptor, composed of the monomers T1R2 + T1R3, appears to be the main (and perhaps the only) receptor underlying sweet taste in humans...
  16. Analysis of sweet receptor ligand binding and activation
    Marianna Max; Fiscal Year: 2010
    ..program is to understand the molecular events underlying function of the heterodimeric sweet taste receptor (T1R2+T1R3)...
  17. Analysis of sweet receptor ligand binding and activation
    Robert Margolskee; Fiscal Year: 2006
    ..program is to understand the molecular events underlying function of the heterodimeric sweet taste receptor (T1R2+T1R3)...
  18. Molecular Mechanisms of Sweet Receptor Function
    Marianna Max; Fiscal Year: 2007
    ..The sweet receptor is composed of two type 1 taste receptor monomers (T1R2 plus T1R3), apparently as a heterodimer...
  19. Molecular Mechanisms of Sweet Receptor Function
    Marianna Max; Fiscal Year: 2009
    ..The sweet receptor is composed of two type 1 taste receptor monomers (T1R2 plus T1R3), apparently as a heterodimer...
  20. Monellin activates the human sweet taste receptor
    Emeline Maillet; Fiscal Year: 2007
    The sweet receptor, composed of the monomers T1R2+T1R3, is the principal receptor responsible for sweet taste in mammals. T1R2 and T1R3 are two related G protein-coupled receptors of the T1R type I taste receptor family...
  21. GENETICS OF ALCOHOL RELATED INGESTIVE BEHAVIORS IN MICE
    Alexander Bachmanov; Fiscal Year: 2007
    ..laboratory has recently shown that a quantitative trait locus (QTL) associated with the sweet taste receptor gene, Tas1r3, influences alcohol consumption, suggesting that the taste of alcohol may be an important factor in regulation of ..
  22. Lactisole inhibits the sweet taste receptor
    Peihua Jiang; Fiscal Year: 2004
    The sense of sweet taste is mediated by the sweet taste receptor, T1R2+T1R3, which are two closely related G protein-coupled receptors of T1Rs family. T1R2+T1R3 is a broadly tuned receptor responding to all classes of sweet compounds...
  23. L-amino and acid taste in mice.
    Eugene Delay; Fiscal Year: 2005
    ..Molecular and limited behavioral studies have identified a candidate taste receptor, T1R1/T1R3, which detects umami stimuli, including monosodium glutamate, and many more L-amino acids...
  24. PHYSIOLOGY OF CALCIUM APPETITE
    Michael G Tordoff; Fiscal Year: 2010
    ..implicated in the control of calcium consumption;the calcium-sensing receptor, Casr, and the sweet taste receptor, Tas1r3. This will be done by comparing the receptor activity of a mouse strain that avoids calcium (the C57BL/6J or B6 ..
  25. PHYSIOLOGY OF CALCIUM APPETITE
    MICHAEL TORDOFF; Fiscal Year: 2009
    ..in the control of calcium consumption; the calcium-sensing receptor, Casr, and the sweet taste receptor, Tas1r3. This will be done by comparing the receptor activity of a mouse strain that avoids calcium (the C57BL/6J or B6 ..
  26. PHYSIOLOGY OF CALCIUM APPETITE
    Michael G Tordoff; Fiscal Year: 2010
    ..implicated in the control of calcium consumption;the calcium-sensing receptor, Casr, and the sweet taste receptor, Tas1r3. This will be done by comparing the receptor activity of a mouse strain that avoids calcium (the C57BL/6J or B6 ..
  27. Analysis of sweet receptor ligand binding and activation
    Robert Margolskee; Fiscal Year: 2007
    ..program is to understand the molecular events underlying function of the heterodimeric sweet taste receptor (T1R2+T1R3)...
  28. PSYCHOPHYSICAL EVALUATION OF TASTE FUNCTION
    Alan C Spector; Fiscal Year: 2010
    ..The T1R1 and T1R3 subunits combine to form a heteromer in taste receptor cells that binds with L-amino acids, and the T1R2 and T1R3 ..
  29. PSYCHOPHYSICAL EVALUATION OF TASTE FUNCTION
    ALAN SPECTOR; Fiscal Year: 2007
    ..The T1R1 and T1R3 subunits combine to form a heteromer in taste receptor cells that binds with L-amino acids, and the T1R2 and T1R3 ..
  30. PSYCHOPHYSICAL EVALUATION OF TASTE FUNCTION
    ALAN SPECTOR; Fiscal Year: 2006
    ..The T1R1 and T1R3 subunits combine to form a heteromer in taste receptor cells that binds with L-amino acids, and the T1R2 and T1R3 ..
  31. The structural basis of L-amino acid taste
    Stephan Vigues; Fiscal Year: 2007
    ..T1R1 and T1R3 function as a heteromeric receptor to detect umami stimuli such as L-amino acids...
  32. Orosensory Contributions to Alcohol Ingestion
    Susan M Brasser; Fiscal Year: 2010
    ..New preliminary behavioral data indicate that genetic deletion of the T1r3 sweet taste receptor in mice produces indifference to alcohol in long-term intake tests at concentrations that are ..
  33. Orosensory Contributions to Alcohol Ingestion
    SUSAN BRASSER; Fiscal Year: 2009
    ..New preliminary behavioral data indicate that genetic deletion of the T1r3 sweet taste receptor in mice produces indifference to alcohol in long-term intake tests at concentrations that are ..
  34. Carbohydrate Appetite Fat Appetite and Obesity
    Anthony Sclafani; Fiscal Year: 2007
    ..The findings may provide practical benefits for current attempts to alter dietary fat and carbohydrate intake and control adiposity in humans. ..
  35. Neuropharmacology of Learned Food Preferences
    Anthony Sclafani; Fiscal Year: 2009
    ..It will advance our understanding of the basic mechanisms involved in the development of food preferences and may provide insights into the clinical treatment of overeating and obesity. ..
  36. The brazzein-sweet taste receptor interaction
    Goran Hellekant; Fiscal Year: 2007
    ..a). To test brazzein mutants for direct binding and activity assay with T1R2/T1R3 sweet receptors; b)...
  37. Marking mouse taste neural circuits by transgenic expre*
    Sami Damak; Fiscal Year: 2002
    ..a red or green fluorescent protein marker (RFP or GFP) under the control of the promoter regions of T1R1, T1R3 and mT2R5, and determine by expression studies whether these promoters are able to target expression of ..