TARBP2

Summary

Gene Symbol: TARBP2
Description: TARBP2, RISC loading complex RNA binding subunit
Alias: LOQS, TRBP, TRBP1, TRBP2, RISC-loading complex subunit TARBP2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, trans-activation responsive RNA-binding protein
Species: human
Products:     TARBP2

Top Publications

  1. Duarte M, Graham K, Daher A, Battisti P, Bannwarth S, Segeral E, et al. Characterization of TRBP1 and TRBP2. Stable stem-loop structure at the 5' end of TRBP2 mRNA resembles HIV-1 TAR and is not found in its processed pseudogene. J Biomed Sci. 2000;7:494-506 pubmed
    TRBP1 and TRBP2 cDNAs have been isolated based on the ability of the protein that they encode to bind HIV-1 TAR RNA. The two cDNAs have different 5' end-termini resulting in 21 additional amino acids for TRBP2 protein compared to TRBP1...
  2. Cosentino G, Venkatesan S, Serluca F, Green S, Mathews M, Sonenberg N. Double-stranded-RNA-dependent protein kinase and TAR RNA-binding protein form homo- and heterodimers in vivo. Proc Natl Acad Sci U S A. 1995;92:9445-9 pubmed
    ..The mammalian dsRNA-binding protein TRBP, originally identified on the basis of its ability to bind the transactivation region (TAR) of human ..
  3. Gatignol A, Buckler White A, Berkhout B, Jeang K. Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR. Science. 1991;251:1597-600 pubmed
    ..A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.
  4. Paroo Z, Ye X, Chen S, Liu Q. Phosphorylation of the human microRNA-generating complex mediates MAPK/Erk signaling. Cell. 2009;139:112-22 pubmed publisher
    ..We determined that the human miRNA-generating complex is comprised of Dicer and phospho-TRBP isoforms. Phosphorylation of TRBP is mediated by the mitogen-activated protein kinase (MAPK) Erk...
  5. Singh M, Castillo D, Patel C, Patel R. Stress-induced phosphorylation of PACT reduces its interaction with TRBP and leads to PKR activation. Biochemistry. 2011;50:4550-60 pubmed publisher
    ..In addition to positive regulation by PACT, PKR activity in cells is also negatively regulated by TRBP. In this study, we demonstrate for the first time that stress-induced phosphorylation at serine 287 significantly ..
  6. Daher A, Longuet M, Dorin D, Bois F, Segeral E, Bannwarth S, et al. Two dimerization domains in the trans-activation response RNA-binding protein (TRBP) individually reverse the protein kinase R inhibition of HIV-1 long terminal repeat expression. J Biol Chem. 2001;276:33899-905 pubmed
    Trans-activation response (TAR) RNA-binding protein (TRBP) is a cellular protein that binds to the human immunodeficiency virus-1 (HIV-1) TAR element RNA...
  7. Bannwarth S, Talakoub L, Letourneur F, Duarte M, Purcell D, Hiscott J, et al. Organization of the human tarbp2 gene reveals two promoters that are repressed in an astrocytic cell line. J Biol Chem. 2001;276:48803-13 pubmed
    ..We have isolated and characterized the complete tarbp2 gene (5493 bp) coding for the two TRBP proteins...
  8. Battisti P, Daher A, Bannwarth S, Voortman J, Peden K, Hiscott J, et al. Additive activity between the trans-activation response RNA-binding protein, TRBP2, and cyclin T1 on HIV type 1 expression and viral production in murine cells. AIDS Res Hum Retroviruses. 2003;19:767-78 pubmed
    ..The trans-activation response (TAR) RNA-binding protein 2 (TRBP2) increases HIV-1 LTR expression through TAR and protein kinase R (PKR) binding, but not through interactions with ..
  9. Parker G, Maity T, Bass B. dsRNA binding properties of RDE-4 and TRBP reflect their distinct roles in RNAi. J Mol Biol. 2008;384:967-79 pubmed publisher
    ..cleavage of long dsRNA to small interfering RNA (siRNA), while human trans-activation response RNA-binding protein (TRBP) functions downstream to pass siRNA to the RNA-induced silencing complex...

More Information

Publications86

  1. Daniels S, Melendez Peña C, Scarborough R, Daher A, Christensen H, El Far M, et al. Characterization of the TRBP domain required for dicer interaction and function in RNA interference. BMC Mol Biol. 2009;10:38 pubmed publisher
    ..Immunofluorescence shows that while full-length TRBPs colocalize with Dicer, TRBPsDeltaC4 do not. tarbp2-/- cells, which do not express TRBP, do not support RNA interference (RNAi) mediated by short hairpin or micro ..
  2. Robb G, Rana T. RNA helicase A interacts with RISC in human cells and functions in RISC loading. Mol Cell. 2007;26:523-37 pubmed
    ..We show that RHA interacts in human cells with siRNA, Ago2, TRBP, and Dicer and functions in the RNAi pathway...
  3. Wang H, Noland C, Siridechadilok B, Taylor D, Ma E, Felderer K, et al. Structural insights into RNA processing by the human RISC-loading complex. Nat Struct Mol Biol. 2009;16:1148-53 pubmed publisher
    ..In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Dicer and the RNA-binding protein TRBP to form a RISC-loading complex (RLC), which is necessary for efficient transfer of nascent siRNAs and miRNAs from ..
  4. Lee Y, Hur I, Park S, Kim Y, Suh M, Kim V. The role of PACT in the RNA silencing pathway. EMBO J. 2006;25:522-32 pubmed
    ..as RNA-induced silencing complex (RISC) by multiple factors including hAgo2, the mRNA-targeting endonuclease, and TRBP (HIV-1 TAR RNA-binding protein), a dsRNA-binding protein that interacts with both Dicer and hAgo2...
  5. Bannwarth S, Lainé S, Daher A, Grandvaux N, Clerzius G, LeBlanc A, et al. Cell-specific regulation of TRBP1 promoter by NF-Y transcription factor in lymphocytes and astrocytes. J Mol Biol. 2006;355:898-910 pubmed
    ..TRBP1 and TRBP2 are double-stranded RNA binding proteins that increase HIV-1 gene expression...
  6. Yamashita S, Nagata T, Kawazoe M, Takemoto C, Kigawa T, Güntert P, et al. Structures of the first and second double-stranded RNA-binding domains of human TAR RNA-binding protein. Protein Sci. 2011;20:118-30 pubmed publisher
    The TAR RNA-binding Protein (TRBP) is a double-stranded RNA (dsRNA)-binding protein, which binds to Dicer and is required for the RNA interference pathway. TRBP consists of three dsRNA-binding domains (dsRBDs)...
  7. Chendrimada T, Gregory R, Kumaraswamy E, Norman J, Cooch N, Nishikura K, et al. TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature. 2005;436:740-4 pubmed
    ..Here we show that TRBP (the human immunodeficiency virus transactivating response RNA-binding protein), which contains three double-..
  8. Lee J, Kim H, Ryu C, Kim J, Choi B, Lim Y, et al. Merlin, a tumor suppressor, interacts with transactivation-responsive RNA-binding protein and inhibits its oncogenic activity. J Biol Chem. 2004;279:30265-73 pubmed
    ..modulators through protein-protein interactions, we identified transactivation-responsive RNA-binding protein (TRBP) as a merlin-binding protein in a yeast two-hybrid screen...
  9. Daviet L, Erard M, Dorin D, Duarte M, Vaquero C, Gatignol A. Analysis of a binding difference between the two dsRNA-binding domains in TRBP reveals the modular function of a KR-helix motif. Eur J Biochem. 2000;267:2419-31 pubmed
    Double-stranded RNA-binding proteins constitute a large family with conserved domains called dsRBDs. One of these, TRBP, a protein that binds HIV-1 TAR RNA, has two dsRBDs (dsRBD1 and dsRBD2), as indicated by computer sequence homology...
  10. Koscianska E, Starega Roslan J, Krzyzosiak W. The role of Dicer protein partners in the processing of microRNA precursors. PLoS ONE. 2011;6:e28548 pubmed publisher
    ..Human Dicer performs this function in cooperation with its protein partners, AGO2, PACT and TRBP. The exact role of these accessory proteins in Dicer activity is still poorly understood...
  11. Gatignol A, Buckler C, Jeang K. Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufen. Mol Cell Biol. 1993;13:2193-202 pubmed
    b>TRBP is a human cellular protein that binds the human immunodeficiency virus type 1 TAR RNA. Here, we show that the intact presence of amino acids 247 to 267 in TRBP correlates with its ability to bind RNA...
  12. Benoit M, Imbert L, Palencia A, Perard J, Ebel C, Boisbouvier J, et al. The RNA-binding region of human TRBP interacts with microRNA precursors through two independent domains. Nucleic Acids Res. 2013;41:4241-52 pubmed publisher
    ..HIV TAR element binding protein (TRBP) is a constituent of the Dicer complex, which augments complex stability and potentially functions in substrate ..
  13. Horikawa Y, Wood C, Yang H, Zhao H, Ye Y, Gu J, et al. Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. Clin Cancer Res. 2008;14:7956-62 pubmed publisher
    ..40 single nucleotide polymorphisms (SNP) from 11 miRNA processing genes (DROSHA, DGCR8, XPO5, RAN, DICER1, TARBP2, AGO1, AGO2, GEMIN3, GEMIN4, HIWI) and 15 miRNA genes in 279 Caucasian patients with renal cell carcinoma and 278 ..
  14. Lee H, Doudna J. TRBP alters human precursor microRNA processing in vitro. RNA. 2012;18:2012-9 pubmed publisher
    ..We show here that the human trans-activation response (TAR) RNA binding protein (TRBP), a known molecular partner of the miRNA processing enzyme Dicer, changes the rates of pre-miRNA cleavage in an RNA-..
  15. Daher A, Laraki G, Singh M, Melendez Peña C, Bannwarth S, Peters A, et al. TRBP control of PACT-induced phosphorylation of protein kinase R is reversed by stress. Mol Cell Biol. 2009;29:254-65 pubmed publisher
    ..PACT-induced PKR phosphorylation was restored in Tarbp2(-/-) murine tail fibroblasts and in HEK293T or HeLa cells when TRBP expression was reduced by RNA interference...
  16. Macrae I, Ma E, Zhou M, Robinson C, Doudna J. In vitro reconstitution of the human RISC-loading complex. Proc Natl Acad Sci U S A. 2008;105:512-7 pubmed publisher
    ..action of a specialized assembly called the RISC-loading complex (RLC), comprising the proteins Ago2, Dicer, and TRBP. Here we show that the human RLC assembles spontaneously in vitro from purified components...
  17. Höck J, Weinmann L, Ender C, Rüdel S, Kremmer E, Raabe M, et al. Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. EMBO Rep. 2007;8:1052-60 pubmed
    ..Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation. ..
  18. Kok K, Ng M, Ching Y, Jin D. Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA. J Biol Chem. 2007;282:17649-57 pubmed
    Mammalian Dicer interacts with double-stranded RNA-binding protein TRBP or PACT to mediate RNA interference and micro-RNA processing. TRBP and PACT are structurally related but exert opposite regulatory activities on PKR...
  19. Christensen H, Daher A, Soye K, Frankel L, Alexander M, Lainé S, et al. Small interfering RNAs against the TAR RNA binding protein, TRBP, a Dicer cofactor, inhibit human immunodeficiency virus type 1 long terminal repeat expression and viral production. J Virol. 2007;81:5121-31 pubmed
    ..complex, in which Dicer, Ago2, and the human immunodeficiency virus type 1 (HIV-1) TAR RNA binding protein (TRBP) are the main components...
  20. Maniataki E, Mourelatos Z. A human, ATP-independent, RISC assembly machine fueled by pre-miRNA. Genes Dev. 2005;19:2979-90 pubmed
    ..the assembly of human RISC by presenting pre-miRNA to immunopurified complexes that contain Ago2, Dicer, and TRBP. Mature miRNAs were produced in an ATP-independent manner and guided specific cleavage of cognate RNA targets in a ..
  21. Haase A, Jaskiewicz L, Zhang H, Lainé S, Sack R, Gatignol A, et al. TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing. EMBO Rep. 2005;6:961-7 pubmed
    ..In this work, we describe the identification of TRBP (human immunodeficiency virus (HIV-1) transactivating response (TAR) RNA-binding protein) as a protein partner of ..
  22. Gatignol A, Duarte M, Daviet L, Chang Y, Jeang K. Sequential steps in Tat trans-activation of HIV-1 mediated through cellular DNA, RNA, and protein binding factors. Gene Expr. 1996;5:217-28 pubmed
    ..We have previously cloned two of them by RNA probe recognition: TRBP and La...
  23. Lau P, Potter C, Carragher B, Macrae I. Structure of the human Dicer-TRBP complex by electron microscopy. Structure. 2009;17:1326-32 pubmed publisher
    ..Here, we present the three-dimensional structure of human Dicer bound to the protein TRBP at approximately 20 A resolution determined by negative-stain electron microscopy (EM) and single-particle analysis...
  24. Xie J, Peng T, Zhu A, He J, Chang Q, Hu X, et al. Multi-residue analysis of veterinary drugs, pesticides and mycotoxins in dairy products by liquid chromatography-tandem mass spectrometry using low-temperature cleanup and solid phase extraction. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1002:19-29 pubmed publisher
    ..The limits of detection (LODs) and limits of quantification (LOQs) were 0.006-0.3μg/kg and 0.02-1.0μg/kg, respectively...
  25. Shen J, Xia W, Khotskaya Y, Huo L, Nakanishi K, Lim S, et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature. 2013;497:383-7 pubmed publisher
    ..These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells and has potential clinical implications. ..
  26. Ma J, Zhao L, Rui Q, Liao Y, Chen Y, Xu J, et al. Application of carbon nanosorbent for PRiME pass-through cleanup of 10 selected local anesthetic drugs in human plasma samples. Anal Chim Acta. 2017;960:72-80 pubmed publisher
    ..The limits of quantification (LOQs) for local anesthetic drugs are in the range of 0.024-0.15 μg/L...
  27. Daniels S, Sinck L, Ward N, Melendez Peña C, Scarborough R, Azar I, et al. HIV-1 RRE RNA acts as an RNA silencing suppressor by competing with TRBP-bound siRNAs. RNA Biol. 2015;12:123-35 pubmed publisher
    ..We demonstrate that RRE binds to TAR-RNA Binding Protein (TRBP), an essential component of the RNA Induced Silencing Complex (RISC)...
  28. Haroune L, Cassoulet R, Lafontaine M, Bélisle M, Garant D, Pelletier F, et al. Liquid chromatography-tandem mass spectrometry determination for multiclass pesticides from insect samples by microwave-assisted solvent extraction followed by a salt-out effect and micro-dispersion purification. Anal Chim Acta. 2015;891:160-70 pubmed publisher
    ..The limits of detection (LODs) and quantification (LOQs) were in the ranges of 0.10-3.00 ng g(-1) and 0.40-7.00 ng g(-1), respectively...
  29. Feng X, Pan L, Wang C, Zhang H. Residue analysis and risk assessment of pyrethrins in open field and greenhouse turnips. Environ Sci Pollut Res Int. 2018;25:877-886 pubmed publisher
    ..1-104%) with limits of quantification (LOQs) of 0.05 mg/kg...
  30. Andrade P, Dantas R, Moura Alves T, Caldas E. Determination of multi-mycotoxins in cereals and of total fumonisins in maize products using isotope labeled internal standard and liquid chromatography/tandem mass spectrometry with positive ionization. J Chromatogr A. 2017;1490:138-147 pubmed publisher
    ..method was based on extraction with acidified acetonitrile and LC-ESI+-MS/MS analysis, with LOQs ranging from 0...
  31. Picó Y, El Sheikh M, Alfarhan A, Barceló D. Target vs non-target analysis to determine pesticide residues in fruits from Saudi Arabia and influence in potential risk associated with exposure. Food Chem Toxicol. 2018;111:53-63 pubmed publisher
    ..Limits of quantification (LOQs) were from 0.5 to 6.3 μg kg-1 for the target method and from 0...
  32. Li J, Zhang X, Lin Z, Huang Z, Rao Y. Efficient determination of six fatty acid ethyl ethers in human whole blood by gas chromatography-mass spectrometry. Alcohol. 2017;62:41-47 pubmed publisher
    ..The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 5-50 ng/mL and 15-200 ng/mL, respectively...
  33. Kakumani P, Rajgokul K, Ponia S, Kaur I, Mahanty S, Medigeshi G, et al. Dengue NS3, an RNAi suppressor, modulates the human miRNA pathways through its interacting partner. Biochem J. 2015;471:89-99 pubmed publisher
    ..with hHSC70 perturbs the formation of RISC (RNA-induced silencing complex)-loading complex (RLC), by displacing TRBP (TAR RNA-binding protein) and possibly impairing the downstream activity of miRNAs...
  34. Dallegrave A, Pizzolato T, Barreto F, Eljarrat E, Barcelo D. Methodology for trace analysis of 17 pyrethroids and chlorpyrifos in foodstuff by gas chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2016;408:7689-7697 pubmed
    ..002 to 6.43 ng g-1 lipid weight (lw), and limits of quantification (LOQs) ranged between 0.006 and 21.4 ng g-1 lw...
  35. Swart C, Gantois F, Petrov P, Entwisle J, Goenaga Infante H, Nousiainen M, et al. Potential reference measurement procedures for PBDE in surface water at levels required by the EU Water Frame Directive. Talanta. 2016;152:251-8 pubmed publisher
    ..Up to now, no reference measurement procedures have been established reaching the limits of quantification (LOQs) and the associated uncertainties as defined in the directives with results traceable to the SI...
  36. Lee W, Park N, Ahn T, Chung B, Hong J. Profiling of a wide range of neurochemicals in human urine by very-high-performance liquid chromatography-tandem mass spectrometry combined with in situ selective derivatization. J Chromatogr A. 2017;1526:47-57 pubmed publisher
    ..The correlation coefficients (R2) of calibration curves were above 0.9961. The ranges of LODs and LOQs were 0.1-3.6ng/mL and 0.3-12.0ng/mL, respectively. The overall precision and accuracy were 0.52-16.74% and 82...
  37. Cao X, Jiang Z, Wang S, Hong S, Li H, Zhang C, et al. Metal-organic framework UiO-66 for rapid dispersive solid phase extraction of neonicotinoid insecticides in water samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1077-1078:92-97 pubmed publisher
    ..Under the optimized conditions, the limits of detection (LODs, S/N = 3) and limits of quantification (LOQs, S/N = 10) for the five insecticides were found to be 0.02-0.4 ng/mL and 0.05-1...
  38. Rjabova J, Viksna A, Zacs D. Development and optimization of gas chromatography coupled to high resolution mass spectrometry based method for the sensitive determination of Dechlorane plus and related norbornene-based flame retardants in food of animal origin. Chemosphere. 2018;191:597-606 pubmed publisher
    ..Ultra-trace level sensitivity was achieved with the instrumental limits of quantification (i-LOQs) varying from ∼0...
  39. Petrarca M, Ccanccapa Cartagena A, Masia A, Godoy H, Pico Y. Comparison of green sample preparation techniques in the analysis of pyrethrins and pyrethroids in baby food by liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2017;1497:28-37 pubmed publisher
    ..and simple and fast execution, provided a lower enrichment factor, consequently, higher limits of quantification (LOQs) were obtained. Some of them too high to meet the strict legislation regarding baby food...
  40. Goyon A, Sciascera L, Clarke A, Guillarme D, Pell R. Extending the limits of size exclusion chromatography: Simultaneous separation of free payloads and related species from antibody drug conjugates and their aggregates. J Chromatogr A. 2018;1539:19-29 pubmed publisher
    ..recoveries (between 82% and 107%) were found to be suitable with both approaches (SEC and SECxRPLC), whereas the LOQs remain similar...
  41. Salihu S, Bakar N. A simple method for chromium speciation analysis in contaminated water using APDC and a pre-heated glass tube followed by HPLC-PDA. Talanta. 2018;181:401-409 pubmed publisher
    ..0029mgL-1 and 0.0014mg/L-1 for Cr(III) Cr(VI) respectively. While the limits of quantitation (LOQs), were 0.0098mgL-1 and 0.0047mgL-1 for Cr(III) and Cr(VI) respectively...
  42. Kim C, Ryu H, Chung E, Kim Y. Determination of 18 veterinary antibiotics in environmental water using high-performance liquid chromatography-q-orbitrap combined with on-line solid-phase extraction. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1084:158-165 pubmed publisher
    ..linearities, precisions, relative recoveries, and method detection limits (MDLs)/limits of quantification (LOQs) of the selected antibiotics, and applied to the analysis of environmental water samples (groundwater, river water, ..
  43. Kotronoulas A, Marcos J, Segura J, Ventura R, Joglar J, Pozo O. Ultra high performance liquid chromatography tandem mass spectrometric detection of glucuronides resistant to enzymatic hydrolysis: Implications to doping control analysis. Anal Chim Acta. 2015;895:35-44 pubmed publisher
    ..The method was found to be linear and with suitable LODs and LOQs for all metabolites...
  44. Zhao J, Li X, Lu X, Zhang Q, Zhang W. [Determination of four polycyclic aromatic hydrocarbons in olive oil by high performance liquid chromatography-fluorescent detection]. Se Pu. 2017;35:748-754 pubmed publisher
    ..4-40 ? g/L with correlation coefficients of 0.9990-0.9999. The limits of quantification (LOQs) ranged from 0.147 to 0.413 ? g/L, and the recoveries of the spiked samples ranged from 95.5% to 103.2%...
  45. Xu F, García Bermejo Ã, Malarvannan G, Gómara B, Neels H, Covaci A. Multi-contaminant analysis of organophosphate and halogenated flame retardants in food matrices using ultrasonication and vacuum assisted extraction, multi-stage cleanup and gas chromatography-mass spectrometry. J Chromatogr A. 2015;1401:33-41 pubmed publisher
    ..Method LOQs ranged between 0.008 and 0.04 ng/g dw for PBDEs, between 0.08 and 0.20 ng/g dw for EFRs, and between 1.4 and 3...
  46. Rachagani S, Macha M, Menning M, Dey P, Pai P, Smith L, et al. Changes in microRNA (miRNA) expression during pancreatic cancer development and progression in a genetically engineered KrasG12D;Pdx1-Cre mouse (KC) model. Oncotarget. 2015;6:40295-309 pubmed publisher
    ..Further, expression of miRNA biosynthetic machinery including Dicer, Exportin-5, TRKRA, and TARBP2 were downregulated, while DGCR8 and Ago2 were upregulated in KC mice...
  47. Chen Y, Cheng X, Mo F, Huang L, Wu Z, Wu Y, et al. Ultra-sensitive speciation analysis of mercury by CE-ICP-MS together with field-amplified sample stacking injection and dispersive solid-phase extraction. Electrophoresis. 2016;37:1055-62 pubmed publisher
    ..we have successfully determined ultra-trace MeHg, EtHg and Hg(2+) in tap water with a limits of quantification (LOQs) of 0.26-0.45 pg/mL, an RSD (n = 3) < 6% and a recovery of 92-108%...
  48. Ferreira Lima N, Vallverdú Queralt A, Meudec E, Pinasseau L, Verbaere A, Bordignon Luiz M, et al. Quantification of hydroxycinnamic derivatives in wines by UHPLC-MRM-MS. Anal Bioanal Chem. 2018;410:3483-3490 pubmed publisher
    ..The developed method led to an overall improvement in the limits of detection (LODs) and quantification (LOQs) for all the compounds studied in comparison to other methods such as high-performance liquid chromatography with ..
  49. Chen C, Zhu C, Huang J, Zhao X, Deng R, Zhang H, et al. SUMOylation of TARBP2 regulates miRNA/siRNA efficiency. Nat Commun. 2015;6:8899 pubmed publisher
    ..Here we show that TARBP2 is SUMOylated at K52, which can be enhanced by its phosphorylation...
  50. Rocchi R, Simeoni M, Montesano C, Vannutelli G, Curini R, Sergi M, et al. Analysis of new psychoactive substances in oral fluids by means of microextraction by packed sorbent followed by UHPLC-MS/MS. Drug Test Anal. 2017;: pubmed publisher
    ..9% to 107%, accomplishing SWGTOX requirements. Limits of detection (LODs) ranged between 0.005 ng/ml to 0.850 ng/mL and limits of quantification (LOQs) from 0.015 to 2.600 ng/mL.
  51. Eichbaum K, Brinkmann M, Nuesser L, Gembé C, Ohlig M, Buchinger S, et al. In vitro tools for the toxicological evaluation of sediments and dredged materials: intra- and inter-laboratory comparisons of chemical and bioanalytical methods. Environ Sci Pollut Res Int. 2018;25:4037-4050 pubmed publisher
    ..803) with TEQs. Its LOD and LOQ of 0.5 and 0.7 pM 2,3,7,8-TCDD, respectively, approached LOQs of HRGC/HRMS measurements. In contrast, cell lines RTL-W1 and H4IIE-luc produced LODs > 0...
  52. Hanafi A, Dasenaki M, Bletsou A, Thomaidis N. Dissipation rate study and pre-harvest intervals calculation of imidacloprid and oxamyl in exported Egyptian green beans and chili peppers after pestigation treatment. Food Chem. 2018;240:1047-1054 pubmed publisher
    ..47 and 2.6?g/kg in green beans and chili peppers, respectively, while for oxamyl the LOQs were 2.9 and 0.67?g/kg, respectively...
  53. Golovko O, Sauer P, Fedorova G, Kroupova H, Grabic R. Determination of progestogens in surface and waste water using SPE extraction and LC-APCI/APPI-HRPS. Sci Total Environ. 2018;621:1066-1073 pubmed publisher
    ..The method is very sensitive with LOQs ranging from 0.02 to 0.87ng/L...
  54. Kim Y, Yeo J, Lee J, Cho J, Seo D, Kim J, et al. Deletion of human tarbp2 reveals cellular microRNA targets and cell-cycle function of TRBP. Cell Rep. 2014;9:1061-74 pubmed publisher
    b>TRBP functions as both a Dicer cofactor and a PKR inhibitor. However, the role of TRBP in microRNA (miRNA) biogenesis is controversial and its regulation of PKR in mitosis remains unexplored...
  55. Zhou J, Xu J, Cong J, Cai Z, Zhang J, Wang J, et al. Optimization for quick, easy, cheap, effective, rugged and safe extraction of mycotoxins and veterinary drugs by response surface methodology for application to egg and milk. J Chromatogr A. 2018;1532:20-29 pubmed publisher
    ..and 40%-105% for other veterinary drugs), precision (inter-day RSDs < 14%) and sensitivity (LOQs ranged from 0.01 μg/kg to 31 μg/kg), were achieved under the optimized conditions...
  56. Chukwurah E, Willingham V, Singh M, Castillo Azofeifa D, Patel R. Contribution of the two dsRBM motifs to the double-stranded RNA binding and protein interactions of PACT. J Cell Biochem. 2017;: pubmed publisher
    ..PACT-induced PKR activation is negatively regulated by TRBP (Transactivation response element RNA-binding protein), which dissociates from PACT after PACT phosphorylation in ..
  57. Zhou Y, Zeng Z, Yu J, Liu R, Pan L, Zhong Q, et al. [Simultaneous determination of diminazene aceturate and isometamidium chloride residues in cattle tissues by high performance liquid chromatography with solid-phase extraction]. Se Pu. 2017;35:995-1002 pubmed publisher
    ..The detection wavelength of UV was set at 380 nm. The limits of detection (LODs) and the limits of quantification (LOQs) of DIM and ISM in cattle tissues were 0.01 mg/kg and 0.025 mg/kg, respectively...
  58. Liu Y, Liu S, Xu W, Tian G, Zhang J. [Rapid screening of 44 pesticide residues in ginger and scallion by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry]. Se Pu. 2017;35:941-948 pubmed publisher
    ..995 in the linear ranges of the 44 pesticides. The limits of quantification (LOQs, S/N=10) of the 44 pesticides were 2.5-5.0 ?g/kg. At the three spiked levels, the recoveries were between 73...
  59. Li J, Wang J, Qiu H, Li W. [Determination of five nucleotides in infant formula milk powder by modified high performance liquid chromatography]. Se Pu. 2017;35:832-836 pubmed publisher
    ..The recoveries were determined at three spiked levels ranging from 86.9% to 105.7%. The limits of quantification (LOQs) were from 5.6 mg/kg to 8.0 mg/kg. The intra-day and inter-day precisions were 0.5%-1.7% (n=5) and 0.6%-1...
  60. Liao J, Zhou X, Gatignol A, Lu H. Ribosomal proteins L5 and L11 co-operatively inactivate c-Myc via RNA-induced silencing complex. Oncogene. 2014;33:4916-23 pubmed publisher
    ..Immunoprecipitation revealed that RPL5 binds to 3'UTR of c-Myc mRNA and two subunits of RISC, TRBP (HIV-1 TAR RNA-binding protein) and Ago2, mediating the targeting of c-Myc mRNA by miRNAs...
  61. Kozak C, Gatignol A, Graham K, Jeang K, McBride O. Genetic mapping in human and mouse of the locus encoding TRBP, a protein that binds the TAR region of the human immunodeficiency virus (HIV-1). Genomics. 1995;25:66-72 pubmed
    ..Here we demonstrate that this factor is encoded by a gene, TARBP2, that maps to human chromosome 12 and mouse chromosome 15, and we also identify and map one human pseudogene (..
  62. Trettin K, Sinha N, Eckert D, Apple S, Bass B. Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA. Proc Natl Acad Sci U S A. 2017;114:E7939-E7948 pubmed publisher
    Loquacious-PD (Loqs-PD) is required for biogenesis of many endogenous siRNAs in Drosophila In vitro, Loqs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normally refractory to cleavage...
  63. Boulanouar S, Combes A, Mezzache S, Pichon V. Synthesis and application of molecularly imprinted polymers for the selective extraction of organophosphorus pesticides from vegetable oils. J Chromatogr A. 2017;1513:59-68 pubmed publisher
    ..Recoveries were between 73 and 99% with SD values between 4 and 6% in this oil sample. The calculated LOQs (between 0.3 and 2?g/kg) in almond seeds with a SD between 0.1 and 0...
  64. Lin J, Horikawa Y, Tamboli P, Clague J, Wood C, Wu X. Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma. Carcinogenesis. 2010;31:1805-12 pubmed publisher
    ..Future investigation in larger populations and functional characterizations are necessary to validate these results. ..
  65. Arnnok P, Patdhanagul N, Burakham R. Dispersive solid-phase extraction using polyaniline-modified zeolite NaY as a new sorbent for multiresidue analysis of pesticides in food and environmental samples. Talanta. 2017;164:651-661 pubmed publisher
    ..The proposed method gave LODs and LOQs in the ranges of 0.001-1.00mgL-1 and 0.005-2.50mgL-1, respectively...
  66. Xiong L, Yan P, Chu M, Gao Y, Li W, Yang X. A rapid and simple HPLC-FLD screening method with QuEChERS as the sample treatment for the simultaneous monitoring of nine bisphenols in milk. Food Chem. 2018;244:371-377 pubmed publisher
    ..6 to 11.1%. The limits of detection (LODs) and quantification (LOQs) were 1.0-3.1??g/kg and 3.5-9.8??g/kg, respectively...
  67. Fu Q, Yuan Y. Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helicase A (DHX9). Nucleic Acids Res. 2013;41:3457-70 pubmed publisher
    ..Hence, our structural and functional efforts have revealed a potential working model for siRNA recognition by RHA tandem dsRBDs, and together they provide direct structural insights into RISC assembly facilitated by RHA. ..
  68. Lee H, Zhou K, Smith A, Noland C, Doudna J. Differential roles of human Dicer-binding proteins TRBP and PACT in small RNA processing. Nucleic Acids Res. 2013;41:6568-76 pubmed publisher
    ..RNA (dsRNA)-binding proteins, protein activator of PKR (PACT) and trans-activation response RNA-binding protein (TRBP)...
  69. You X, Chen X, Liu F, Hou F, Li Y. Ionic liquid-based air-assisted liquid-liquid microextraction followed by high performance liquid chromatography for the determination of five fungicides in juice samples. Food Chem. 2018;239:354-359 pubmed publisher
    ..4-1.8?gL-1 at a signal-to-noise ratio of 3. The limits of quantification (LOQs) set as the lowest spiking levels with acceptable recovery in juices were 10?gL-1, except for ..
  70. Acevedo R, Orench Rivera N, Quarles K, Showalter S. Helical defects in microRNA influence protein binding by TAR RNA binding protein. PLoS ONE. 2015;10:e0116749 pubmed publisher
    ..Here, we focus on the tandem-dsRBDs of TRBP, which have been shown to bind dsRNA tightly...
  71. Ye M, Zhu Y. Simultaneous Determination and Investigation of Nine Fungicides in Fruits Using Diethylenetriamine-Functional Magnetic Core-Shell Polymer Modified Graphene Oxide as an Efficient Adsorbent Coupled to UPLC-HRMS. Int J Mol Sci. 2017;18: pubmed publisher
    ..Moreover, the limits of quantitation (LOQs) of the proposed approach were in the range of 0.01 to 0.30 ?g/kg under the optimum conditions...
  72. Ye J. [A novel ship-borne positive pressure solid phase extraction device to enrich organo chlorinated and pyrethroid pesticides in seawater]. Se Pu. 2017;35:907-911 pubmed publisher
    ..The linearity of the method was good with the correlation coefficient more than 0.9. The limits of quantification (LOQs) were 0.8-6 ng/L. The recoveries of the pesticides at three spiked levels (3 parallel samples) were 86.1%-95...
  73. Zhang J, Wan H, Zhang H. [Determination of the plant growth regulators in batatas by matrix solid-phase dispersive extraction and high performance liquid chromatography with tandem mass spectrometry]. Se Pu. 2017;35:963-969 pubmed publisher
    ..2-204.0 ng/g (paclobutrazol) with the correlation coefficients larger than 0.96. The limits of quantification (LOQs) were 0.1-0.3 ng/g...
  74. Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
    ..They are generated following transcription and cleavage by the DROSHA/DGCR8 and DICER/TRBP/PACT complexes...
  75. Jin X, Guo X, Zhu D, Ayaz M, Zheng Y. miRNA profiling in the mice in response to Echinococcus multilocularis infection. Acta Trop. 2017;166:39-44 pubmed publisher
    ..disturbed the expression of 4 of 10 genes essential to miRNA biogenesis in the mouse liver, including ago1, ago4, tarbp2 and xrn2. Comparative analysis of deep sequencing data identified 46 differentially expressed miRNAs with 93...
  76. Shi Y, Zuo D, Wang X, Han M, Wu Y. shRNA?mediated silencing of TARBP2 inhibits NCI?H1299 non?small cell lung cancer cell invasion and migration via the JNK/STAT3/AKT pathway. Mol Med Rep. 2016;14:3725-30 pubmed publisher
    ..aimed to investigate the effects and mechanisms of TAR (human immunodeficiency virus 1) RNA binding protein 2 (TARBP2) in the invasion and migration of non?small cell lung cancer in vitro...
  77. Feng X, Yu J, Pan L, Song G, Zhang H. Dissipation and Residues of Dichlorprop-P and Bentazone in Wheat-Field Ecosystem. Int J Environ Res Public Health. 2016;13: pubmed publisher
    ..The average recoveries of this method ranged from 72.9% to 108.7%, and the limits of quantification (LOQs) were 2.5-12 ?g/kg...