TADA3

Summary

Gene Symbol: TADA3
Description: transcriptional adaptor 3
Alias: ADA3, NGG1, STAF54, TADA3L, hADA3, transcriptional adapter 3, ADA3 homolog, ADA3-like protein, alteration/deficiency in activation 3
Species: human
Products:     TADA3

Top Publications

  1. Li C, Ai N, Dinh G, Welsh W, Chen J. Human ADA3 regulates RARalpha transcriptional activity through direct contact between LxxLL motifs and the receptor coactivator pocket. Nucleic Acids Res. 2010;38:5291-303 pubmed publisher
    ..We show that hADA3 interacts directly with RAR? in a hormone-dependent manner and this interaction contributes to RAR? ..
  2. Ogryzko V, Kotani T, Zhang X, Schiltz R, Howard T, Yang X, et al. Histone-like TAFs within the PCAF histone acetylase complex. Cell. 1998;94:35-44 pubmed
    ..Moreover, the PCAF complex has a novel subunit with WD40 repeats having a similarity to hTAF(II)100. ..
  3. Mohibi S, Gurumurthy C, Nag A, Wang J, Mirza S, Mian Y, et al. Mammalian alteration/deficiency in activation 3 (Ada3) is essential for embryonic development and cell cycle progression. J Biol Chem. 2012;287:29442-56 pubmed publisher
    b>Ada3 protein is an essential component of histone acetyl transferase containing coactivator complexes conserved from yeast to human...
  4. Li C, Dinh G, Zhang A, Chen J. Ankyrin repeats-containing cofactors interact with ADA3 and modulate its co-activator function. Biochem J. 2008;413:349-57 pubmed publisher
    ..study, we provide new evidence to suggest further that ANCO-1 and ANCO-2 also interact with the co-activator ADA3 (alteration/deficiency in activation 3)...
  5. Wang T, Kobayashi T, Takimoto R, Denes A, Snyder E, El Deiry W, et al. hADA3 is required for p53 activity. EMBO J. 2001;20:6404-13 pubmed
    ..Using a novel yeast p53 dissociator assay, we identify hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity...
  6. Kumar A, Zhao Y, Meng G, Zeng M, Srinivasan S, Delmolino L, et al. Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3. Mol Cell Biol. 2002;22:5801-12 pubmed
    ..The HPV oncoprotein E6 is essential for oncogenic transformation. We identify here hADA3, human homologue of the yeast transcriptional coactivator yADA3, as a novel E6-interacting protein and a target of ..
  7. Zeng M, Kumar A, Meng G, Gao Q, Dimri G, Wazer D, et al. Human papilloma virus 16 E6 oncoprotein inhibits retinoic X receptor-mediated transactivation by targeting human ADA3 coactivator. J Biol Chem. 2002;277:45611-8 pubmed
    ..We have recently isolated hADA3, the human homologue of yeast transcriptional co-activator yADA3, as a novel E6 target...
  8. Meng G, Zhao Y, Nag A, Zeng M, Dimri G, Gao Q, et al. Human ADA3 binds to estrogen receptor (ER) and functions as a coactivator for ER-mediated transactivation. J Biol Chem. 2004;279:54230-40 pubmed
    We have recently identified the hADA3 protein, the human homologue of yeast transcriptional coactivator yADA3, as a novel HPV16 E6 target...
  9. Benecke A, Gaudon C, Garnier J, vom Baur E, Chambon P, Losson R. ADA3-containing complexes associate with estrogen receptor alpha. Nucleic Acids Res. 2002;30:2508-14 pubmed
    ..We have previously implicated the yeast ADA3 protein in transcriptional activation by estrogen and retinoid X receptors in yeast and mammalian cells...

More Information

Publications44

  1. Germaniuk Kurowska A, Nag A, Zhao X, Dimri M, Band H, Band V. Ada3 requirement for HAT recruitment to estrogen receptors and estrogen-dependent breast cancer cell proliferation. Cancer Res. 2007;67:11789-97 pubmed
    ..Here, we examined the role of endogenous Ada3 to recruit histone acetyl transferases (HAT) to an ER-responsive promoter and its role in estrogen-dependent cell ..
  2. Nag A, Germaniuk Kurowska A, Dimri M, Sassack M, Gurumurthy C, Gao Q, et al. An essential role of human Ada3 in p53 acetylation. J Biol Chem. 2007;282:8812-20 pubmed
    ..We and others have recently identified hAda3 (human homologue of yeast alteration/deficiency in activation 3) as a p53-interacting protein and enhancer of p53 ..
  3. Rössl A, Denoncourt A, Lin M, Downey M. A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs. J Biol Chem. 2019;294:6227-6239 pubmed publisher
    ..In particular, we find a key role for the Ada2 and Ada3 subunits in regulating acetylations on our fusion substrate...
  4. Zhang S, Xu L, Tang Z, Wang H, Gu J, Sun B, et al. Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer. Pathol Res Pract. 2019;: pubmed publisher
    Alteration/Deficiency in Activation 3 (ADA3), the human homologue of yeast ADA3, is involved in a variety of cell biological processes and plays an important role in tumorigenesis...
  5. Burke T, Miller J, Grant P. Direct inhibition of Gcn5 protein catalytic activity by polyglutamine-expanded ataxin-7. J Biol Chem. 2013;288:34266-75 pubmed publisher
    ..ataxin-7 directly bound the Gcn5 catalytic core of SAGA while in association with its regulatory proteins, Ada2 and Ada3. This caused a significant decrease in Gcn5 histone acetyltransferase activity in vitro and in vivo at two SAGA-..
  6. Brasacchio D, Busuttil R, Noori T, Johnstone R, Boussioutas A, Trapani J. Down-regulation of a pro-apoptotic pathway regulated by PCAF/ADA3 in early stage gastric cancer. Cell Death Dis. 2018;9:442 pubmed publisher
    ..Given that PCAF and its binding partner transcriptional adaptor protein 3 (ADA3) were recently shown to regulate the intrinsic (mitochondrial) pathway to apoptosis via epigenetic regulation of ..
  7. Torres Zelada E, Stephenson R, Alpsoy A, Anderson B, Swanson S, Florens L, et al. The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability. J Cell Sci. 2019;132: pubmed publisher
    ..Here, we show that only the Ada2b-PB isoform is in SAGA; in contrast, Ada2b-PA associates with Gcn5, Ada3, Sgf29 and Chiffon, forming the Chiffon histone acetyltransferase (CHAT) complex...
  8. Mohibi S, Srivastava S, Wang France J, Mirza S, Zhao X, Band H, et al. Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation. J Biol Chem. 2015;290:28299-310 pubmed publisher
    b>ADA3 (alteration/deficiency in activation 3) is a conserved component of several transcriptional co-activator and histone acetyltransferase (HAT) complexes...
  9. Ringel A, Cieniewicz A, Taverna S, Wolberger C. Nucleosome competition reveals processive acetylation by the SAGA HAT module. Proc Natl Acad Sci U S A. 2015;112:E5461-70 pubmed publisher
    ..known as the histone acetyltransferase (HAT) module that contains the HAT, Gcn5, bound to Sgf29, Ada2, and Ada3. Sgf29 contains a tandem Tudor domain that recognizes H3K4me3-containing peptides and is required for histone ..
  10. Chand V, Nandi D, Mangla A, Sharma P, Nag A. Tale of a multifaceted co-activator, hADA3: from embryogenesis to cancer and beyond. Open Biol. 2016;6: pubmed publisher
    ..witnessed exciting findings leading to considerable expansion in research related to the biology and regulation of hADA3. Embryonic lethality in homozygous knockout Ada3 mouse signifies the importance of this gene product during early ..
  11. Griffin N, Sharma G, Zhao X, Mirza S, Srivastava S, Dave B, et al. ADA3 regulates normal and tumor mammary epithelial cell proliferation through c-MYC. Breast Cancer Res. 2016;18:113 pubmed
    We have established the critical role of ADA3 as a coactivator of estrogen receptor (ER), as well as its role in cell cycle progression...
  12. Zhang X, Varthi M, Sykes S, Phillips C, Warzecha C, Zhu W, et al. The putative cancer stem cell marker USP22 is a subunit of the human SAGA complex required for activated transcription and cell-cycle progression. Mol Cell. 2008;29:102-11 pubmed publisher
    ..In support of a functional role within the Polycomb/cancer stem cell signature, USP22 is required for appropriate progression through the cell cycle. ..
  13. Ebmeier C, Taatjes D. Activator-Mediator binding regulates Mediator-cofactor interactions. Proc Natl Acad Sci U S A. 2010;107:11283-8 pubmed publisher
    ..VP16-Mediator. These data define an expanded role for activators in regulating gene expression in humans and suggest that distinct, activator-induced structural shifts regulate Mediator function in gene-specific ways. ..
  14. Brand M, Yamamoto K, Staub A, Tora L. Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction. J Biol Chem. 1999;274:18285-9 pubmed
    ..Furthermore, we identified four other previously uncharacterized subunits of TFTC: hADA3, hTAFII150, hSPT3, and hPAF65beta...
  15. Zencir S, Sike A, Dobson M, Ayaydin F, Boros I, Topcu Z. Identification of transcriptional and phosphatase regulators as interaction partners of human ADA3, a component of histone acetyltransferase complexes. Biochem J. 2013;450:311-20 pubmed publisher
    ..we used a yeast two-hybrid approach to screen a human fetal cDNA library for proteins that interacted with hADA3 (human ADA3)...
  16. Yang M, Waterman M, Brachmann R. hADA2a and hADA3 are required for acetylation, transcriptional activity and proliferative effects of beta-catenin. Cancer Biol Ther. 2008;7:120-8 pubmed
    ..Here we show that two members of histone acetyltransferase complexes without enzymatic activity, hADA2a and hADA3, are required for full activity of beta-catenin...
  17. Brasacchio D, Noori T, House C, Brennan A, Simpson K, Susanto O, et al. A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage. Cell Death Differ. 2014;21:748-60 pubmed publisher
    ..Twenty-two shRNAmiRs were identified in the positive-selection screen including two, PCAF and ADA3, whose gene products are known to reside in the same epigenetic regulatory complexes...
  18. Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati M, Qin J, et al. The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily. Mol Cell. 1998;2:869-75 pubmed
    ..We discuss the possibility that PAF400 may play a role in signaling of DNA damage to p53 by stimulation of p53 acetylation. ..
  19. Brand M, Moggs J, Oulad Abdelghani M, Lejeune F, Dilworth F, Stevenin J, et al. UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation. EMBO J. 2001;20:3187-96 pubmed
    ..Thus, our experiments reveal a molecular link between DNA damage recognition and chromatin modification. ..
  20. Palhan V, Chen S, Peng G, Tjernberg A, Gamper A, Fan Y, et al. Polyglutamine-expanded ataxin-7 inhibits STAGA histone acetyltransferase activity to produce retinal degeneration. Proc Natl Acad Sci U S A. 2005;102:8472-7 pubmed
  21. Gamper A, Roeder R. Multivalent binding of p53 to the STAGA complex mediates coactivator recruitment after UV damage. Mol Cell Biol. 2008;28:2517-27 pubmed publisher
    ..Based on our data, we propose a cooperative and modular binding mode for the recruitment of coactivator complexes to promoters. ..
  22. Mohibi S, Srivastava S, Bele A, Mirza S, Band H, Band V. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol. 2016;36:2487-502 pubmed publisher
    Alteration/deficiency in activation 3 (ADA3) is an essential component of specific histone acetyltransferase (HAT) complexes...
  23. Srivastava S, Mohibi S, Mirza S, Band H, Band V. Epidermal Growth Factor Receptor activation promotes ADA3 acetylation through the AKT-p300 pathway. Cell Cycle. 2017;16:1515-1525 pubmed publisher
    The ADA3 (Alteration/Deficiency in Activation 3) protein is an essential adaptor component of several Lysine Acetyltransferase (KAT) complexes involved in chromatin modifications...
  24. Mirza S, Rakha E, Alshareeda A, Mohibi S, Zhao X, Katafiasz B, et al. Cytoplasmic localization of alteration/deficiency in activation 3 (ADA3) predicts poor clinical outcome in breast cancer patients. Breast Cancer Res Treat. 2013;137:721-31 pubmed publisher
    ..Given previous findings that ADA3 is a critical component of HAT complexes that regulate ER function and evidence that overexpression of other ER ..
  25. Holmlund T, Lindberg M, Grander D, Wallberg A. GCN5 acetylates and regulates the stability of the oncoprotein E2A-PBX1 in acute lymphoblastic leukemia. Leukemia. 2013;27:578-85 pubmed publisher
    ..In addition, we show that the E3 ubiquitin ligase HDM2 potentiates the degradation of E2A-PBX1. We suggest that dynamic regulation of E2A-PBX1 protein levels in vivo has a fundamental role in ALL. ..
  26. Wang Y, Faiola F, Xu M, Pan S, Martinez E. Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. J Biol Chem. 2008;283:33808-15 pubmed publisher
    ..human ATAC complexes incorporate in addition to GCN5 or PCAF (GCN5/PCAF), other epigenetic coregulators (ADA2-A, ADA3, STAF36, and WDR5), cofactors of chromatin assembly/remodeling and DNA replication machineries (POLE3/CHRAC17 and ..
  27. Caliskan G, Baris I, Ayaydin F, Dobson M, Senarisoy M, Boros I, et al. Che1/AATF interacts with subunits of the histone acetyltransferase core module of SAGA complexes. PLoS ONE. 2017;12:e0189193 pubmed publisher
    General Control Non-derepressible 5 (GCN5) and Alteration/Deficiency in Activation 2 and 3 proteins (ADA2 and ADA3, respectively) are subunits of the Histone AcetylTransferase (HAT) module of SAGA- and ATAC-type co-activators...
  28. Shamanin V, Sekaric P, Androphy E. hAda3 degradation by papillomavirus type 16 E6 correlates with abrogation of the p14ARF-p53 pathway and efficient immortalization of human mammary epithelial cells. J Virol. 2008;82:3912-20 pubmed publisher
    ..However, the requirement for p53 inactivation has been debated. Another E6 target is the hAda3 protein, a p53 coactivator and a component of histone acetyltransferase complexes...
  29. Brasacchio D, Alsop A, Noori T, Lufti M, Iyer S, Simpson K, et al. Epigenetic control of mitochondrial cell death through PACS1-mediated regulation of BAX/BAK oligomerization. Cell Death Differ. 2017;24:961-970 pubmed publisher
    PCAF and ADA3 associate within the same macromolecular complexes to control the transcription of many genes, including some that regulate apoptosis...
  30. Struhl K, Moqtaderi Z. The TAFs in the HAT. Cell. 1998;94:1-4 pubmed
  31. Riss A, Scheer E, Joint M, Trowitzsch S, Berger I, Tora L. Subunits of ADA-two-A-containing (ATAC) or Spt-Ada-Gcn5-acetyltrasferase (SAGA) Coactivator Complexes Enhance the Acetyltransferase Activity of GCN5. J Biol Chem. 2015;290:28997-9009 pubmed publisher
    ..The HAT module of ATAC is composed of GCN5, ADA2a, ADA3, and SGF29, whereas in the SAGA HAT module ADA2b is present instead of ADA2a...
  32. Sekaric P, Shamanin V, Luo J, Androphy E. hAda3 regulates p14ARF-induced p53 acetylation and senescence. Oncogene. 2007;26:6261-8 pubmed
    ..p14ARF-induced senescence of human mammary epithelial cells (MEC) is associated with p53 acetylation and requires hAda3, a component of histone acetyltransferase complexes and a p53 transcriptional coactivator...
  33. Hu Y, Ye F, Lu W, Hong D, Wan X, Xie X. HPV16 E6-induced and E6AP-dependent inhibition of the transcriptional coactivator hADA3 in human cervical carcinoma cells. Cancer Invest. 2009;27:298-306 pubmed publisher
    ..whether there exists an additional pathway of E6 that is independent of direct P53 degradation and whether hADA3, a transcriptional coactivator, is involved in this process...
  34. Martinez E, Palhan V, Tjernberg A, Lymar E, Gamper A, Kundu T, et al. Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. Mol Cell Biol. 2001;21:6782-95 pubmed
  35. Liu X, Tesfai J, Evrard Y, Dent S, Martinez E. c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation. J Biol Chem. 2003;278:20405-12 pubmed
    ..Thus, TRRAP might function as an adaptor within the STAGA complex, which helps recruit GCN5 HAT activity to Myc during transcription activation. ..