SUPT3H

Summary

Gene Symbol: SUPT3H
Description: SPT3 homolog, SAGA and STAGA complex component
Alias: SPT3, SPT3L, transcription initiation protein SPT3 homolog, SPT3-like protein, suppressor of Ty 3 homolog
Species: human
Products:     SUPT3H

Top Publications

  1. Martinez E, Kundu T, Fu J, Roeder R. A human SPT3-TAFII31-GCN5-L acetylase complex distinct from transcription factor IID. J Biol Chem. 1998;273:23781-5 pubmed
    In yeast, SPT3 is a component of the multiprotein SPT-ADA-GCN5 acetyltransferase (SAGA) complex that integrates proteins with transcription coactivator/adaptor functions (ADAs and GCN5), histone acetyltransferase activity (GCN5), and core ..
  2. Palhan V, Chen S, Peng G, Tjernberg A, Gamper A, Fan Y, et al. Polyglutamine-expanded ataxin-7 inhibits STAGA histone acetyltransferase activity to produce retinal degeneration. Proc Natl Acad Sci U S A. 2005;102:8472-7 pubmed
    ..Here, we report that ataxin-7 is an integral component of the mammalian STAGA (SPT3-TAF9-ADA-GCN5 acetyltransferase) transcription coactivator complex, interacts directly with the GCN5 histone ..
  3. Martinez E, Palhan V, Tjernberg A, Lymar E, Gamper A, Kundu T, et al. Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. Mol Cell Biol. 2001;21:6782-95 pubmed
    ..SAGA-like complexes (PCAF complex, TFTC [TATA-binding-protein-free TAF(II)-containing complex], and STAGA [SPT3-TAF(II)31-GCN5L acetylase])...
  4. Brand M, Moggs J, Oulad Abdelghani M, Lejeune F, Dilworth F, Stevenin J, et al. UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation. EMBO J. 2001;20:3187-96 pubmed
    ..Thus, our experiments reveal a molecular link between DNA damage recognition and chromatin modification. ..
  5. Cavusoglu N, Brand M, Tora L, Van Dorsselaer A. Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis. Proteomics. 2003;3:217-23 pubmed
    ..already described subunits (TRRAP, GCN5, SAP130/KIA0017, TAF(II)150, TAF(II)135, TAF(II)100, TAF(II)80, TAF(II)20, SPT3 and PAF65beta). Moreover, a good coverage of these sequences was obtained...
  6. Brand M, Yamamoto K, Staub A, Tora L. Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction. J Biol Chem. 1999;274:18285-9 pubmed
    ..Thus, the polypeptide composition of TFTC suggests that TFTC is recruited to chromatin templates by activators to acetylate histones and thus may potentiate initiation and activation of transcription. ..
  7. Arumugam B, Vishal M, Shreya S, Malavika D, Rajpriya V, He Z, et al. Parathyroid hormone-stimulation of Runx2 during osteoblast differentiation via the regulation of lnc-SUPT3H-1:16 (RUNX2-AS1:32) and miR-6797-5p. Biochimie. 2019;158:43-52 pubmed publisher
    ..Using bioinformatics tools, we identified 17 lncRNAs originating from human Runx2 gene. Among these, lnc-SUPT3H-1:16 (RUNX2-AS1:32) expression was highly up-regulated by the 7 d PTH-treatment in hBMSCs...
  8. Molin A, Lopez Cazaux S, Pichon O, Vincent M, Isidor B, Le Caignec C. Patients with isolated oligo/hypodontia caused by RUNX2 duplication. Am J Med Genet A. 2015;167:1386-90 pubmed publisher
    ..two cousins with a duplication including the entire RUNX2 sequence in addition to MIR586, CLIC5 and the 5' half of SUPT3H have been reported. These patients presented with metopic synostosis and hypodontia...
  9. Durand A, Bonnet J, Fournier M, Chavant V, Schultz P. Mapping the deubiquitination module within the SAGA complex. Structure. 2014;22:1553-9 pubmed
    ..protein (TBP)-interacting subunit Spt8 was found to be located close to the DUB but in a different domain than Spt3, also known to contact TBP...

More Information

Publications35

  1. Han Y, Luo J, Ranish J, Hahn S. Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex. EMBO J. 2014;33:2534-46 pubmed publisher
    ..SAGA-TBP binding involves a network of interactions between subunits Spt3, Spt8, Spt20, and Spt7...
  2. Barutcu A, Tai P, Wu H, Gordon J, Whitfield T, Dobson J, et al. The bone-specific Runx2-P1 promoter displays conserved three-dimensional chromatin structure with the syntenic Supt3h promoter. Nucleic Acids Res. 2014;42:10360-72 pubmed publisher
    ..Mining the ENCODE database revealed interactions between Runx2-P1 and Supt3h promoters in several non-mesenchymal human cell lines...
  3. Lin Y, Mallen St Clair J, Wang G, Luo J, Palma Diaz F, Lai C, et al. p38 MAPK mediates epithelial-mesenchymal transition by regulating p38IP and Snail in head and neck squamous cell carcinoma. Oral Oncol. 2016;60:81-9 pubmed publisher
    ..p38 binds to and stabilizes p38IP, a subunit of histone SPT3-TAF9-GCN5 acetyltransferase (STAGA), resulting in enhanced transcription of Snail...
  4. Castano Betancourt M, Evans D, Ramos Y, Boer C, Metrustry S, Liu Y, et al. Novel Genetic Variants for Cartilage Thickness and Hip Osteoarthritis. PLoS Genet. 2016;12:e1006260 pubmed publisher
    ..rs2862851), PIK3R1 (rs10471753), SLBP/FGFR3 (rs2236995), and TREH/DDX6 (rs496547), while the other two (DOT1L and SUPT3H/RUNX2) were previously identified...
  5. Adachi R, Asano Y, Ogawa K, Oonishi M, Tanaka Y, Kawamoto T. Pharmacological characterization of synthetic serine palmitoyltransferase inhibitors by biochemical and cellular analyses. Biochem Biophys Res Commun. 2018;497:1171-1176 pubmed publisher
    ..These SPT inhibitors exhibited dual inhibitory activities against SPT2 and SPT3. We used a fluorescent-labelled probe to molecularly assess the inhibitory mechanism and revealed its binding to ..
  6. Zhu L, Gao S, Zhang H, Huang H, Jiang L. Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3. Appl Biochem Biotechnol. 2018;184:155-167 pubmed publisher
    ..Herein, we adopted a novel random mutagenesis technique called RAISE to manipulate the transcription regulator SPT3 of Saccharomyces cerevisiae to improve cell lead tolerance...
  7. Mendizabal I, Marigorta U, Lao O, Comas D. Adaptive evolution of loci covarying with the human African Pygmy phenotype. Hum Genet. 2012;131:1305-17 pubmed publisher
    ..a significant excess of genes with pivotal roles in bone homeostasis, such as PPPT3B and the height associated SUPT3H-RUNX2. We hypothesize that skeletal remodeling could be a key biological process underlying the Pygmy phenotype...
  8. de Vries P, Boender J, Sonneveld M, Rivadeneira F, Ikram M, Rottensteiner H, et al. Genetic variants in the ADAMTS13 and SUPT3H genes are associated with ADAMTS13 activity. Blood. 2015;125:3949-55 pubmed publisher
    ..0; P = 9.6 × 10(-21)) and rs139911703 (β, -11.6; P = 3.6 × 10(-8)). In addition, rs10456544 in SUPT3H was associated with a 4.2 increase in ADAMTS13 activity (P = 1.13.6 × 10(-8))...
  9. Nakajima M, Takahashi A, Tsuji T, Karasugi T, Baba H, Uchida K, et al. A genome-wide association study identifies susceptibility loci for ossification of the posterior longitudinal ligament of the spine. Nat Genet. 2014;46:1012-6 pubmed publisher
    ..Analyses of gene expression in and around the loci suggested that several genes are involved in OPLL etiology through membranous and/or endochondral ossification processes. Our results bring new insight to the etiology of OPLL...
  10. Santos P, Hohne J, Schlattmann P, Konig I, Uchanska Ziegler B, Ziegler A. Assessment of transmission distortion on chromosome 6p in healthy individuals using tagSNPs. Eur J Hum Genet. 2009;17:1182-9 pubmed publisher
    ..and wide-ranging skewed segregation in a 730 kb long region containing the transcription factor-encoding genes SUPT3H and RUNX2, as well as the microRNA locus MIRN586...
  11. Yu J, Madison J, Mundlos S, Winston F, Olsen B. Characterization of a human homologue of the Saccharomyces cerevisiae transcription factor spt3 (SUPT3H). Genomics. 1998;53:90-6 pubmed
    ..We report the cloning of the gene encoding the human homologue of Spt3, SUPT3H, and its initial functional analysis...
  12. Ogryzko V, Kotani T, Zhang X, Schiltz R, Howard T, Yang X, et al. Histone-like TAFs within the PCAF histone acetylase complex. Cell. 1998;94:35-44 pubmed
    ..Moreover, the PCAF complex has a novel subunit with WD40 repeats having a similarity to hTAF(II)100. ..
  13. Zeggini E, Panoutsopoulou K, Southam L, Rayner N, Day Williams A, Lopes M, et al. Identification of new susceptibility loci for osteoarthritis (arcOGEN): a genome-wide association study. Lancet. 2012;380:815-23 pubmed publisher
    ..Our findings provide insight into the genetics of arthritis and identify new pathways that might be amenable to future therapeutic intervention. arcOGEN was funded by a special purpose grant from Arthritis Research UK. ..
  14. Holliday E, Maguire J, Evans T, Koblar S, Jannes J, Sturm J, et al. Common variants at 6p21.1 are associated with large artery atherosclerotic stroke. Nat Genet. 2012;44:1147-51 pubmed publisher
    ..9×10(-4); discovery and replication combined OR=1.21, P=4.7×10(-8)). This study identifies a genetic risk locus for LAA and shows how analyzing etiological subtypes may better identify genetic risk alleles for ischemic stroke. ..
  15. Chen J, Luo Q, Yuan Y, Huang X, Cai W, Li C, et al. Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion. Mol Cell Biol. 2010;30:5621-35 pubmed publisher
    ..This work now links mechanistically Pygo2's role in histone modification to its enhancement of the Wnt-dependent transcriptional program and cancer stem-like cell expansion. ..
  16. Zhao Y, Lang G, Ito S, Bonnet J, Metzger E, Sawatsubashi S, et al. A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing. Mol Cell. 2008;29:92-101 pubmed publisher
    ..Thus, the deubiquitinase activity of the TFTC/STAGA HAT complex is necessary to counteract heterochromatin silencing and acts as a positive cofactor for activation by nuclear receptors in vivo. ..
  17. Helmlinger D, Hardy S, Abou Sleymane G, Eberlin A, Bowman A, Gansmuller A, et al. Glutamine-expanded ataxin-7 alters TFTC/STAGA recruitment and chromatin structure leading to photoreceptor dysfunction. PLoS Biol. 2006;4:e67 pubmed
    ..We show here that TFTC/STAGA complexes purified from SCA7 mice have normal TRRAP, GCN5, TAF12, and SPT3 levels and that their histone or nucleosomal acetylation activities are unaffected...
  18. Liu X, Tesfai J, Evrard Y, Dent S, Martinez E. c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation. J Biol Chem. 2003;278:20405-12 pubmed
    ..and GCN5 via direct physical interactions of its N-terminal activation/transformation domain with the human STAGA (SPT3-TAF-GCN5 acetylase) coactivator complex...
  19. Holmlund T, Lindberg M, Grander D, Wallberg A. GCN5 acetylates and regulates the stability of the oncoprotein E2A-PBX1 in acute lymphoblastic leukemia. Leukemia. 2013;27:578-85 pubmed publisher
    ..In the current work we report, for the first time, a physical and functional interaction between the SPT3-TAFII31-GCN5L acetylase (STAGA) complex and E2A-PBX1...
  20. Baptista T, Grünberg S, Minoungou N, Koster M, Timmers H, Hahn S, et al. SAGA Is a General Cofactor for RNA Polymerase II Transcription. Mol Cell. 2017;68:130-143.e5 pubmed publisher
    ..We also find that the acetyltransferase Gcn5 synergizes with Spt3 to promote global transcription and that Spt3 functions to stimulate TBP recruitment at all tested genes...
  21. Nakamura Y, Kayano H, Kakegawa E, Miyazaki H, Nagai T, Uchida Y, et al. Identification of SUPT3H as a novel 8q24/MYC partner in blastic plasmacytoid dendritic cell neoplasm with t(6;8)(p21;q24) translocation. Blood Cancer J. 2015;5:e301 pubmed publisher
  22. Speek M. Antisense promoter of human L1 retrotransposon drives transcription of adjacent cellular genes. Mol Cell Biol. 2001;21:1973-85 pubmed
    ..These results indicate that many L1Hs contain active ASPs which are capable of interfering with normal gene expression, and this type of transcriptional control may be widespread. ..
  23. Gamper A, Roeder R. Multivalent binding of p53 to the STAGA complex mediates coactivator recruitment after UV damage. Mol Cell Biol. 2008;28:2517-27 pubmed publisher
    ..Based on our data, we propose a cooperative and modular binding mode for the recruitment of coactivator complexes to promoters. ..
  24. Liu X, Vorontchikhina M, Wang Y, Faiola F, Martinez E. STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation. Mol Cell Biol. 2008;28:108-21 pubmed
    ..Here we report physical interactions between the human HAT complex STAGA (SPT3-TAF9-GCN5-acetylase) and a "core" form of the Mediator complex during transcription activation by the MYC ..
  25. Vassilev A, Yamauchi J, Kotani T, Prives C, Avantaggiati M, Qin J, et al. The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily. Mol Cell. 1998;2:869-75 pubmed
    ..We discuss the possibility that PAF400 may play a role in signaling of DNA damage to p53 by stimulation of p53 acetylation. ..
  26. Demény M, Soutoglou E, Nagy Z, Scheer E, Janoshazi A, Richardot M, et al. Identification of a small TAF complex and its role in the assembly of TAF-containing complexes. PLoS ONE. 2007;2:e316 pubmed