STX4

Summary

Gene Symbol: STX4
Description: syntaxin 4
Alias: STX4A, p35-2, renal carcinoma antigen NY-REN-31, syntaxin 4A (placental), syntaxin-4
Species: human

Top Publications

  1. pmc Molecular and functional analysis of the LYS1 gene of Candida albicans
    R Garrad
    Department of Microbiology, Miami University, Oxford, Ohio 45056
    Infect Immun 62:5027-31. 1994
  2. ncbi Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion
    G L Reed
    Cardiovascular Biology Laboratory, Harvard School of Public Health, Cardiac Unit, Massachusetts General Hospital, Boston, MA, USA
    Blood 93:2617-26. 1999
  3. pmc The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth
    C A Ghiani
    Mental Retardation Research Center, Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA 90095 7332, USA
    Mol Psychiatry 15:115, 204-15. 2010
  4. ncbi Protease-activated receptor-1 activation of endothelial cells induces protein kinase Calpha-dependent phosphorylation of syntaxin 4 and Munc18c: role in signaling p-selectin expression
    Jian Fu
    Department of Pharmacology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 280:3178-84. 2005
  5. pmc Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2
    M N Jagadish
    CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia
    Biochem J 317:945-54. 1996
  6. doi Syntaxins 3 and 4 mediate vesicular trafficking of α5β1 and α3β1 integrins and cancer cell migration
    Paul Day
    Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA
    Int J Oncol 39:863-71. 2011
  7. doi Aberrant localization of fusion receptors involved in regulated exocytosis in salivary glands of Sjögren's syndrome patients is linked to ectopic mucin secretion
    María José Barrera
    Faculty of Medicine, Institute of Biomedical Sciences ICBM, University of Chile, Postal Code 8389100, Santiago, Chile
    J Autoimmun 39:83-92. 2012
  8. doi SNARE protein expression and localization in human cytotoxic T lymphocytes
    Varsha Pattu
    Institute of Physiology, Saarland University, Homburg, Germany
    Eur J Immunol 42:470-5. 2012
  9. doi The impact of extracellular syntaxin4 on HaCaT keratinocyte behavior
    Nanako Kadono
    Department of Bioscience, Kwansei Gakuin University, Japan
    Biochem Biophys Res Commun 417:1200-5. 2012
  10. doi Integrating interacting drugs and genetic variations to improve the predictability of warfarin maintenance dose in Chinese patients
    Shi long Zhong
    Medical Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Institute of Clinical Pharmacology, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, People s Republic of China
    Pharmacogenet Genomics 22:176-82. 2012

Research Grants

  1. Structure and Function of a Platelet SNARE Complex
    Steven Freedman; Fiscal Year: 2003

Scientific Experts

  • C A Ghiani
  • F Mollinedo
  • Paola Massimi
  • A M Band
  • Steven J Freedman
  • G N Patrick
  • G L Reed
  • Akane Imai
  • Brian J Morris
  • Kelly J Veale
  • Shi long Zhong
  • Rachael Z Murray
  • Carolin Offenhäuser
  • Satoru Nogami
  • María José Barrera
  • Varsha Pattu
  • Nanako Kadono
  • Dan Xu
  • Shu guang Lin
  • Min Yang
  • Yuan Liu
  • Qiu xiong Lin
  • Hong Hong Tan
  • Xi Yong Yu
  • Dean A Wiseman
  • Francine Z Marques
  • David E James
  • Jeffrey E Pessin
  • Paul Day
  • Cristiana Perrotta
  • Frances J Evesson
  • Ponnuswamy Mohanasundaram
  • Matthew J Kennedy
  • Nia J Bryant
  • Gregory M Cooper
  • Wei Tian
  • Cristiana Brochetta
  • Shu Hong Hu
  • Jerome Vicogne
  • Seng Hui Low
  • Jian Fu
  • Tomohiko Miyata
  • Swathi K Rao
  • Thierry Galli
  • Michiko Nakano
  • Veronique Proux-Gillardeaux
  • Sachie Satoh
  • Akira Terano
  • M Kawanishi
  • Hiromichi Shirataki
  • Guangmu Li
  • Tsugumichi Saito
  • Charlotte H Widberg
  • Amanda Hoehn Wyman
  • Sonia Martinez-Arca
  • Todd D Schraw
  • Judith Dent
  • Taishin Takuma
  • Michael R Logan
  • Suzie J Scales
  • Janos Polgar
  • Nasrin Perskvist
  • S Araki
  • Yoji Okugawa
  • Bin Qu
  • Amelina Albornoz
  • Eva C Schwarz
  • Hsiao Hsin Sung
  • Verónica Bahamondes
  • Ulises Urzua
  • Marcela Hermoso
  • Jens Rettig
  • Sergio Aguilera
  • María Julieta González
  • Kiichiro Nakajima
  • Mahantappa Halimani
  • Li Ping Mai
  • Cecilia Alliende
  • Isabel Castro
  • Misty Marshall
  • Bettina Strauss
  • Shruthi S Bhat
  • Takafumi Miyazaki
  • Andrew F G Quest
  • Markus Hoth
  • Claudio Molina
  • L Li
  • Marianela Sánchez
  • Lisa Weins
  • Sergio Gonzalez

Detail Information

Publications66

  1. pmc Molecular and functional analysis of the LYS1 gene of Candida albicans
    R Garrad
    Department of Microbiology, Miami University, Oxford, Ohio 45056
    Infect Immun 62:5027-31. 1994
    ..YpBRG2 has been shown to complement the saccharopine dehydrogenase mutant Stx4-4A of Saccharomyces cerevisiae. Transformants of S...
  2. ncbi Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion
    G L Reed
    Cardiovascular Biology Laboratory, Harvard School of Public Health, Cardiac Unit, Massachusetts General Hospital, Boston, MA, USA
    Blood 93:2617-26. 1999
    ..We found that platelet membranes contain a full complement of interacting proteins-VAMP, SNAP-25, and syntaxin 4-that are necessary for vesicle docking and fusion with the plasma membrane...
  3. pmc The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth
    C A Ghiani
    Mental Retardation Research Center, Jane and Terry Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA 90095 7332, USA
    Mol Psychiatry 15:115, 204-15. 2010
    ....
  4. ncbi Protease-activated receptor-1 activation of endothelial cells induces protein kinase Calpha-dependent phosphorylation of syntaxin 4 and Munc18c: role in signaling p-selectin expression
    Jian Fu
    Department of Pharmacology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 280:3178-84. 2005
    ..We identified the presence of syntaxin 4, syntaxin 3, and the high affinity syntaxin 4-regulatory protein Munc18c in human lung microvascular endothelial ..
  5. pmc Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2
    M N Jagadish
    CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia
    Biochem J 317:945-54. 1996
    ..It describes the isolation and characterization of cDNA molecules encoding SNAP-25 A and B isoforms, syntaxin 4 and synaptobrevins (also known as vehicle-associated membrane proteins) from two major human insulin-responsive ..
  6. doi Syntaxins 3 and 4 mediate vesicular trafficking of α5β1 and α3β1 integrins and cancer cell migration
    Paul Day
    Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA
    Int J Oncol 39:863-71. 2011
    ..Collectively, these data suggest that syntaxins 3- and 4-dependent integrin trafficking is important in cancer cell migration and survival, and may be a valuable target for cancer therapy...
  7. doi Aberrant localization of fusion receptors involved in regulated exocytosis in salivary glands of Sjögren's syndrome patients is linked to ectopic mucin secretion
    María José Barrera
    Faculty of Medicine, Institute of Biomedical Sciences ICBM, University of Chile, Postal Code 8389100, Santiago, Chile
    J Autoimmun 39:83-92. 2012
    ..of SNARE proteins (membrane fusion receptors) involved in regulated secretion, such as VAMP8, syntaxin 3 (STX3), STX4 and SNAP-23 were altered in salivary glands (SG) from SS-patients...
  8. doi SNARE protein expression and localization in human cytotoxic T lymphocytes
    Varsha Pattu
    Institute of Physiology, Saarland University, Homburg, Germany
    Eur J Immunol 42:470-5. 2012
    ..Vti1b, Stx8 and Stx16 had the highest degrees of co-localization with LGs while Stx3, Stx4, Stx6, Stx7, Stx8, Stx13, Vti1b, VAMP3 and VAMP4 co-localized with CD3...
  9. doi The impact of extracellular syntaxin4 on HaCaT keratinocyte behavior
    Nanako Kadono
    Department of Bioscience, Kwansei Gakuin University, Japan
    Biochem Biophys Res Commun 417:1200-5. 2012
    ..These results revealed, for the first time, the extracellular role of syntaxin4 and shed light on the division of the extracellular effects exerted by epimorphin and syntaxin4 on keratinocyte cornification...
  10. doi Integrating interacting drugs and genetic variations to improve the predictability of warfarin maintenance dose in Chinese patients
    Shi long Zhong
    Medical Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Institute of Clinical Pharmacology, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, People s Republic of China
    Pharmacogenet Genomics 22:176-82. 2012
    ..This study sought to systematically investigate the effects of VKORC1, STX4A, CYP2C9, CYP4F2, CYP3A4, and GGCX gene polymorphisms and interacting drugs on warfarin maintenance dose.
  11. pmc Structure of the Munc18c/Syntaxin4 N-peptide complex defines universal features of the N-peptide binding mode of Sec1/Munc18 proteins
    Shu Hong Hu
    Institute for Molecular Bioscience and Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia
    Proc Natl Acad Sci U S A 104:8773-8. 2007
    ..The mammalian cell surface SM protein Munc18c binds to an N-peptide in Stx4, and this is compatible with its interaction with SNARE complexes...
  12. pmc Asymmetric phospholipid distribution drives in vitro reconstituted SNARE-dependent membrane fusion
    Jerome Vicogne
    Department of Pharmacological Sciences and Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 103:14761-6. 2006
    ..fusion factor attachment receptor) proteins VAMP2 (vesicle-associated membrane protein 2) and syntaxin 4 (Stx4)/SNAP23 (soluble N-ethylmaleimide-sensitive fusion factor attachment protein 23)...
  13. pmc Syntaxins 3 and 4 are concentrated in separate clusters on the plasma membrane before the establishment of cell polarity
    Seng Hui Low
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara 93106 9610, USA
    Mol Biol Cell 17:977-89. 2006
    ..Deletion of the apical targeting signal of syntaxin 3 does not prevent sorting into clusters away from syntaxin 4. Syntaxin 3 and 4 cluster formation depends on different mechanisms because the integrity of syntaxin 3 clusters ..
  14. ncbi Involvement of syntaxin 4 in the transport of membrane-type 1 matrix metalloproteinase to the plasma membrane in human gastric epithelial cells
    Tomohiko Miyata
    Department of Gastroenterology, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 323:118-24. 2004
    ..Immunocytochemistry revealed that transient transfection of AGS cells with dominant-negative mutant syntaxin 4 decreased plasma membrane MT1-MMP expression...
  15. ncbi Identification and characterization of taxilin isoforms
    Satoru Nogami
    Division of Molecular and Cell Biology, Institute for Medical Science, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu machi, Tochigi 321 0293, Japan
    Biochem Biophys Res Commun 319:936-43. 2004
    ..The taxilin family members mutually interacted with the syntaxin family members. These results indicate that there is the taxilin family composed of at least three members in mammals...
  16. ncbi Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis
    Swathi K Rao
    Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06511, USA
    J Biol Chem 279:20471-9. 2004
    ..we show that Ca2+-dependent interaction of the synaptotagmin VII C(2)A domain with SNAP-23 is facilitated by syntaxin 4. Specific interactions also occurred in cell lysates between the plasma membrane t-SNAREs SNAP-23 and syntaxin 4 ..
  17. ncbi Syntaxin 4 and Synip (syntaxin 4 interacting protein) regulate insulin secretion in the pancreatic beta HC-9 cell
    Tsugumichi Saito
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3 39 15, Showa machi, Maebashi shi, Gunma ken, 371 8511, Japan
    J Biol Chem 278:36718-25. 2003
    ..for pancreatic beta cell insulin secretion, we have observed that overexpression of a dominant-interfering syntaxin 4 mutant (syntaxin 4/DeltaTM) attenuated glucose-stimulated insulin secretion in betaHC-9 cells...
  18. pmc A dual mechanism controlling the localization and function of exocytic v-SNAREs
    Sonia Martinez-Arca
    Membrane Traffic and Neuronal Plasticity, Institut National de la Santé et de la Recherche Médicale U536, Institut du Fer a Moulin, 75005 Paris, France
    Proc Natl Acad Sci U S A 100:9011-6. 2003
    ..Moreover, they point to the amino-terminal domains of SNARE proteins as multifunctional modules responsible for the fine tuning of SNARE function...
  19. ncbi Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation
    Charlotte H Widberg
    Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, 2010 Sydney, New South Wales, Australia
    J Biol Chem 278:35093-101. 2003
    ..These data suggest that Tomosyn and Munc18c operate at a similar stage of the Syntaxin4 SNARE assembly cycle, which likely primes Syntaxin4 for entry into the ternary SNARE complex...
  20. ncbi Syntaxin 4 expression affects glucose transporter 8 translocation and embryo survival
    Amanda Hoehn Wyman
    Department of Obstetrics and Gynecology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Endocrinol 17:2096-102. 2003
    ..b>Syntaxin 4 is the t-SNARE critical for insulin-stimulated glucose transporter 4 (GLUT4)-plasma membrane fusion in ..
  21. doi An efficient co-expression and purification system for the complex of Stx4 and C-terminal domain of Synip
    Wei Tian
    College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
    Biochem Biophys Res Commun 371:366-70. 2008
    Synip and Stx4 complex plays a key role in GLUT4 vesicle trafficking and fusion with plasma membrane. The interaction of Synip with Stx4 prevents interaction of VAMP2 located in GLUT4 vesicle with Stx4 in basal state...
  22. pmc A genome-wide scan for common genetic variants with a large influence on warfarin maintenance dose
    Gregory M Cooper
    Department of Genome Sciences, University of Washington, Seattle, WA98195, USA
    Blood 112:1022-7. 2008
    ..Randomized clinical trials that account for these 2 genes should therefore produce results that are definitive and broadly applicable...
  23. doi Involvement of Munc18 isoforms in the regulation of granule exocytosis in neutrophils
    Cristiana Brochetta
    INSERM U699, Paris, F 75018, France
    Biochim Biophys Acta 1783:1781-91. 2008
    ..and Munc18-3 were often located in close proximity to their respective SNARE-binding partners syntaxin 3 and syntaxin 4. Both isoforms were also found in plasma membrane fractions and in the cytosol, where they associate with ..
  24. doi Gene expression profiling reveals renin mRNA overexpression in human hypertensive kidneys and a role for microRNAs
    Francine Z Marques
    Basic and Clinical Genomics Laboratory, School of Medical Sciences and Bosch Institute, Building F13, University of Sydney, Sydney, New South Wales 2006, Australia
    Hypertension 58:1093-8. 2011
    ..We then confirmed expression differences for AIFM1, AMBP, APOE, CD36, EFNB1, NDUFAF1, PRDX5, REN, RENBP, SLC13A1, STX4, and TNNT2 mRNAs and for miRNAs hsa-miR-21, hsa-miR-126, hsa-miR-181a, hsa-miR-196a, hsa-miR-451, hsa-miR-638, and ..
  25. doi VAMP3 regulates podosome organisation in macrophages and together with Stx4/SNAP23 mediates adhesion, cell spreading and persistent migration
    Kelly J Veale
    CHBRI Wound Healing Laboratory, Kids Research Institute at the Children s Hospital at Westmead, Sydney, NSW 2145, Australia
    Exp Cell Res 317:1817-29. 2011
    ..By altering the levels of individual components of the VAMP3/Stx4/SNAP23 complex we show here that this SNARE complex regulates efficient macrophage adhesion, spreading and ..
  26. pmc The role of the recycling endosome in regulating lamellipodia formation and macrophage migration
    Kelly J Veale
    CHBRI Wound Healing Laboratory Kids Research Institute at The Children s Hospital at Westmead Sydney, Australia
    Commun Integr Biol 4:44-7. 2011
    ..In order for this to happen the surface Q-SNARE complex Stx4/SNAP23 translocates to the leading edge where it binds to the R-SNARE VAMP3 on the recycling endosome allowing ..
  27. pmc Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis
    Dean A Wiseman
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Biol Chem 286:16344-54. 2011
    ..stress serving as a mechanistic basis for insulin secretory dysfunction, we identified the t-SNARE protein Syntaxin 4 as a target of modification by S-nitrosylation...
  28. doi The influence of genetic polymorphisms and interacting drugs on initial response to warfarin in Chinese patients with heart valve replacement
    Shi long Zhong
    Guangdong Provincial Cardiovascular Institute, Medical Research Center of Guangdong General Hospital, Guangdong Institute of Clinical Pharmacology, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Guangzhou, GD 510080, People s Republic of China
    Eur J Clin Pharmacol 67:581-90. 2011
    ..This study sought to systematically investigate the effects of genetic polymorphisms of VKORC1, STX4A, CYP2C9, CYP3A4, and GGCX and interacting drugs on the initial responses to warfarin in Chinese patients with ..
  29. doi Role of syntaxin 4 in activity-dependent exocytosis and synaptic plasticity in hippocampal neurons
    Ponnuswamy Mohanasundaram
    Department of Biomedical Sciences, School of Basic Medical Sciences, Bharathidasan University, Tiruchirappalli, India
    Sci Signal 3:jc7. 2010
    ..enriched in the target SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor] syntaxin 4 (Stx4)...
  30. pmc Syntaxin 4 is required for acid sphingomyelinase activity and apoptotic function
    Cristiana Perrotta
    Unit of Clinical Pharmacology, Department of Clinical Sciences, University Hospital Luigi Sacco, Universita degli Studi di Milano, 20157 Milan, Italy
    J Biol Chem 285:40240-51. 2010
    ..from intracellular compartments to the plasma membrane in an exocytic pathway requiring the t-SNARE protein syntaxin 4. Indeed, down-regulation of syntaxin 4 inhibits A-SMase translocation and activation induced by CD95 stimulation...
  31. doi Recycling endosome membrane incorporation into the leading edge regulates lamellipodia formation and macrophage migration
    Kelly J Veale
    CHBRI Wound Healing Laboratory, Kids Research Institute, The Children s Hospital at Westmead, Westmead, Sydney, NSW, Australia
    Traffic 11:1370-9. 2010
    ..the cell surface, as consisting of the R-SNARE VAMP3 on the recycling endosome partnering with the surface Q-SNARE Stx4/SNAP23, which was found to translocate and accumulate on the leading edge of migrating cells...
  32. pmc Reduced plasma membrane expression of dysferlin mutants is attributed to accelerated endocytosis via a syntaxin-4-associated pathway
    Frances J Evesson
    Institute for Neuroscience and Muscle Research, The Children s Hospital at Westmead, Locked Bag 4001, Westmead, New South Wales 2145, Australia
    J Biol Chem 285:28529-39. 2010
    ....
  33. pmc Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines
    Matthew J Kennedy
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Cell 141:524-35. 2010
    ..Exocytosis occurs at microdomains enriched in the plasma membrane t-SNARE syntaxin 4 (Stx4), and disruption of Stx4 impairs both spine exocytosis and long-term potentiation (LTP) at hippocampal ..
  34. doi Regulation of the hDlg/hScrib/Hugl-1 tumour suppressor complex
    Paola Massimi
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I 34012 Trieste, Italy
    Exp Cell Res 314:3306-17. 2008
    ..We also demonstrate an interaction between the three components of the hScrib complex and the tSNARE syntaxin 4, and show that correct localization of the Scrib complex is in part tSNARE dependent...
  35. ncbi Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane
    Akane Imai
    Department of Biochemistry, School of Dentistry at Niigata, The Nippon Dental University, 1 8 Hamaura cho, Niigata 951 8580, Japan
    Arch Oral Biol 48:597-604. 2003
    ..Syntaxins 2 and 3 were localised in the apical plasma membrane (APM), and syntaxin 4 was localised in the plasma membrane. SNAP-23 was localised in the APM and intracellular membrane (ICM)...
  36. pmc A role for Sec1/Munc18 proteins in platelet exocytosis
    Todd D Schraw
    Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA
    Biochem J 374:207-17. 2003
    ..These studies demonstrate a clear role for SM proteins in platelet exocytosis and aggregation and suggest a dominant role for Munc18c in all three granule-release events...
  37. ncbi Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis
    Guangmu Li
    Renal Section, Boston University Medical Center and Department of Medicine, Boston University School of Medicine, Massachusetts 02118 2908, USA
    J Biol Chem 278:19791-7. 2003
    ..Interaction between the syntaxin-1A and H+-ATPase is important in the targeted exocytosis of the proton pump to the apical membrane of intercalated cells...
  38. ncbi Synip: a novel insulin-regulated syntaxin 4-binding protein mediating GLUT4 translocation in adipocytes
    J Min
    Department of Cell Biology, Parke Davis Pharmaceutical Research Division, Warner Lambert Company, Ann Arbor, Michigan 48105, USA
    Mol Cell 3:751-60. 1999
    ..transport and GLUT4 translocation require regulated interactions between the v-SNARE, VAMP2, and the t-SNARE, syntaxin 4. We have isolated a novel syntaxin 4-binding protein, Synip, which specifically interacts with syntaxin 4...
  39. ncbi Three novel proteins of the syntaxin/SNAP-25 family
    M Steegmaier
    Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305 5345, USA
    J Biol Chem 273:34171-9. 1998
    ..SNAP-29 does not contain a predicted membrane anchor characteristic of other SNAREs. In vitro studies established that SNAP-29 is capable of binding to a broad range of syntaxins...
  40. ncbi SNAP-23 in rat kidney: colocalization with aquaporin-2 in collecting duct vesicles
    T Inoue
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol 275:F752-60. 1998
    ..However, localization of SNAP-23 in both intracytoplasmic vesicles and plasma membranes suggests a function different from that originally proposed for SNAP-25 in synaptic vesicle targeting...
  41. ncbi p35, the neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) is degraded by the ubiquitin-proteasome pathway
    G N Patrick
    Howard Hughes Medical Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 273:24057-64. 1998
    ..Furthermore, phosphorylation mutants of p35 also stabilize p35 2- to 3-fold...
  42. ncbi Inhibition of the binding of SNAP-23 to syntaxin 4 by Munc18c
    S Araki
    Second Department of Internal Medicine, Kobe University School of Medicine, Japan
    Biochem Biophys Res Commun 234:257-62. 1997
    ..Expression of SNAP-23, syntaxin 4, and the syntaxin-binding protein Munc 18c in COS cells revealed that Munc18c reduced the amount of SNAP-23 ..
  43. ncbi Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4
    J T Tellam
    Centre for Molecular and Cellular Biology and Department of Physiology and Pharmacology, University of Queensland, St Lucia 4072, Queensland, Australia
    J Biol Chem 272:6179-86. 1997
    ..These data suggest that syntaxin-4/Munc-18c/VAMP-2 may play a role in the docking/fusion of intracellular GLUT-4-containing vesicles with the cell surface in adipocytes...
  44. pmc Identification of SNAP receptors in rat adipose cell membrane fractions and in SNARE complexes co-immunoprecipitated with epitope-tagged N-ethylmaleimide-sensitive fusion protein
    K I Timmers
    Experimental Diabetes, Metabolism and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 320:429-36. 1996
    ..These complexes contain VAMPs 2 and 3 and syntaxin 4, but not syntaxins 2 or 3. Complex formation requires ATP and is disrupted by ATP hydrolysis...
  45. pmc Syntaxin-4 is localized to the apical plasma membrane of rat renal collecting duct cells: possible role in aquaporin-2 trafficking
    B Mandon
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1598, USA
    J Clin Invest 98:906-13. 1996
    ....
  46. ncbi Identification of a novel syntaxin- and synaptobrevin/VAMP-binding protein, SNAP-23, expressed in non-neuronal tissues
    V Ravichandran
    Experimental Immunology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:13300-3. 1996
    ..These findings suggest that SNAP-23 is an essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion in all mammalian cells...
  47. ncbi Isolation and sequence analysis of the human syntaxin-encoding gene
    H Li
    Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702 1201
    Gene 143:303-4. 1994
    ..Here, we describe the nucleotide sequence of the first human STX gene isolated from a placental library. It encodes a 297-amino-acid (aa) protein and is 89% identical to the aa sequence of rat STX 4a...
  48. ncbi Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins
    C Li
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Nature 375:594-9. 1995
    ..The same C2 domains also bind syntaxin as a function of Ca2+ but the Ca(2+)-concentration dependence of Syt I, II and V (> 200 microM) differs from that of Syt III and VII (< 10 microM).(ABSTRACT TRUNCATED AT 250 WORDS)..
  49. ncbi A novel ubiquitous form of Munc-18 interacts with multiple syntaxins. Use of the yeast two-hybrid system to study interactions between proteins involved in membrane traffic
    Y Hata
    Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235, USA
    J Biol Chem 270:13022-8. 1995
    ..produced in COS cells, we show that both forms of Munc-18 bind tightly to syntaxins 1A, 2, and 3 but not to syntaxin 4. In an independent approach to study the binding specificities of Munc-18-1 and Munc-18-2, we used the yeast two-..
  50. pmc Functional and biochemical analysis of the C2 domains of synaptotagmin IV
    D M Thomas
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Mol Biol Cell 10:2285-95. 1999
    ....
  51. ncbi Role of SNAP23 in insulin-induced translocation of GLUT4 in 3T3-L1 adipocytes. Mediation of complex formation between syntaxin4 and VAMP2
    M Kawanishi
    Second Department of Internal Medicine, Kobe University School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    J Biol Chem 275:8240-7. 2000
    ..Together, these results demonstrate that SNAP23 contributes to insulin-dependent trafficking of GLUT4 to the plasma membrane in 3T3-L1 adipocytes by mediating the interaction between t-SNARE (syntaxin4) and v-SNARE (VAMP2)...
  52. ncbi Taxilin; a novel syntaxin-binding protein that is involved in Ca2+-dependent exocytosis in neuroendocrine cells
    Satoru Nogami
    Division of Molecular and Cell Biology, Institute for Medical Science, Mibu machi, Tochigi 321 0293, Japan
    Genes Cells 8:17-28. 2003
    ..However, the molecular mechanism by which the syntaxin family regulates intracellular vesicle transport is not well known...
  53. ncbi Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain
    Steven J Freedman
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    J Biol Chem 278:13462-7. 2003
    ..A comparison of the SNAP-23N structure to other SNARE complex structures reveals how a simple coiled-coil motif can form diverse SNARE complexes...
  54. ncbi Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary
    Faustino Mollinedo
    Centro de Investigacion del Cancer, Instituto de Biologia Molecular y Celular del Cancer, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Spain
    J Immunol 170:1034-42. 2003
    ..Activation of neutrophils led to the interaction of VAMP-2 with the plasma membrane Q-SNARE syntaxin 4, and anti-syntaxin 4 Abs inhibited exocytosis of specific and tertiary granules in electropermeabilized ..
  55. ncbi Endogenous plasma membrane t-SNARE syntaxin 4 is present in rab11 positive endosomal membranes and associates with cortical actin cytoskeleton
    Arja M Band
    Department of Biosciences, Division of Biochemistry, Viikki Biocenter, University of Helsinki, P O Box 56, Viikinkaari 5, 00014, Helsinki, Finland
    FEBS Lett 531:513-9. 2002
    ..b>Syntaxin 4 is a t-SNARE that, according to previous overexpression studies, is predominantly localized at the plasma ..
  56. ncbi Trafficking of green fluorescent protein-tagged SNARE proteins in HSY cells
    Taishin Takuma
    Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061 0293
    J Biochem 132:729-35. 2002
    ..These results suggest that munc18c plays an important role in the trafficking of syntaxin-4 to its proper destination by preventing premature interactions with other proteins, including SNARE proteins...
  57. ncbi Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly
    Suzie J Scales
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5345, USA
    J Biol Chem 277:28271-9. 2002
    ..amisyn, with a tomosyn- and VAMP-like coiled-coil-forming domain that binds specifically to syntaxin 1a and syntaxin 4 both in vitro and in vivo, as assessed by co-immunoprecipitation from rat brain...
  58. ncbi Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion
    Janos Polgar
    Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, MA 02115, USA
    Blood 100:1081-3. 2002
    ..mass spectrometry, we identified VAMP-3 and VAMP-8 as VAMP isoforms coimmunoprecipitated from platelets with syntaxin 4. Immunoblotting experiments confirmed the presence of VAMP-3 and VAMP-8 but not VAMP-1 or VAMP-2 in platelets...
  59. ncbi Regulated transport of the glucose transporter GLUT4
    Nia J Bryant
    Garvan Institute of Medical Research, 384 Victoria Road, Darlinghurst, New South Wales 2010, Australia
    Nat Rev Mol Cell Biol 3:267-77. 2002
    ....
  60. ncbi Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils
    Nasrin Perskvist
    Department of Medical Microbiology, Faculty of Health Science, Linkoping University, SE 581 85 Linkoping, Sweden
    J Cell Sci 115:1321-30. 2002
    ..These results also identified Rab5a as a key regulator of phagolysosome maturation in human neutrophils...
  61. pmc A prototypic platelet septin and its participation in secretion
    Judith Dent
    Division of Experimental Hemostasis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:3064-9. 2002
    ..CDCrel-1 revealed it to be part of a macromolecular complex containing a protein involved in platelet secretion, syntaxin 4. Moreover, CDCrel-1 was localized in situ to areas surrounding platelet-storage granules...
  62. ncbi Expression of eosinophil target SNAREs as potential cognate receptors for vesicle-associated membrane protein-2 in exocytosis
    Michael R Logan
    Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Canada
    J Allergy Clin Immunol 109:299-306. 2002
    ....
  63. ncbi Direct interaction of Rab4 with syntaxin 4
    L Li
    Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Biol Chem 276:5265-73. 2001
    In the present study, we examined the possible interaction between Rab4 and syntaxin 4, both having been implicated in insulin-induced GLUT4 translocation...
  64. ncbi Protein kinase C phosphorylation of syntaxin 4 in thrombin-activated human platelets
    S H Chung
    Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, MA 02115, USA
    J Biol Chem 275:25286-91. 2000
    ..We found that syntaxin 4 was phosphorylated in human platelets treated with a physiologic agent that induces secretion (thrombin) but not ..
  65. ncbi Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment
    F Paumet
    Unite d Immuno Allergie, Institut Pasteur, Paris, France
    J Immunol 164:5850-7. 2000
    ..Within the syntaxin family, syntaxin 4 interacted with SNAP23 and all vesicle-associated membrane proteins (VAMPs) examined, except tetanus neurotoxin ..
  66. ncbi The syntaxin family of vesicular transport receptors
    M K Bennett
    Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical Center, California 94305
    Cell 74:863-73. 1993
    ..These results indicate that the syntaxins are a family of receptors for intracellular transport vesicles and that each target membrane may be identified by a specific member of the syntaxin family...

Research Grants1

  1. Structure and Function of a Platelet SNARE Complex
    Steven Freedman; Fiscal Year: 2003
    ..These studies will be fundamentally important to our understanding of alpha granule secretion in platelets, and will contribute significantly to SNARE protein biology by providing insight into the specificity of membrane fusion. ..