Gene Symbol: ST6GAL1
Description: ST6 beta-galactoside alpha-2,6-sialyltransferase 1
Alias: SIAT1, ST6GalI, ST6N, beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, ST6Gal I, alpha 2,6-ST 1, sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
Species: human
Products:     ST6GAL1

Top Publications

  1. Amano M, Galvan M, He J, Baum L. The ST6Gal I sialyltransferase selectively modifies N-glycans on CD45 to negatively regulate galectin-1-induced CD45 clustering, phosphatase modulation, and T cell death. J Biol Chem. 2003;278:7469-75 pubmed
    ..SAalpha2,6Gal sequences, created by the ST6Gal I enzyme, are present on medullary thymocytes resistant to galectin-1-induced death but not on galectin-1-..
  2. Chiricolo M, Malagolini N, Bonfiglioli S, Dall Olio F. Phenotypic changes induced by expression of beta-galactoside alpha2,6 sialyltransferase I in the human colon cancer cell line SW948. Glycobiology. 2006;16:146-54 pubmed
    ..These data indicate that in the cell line SW948, the effect of ST6Gal.I expression is consistent with the attenuation of the neoplastic phenotype. ..
  3. Vázquez Martin C, Gil Martín E, Fernández Briera A. Elevation of ST6Gal I activity in malignant and transitional tissue in human colorectal cancer. Oncology. 2005;69:436-44 pubmed
    The aim of the present study was to investigate the activity of CMP-NeuAc:Galbeta(1,4)GlcNAc sialyltransferase (ST6Gal I) in colorectal cancer (CRC)...
  4. Sugimoto I, Futakawa S, Oka R, Ogawa K, Marth J, Miyoshi E, et al. Beta-galactoside alpha2,6-sialyltransferase I cleavage by BACE1 enhances the sialylation of soluble glycoproteins. A novel regulatory mechanism for alpha2,6-sialylation. J Biol Chem. 2007;282:34896-903 pubmed
    ..We showed previously that BACE1 cleaves beta-galactoside alpha2,6-sialyltransferase I (ST6Gal I) to initiate its secretion, but it remained unclear how BACE1 affects the cellular level of alpha2,6-sialylation...
  5. Costa Nogueira C, Villar Portela S, Cuevas E, Gil Martín E, Fernández Briera A. Synthesis and expression of CDw75 antigen in human colorectal cancer. BMC Cancer. 2009;9:431 pubmed publisher
    Increased ST6Gal I activity has been associated with the alpha(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC)...
  6. Liu Z, Swindall A, Kesterson R, Schoeb T, Bullard D, Bellis S. ST6Gal-I regulates macrophage apoptosis via ?2-6 sialylation of the TNFR1 death receptor. J Biol Chem. 2011;286:39654-62 pubmed publisher
  7. Swindall A, Londoño Joshi A, Schultz M, Fineberg N, Buchsbaum D, Bellis S. ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines. Cancer Res. 2013;73:2368-78 pubmed publisher
    ..Collectively, our results suggest that ST6Gal-I promotes tumorigenesis and may serve as a regulator of the stem cell phenotype in both normal and cancer cell populations. ..
  8. Gretschel S, Haensch W, Schlag P, Kemmner W. Clinical relevance of sialyltransferases ST6GAL-I and ST3GAL-III in gastric cancer. Oncology. 2003;65:139-45 pubmed
  9. Kitazume S, Oka R, Ogawa K, Futakawa S, Hagiwara Y, Takikawa H, et al. Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo. Glycobiology. 2009;19:479-87 pubmed publisher
    Beta-galactoside alpha2,6-sialyltransferase (ST6Gal I), which is highly expressed in the liver, is mainly cleaved by Alzheimer's beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and secreted into the serum...

More Information

Publications152 found, 100 shown here

  1. Lee M, Park J, Lee Y. Adhesion of ST6Gal I-mediated human colon cancer cells to fibronectin contributes to cell survival by integrin beta1-mediated paxillin and AKT activation. Oncol Rep. 2010;23:757-61 pubmed
    ..that ionizing radiation (IR) of cells increased the expression of beta-galactoside alpha-(2,6)-sialyltransferase (ST6Gal I) and the level of glycoprotein sialylation, especially for the key adhesion molecule integrin beta1...
  2. Jun L, Yuanshu W, Yanying X, Zhongfa X, Jian Y, Fengling W, et al. Altered mRNA expressions of sialyltransferases in human gastric cancer tissues. Med Oncol. 2012;29:84-90 pubmed publisher
    ..05) unlike that of ST6Gal I. These results indicated that gastric cancer tissues expressed high levels of ? 2,3-linked sialic acid residues, ..
  3. Swindall A, Bellis S. Sialylation of the Fas death receptor by ST6Gal-I provides protection against Fas-mediated apoptosis in colon carcinoma cells. J Biol Chem. 2011;286:22982-90 pubmed publisher
    ..This finding establishes a new paradigm by which death receptor function is impaired for the self-protection of tumors against apoptosis. ..
  4. Antony P, Rose M, Heidenreich A, Knüchel R, Gaisa N, Dahl E. Epigenetic inactivation of ST6GAL1 in human bladder cancer. BMC Cancer. 2014;14:901 pubmed publisher
    ..Accumulating evidence shows that ST6GAL1, an enzyme that catalyzes the transfer of sialic acid onto galactose-containing substrates, is aberrantly ..
  5. Matsumoto S, Chong Y, Kang D, Ikematsu H. High genetic stability in MDCK-SIAT1 passaged human influenza viruses. J Infect Chemother. 2019;25:222-224 pubmed publisher
    ..MDCK-SIAT1 cells, modified derivatives with an increased expression of α2,6-linked sialic acid receptors are increasingly ..
  6. Dall Olio F. Glycobiology of Aging. Subcell Biochem. 2018;90:505-526 pubmed publisher
    ..strong association with aging has been documented also for the plasmatic forms of glycosyltrasferases B4GALT1 and ST6GAL1, although their role in the extracellular glycosylation of antibodies does not appear likely...
  7. Zhang Q, Higginbotham J, Jeppesen D, Yang Y, Li W, McKinley E, et al. Transfer of Functional Cargo in Exomeres. Cell Rep. 2019;27:940-954.e6 pubmed publisher
    ..These findings underscore the heterogeneity of nanoparticles and should accelerate advances in determining the composition and biological functions of exomeres. ..
  8. Kazuma S, Tokinaga Y, Kimizuka M, Azumaguchi R, Hamada K, Yamakage M. Sevoflurane Promotes Regeneration of the Endothelial Glycocalyx by Upregulating Sialyltransferase. J Surg Res. 2019;241:40-47 pubmed publisher
    ..Sevoflurane exerts regenerative effects on endothelium-dependent vasodilation and the endothelial glycocalyx following oxidative stress in the rat aorta. ..
  9. Alam S, Anugraham M, Huang Y, Kohler R, Hettich T, Winkelbach K, et al. Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells. Sci Rep. 2017;7:45367 pubmed publisher
    ..known α2-6 sialyltransferase-encoding genes and showed that the loss of α2-6 sialylation is due to silencing of ST6GAL1 expression in ∆B3GNT5 cells...
  10. Fage C, Abed Y, Checkmahomed L, Venable M, Boivin G. In Vitro Properties and Virulence of Contemporary Recombinant Influenza B Viruses Harboring Mutations of Cross-Resistance to Neuraminidase Inhibitors. Viruses. 2018;11: pubmed publisher
    ..Their replicative capacity and virulence were evaluated in ST6GalI-MDCK cells and BALB/c mice, respectively...
  11. Holdbrooks A, Britain C, Bellis S. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor. J Biol Chem. 2018;293:1610-1622 pubmed publisher
    ..These collective findings point to a novel glycosylation-dependent mechanism that regulates the cellular response to TNF and may promote cancer cell survival within TNF-rich tumor microenvironments. ..
  12. Luley Goedl C, Schmoelzer K, Thomann M, Malik S, Greif M, Ribitsch D, et al. Two N-terminally truncated variants of human β-galactoside α2,6 sialyltransferase I with distinct properties for in vitro protein glycosylation. Glycobiology. 2016;26:1097-1106 pubmed publisher
    ..While the enzyme Δ89ST6Gal-I exhibited both ST (di-sialylation) and sialidase activity on a monoclonal antibody, the enzyme Δ108ST6Gal-I showed only ST activity with specificity for mono-sialylation. ..
  13. Shabana -, Shahid S, Hasnain S. Use of a gene score of multiple low-modest effect size variants can predict the risk of obesity better than the individual SNPs. Lipids Health Dis. 2018;17:155 pubmed publisher Growth Factor Receptor Bound Protein 14 (GRB14), rs16861329 in Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), rs1802295 in Vacuolar protein sorting-associated protein 26A (VPS26A), rs7178572 in high mobility group 20A (..
  14. Shi M, Ouyang Y, Yang M, Yang M, Zhang X, Huang W, et al. IgA Nephropathy Susceptibility Loci and Disease Progression. Clin J Am Soc Nephrol. 2018;13:1330-1338 pubmed publisher
    ..A four-SNP model, rs11150612 (ITGAM-ITGAX), rs7634389 (ST6GAL1), rs2412971 (HORMAD2), and rs2856717 (HLA-DQ/DR), was selected as the best predictive model...
  15. Imamaki R, Ogawa K, Kizuka Y, Komi Y, Kojima S, Kotani N, et al. Glycosylation controls cooperative PECAM-VEGFR2-β3 integrin functions at the endothelial surface for tumor angiogenesis. Oncogene. 2018;37:4287-4299 pubmed publisher
    ..Here we demonstrate a glycosylation-dependent regulatory mechanism for tumor angiogenesis. St6gal1-/- mice, lacking the α2,6-sialylation enzyme, were shown to exhibit impaired tumor angiogenesis ..
  16. Lin N, Mascarenhas J, Sealover N, George H, Brooks J, Kayser K, et al. Chinese hamster ovary (CHO) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialyltransferase expression. Biotechnol Prog. 2015;31:334-46 pubmed publisher Chinese hamster overy (CHO) only have α2,3-linkage due to the absence of α2,6-sialyltransferase (St6gal1) expression...
  17. Ann J, Abed Y, Beaulieu E, Bouhy X, Joly M, Dubé K, et al. Impact of a large deletion in the neuraminidase protein identified in a laninamivir-selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness in vitro and in ferrets. Influenza Other Respir Viruses. 2016;10:122-6 pubmed publisher
    ..LRVp9 and the wild-type (WT) virus showed comparable replication kinetics in MDCK-ST6GalI cells...
  18. Nagao M, Tanabe N, Manaka S, Naito M, Sekino J, Takayama T, et al. LIPUS suppressed LPS-induced IL-1α through the inhibition of NF-κB nuclear translocation via AT1-PLCβ pathway in MC3T3-E1 cells. J Cell Physiol. 2017;232:3337-3346 pubmed publisher
    ..We also investigate to clarify the pathway of LIPUS, we transfected siRNA silencing AT1 (siAT1) in MC3T3-E1. LIPUS inhibited mRNA and protein expression of LPS-induced IL-1α...
  19. Han Y, Liu Y, Fu X, Zhang Q, Huang H, Zhang C, et al. miR-9 inhibits the metastatic ability of hepatocellular carcinoma via targeting beta galactoside alpha-2,6-sialyltransferase 1. J Physiol Biochem. 2018;74:491-501 pubmed publisher
    ..Additionally, β-galactoside α-2,6-sialyltransferase 1 (St6gal1) expression level is associated negatively with miR-9 and positively with metastatic potential...
  20. Sanpui P, Zheng X, Loeb J, Bisesi J, Khan I, Afrooz A, et al. Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells. Part Fibre Toxicol. 2014;11:66 pubmed publisher
    ..of targets involved in inflammation and anti-viral activity (INFβ1, IL-8, RANTES/CCL5, IFIT2, IFIT3, ST3GAL4, ST6GAL1, IL-10), localized sialic acid receptors, and assessed mitochondrial function...
  21. Bassagañas S, Allende H, Cobler L, Ortiz M, Llop E, de Bolós C, et al. Inflammatory cytokines regulate the expression of glycosyltransferases involved in the biosynthesis of tumor-associated sialylated glycans in pancreatic cancer cell lines. Cytokine. 2015;75:197-206 pubmed publisher
    ..mRNA levels of ST3GAL3-4 and FUT5-7, which codify for ST and FucT enzymes related to SLe(x) biosynthesis, and of ST6GAL1. IL-6 and TNFα treatments increased the levels of SLe(x) and Le(y) antigens in MDPanc-3 cells and, similarly, ..
  22. Yuan Q, Chen X, Han Y, Lei T, Wu Q, Yu X, et al. Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway. Int J Cancer. 2018;143:2319-2330 pubmed publisher
    ..mRNA levels of ST3GAL1, ST6GALNAC3 and ST8SIA6 were remarkably reduced in lung cancer tissues and cells, whereas ST6GAL1 level significantly increased...
  23. Prestegard J, Agard D, Moremen K, Lavery L, Morris L, Pederson K. Sparse labeling of proteins: structural characterization from long range constraints. J Magn Reson. 2014;241:32-40 pubmed publisher a glycosylated protein where resolution is achieved by single amino acid labeling (the sialyltransferase, ST6Gal1)...
  24. Gong M, Garige M, Hirsch K, Lakshman M. Liver Galbeta1,4GlcNAc alpha2,6-sialyltransferase is down-regulated in human alcoholics: possible cause for the appearance of asialoconjugates. Metabolism. 2007;56:1241-7 pubmed
    Galbetal,4GlcNAc alpha2,6-sialyltransferase (ST6GalI) mediates the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment...
  25. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
    ..This study also confirms aneuploidy in LP cells, provides antigens that may be helpful in distinguishing NLPHL from CHL, and suggests that T cells interact less avidly with LP cells than with Hodgkin and Reed-Sternberg cells. ..
  26. Dall Olio F, Chiricolo M, D Errico A, Gruppioni E, Altimari A, Fiorentino M, et al. Expression of beta-galactoside alpha2,6 sialyltransferase and of alpha2,6-sialylated glycoconjugates in normal human liver, hepatocarcinoma, and cirrhosis. Glycobiology. 2004;14:39-49 pubmed
    ..Neither ST6Gal.I expression nor alpha2,6-sialylation are altered in cirrhosis. These data indicate that neoplastic transformation but not cirrhosis can alter the process of alpha2,6-sialylation of liver glycoproteins. ..
  27. Engdahl C, Bondt A, Harre U, Raufer J, Pfeifle R, Camponeschi A, et al. Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis: a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women. Arthritis Res Ther. 2018;20:84 pubmed publisher
    ..Furthermore, effects of E2 on the expression of the sialylation enzyme β-galactoside α2,6-sialyltransferase 1 (St6Gal1) were studied in mouse and human antibody-producing cells...
  28. Shin B, Kress R, Kramer P, Darley Usmar V, Bellis S, Harrington L. Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation. J Exp Med. 2018;215:1803-1812 pubmed publisher
    ..Our findings indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties. ..
  29. Priyambada S, Misaki R, Okamoto T, Ohashi T, Ueda K, Matsuura Y, et al. St6gal1 knockdown alters HBV life cycle in HepAD38 cells. Biochem Biophys Res Commun. 2018;503:1841-1847 pubmed publisher
    ..we found that HBV replication in HepAD38 altered cell-surface sialylated N-glycan through the upregulation of St6gal1, Mgat2, and Mgat4a expression. Here we studied the effects of knocking them down on HBV replication in HepAD38...
  30. Shabana -, Ullah Shahid S, Wah Li K, Acharya J, Cooper J, Hasnain S, et al. Effect of six type II diabetes susceptibility loci and an FTO variant on obesity in Pakistani subjects. Eur J Hum Genet. 2016;24:903-10 pubmed publisher
    ..of the current study was to analyze the effect of six type II diabetes GWAS loci rs3923113 (GRB14), rs16861329 (ST6GAL1), rs1802295 (VPS26A), rs7178572 (HMG20A), rs2028299 (AP3S2) and rs4812829 (HNF4A), and an FTO polymorphism (..
  31. Britain C, Dorsett K, Bellis S. The Glycosyltransferase ST6Gal-I Protects Tumor Cells against Serum Growth Factor Withdrawal by Enhancing Survival Signaling and Proliferative Potential. J Biol Chem. 2017;292:4663-4673 pubmed publisher
    ..Collectively, these results implicate a functional role for ST6Gal-I in fostering tumor cell survival within the serum-depleted tumor microenvironment. ..
  32. Little K, Leang S, Butler J, Baas C, Harrower B, Mosse J, et al. Zanamivir-resistant influenza viruses with Q136K or Q136R neuraminidase residue mutations can arise during MDCK cell culture creating challenges for antiviral susceptibility monitoring. Euro Surveill. 2015;20: pubmed publisher
    ..preferentially selected in Madin-Darby canine kidney epithelial (MDCK) cells, but were less well supported in MDCK-SIAT1 cells and embryonated eggs...
  33. Marx N, Grünwald Gruber C, Bydlinski N, Dhiman H, Ngoc Nguyen L, Klanert G, et al. CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells. Biotechnol J. 2018;13:e1700217 pubmed publisher
    ..The present, but silent beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) gene is activated by targeting the catalytic domain (CD) of Ten-Eleven Translocation methylcytosine dioxygenase ..
  34. Mo D, Costa S, Ihrke G, Youker R, Pastor Soler N, Hughey R, et al. Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism. Mol Biol Cell. 2012;23:3636-46 pubmed publisher
    ..They raise the intriguing possibility that changes in glycosyltransferase expression patterns and/or galectin-9 distribution may acutely modulate endolyn trafficking in the kidney. ..
  35. Ip C, Yung S, Chan T, Tsao S, Wong A. p70 S6 kinase drives ovarian cancer metastasis through multicellular spheroid-peritoneum interaction and P-cadherin/b1 integrin signaling activation. Oncotarget. 2014;5:9133-49 pubmed
    ..These findings establish a new role for p70S6K in tumor spheroid-mesothelium communication in ovarian cancer and provide a preclinical rationale for targeting p70S6K as a new avenue for microenvironment-based therapeutic strategy. ..
  36. Daly A, Donaldson P, Bhatnagar P, Shen Y, Pe er I, Floratos A, et al. HLA-B*5701 genotype is a major determinant of drug-induced liver injury due to flucloxacillin. Nat Genet. 2009;41:816-9 pubmed publisher
    ..The association was replicated in a second cohort of 23 cases. In HLA-B*5701 carrier cases, rs10937275 in ST6GAL1 on chromosome 3 also showed genome-wide significance (OR = 4.1, P = 1.4 x 10(-8))...
  37. Li M, Foo J, Wang J, Low H, Tang X, Toh K, et al. Identification of new susceptibility loci for IgA nephropathy in Han Chinese. Nat Commun. 2015;6:7270 pubmed publisher
    ..Here, we show novel associations at ST6GAL1 on 3q27.3 (rs7634389, odds ratio (OR)=1.13, P=7.27 × 10(-10)), ACCS on 11p11.2 (rs2074038, OR=1.14, P=3...
  38. Aas Eng D, Asheim H, Deggerdal A, Smeland E, Funderud S. Characterization of a promoter region supporting transcription of a novel human beta-galactoside alpha-2,6-sialyltransferase transcript in HepG2 cells. Biochim Biophys Acta. 1995;1261:166-9 pubmed
    ..The expression of this transcript is controlled by a promoter region which efficiently supports transcription in HepG2 cells, and which harbours putative binding sites for liver-enriched and acute phase inducible transcription factors. ..
  39. Luley Goedl C, Czabany T, Longus K, Schmölzer K, Zitzenbacher S, Ribitsch D, et al. Combining expression and process engineering for high-quality production of human sialyltransferase in Pichia pastoris. J Biotechnol. 2016;235:54-60 pubmed publisher
    ..The sialyltransferase was recovered from the bioreactor culture in a yield of 70% using a single step of anion-exchange chromatography. Its specific activity was 0.05units/mg protein. ..
  40. Cui H, Yang S, Jiang Y, Li C, Zhao Y, Shi Y, et al. The glycosyltransferase ST6Gal-I is enriched in cancer stem-like cells in colorectal carcinoma and contributes to their chemo-resistance. Clin Transl Oncol. 2018;20:1175-1184 pubmed publisher
    ..ST6Gal-I may be highly expressed in the cancer stem-like cells in CRC and enhances cancer cell resistance to chemotherapy. ..
  41. Woodard Grice A, McBrayer A, Wakefield J, Zhuo Y, Bellis S. Proteolytic shedding of ST6Gal-I by BACE1 regulates the glycosylation and function of alpha4beta1 integrins. J Biol Chem. 2008;283:26364-73 pubmed publisher
    ..Collectively, these results describe a novel mechanism for alpha4beta1 regulation and further suggest an unanticipated role for BACE1 in macrophage function. ..
  42. Nio Kobayashi J. Tissue- and cell-specific localization of galectins, ?-galactose-binding animal lectins, and their potential functions in health and disease. Anat Sci Int. 2017;92:25-36 pubmed
    ..The ligand glycoconjugate structures mediated by glycosyltransferases including MGAT5, ST6GAL1, and C2GnT are important for revealing the functions of galectins in healthy and disease states.
  43. Zhang J, Liu Y, Deng X, Chen L, Yang X, Yu C. ST6GAL1 negatively regulates monocyte transendothelial migration and atherosclerosis development. Biochem Biophys Res Commun. 2018;500:249-255 pubmed publisher
    ..a critical step in the initial stage of atherosclerosis, in which the involvement of ?-2,6 sialyltransferase 1 (ST6GAL1) has been confirmed by increasing evidence...
  44. Tu V, Abed Y, Fage C, Baz M, Boivin G. Impact of R152K and R368K neuraminidase catalytic substitutions on in vitro properties and virulence of recombinant A(H1N1)pdm09 viruses. Antiviral Res. 2018;154:110-115 pubmed publisher
    ..The R152K and R368K variants grew at comparable or even higher titers than the WT in both MDCK and ST6GalI-MDCK cells...
  45. Liu H, Wu Q, Liu W, Liu Y, Fu Q, Zhang W, et al. ST6Gal-I predicts postoperative clinical outcome for patients with localized clear-cell renal cell carcinoma. Asian Pac J Cancer Prev. 2014;15:10217-23 pubmed
    ..High ST6Gal-I expression is a potential independent adverse predictor of survival and recurrence in ccRCC patients, and the prognostic value is most prominent in those with low and intermediate-risk disease defined by the UISS score. ..
  46. Jones M. IgG and leukocytes: Targets of immunomodulatory α2,6 sialic acids. Cell Immunol. 2018;333:58-64 pubmed publisher
    b>ST6Gal1 is a critical sialyltransferase enzyme that controls the addition of α2,6-linked sialic acids to the termini of glycans...
  47. Kooner J, Saleheen D, Sim X, Sehmi J, Zhang W, Frossard P, et al. Genome-wide association study in individuals of South Asian ancestry identifies six new type 2 diabetes susceptibility loci. Nat Genet. 2011;43:984-9 pubmed publisher
    ..In the combined analysis, we identified common genetic variants at six loci (GRB14, ST6GAL1, VPS26A, HMG20A, AP3S2 and HNF4A) newly associated with T2D (P = 4.1 × 10(-8) to P = 1.9 × 10(-11))...
  48. Minami A, Shimono Y, Mizutani K, Nobutani K, Momose K, Azuma T, et al. Reduction of the ST6 ?-galactosamide ?-2,6-sialyltransferase 1 (ST6GAL1)-catalyzed sialylation of nectin-like molecule 2/cell adhesion molecule 1 and enhancement of ErbB2/ErbB3 signaling by microRNA-199a. J Biol Chem. 2013;288:11845-53 pubmed publisher
    ..Necl-2 was at least sialylated by the sialyltransferase ST6 ?-galactosamide ?-2,6-sialyltransferase 1 (ST6GAL1). miR-199a targeted ST6GAL1 and reduced both the sialylation and the protein level of Necl-2...
  49. Hsieh C, Shyr Y, Liao W, Chen T, Wang S, Lu P, et al. Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructoseresponsive manner promotes pancreatic cancer metastasis. Oncotarget. 2017;8:7691-7709 pubmed publisher
    ..and elevating N-acetylmannosamine levels that upregulated β-galactoside α2,6-sialyltransferase 1 (ST6Gal1), thereby promoting distant metastasis...
  50. Gross F, Bai Y, Jefferson S, Holiday C, Levine M. Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells. J Vis Exp. 2017;: pubmed publisher
    ..2a and 3C.3a A(H3N2) influenza viruses have acquired altered receptor binding specificity. The MDCK-SIAT1 cell line with increased α-2,6 sialic galactose moieties on the surface has proven to provide improved ..
  51. Chang T, Chin Y, Nana A, Wang S, Liao Y, Chen Y, et al. Enhancement by Nano-Diamino-Tetrac of Antiproliferative Action of Gefitinib on Colorectal Cancer Cells: Mediation by EGFR Sialylation and PI3K Activation. Horm Cancer. 2018;9:420-432 pubmed publisher
    ..Galactoside α-2,6-sialyltransferase 1 (ST6Gal1) catalyzes EGFR sialylation that is associated with gefitinib resistance in colorectal cancers, and this was also ..
  52. Munkley J, Vodák D, Livermore K, James K, Wilson B, Knight B, et al. Glycosylation is an Androgen-Regulated Process Essential for Prostate Cancer Cell Viability. EBioMedicine. 2016;8:103-116 pubmed publisher
    ..31 reciprocally-regulated glycosylation enzymes, a set of 8 (GALNT7, ST6GalNAc1, GCNT1, UAP1, PGM3, CSGALNACT1, ST6GAL1 and EDEM3) were significantly up-regulated in clinical prostate carcinoma...
  53. Jones R, Dorsett K, Hjelmeland A, Bellis S. The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1α signaling. J Biol Chem. 2018;293:5659-5667 pubmed publisher
    ..Taken together, these findings unravel a glycosylation-dependent mechanism that facilitates tumor cell adaptation to a hypoxic milieu. ..
  54. Takahashi M, Kizuka Y, Ohtsubo K, Gu J, Taniguchi N. Disease-associated glycans on cell surface proteins. Mol Aspects Med. 2016;51:56-70 pubmed publisher
    ..N-acetylglucosaminyltransferase IV (GnT-IV), N-acetylglucosaminyltransferase V (GnT-V), ?2,6 sialyltransferase 1 (ST6GAL1), and ?1,6 fucosyltransferase (Fut8), which are typical N-glycan branching enzymes and play pivotal roles in ..
  55. Schoenhofen I, Young N, Gilbert M. Biosynthesis of Legionaminic Acid and Its Incorporation Into Glycoconjugates. Methods Enzymol. 2017;597:187-207 pubmed publisher
    ..The two mammalian sialyltransferases that have been tested so far (porcine ST3Gal1 and human ST6Gal1) were found to be more efficient than the bacterial sialyltransferases for the modification of glycoproteins...
  56. Yu S, Zhao P, Sun T, Gao Z, Moremen K, Boons G, et al. Selective Exo-Enzymatic Labeling Detects Increased Cell Surface Sialoglycoprotein Expression upon Megakaryocytic Differentiation. J Biol Chem. 2016;291:3982-9 pubmed publisher undifferentiated and differentiated human erythroleukemia cells (HEL) using SEEL using the sialyltransferases ST6Gal1 and ST3Gal1, which label N- and O-glycans, respectively...
  57. Zhao Y, Wei A, Chen X, Wang L, Zhang H, Yu X, et al. ?2,6-Sialylation mediates hepatocellular carcinoma growth in vitro and in vivo by targeting the Wnt/?-catenin pathway. Oncogenesis. 2017;6:e343 pubmed publisher
  58. Ogata S, Shimizu C, Franco A, Touma R, Kanegaye J, Choudhury B, et al. Treatment response in Kawasaki disease is associated with sialylation levels of endogenous but not therapeutic intravenous immunoglobulin G. PLoS ONE. 2013;8:e81448 pubmed publisher
    ..Rather, lower sialylation of endogenous IgG and lower blood levels of ST6GALI mRNA and ST6Gal-I enzyme predict therapy resistance...
  59. Villar Portela S, Muinelo Romay L, Cuevas E, Gil Martín E, Fernández Briera A. Disease-free survival of colorectal cancer patients in relation to CDw75 antigen expression. Pathobiology. 2011;78:201-9 pubmed publisher
    ..CDw75 expression in CRC is not a prognostic factor for predicting disease-free survival. Nevertheless, CDw75 expression may be a good marker of tumour progression and of the malignant potential of CRC. ..
  60. Kaburagi T, Kizuka Y, Kitazume S, Taniguchi N. The Inhibitory Role of α2,6-Sialylation in Adipogenesis. J Biol Chem. 2017;292:2278-2286 pubmed publisher
    ..We found that a gene encoding β-galactoside α2,6-sialyltransferase-1 (St6gal1), a key enzyme responsible for the biosynthesis of α2,6-linked sialic acid in N-linked glycans, was ..
  61. Meng Q, Ren C, Wang L, Zhao Y, Wang S. Knockdown of ST6Gal-I inhibits the growth and invasion of osteosarcoma MG-63 cells. Biomed Pharmacother. 2015;72:172-8 pubmed publisher
    ..Together, our results suggest a role for ST6Gal-I to promote the growth and invasion of osteosarcoma cells through modulation of EMT-related molecules, and might be a promising marker for the prognosis and therapy of osteosarcoma. ..
  62. Maslak H, Kostiuk O, Minchenko D, Brazaluk O, Shevtsova A, Minchenko O. [Glycoprotein sialylation and NEU1 and ST6GAL1 expressions in erythremia]. Fiziol Zh. 2014;60:14-22 pubmed
    ..of lymphocyte surface-associated sialylglycans and the expression of neuraminidase NEU1 and sialyltransferase ST6GAL1 genes in lymphocytes in erythremia patients and healthy donors as well as the levels of sialic acids in plasma ..
  63. Lu J, Gu J. Significance of β-Galactoside α2,6 Sialyltranferase 1 in Cancers. Molecules. 2015;20:7509-27 pubmed publisher
    ..However, the increased α2,6 sialylation catalyzed by β-galactoside α2,6 sialyltranferase 1 (ST6Gal I) is frequently observed in many types of the cancers...
  64. Qian X, Sands J, Song X, Chen G. Modulation of kidney urea transporter UT-A3 activity by alpha2,6-sialylation. Pflugers Arch. 2016;468:1161-70 pubmed publisher
    ..We explored the enzymes responsible for directing UT-A3 sialylation. Sialyltransferase ST6GalI, but not ST3GalIV, catabolizes UT-A3 α2,6-sialylation...
  65. Munkley J. Glycosylation is a global target for androgen control in prostate cancer cells. Endocr Relat Cancer. 2017;24:R49-R64 pubmed publisher
    ..cells and further defined a set of 8 glycosylation enzymes (GALNT7, ST6GalNAc1, GCNT1, UAP1, PGM3, CSGALNACT1, ST6GAL1 and EDEM3), which are also significantly upregulated in prostate cancer tissue...
  66. Inafuku S, Noda K, Amano M, Ohashi T, Yoshizawa C, Saito W, et al. Alteration of N-Glycan Profiles in Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2015;56:5316-22 pubmed publisher
    ..4 ± 5.8 pg/mg; ST3GAL4, 3.8 ± 0.3 pg/mg). Our data demonstrate distinct changes in the N-glycan profile and an increase in sialylated N-glycans in eyes with PDR. ..
  67. Schultz M, Holdbrooks A, Chakraborty A, Grizzle W, Landen C, Buchsbaum D, et al. The Tumor-Associated Glycosyltransferase ST6Gal-I Regulates Stem Cell Transcription Factors and Confers a Cancer Stem Cell Phenotype. Cancer Res. 2016;76:3978-88 pubmed publisher
    ..Collectively, this work highlighted a previously unrecognized role for a specific glycosyltransferase in driving a CSC state. Cancer Res; 76(13); 3978-88. ©2016 AACR. ..
  68. Priyambada S, Misaki R, Okamoto T, Okamoto Y, Ohashi T, Ueda K, et al. Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus. Virus Res. 2017;238:101-109 pubmed publisher
    ..Among the glycosyltransferases upregulated by the expression of HBV were GnT-II, GnT-IVa, ST6Gal1, and GnT-V, whereas GnT-I, GnT-III, β4GalT1, and FUT8 were downregulated...
  69. Shi G, Du Y, Li Y, An Y, He Z, Lin Y, et al. Cell Recognition Molecule L1 Regulates Cell Surface Glycosylation to Modulate Cell Survival and Migration. Int J Med Sci. 2017;14:1276-1283 pubmed publisher
    ..Activated L1 upregulated the protein expressions of ST6Gal1 (?-galactoside ?-2,6-sialyltransferase 1) and FUT9 (Fucosyltransferase 9) in CHO cells...
  70. Suzuki O, Abe M, Hashimoto Y. Sialylation by β-galactoside α-2,6-sialyltransferase and N-glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma. Int J Oncol. 2015;46:973-80 pubmed publisher
    ..The knockdown of β‑galactoside α‑2,6‑sialyltrans-ferase (ST6Gal1) by siRNA showed inhibition of ST6Gal1 expression in the cytoplasm of H‑ALCL cells on immunohistochemical ..
  71. Hashiba K, Nio Kobayashi J, Sano M, Maeda M, Kimura Y, Yamamoto Y, et al. Possible Contribution of Alpha2,6-Sialylation to Luteolysis in Cows by Inhibiting the Luteotropic Effects of Galectin-1. Biol Reprod. 2016;95:17 pubmed publisher
    ..The overall findings suggest that alpha2,6-sialylation stimulated by PGF2alpha contributes to luteolysis by inhibiting the luteotropic effects of galectin-1 in bovine CL. ..
  72. Kanthimathi S, Chidambaram M, Bodhini D, Liju S, Bhavatharini A, Uma R, et al. Association of recently identified type 2 diabetes gene variants with Gestational Diabetes in Asian Indian population. Mol Genet Genomics. 2017;292:585-591 pubmed publisher
    ..rs12056034), CDKN2A/B (rs7020996), GRB14 (rs3923113), HHEX (rs7923837), HMG20A (rs7178572), HNF4A (rs4812829), ST6GAL1 (rs16861329) and VPS26A (rs1802295) were genotyped using the MassARRAY system...
  73. Lo N, Lau J. Novel heterogeneity exists in the 5'-untranslated region of the beta-galactoside alpha 2,6-sialytransferase mRNAs in the human B-lymphoblastoid cell line, louckes. Biochem Biophys Res Commun. 1996;228:380-5 pubmed
    ..mRNA isoforms are generated from SIAT1, the gene encoding the beta-galactoside alpha 2,6-sialyltransferase (ST6Gal I, SiaT-1, ST6N, alpha 2,6ST)...
  74. Watson D, Wakarchuk W, Gervais C, Durocher Y, Robotham A, Fernandes S, et al. Preparation of legionaminic acid analogs of sialo-glycoconjugates by means of mammalian sialyltransferases. Glycoconj J. 2015;32:729-34 pubmed publisher
    ..and α1-antitrypsin, by means of two mammalian sialyltransferases, namely porcine ST3Gal1 and human ST6Gal1. The Leg5Ac7Ac form of GD1a was not recognized by the myelin-associated glycoprotein (MAG, Siglec-4), confirming ..
  75. Jones M, Oswald D, Joshi S, Whiteheart S, Orlando R, Cobb B. B-cell-independent sialylation of IgG. Proc Natl Acad Sci U S A. 2016;113:7207-12 pubmed publisher
    ..terminal ?2,6-linked sialic acids added to conserved N-glycans within the Fc domain by the sialyltransferase ST6Gal1 accounts for the anti-inflammatory effects of large-dose i.v. Ig (IVIg) in autoimmunity...
  76. Capicciotti C, Zong C, Sheikh M, Sun T, Wells L, Boons G. Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative. J Am Chem Soc. 2017;139:13342-13348 pubmed publisher
    ..It is shown that recombinant ST6GAL1 can readily transfer the modified sialic acid to N-glycans of glycoprotein acceptors of living cells resulting in ..
  77. Breen K, Georgopoulou N. The role of protein phosphorylation in alpha2,6(N)-sialyltransferase activity. Biochem Biophys Res Commun. 2003;309:32-5 pubmed
    ..have determined the role of specific kinase enzymes in the expression and catalytic activity of the alpha2,6 STN (ST6N) isozyme. The catalytic activity was moderately decreased following the inhibition of GSK3beta with LiCl...
  78. Vojta A, Samaržija I, Bočkor L, Zoldoš V. Glyco-genes change expression in cancer through aberrant methylation. Biochim Biophys Acta. 2016;1860:1776-85 pubmed publisher
    ..Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc ..
  79. Hanasaki K, Varki A, Stamenkovic I, Bevilacqua M. Cytokine-induced beta-galactoside alpha-2,6-sialyltransferase in human endothelial cells mediates alpha 2,6-sialylation of adhesion molecules and CD22 ligands. J Biol Chem. 1994;269:10637-43 pubmed
    ..Thus, activation of endothelial cells during inflammatory and immunological processes may induce alpha 2-6STN, which can participate in sialylation of other activation-dependent molecules. ..
  80. Dougher C, Buffone A, Nemeth M, Nasirikenari M, Irons E, Bogner P, et al. The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis. J Leukoc Biol. 2017;102:507-516 pubmed publisher
    ..The data confirm that circulatory ST6Gal-1 is a negative systemic regulator of granulopoiesis and moreover suggest a clinical potential to limit the number of inflammatory cells by manipulating blood ST6Gal-1 levels. ..
  81. Johnston H, Carter M, Larrayoz M, Clarke J, Garbis S, Oscier D, et al. Proteomics Profiling of CLL Versus Healthy B-cells Identifies Putative Therapeutic Targets and a Subtype-independent Signature of Spliceosome Dysregulation. Mol Cell Proteomics. 2018;17:776-791 pubmed publisher
    ..This study highlights the potential of proteomics in the identification of putative CLL therapeutic targets and reveals a subtype-independent protein expression signature in CLL. ..
  82. Bruneau N, Lombardo D. Chaperone function of a Grp 94-related protein for folding and transport of the pancreatic bile salt-dependent lipase. J Biol Chem. 1995;270:13524-33 pubmed
  83. Laroy W, Ameloot P, Contreras R. Characterization of sialyltransferase mutants using surface plasmon resonance. Glycobiology. 2001;11:175-82 pubmed
    ..Only at low salt and high acceptor concentration was association of wild-type ST6GalI with asialofetuin demonstrated...
  84. Cherian R, Jin C, Liu J, Karlsson N, Holgersson J. A Panel of Recombinant Mucins Carrying a Repertoire of Sialylated O-Glycans Based on Different Core Chains for Studies of Glycan Binding Proteins. Biomolecules. 2015;5:1810-31 pubmed publisher
    ..and B3GNT6), or extended core 1 (B3GNT3) chains with or without the type 1 chain-encoding enzyme B3GALT5 and ST6GAL1. Western blot and liquid chromatography-mass spectrometry analysis confirmed the presence of core 1, 2, 3, 4, and ..
  85. Chakraborty A, Dorsett K, Trummell H, Yang E, Oliver P, Bonner J, et al. ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage. J Biol Chem. 2018;293:984-994 pubmed publisher
    ..Together, these findings place ST6Gal-I as a critical player in imparting gemcitabine resistance and as a potential target to restore PDAC chemoresponse. ..
  86. Santos L, Solá S, Rodrigues C, Rebelo de Andrade H. Distinct kinetics and pathways of apoptosis in influenza A and B virus infection. Virus Res. 2015;205:33-40 pubmed publisher
    ..However, this knowledge has not been extended to include influenza type B viruses. In this study, MDCK-SIAT1 cells were infected with influenza A and B strains and the kinetics and pathways of apoptosis post infection were ..
  87. Dörr K, Kilch T, Kappel S, Alansary D, Schwär G, Niemeyer B, et al. Cell type-specific glycosylation of Orai1 modulates store-operated Ca2+ entry. Sci Signal. 2016;9:ra25 pubmed publisher
    ..Knockdown of the sialyltransferase ST6GAL1 reduced α-2,6-linked sialic acids in the glycan structure of Orai1 and was associated with increased Ca(2+) ..
  88. Britain C, Holdbrooks A, Anderson J, Willey C, Bellis S. Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death. J Ovarian Res. 2018;11:12 pubmed publisher
    ..Establishing the tumor-associated ST6Gal-I sialyltransferase as a regulator of EGFR provides novel insight into the role of glycosylation in growth factor signaling and chemoresistance. ..
  89. Korekane H, Matsumoto A, Ota F, Hasegawa T, Misonou Y, Shida K, et al. Involvement of ST6Gal I in the biosynthesis of a unique human colon cancer biomarker candidate, alpha2,6-sialylated blood group type 2H (ST2H) antigen. J Biochem. 2010;148:359-70 pubmed publisher
    ..the ST2H antigen formation, we screened some partially purified candidate enzymes, alpha2,6-sialyltransferases, ST6Gal I and ST6Gal II, and alpha1,2-fucosyltransferases, FUT1 and FUT2 for their activities towards pyridylaminated type ..
  90. Li Q, Wei D, Feng F, Wang X, Li C, Chen Z, et al. ?2,6-linked sialic acid serves as a high-affinity receptor for cancer oncolytic virotherapy with Newcastle disease virus. J Cancer Res Clin Oncol. 2017;143:2171-2181 pubmed publisher
    ..2,6-linked sialic acid served as a high-affinity receptor for NDV and the ST6Gal I sialyltransferase that synthesizes ?2-6 linkage of sialylated N-linked glycans in CHO-K1 cells promoted NDV ..
  91. Wang P, Lee W, Lee Y, Juang C, Chen Y, Chao H, et al. Enhanced expression of alpha 2,6-sialyltransferase ST6Gal I in cervical squamous cell carcinoma. Gynecol Oncol. 2003;89:395-401 pubmed
    Increased messenger ribonucleic acid (mRNA) expression of beta-galactoside alpha 2,6-sialyltransferase I (ST6Gal I) is important in squamous cell carcinoma (SCC) of the cervix...
  92. Yin B, Gao Y, Chung C, Yang S, Blake E, Stuczynski M, et al. Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation. Biotechnol Bioeng. 2015;112:2343-51 pubmed publisher
    ..expression on the N-glycosylation of recombinant erythropoietin (rEPO), a human α2,6-sialyltransferase (ST6Gal1) was expressed in Chinese hamster ovary (CHO-K1) cells...