Gene Symbol: SSPN
Description: sarcospan
Alias: DAGA5, KRAG, NSPN, SPN1, SPN2, sarcospan, K-ras oncogene-associated protein, Kras oncogene-associated, kirsten-ras-associated protein, microspan, nanospan, sarcospan (Kras oncogene-associated gene)
Species: human
Products:     SSPN

Top Publications

  1. Scott A, Elizaga A, Morrell J, Bergen A, Penno M. Characterization of a gene coamplified with Ki-ras in Y1 murine adrenal carcinoma cells that codes for a putative membrane protein. Genomics. 1994;20:227-30 pubmed
    ..The gene, designated KRAG (Ki-ras-associated gene) has a CG-rich first exon and promoter region and a long 3' untranslated region and encodes ..
  2. Peter A, Miller G, Crosbie R. Disrupted mechanical stability of the dystrophin-glycoprotein complex causes severe muscular dystrophy in sarcospan transgenic mice. J Cell Sci. 2007;120:996-1008 pubmed
    ..Within the dystrophin-glycoprotein complex, the sarcoglycans and sarcospan constitute a subcomplex of transmembrane proteins that stabilize alpha-dystroglycan, a receptor for laminin and ..
  3. Heighway J, Betticher D, Hoban P, Altermatt H, Cowen R. Coamplification in tumors of KRAS2, type 2 inositol 1,4,5 triphosphate receptor gene, and a novel human gene, KRAG. Genomics. 1996;35:207-14 pubmed
    ..region of DNA, coamplified in tumors with KRAS2, resulted in the identification of the human homologue of the mouse KRAG gene...
  4. Crosbie R, Heighway J, Venzke D, Lee J, Campbell K. Sarcospan, the 25-kDa transmembrane component of the dystrophin-glycoprotein complex. J Biol Chem. 1997;272:31221-4 pubmed
    ..Here we report the cloning and characterization of sarcospan, a unique 25-kDa member of this complex...
  5. Crosbie R, Lim L, Moore S, Hirano M, Hays A, Maybaum S, et al. Molecular and genetic characterization of sarcospan: insights into sarcoglycan-sarcospan interactions. Hum Mol Genet. 2000;9:2019-27 pubmed
    ..We show that sarcospan, a novel tetraspan-like protein, is also lost in patients with either a complete or partial loss of the ..
  6. Park S, Nam J, Ahn C, Kim Y. Anti-diabetic properties of different fractions of Korean red ginseng. J Ethnopharmacol. 2019;236:220-230 pubmed publisher
    ..There is no study reporting the differentiated effects of ginsenosides (Spn) and non-saponin fractions (NSpn) of KRG on glycemic control...
  7. Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, et al. Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. Mol Cell Biol. 2010;30:2057-74 pubmed publisher
    ..During the prior conjugation of haploid cells, the four vegetatively expressed septins (Spn1, Spn2, Spn3, and Spn4) coassemble at the fusion site and are necessary for its normal morphogenesis...
  8. McDonald S, Close D, Xin H, Formosa T, Hill C. Structure and biological importance of the Spn1-Spt6 interaction, and its regulatory role in nucleosome binding. Mol Cell. 2010;40:725-35 pubmed publisher
    Eukaryotic transcription and mRNA processing depend upon the coordinated interactions of many proteins, including Spn1 and Spt6, which are conserved across eukaryotes, are essential for viability, and associate with each other in some of ..
  9. Dronamraju R, Kerschner J, Peck S, Hepperla A, Adams A, Hughes K, et al. Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction. Cell Rep. 2018;25:3476-3489.e5 pubmed publisher
    ..Mechanistically, we show that CKII phosphorylation of Spt6 promotes the interaction of Spt6 with Spn1, a binding partner required for chromatin reassembly and full recruitment of Spt6 to genes...

More Information


  1. Berger M, Pantet O, Jacquelin Ravel N, Charrière M, Schmidt S, Becce F, et al. Supplemental parenteral nutrition improves immunity with unchanged carbohydrate and protein metabolism in critically ill patients: The SPN2 randomized tracer study. Clin Nutr. 2018;: pubmed publisher
    ..NCT02022813 at ..
  2. Thurston A, Radebaugh C, Almeida A, Argueso J, Stargell L. Genome Instability Is Promoted by the Chromatin-Binding Protein Spn1 in Saccharomyces cerevisiae. Genetics. 2018;210:1227-1237 pubmed publisher
    ..Using Saccharomyces cerevisiae, we examined the role of Spn1 (Suppresses postrecruitment gene number 1), a chromatin-binding and transcription elongation factor, in DNA-damage ..
  3. Liang X, Sun P, Peng X, Liu J, Chen Z, Shan X. [Significance of the seated supine positioning nystagmus for the diagnosis of benign paroxysmal positional vertigo]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017;31:703-707 pubmed publisher
    b>Objective:To observe and assess the significance of seated supine positioning nystagmus (SSPN) in the diagnosis of benign paroxysmal positional vertigo(BPPV)...
  4. Nielsen J, Thorolfsdottir R, Fritsche L, Zhou W, Skov M, Graham S, et al. Biobank-driven genomic discovery yields new insight into atrial fibrillation biology. Nat Genet. 2018;50:1234-1239 pubmed publisher
    ..or near genes important for striated muscle function and integrity (for example, CFL2, MYH7, PKP2, RBM20, SGCG, SSPN)...
  5. Lee K, Ranger M, Meneghini M. Combinatorial Genetic Control of Rpd3S Through Histone H3K4 and H3K36 Methylation in Budding Yeast. G3 (Bethesda). 2018;8:3411-3420 pubmed publisher
    ..found that levels of H3K4me modulated temperature sensitive alleles of the transcriptional elongation complex Spt6-Spn1 through an unknown H3K4me effector pathway...
  6. Marshall J, Oh J, Chou E, Lee J, Holmberg J, Burkin D, et al. Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and α7 integrin. Hum Mol Genet. 2015;24:2011-22 pubmed publisher
    ..We previously demonstrated that overexpression of sarcospan, a dystrophin- and utrophin-binding protein, ameliorates mdx muscular dystrophy...
  7. Liu C, Buchkovich M, Winkler T, Heid I, Borecki I, Fox C, et al. Multi-ethnic fine-mapping of 14 central adiposity loci. Hum Mol Genet. 2014;23:4738-44 pubmed publisher
    ..1). Trans-ethnic analyses at five loci (TBX15-WARS2, LYPLAL1, ADAMTS9, LY86 and ITPR2-SSPN) substantially narrowed the signals to smaller sets of variants, some of which are in regions that have evidence of ..
  8. Boulay A, Saubaméa B, Cisternino S, Mignon V, Mazeraud A, Jourdren L, et al. The Sarcoglycan complex is expressed in the cerebrovascular system and is specifically regulated by astroglial Cx30 channels. Front Cell Neurosci. 2015;9:9 pubmed publisher
    ..the cerebrovascular system and showed the presence of α-, β-, δ-, γ-, ε- and ζ- SG, as well as Sarcospan. Their expression was however not modified in Cx30(Δ/Δ)...
  9. Miyazaki M, Sakai S, Ritalahti K, Saito Y, Yamanaka Y, Saito Y, et al. Sphaerochaeta multiformis sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Sphaerochaeta. Int J Syst Evol Microbiol. 2014;64:4147-54 pubmed publisher
    ..4 % sequence identity), Sphaerochaeta globosa Buddy(T) (86.7 %) and Sphaerochaeta coccoides SPN1(T) (85.4 %)...
  10. Zhang J, Chen J, Ren J, Guo W, Li X, Chen R, et al. Biocompatible semiconducting polymer nanoparticles as robust photoacoustic and photothermal agents revealing the effects of chemical structure on high photothermal conversion efficiency. Biomaterials. 2018;181:92-102 pubmed publisher
    ..In particular, SPN1 with the longest ?-conjugation length and the highest mass extinction coefficient which are beneficial for ..
  11. Miller G, Moore C, Terry R, La Riviere T, Mitchell A, Piggott R, et al. Preventing phosphorylation of dystroglycan ameliorates the dystrophic phenotype in mdx mouse. Hum Mol Genet. 2012;21:4508-20 pubmed
    ..other DGC components were restored to the sarcolemma including α-sarcoglycan, α-/β-dystroglycan and sarcospan. Furthermore, Dag1(Y890F/Y890F)/mdx showed a significant resistance to muscle damage and force loss following ..
  12. Voisin S, Almén M, Zheleznyakova G, Lundberg L, Zarei S, Castillo S, et al. Many obesity-associated SNPs strongly associate with DNA methylation changes at proximal promoters and enhancers. Genome Med. 2015;7:103 pubmed publisher
  13. McCullough L, Connell Z, Petersen C, Formosa T. The Abundant Histone Chaperones Spt6 and FACT Collaborate to Assemble, Inspect, and Maintain Chromatin Structure in Saccharomyces cerevisiae. Genetics. 2015;201:1031-45 pubmed publisher
    ..functional domains, including a natively unstructured 30-residue N-terminal region that binds competitively with Spn1 or nucleosomes...
  14. Peter A, Miller G, Capote J, DiFranco M, Solares Pérez A, Wang E, et al. Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F. Skelet Muscle. 2017;7:11 pubmed publisher
    ..Alternative splicing of proteins from the SG-SSPN complex produces δ-SG3, microspan, and nanospan that localize to the ZSR and the triadic SR, where they may play a role in regulating resting ..
  15. Kievit R, Brandmaier A, Ziegler G, van Harmelen A, de Mooij S, Moutoussis M, et al. Developmental cognitive neuroscience using latent change score models: A tutorial and applications. Dev Cogn Neurosci. 2018;33:99-117 pubmed publisher
    ..In an adolescent development cohort (NSPN, N = 176, two waves) we find greater variability in cortical thinning in males than in females...
  16. Lee K, Chen Z, Jiang R, Meneghini M. H3K4 Methylation Dependent and Independent Chromatin Regulation by JHD2 and SET1 in Budding Yeast. G3 (Bethesda). 2018;8:1829-1839 pubmed publisher
    ..findings implicate JHD2 in genetic inhibition of the histone chaperone complexes Spt16-Pob3 (FACT) and Spt6-Spn1 This targeted screen also revealed that JHD2 inhibits the Nrd1-Nab3-Sen1 (NNS) transcription termination ..
  17. Cox M, Evans J, Davis A, Guo L, Levy J, Starr Moss A, et al. Exome sequencing reveals independent SGCD deletions causing limb girdle muscular dystrophy in Boston terriers. Skelet Muscle. 2017;7:15 pubmed publisher
    ..of the dystrophic histological phenotype on muscle histology, and demonstration of the absence of sarcoglycan-sarcospan complex by immunostaining, whole exome sequencing was performed on five Boston terriers: one affected dog and ..
  18. Parvatiyar M, Marshall J, Nguyen R, Jordan M, Richardson V, Roos K, et al. Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy. J Am Heart Assoc. 2015;4: pubmed publisher
    ..We have previously demonstrated that a dystrophin-associated protein, sarcospan (SSPN), ameliorated Duchenne muscular dystrophy skeletal muscle degeneration by activating compensatory pathways ..
  19. Tarakci H, Berger J. The sarcoglycan complex in skeletal muscle. Front Biosci (Landmark Ed). 2016;21:744-56 pubmed
    ..Together with sarcospan, the sarcoglycan complex binds other components of the dystrophin-associated glycoprotein complex and integrates ..
  20. Samanta A, Chakraborty H, Bhattacharya M, Ghosh J, Sreemany M, Bysakh S, et al. Nanotribological response of a plasma nitrided bio-steel. J Mech Behav Biomed Mater. 2017;65:584-599 pubmed publisher
    ..For ease of comparison these plasma nitrided samples were termed as SSPN. The experimental results confirmed the formations of an embedded nitrided metal layer zone (ENMLZ) and an ..
  21. Noguchi S, Wakabayashi E, Imamura M, Yoshida M, Ozawa E. Formation of sarcoglycan complex with differentiation in cultured myocytes. Eur J Biochem. 2000;267:640-8 pubmed
    ..sarcoglycan complex is formed in the endoplasmic reticulum and then associates with the dystroglycan complex and sarcospan en route from the Golgi apparatus to the cell surface...
  22. Hiyama T, Haruma K, Kitadai Y, Masuda H, Miyamoto M, Tanaka S, et al. K-ras mutation in helicobacter pylori-associated chronic gastritis in patients with and without gastric cancer. Int J Cancer. 2002;97:562-6 pubmed
  23. Chew E, Tan J, Bahta A, Ho B, Liu X, Lim T, et al. Differential Expression between Human Dermal Papilla Cells from Balding and Non-Balding Scalps Reveals New Candidate Genes for Androgenetic Alopecia. J Invest Dermatol. 2016;136:1559-1567 pubmed publisher
    ..Further, our data suggest TWIST1 (twist family basic helix-loop-helix transcription factor 1) and SSPN (sarcospan) to be the functionally relevant AGA genes at the 7p21.1 and 12p12.1 risk loci, respectively...
  24. Gibbs E, Marshall J, Ma E, Nguyen T, Hong G, Lam J, et al. High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD. Hum Mol Genet. 2016;25:5395-5406 pubmed publisher
    ..Previous work from our lab has shown that a small transmembrane protein called sarcospan (SSPN) can enhance the recruitment of adhesion complex proteins to the cell surface...
  25. Yoh S, Cho H, Pickle L, Evans R, Jones K. The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export. Genes Dev. 2007;21:160-74 pubmed
    ..Thus binding of Spt6 to Ser2-P RNAPII provides a cotranscriptional mechanism to recruit Iws1, REF1/Aly, and associated mRNA processing, surveillance, and export factors to responsive genes. ..
  26. Miller G, Peter A, Espinoza E, Heighway J, Crosbie R. Over-expression of Microspan, a novel component of the sarcoplasmic reticulum, causes severe muscle pathology with triad abnormalities. J Muscle Res Cell Motil. 2006;27:545-58 pubmed
    b>Sarcospan (SSPN) is a core component of the dystrophin-glycoprotein complex (DGC). Multiple SSPN transcripts are ubiquitously expressed and SSPN splicing is disrupted in many lung tumors, suggesting the importance of SSPN-related mRNAs...
  27. O Brien K, Engle E, Kunkel L. Analysis of human sarcospan as a candidate gene for CFEOM1. BMC Genet. 2001;2:3 pubmed
    ..2 - q12). Sarcospan is a member of the dystrophin associated protein complex in skeletal and extraocular muscle and maps to human ..
  28. Hughes D, Marcotte G, Marshall A, West D, Baehr L, Wallace M, et al. Age-related Differences in Dystrophin: Impact on Force Transfer Proteins, Membrane Integrity, and Neuromuscular Junction Stability. J Gerontol A Biol Sci Med Sci. 2017;72:640-648 pubmed publisher
    ..To compensate for the loss of dystrophin protein, aged muscle contained increased ?-sarcoglycan, syntrophin, sarcospan, laminin, ?1-integrin, desmuslin, and the Z-line proteins ?-actinin and desmin...
  29. Wohlwend D, Strasser A, Dickmanns A, Ficner R. Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus. J Mol Biol. 2007;374:1129-38 pubmed
    ..U snRNPs), is mediated by a nuclear import receptor adaptor couple of importin beta (Imp beta) and snurportin1 (SPN1)...
  30. Panzer A, Regmi S, Cormier D, Danzo M, Chen I, Winston J, et al. Nkx2-5 and Sarcospan genetically interact in the development of the muscular ventricular septum of the heart. Sci Rep. 2017;7:46438 pubmed publisher
    ..We describe here a genetic interaction between Nkx2-5 and Sarcospan (Sspn) that affects the risk of muscular VSD in mice...
  31. Yoshida M, Hama H, Ishikawa Sakurai M, Imamura M, Mizuno Y, Araishi K, et al. Biochemical evidence for association of dystrobrevin with the sarcoglycan-sarcospan complex as a basis for understanding sarcoglycanopathy. Hum Mol Genet. 2000;9:1033-40 pubmed
    ..It was suggested from one of the complexes prepared that the sarco-glycan-sarcospan complex (the sarcoglycan complex associated with sarcospan) is associated with syntrophin and/or dystrobrevin...
  32. Noda N, Matsuzoe D, Konno T, Kawahara K, Yamashita Y, Shirakusa T. Risk for K-ras gene mutations in smoking-induced lung cancer is associated with cytochrome P4501A1 and glutathione S-transferase micro1 polymorphisms. Oncol Rep. 2004;12:773-9 pubmed
  33. Tomita S, Kawamata H, Imura J, Omotehara F, Ueda Y, Fujimori T. Frequent polymorphism of peroxisome proliferator activated receptor gamma gene in colorectal cancer containing wild-type K-ras gene. Int J Mol Med. 2002;9:485-8 pubmed
    ..These findings suggest that PPARgamma gene polymorphism may be implicated with the development of colorectal cancers, in which K-ras gene is not mutated. ..
  34. Matsuzoe D, Hideshima T, Iwasaki A, Yoneda S, Kawahara K, Shirakusa T, et al. Glutathione S-transferase mu1 null genotype is associated with K-ras gene mutation in lung adenocarcinoma among smokers. Carcinogenesis. 2001;22:1327-30 pubmed
    ..These findings suggest that the cause of K-ras gene mutation in smokers with lung adenocarcinoma may be in part an accumulation of BP diol epoxide which is not well detoxified in individuals with the GSTM1 null genotype. ..
  35. Heid I, Jackson A, Randall J, Winkler T, Qi L, Steinthorsdottir V, et al. Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distribution. Nat Genet. 2010;42:949-60 pubmed publisher
    ..We identified 13 new loci in or near RSPO3, VEGFA, TBX15-WARS2, NFE2L3, GRB14, DNM3-PIGC, ITPR2-SSPN, LY86, HOXC13, ADAMTS9, ZNRF3-KREMEN1, NISCH-STAB1 and CPEB4 (P = 1.9 × 10⁻⁹ to P = 1...
  36. Peter A, Marshall J, Crosbie R. Sarcospan reduces dystrophic pathology: stabilization of the utrophin-glycoprotein complex. J Cell Biol. 2008;183:419-27 pubmed publisher
    ..We show that sarcospan (SSPN), a unique tetraspanin-like component of the DGC, ameliorates muscular dystrophy in dystrophin-deficient ..
  37. Wakayama Y, Inoue M, Kojima H, Yamashita S, Shibuya S, Jimi T, et al. Reduced expression of sarcospan in muscles of Fukuyama congenital muscular dystrophy. Histol Histopathol. 2008;23:1425-38 pubmed publisher
    Expression profiles of sarcospan in muscles with muscular dystrophies are scarcely reported...