Gene Symbol: SRPK1
Description: SRSF protein kinase 1
Alias: SFRSK1, SRSF protein kinase 1, SFRS protein kinase 1, SR-protein-specific kinase 1, serine/arginine-rich splicing factor kinase 1, serine/threonine-protein kinase SRPK1
Species: human
Products:     SRPK1

Top Publications

  1. Souki S, Sandri Goldin R. Arginine methylation of the ICP27 RGG box regulates the functional interactions of ICP27 with SRPK1 and Aly/REF during herpes simplex virus 1 infection. J Virol. 2009;83:8970-5 pubmed publisher
    ..interactions; therefore, we examined the effect of hypomethylation on ICP27's interactions with cellular proteins SRPK1 and Aly/REF, which bind to ICP27 through the RGG box region...
  2. Koizumi J, Okamoto Y, Onogi H, Mayeda A, Krainer A, Hagiwara M. The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs). J Biol Chem. 1999;274:11125-31 pubmed
    ..Recombinant SRPK1 and SRPK2 bound to and phosphorylated GST-SF2/ASF in vitro...
  3. Gui J, Lane W, Fu X. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature. 1994;369:678-82 pubmed
    ..We have now identified and cloned a kinase, SRPK1, which is regulated by the cell cycle and is specific for SR proteins; this kinase is related to a Caenorhabditis ..
  4. Colwill K, Feng L, Yeakley J, Gish G, Caceres J, Pawson T, et al. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996;271:24569-75 pubmed
    ..Two protein kinases have been cloned that can phosphorylate SR proteins in vitro: SRPK1 and Clk/Sty...
  5. Tronchere H, Wang J, Fu X. A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA. Nature. 1997;388:397-400 pubmed
    ..Therefore, Urp and U2AF35 may independently position RS-domain-containing factors within spliceosomes. ..
  6. Wang H, Lin W, Dyck J, Yeakley J, Songyang Z, Cantley L, et al. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998;140:737-50 pubmed
    ..Two kinases, SR protein-specific kinase (SRPK1) and Clk/Sty, have been shown to phosphorylate the SR family of splicing factors...
  7. Nikolakaki E, Kohen R, Hartmann A, Stamm S, Georgatsou E, Giannakouros T. Cloning and characterization of an alternatively spliced form of SR protein kinase 1 that interacts specifically with scaffold attachment factor-B. J Biol Chem. 2001;276:40175-82 pubmed
    ..Human SRPK1, which was first identified as a kinase specific for the SR family of splicing factors, is located on chromosome ..
  8. Long Y, Sou W, Yung K, LIU H, Wan S, Li Q, et al. Distinct mechanisms govern the phosphorylation of different SR protein splicing factors. J Biol Chem. 2019;294:1312-1327 pubmed publisher
    ..The phosphorylation mechanism of the prototypic SRSF1 by serine-arginine protein kinase 1 (SRPK1) has been well-studied, but little is known about the phosphorylation of other SR protein members...
  9. Tzelepis K, De Braekeleer E, Aspris D, Barbieri I, Vijayabaskar M, Liu W, et al. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4. Nat Commun. 2018;9:5378 pubmed publisher
    We recently identified the splicing kinase gene SRPK1 as a genetic vulnerability of acute myeloid leukemia (AML)...

More Information

Publications105 found, 100 shown here

  1. Huang J, Zhou Y, Xue X, Jiang L, Du J, Cui Y, et al. SRPIN340 protects heart muscle from oxidative damage via SRPK1/2 inhibition-mediated AKT activation. Biochem Biophys Res Commun. 2019;510:97-103 pubmed publisher
    SRPIN340, a selective serine-arginine protein kinase 1/2 (SRPK1/2) inhibitor, has been shown to have antiviral and anti-angiogenesis effects. However, its role in the heart is unknown...
  2. Wagner K, El Maï M, Ladomery M, Belali T, Leccia N, Michiels J, et al. Altered VEGF Splicing Isoform Balance in Tumor Endothelium Involves Activation of Splicing Factors Srpk1 and Srsf1 by the Wilms' Tumor Suppressor Wt1. Cells. 2019;8: pubmed publisher
    ..the Wilms' tumor suppressor 1 (WT1) suppresses the Serine/arginine-rich protein-specific splicing factor kinase (SRPK1), and indirectly Serine/arginine-rich splicing factor 1 (Srsf1) activity, and alters VEGF splicing...
  3. Patel M, Sachidanandan M, Adnan M. Serine arginine protein kinase 1 (SRPK1): a moonlighting protein with theranostic ability in cancer prevention. Mol Biol Rep. 2019;46:1487-1497 pubmed publisher
    Serine/arginine protein kinase 1 (SRPK1); a versatile functional moonlighting protein involved in varied cellular activities comprised of cell cycle progression, innate immune response, chromatin reorganization, negative and positive ..
  4. Ngo J, Chakrabarti S, Ding J, Velazquez Dones A, Nolen B, Aubol B, et al. Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2. Mol Cell. 2005;20:77-89 pubmed
    ..Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2...
  5. Yi N, Xiao M, Jiang F, Liu Z, Ni W, Lu C, et al. SRPK1 is a poor prognostic indicator and a novel potential therapeutic target for human colorectal cancer. Onco Targets Ther. 2018;11:5359-5370 pubmed publisher
    Serine/arginine protein kinase 1 (SRPK1) is a protein kinase that belongs to the serine/arginine-rich domain family of splicing factors which are essential for splice-site selection, especially the modulation for RNA metabolism, ..
  6. Mavrou A, Brakspear K, Hamdollah Zadeh M, Damodaran G, Babaei Jadidi R, Oxley J, et al. Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer. Oncogene. 2015;34:4311-9 pubmed publisher
    ..splice isoforms is controlled by the splicing factor SRSF1, phosphorylated by serine-arginine protein kinase 1 (SRPK1)...
  7. Xu Q, Liu X, Liu Z, Zhou Z, Wang Y, Tu J, et al. MicroRNA-1296 inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting SRPK1-mediated PI3K/AKT pathway. Mol Cancer. 2017;16:103 pubmed publisher
    ..In addition, miR-1296 inversely regulated SRPK1 abundance by directly binding to its 3'-UTR, which subsequently resulted in suppression of p-AKT...
  8. Mavrou A, Oltean S. SRPK1 inhibition in prostate cancer: A novel anti-angiogenic treatment through modulation of VEGF alternative splicing. Pharmacol Res. 2016;107:276-281 pubmed publisher
  9. Aubol B, Keshwani M, Fattet L, Adams J. Mobilization of a splicing factor through a nuclear kinase-kinase complex. Biochem J. 2018;475:677-690 pubmed publisher
    ..of serine-proline dipeptides in the prototype SR protein SRSF1, an unrelated enzyme known as SR protein kinase 1 (SRPK1) performs the same function but does not efficiently modify these dipeptides in SRSF1...
  10. Li X, Song J, Liu J, Wu S, Wang L, Gong L, et al. Serine-arginine protein kinase 1 is associated with breast cancer progression and poor patient survival. Med Oncol. 2014;31:83 pubmed publisher
    ..Serine-arginine protein kinase 1 (SRPK1), a member of SR kinase family, phosphorylates the SR splicing factors which plays essential roles in normal cell ..
  11. Prescott E, Brimacombe C, Hartley M, Bell I, Graham S, Roberts S. Human papillomavirus type 1 E1^E4 protein is a potent inhibitor of the serine-arginine (SR) protein kinase SRPK1 and inhibits phosphorylation of host SR proteins and of the viral transcription and replication regulator E2. J Virol. 2014;88:12599-611 pubmed publisher
    The serine-arginine-specific protein kinase SRPK1 is a common binding partner of the E1^E4 protein of diverse human papillomavirus types...
  12. Hulse R, Beazley Long N, Hua J, Kennedy H, Prager J, Bevan H, et al. Regulation of alternative VEGF-A mRNA splicing is a therapeutic target for analgesia. Neurobiol Dis. 2014;71:245-59 pubmed publisher
    ..balance of VEGF-A isoforms switches to greater expression of VEGF-Axxxa compared to VEGF-Axxxb, through an SRPK1-dependent pre-mRNA splicing mechanism...
  13. Hulse R, Drake R, Bates D, Donaldson L. The control of alternative splicing by SRSF1 in myelinated afferents contributes to the development of neuropathic pain. Neurobiol Dis. 2016;96:186-200 pubmed publisher
    ..Serine arginine protein kinase 1 (SRPK1) is the principal route of SRSF1 activation...
  14. Gammons M, Fedorov O, Ivison D, Du C, Clark T, Hopkins C, et al. Topical antiangiogenic SRPK1 inhibitors reduce choroidal neovascularization in rodent models of exudative AMD. Invest Ophthalmol Vis Sci. 2013;54:6052-62 pubmed publisher
    ..Serine-rich protein kinase-1 (SRPK1) has been identified as a regulator of pro-angiogenic VEGF splicing by phosphorylating serine-rich splicing factor-..
  15. Han X, Yang J, Jia Z, Wei P, Zhang H, Lv W, et al. Knockdown of Serine-Arginine Protein Kinase 1 Inhibits the Growth and Migration in Renal Cell Carcinoma Cells. Oncol Res. 2017;25:389-395 pubmed publisher
    The pre-mRNA splicing regulator serine-arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes...
  16. Morooka S, Hoshina M, Kii I, Okabe T, Kojima H, Inoue N, et al. Identification of a Dual Inhibitor of SRPK1 and CK2 That Attenuates Pathological Angiogenesis of Macular Degeneration in Mice. Mol Pharmacol. 2015;88:316-25 pubmed publisher
    ..Our previous studies using SRPIN340, a small molecule inhibitor of SRPK1 (serine-arginine protein kinase 1), demonstrated that SRPK1 is a potential target for the development of ..
  17. Moreira G, Lima G, Siqueira R, Barros M, Adjanohoun A, Santos V, et al. Antimetastatic effect of the pharmacological inhibition of serine/arginine-rich protein kinases (SRPK) in murine melanoma. Toxicol Appl Pharmacol. 2018;356:214-223 pubmed publisher
    ..Moreover, the effect of the treatments on SRPK1 nuclear translocation and SR protein phosphorylation was observed...
  18. Karakama Y, Sakamoto N, Itsui Y, Nakagawa M, Tasaka Fujita M, Nishimura Sakurai Y, et al. Inhibition of hepatitis C virus replication by a specific inhibitor of serine-arginine-rich protein kinase. Antimicrob Agents Chemother. 2010;54:3179-86 pubmed publisher
    ..Overexpression of SRPK1 or SRPK2 resulted in augmentation of HCV replication, while small interfering RNA (siRNA) knockdown of the SRPKs ..
  19. Brown M, Dobrikov M, Gromeier M. Mitogen-activated protein kinase-interacting kinase regulates mTOR/AKT signaling and controls the serine/arginine-rich protein kinase-responsive type 1 internal ribosome entry site-mediated translation and viral oncolysis. J Virol. 2014;88:13149-60 pubmed publisher
  20. Basei F, Meirelles G, Righetto G, Dos Santos Migueleti D, Smetana J, Kobarg J. New interaction partners for Nek4.1 and Nek4.2 isoforms: from the DNA damage response to RNA splicing. Proteome Sci. 2015;13:11 pubmed publisher
    ..Among the interacting partners, we found important proteins such as ANT3, Whirlin, PCNA, 14-3-3ε, SRSF1, SRSF2, SRPK1 and hNRNPs proteins...
  21. Bell I, Martin A, Roberts S. The E1circumflexE4 protein of human papillomavirus interacts with the serine-arginine-specific protein kinase SRPK1. J Virol. 2007;81:5437-48 pubmed
    ..Here, we show that the serine-arginine (SR)-specific protein kinase SRPK1 is a novel binding partner of HPV type 1 (HPV1) E1circumflexE4...
  22. Mytilinaios D, Tsamis K, Nikolakaki E, Giannakouros T. Distribution of SRPK1 in human brain. J Chem Neuroanat. 2012;43:20-7 pubmed publisher
    ..b>SRPK1 is known to be expressed in the mammalian central nervous system...
  23. Yoon C, Kim D, Kim S, Park G, Hur D, Yang J, et al. MiR-9 regulates the post-transcriptional level of VEGF165a by targeting SRPK-1 in ARPE-19 cells. Graefes Arch Clin Exp Ophthalmol. 2014;252:1369-76 pubmed publisher
    ..Hypoxic stress decreased the miR-9 level in ARPE-19 cells, which increased the transcriptional level of SRPK-1, resulting in alternative splicing shift to pro-angiogenic isoforms of VEGF165 in human retinal pigment epithelial cells. ..
  24. Bowler E, Porazinski S, Uzor S, Thibault P, Durand M, Lapointe E, et al. Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells. BMC Cancer. 2018;18:355 pubmed publisher
    ..The expression of the splice factors SRSF1, SRSF2, SRSF3, SAM68, HuR, hnRNPA1, and of the splice factor kinases SRPK1 and CLK1 increased significantly in hypoxia...
  25. Ngo J, Giang K, Chakrabarti S, Ma C, Huynh N, Hagopian J, et al. A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1. Mol Cell. 2008;29:563-76 pubmed publisher
    The 2.9 A crystal structure of the core SRPK1:ASF/SF2 complex reveals that the N-terminal half of the basic RS domain of ASF/SF2, which is destined to be phosphorylated, is bound to an acidic docking groove of SRPK1 distal to the active ..
  26. Zheng Y, Fu X, Ou J. Suppression of hepatitis B virus replication by SRPK1 and SRPK2 via a pathway independent of the phosphorylation of the viral core protein. Virology. 2005;342:150-8 pubmed
    ..In this report, we tested the role of SRPK1 and SRPK2 in HBV replication and found that both of them could suppress HBV replication by reducing the packaging ..
  27. Chang Y, Li L, Zhang L, Guo X, Feng Z, Zhou J, et al. Plexin-B1 indirectly affects glioma invasiveness and angiogenesis by regulating the RhoA/αvβ3 signaling pathway and SRPK1. Tumour Biol. 2016;37:11225-36 pubmed publisher
    ..potential, cell migration and invasion, angiogenesis, PI3K and Akt phosphorylation, and also the levels of SRPK1 and αvβ3 in glioma cells and animal glioma tissues...
  28. Golubtsova N, Kornilova N, Gunin A. [Age-related changes in the content of serine-arginine protein kinase 1 (SRPK1) in human dermis.]. Adv Gerontol. 2017;30:652-658 pubmed
    The aim of our work was to examine content of serine-arginine protein kinase 1 (SRPK1) in human dermis at different ages (from 20 weeks of pregnancy to 85 years old)...
  29. Mylonis I, Giannakouros T. Protein kinase CK2 phosphorylates and activates the SR protein-specific kinase 1. Biochem Biophys Res Commun. 2003;301:650-6 pubmed
    ..Mammalian SRPK1 has been considered as a constitutively active kinase that is predominantly expressed in testis...
  30. Tsianou D, Nikolakaki E, Tzitzira A, Bonanou S, Giannakouros T, Georgatsou E. The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2. FEBS J. 2009;276:5212-27 pubmed publisher
    ..protein scaffold attachment factor B1 (SAFB1) via its unique N-terminal domain, which differentiates it from SRPK1. In this study, we show that SAFB1 inhibits the activity of both SRPK1a and SRPK1 in vitro and that its RE-rich ..
  31. Jakubauskiene E, Vilys L, Makino Y, Poellinger L, Kanopka A. Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia. J Biol Chem. 2015;290:18079-89 pubmed publisher
    ..We also show that expression of SR protein kinases (CLK1, SRPK1, SRPK2) in hypoxic cells is elevated at mRNA and protein levels...
  32. Smet Nocca C, Page A, Cantrelle F, Nikolakaki E, Landrieu I, Giannakouros T. The O-?-linked N-acetylglucosaminylation of the Lamin B receptor and its impact on DNA binding and phosphorylation. Biochim Biophys Acta Gen Subj. 2018;1862:825-835 pubmed publisher
    ..that O-GlcNAcylation hampers DNA binding while it marginally affects RS domain phosphorylation mediated by SRPK1, Akt2 and cdk1 kinases...
  33. Wang H, Arden K, Bermingham J, Viars C, Lin W, Boyer A, et al. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. Genomics. 1999;57:310-5 pubmed
    ..We have previously characterized SRPK1 (SFRSK1) and SRPK2 (SFRSK2), which are highly specific protein kinases for the SR family of splicing factors...
  34. Giannakouros T, Nikolakaki E, Mylonis I, Georgatsou E. Serine-arginine protein kinases: a small protein kinase family with a large cellular presence. FEBS J. 2011;278:570-86 pubmed publisher
    ..Fifteen years of research subsequent to the purification and cloning of human SRPK1 as a SR splicing factor-phosphorylating protein have lead to the accumulation of information on the function and ..
  35. Hatcher J, Wu G, Zeng C, Zhu J, Meng F, Patel S, et al. SRPKIN-1: A Covalent SRPK1/2 Inhibitor that Potently Converts VEGF from Pro-angiogenic to Anti-angiogenic Isoform. Cell Chem Biol. 2018;25:460-470.e6 pubmed publisher
    ..Kinome-wide profiling demonstrates its selectivity for SRPK1/2, and SRPKIN-1 attenuates SR protein phosphorylation at submicromolar concentrations...
  36. Mylonis I, Drosou V, Brancorsini S, Nikolakaki E, Sassone Corsi P, Giannakouros T. Temporal association of protamine 1 with the inner nuclear membrane protein lamin B receptor during spermiogenesis. J Biol Chem. 2004;279:11626-31 pubmed
    ..Our data suggest that docking of protamine 1 to the nuclear envelope is an important intermediate step in spermiogenesis and reveal a novel role for SR protein kinases and p32. ..
  37. Lukasiewicz R, Velazquez Dones A, Huynh N, Hagopian J, Fu X, Adams J, et al. Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions. J Biol Chem. 2007;282:23036-43 pubmed
    ..the C terminus, and approximately 12 of these serines are processively phosphorylated by the SR protein kinase 1 (SRPK1)...
  38. Aubol B, Adams J. Recruiting a silent partner for activation of the protein kinase SRPK1. Biochemistry. 2014;53:4625-34 pubmed publisher
    ..We now show that sequences distal to the SRPK1 consensus region in the RS domain of the prototype SR protein SRSF1 are not passive as originally thought but ..
  39. Gammons M, Lucas R, Dean R, Coupland S, Oltean S, Bates D. Targeting SRPK1 to control VEGF-mediated tumour angiogenesis in metastatic melanoma. Br J Cancer. 2014;111:477-85 pubmed publisher
    ..with a splicing switch to pro-angiogenic VEGF-A, previously shown to be regulated by SRSF1 phosphorylation by SRPK1. Here, we show a novel approach to preventing angiogenesis-targeting splicing factor kinases that are highly ..
  40. Xu X, Wei Y, Wang S, Luo M, Zeng H. Serine-arginine protein kinase 1 (SRPK1) is elevated in gastric cancer and plays oncogenic functions. Oncotarget. 2017;8:61944-61957 pubmed publisher
    Serine-arginine protein kinase 1 (SRPK1) phosphorylates proteins involved in the regulation of several mRNA processing pathways including alternative splicing...
  41. Lin J. Multi-posttranscriptional regulations lessen the repressive effect of SRPK1 on brown adipogenesis. Biochim Biophys Acta Mol Cell Biol Lipids. 2018;1863:503-514 pubmed publisher
    ..the reprogrammed splicing profile and reduced expression of serine/arginine-rich splicing factor protein kinase 1 (SRPK1) throughout the development of brown adipose tissue (BAT)...
  42. Zhou B, Li Y, Deng Q, Wang H, Wang Y, Cai B, et al. SRPK1 contributes to malignancy of hepatocellular carcinoma through a possible mechanism involving PI3K/Akt. Mol Cell Biochem. 2013;379:191-9 pubmed publisher
    ..In the present study, we found that the serine-arginine protein kinase 1 (SRPK1) might be involved in hepatocellular carcinoma (HCC) proliferation from a kinome screen using a loss-of-function ..
  43. Papoutsopoulou S, Nikolakaki E, Giannakouros T. SRPK1 and LBR protein kinases show identical substrate specificities. Biochem Biophys Res Commun. 1999;255:602-7 pubmed
    ..Two SR Protein-specific Kinases, SRPK1 and SRPK2, have been shown to phosphorylate specifically the RS motifs of the SR family of splicing factors and ..
  44. Aubol B, Chakrabarti S, Ngo J, Shaffer J, Nolen B, Fu X, et al. Processive phosphorylation of alternative splicing factor/splicing factor 2. Proc Natl Acad Sci U S A. 2003;100:12601-6 pubmed
    ..Here, we carried out kinetic experiments specially developed to investigate how SRPK1 phosphorylates the model human SR protein, ASF/SF2...
  45. Lee C, Hague L, Li H, Donnelly R. Identification of toposome, a novel multisubunit complex containing topoisomerase IIalpha. Cell Cycle. 2004;3:638-47 pubmed
    ..containing two ATPase/helicase proteins (RNA helicase A and RHII/Gu), one serine/threonine protein kinase (SRPK1), one HMG protein (SSRP1), and two pre-mRNA splicing factors (PRP8 and hnRNP C)...
  46. Ma C, Velazquez Dones A, Hagopian J, Ghosh G, Fu X, Adams J. Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1. J Mol Biol. 2008;376:55-68 pubmed
    ..The protein kinase responsible for this modification, SR-specific protein kinase 1 (SRPK1), catalyzes the selective phosphorylation of approximately a dozen serines in only the N-terminal portion of the ..
  47. Hagopian J, Ma C, Meade B, Albuquerque C, Ngo J, Ghosh G, et al. Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1. J Mol Biol. 2008;382:894-909 pubmed publisher
    ..To investigate how SRPK1 accomplishes this feat, the enzyme-substrate complex was analyzed using single-turnover and multiturnover kinetic ..
  48. Nousiainen L, Sillanpää M, Jiang M, Thompson J, Taipale J, Julkunen I. Human kinome analysis reveals novel kinases contributing to virus infection and retinoic-acid inducible gene I-induced type I and type III IFN gene expression. Innate Immun. 2013;19:516-30 pubmed publisher
    ..FYN oncogene related to SRC, FGR, YES), serine/threonine kinase 24, activin A receptor type 1 and SRPK1 (SFRS protein kinase 1)...
  49. Bullock N, Potts J, Simpkin A, Koupparis A, Harper S, Oxley J, et al. Serine-arginine protein kinase 1 (SRPK1), a determinant of angiogenesis, is upregulated in prostate cancer and correlates with disease stage and invasion. J Clin Pathol. 2016;69:171-5 pubmed publisher
    ..Preferential splicing of proangiogenic VEGF is determined by serine-arginine protein kinase 1 (SRPK1), which is upregulated in a number of cancers...
  50. Voukkalis N, Koutroumani M, Zarkadas C, Nikolakaki E, Vlassi M, Giannakouros T. SRPK1 and Akt Protein Kinases Phosphorylate the RS Domain of Lamin B Receptor with Distinct Specificity: A Combined Biochemical and In Silico Approach. PLoS ONE. 2016;11:e0154198 pubmed publisher
    ..we now demonstrate that while all serines of the RS domain represent more or less equal phosphoacceptor sites for SRPK1, Ser80 and Ser82 are mainly targeted by Akt...
  51. Mimura Y, Takagi M, Clever M, Imamoto N. ELYS regulates the localization of LBR by modulating its phosphorylation state. J Cell Sci. 2016;129:4200-4212 pubmed
    ..residues known to be phosphorylated by cyclin-dependent kinase (CDK) and serine/arginine protein kinases 1 and 2 (SRPK1 and SRPK2, respectively)...
  52. Wang H, Wang C, Tian W, Yao Y. The crucial role of SRPK1 in IGF-1-induced EMT of human gastric cancer. Oncotarget. 2017;8:72157-72166 pubmed publisher
    In recent years, the insulin-like growth factor (IGF-1) and serine-arginine protein kinase 1 (SRPK1) have been reported to be implicated in the pithelial-mesenchymal transition (EMT) in many kinds of malignancies...
  53. Sunatani Y, Kamdar R, Sharma M, Matsui T, Sakasai R, Hashimoto M, et al. Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase. Exp Cell Res. 2018;362:450-460 pubmed publisher
    ..This XRCC4-dependent, SRPK1/SRSF1-mediated regulatory mechanism was conserved in apoptosis in Jurkat human leukemia cells triggered by STS, ..
  54. Peidis P, Voukkalis N, Aggelidou E, Georgatsou E, Hadzopoulou Cladaras M, Scott R, et al. SAFB1 interacts with and suppresses the transcriptional activity of p53. FEBS Lett. 2011;585:78-84 pubmed publisher
    ..These data suggest that nuclear matrix-associated proteins may play a critical role in regulating p53 localization and activity. ..
  55. Amin E, Oltean S, Hua J, Gammons M, Hamdollah Zadeh M, Welsh G, et al. WT1 mutants reveal SRPK1 to be a downstream angiogenesis target by altering VEGF splicing. Cancer Cell. 2011;20:768-80 pubmed publisher
    ..cells, reduced VEGF(165)b was due to lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1. WT1 bound to the SRPK1 promoter, and repressed expression through a specific WT1 binding site...
  56. Lin J, Lin C, Tarn W, Li F. Elevated SRPK1 lessens apoptosis in breast cancer cells through RBM4-regulated splicing events. RNA. 2014;20:1621-31 pubmed publisher
    ..Elevated expression of serine-arginine protein kinase 1 (SRPK1) may participate in the pathway responsible for the dysregulation of splicing events in malignant tumor cells...
  57. Brown M, Bryant J, Dobrikova E, Shveygert M, Bradrick S, Chandramohan V, et al. Induction of viral, 7-methyl-guanosine cap-independent translation and oncolysis by mitogen-activated protein kinase-interacting kinase-mediated effects on the serine/arginine-rich protein kinase. J Virol. 2014;88:13135-48 pubmed publisher
    ..Therefore, it is critically important to understand the mechanisms controlling viral cap-independent translation in infected host cells. ..
  58. van Roosmalen W, Le Dévédec S, Golani O, Smid M, Pulyakhina I, Timmermans A, et al. Tumor cell migration screen identifies SRPK1 as breast cancer metastasis determinant. J Clin Invest. 2015;125:1648-64 pubmed publisher
    ..Examination of genes that modulate migration indicated that SRPK1, encoding the splicing factor kinase SRSF protein kinase 1, is relevant to breast cancer outcomes, as it was highly expressed in basal breast cancer...
  59. Bullock N, Oltean S. The many faces of SRPK1. J Pathol. 2017;241:437-440 pubmed publisher
    Serine-arginine protein kinase 1 (SRPK1) phosphorylates proteins involved in the regulation of several mRNA-processing pathways, including alternative splicing...
  60. Lee J, Jo Y, Kim M, Yoo N, Lee S. Inactivating frameshift mutation of putative tumor suppressor genes PLA2R1 and SRPK1 in gastric and colorectal cancers. Cancer Genet. 2017;210:34-35 pubmed publisher
  61. Wang H, Ge W, Jiang W, Li D, Ju X. SRPK1?siRNA suppresses K562 cell growth and induces apoptosis via the PARP?caspase3 pathway. Mol Med Rep. 2017;: pubmed publisher
    Serine-arginine protein kinase 1 (SRPK1) has been used as an important signal mediator, and is associated with cancer development...
  62. Velazquez Dones A, Hagopian J, Ma C, Zhong X, Zhou H, Ghosh G, et al. Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J Biol Chem. 2005;280:41761-8 pubmed
    ..We found that SRPK1 was responsible for efficient phosphorylation of a short stretch of amino acids in the N-terminal portion of the ..
  63. Ren G, Sheng L, Liu H, Sun Y, An Y, Li Y. The crucial role of SRPK1 in TGF-β-induced proliferation and apoptosis in the esophageal squamous cell carcinomas. Med Oncol. 2015;32:209 pubmed publisher
    In recent years, transforming growth factor-β (TGF-β) and the serine-arginine protein kinase 1 (SRPK1) have been recommended as a key signal mediator that is involved in oncogenesis...
  64. Umehara H, Nishii Y, Morishima M, Kakehi Y, Kioka N, Amachi T, et al. Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A. Biochem Biophys Res Commun. 2003;301:324-9 pubmed
    ..These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR proteins including CROP. ..
  65. Zhong X, Ding J, Adams J, Ghosh G, Fu X. Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones. Genes Dev. 2009;23:482-95 pubmed publisher
    ..Here, we report that SRPK1, a ubiquitously expressed SR protein-specific kinase, directly binds to the cochaperones Hsp40/DNAjc8 and Aha1, ..
  66. Huynh N, Ma C, Giang N, Hagopian J, Ngo J, Adams J, et al. Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1. Biochemistry. 2009;48:11432-40 pubmed publisher
    ..SR protein kinase 1 (SRPK1) phosphorylates approximately 12 of these serines using a semiprocessive mechanism...
  67. Sanidas I, Kotoula V, Ritou E, Daans J, Lenz C, Mairhofer M, et al. The ratio of SRPK1/SRPK1a regulates erythroid differentiation in K562 leukaemic cells. Biochim Biophys Acta. 2010;1803:1319-31 pubmed publisher
    b>SRPK1, the prototype of the serine/arginine family of kinases, has been implicated in the regulation of multiple cellular processes such as pre-mRNA splicing, chromatin structure, nuclear import and germ cell development...
  68. Edmond V, Moysan E, Khochbin S, Matthias P, Brambilla C, Brambilla E, et al. Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin. EMBO J. 2011;30:510-23 pubmed publisher
    ..addition, we show that Tip60 downregulates SRSF2 phosphorylation by inhibiting the nuclear translocation of both SRPK1 and SRPK2 kinases...
  69. Aubol B, Plocinik R, Hagopian J, Ma C, McGlone M, Bandyopadhyay R, et al. Partitioning RS domain phosphorylation in an SR protein through the CLK and SRPK protein kinases. J Mol Biol. 2013;425:2894-909 pubmed publisher
    ..two kinases partition Arg-Ser versus Ser-Pro specificities, we monitored the phosphorylation of SRSF1 by CLK1 and SRPK1. Although SRPK1 initially binds at the center of the RS domain phosphorylating in an orderly, N-terminal direction,..
  70. Keshwani M, Aubol B, Fattet L, Ma C, Qiu J, Jennings P, et al. Conserved proline-directed phosphorylation regulates SR protein conformation and splicing function. Biochem J. 2015;466:311-22 pubmed publisher
    ..Overall, these studies draw a direct causal relationship between a specific type of chemical modification in an SR protein and the regulation of alternative gene splicing programmes. ..
  71. Serrano P, Aubol B, Keshwani M, Forli S, Ma C, Dutta S, et al. Directional Phosphorylation and Nuclear Transport of the Splicing Factor SRSF1 Is Regulated by an RNA Recognition Motif. J Mol Biol. 2016;428:2430-2445 pubmed publisher
    ..These contacts provide a structural framework that balances the activities of SRPK1 and the protein phosphatase PP1, thereby regulating the phosphoryl content of the RS domain...
  72. Gong L, Song J, Lin X, Wei F, Zhang C, Wang Z, et al. Serine-arginine protein kinase 1 promotes a cancer stem cell-like phenotype through activation of Wnt/?-catenin signalling in NSCLC. J Pathol. 2016;240:184-96 pubmed publisher
    ..Herein, we showed that serine-arginine protein kinase 1 (SRPK1) was highly expressed at both the mRNA and the protein levels in human NCSLC...
  73. Aubol B, Wu G, Keshwani M, Movassat M, Fattet L, Hertel K, et al. Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing. Mol Cell. 2016;63:218-228 pubmed publisher
    ..CLK1 overcomes this dilemma through a symbiotic relationship with the serine-arginine protein kinase 1 (SRPK1)...
  74. Sigala I, Ganidis G, Thysiadis S, Zografos A, Giannakouros T, Sarli V, et al. Lynamicin D an antimicrobial natural product affects splicing by inducing the expression of SR protein kinase 1. Bioorg Med Chem. 2017;25:1622-1629 pubmed publisher
    ..We provide evidence that this effect is mainly due to the ability of lynamicin D to alter the levels of SRPK1, the key kinase involved in both constitutive and alternative splicing.
  75. Drakouli S, Lyberopoulou A, Papathanassiou M, Mylonis I, Georgatsou E. Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation. FEBS J. 2017;284:2482-2500 pubmed publisher, although it reverses the inhibition exerted by SAFB1/2 on the splicing kinase SR protein kinase 1 (SRPK1), which also binds on the C-terminus of SAFB1/2...
  76. Invergo B, Brochet M, Yu L, Choudhary J, Beltrao P, Billker O. Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation. Cell Rep. 2017;21:2017-2029 pubmed publisher
    ..the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1)...
  77. Papoutsopoulou S, Nikolakaki E, Chalepakis G, Kruft V, Chevaillier P, Giannakouros T. SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. Nucleic Acids Res. 1999;27:2972-80 pubmed
    ..TwoSRprotein-specifickinases, SRPK1 and SRPK2, have been considered as highly specific for the phosphorylation of these proteins, thereby contributing ..
  78. Kamachi M, Le T, Kim S, Geiger M, Anderson P, Utz P. Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway. J Exp Med. 2002;196:1213-25 pubmed
    ..phosphorylation of serine residues by at least eight different SR protein kinase kinases (SRPKs), including SRPK1, SRPK2, and the scleroderma autoantigen topoisomerase I...
  79. Ngo J, Gullingsrud J, Giang K, Yeh M, Fu X, Adams J, et al. SR protein kinase 1 is resilient to inactivation. Structure. 2007;15:123-33 pubmed
    SR protein kinase 1 (SRPK1) is a constitutively active kinase, which processively phosphorylates multiple serines within its substrates, ASF/SF2...
  80. Hayes G, Carrigan P, Miller L. Serine-arginine protein kinase 1 overexpression is associated with tumorigenic imbalance in mitogen-activated protein kinase pathways in breast, colonic, and pancreatic carcinomas. Cancer Res. 2007;67:2072-80 pubmed
    ..We have recently shown overexpression of a core splice regulatory protein, serine-arginine protein kinase 1 (SRPK1), in dysplastic and neoplastic pancreatic ductular cells...
  81. Dachsel J, Taylor J, Mok S, Ross O, Hinkle K, Bailey R, et al. Identification of potential protein interactors of Lrrk2. Parkinsonism Relat Disord. 2007;13:382-5 pubmed
    ..Future investigation of these candidates is now warranted and may help resolve the pathomechanism behind Lrrk2 neurodegeneration. ..
  82. Aubol B, Adams J. Applying the brakes to multisite SR protein phosphorylation: substrate-induced effects on the splicing kinase SRPK1. Biochemistry. 2011;50:6888-900 pubmed publisher
    ..The protein kinase SRPK1 phosphorylates ~10 serines in the arginine--serine-rich domain (RS domain) of the SR protein SRSF1 in a C- to N-..
  83. Sun L, Prince T, Manjarrez J, Scroggins B, Matts R. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta. 2012;1823:1092-101 pubmed publisher
    ..The proteins identified suggest that Aha1 may play roles in modulating RNA splicing and DNA repair, in addition to other cellular processes...
  84. Chang Y, Wu Q, Tian T, Li L, Guo X, Feng Z, et al. The influence of SRPK1 on glioma apoptosis, metastasis, and angiogenesis through the PI3K/Akt signaling pathway under normoxia. Tumour Biol. 2015;36:6083-93 pubmed publisher
    ..Serine/arginine SR protein kinases 1 (SRPK1) can highly and specifically phosphorylate the SR protein found in many tumors, which can influence cell ..
  85. Jamros M, Aubol B, Keshwani M, Zhang Z, Stamm S, Adams J. Intra-domain Cross-talk Regulates Serine-arginine Protein Kinase 1-dependent Phosphorylation and Splicing Function of Transformer 2β1. J Biol Chem. 2015;290:17269-81 pubmed publisher
    ..We now show that the serine-arginine protein kinase-1 (SRPK1) is a regulator of Tra2β1 and promotes exon inclusion in the survival motor neuron gene 2 (SMN2)...
  86. Zhang J, Jiang H, Xia W, Jiang Y, Tan X, Liu P, et al. Serine-arginine protein kinase 1 is associated with hepatocellular carcinoma progression and poor patient survival. Tumour Biol. 2016;37:283-90 pubmed publisher
    The pre-mRNA splicing regulator serine-arginine protein kinase 1 (SRPK1) promotes cancer development and various pathophysiological processes. However, the clinical relevance of SRPK1 in hepatocellular carcinoma (HCC) is not clear...
  87. Siqueira R, Barbosa Ã, Polêto M, Righetto G, Seraphim T, Salgado R, et al. Potential Antileukemia Effect and Structural Analyses of SRPK Inhibition by N-(2-(Piperidin-1-yl)-5-(Trifluoromethyl)Phenyl)Isonicotinamide (SRPIN340). PLoS ONE. 2015;10:e0134882 pubmed publisher
    ..Previous investigations have described the overexpression of SRPK1 and SRPK2 in leukemia and other cancer types, suggesting that they would be useful targets for developing novel ..
  88. Hu Z, Wang X, Guo W, Xie L, Huang Y, Liu Y, et al. Long non-coding RNA MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation in colorectal cancer cells. Oncotarget. 2016;7:11733-43 pubmed publisher
    ..We found that MALAT1 interacts with both SRPK1 and SRSF1. MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation...
  89. Munkley J, Livermore K, Rajan P, Elliott D. RNA splicing and splicing regulator changes in prostate cancer pathology. Hum Genet. 2017;136:1143-1154 pubmed publisher
    ..The splicing regulators Sam68 and Tra2β increase expression in prostate cancer. The SR protein kinase SRPK1 that modulates the activity of SR proteins is up-regulated in prostate cancer and has already given encouraging ..
  90. Paillard F. A novel soluble chimera for the treatment of familial hypercholesterolemia. Hum Gene Ther. 1999;10:1093-4 pubmed
  91. Daub H, Blencke S, Habenberger P, Kurtenbach A, Dennenmoser J, Wissing J, et al. Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. J Virol. 2002;76:8124-37 pubmed
    ..The 95-kDa kinase was purified and characterized as SR protein-specific kinase 1 (SRPK1) by mass spectrometry...
  92. Ma C, Hagopian J, Ghosh G, Fu X, Adams J. Regiospecific phosphorylation control of the SR protein ASF/SF2 by SRPK1. J Mol Biol. 2009;390:618-34 pubmed publisher
    ..While previous studies revealed that SRPK1 phosphorylates RS1, regiospecific and temporal-specific control within the largely redundant RS domain is not well ..