Gene Symbol: SPSB2
Description: splA/ryanodine receptor domain and SOCS box containing 2
Alias: GRCC9, SSB2, SPRY domain-containing SOCS box protein 2, SPRY domain-containing SOCS box protein SSB-2, gene-rich cluster protein C9
Species: human
Products:     SPSB2

Top Publications

  1. Wang D, Li Z, Messing E, Wu G. The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts with MET and enhances the hepatocyte growth factor-induced Erk-Elk-1-serum response element pathway. J Biol Chem. 2005;280:16393-401 pubmed
  2. Woo J, Imm J, Min C, Kim K, Cha S, Oh B. Structural and functional insights into the B30.2/SPRY domain. EMBO J. 2006;25:1353-63 pubmed
    ..2/SPRY domains, suggesting that these domains are protein-interacting modules, which recognize a specific individual partner protein rather than a consensus sequence motif. ..
  3. Zheng J, Chen J, Zou X, Zhao F, Guo M, Wang H, et al. Saikosaponin d causes apoptotic death of cultured neocortical neurons by increasing membrane permeability and elevating intracellular Ca2+ concentration. Neurotoxicology. 2019;70:112-121 pubmed publisher
    ..In the present study, we investigated the effect of five SSs (SSa, SSb1, SSb2, SSc, and SSd) on neuronal viability and the underlying mechanisms in cultured murine neocortical neurons...
  4. Zhao W, Cui H, Qiu K, Zhou T, Hong X, Liu X. Yeast molecular chaperone gene SSB2 is involved in the endoplasmic reticulum stress response. Antonie Van Leeuwenhoek. 2019;112:589-598 pubmed publisher
    The Saccharomyces cerevisiae chaperone gene SSB2 belongs to the Hsp70 family. Unlike other HSP70 genes, SSB2 gene expression is reduced after heat shock...
  5. Dong Z, Cheng F, Yuwen Y, Chen J, Li X, Dou H, et al. Identification and expression analysis of a Spsb gene in planarian Dugesia japonica. Gene. 2015;564:168-75 pubmed publisher
    ..that (1) DjSpsb is highly conserved in evolutionary history in metazoans and is at closer relationship to Spsb1, Spsb2 and Spsb4; (2) DjSpsb mRNA is mainly expressed in the head and also throughout head regeneration processes, ..
  6. Boucher D, Vu T, Bain A, Tagliaro Jahns M, Shi W, Lane S, et al. Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 2015;29:3326-34 pubmed publisher
    SSB1 and SSB2 are newly identified single-stranded (ss) DNA binding proteins that play a crucial role in genome maintenance in humans...
  7. Lin L, Chung C, Hsu W, Chang S, Hung T, Shields J, et al. Saikosaponin b2 is a naturally occurring terpenoid that efficiently inhibits hepatitis C virus entry. J Hepatol. 2015;62:541-8 pubmed publisher
    ..In a search for novel and economical prophylactic agents, we examined the antiviral activity of saikosaponins (SSa, SSb2, SSc, and SSd) from Bupleurum kaoi root (BK) as entry inhibitors against HCV infection...
  8. Chiabudini M, Tais A, Zhang Y, Hayashi S, Wölfle T, Fitzke E, et al. Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae. Mol Cell Biol. 2014;34:4062-76 pubmed publisher
    ..increased in the absence of the ribosome-bound chaperones ribosome-associated complex (RAC) and Ssb (Ssb1 and Ssb2)...
  9. Shin J, Im H, Lee K. Saikosaponin B2 Suppresses Inflammatory Responses Through IKK/IκBα/NF-κB Signaling Inactivation in LPS-Induced RAW 264.7 Macrophages. Inflammation. 2019;42:342-353 pubmed publisher
    ..falcatum, there is little information on bioactivity of saikosaponin B2 (SSB2)...

More Information


  1. Li Z, Sun H, Xing J, Qin X, Du G. Chemical and biological comparison of raw and vinegar-baked Radix Bupleuri. J Ethnopharmacol. 2015;165:20-8 pubmed publisher
    ..between the raw and the two processed RBs, and the vinegar-baking process induced elevated contents of ssb1, ssb2, acetic acid, malic acid, citric acid, 5-HMF, and ligustrazine, as well as the decreased contents of ssa, ssd, ..
  2. Gortmaker S, Long M, Resch S, Ward Z, Cradock A, Barrett J, et al. Cost Effectiveness of Childhood Obesity Interventions: Evidence and Methods for CHOICES. Am J Prev Med. 2015;49:102-11 pubmed publisher
    ..Cost-effectiveness evaluations of childhood obesity interventions can provide decision makers with information demonstrating best value for the money. ..
  3. Lee J, Crook N, Sun J, Alper H. Improvement of lactic acid production in Saccharomyces cerevisiae by a deletion of ssb1. J Ind Microbiol Biotechnol. 2016;43:87-96 pubmed publisher
    ..As SSB1 contains a close functional homolog SSB2 in yeast, this result was counterintuitive and may point to as-yet-undefined functional differences between SSB1 ..
  4. Guan X, Wang X, Yan K, Chu Y, Li S, Li W, et al. UFLC-MS/MS determination and pharmacokinetic studies of six Saikosaponins in rat plasma after oral administration of Bupleurum Dropping Pills. J Pharm Biomed Anal. 2016;124:288-293 pubmed publisher
    ..UFLC-MS/MS) was developed and validated for the simultaneous determination of six Saikosaponins (SSs) (SSa, SSb1, SSb2, SSd, SSc, SSf) of Bupleurum Dropping Pills (BDP) in rat plasma using chloramphenicol as the internal standard (IS)...
  5. Park S, Blanck H, Sherry B, Brener N, O Toole T. Factors associated with sugar-sweetened beverage intake among United States high school students. J Nutr. 2012;142:306-12 pubmed publisher
    ..Our findings of significant associations of high SSB intake with frequent fast-food restaurant use and sedentary behaviors may be used to tailor intervention efforts to reduce SSB intake among high-risk populations. ..
  6. Wang T, Luo S, Qin H, Xia Y. Hsp90 inhibition renders iNOS aggregation and the clearance of iNOS aggregates by proteasomes requires SPSB2. Free Radic Biol Med. 2018;117:90-98 pubmed publisher
    ..Further studies revealed that the SPRY domain-containing SOCS box protein 2 (SPSB2), an E3 ligase-recruiting protein, was essential for the ubiquitination of iNOS aggregates...
  7. Liu S, Tan Z, Li P, Gao X, Zeng Y, Wang S. HepG2 cells biospecific extraction and HPLC-ESI-MS analysis for screening potential antiatherosclerotic active components in Bupeuri radix. J Pharm Biomed Anal. 2016;121:56-62 pubmed publisher
    ..HepG2 cells, among these compounds, two potentially bioactive compounds namely saikosaponin b1 and saikosaponin b2 (SSb2) were identified by comparing with the chromatography of the standard sample and analysis of the structural ..
  8. Shi W, Vu T, Boucher D, Biernacka A, Nde J, Pandita R, et al. Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress. Blood. 2017;129:2479-2492 pubmed publisher
    ..The 2 single-stranded DNA (ssDNA) binding proteins SSB1 and SSB2 are crucial regulators of the DNA damage response; however, their overlapping roles during normal physiology are ..
  9. Bolt Evensen K, Vik F, Stea T, Klepp K, Bere E. Consumption of sugar-sweetened beverages and artificially sweetened beverages from childhood to adulthood in relation to socioeconomic status - 15 years follow-up in Norway. Int J Behav Nutr Phys Act. 2018;15:8 pubmed publisher
    ..Participants with high SES consumed in general less SSB (but not ASB), however, results varied depending on SES indicator used. The established inequalities persisted from childhood to adulthood. ..
  10. Wang S, Zhang Y, Zhang Q, Peng S, Shen C, Yu Y, et al. Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri. Environ Toxicol Pharmacol. 2017;52:129-137 pubmed publisher
    ..In parallel, none of SSc, SSb1, or SSb2 caused cell injury...
  11. Shearrer G, O Reilly G, Belcher B, Daniels M, Goran M, Spruijt Metz D, et al. The impact of sugar sweetened beverage intake on hunger and satiety in minority adolescents. Appetite. 2016;97:43-8 pubmed publisher
    ..86, CI=(-2.81, -0.92), p<0.01). Habitual SSB intake appears to play a key role in moderating fullness responses possibly via ghrelin. ..
  12. Krol M, Pawlowski K, Skierski J, Rao N, Hellmen E, Mol J, et al. Transcriptomic profile of two canine mammary cancer cell lines with different proliferative and anti-apoptotic potential. J Physiol Pharmacol. 2009;60 Suppl 1:95-106 pubmed
    ..expression of genes (at the level of transcripts) involved in Ca(2+) signaling pathway (Calmodulin 1, 2, 3 and SPSB2) and growth hormone cellular pathway...
  13. Matsumoto K, Nishiya T, Maekawa S, Horinouchi T, Ogasawara K, Uehara T, et al. The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetime of inducible nitric-oxide synthase. Biochem Biophys Res Commun. 2011;409:46-51 pubmed publisher
    ..These results demonstrate that ECS(SPSB), but not CHIP, is the master regulator of the iNOS lifetime. ..
  14. Ansari Lari M, Shen Y, Muzny D, Lee W, Gibbs R. Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination. Genome Res. 1997;7:268-80 pubmed
    ..The high number of genes with diverse functions and complex transcriptional patterns make this region ideal for addressing challenges of gene discovery and genomic characterization amenable to large-scale sequence analysis. ..
  15. Nishiya T, Matsumoto K, Maekawa S, Kajita E, Horinouchi T, Fujimuro M, et al. Regulation of inducible nitric-oxide synthase by the SPRY domain- and SOCS box-containing proteins. J Biol Chem. 2011;286:9009-19 pubmed publisher
    ..mechanisms of iNOS regulation, we have characterized the SPRY domain- and SOCS box-containing protein 1 (SPSB1), SPSB2, and SPSB4 that interact with the N-terminal region of iNOS in a D-I-N-N-N sequence-dependent manner...
  16. Ansari Lari M, Muzny D, Lu J, Lu F, Lilley C, Spanos S, et al. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13. Genome Res. 1996;6:314-26 pubmed
    ..Various computer software was utilized for analyses of the DNA and amino acid sequences. The results of the analyses and sequence-based strategies for gene identification are discussed. ..
  17. Kamura T, Maenaka K, Kotoshiba S, Matsumoto M, Kohda D, Conaway R, et al. VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases. Genes Dev. 2004;18:3055-65 pubmed
    ..These data suggest that the functions of the Cul2-Rbx1 and Cul5-Rbx2 modules are distinct. ..
  18. Kuang Z, Lewis R, Curtis J, Zhan Y, Saunders B, Babon J, et al. The SPRY domain-containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation. J Cell Biol. 2010;190:129-41 pubmed publisher
    ..We have identified SPRY domain-containing SOCS (suppressor of cytokine signaling) box protein 2 (SPSB2) as a novel negative regulator that recruits an E3 ubiquitin ligase complex to polyubiquitinate iNOS, resulting in ..
  19. Lievens S, Vanderroost N, Van der Heyden J, Gesellchen V, Vidal M, Tavernier J. Array MAPPIT: high-throughput interactome analysis in mammalian cells. J Proteome Res. 2009;8:877-86 pubmed publisher
    ..The array assay is an important addition to the MAPPIT suite of technologies that is expected to significantly increase its utility as a toolbox to screen for novel interactors of proteins or small molecules. ..
  20. You T, Wang Y, Li K, Zhang D, Wei H, Luo Y, et al. Crystal structure of SPSB2 in complex with a rational designed RGD-containing cyclic peptide inhibitor of SPSB2-iNOS interaction. Biochem Biophys Res Commun. 2017;489:346-352 pubmed publisher
    SPRY domain-containing SOCS box protein 2 (SPSB2) is a negative regulator of inducible nitric oxide synthase (iNOS) that modulates the lifetime of iNOS and thus the levels of nitric oxide (NO) production...
  21. Filippakopoulos P, Low A, Sharpe T, Uppenberg J, Yao S, Kuang Z, et al. Structural basis for Par-4 recognition by the SPRY domain- and SOCS box-containing proteins SPSB1, SPSB2, and SPSB4. J Mol Biol. 2010;401:389-402 pubmed publisher
    ..To further investigate this consensus motif, we determined the crystal structures of SPSB1, SPSB2, and SPSB4, as well as their binding modes and affinities for both Par-4 and VASA...
  22. Chittoor B, Krishnarjuna B, Morales R, MacRaild C, Sadek M, Leung E, et al. The Single Disulfide-Directed ?-Hairpin Fold. Dynamics, Stability, and Engineering. Biochemistry. 2017;56:2455-2466 pubmed publisher
    ..We show that sCon-Vc11-22[NNN12-14] binds to the iNOS-binding protein SPSB2 with an affinity of 1.3 ?M while maintaining the SDH fold...