SPOP

Summary

Gene Symbol: SPOP
Description: speckle-type POZ protein
Alias: BTBD32, TEF2, HIB homolog 1, roadkill homolog 1
Species: human

Top Publications

  1. pmc Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases
    Min Zhuang
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 36:39-50. 2009
  2. pmc Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancer
    Jiang Liu
    Institute for Genomics and Systems Biology, University of Chicago and Argonne National Laboratory, Chicago, IL 60637, USA
    Science 323:1218-22. 2009
  3. doi Coordinated activation of the nuclear ubiquitin ligase Cul3-SPOP by the generation of phosphatidylinositol 5-phosphate
    Matthew W Bunce
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    J Biol Chem 283:8678-86. 2008
  4. pmc Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase
    Inmaculada Hernández-Muñoz
    Division of Molecular Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 102:7635-40. 2005
  5. ncbi BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase
    Jeong Eun Kwon
    NRL of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 281:12664-72. 2006
  6. pmc The ubiquitin conjugating enzyme, UbcM2, engages in novel interactions with components of cullin-3 based E3 ligases
    Kendra S Plafker
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Biochemistry 48:3527-37. 2009
  7. pmc Insight in glioma susceptibility through an analysis of 6p22.3, 12p13.33-12.1, 17q22-23.2 and 18q23 SNP genotypes in familial and non-familial glioma
    Yanhong Liu
    Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Genet 131:1507-17. 2012
  8. ncbi In vivo topography of Rap1p-DNA complex at Saccharomyces cerevisiae TEF2 UAS(RPG) during transcriptional regulation
    Veronica De Sanctis
    Fondazione Istituto Pasteur Fondazione Cenci Bolognetti, c o Dipartimento di Genetica e Biologia Molecolare, Universita di Roma, La Sapienza, Rome, Italy
    J Mol Biol 318:333-49. 2002
  9. pmc Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genes
    Matthieu Le Gallo
    Cancer Genetics Branch, National Human Genome Research Institute, US National Institutes of Health NIH, Bethesda, MD, USA
    Nat Genet 44:1310-5. 2012
  10. pmc TFE2 and GATA3 enhance induction of POU4F3 and myosin VIIa positive cells in nonsensory cochlear epithelium by ATOH1
    Masatsugu Masuda
    Departments of Surgery Otolaryngology, La Jolla, CA 92093 0666, USA
    Dev Biol 372:68-80. 2012

Research Grants

Scientific Experts

  • Boohyeong Byun
  • R Stoehr
  • Ichiro Takahashi
  • Jan A K W Kiel
  • Igor A Valouev
  • Zhaoguo Tong
  • J Ao
  • Pao Tien Chuang
  • JEFFREY CHARLES RAUM
  • Yin Yin Choo
  • Qing Zhang
  • Christopher E Barbieri
  • Jeffrey D Singer
  • Jiang Liu
  • Daniel Nava Rodrigues
  • Zhi fang Wang
  • Vasileios Korakakis
  • Laura K van Geersdaele
  • Tyler Schwend
  • M Gyhagen
  • Min S Kim
  • Chuandong Geng
  • Mark A Rubin
  • Mirjam Blattner
  • Francesca Demichelis
  • Yuanyuan Xu
  • Yi Zhang
  • Matthieu Le Gallo
  • Yanhong Liu
  • Muhnho La
  • Wesley J Errington
  • Masatsugu Masuda
  • Fang Liu
  • Thilo Hagen
  • Sangita A Chakraborty
  • C Li
  • Bogyou Kim
  • Chengbing Wang
  • Xiaohui Wen
  • Kong Bung Choo
  • Kathryn C Claiborn
  • Dennis J van de Wijngaart
  • Min Zhuang
  • Miao Hsueh Chen
  • Kevin P White
  • Kendra S Plafker
  • Laurent Ruel
  • Cristina M Cummings
  • Sung Hee Baek
  • Koji Kasahara
  • Matthew W Bunce
  • Bing Wang
  • Jin Jiang
  • Doris A Stoffers
  • Lucia Di Marcotullio
  • Jeong Eun Kwon
  • David Kent
  • Cheol O Joe
  • Inmaculada Hernández-Muñoz
  • Aihua Liu
  • Daniel Shenton
  • Daniel D Billadeau
  • Lisa M Butler
  • David Lorente Estelles
  • Johann S de Bono
  • Andreas Diefenbach
  • Susan Gilfillan
  • Veronica De Sanctis
  • Nam J Yoo
  • Ji E Oh
  • Vijay Kumar Eedunuri
  • Gareth O Rosbrook
  • Sue Anne Chew
  • Richard Bond
  • BERT W O'MALLEY
  • Stephen B Carr
  • A N Jian-Mei
  • Jianhang Jia
  • Zhi Biao Wang
  • Helen J Close
  • Bin He
  • T F Nielsen
  • John Shou
  • - Wang-Wei
  • Mark A Stead
  • Simon D Connell
  • Zhigang Jin
  • Pengbo Zhou
  • Jian qiang Kong
  • Limei Xu

Detail Information

Publications66

  1. pmc Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases
    Min Zhuang
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 36:39-50. 2009
    ..Here, we present biochemical and structural analyses of the MATH-BTB protein, SPOP. We define a SPOP-binding consensus (SBC) and determine structures revealing recognition of SBCs from the ..
  2. pmc Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancer
    Jiang Liu
    Institute for Genomics and Systems Biology, University of Chicago and Argonne National Laboratory, Chicago, IL 60637, USA
    Science 323:1218-22. 2009
    ..Analysis of the model allowed the identification of a new role for the ubiquitin E3 ligase complex factor SPOP. In Drosophila, the gene encoding SPOP is a target of segmentation transcription factors...
  3. doi Coordinated activation of the nuclear ubiquitin ligase Cul3-SPOP by the generation of phosphatidylinositol 5-phosphate
    Matthew W Bunce
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    J Biol Chem 283:8678-86. 2008
    ..Using a yeast two-hybrid screen, we identified an interaction between the type IIbeta PIPK isoform (PIPKIIbeta) and SPOP (speckle-type POZ domain protein), a nuclear speckle-associated protein that recruits substrates to Cul3-based ..
  4. pmc Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase
    Inmaculada Hernández-Muñoz
    Division of Molecular Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 102:7635-40. 2005
    ..Here, we report that the E3 ubiquitin ligase consisting of SPOP and CULLIN3 is able to ubiquitinate the Polycomb group protein BMI1 and the variant histone MACROH2A...
  5. ncbi BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase
    Jeong Eun Kwon
    NRL of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 281:12664-72. 2006
    ..b>SPOP is a BTB (Bric-a-brac/Tramtrack/Broad complex) protein that constitutes Cul3-based ubiquitin ligases...
  6. pmc The ubiquitin conjugating enzyme, UbcM2, engages in novel interactions with components of cullin-3 based E3 ligases
    Kendra S Plafker
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Biochemistry 48:3527-37. 2009
    ..Together, these studies represent the first evidence that UbcM2, in concert with substrate adaptors, engages activated CUL3 ligases, thus suggesting that class III E2s are novel regulators of cullin ligases...
  7. pmc Insight in glioma susceptibility through an analysis of 6p22.3, 12p13.33-12.1, 17q22-23.2 and 18q23 SNP genotypes in familial and non-familial glioma
    Yanhong Liu
    Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Genet 131:1507-17. 2012
    ..33-12.1 PRMT8 rs17780102 (P = 0.031) and 17q12-21.32 SPOP rs650461 (P = 0.025)...
  8. ncbi In vivo topography of Rap1p-DNA complex at Saccharomyces cerevisiae TEF2 UAS(RPG) during transcriptional regulation
    Veronica De Sanctis
    Fondazione Istituto Pasteur Fondazione Cenci Bolognetti, c o Dipartimento di Genetica e Biologia Molecolare, Universita di Roma, La Sapienza, Rome, Italy
    J Mol Biol 318:333-49. 2002
    ..The implications of these results for the mechanism of Rap1p action are discussed...
  9. pmc Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genes
    Matthieu Le Gallo
    Cancer Genetics Branch, National Human Genome Research Institute, US National Institutes of Health NIH, Bethesda, MD, USA
    Nat Genet 44:1310-5. 2012
    ..identified high frequencies of somatic mutations in CHD4 (17%), EP300 (8%), ARID1A (6%), TSPYL2 (6%), FBXW7 (29%), SPOP (8%), MAP3K4 (6%) and ABCC9 (6%). Overall, 36...
  10. pmc TFE2 and GATA3 enhance induction of POU4F3 and myosin VIIa positive cells in nonsensory cochlear epithelium by ATOH1
    Masatsugu Masuda
    Departments of Surgery Otolaryngology, La Jolla, CA 92093 0666, USA
    Dev Biol 372:68-80. 2012
    ..of the greater epithelial ridge with multiple plasmids encoding human ATOH1 (hATOH1), hTCF3 (also known as E2A or TEF2), hGATA3, and hSP1. hATOH1 or hTCF3 alone induced Pou4f3/GFP cells but hGATA3 and hSP1 did not...
  11. doi FTH1 binds to Daxx and inhibits Daxx-mediated cell apoptosis
    Fang Liu
    Department of Clinical Laboratory, the First People s Hospital in Jiangbei District, Chongqing 400020, China
    Mol Biol Rep 39:873-9. 2012
    ..We present evidence to demonstrate the FTH1 and Daxx are able to participate in apoptosis pathway through JNK signal molecule and FTH1 can inhibit this pathway...
  12. doi Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR
    Yuanyuan Xu
    National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
    Biochem Biophys Res Commun 424:398-403. 2012
    ..RNA polymerase-II transcription factor (RPII), Elongation factor 1-b (EF-1b) and Translation elongation factor 2 (TEF2)...
  13. doi Mutational and expressional analyses of SPOP, a candidate tumor suppressor gene, in prostate, gastric and colorectal cancers
    Min S Kim
    Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea
    APMIS 121:626-33. 2013
    ..Speckle-type POZ protein (SPOP) is a key adaptor for Cul3-based ubiquitination process...
  14. pmc Mechanism of cullin3 E3 ubiquitin ligase dimerization
    Yin Yin Choo
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    PLoS ONE 7:e41350. 2012
    ..Our results provide evidence for BTB substrate receptor dependent Cul3 dimerization which is likely to play an important role in promoting substrate ubiquitination...
  15. pmc Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signaling
    Tyler Schwend
    From the Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois at Urbana Champaign, Urbana, Illinois 61802
    J Biol Chem 288:32809-20. 2013
    ..Here, we report that Dzip1 regulates Gli/Ci turnover by preventing degradation of speckle-type POZ protein (Spop), a protein that promotes proteasome-dependent turnover of Gli proteins...
  16. doi Structural basis of high-order oligomerization of the cullin-3 adaptor SPOP
    Laura K van Geersdaele
    School of Biology, University of Leeds, Leeds LS2 9JT, England
    Acta Crystallogr D Biol Crystallogr 69:1677-84. 2013
    ..b>SPOP is a BTB-domain adaptor of the cullin-3 E3 ligase complexes; it selectively recruits substrates via its N-terminal ..
  17. doi Spinal repositioning deficit. The effect of prolonged flexed posture
    Vasileios Korakakis
    Department of Physical Education and Sport Science, University of Thessaly, Trikala, Greece
    Br J Sports Med 47:e3. 2013
    ..active subjects were assessed regarding their habitual sitting posture (HSP), perception of optimal posture (SPOP), ability to adopt an instructed sitting posture (IOSP) and accuracy to reproduce the criterion posture immediately,..
  18. pmc Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae
    Daniel Shenton
    Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology UMIST, P O Box 88, Manchester M60 1QD, UK
    Biochem J 374:513-9. 2003
    ..a range of glycolytic and related enzymes (Tdh3, Eno2, Adh1, Tpi1, Ald6 and Fba1), as well as translation factors (Tef2, Tef5, Nip1 and Rps5) are identified...
  19. doi Molecular pathology and prostate cancer therapeutics: from biology to bedside
    Daniel Nava Rodrigues
    Prostate Cancer Targeted Therapy Group and Drug Development Unit, Royal Marsden NHS Foundation Trust and Institute of Cancer Research, Sutton, Surrey, UK
    J Pathol 232:178-84. 2014
    ..Tumours without this rearrangement include SPOP mutant as well as SPINK1-over-expressing subtypes...
  20. doi Prevalence and risk factors for pelvic organ prolapse 20 years after childbirth: a national cohort study in singleton primiparae after vaginal or caesarean delivery
    M Gyhagen
    Department of Obstetrics and Gynaecology, Sahlgrenska Academy at Gothenburg University, Gothenburg, Sweden
    BJOG 120:152-60. 2013
    To investigate prevalence and risk factors for symptomatic pelvic organ prolapse (sPOP) and sPOP concomitant with urinary incontinence (UI) in women 20 years after one vaginal delivery or one caesarean delivery.
  21. pmc Prostate cancer-associated mutations in speckle-type POZ protein (SPOP) regulate steroid receptor coactivator 3 protein turnover
    Chuandong Geng
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 110:6997-7002. 2013
    ..demonstrated that the E3 ubiquitin ligase adaptor speckle-type poxvirus and zinc finger (POZ) domain protein (SPOP) interacts directly with SRC-3 and promotes its cullin 3-dependent ubiquitination and proteolysis in breast cancer, ..
  22. ncbi [Construction and preliminary applications of a Saccharomyces cerevisiae detection plasmid using for screening promoter elements]
    Zhi fang Wang
    State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    Yao Xue Xue Bao 48:228-35. 2013
    ..The fluorescence microscopy results indicated that the six promoters (GAL1, GAL2, GAL7, GAL10, TEF2 and PGK1) can regulate the expression of DsRed-Monomer gene...
  23. pmc Single cell analysis of yeast replicative aging using a new generation of microfluidic device
    Yi Zhang
    The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Center for Quantitative Biology, and School of Physics, Peking University, China
    PLoS ONE 7:e48275. 2012
    ..throughout their lifespan, we discovered a surprising change of expression of the translation elongation factor TEF2 during aging, suggesting altered translational control in aged mother cells...
  24. pmc Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancer
    Christopher E Barbieri
    Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA
    Nat Genet 44:685-9. 2012
    ..New recurrent mutations were identified in multiple genes, including MED12 and FOXA1. SPOP was the most frequently mutated gene, with mutations involving the SPOP substrate-binding cleft in 6-15% of tumors ..
  25. doi Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase
    Wesley J Errington
    Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario M5S 1A8, Canada
    Structure 20:1141-53. 2012
    ..Here, we describe the dimeric structure of the E3 ligase adaptor protein SPOP (speckle-type POZ protein) in complex with the N-terminal domain of Cul3 at 2.4 Å resolution...
  26. doi Functional screening of FxxLF-like peptide motifs identifies SMARCD1/BAF60a as an androgen receptor cofactor that modulates TMPRSS2 expression
    Dennis J van de Wijngaart
    Department of Urology, Josephine Nefkens Institute, Erasmus Medical College, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Mol Endocrinol 23:1776-86. 2009
    ..In summary, our data demonstrate that BAF60a directly interacts with the coactivator groove in the AR ligand-binding domain via its FxxFF motif, thereby selectively activating specific AR-driven promoters...
  27. pmc Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation
    Xiaohui Wen
    Genentech, South San Francisco, CA 94080, USA
    Mol Cell Biol 30:1910-22. 2010
    ..to accumulation of full-length Gli3, but instead renders it labile, leading to its proteasomal degradation via the SPOP/Cul3 complex...
  28. doi Evolutionary expansion of SPOP and associated TD/POZ gene family: impact of evolutionary route on gene expression pattern
    Kong Bung Choo
    Department of Medical Research and Education, Taipei Veterans General Hospital, Shipai, Taipei, Taiwan
    Gene 460:39-47. 2010
    ..and -T1 (rT2 and rT1) retrogenes are members of the TD/POZ gene family which also includes the well-characterized SPOP gene...
  29. pmc Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligase
    Qing Zhang
    Departments of Developmental Biology and Pharmacology, University of Texas Southwestern Medical Center at Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 106:21191-6. 2009
    ..factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog-induced MATH and BTB domain-containing protein (HIB)/SPOP. Ci uses multiple Ser/Thr (S/T)-rich motifs that bind HIB cooperatively to mediate its degradation...
  30. pmc Suppressor of fused and Spop regulate the stability, processing and function of Gli2 and Gli3 full-length activators but not their repressors
    Chengbing Wang
    Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA
    Development 137:2001-9. 2010
    ..By contrast, RNAi knockdown of Spop (a substrate-binding adaptor for the cullin3-based ubiquitin E3 ligase) in Sufu mutant mouse embryonic fibroblasts (..
  31. pmc Pcif1 modulates Pdx1 protein stability and pancreatic β cell function and survival in mice
    Kathryn C Claiborn
    Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 120:3713-21. 2010
    ..Pdx1 C terminus-interacting factor-1 (Pcif1, also known as SPOP) is a nuclear protein that inhibits Pdx1 transactivation...
  32. pmc Tumor-suppressor role for the SPOP ubiquitin ligase in signal-dependent proteolysis of the oncogenic co-activator SRC-3/AIB1
    C Li
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA
    Oncogene 30:4350-64. 2011
    ..Here, we report that speckle-type POZ protein (SPOP), a cullin 3 (CUL3)-based ubiquitin ligase, is responsible for SRC-3 ubiquitination and proteolysis...
  33. pmc A single heterochromatin boundary element imposes position-independent antisilencing activity in Saccharomyces cerevisiae minichromosomes
    Sangita A Chakraborty
    Department of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Milton S Hershey Medical Center, Hershey, Pennsylvania, United States of America
    PLoS ONE 6:e24835. 2011
    ..multi-copy yeast minichromosome and we further showed that two distinct heterochromatin boundary elements STAR and TEF2-UASrpg are able to limit the heterochromatin spreading in circular minichromosomes...
  34. pmc Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity
    Yin Yin Choo
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117597 Singapore
    Mol Biol Cell 22:4706-15. 2011
    ..CSN also did not affect recruitment of the substrate-receptor SPOP to Cul3, suggesting it may not function to facilitate the exchange of Cul3 substrate receptors...
  35. doi Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex
    Bogyou Kim
    Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151 742, South Korea
    Biochem Biophys Res Commun 415:720-6. 2011
    ..ubiquitin ligase, is a new binding partner of BRMS1 and the interaction between BRMS1 and Cul3 is mediated by the SPOP adaptor protein...
  36. ncbi Identification of a novel nuclear speckle-type protein, SPOP
    Y Nagai
    Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University, Japan
    FEBS Lett 418:23-6. 1997
    ..No zinc finger or RNA recognition motifs were found in this clone. The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined...
  37. pmc Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved
    Miao Hsueh Chen
    Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California 94158, USA
    Genes Dev 23:1910-28. 2009
    ..Consistent with this, Sufu regulates Gli protein levels by antagonizing the activity of Spop, a conserved Gli-degrading factor...
  38. pmc Variations in Hedgehog signaling: divergence and perpetuation in Sufu regulation of Gli
    Laurent Ruel
    Institut Biologie du Développement et Cancer IBDC, Universite de Nice Sophia Antipolis, CNRS UMR 6543, Centre de Biochimie, Nice Cedex 02, France
    Genes Dev 23:1843-8. 2009
    ..1910-1928) demonstrate that mouse Suppressor of fused (Sufu) regulates the stability of the transcription factors Gli2 and Gli3 by antagonizing the conserved Gli degradation device mediated by Hib/Spop in a cilia-independent manner.
  39. ncbi MacroH2A1.2 binds the nuclear protein Spop
    Ichiro Takahashi
    Division of Genetic Resources, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Biochim Biophys Acta 1591:63-8. 2002
    ..2. The results showed that macroH2A1.2 interacts with mouse nuclear speckled type protein Spop. The Spop protein has a unique composition: an N-terminal MATH, and a C-terminal BTB/POZ domain...
  40. ncbi A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains
    J M Zapata
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:24242-52. 2001
    ..MUL, the product of the causative gene in Mulibrey Nanism syndrome; USP7 (HAUSP), an ubiquitin protease; and SPOP, a POZ domain-containing protein...
  41. ncbi PCR-based gene targeting in the filamentous fungus Ashbya gossypii
    J Wendland
    , Biozentrum, University of Basel, Switzerland
    Gene 242:381-91. 2000
    ..Escherichia coli kanR gene under the control of promoter and terminator sequences of the Saccharomyces cerevisiae TEF2 gene and allows selection of G418/geneticin-resistant transformants...
  42. ncbi Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae
    S U Aström
    Department of Microbiology, Umea University, Sweden
    Mol Gen Genet 261:967-76. 1999
    ..On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1alpha (eEF-1alpha), into rit1 null strains with two intact ..
  43. ncbi An activating immunoreceptor complex formed by NKG2D and DAP10
    J Wu
    DNAX Research Institute, 901 California Avenue, Palo Alto, CA 94304, USA
    Science 285:730-2. 1999
    ..Thus, NKG2D-DAP10 receptor complexes may activate NK and T cell responses against MICA-bearing tumors...
  44. pmc The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11
    J I Piruat
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
    Genetics 147:1585-94. 1997
    ..Constitutive transcription was not affected, as determined in ADH1 and TEF2. In addition, Hrs1p was required for mating-factor expression, telomere-linked DNA silencing and DNA supercoiling ..
  45. pmc Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta
    T G Kinzy
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Genetics 141:481-9. 1995
    ..a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha...
  46. pmc GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domain
    J Tornow
    Department of Biological Sciences, University of Southern Mississippi, Hattiesburg 39406 5018
    EMBO J 12:2431-7. 1993
    ..GCR1-dependent transcription of either the glycolytic gene ADH1 or the translational component genes TEF1 and TEF2. We have also identified an activation domain within a segment of the GCR1 protein (the N-terminal third) that is ..
  47. ncbi Cloning and genetic characterization of a calcium- and phospholipid-binding protein from Saccharomyces cerevisiae that is homologous to translation elongation factor-1 gamma
    N G Kambouris
    Department of Pharmacology, University of Virginia, Charlottesville 22903
    Yeast 9:151-63. 1993
    ..No evidence was found for an interaction of CAM1 with TEF1, TEF2, SEC4, YPT1, RAS1, RAS2, CDC6, ARF1, ARF2 or CIN4...
  48. ncbi Either one of the two yeast EF-1 alpha genes is required for cell viability
    P Cottrelle
    Service de Biochimie, C E N SACLAY, Gif sur Yvette, France
    Curr Genet 9:693-7. 1985
    Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae...
  49. pmc Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites
    G M Santangelo
    Department of Biology, Portland State University, Oregon 97201 0751
    Mol Cell Biol 10:859-62. 1990
    ..We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption...
  50. ncbi Efficient expression of the Saccharomyces cerevisiae glycolytic gene ADH1 is dependent upon a cis-acting regulatory element (UASRPG) found initially in genes encoding ribosomal proteins
    J Tornow
    Biology Department, Portland State University, OR 97201 0751
    Gene 90:79-85. 1990
    ..In band retardation assays, the promoters for the elongation factor 1 alpha-encoding genes (TEF1 and TEF2) competed for binding of the protein to the copy of UASRPG in the ADH1 promoter...
  51. ncbi NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation
    Susan Gilfillan
    Department of Pathology and Immunology, Washington University School of Medicine, 660 S Euclid, St Louis, MO 63110, USA
    Nat Immunol 3:1150-5. 2002
    ..Thus, NKG2D is a versatile receptor that, depending on the availability of adapter partners, mediates costimulation in T cells and/or activation in NK cells...
  52. ncbi Selective associations with signaling proteins determine stimulatory versus costimulatory activity of NKG2D
    Andreas Diefenbach
    Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720 3200, USA
    Nat Immunol 3:1142-9. 2002
    ..This strategy suggests a rationale for the multisubunit structure of stimulatory immunoreceptors...
  53. ncbi NKG2D-DAP10 triggers human NK cell-mediated killing via a Syk-independent regulatory pathway
    Daniel D Billadeau
    Division of Oncology Research, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nat Immunol 4:557-64. 2003
    ..Yet the signals initiated by NKG2D-DAP10 are fully capable of inducing killing. Our findings identify a previously unknown mechanism by which receptor complexes that lack ITAM motifs can trigger lymphocyte activation...
  54. pmc Selection of reliable reference genes for gene expression studies in peach using real-time PCR
    Zhaoguo Tong
    College of Horticulture, Nanjing Agricultural University, 1 Tongwei Road, Weigang, Nanjing 210095, PR China
    BMC Mol Biol 10:71. 2009
    ..Only a few studies on reference genes have been done in plants and none in peach (Prunus persica L. Batsch). Therefore, the present study was conducted to identify suitable reference gene(s) for normalization of gene expression in peach...
  55. pmc Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast
    Igor A Valouev
    Cardiology Research Center, 121552 Moscow, Russia
    BMC Mol Biol 10:60. 2009
    ..Besides termination, both release factors have essential, but poorly characterized functions outside of translation...
  56. pmc The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cells
    Cristina M Cummings
    Department of Molecular Biology, Cell Biology and Biochemistry and the Center for Genomics and Proteomics, Brown University, Providence, Rhode Island 02903, USA
    J Biol Chem 284:11663-75. 2009
    ..We demonstrate a novel regulatory mechanism for p60/katanin that occurs at the level of targeted proteolysis to allow normal mitotic progression in mammalian cells...
  57. ncbi BTB/POZ domain of speckle-type POZ protein (SPOP) confers proapoptotic function in HeLa cells
    Boohyeong Byun
    Department of Oriental Medicine, Daegu Haany University, Daegu, South Korea
    Biofactors 31:165-9. 2007
    The proapoptotic function of SPOP protein was investigated in HeLa cells. HeLa cells underwent apoptosis by the overexpression of SPOP...
  58. ncbi Repression of transcriptional activity of estrogen receptor alpha by a Cullin3/SPOP ubiquitin E3 ligase complex
    Boohyeong Byun
    Department of Oriental Medicine, Daegu Haany University, Daegu 705 060, Korea
    Mol Cells 25:289-93. 2008
    The role of SPOP in the ubiquitination of ER alpha by the Cullin3-based E3 ubiquitin ligase complex was investigated...
  59. pmc Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II
    Koji Kasahara
    Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, 230 0045, Japan
    Nucleic Acids Res 36:1343-57. 2008
    ..upstream shift in transcriptional start sites of RPS5, RPS16A, RPL23B, RPL27B and RPL32, but not of RPS31, RPL10, TEF2 and ADH1, indicating that HMO1 may participate in start site selection of a subset of class II genes presumably via ..
  60. ncbi Overproduction of translation elongation factor 1-alpha (eEF1A) suppresses the peroxisome biogenesis defect in a Hansenula polymorpha pex3 mutant via translational read-through
    Jan A K W Kiel
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, The Netherlands
    FEMS Yeast Res 7:1114-25. 2007
    ..We have isolated two genes (TEF1 and TEF2) that encode eEF1A, and which are constitutively expressed...
  61. ncbi Multiple ubiquitin-dependent processing pathways regulate hedgehog/gli signaling: implications for cell development and tumorigenesis
    Lucia Di Marcotullio
    Department of Experimental Medicine and Pathology, Cenci Bolognetti Foundation, University La Sapienza, Roma, Italy
    Cell Cycle 6:390-3. 2007
    ..Cullin family-based ubiquitination results in either Cullin1-Slimb/betaTrCP- or Cullin3-HIB/Roadkill/SPOP-dependent proteolytic processing or degradation of Drosophila Cubitus interruptus or mammalian Gli proteins...
  62. ncbi A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor
    Qing Zhang
    Center for Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    Dev Cell 10:719-29. 2006
    ..The mammalian homolog of HIB, SPOP, can functionally substitute for HIB, and Gli proteins are degraded by HIB/SPOP in Drosophila...
  63. ncbi Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus
    David Kent
    Department of Cell and Developmental Biology, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA
    Development 133:2001-10. 2006
    ..The existence of human orthologs for Rdx raises the possibility that this novel feedback loop also modulates Hh responses in humans...
  64. ncbi Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP
    Muhnho La
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305 701, Republic of Korea
    Biochem Biophys Res Commun 320:760-5. 2004
    Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient...
  65. pmc Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activity
    Aihua Liu
    Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, and Penn Diabetes Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 24:4372-83. 2004
    ..We have identified a novel POZ domain protein, PDX-1 C terminus-interacting factor 1 (PCIF1)/SPOP, that interacts with PDX-1 both in vitro and in vivo...
  66. pmc Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae
    M A Huie
    Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville 32610 0266
    Mol Cell Biol 12:2690-700. 1992
    ..detect specific interactions with CTTCC sequence elements found in front of the translational component genes TEF1, TEF2, and CRY1...

Research Grants3

  1. Molecular Mechanisms of Mammalian Hedgehog Signal Transduction
    Pao Tien Chuang; Fiscal Year: 2010
    ..3) Delineate the requirement of primary cilia in Sufu- or SPOP-mediated Gli protein function...