Genomes and Genes
Gene Symbol: SPOP
Description: speckle-type POZ protein
Alias: BTBD32, TEF2, HIB homolog 1, roadkill homolog 1
- Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligaseWesley J Errington
Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario M5S 1A8, Canada
Structure 20:1141-53. 2012..Here, we describe the dimeric structure of the E3 ligase adaptor protein SPOP (speckle-type POZ protein) in complex with the N-terminal domain of Cul3 at 2.4 Å resolution...
- Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancerChristopher E Barbieri
Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA
Nat Genet 44:685-9. 2012..New recurrent mutations were identified in multiple genes, including MED12 and FOXA1. SPOP was the most frequently mutated gene, with mutations involving the SPOP substrate-binding cleft in 6-15% of tumors ..
- Variations in Hedgehog signaling: divergence and perpetuation in Sufu regulation of GliLaurent Ruel
Institut Biologie du Développement et Cancer IBDC, Universite de Nice Sophia Antipolis, CNRS UMR 6543, Centre de Biochimie, Nice Cedex 02, France
Genes Dev 23:1843-8. 2009..1910-1928) demonstrate that mouse Suppressor of fused (Sufu) regulates the stability of the transcription factors Gli2 and Gli3 by antagonizing the conserved Gli degradation device mediated by Hib/Spop in a cilia-independent manner.
- Selection of reliable reference genes for gene expression studies in peach using real-time PCRZhaoguo Tong
College of Horticulture, Nanjing Agricultural University, 1 Tongwei Road, Weigang, Nanjing 210095, PR China
BMC Mol Biol 10:71. 2009..Only a few studies on reference genes have been done in plants and none in peach (Prunus persica L. Batsch). Therefore, the present study was conducted to identify suitable reference gene(s) for normalization of gene expression in peach...
- Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeastIgor A Valouev
Cardiology Research Center, 121552 Moscow, Russia
BMC Mol Biol 10:60. 2009..Besides termination, both release factors have essential, but poorly characterized functions outside of translation...
- The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cellsCristina M Cummings
Department of Molecular Biology, Cell Biology and Biochemistry and the Center for Genomics and Proteomics, Brown University, Providence, Rhode Island 02903, USA
J Biol Chem 284:11663-75. 2009..We demonstrate a novel regulatory mechanism for p60/katanin that occurs at the level of targeted proteolysis to allow normal mitotic progression in mammalian cells...
- Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancerJiang Liu
Institute for Genomics and Systems Biology, University of Chicago and Argonne National Laboratory, Chicago, IL 60637, USA
Science 323:1218-22. 2009..Analysis of the model allowed the identification of a new role for the ubiquitin E3 ligase complex factor SPOP. In Drosophila, the gene encoding SPOP is a target of segmentation transcription factors...
- BTB/POZ domain of speckle-type POZ protein (SPOP) confers proapoptotic function in HeLa cellsBoohyeong Byun
Department of Oriental Medicine, Daegu Haany University, Daegu, South Korea
Biofactors 31:165-9. 2007The proapoptotic function of SPOP protein was investigated in HeLa cells. HeLa cells underwent apoptosis by the overexpression of SPOP...
- Repression of transcriptional activity of estrogen receptor alpha by a Cullin3/SPOP ubiquitin E3 ligase complexBoohyeong Byun
Department of Oriental Medicine, Daegu Haany University, Daegu 705 060, Korea
Mol Cells 25:289-93. 2008The role of SPOP in the ubiquitination of ER alpha by the Cullin3-based E3 ubiquitin ligase complex was investigated...
- Coordinated activation of the nuclear ubiquitin ligase Cul3-SPOP by the generation of phosphatidylinositol 5-phosphateMatthew W Bunce
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
J Biol Chem 283:8678-86. 2008..Using a yeast two-hybrid screen, we identified an interaction between the type IIbeta PIPK isoform (PIPKIIbeta) and SPOP (speckle-type POZ domain protein), a nuclear speckle-associated protein that recruits substrates to Cul3-based ..
- Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase IIKoji Kasahara
Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, 230 0045, Japan
Nucleic Acids Res 36:1343-57. 2008..upstream shift in transcriptional start sites of RPS5, RPS16A, RPL23B, RPL27B and RPL32, but not of RPS31, RPL10, TEF2 and ADH1, indicating that HMO1 may participate in start site selection of a subset of class II genes presumably via ..
- Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conservedMiao Hsueh Chen
Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California 94158, USA
Genes Dev 23:1910-28. 2009..Consistent with this, Sufu regulates Gli protein levels by antagonizing the activity of Spop, a conserved Gli-degrading factor...
- Functional screening of FxxLF-like peptide motifs identifies SMARCD1/BAF60a as an androgen receptor cofactor that modulates TMPRSS2 expressionDennis J van de Wijngaart
Department of Urology, Josephine Nefkens Institute, Erasmus Medical College, P O Box 2040, 3000 CA Rotterdam, The Netherlands
Mol Endocrinol 23:1776-86. 2009..In summary, our data demonstrate that BAF60a directly interacts with the coactivator groove in the AR ligand-binding domain via its FxxFF motif, thereby selectively activating specific AR-driven promoters...
- Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligasesMin Zhuang
Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Mol Cell 36:39-50. 2009..Here, we present biochemical and structural analyses of the MATH-BTB protein, SPOP. We define a SPOP-binding consensus (SBC) and determine structures revealing recognition of SBCs from the ..
- Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complexBogyou Kim
Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151 742, South Korea
Biochem Biophys Res Commun 415:720-6. 2011..ubiquitin ligase, is a new binding partner of BRMS1 and the interaction between BRMS1 and Cul3 is mediated by the SPOP adaptor protein...
- Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activityYin Yin Choo
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117597 Singapore
Mol Biol Cell 22:4706-15. 2011..CSN also did not affect recruitment of the substrate-receptor SPOP to Cul3, suggesting it may not function to facilitate the exchange of Cul3 substrate receptors...
- A single heterochromatin boundary element imposes position-independent antisilencing activity in Saccharomyces cerevisiae minichromosomesSangita A Chakraborty
Department of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Milton S Hershey Medical Center, Hershey, Pennsylvania, United States of America
PLoS ONE 6:e24835. 2011..multi-copy yeast minichromosome and we further showed that two distinct heterochromatin boundary elements STAR and TEF2-UASrpg are able to limit the heterochromatin spreading in circular minichromosomes...
- Tumor-suppressor role for the SPOP ubiquitin ligase in signal-dependent proteolysis of the oncogenic co-activator SRC-3/AIB1C Li
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA
Oncogene 30:4350-64. 2011..Here, we report that speckle-type POZ protein (SPOP), a cullin 3 (CUL3)-based ubiquitin ligase, is responsible for SRC-3 ubiquitination and proteolysis...
- Pcif1 modulates Pdx1 protein stability and pancreatic β cell function and survival in miceKathryn C Claiborn
Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Clin Invest 120:3713-21. 2010..Pdx1 C terminus-interacting factor-1 (Pcif1, also known as SPOP) is a nuclear protein that inhibits Pdx1 transactivation...
- Suppressor of fused and Spop regulate the stability, processing and function of Gli2 and Gli3 full-length activators but not their repressorsChengbing Wang
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA
Development 137:2001-9. 2010..By contrast, RNAi knockdown of Spop (a substrate-binding adaptor for the cullin3-based ubiquitin E3 ligase) in Sufu mutant mouse embryonic fibroblasts (..
- Evolutionary expansion of SPOP and associated TD/POZ gene family: impact of evolutionary route on gene expression patternKong Bung Choo
Department of Medical Research and Education, Taipei Veterans General Hospital, Shipai, Taipei, Taiwan
Gene 460:39-47. 2010..and -T1 (rT2 and rT1) retrogenes are members of the TD/POZ gene family which also includes the well-characterized SPOP gene...
- Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradationXiaohui Wen
Genentech, South San Francisco, CA 94080, USA
Mol Cell Biol 30:1910-22. 2010..to accumulation of full-length Gli3, but instead renders it labile, leading to its proteasomal degradation via the SPOP/Cul3 complex...
- Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligaseQing Zhang
Departments of Developmental Biology and Pharmacology, University of Texas Southwestern Medical Center at Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 106:21191-6. 2009..factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog-induced MATH and BTB domain-containing protein (HIB)/SPOP. Ci uses multiple Ser/Thr (S/T)-rich motifs that bind HIB cooperatively to mediate its degradation...
- Overproduction of translation elongation factor 1-alpha (eEF1A) suppresses the peroxisome biogenesis defect in a Hansenula polymorpha pex3 mutant via translational read-throughJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, The Netherlands
FEMS Yeast Res 7:1114-25. 2007..We have isolated two genes (TEF1 and TEF2) that encode eEF1A, and which are constitutively expressed...
- Multiple ubiquitin-dependent processing pathways regulate hedgehog/gli signaling: implications for cell development and tumorigenesisLucia Di Marcotullio
Department of Experimental Medicine and Pathology, Cenci Bolognetti Foundation, University La Sapienza, Roma, Italy
Cell Cycle 6:390-3. 2007..Cullin family-based ubiquitination results in either Cullin1-Slimb/betaTrCP- or Cullin3-HIB/Roadkill/SPOP-dependent proteolytic processing or degradation of Drosophila Cubitus interruptus or mammalian Gli proteins...
- Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiaeS U Aström
Department of Microbiology, Umea University, Sweden
Mol Gen Genet 261:967-76. 1999..On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1alpha (eEF-1alpha), into rit1 null strains with two intact ..
- An activating immunoreceptor complex formed by NKG2D and DAP10J Wu
DNAX Research Institute, 901 California Avenue, Palo Alto, CA 94304, USA
Science 285:730-2. 1999..Thus, NKG2D-DAP10 receptor complexes may activate NK and T cell responses against MICA-bearing tumors...
- Identification of a novel nuclear speckle-type protein, SPOPY Nagai
Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University, Japan
FEBS Lett 418:23-6. 1997..No zinc finger or RNA recognition motifs were found in this clone. The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined...
- The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11J I Piruat
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
Genetics 147:1585-94. 1997..Constitutive transcription was not affected, as determined in ADH1 and TEF2. In addition, Hrs1p was required for mating-factor expression, telomere-linked DNA silencing and DNA supercoiling ..
- Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 betaT G Kinzy
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
Genetics 141:481-9. 1995..a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha...
- GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domainJ Tornow
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg 39406 5018
EMBO J 12:2431-7. 1993..GCR1-dependent transcription of either the glycolytic gene ADH1 or the translational component genes TEF1 and TEF2. We have also identified an activation domain within a segment of the GCR1 protein (the N-terminal third) that is ..
- Cloning and genetic characterization of a calcium- and phospholipid-binding protein from Saccharomyces cerevisiae that is homologous to translation elongation factor-1 gammaN G Kambouris
Department of Pharmacology, University of Virginia, Charlottesville 22903
Yeast 9:151-63. 1993..No evidence was found for an interaction of CAM1 with TEF1, TEF2, SEC4, YPT1, RAS1, RAS2, CDC6, ARF1, ARF2 or CIN4...
- Either one of the two yeast EF-1 alpha genes is required for cell viabilityP Cottrelle
Service de Biochimie, C E N SACLAY, Gif sur Yvette, France
Curr Genet 9:693-7. 1985Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae...
- Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sitesG M Santangelo
Department of Biology, Portland State University, Oregon 97201 0751
Mol Cell Biol 10:859-62. 1990..We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption...
- Efficient expression of the Saccharomyces cerevisiae glycolytic gene ADH1 is dependent upon a cis-acting regulatory element (UASRPG) found initially in genes encoding ribosomal proteinsJ Tornow
Biology Department, Portland State University, OR 97201 0751
Gene 90:79-85. 1990..In band retardation assays, the promoters for the elongation factor 1 alpha-encoding genes (TEF1 and TEF2) competed for binding of the protein to the copy of UASRPG in the ADH1 promoter...
- PCR-based gene targeting in the filamentous fungus Ashbya gossypiiJ Wendland
, Biozentrum, University of Basel, Switzerland
Gene 242:381-91. 2000..Escherichia coli kanR gene under the control of promoter and terminator sequences of the Saccharomyces cerevisiae TEF2 gene and allows selection of G418/geneticin-resistant transformants...
- A diverse family of proteins containing tumor necrosis factor receptor-associated factor domainsJ M Zapata
Burnham Institute, La Jolla, California 92037, USA
J Biol Chem 276:24242-52. 2001..MUL, the product of the causative gene in Mulibrey Nanism syndrome; USP7 (HAUSP), an ubiquitin protease; and SPOP, a POZ domain-containing protein...
- A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factorQing Zhang
Center for Developmental Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
Dev Cell 10:719-29. 2006..The mammalian homolog of HIB, SPOP, can functionally substitute for HIB, and Gli proteins are degraded by HIB/SPOP in Drosophila...
- Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptusDavid Kent
Department of Cell and Developmental Biology, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA
Development 133:2001-10. 2006..The existence of human orthologs for Rdx raises the possibility that this novel feedback loop also modulates Hh responses in humans...
- BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligaseJeong Eun Kwon
NRL of Protein Biochemistry, School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
J Biol Chem 281:12664-72. 2006..b>SPOP is a BTB (Bric-a-brac/Tramtrack/Broad complex) protein that constitutes Cul3-based ubiquitin ligases...
- Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligaseInmaculada Hernández-Muñoz
Division of Molecular Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
Proc Natl Acad Sci U S A 102:7635-40. 2005..Here, we report that the E3 ubiquitin ligase consisting of SPOP and CULLIN3 is able to ubiquitinate the Polycomb group protein BMI1 and the variant histone MACROH2A...
- Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOPMuhnho La
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305 701, Republic of Korea
Biochem Biophys Res Commun 320:760-5. 2004Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient...
- Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activityAihua Liu
Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, and Penn Diabetes Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 24:4372-83. 2004..We have identified a novel POZ domain protein, PDX-1 C terminus-interacting factor 1 (PCIF1)/SPOP, that interacts with PDX-1 both in vitro and in vivo...
- NKG2D-DAP10 triggers human NK cell-mediated killing via a Syk-independent regulatory pathwayDaniel D Billadeau
Division of Oncology Research, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, Minnesota 55905, USA
Nat Immunol 4:557-64. 2003..Yet the signals initiated by NKG2D-DAP10 are fully capable of inducing killing. Our findings identify a previously unknown mechanism by which receptor complexes that lack ITAM motifs can trigger lymphocyte activation...
- Selective associations with signaling proteins determine stimulatory versus costimulatory activity of NKG2DAndreas Diefenbach
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720 3200, USA
Nat Immunol 3:1142-9. 2002..This strategy suggests a rationale for the multisubunit structure of stimulatory immunoreceptors...
- NKG2D recruits two distinct adapters to trigger NK cell activation and costimulationSusan Gilfillan
Department of Pathology and Immunology, Washington University School of Medicine, 660 S Euclid, St Louis, MO 63110, USA
Nat Immunol 3:1150-5. 2002..Thus, NKG2D is a versatile receptor that, depending on the availability of adapter partners, mediates costimulation in T cells and/or activation in NK cells...
- MacroH2A1.2 binds the nuclear protein SpopIchiro Takahashi
Division of Genetic Resources, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
Biochim Biophys Acta 1591:63-8. 2002..2. The results showed that macroH2A1.2 interacts with mouse nuclear speckled type protein Spop. The Spop protein has a unique composition: an N-terminal MATH, and a C-terminal BTB/POZ domain...
- Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiaeM A Huie
Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville 32610 0266
Mol Cell Biol 12:2690-700. 1992..detect specific interactions with CTTCC sequence elements found in front of the translational component genes TEF1, TEF2, and CRY1...
- Molecular Mechanisms of Mammalian Hedgehog Signal TransductionPao Tien Chuang; Fiscal Year: 2010..3) Delineate the requirement of primary cilia in Sufu- or SPOP-mediated Gli protein function...