SPF45

Summary

Gene Symbol: SPF45
Description: RNA binding motif protein 17
Alias: SPF45, 45 kDa-splicing factor, RNA-binding motif protein 17, splicing factor 45, splicing factor 45kDa
Species: human

Top Publications

  1. ncbi Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45
    María José Lallena
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Cell 109:285-96. 2002
  2. ncbi Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
  3. ncbi Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetes
    Christopher E Lowe
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, UK
    Nat Genet 39:1074-82. 2007
  4. ncbi Human splicing factor SPF45 (RBM17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators
    William L Perry
    Lilly Research Laboratories, Eli Lilly and Co, Indianapolis, Indiana 46285, USA
    Cancer Res 65:6593-600. 2005
  5. ncbi Drosophila SPF45: a bifunctional protein with roles in both splicing and DNA repair
    Ahmad Sami Chaouki
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS Genet 2:e178. 2006
  6. ncbi U2AF-homology motif interactions are required for alternative splicing regulation by SPF45
    Lorenzo Corsini
    European Molecular Biology Laboratory EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 14:620-9. 2007
  7. ncbi Human SPF45, a splicing factor, has limited expression in normal tissues, is overexpressed in many tumors, and can confer a multidrug-resistant phenotype to cells
    Janardhan Sampath
    Cancer Research Division, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Am J Pathol 163:1781-90. 2003
  8. ncbi Sex, AGility, and the regulation of alternative splicing
    Brenton R Graveley
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington 06030, USA
    Cell 109:409-12. 2002
  9. ncbi Holliday junction-binding activity of human SPF45
    Naoki Horikoshi
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Genes Cells 15:373-83. 2010
  10. ncbi Variant of TYR and autoimmunity susceptibility loci in generalized vitiligo
    Ying Jin
    Human Medical Genetics Program, School of Medicine, University of Colorado, P O Box 6511, Mailstop 8300, Aurora, CO 80045, USA
    N Engl J Med 362:1686-97. 2010

Research Grants

  1. Regulation of Ovarian Cancer Multidrug Resistance by MAP Kinases
    Scott T Eblen; Fiscal Year: 2010
  2. MOLECULAR STUDIES OF SPINOCEREBELLAR ATAXIA TYPE 1
    Huda Y Zoghbi; Fiscal Year: 2010
  3. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
  4. Alternative Splicing of the Drosophila DSCAM Pre-DRNA
    Brenton R Graveley; Fiscal Year: 2010
  5. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
  6. Alternative Splicing of the Drosophila DSCAM Pre-DRNA
    BRENTON GRAVELEY; Fiscal Year: 2007
  7. THE FUNCTION OF PRE-MRNA SPLICING ACTIVATORS
    BRENTON GRAVELEY; Fiscal Year: 2005
  8. Alternative Splicing of the Drosophila Dscam Pre-mRNA
    BRENTON GRAVELEY; Fiscal Year: 2006

Scientific Experts

  • BRENTON GRAVELEY
  • William L Perry
  • J D Cooper
  • Huda Y Zoghbi
  • Scott T Eblen
  • SCOTT EBLEN
  • Shaojuan Lai
  • Naoki Horikoshi
  • Ying Jin
  • Harry T Orr
  • Janghoo Lim
  • Lorenzo Corsini
  • Christopher E Lowe
  • Ahmad Sami Chaouki
  • Juan Valcarcel
  • Janardhan Sampath
  • María José Lallena
  • Brennon O'Callaghan
  • E Helen Kemp
  • Nanny van Geel
  • Mauro Picardo
  • Andreas Overbeck
  • Alexandra J D Sufit
  • Anthony P Weetman
  • Stanca A Birlea
  • Paulene J Holland
  • Thomas Jouary
  • Jo Lambert
  • Hitoshi Kurumizaka
  • Dorothy C Bennett
  • Saunie M Hutton
  • Katherine Gowan
  • Motoki Takaku
  • David J Gawkrodger
  • Khaled Ezzedine
  • Pamela R Fain
  • Wayne T McCormack
  • Richard A Spritz
  • Christina M Mailloux
  • Sheri L Riccardi
  • Giovanni Leone
  • Anita Amadi-Myers
  • Alain Taieb
  • Yoshimasa Takizawa
  • Yuichi Morozumi
  • Margaret R Wallace
  • Aaron B Bowman
  • Paymaan Jafar-Nejad
  • Ronald Richman
  • Juan Crespo-Barreto
  • David E Hill
  • Klaus Scheffzek
  • Todd Brusko
  • Sophie Bonnal
  • Michael Sattler
  • Kirsi Bourget
  • Deborah J Smyth
  • Michael Hothorn
  • David G Clayton
  • Vincent Plagnol
  • Rebecca Bailey
  • John A Todd
  • Sophie Bonna
  • Linda S Wicker
  • Neil M Walker
  • Mark Atkinson
  • Sarah Field
  • Jerome Basquin
  • Helen K Salz
  • Anne H Dantzig
  • Mark Williamson
  • Robert E Moore
  • Viswanath Devanarayan
  • Xiaoling Xia
  • Mark Rolfe
  • George E Sandusky
  • Robert L Shepard
  • Pandy R Long
  • Kevin J Chalmers
  • Salud Llamazares
  • Angus I Lamond

Detail Information

Publications12

  1. ncbi Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45
    María José Lallena
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Cell 109:285-96. 2002
    ..Here we show that U2AF interacts with the Py-tract and downstream AG, whereas the spliceosomal protein SPF45 interacts with the upstream AG and activates it for the second catalytic step of the splicing reaction...
  2. ncbi Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
    ..This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases...
  3. ncbi Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetes
    Christopher E Lowe
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, UK
    Nat Genet 39:1074-82. 2007
    ..635). Furthermore, we have associated IL2RA T1D susceptibility genotypes with lower circulating levels of the biomarker, soluble IL-2RA (P = 6.28 x 10(-28)), suggesting that an inherited lower immune responsiveness predisposes to T1D...
  4. ncbi Human splicing factor SPF45 (RBM17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators
    William L Perry
    Lilly Research Laboratories, Eli Lilly and Co, Indianapolis, Indiana 46285, USA
    Cancer Res 65:6593-600. 2005
    The splicing factor SPF45 (RBM17) is frequently overexpressed in many solid tumors, and stable expression in HeLa cells confers resistance to doxorubicin and vincristine...
  5. ncbi Drosophila SPF45: a bifunctional protein with roles in both splicing and DNA repair
    Ahmad Sami Chaouki
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS Genet 2:e178. 2006
    The sequence of the SPF45 protein is significantly conserved, yet functional studies have identified it as a splicing factor in animal cells and as a DNA-repair protein in plants...
  6. ncbi U2AF-homology motif interactions are required for alternative splicing regulation by SPF45
    Lorenzo Corsini
    European Molecular Biology Laboratory EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 14:620-9. 2007
    ..Here we report that the splicing factor SPF45 regulates alternative splicing of the apoptosis regulatory gene FAS (also called CD95)...
  7. ncbi Human SPF45, a splicing factor, has limited expression in normal tissues, is overexpressed in many tumors, and can confer a multidrug-resistant phenotype to cells
    Janardhan Sampath
    Cancer Research Division, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Am J Pathol 163:1781-90. 2003
    ..novel drug-resistant genes in cyclophosphamide-resistant EMT6 mouse mammary tumors led us to the identification of SPF45. Simultaneously, other groups identified SPF45 as a component of the spliceosome that is involved in alternative ..
  8. ncbi Sex, AGility, and the regulation of alternative splicing
    Brenton R Graveley
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington 06030, USA
    Cell 109:409-12. 2002
    ..detailed examination of the Drosophila Sex-lethal gene has led to two significant discoveries-the role of the splicing factor SPF45 in defining the site of exon ligation, and that alternative splicing can be regulated at the second step.
  9. ncbi Holliday junction-binding activity of human SPF45
    Naoki Horikoshi
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Genes Cells 15:373-83. 2010
    b>SPF45 is considered to be a bifunctional protein that functions in splicing and DNA repair...
  10. ncbi Variant of TYR and autoimmunity susceptibility loci in generalized vitiligo
    Ying Jin
    Human Medical Genetics Program, School of Medicine, University of Colorado, P O Box 6511, Mailstop 8300, Aurora, CO 80045, USA
    N Engl J Med 362:1686-97. 2010
    ..Generalized vitiligo is an autoimmune disease characterized by melanocyte loss, which results in patchy depigmentation of skin and hair, and is associated with an elevated risk of other autoimmune diseases...
  11. ncbi 14-3-3 Binding to ataxin-1(ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus
    Shaojuan Lai
    Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 286:34606-16. 2011
    ..In the nucleus, we propose that dephosphorylation of pS776-ATXN1 by PP2A regulates the interaction of ATXN1 with the splicing factors RBM17 and U2AF65...
  12. ncbi Meta-analysis of genome-wide association study data identifies additional type 1 diabetes risk loci
    Jason D Cooper
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Nat Genet 40:1399-401. 2008
    ..7 x 10(-12)), 10p15 (PRKCQ, P = 3.7 x 10(-9)), 15q24 (CTSH, P = 3.2 x 10(-15)) and 22q13 (C1QTNF6, P = 2.0 x 10(-8))...

Research Grants21

  1. Regulation of Ovarian Cancer Multidrug Resistance by MAP Kinases
    Scott T Eblen; Fiscal Year: 2010
    ..Over-expression of the splicing factor SPF45 in ovarian cancer cells results in a tamoxifen sensitive drug-resistant phenotype...
  2. MOLECULAR STUDIES OF SPINOCEREBELLAR ATAXIA TYPE 1
    Huda Y Zoghbi; Fiscal Year: 2010
    ....
  3. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
    ....
  4. Alternative Splicing of the Drosophila DSCAM Pre-DRNA
    Brenton R Graveley; Fiscal Year: 2010
    ....
  5. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
    ....
  6. Alternative Splicing of the Drosophila DSCAM Pre-DRNA
    BRENTON GRAVELEY; Fiscal Year: 2007
    ....
  7. THE FUNCTION OF PRE-MRNA SPLICING ACTIVATORS
    BRENTON GRAVELEY; Fiscal Year: 2005
    ..In addition, at least 35 percent of human genes are alternatively spliced. Our results will increase our understanding of how this important means of gene regulation is controlled. ..
  8. Alternative Splicing of the Drosophila Dscam Pre-mRNA
    BRENTON GRAVELEY; Fiscal Year: 2006
    ....