Sp2

Summary

Gene Symbol: Sp2
Description: Sp2 transcription factor
Alias: transcription factor Sp2
Species: human
Products:     Sp2

Top Publications

  1. Kalayu Yirga S, Ling S, Yang Y, Yuan J, Wang S. The Preparation and Identification of a Monoclonal Antibody against Citrinin and the Development of Detection via Indirect Competitive ELISA. Toxins (Basel). 2017;9: pubmed publisher
    ..Mice were immunized with CTN-BSA conjugates, and spleen cells of the immunized mice were fused with Sp2/0 myeloma cells to obtain 21H27 hybriodoma cell...
  2. Ling S, Xiao S, Xie C, Wang R, Zeng L, Wang K, et al. Preparation of Monoclonal Antibody for Brevetoxin 1 and Development of Ic-ELISA and Colloidal Gold Strip to Detect Brevetoxin 1. Toxins (Basel). 2018;10: pubmed publisher
    ..A hybridoma cell line 6C6 stably secreting monoclonal antibody (mAb) against BTX-1 was obtained by fusing SP2/0 myeloma cells with the spleen cells from the immunized mouse...
  3. Ghaderi F, Ahmadvand S, Ramezani A, Montazer M, Ghaderi A. Production and characterization of monoclonal antibody against a triple negative breast cancer cell line. Biochem Biophys Res Commun. 2018;505:181-186 pubmed publisher
    ..cell line, established in Shiraz Institute for Cancer Research) were fused with the mouse myeloma cell line SP2/0 in the presence of polyethylene glycol...
  4. Dai H, Zhang H, Wang M, Ge F, Shen Y. [Preparation and characterization of monoclonal antibodies against human neuropilin 1 b1b2 domain (HuNRP1b)]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:1278-1283 pubmed
    ..Then His-HuNRP1b was used as immunogen to immune BALB/c mice. After the fusion of spleen cells and Sp2/0 cells, the positive hybridoma cells were screened by indirect ELISA that was established with recombinant HuNRP1<..
  5. Saeed A, Ling S, Yuan J, Wang S. The Preparation and Identification of a Monoclonal Antibody against Domoic Acid and Establishment of Detection by Indirect Competitive ELISA. Toxins (Basel). 2017;9: pubmed publisher
    ..DA-BSA conjugate was used as immunogen and DA-OVA as coating antigen. Cell fusion between spleen cells and sp2/0 myeloma cells developed 1C3 hybridoma clone producing 1C3 monoclonal antibody (mAb)...
  6. Liu L. Antibody glycosylation and its impact on the pharmacokinetics and pharmacodynamics of monoclonal antibodies and Fc-fusion proteins. J Pharm Sci. 2015;104:1866-1884 pubmed publisher
    ..Glycans can also have impacts on the safety of mAb. mAbs produced in murine myeloma cells such as NS0 and SP2/0 contain glycans such as Galα1-3Galβ1-4N-acetylglucosamine-R and N-glycolylneuraminic acid (NGNA) that are ..
  7. Zhang Y, Cao P, Lu F, Yan X, Jiang B, Cheng J, et al. Generation of Monoclonal Antibodies Against Natural Products. J Vis Exp. 2019;: pubmed publisher
    ..antibody titer and fused with the hypoxanthine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line Sp2/0 -Ag14 using a polyethylene glycol (PEG)-based method...
  8. Ahirwar M, Kataktalware M, Pushpadass H, Jeyakumar S, Jash S, Nazar S, et al. Scrotal infrared digital thermography predicts effects of thermal stress on buffalo (Bubalus bubalis) semen. J Therm Biol. 2018;78:51-57 pubmed publisher
    ..Thermographic images were analyzed with Quick Report 1.2 SP2 software and temperature data acquired...
  9. Zhou Y, Xu Z, Yang L, Chen X, Duan Z, Wen J. [Preparation of mouse monoclonal antibodies against EV71 capsid protein VP1]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018;34:462-468 pubmed
    ..Recombinant VP1 was used to immunize BALB/c mice; the splenocytes were fused with myeloma SP2/0 cell line and the hybridoma cells which could produce VP1-specific mAbs were screened using ELISA; the hybridoma ..

More Information

Publications133 found, 100 shown here

  1. Zhang S, Luo X, Guo A, Zhu X, Cai X. Generation and characterization of monoclonal antibodies specific for 18 kDa antigen from Taenia solium cysticerci. Exp Parasitol. 2016;166:75-82 pubmed publisher
    ..solium cysticerci (TsVF), with mouse myeloma cells (SP2/0). The reactivity and specificity of these MAbs were evaluated by indirect ELISA and immunoblotting techniques...
  2. Gabriel G, Viviani V. Novel application of pH-sensitive firefly luciferases as dual reporter genes for simultaneous ratiometric analysis of intracellular pH and gene expression/location. Photochem Photobiol Sci. 2014;13:1661-70 pubmed publisher
    ..red regions of the bioluminescence spectra and pH using firefly luciferases cloned in our laboratory (Macrolampis sp2 and Cratomorphus distinctus), allowing estimation of E...
  3. Chen Y, Zhang C. Preparation of Antispermidine/Spermine-N1-Acetyltransferase Monoclonal Antibodies. Monoclon Antib Immunodiagn Immunother. 2016;35:163-6 pubmed publisher
    ..multifocal intradermal injections and by fusing high-titer antibody-producing spleen cells with myeloma cells of SP2/0 origin. Four mAbs were selected for further characterization as classes and subclasses...
  4. Kong X, Xu P, Cai W, Wang H, Li B, Huang G, et al. ZBP-89 and Sp1 contribute to Bak expression in hepatocellular carcinoma cells. BMC Cancer. 2018;18:419 pubmed publisher
    ..level was correlated with Sp1 and Sp3 expression level, while no correlation was found in ZBP-89 and Bak, neither Sp2 nor Sp4. Mithramycin A (MMA) induced Bak expression in a dose-dependent manner...
  5. Trabbic K, Kleski K, Shi M, Bourgault J, Prendergast J, Dransfield D, et al. Production of a mouse monoclonal IgM antibody that targets the carbohydrate Thomsen-nouveau cancer antigen resulting in in vivo and in vitro tumor killing. Cancer Immunol Immunother. 2018;67:1437-1447 pubmed publisher
    ..from Tn-PS A1 hyperimmunized Jackson Laboratory C57BL/6 mice, splenocytes and the murine myeloma cell line Sp2/0Ag14 with subsequent cloning on methyl cellulose semi-solid media...
  6. Liu Y, Zhang Y, Sun X, Liu S. Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis. Genet Mol Res. 2016;15: pubmed publisher
    ..BALB/c mice were immunized to prepare the anti-JSRV-Env572-615 mAb. Spleen cells were fused with SP2/0 myeloma cells after being screened by indirect ELISA and cloned by limiting dilution...
  7. Maier M, Reusch D, Bruggink C, Bulau P, Wuhrer M, Mølhøj M. Applying mini-bore HPAEC-MS/MS for the characterization and quantification of Fc N-glycans from heterogeneously glycosylated IgGs. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1033-1034:342-352 pubmed publisher
    ..mixtures of N-glycans derived from immunoglobulin G from human plasma, glyco-engineered CHO cells, and Sp2/0 mouse myeloma cells. Compared to an analytical scale 3mm I.D...
  8. De Clare Bronsvoort B, von Wissmann B, Fèvre E, Handel I, Picozzi K, Welburn S. No gold standard estimation of the sensitivity and specificity of two molecular diagnostic protocols for Trypanosoma brucei spp. in Western Kenya. PLoS ONE. 2010;5:e8628 pubmed publisher
    ..760) had a higher sensitivity than the ITS-PCR (Se2=0.640); both have high specificity (Sp1=0.998; Sp2=0.997). The true prevalences for livestock populations were estimated (pcattle=0.091, ppigs=0.066, pgoats=0...
  9. Schalchli H, Hormazábal E, Rubilar O, Briceño G, Mutis A, Zocolo G, et al. Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source. J Appl Microbiol. 2017;123:886-895 pubmed publisher
    ..b>Sp2 showed the highest inhibition zones of Mucor miehei...
  10. Sugiaman Trapman D, Vitezic M, Jouhilahti E, Mathelier A, Lauter G, Misra S, et al. Characterization of the human RFX transcription factor family by regulatory and target gene analysis. BMC Genomics. 2018;19:181 pubmed publisher
    ..We uncovered and experimentally confirmed the TFs SP2 and ESR1 as upstream regulators of specific RFX genes...
  11. Yan X, Wu L, Du X, Li J, Zhang F, Han Y, et al. [Preparation and application of monoclonal antibodies against DR region of Na+-K+-ATPase ?1 subunit]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1695-1699 pubmed
    ..Splenocytes from the immunized mice were collected and subsequently fused with SP2/0 mouse myeloma cells. Positive hybridoma clones were obtained after cell fusion and selection...
  12. Li Q, Chen H, Li Z, Shen P, Qu L, Gong L, et al. Preparation and characterization of anti-GP73 monoclonal antibodies and development of double-antibody sandwich ELISA. Asian Pac J Cancer Prev. 2015;16:2043-9 pubmed
    ..Two hybridoma cell lines against GP73 were obtained by fusing mouse Sp2/0 myeloma cells with spleen cells from the immunized mice. The titers of anti-GP73 mAb reached 1:243,000...
  13. O Reilly L, Cullen L, Moriishi K, O CONNOR L, Huang D, Strasser A. Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins. Biotechniques. 1998;25:824-30 pubmed
    ..The supernatants were derived from a fusion of Sp2/0 plasmacytoma cells and spleen cells from a rat immunized with recombinant glutathione-S-transferase (GST)-BimL ..
  14. Maroto Morales A, Ramón M, García Álvarez O, Montoro V, Soler A, Fernández Santos M, et al. Sperm head phenotype and male fertility in ram semen. Theriogenology. 2015;84:1536-41 pubmed publisher
    ..results show the existence of four sperm SPs, which present different sperm head phenotype: SP1 (large and round), SP2 (short and elongated), SP3 (shortest and round), and SP4 (large and the most elongated)...
  15. Jin N, Ling S, Yang C, Wang S. Preparation and identification of monoclonal antibody against Citreoviridin and development of detection by Ic-ELISA. Toxicon. 2014;90:226-36 pubmed publisher
    ..A hybridoma cell line 8D8 stably secreting monoclonal antibody against CIT was generated by fusing SP2/0 myeloma cells with the splenocytes from the immunized mice. The titer of 8D8 mAb reached 1: 1...
  16. Wasserthal L, Cloetens P, Fink R, Wasserthal L. X-ray computed tomography study of the flight-adapted tracheal system in the blowfly Calliphora vicina, analysing the ventilation mechanism and flow-directing valves. J Exp Biol. 2018;221: pubmed publisher
    ..airflow is directed by two valves that open alternately: (1) the hinged filter flaps of the metathoracic spiracles (Sp2) are passively pushed open during the upstroke by the increased tracheal pressure, thereby enabling expiration; (2) ..
  17. Dorado J, Cid R, Molina A, Hidalgo M, Ariza J, Moreno Millán M, et al. Effect of inbreeding depression on bull sperm quality and field fertility. Reprod Fertil Dev. 2017;29:712-720 pubmed publisher
    ..05). Cluster analysis of kinematic parameters revealed three different sperm subpopulations (sP1, sP2 and sP3), with the proportion of the sP1 population (highly active but non-progressive spermatozoa) being ..
  18. Tomiyama H, Nishikimi T, Matsumoto C, Kimura K, Odaira M, Shiina K, et al. Longitudinal changes in late systolic cardiac load and serum NT-proBNP levels in healthy middle-aged Japanese men. Am J Hypertens. 2015;28:452-8 pubmed publisher
    ..the radial pressure waveform (SP1), systolic and pulse pressure at the second peak of the radial pressure waveform (SP2 and PP2), and serum levels of N-terminal fragment B-type natriuretic peptide (NT-proBNP) were measured at the start ..
  19. Lazarus D, Weinstein Marom H, Fishman S, Yossef R, Zuri D, Barnea E, et al. Efficient peptide recovery from secreted recombinant MHC-I molecules expressed via mRNA transfection. Immunol Lett. 2015;165:32-8 pubmed publisher
    ..express soluble derivatives of HLA-A2 in the human AF10 B cell myeloma and 624mel melanoma and H-2K(d) in the mouse SP2/0 B cell myeloma...
  20. Mikulska J. Analysis of Response Elements Involved in the Regulation of the Human Neonatal Fc Receptor Gene (FCGRT). PLoS ONE. 2015;10:e0135141 pubmed publisher
    ..identified binding motifs in the hFCGRT promoter were able to specifically interact with their corresponding (Sp1, Sp2, Sp3, c-Fos, c-Jun, YY1, and C/EBPbeta or C/EBPdelta) transcription factors (TFs), suggesting their possible ..
  21. Wang R, Zeng L, Yang H, Zhong Y, Wang J, Ling S, et al. Detection of okadaic acid (OA) using ELISA and colloidal gold immunoassay based on monoclonal antibody. J Hazard Mater. 2017;339:154-160 pubmed publisher
    ..In this study, a monoclonal antibody named 10E8 was screened by cells fusion of Sp2/0 with spleen cells isolated from immunized mouse, and the isotype of McAb 10E8 was belonged to IgG1...
  22. Kuo H, Hsu Y, Wu M, Woon P, Wong H, Tsai L, et al. FCGR2A Promoter Methylation and Risks for Intravenous Immunoglobulin Treatment Responses in Kawasaki Disease. Mediators Inflamm. 2015;2015:564625 pubmed publisher
    ..In addition, CpG sites G, J, and K were predicted as transcription factors binding sites for NF-kB, Myc-Max, and SP2, respectively...
  23. Begum Z, Varalakshmi C, Sriram D, Radha V. Development and characterization of a novel monoclonal antibody that recognizes an epitope in the central protein interaction domain of RapGEF1 (C3G). Mol Biol Rep. 2018;45:1809-1819 pubmed publisher
    ..having only the Crk binding region of RapGEF1 and hybridoma clones created by fusion of immunized spleen cells with Sp2/0 myeloma cells. This antibody recognizes human, primate and murine RapGEF1 protein...
  24. Narayan J, Bhaumik A, Sachan R, Haque A, Gupta S, Pant P. Direct conversion of carbon nanofibers and nanotubes into diamond nanofibers and the subsequent growth of large-sized diamonds. Nanoscale. 2019;11:2238-2248 pubmed publisher
    ..electron microscopy with a characteristic peak at 292 eV for σ* for sp3 bonding and the absence of π* for sp2 bonding...
  25. Zhou Y, Ma Z, Tang J, Yan N, Du Y, Xi S, et al. Immediate hydroxylation of arenes to phenols via V-containing all-silica ZSM-22 zeolite triggered non-radical mechanism. Nat Commun. 2018;9:2931 pubmed publisher
    Hydroxylation of arenes via activation of aromatic Csp2-H bond has attracted great attention for decades but remains a huge challenge...
  26. Vásquez F, Soler C, Camps P, Valverde A, García Molina A. Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele. Asian J Androl. 2016;18:824-830 pubmed publisher
    ..For head size, the entire sperm population could be divided into two morphometric subpopulations, SP1 and SP2, which did not change during adolescence...
  27. Tatzber F, Pursch E, Resch U, Pfragner R, Holasek S, Lindschinger M, et al. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxid Med Cell Longev. 2017;2017:6047142 pubmed publisher
    ..In our study, human B-cells from the peripheral blood of a healthy female donor were fused with the SP2/0 mouse myeloma cell line...
  28. Limsuwanchote S, Wungsintaweekul J, Keawpradub N, Putalun W, Morimoto S, Tanaka H. Development of indirect competitive ELISA for quantification of mitragynine in Kratom (Mitragyna speciosa (Roxb.) Korth.). Forensic Sci Int. 2014;244:70-7 pubmed publisher
    ..Hybridoma 1A6 that was constructed from the fusion between splenocytes of EDC/NHS conjugate immunized mice and SP2/0-Ag14 myeloma cells was selected, cloned twice and expanded...
  29. De Caroli M, Lenucci M, Manualdi F, Dalessandro G, De Lorenzo G, Piro G. Molecular dissection of Phaseolus vulgaris polygalacturonase-inhibiting protein 2 reveals the presence of hold/release domains affecting protein trafficking toward the cell wall. Front Plant Sci. 2015;6:660 pubmed publisher
    ..Its depletion leads, in fact, to the secretion via default (Sp2-susceptible) of the ACD-GFP chimera faster than PGIP2...
  30. Li G, Zhao L, Zhou F, Li J, Xing Y, Wang T, et al. Monoclonal antibody production and indirect competitive enzyme-linked immunosorbent assay development of 3-methyl-quinoxaline-2-carboxylic acid based on novel haptens. Food Chem. 2016;209:279-85 pubmed publisher
    ..Female BALB/c mice were immunized with above conjugates, splenocytes were fused with Sp2/0 cells to produce monoclonal antibody...
  31. Wang X, Zhao Y, Li L, Cheng Z, Wang G. Preparation and Application of a Monoclonal Antibody Against Chicken TRIM62. Monoclon Antib Immunodiagn Immunother. 2018;37:134-138 pubmed publisher
    ..The hybridomas were obtained by fusing mouse myeloma cell line SP2/0 with splenocytes of immunized mice...
  32. Viger M, Smith H, Cohen D, Dewoody J, Trewin H, Steenackers M, et al. Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar (Populus nigra L.). Tree Physiol. 2016;36:909-28 pubmed publisher
    ..Transcriptome analyses of a genotype from Spain (Sp2, originating from an arid site) and another from northern Italy (Ita, originating from a wet site) revealed ..
  33. Borhani K, Bamdad T, Hashempour T. Lenalidomide acts as an adjuvant for HCV DNA vaccine. Int Immunopharmacol. 2017;48:231-240 pubmed publisher
    ..Also, results of the therapeutic mice model, in which SP2/0 cells- challenged mice were treated with 5mg/kg lenalidomide in combination with pcDNA3...
  34. Yee W. Microalgae from the Selenastraceae as emerging candidates for biodiesel production: a mini review. World J Microbiol Biotechnol. 2016;32:64 pubmed publisher
    ..Strains from the Selenastraceae such as Monoraphidium sp. FXY-10, M. contortum SAG 47.80, Ankistrodesmus sp. SP2-15 and M. minutum were high biomass and lipid producers when cultivated under optimal conditions...
  35. Macinnes M, Hlynchuk S, Acharya S, Lehnert N, Maldonado S. Reduction of Graphene Oxide Thin Films by Cobaltocene and Decamethylcobaltocene. ACS Appl Mater Interfaces. 2017;: pubmed publisher
    ..solutions of cobaltocene & trifluoroacetic acid were most effective at reducing the material to sp2 carbon...
  36. Cui B, Shan J, Yuan C, Han W, Wan N, Chen Y. Synthesis of 2,3'-spirobi[indolin]-2-ones enabled by a tandem nucleophilic benzylation/C(sp2)-N cross-coupling reaction sequence. Org Biomol Chem. 2017;15:5887-5892 pubmed publisher
    ..This study represents a new perspective for the synthesis of structurally diverse spirocyclic oxindoles by employing 3-aminooxindole substrates. ..
  37. Neunstoecklin B, Villiger T, Lucas E, Stettler M, Broly H, Morbidelli M, et al. Pilot-scale verification of maximum tolerable hydrodynamic stress for mammalian cell culture. Appl Microbiol Biotechnol. 2016;100:3489-98 pubmed publisher
    ..A 300-L pilot-scale bioreactor for cultivation of a Sp2/0 cell line was used for this purpose...
  38. Goumon S, Å pinka M. Emotional contagion of distress in young pigs is potentiated by previous exposure to the same stressor. Anim Cogn. 2016;19:501-11 pubmed publisher
    ..Piglets (49.7 ± 0.7 days) were exposed in pairs to two stress phases (SP1 and SP2) in an arena divided into two pens by a wire mesh wall...
  39. Kaur B, Kaur R. Purification of a dimeric arginine deiminase from Enterococcus faecium GR7 and study of its anti-cancerous activity. Protein Expr Purif. 2016;125:53-60 pubmed publisher
    ..To assess anti-cancerous activity of ADI by MTT assay was carried out against cancer cell lines (MCF-7, Sp2/0-Ag14 and Hep-G2)...
  40. Marín Muñoz J, Noguera Perea M, Gomez Tortosa E, López Motos D, Antequera Torres M, Martínez Herrada B, et al. Novel Mutation (Gly212Val) in the PS2 Gene Associated with Early-Onset Familial Alzheimer's Disease. J Alzheimers Dis. 2016;53:73-8 pubmed publisher
    ..Lewy bodies were present but restricted to the amygdala. ..
  41. Cheng L, Wu H, Fei J, Zhang L, Ye J, Zhang H. [Preparation of monoclonal antibody against 4-amylphenol and homology modeling of its Fv fragment]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:390-396 pubmed
    ..generated from a hybridoma cell line F10 using electrofusion between splenocytes from APA-BSA-immunized mouse and Sp2/0 myeloma cells. Then we extracted the mRNA of F10 cells and cloned the cDNA of mAb...
  42. Liu J, Shi H, Chen J, Zhang X, Ji Z, Yuan J, et al. Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody. Virology. 2017;507:257-262 pubmed publisher
    ..After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained...
  43. Xu J, Liu Y, Zhang J, Xu X, Jin Z. Palladium-catalyzed enantioselective C(sp2)-H arylation of ferrocenyl ketones enabled by a chiral transient directing group. Chem Commun (Camb). 2018;54:689-692 pubmed publisher
    ..The transformation allows rapid access to ferrocene scaffolds simultaneously possessing planar- and stereogenic central chirality, widely applied in the ferrocene-based chiral ligand families. ..
  44. Li S, Xue F, Cheng Z, Yang X, Wang S, Geng F, et al. Effect of artesunate on inhibiting proliferation and inducing apoptosis of SP2/0 myeloma cells through affecting NFkappaB p65. Int J Hematol. 2009;90:513-521 pubmed publisher
    ..treatment of severe malaria, on inhibition of proliferation and induction of apoptosis of a mouse myeloma cell line SP2/0...
  45. Bian Z, Li X, Wu H, Xu J, Gu C, Chen X, et al. Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human Myeloperoxidase. Monoclon Antib Immunodiagn Immunother. 2016;35:86-93 pubmed publisher
    ..Splenocytes from these mice were fused with the mouse myeloma cell line SP2/0. Based on isotyping of the mAbs, both clones 1E3 and 3E8 were referred to the IgG1 subclass...
  46. Wu B, Melvina -, Wu X, Lee Yeow E, Yoshikai N. Versatile telluracycle synthesis via the sequential electrophilic telluration of C(sp2)-Zn and C(sp2)-H bonds. Chem Sci. 2017;8:4527-4532 pubmed publisher
    ..Furthermore, a variety of heteroarene-fused benzotellurophenes and other novel tellurium-embedded polycyclic aromatics can be readily synthesized from the corresponding 2-iodoheterobiaryls. ..
  47. Nathan S, Albera C, Bradford W, Costabel U, Glaspole I, Glassberg M, et al. Effect of pirfenidone on mortality: pooled analyses and meta-analyses of clinical trials in idiopathic pulmonary fibrosis. Lancet Respir Med. 2017;5:33-41 pubmed publisher
    ..did meta-analyses of these data and data from two Japanese trials of pirfenidone versus placebo-Shionogi Phase 2 (SP2) and Shionogi Phase 3 (SP3; trial durations 36-52 weeks)...
  48. Zhai S, Qiu S, Chen X, Wu J, Zhao H, Tao C, et al. 2-(1-Methylhydrazinyl)pyridine as a reductively removable directing group in a cobalt-catalyzed C(sp2)-H bond alkenylation/annulation cascade. Chem Commun (Camb). 2017;54:98-101 pubmed publisher
    ..The reaction shows a broad substrate scope with the products obtained in good to excellent yields and high regioselectivity. Moreover, the directing group can be reductively removed in one step under mild conditions. ..
  49. Sun J, Chen H, Davie J. Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. J Biol Chem. 2007;282:33227-36 pubmed
    ..We provide evidence that unmodified HDAC2 is associated with the coding region of transcribed genes, whereas phosphorylated HDAC2 is primarily recruited to promoters. ..
  50. Xiang J, Pi X, Chen X, Xiang L, Yang M, Ren H, et al. Integrated signal probe based aptasensor for dual-analyte detection. Biosens Bioelectron. 2017;96:268-274 pubmed publisher
    ..this work, two different aptamer probes labeled with redox tags were used as signal probe1 (sP1) and signal probe2 (sP2), which were integrated into one unity DNA architecture to develop the integrated signal probe (ISP)...
  51. Wang X, Xie P, Qiu R, Zhu L, Liu T, Li Y, et al. Nickel-catalysed direct alkylation of thiophenes via double C(sp3)-H/C(sp2)-H bond cleavage: the importance of KH2PO4. Chem Commun (Camb). 2017;53:8316-8319 pubmed publisher
    ..DFT calculations verify the importance of KH2PO4 as an additive for promoting C-H bond cleavage and support the involvement of a Ni(iii) species in the reaction. ..
  52. Hussain N, Tatina M, Rasool F, Mukherjee D. Diastereoselective sp2-sp3 coupling of sugar enol ethers with unactivated cycloalkenes: new entries to C-branched sugars. Org Biomol Chem. 2016;14:9989-9992 pubmed
    ..Generation of a ?-allyl complex by the Pd(ii) catalyst via cleavage of the allylic C-H bond of the cycloalkene may be responsible for the formation of sp2-sp3 coupling products. ..
  53. Ekren O, Ozkomur A, Ucar Y. Effect of layered manufacturing techniques, alloy powders, and layer thickness on metal-ceramic bond strength. J Prosthet Dent. 2018;119:481-487 pubmed publisher
    ..Statistical comparisons revealed that the highest bond strength could be achieved with DMLS-Cobalt-Chrome SP2-20μm, and the lowest bond strength was observed in DMLS-Keramit NP-S-20μm (P≤.05)...
  54. Yu W, Wen G, Lin H, Yang Y, Huang X, Zhou C, et al. Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802). Fish Shellfish Immunol. 2018;74:649-655 pubmed publisher
    ..The fish were fed for 8-week with diets containing different levels of S. platensis: 0% (C), 2% (SP2), 4% (SP4), 6% (SP6), 8% (SP8) and 10% (SP10) as treatment groups, followed by a Vibrio harveyi infection test for ..
  55. Yang T, Li Y, Ma M, Lin Q, Sun S, Zhang B, et al. Protective effect of soluble eggshell membrane protein hydrolysate on cardiac ischemia/reperfusion injury. Food Nutr Res. 2015;59:28870 pubmed publisher
    ..Rats and cardiomyocytes were pretreated with SP2, a hydrolysate attained from SEP, and then subjected to ischemia/reperfusion (I/R) or hypoxia/reoxygenation (H/R) ..
  56. Quan Y, Lyu H, Xie Z. Dehydrogenative cross-coupling of o-carborane with thiophenes via Ir-catalyzed regioselective cage B-H and C(sp2)-H activation. Chem Commun (Camb). 2017;53:4818-4821 pubmed publisher
  57. Li W, Gao G, Gao Y, Yang C, Xia W. Direct oxidation of the C(sp2)-C(sp3) bond from benzyltrimethylsilanes to phenols. Chem Commun (Camb). 2017;53:5291-5293 pubmed publisher
    ..The reaction shows good functional group tolerance to afford phenols in moderate yields. The possible mechanism is proposed based on the isotopic labeling trials. ..
  58. Liu Y, Zhao X, Guo C, Li Y, Wang G, Liang D, et al. [Preparation and identification of monoclonal antibody against H1N1 influenza virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:688-694 pubmed
    ..Hybridomas secreting mAbs were established through a fusion of Sp2/0 myeloma cells and splenocytes from the mice immunized with the virus...
  59. Tian G, Qi Z, Ma W, Wang Y. On the Catalytic Activity of Pt Supported by Graphyne in the Oxidation of Ethanol. ChemistrySelect. 2017;2:2311-2321 pubmed publisher
    ..Among the examined adsorption sites such as the hollow, Csp-Csp, and Csp-Csp2 bonds, the hollow site consisting of multiple triple bonds is the most attractive one to adsorb Pt and Pt..
  60. Uppunda A, Bhat J, D costa P, Raj M, Kumar K. Binaural Interaction Component in Speech Evoked Auditory Brainstem Responses. J Int Adv Otol. 2015;11:114-7 pubmed publisher
    ..The second BIC (BIC-SP2) was present around 8 ms in the latency region of peak A...
  61. Choi Y, Kim E, Han J, Kim J, Gurunathan S. A Novel Biomolecule-Mediated Reduction of Graphene Oxide: A Multifunctional Anti-Cancer Agent. Molecules. 2016;21:375 pubmed publisher
    ..In contrast, graphene is a two-dimensional sheet of sp2-hybridized carbon atoms packed into a honeycomb lattice...
  62. Suske G. NF-Y and SP transcription factors - New insights in a long-standing liaison. Biochim Biophys Acta Gene Regul Mech. 2017;1860:590-597 pubmed publisher
    ..coupled with high throughput sequencing (ChIP-seq) have examined binding of NF-Y and the ubiquitous SP factors SP1, SP2 and SP3 genome-wide, allowing for comprehensive comparison of NF-Y and SP factor actions in the context of ..
  63. NGUYEN T, Grigorjeva L, DAUGULIS O. Aminoquinoline-directed, cobalt-catalyzed carbonylation of sulfonamide sp2 C-H bonds. Chem Commun (Camb). 2017;53:5136-5138 pubmed publisher
    ..Halogen, ester, and amide functionalities are compatible with the reaction conditions. This method allows for a short and efficient synthesis of saccharin derivatives. ..
  64. Saint Maurice P, Kim Y, Welk G, Gaesser G. Kids are not little adults: what MET threshold captures sedentary behavior in children?. Eur J Appl Physiol. 2016;116:29-38 pubmed publisher
    ..0 adult-METs was used instead of 1.5 METs (Se1.5 METs = 4.7%, Sp1.5 METs = 100.0%; Se2.0 METs = 36.9%, Sp2.0 METs = 100.0 %). The use of child-METs while maintaining the 1...
  65. Schmidl S, Jakobs O, Guenther D, Lausmann C, Schoof B, Beckmann J, et al. Effective prevention of recurrent dislocation following primary cemented Endo-MarkIII/SP2 total hip arthroplasty using a posterior lip augmentation device. Arch Orthop Trauma Surg. 2016;136:579-83 pubmed publisher
    ..to determine the effectiveness of preventing recurrent dislocation following primary cemented Endo-MarkIII/SP2 total hip replacement using a posterior lip augmentation device (PLAD)...
  66. Li M, Berritt S, Matuszewski L, Deng G, Pascual Escudero A, Panetti G, et al. Transition-Metal-Free Radical C(sp3)-C(sp2) and C(sp3)-C(sp3) Coupling Enabled by 2-Azaallyls as Super-Electron-Donors and Coupling-Partners. J Am Chem Soc. 2017;139:16327-16333 pubmed publisher
    ..Radical clock studies and 2-azaallyl anion coupling studies provide mechanistic insight for this unique reactivity...
  67. Bakovic M, Waite K, Vance D. Functional significance of Sp1, Sp2, and Sp3 transcription factors in regulation of the murine CTP:phosphocholine cytidylyltransferase alpha promoter. J Lipid Res. 2000;41:583-94 pubmed
    ..expression of the CTalpha gene is controlled in part by the binding of three trans-acting nuclear factors, Sp1, Sp2, and Sp3...
  68. Rygus J, Crudden C. Enantiospecific and Iterative Suzuki-Miyaura Cross-Couplings. J Am Chem Soc. 2017;: pubmed publisher
    ..Though most widely utilized for the synthesis of sp2-sp2 linkages, the use of this reaction to form stereochemistry-bearing sp2-sp3 bonds has received widespread ..
  69. Philipsen S, Suske G. A tale of three fingers: the family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res. 1999;27:2991-3000 pubmed
    ..Currently this new family of transcription factors has at least 16 different mammalian members. Here, we will summarise and discuss recent advances that have been directed towards understanding the biological role of these proteins. ..
  70. Lee Y, Higashi Y, Luu C, Shimizu C, Strott C. Sp1 elements in SULT2B1b promoter and 5'-untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition. FEBS Lett. 2005;579:3639-45 pubmed
    ..Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly ..
  71. Rotheneder H, Geymayer S, Haidweger E. Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter. J Mol Biol. 1999;293:1005-15 pubmed
    ..murine fibroblast 3T6 cells demonstrate that the thymidine kinase promoter is activated strongly by Sp1 and Sp3, weakly by Sp4, and not at all by Sp2. Co-expression of E2F-1 results in synergistic activation in 3T6 but not in SL2 cells.
  72. Swamy V, Parvin N, Vipin Raj K, Vanka K, Sen S. C(sp3)-F, C(sp2)-F and C(sp3)-H bond activation at silicon(ii) centers. Chem Commun (Camb). 2017;53:9850-9853 pubmed publisher
    ..The scope of C-F bond activation has further been extended with C6F6 and C6F5CF3. ..
  73. Mishra A, Vats T, Nair M, Das A, Deb I. Rhodium-Catalyzed sp2 C-H Acetoxylation of N-Aryl Azaindoles/N-Heteroaryl Indolines. J Org Chem. 2017;: pubmed publisher
    ..The methodology developed could be successfully expanded for C7-acetoxylation of indoline derivatives using pyrimidine as a detachable directing group for the synthesis of 7-hydroxyindole...
  74. Cheng J, Shen L, Wu L, Hu X, Loh T. Iron-catalyzed peroxidation-carbamoylation of alkenes with hydroperoxides and formamides via formyl C(sp2)-H functionalization. Chem Commun (Camb). 2017;53:12830-12833 pubmed publisher
    ..The β-peroxy amide could serve as a synthetic precursor which was facilely converted to β-hydroxy amide, β-keto amide and β-lactam following subsequent chemical transformation. ..
  75. Kibriya G, Samanta S, Jana S, Mondal S, Hajra A. Visible-Light Organic Photoredox-Catalyzed C-H Alkoxylation of Imidazopyridine with Alcohol. J Org Chem. 2017;: pubmed publisher
    ..A wide range of functional groups could be tolerated under the reaction condition to produce C(sp2)-H alkoxylated products in high yields.
  76. Tsukano C, Suetsugu S, Muto N, Takemoto Y. Synthesis of Tetrahydrobiphenylene via Pd(0)-Catalyzed C(sp2)-H Functionalization. Chem Pharm Bull (Tokyo). 2017;65:1167-1174 pubmed publisher
    ..The developed method was applied to the synthesis of several tetrahydrobiphenylenes having an oxygen functionality at the ring juncture. The derivatization of a tetrahydrobiphenylene is also reported...
  77. Pahl A, Waldmann H, Kumar K. Exploring Natural Product Fragments for Drug and Probe Discovery. Chimia (Aarau). 2017;71:653-660 pubmed publisher
    ..The majority of the fragments employed so far is aromatic and sp2-configured, and there is a high demand of fragments with stereogenic centers...
  78. Wilson C, Ganesh V, Noble A, Aggarwal V. Enantiospecific sp²-sp³ Coupling of o- and p-Phenols with Secondary and Tertiary Boronic Esters. Angew Chem Int Ed Engl. 2017;: pubmed publisher
    ..For o-substituted phenols, initial incorporation of a benzotriazole on the phenol oxygen was required. Subsequent ortholithiation and borylation gave the coupled product, again with complete stereospecificity...
  79. Kuang Z, Li B, Song Q. Cu/Pd cooperatively catalyzed tandem intramolecular anti-Markovnikov hydroarylation of unsaturated amides: facile construction of 3,4-dihydroquinolinones via borylation/intramolecular C(sp3)-C(sp2) cross coupling. Chem Commun (Camb). 2017;54:34-37 pubmed publisher
    ..3,4-Dihydroquinolinones, one type of important scaffold prevailing in pharmaceuticals and biologically active compounds, could be readily accessible with high regio-selectivity and high yields. ..
  80. Zhang S, Li L, Wang H, Li Q, Liu W, Xu K, et al. Scalable Electrochemical Dehydrogenative Lactonization of C(sp2/sp3)-H Bonds. Org Lett. 2017;: pubmed publisher
    ..The remote lactonization of C(sp3)-H bonds would constitute an important synthetic advance toward electrochemical C-O bond formation...
  81. Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol Cell Biol. 1992;12:4251-61 pubmed
    ..Two of them, which we term Sp2 and Sp3, were cloned. Sp2 and Sp3 contain zinc fingers and transactivation domains similar to those of Sp1...
  82. Abdiaj I, Alcazar J. Improving the throughput of batch photochemical reactions using flow: Dual photoredox and nickel catalysis in flow for C(sp2)C(sp3) cross-coupling. Bioorg Med Chem. 2017;25:6190-6196 pubmed publisher
    ..This new procedure clearly improves the scalability of the previous batch reaction by the reactor's size and operating time reduction, and allows the preparation of interesting compounds for drug discovery in multigram amounts. ..
  83. Hurej K, Pawlicki M, Latos Grazynski L. Rhodium-induced reversible C-C bond cleavage - transformations of rhodium(III) 22-alkyl-m-benziporphyrins. Chemistry. 2017;: pubmed publisher
    ..triggers the ethyl migration from carbon(22) to rhodium(III) affording the diamagnetic rhodium(III) meta-benziporphyrin containing the apically coordinated ?-ethyl ligand providing an example of reversible C(sp2)-C(sp3) bond cleavage.
  84. Moorefield K, Fry S, Horowitz J. Sp2 DNA binding activity and trans-activation are negatively regulated in mammalian cells. J Biol Chem. 2004;279:13911-24 pubmed
    Previous studies have indicated that Sp2 binds poorly to GC-rich sequences bound by Sp1 and Sp3, and further functional analyses of Sp2 have been limited...
  85. Wang R, Li Y, Jin R, Wang X. Copper-catalyzed oxidative C(sp3)-H/C(sp2)-H cross-coupling en route to carbocyclic rings. Chem Sci. 2017;8:3838-3842 pubmed publisher
    ..An aniline module was used as a directing group to generate an aminyl radical, which selectively cleaves the secondary and tertiary C(sp3)-H bonds via a 1,5-HAT process to forge six-membered carbocyclic rings. ..
  86. Sattar M, Kumar S. Palladium-Catalyzed Removable 8-Aminoquinoline Assisted Chemo- and Regioselective Oxidative sp2-C-H/sp3-C-H Cross-Coupling of Ferrocene with Toluene Derivatives. Org Lett. 2017;19:5960-5963 pubmed publisher
    ..It seems Fe(II) catalyst not only stabilizes the generated benzyl radical but also tames its oxidizing behavior, and consequently transfers it to the palladacycle for C-C coupling...
  87. Kleinerman O, Adnan M, Marincel D, Ma A, Bengio E, Park C, et al. Dissolution and Characterization of Boron Nitride Nanotubes in Superacid. Langmuir. 2017;: pubmed publisher
    ..Electron energy loss spectroscopy of BNNTs dried from the solution confirms preservation of the sp2 hybridization for boron and nitrogen, eliminating the possibility of BNNT functionalization or damage...
  88. Alcalde Estévez E, Arroba A, Sánchez Fernández E, Mellet C, García Fernández J, Masgrau L, et al. The sp2-iminosugar glycolipid 1-dodecylsulfonyl-5N,6O-oxomethylidenenojirimycin (DSO2-ONJ) as selective anti-inflammatory agent by modulation of hemeoxygenase-1 in Bv.2 microglial cells and retinal explants. Food Chem Toxicol. 2018;111:454-466 pubmed publisher
    ..Since IL-10-mediated induction of HO-1 expression is mediated by p38α MAPK activation, our results suggest that this molecular mechanism is involved in the anti-inflammatory effects of DSO2-ONJ in microglia. ..
  89. Yang L, Neuburger M, Baudoin O. Introducing Chiral Bifunctional Phosphine-Carboxylate Ligands for Palladium(0)-Catalyzed Enantioselective C-H Arylation. Angew Chem Int Ed Engl. 2017;: pubmed publisher
    ..The optimal ligand provided high yields and enantioselectivities for the desymmetrizing C(sp2)-H arylation leading to 5,6-dihydrophenanthridines, whereas the corresponding monofunctional ligands showed low ..
  90. Phan D, Cheng C, Galfione M, Vakar Lopez F, Tunstead J, Thompson N, et al. Identification of Sp2 as a transcriptional repressor of carcinoembryonic antigen-related cell adhesion molecule 1 in tumorigenesis. Cancer Res. 2004;64:3072-8 pubmed
    ..of CEACAM1 expression in prostate cancer cells occurs primarily at the transcriptional level and is mediated by Sp2, a member of the Sp family of transcription factors...
  91. Moorefield K, Yin H, Nichols T, Cathcart C, Simmons S, Horowitz J. Sp2 localizes to subnuclear foci associated with the nuclear matrix. Mol Biol Cell. 2006;17:1711-22 pubmed
    We have reported that extracts prepared from many human and mouse cell lines show little or no Sp2 DNA-binding activity and that Sp2 has little or no capacity to stimulate transcription of promoters that are activated by Sp1, Sp3, and ..
  92. Ito H, Murakami M, Furuhata A, Gao S, Yoshida K, Sobue S, et al. Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin. Biochim Biophys Acta. 2009;1789:681-90 pubmed publisher
    ..In SL2 cells lacking Sp family proteins, both Sp1 and Sp3 overexpression increased NSMase promoter activity. Increased binding of Sp family proteins by DA to three Sp1 motifs was shown by electrophoresis mobility shift and ChIP assays. ..