SMAD5

Summary

Gene Symbol: SMAD5
Description: SMAD family member 5
Alias: DWFC, JV5-1, MADH5, mothers against decapentaplegic homolog 5, MAD, mothers against decapentaplegic homolog 5, SMA- and MAD-related protein 5, SMAD, mothers against DPP homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5
Species: human
Products:     SMAD5

Top Publications

  1. Massague J. TGF-beta signal transduction. Annu Rev Biochem. 1998;67:753-91 pubmed
    ..Mutations in these pathways are the cause of various forms of human cancer and developmental disorders. ..
  2. Hanai J, Chen L, Kanno T, Ohtani Fujita N, Kim W, Guo W, et al. Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic induction of the immunoglobulin germline Calpha promoter. J Biol Chem. 1999;274:31577-82 pubmed
    ..PEBP2 may thus be a nuclear target of TGF-beta/BMP signaling. ..
  3. Zaidi S, Sullivan A, Van Wijnen A, Stein J, Stein G, Lian J. Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites. Proc Natl Acad Sci U S A. 2002;99:8048-53 pubmed
    ..Thus, Runx-mediated intranuclear targeting of Smads is critical for the integration of two distinct pathways essential for fetal development. ..
  4. Chen D, Zhao M, Mundy G. Bone morphogenetic proteins. Growth Factors. 2004;22:233-41 pubmed
    ..Natl Acad. Sci. USA 94, 12938-12943; Nishimura R. et al. (1998) "Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal ..
  5. Lu K, Yin X, Weng T, Xi S, Li L, Xing G, et al. Targeting WW domains linker of HECT-type ubiquitin ligase Smurf1 for activation by CKIP-1. Nat Cell Biol. 2008;10:994-1002 pubmed publisher
    ..These findings provide evidence that the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and that CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. ..
  6. Nie J, Xie P, Liu L, Xing G, Chang Z, Yin Y, et al. Smad ubiquitylation regulatory factor 1/2 (Smurf1/2) promotes p53 degradation by stabilizing the E3 ligase MDM2. J Biol Chem. 2010;285:22818-30 pubmed publisher
    ..To our knowledge, this is the first report to demonstrate that Smurf1/2 functions as a factor to stabilize MDM2 protein rather than as a direct E3 ligase in regulation of p53 degradation. ..
  7. Nohe A, Keating E, Knaus P, Petersen N. Signal transduction of bone morphogenetic protein receptors. Cell Signal. 2004;16:291-9 pubmed
    ..This article reviews the current state of BMP receptor signaling and provides a summary of the crosstalk of the BMP receptor pathway with other major signaling pathways. ..
  8. Chang S, Chang C, Lee D, Lee P, Yeh Y, Yeh C, et al. Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad. Proc Natl Acad Sci U S A. 2008;105:3927-32 pubmed publisher
    ..by alpha(v)beta(3) and beta(1) integrins through bone morphogenetic protein receptor type IA-specific Smad1 and Smad5. Shear stress also down-regulated runt-related transcription factor 2 (Runx2) binding activity and osteocalcin and ..
  9. Middlebrook B, Eldin K, Li X, Shivasankaran S, Pangas S. Smad1-Smad5 ovarian conditional knockout mice develop a disease profile similar to the juvenile form of human granulosa cell tumors. Endocrinology. 2009;150:5208-17 pubmed publisher
    ..The TGFbeta family is active in mouse Smad1-Smad5 double knockout tumors, and here we show that this pathway, but not the beta-catenin pathway, is activated in ..

More Information

Publications190 found, 100 shown here

  1. Liu C, Chen L, Zeng J, Cui J, Ning J, Wang G, et al. Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling. Exp Cell Res. 2015;336:109-18 pubmed publisher
    ..role of BMP2 in the CBDL-rat serum-induced myogenic differentiation of PMVECs via the activation of both Smad1 and Smad5 and the down-regulation of Smurf1, which may represent a potential therapy for HPS-induced pulmonary vascular ..
  2. Celić T, Omrčen H, Spanjol J, Bobinac D. Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats. Transplant Proc. 2018;50:3822-3830 pubmed publisher
    ..Also, in kidneys perfused with rhBMP-7 solution, statistically higher levels of Smad1, Smad5, and Smad8 messenger RNA expressions were proven...
  3. Fu Q, Yang F, Zhao J, Yang X, Xiang T, Huai G, et al. Bioinformatical identification of key pathways and genes in human hepatocellular carcinoma after CSN5 depletion. Cell Signal. 2018;49:79-86 pubmed publisher
    ..encyclopedia of DNA elements (ENCODE) analysis showed that CSN5 depletion took effects through down-regulation of SMAD5-related pathways which include EXO1, CENPA and NCAPG, resulting in the inactivation of H3K4me3 and H3K36me3...
  4. Ishikawa K, Sreekumar P, Spee C, Nazari H, Zhu D, Kannan R, et al. αB-Crystallin Regulates Subretinal Fibrosis by Modulation of Epithelial-Mesenchymal Transition. Am J Pathol. 2016;186:859-73 pubmed publisher
    ..Overexpression of αB-crystallin enhanced nuclear translocation and accumulation of SMAD4 and SMAD5. Thus, αB-crystallin is an important regulator of EMT, acting as a molecular chaperone for SMAD4 and as its ..
  5. Helbing T, Wiltgen G, Hornstein A, Brauers E, Arnold L, Bauer A, et al. Bone Morphogenetic Protein-Modulator BMPER Regulates Endothelial Barrier Function. Inflammation. 2017;40:442-453 pubmed publisher
    ..High levels of BMPER antagonized BMP4-Smad5-Id1 signaling and prevented BMP4-induced downregulation of VE-cadherin and endothelial leakage, suggesting that ..
  6. Liu T, Zou X, Huang N, Ge X, Yao M, Liu H, et al. miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Life Sci. 2019;: pubmed publisher
    ..Furthermore, elevated expression of miR-27a suppressed bone morphogenetic protein (BMP) signaling by targeting Smad5, thereby lessening Id2-mediated repression of the 2 critical mediators of EndMT (Snail and Twist)...
  7. Yang C, Shao T, Zhang H, Zhang N, Shi X, Liu X, et al. MiR-425 expression profiling in acute myeloid leukemia might guide the treatment choice between allogeneic transplantation and chemotherapy. J Transl Med. 2018;16:267 pubmed publisher
    ..Among these genes, MAP3K5, SMAD2, and SMAD5 were predicted targets of miR-425...
  8. Gámez B, Rodríguez Carballo E, Graupera M, Rosa J, Ventura F. Class I PI-3-Kinase Signaling Is Critical for Bone Formation Through Regulation of SMAD1 Activity in Osteoblasts. J Bone Miner Res. 2016;31:1617-30 pubmed publisher
    ..Accordingly, pharmacological inhibition of GSK3 activity or ectopic expression of SMAD1 or SMAD5 normalizes bone morphogenetic protein (BMP) transduction and osteoblast differentiation...
  9. Hadjimichael C, Nikolaou C, Papamatheakis J, Kretsovali A. MicroRNAs for Fine-Tuning of Mouse Embryonic Stem Cell Fate Decision through Regulation of TGF-β Signaling. Stem Cell Reports. 2016;6:292-301 pubmed publisher
    ..of the core pluripotency transcription factor Oct4 and the bone morphogenetic protein (BMP)-signaling components, Smad5 and Id2...
  10. Zhu G, Fang C, Li J, Mo C, Wang Y, Li J. Transcriptomic Diversification of Granulosa Cells during Follicular Development in Chicken. Sci Rep. 2019;9:5462 pubmed publisher
    ..of many genes characterized in cell signaling (AMH, Inhibins, Activins, BMPs) and transcription factors (SMAD3, SMAD5, ID1, ID2, ID3)...
  11. Zhang X, Ai F, Li X, She X, Li N, Tang A, et al. Inflammation-induced S100A8 activates Id3 and promotes colorectal tumorigenesis. Int J Cancer. 2015;137:2803-14 pubmed publisher
    ..Id3 expression was regulated by Smad5, which was directly phosphorylated by Akt1...
  12. Xie K, Ma H, Liang C, Wang C, Qin N, Shen W, et al. A functional variant in miR-155 regulation region contributes to lung cancer risk and survival. Oncotarget. 2015;6:42781-92 pubmed publisher
    ..activity of miR-155, which in turn facilitated tumor growth and metastasis by inhibiting HBP1, TJP1, SMAD5 and PRKAR1A expression...
  13. Swartz M, Sheehan Rooney K, Dixon M, Eberhart J. Examination of a palatogenic gene program in zebrafish. Dev Dyn. 2011;240:2204-20 pubmed publisher
    ..Functional investigation of a subset of these genes, fgf10a, tgfb2, pax9, and smad5 revealed their necessity in zebrafish palatogenesis...
  14. Lao C, Zhang Y, Li W, Li M, Yang H. [Preliminary study on the biological effects of MiR-144 in pulmonary injury in rats induced by nanosized SiOâ‚‚]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33:641-5 pubmed
    ..They are SMAD4, SMAD5, ADAMTS3, ADAMTS15 and ADAMTS19...
  15. Grünhagen J, Bhushan R, Degenkolbe E, Jäger M, Knaus P, Mundlos S, et al. MiR-497∼195 cluster microRNAs regulate osteoblast differentiation by targeting BMP signaling. J Bone Miner Res. 2015;30:796-808 pubmed publisher
    ..pro-forms, genes encoding receptors such as Acvr2a, Bmp1a, Dies1, and Tgfbr3, molecules within the cascade like Smad5, transcriptional regulators like Ski and Zfp423 as well as Mapk3 and Smurf1 were validated by quantitative PCR...
  16. Cafri G, Yossef R, Pasetto A, Deniger D, Lu Y, Parkhurst M, et al. Memory T cells targeting oncogenic mutations detected in peripheral blood of epithelial cancer patients. Nat Commun. 2019;10:449 pubmed publisher
    ..two metastatic colon cancer patients, we detected CD8+ neoantigen-specific cells targeting the mutated SMAD5 and MUC4 proteins...
  17. Souza T, van den Beucken T, Kleinjans J, Jennen D. Inferring transcription factor activity from microarray data reveals novel targets for toxicological investigations. Toxicology. 2017;389:101-107 pubmed publisher
    ..SMADs (SMAD1, SMAD2, SMAD5) and KLF5 were identified as some of potentially new TFs whose inferred activities were linked to acute and ..
  18. Alkema M, Goumans M, Kruithof B. Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections. Methods Mol Biol. 2019;1891:191-200 pubmed publisher
    ..Phosphorylated Smad1, Smad5, and Smad9 are the activated signaling molecules of the BMP pathway that transfer BMP signals from the cell ..
  19. Curado F, Spuul P, Egaña I, Rottiers P, Daubon T, Veillat V, et al. ALK5 and ALK1 play antagonistic roles in transforming growth factor β-induced podosome formation in aortic endothelial cells. Mol Cell Biol. 2014;34:4389-403 pubmed publisher
    ..induced by BMP9 also antagonizes TGF-β-induced podosome formation, but this occurs through both Smad1 and Smad5. Whereas ALK1 neutralization brings ALK5 signals to full potency for TGF-β-induced podosome formation, ALK1 ..
  20. Mansouri Attia N, Tripurani S, Gokul N, Piard H, Anderson M, Eldin K, et al. TGFβ signaling promotes juvenile granulosa cell tumorigenesis by suppressing apoptosis. Mol Endocrinol. 2014;28:1887-98 pubmed publisher
    ..deletion in granulosa cells of the bone morphogenetic protein receptor-signaling transcription factors, Smad1 and Smad5, causes development of metastatic granulosa cell tumors that phenocopy the juvenile form of granulosa cell tumors (..
  21. Watanabe Y, Papoutsoglou P, Maturi V, Tsubakihara Y, Hottiger M, Heldin C, et al. Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation. J Biol Chem. 2016;291:12706-23 pubmed publisher
    ..PARG positively contributes to BMP signaling and forms physical complexes with Smad5 and Smad4...
  22. Wang H, Jiang Y, Lu M, Sun B, Qiao X, Xue D, et al. STX12 lncRNA/miR-148a/SMAD5 participate in the regulation of pancreatic stellate cell activation through a mechanism involving competing endogenous RNA. Pancreatology. 2017;17:237-246 pubmed publisher
    ..The expression of the interleukin 6 signal transducer (IL6ST), SMAD family member 5 (SMAD5) and alpha smooth muscle actin (?-SMA) proteins were examined by western blot...
  23. Sasaki A, Masuda Y, Ohta Y, Ikeda K, Watanabe K. Filamin associates with Smads and regulates transforming growth factor-beta signaling. J Biol Chem. 2001;276:17871-7 pubmed
    ..We performed yeast two-hybrid screening and identified filamin, a cytoskeletal actin-binding protein 280, as a Smad5-interacting protein...
  24. Xing C, Gong B, Xue Y, Han Y, Wang Y, Meng A, et al. TGFβ1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway. J Mol Cell Biol. 2015;7:48-61 pubmed publisher
    ..Like tgfβ1a depletion, knockdown of smad5 that expresses in the pLLP, affects pLLP development whereas overexpression of a constitutive active Smad5 isoform ..
  25. Marks Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, et al. SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol Cell Biol. 2015;35:2165-72 pubmed publisher
    ..SMAD1 and SMAD5, the core mediators of BMP signaling, are vital for this activity, yet little is known about their transcriptional ..
  26. Lulli V, Buccarelli M, Martini M, Signore M, Biffoni M, Giannetti S, et al. miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal. Oncotarget. 2015;6:37241-56 pubmed publisher
    ..We identified ADAM12 and confirmed SMAD5 and GSK3β as miR-135b targets and potential mediators of its effects...
  27. George J, Lewis M, Renne R, Mattapallil J. Suppression of transforming growth factor β receptor 2 and Smad5 is associated with high levels of microRNA miR-155 in the oral mucosa during chronic simian immunodeficiency virus infection. J Virol. 2015;89:2972-8 pubmed publisher
    ..mucosa during chronic SIV infection and was coincident with downregulation of TGFβ receptor 2 (TGFβ-R2) and SMAD5, key TGFβ signaling genes that harbor putative target sites for miR-155...
  28. Miyazono K. TGF-beta signaling by Smad proteins. Cytokine Growth Factor Rev. 2000;11:15-22 pubmed
    ..Through interaction with different transcription factors and transcriptional co-activators or co-repressors, Smads may exhibit specific effects in various cell types. ..
  29. Davis B, Hilyard A, Lagna G, Hata A. SMAD proteins control DROSHA-mediated microRNA maturation. Nature. 2008;454:56-61 pubmed publisher
  30. Quillien A, Blanco Sanchez B, Halluin C, Moore J, Lawson N, Blader P, et al. BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development. 2011;138:2293-302 pubmed publisher
    ..indicate that both the induction of a photoreceptor fate and the interaction with Notch relies on a canonical BMP/Smad5 pathway...
  31. Kaneko K, Higuchi C, Kunugiza Y, Yoshida K, Sakai T, Yoshikawa H, et al. Hyaluronan inhibits BMP-induced osteoblast differentiation. FEBS Lett. 2015;589:447-54 pubmed publisher
    ..BMP-induced osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation were downregulated by HA...
  32. Tao S, Sampath K. Alternative splicing of SMADs in differentiation and tissue homeostasis. Dev Growth Differ. 2010;52:335-42 pubmed publisher
    ..Here, we discuss the roles of various SMAD isoforms, and the consequences of mis-regulation of SMAD splicing in development and tissue homeostasis. ..
  33. Grider A, Lewis R, Laing E, Bakre A, Tripp R. Zinc affects miR-548n, SMAD4, SMAD5 expression in HepG2 hepatocyte and HEp-2 lung cell lines. Biometals. 2015;28:959-66 pubmed publisher
    ..study was to determine the effects of Zn concentration in cell culture on the expression of miR-548n, SMAD4 and SMAD5 in hepatocyte (HepG2) and lung epithelium (HEp-2) cell lines...
  34. Wang M, Sun J, Xu B, Chruściel M, Gao J, Bazert M, et al. Functional Characterization of MicroRNA-27a-3p Expression in Human Polycystic Ovary Syndrome. Endocrinology. 2018;159:297-309 pubmed publisher
    ..The overexpression of miR-27a-3p in KGN cells inhibited SMAD5, which in turn decreased cell proliferation and promoted cell apoptosis...
  35. Nishida T, Kubota S, Aoyama E, Takigawa M. Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus. Osteoarthritis Cartilage. 2013;21:700-9 pubmed publisher
    ..These findings suggest that decreased ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad1/5/8 in the presence of lactate. Novel metabolic aspects of OA pathogenesis are indicated herein. ..
  36. Latour C, Besson Fournier C, Meynard D, Silvestri L, Gourbeyre O, Aguilar Martinez P, et al. Differing impact of the deletion of hemochromatosis-associated molecules HFE and transferrin receptor-2 on the iron phenotype of mice lacking bone morphogenetic protein 6 or hemojuvelin. Hepatology. 2016;63:126-37 pubmed publisher
    ..was not affected by loss of Hfe or Tfr2, that of Bmp6-deficient females was considerably worsened, with decreased Smad5 phosphorylation, compared with single Bmp6-deficient mice, further repression of hepcidin gene expression, ..
  37. Yoon W, Islam R, Cho Y, Ryu K, Shin H, Woo K, et al. Pin1 plays a critical role as a molecular switch in canonical BMP signaling. J Cell Physiol. 2015;230:640-7 pubmed publisher
    ..in the bone matrix plays a critical role in adult bone maintenance, we suspected that BMP R-Smads (Smad1 and Smad5) could be critical targets for Pin1 action...
  38. Tsukamoto S, Mizuta T, Fujimoto M, Ohte S, Osawa K, Miyamoto A, et al. Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling. Sci Rep. 2014;4:7596 pubmed publisher
    Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation by bone morphogenetic protein (BMP)-bound type I receptor kinases...
  39. Li G, Liu X, Li X, Gao Z, Huang L, Liu Y. [Regulations of berberine on gene expression of BMP4 transcriptional pathways to improve visceral white adipose tissues insulin resistance in type 2 diabetic hamsters]. Zhongguo Zhong Yao Za Zhi. 2016;41:514-520 pubmed publisher
    ..The results showed that the gene mRNA expressions of BMP4, BMPR?, BMPRlA, Smad1, Smad5, Smad8, p38/MAPK, ATF2, PRDM16, C/EBP?, PGC1?, PPAR? and brown adipose tissue-specific genes was decreased and ..
  40. Frontelo P, Leader J, Yoo N, Potocki A, Crawford M, Kulik M, et al. Suv39h histone methyltransferases interact with Smads and cooperate in BMP-induced repression. Oncogene. 2004;23:5242-51 pubmed
    ..to identify novel partners of Smad proteins in transcriptional regulation, we performed a two-hybrid screen using Smad5, a protein that is activated predominantly by bone morphogenetic protein (BMP) signaling...
  41. Yan J, Guo D, Yang S, Sun H, Wu B, Zhou D. Inhibition of miR-222-3p activity promoted osteogenic differentiation of hBMSCs by regulating Smad5-RUNX2 signal axis. Biochem Biophys Res Commun. 2016;470:498-503 pubmed publisher
    ..Whereas, overexpression of miR-222-3p inhibited osteoblast differentiation of hBMSCs in vitro. Moreover, Smad5 and RUNX2, which are the critical transcription factors in osteogenic differentiation, were predicted to be ..
  42. Gregoire J, Fleury L, Salazar Cardozo C, Alby F, Masson V, Arimondo P, et al. Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells. BMC Cancer. 2016;16:700 pubmed publisher
    ..70 gene candidates among which some are described to be, directly or indirectly, involved in EMT like ZEB1, G9a, SMAD5 and SMARCD3. We also identified the DOT1L as involved in EMT regulation in MDA-MB-231...
  43. Du C, Pan P, Jiang Y, Zhang Q, Bao J, Liu C. Microarray data analysis to identify crucial genes regulated by CEBPB in human SNB19 glioma cells. World J Surg Oncol. 2016;14:258 pubmed
    ..CCL2), focal adhesion (which involved THBS1 and THBS2), TGF-beta signaling pathway (which involved THBS1, THBS2, SMAD5, and SMAD6) and chronic myeloid leukemia (which involved TGFBR2 and CCND1)...
  44. Huang K, Bao H, Yan Z, Wang L, Zhang P, Yao Q, et al. MicroRNA-33 protects against neointimal hyperplasia induced by arterial mechanical stretch in the grafted vein. Cardiovasc Res. 2017;113:488-497 pubmed publisher
    ..of bone morphogenetic protein 3 (BMP3), which is a putative target of miR-33, and the phosphorylation of smad2 and smad5, which are potential downstream targets of BMP3, were increased in the grafted veins...
  45. Moya I, Umans L, Maas E, Pereira P, Beets K, Francis A, et al. Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 2012;22:501-14 pubmed publisher
    ..We report here that Smad1/Smad5-mediated BMP signaling synergizes with Notch signaling during selection of tip and stalk cells...
  46. Iezaki T, Onishi Y, Ozaki K, Fukasawa K, Takahata Y, Nakamura Y, et al. The Transcriptional Modulator Interferon-Related Developmental Regulator 1 in Osteoblasts Suppresses Bone Formation and Promotes Bone Resorption. J Bone Miner Res. 2016;31:573-84 pubmed publisher
    ..export of p65 and a decrease in NF-κB-dependent Smad7 expression and the subsequent enhancement of Smad1/Smad5/Smad8-dependent transcription...
  47. Rodriguez A, Tripurani S, Burton J, Clementi C, Larina I, Pangas S. SMAD Signaling Is Required for Structural Integrity of the Female Reproductive Tract and Uterine Function During Early Pregnancy in Mice. Biol Reprod. 2016;95:44 pubmed publisher
    ..Here, we used a mouse model containing triple conditional deletion of the BMP receptor signaling Smads (Smad1 and Smad5) and Smad4, the central mediator of both TGFbeta and BMP signaling, to investigate the role of the SMADs in ..
  48. Shintani M, Yagi H, Nakayama T, Saji T, Matsuoka R. A new nonsense mutation of SMAD8 associated with pulmonary arterial hypertension. J Med Genet. 2009;46:331-7 pubmed publisher
    ..We screened for mutations in ENDOGLIN(ENG), SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6 and SMAD8 genes, which are involved in the TGF-beta/BMP signallings, in 23 patients with IPAH who had no ..
  49. Lu K, Li P, Zhang M, Xing G, Li X, Zhou W, et al. Pivotal role of the C2 domain of the Smurf1 ubiquitin ligase in substrate selection. J Biol Chem. 2011;286:16861-70 pubmed publisher
    ..a key role in localization to the plasma membrane and endows Smurf1 with differential activity toward RhoA versus Smad5 and Runx2...
  50. Perdigão Henriques R, Petrocca F, Altschuler G, Thomas M, Le M, Tan S, et al. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Oncogene. 2016;35:158-72 pubmed publisher
    ..Twelve targets of miR-200c (Crtap, Fhod1, Smad2, Map3k1, Tob1, Ywhag/14-3-3γ, Ywhab/14-3-3β, Smad5, Zfp36, Xbp1, Mapk12, Snail1) were experimentally validated by identifying their 3'UTR miR-200 recognition ..
  51. Macias Silva M, Hoodless P, Tang S, Buchwald M, Wrana J. Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. J Biol Chem. 1998;273:25628-36 pubmed
    ..active ALK2 associated with and phosphorylated Smad1 on the COOH-terminal SSXS motif, and also regulated Smad5 and Smad8 phosphorylation. Activated ALK2 also induced the Tlx2 promoter in the absence of BMP7...
  52. Miyoshi T, Otsuka F, Suzuki J, Takeda M, Inagaki K, Kano Y, et al. Mutual regulation of follicle-stimulating hormone signaling and bone morphogenetic protein system in human granulosa cells. Biol Reprod. 2006;74:1073-82 pubmed
    ..FSH also augmented SMAD1 and SMAD5 expression, and conversely, FSH suppressed the expression of the inhibitory SMADs, SMAD6 and SMAD7...
  53. Hammond K, Loynes H, Mowbray C, Runke G, Hammerschmidt M, Mullins M, et al. A late role for bmp2b in the morphogenesis of semicircular canal ducts in the zebrafish inner ear. PLoS ONE. 2009;4:e4368 pubmed publisher
    ..Injection of bmp2b or smad5 mRNA into homozygous mutant swirl (bmp2b(-/-)) embryos rescues the early patterning defects in these mutants and ..
  54. Thériault B, Nachtigal M. Human ovarian cancer cell morphology, motility, and proliferation are differentially influenced by autocrine TGF? superfamily signalling. Cancer Lett. 2011;313:108-21 pubmed publisher
    ..These results suggest that a balance between BMP and TGF?/activin signalling may be altered to favour BMP signalling during ovarian cancer metastatic progression. ..
  55. Araújo V, Silva G, Duarte A, Magalhães Padilha D, Almeida A, Lunardi F, et al. Bone Morphogenetic Protein-6 (BMP-6) Stimulates the Antrum Formation by the Regulation of its Signalling Pathway in Caprine Pre-antral Follicles Cultured In Vitro. Reprod Domest Anim. 2016;51:59-68 pubmed publisher
    ..Experiment 1) and the mRNA levels for BMP receptors/Smad signalling pathway (BMPR1A, BMPR2, SMAD1, SMAD4, SMAD5, SMAD6, SMAD7 and SMAD8) in vivo and in vitro using BMP-6 (Experiment 2)...
  56. Guo Y, Hai Y, Yao C, Chen Z, Hou J, Li Z, et al. Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1/5 activation. Cell Commun Signal. 2015;13:20 pubmed publisher
    ..This study could provide adequate human Sertoli cells for reproductive and regenerative medicine. ..
  57. Takeda M, Mizuide M, Oka M, Watabe T, Inoue H, Suzuki H, et al. Interaction with Smad4 is indispensable for suppression of BMP signaling by c-Ski. Mol Biol Cell. 2004;15:963-72 pubmed
    ..a transcriptional corepressor that interacts strongly with Smad2, Smad3, and Smad4 but only weakly with Smad1 and Smad5. Through binding to Smad proteins, c-Ski suppresses signaling of transforming growth factor-beta (TGF-beta) as ..
  58. Liu Y, Ren W, Warburton R, Toksoz D, Fanburg B. Serotonin induces Rho/ROCK-dependent activation of Smads 1/5/8 in pulmonary artery smooth muscle cells. FASEB J. 2009;23:2299-306 pubmed publisher
    ..Rho and Rho kinase also participate in the activation of Smads by BMP. ..
  59. Nurgazieva D, Mickley A, Moganti K, Ming W, Ovsyi I, Popova A, et al. TGF-β1, but not bone morphogenetic proteins, activates Smad1/5 pathway in primary human macrophages and induces expression of proatherogenic genes. J Immunol. 2015;194:709-18 pubmed publisher
  60. Ono Y, Calhabeu F, Morgan J, Katagiri T, Amthor H, Zammit P. BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells. Cell Death Differ. 2011;18:222-34 pubmed publisher
    ..of BMP signalling by siRNA-mediated knockdown of BMPR-1A, disruption of the intracellular pathway by either Smad5 or Smad4 knockdown or inhibition of Smad1/5/8 phosphorylation with Dorsomorphin, also caused premature myogenic ..
  61. Huo L, Liu K, Pei J, Yang Y, Ye Y, Liu Y, et al. Fluoride promotes viability and differentiation of osteoblast-like Saos-2 cells via BMP/Smads signaling pathway. Biol Trace Elem Res. 2013;155:142-9 pubmed publisher
    ..The present results reveal that BMP/Smad signaling pathway was altered during the period of osteogenesis, and that the activities of p-Smad1/5 were required for Saos-2 cells viability and differentiation induced by fluoride. ..
  62. Miyasaka M, Nakata H, Hao J, Kim Y, Kasugai S, Kuroda S. Low-Intensity Pulsed Ultrasound Stimulation Enhances Heat-Shock Protein 90 and Mineralized Nodule Formation in Mouse Calvaria-Derived Osteoblasts. Tissue Eng Part A. 2015;21:2829-39 pubmed publisher
    ..LIPUS as well as heat shock initially upregulated HSP90 and phosphorylation of Smad1 and Smad5, encouraging cell viability and proliferation at 24 h, enhancing mineralized nodule formation stronger by ..
  63. Zhao Y, SHI B, QIAN Y, BAI H, Xiao L, He X. Dynamic expression changes between non-muscle-invasive bladder cancer and muscle-invasive bladder cancer. Tumori. 2014;100:e273-81 pubmed publisher
    ..miR-29c and miR-9 were regarded as key microRNAs in MIBC. SMAD3 in MIBC and SMAD5 and SMAD7 in NMIBC were potential activated transcription factors...
  64. Canali S, Core A, Zumbrennen Bullough K, Merkulova M, Wang C, Schneyer A, et al. Activin B Induces Noncanonical SMAD1/5/8 Signaling via BMP Type I Receptors in Hepatocytes: Evidence for a Role in Hepcidin Induction by Inflammation in Male Mice. Endocrinology. 2016;157:1146-62 pubmed publisher
    ..ACVR2B, noncanonical BMP type I receptors activin receptor-like kinase 2 and activin receptor-like kinase 3, and SMAD5. The coreceptor hemojuvelin binds to activin B and facilitates activin B-SMAD1/5/8 signaling...
  65. Liu P, Wang C, Ma C, Wu Q, Zhang W, Lao G. MicroRNA-23a regulates epithelial-to-mesenchymal transition in endometrial endometrioid adenocarcinoma by targeting SMAD3. Cancer Cell Int. 2016;16:67 pubmed publisher
    ..The expression of SMAD3, SMAD5, and a panel of EMT markers was detected by Western blot and quantitative PCR...
  66. Wang C, Core A, Canali S, Zumbrennen Bullough K, Ozer S, Umans L, et al. Smad1/5 is required for erythropoietin-mediated suppression of hepcidin in mice. Blood. 2017;130:73-83 pubmed publisher
    ..In Hep3B cells, SMAD5 or SMAD1 but not SMAD8, knockdown inhibited hepcidin (HAMP) messenger RNA (mRNA) ..
  67. Gu Y, Ma L, Song L, Li X, Chen D, Bai X. miR-155 Inhibits Mouse Osteoblast Differentiation by Suppressing SMAD5 Expression. Biomed Res Int. 2017;2017:1893520 pubmed publisher
    ..The luciferase assay confirmed that miR-155 can bind to the 3' untranslated region of SMAD5 mRNA...
  68. Hejlik D, Kottickal L, Liang H, Fairman J, Davis T, Janecki T, et al. Localization of SMAD5 and its evaluation as a candidate myeloid tumor suppressor. Cancer Res. 1997;57:3779-83 pubmed
    ..4-Mb interval between the genes for IL9 and EGR1 on 5q31. In this report, we have localized the SMAD5 gene, a homologue of the tumor suppressor genes SMAD4/DPC-4 and SMAD2/JV18...
  69. Jeon E, Lee K, Choi N, Lee M, Kim H, Jin Y, et al. Bone morphogenetic protein-2 stimulates Runx2 acetylation. J Biol Chem. 2006;281:16502-11 pubmed
    ..These findings have important medical implications because BMPs and Runx2 are of tremendous interest with regard to the development of therapeutic agents against bone diseases. ..
  70. Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
    ..TGF? and BMP-activated receptor (r)-SMADs [phosphorylated SMAD2 or SMAD3 (pSMAD2,3), or phosphorylated SMAD1, SMAD5 or SMAD8 (pSMAD1,5,8)] undergo cell-cycle-dependent modulations and nucleo-cytosolic shuttling along the ..
  71. Fang T, Wu Q, Zhou L, Mu S, Fu Q. miR-106b-5p and miR-17-5p suppress osteogenic differentiation by targeting Smad5 and inhibit bone formation. Exp Cell Res. 2016;347:74-82 pubmed publisher
    Osteogenesis is a complex process which relies on the coordination of signals and transcription factors. BMP-2/Smad5 signal transduction pathway plays an important role in the process...
  72. Musto A, Navarra A, Vocca A, Gargiulo A, Minopoli G, Romano S, et al. miR-23a, miR-24 and miR-27a protect differentiating ESCs from BMP4-induced apoptosis. Cell Death Differ. 2015;22:1047-57 pubmed publisher
    ..This hypothesis is further supported by the observation that Smad5, the transcription factor downstream of the BMP4 receptor, is targeted by the miRNAs of the 23a and 23b clusters...
  73. Chai N, Li W, Wang J, Wang Z, Yang S, Wu J. Structural basis for the Smad5 MH1 domain to recognize different DNA sequences. Nucleic Acids Res. 2015;43:9051-64 pubmed publisher
    ..Here we report the first crystal structure of the Smad5 MH1 domain in complex with the GC-rich sequence...
  74. Hata A, Lagna G, Massague J, Hemmati Brivanlou A. Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Genes Dev. 1998;12:186-97 pubmed
    ..Smad6 specifically competes with Smad4 for binding to receptor-activated Smad1, yielding an apparently inactive Smad1-Smad6 complex. Therefore, Smad6 selectively antagonizes BMP-activated Smad1 by acting as a Smad4 decoy. ..
  75. Fuchs O, Simakova O, Klener P, Cmejlova J, Zivny J, Zavadil J, et al. Inhibition of Smad5 in human hematopoietic progenitors blocks erythroid differentiation induced by BMP4. Blood Cells Mol Dis. 2002;28:221-33 pubmed
    ..interstitial deletions of the chromosome-5q resulting in hemizygous loss of the transcription transactivator Smad5. Smad5 is a member of the signal transducer family conveying the pleiotropic TGF-gb/BMP cytokine signals with ..
  76. Zhang N, Wang D, Zhu Y, Wang J, Lin H. Inhibition effects of lamellarin D on human leukemia K562 cell proliferation and underlying mechanisms. Asian Pac J Cancer Prev. 2014;15:9915-9 pubmed
    ..cell proliferation and induction of G0/G1-phase arrest,while expression of CDK1, and activity of smad3 and smad5 were reduced, but that of p27, p53 and STGC3 was increased...
  77. Zuo Q, Jin K, Zhang Y, Song J, Li B. Dynamic expression and regulatory mechanism of TGF-β signaling in chicken embryonic stem cells differentiating into spermatogonial stem cells. Biosci Rep. 2017;37: pubmed publisher
    ..of ESCs in vitro Inhibition of TGF-β signaling pathway was reflected by Western blot of SMAD2 and SMAD5 expression...
  78. Lewis M, McAndrew M, Wheeler C, Workman N, Agashe P, Koopmann J, et al. Autoantibodies targeting TLR and SMAD pathways define new subgroups in systemic lupus erythematosus. J Autoimmun. 2018;91:1-12 pubmed publisher
    ..Two clusters of novel autoantigens revealed distinctive networks of interacting proteins: SMAD2, SMAD5 and proteins linked to TGF-β signalling; and MyD88 and proteins involved in TLR signalling, apoptosis, NF-κB ..
  79. Jiao K, Zhou Y, Hogan B. Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1. Mol Cell Biol. 2002;22:7633-44 pubmed
    ..Our data support the hypothesis that mZnf8 plays critical roles in mediating BMP signaling during spermatogenesis. ..
  80. Tripurani S, Cook R, Eldin K, Pangas S. BMP-specific SMADs function as novel repressors of PDGFA and modulate its expression in ovarian granulosa cells and tumors. Oncogene. 2013;32:3877-85 pubmed publisher
    ..that conditional deletion of two transcription factors that signal for the bone morphogenetic proteins (Smad1 and Smad5) in ovarian granulosa cells causes metastatic granulosa cell tumors (GCTs) in female mice and phenocopies human ..
  81. Zhu L, Ma J, Mu R, Zhu R, Chen F, Wei X, et al. Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro. Life Sci. 2018;202:175-181 pubmed publisher
    ..Mineralization of DPSCs was evaluated by alizarin red staining. The Smad5 signaling pathway was examined by qRT-PCR and western blot analysis...
  82. Scollen S, Luccarini C, Baynes C, Driver K, Humphreys M, Garcia Closas M, et al. TGF-β signaling pathway and breast cancer susceptibility. Cancer Epidemiol Biomarkers Prev. 2011;20:1112-9 pubmed publisher
    ..LTBP1, LTBP2, LTBP4, TGFB1, TGFB2, TGFB3, TGFBR1(ALK5), ALK1, TGFBR2, Endoglin, SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6, and SMAD7 [Approved Human Gene Nomenclature Committee gene names: ACVRL1 (for ALK1) and ENG (for Endoglin)]..
  83. Xu S, Cecilia Santini G, De Veirman K, Vande Broek I, Leleu X, De Becker A, et al. Upregulation of miR-135b is involved in the impaired osteogenic differentiation of mesenchymal stem cells derived from multiple myeloma patients. PLoS ONE. 2013;8:e79752 pubmed publisher
    ..an abnormal upregulation of miR-135b, showing meanwhile an impaired osteogenic differentiation and a decrease of SMAD5 expression, which is the target of miR-135b involved in osteogenesis...
  84. Shang M, Su B, Lipke E, Perera D, Li C, Qin Z, et al. Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus. Fish Physiol Biochem. 2015;41:1545-56 pubmed publisher
    ..In contrast to channel catfish, Id4, Smad5 and Prdm14 gene expressions were strongly down-regulated in spermatocyte cells, but up-regulated in spermatid ..
  85. Daher R, Kannengiesser C, Houamel D, Lefebvre T, Bardou Jacquet E, Ducrot N, et al. Heterozygous Mutations in BMP6 Pro-peptide Lead to Inappropriate Hepcidin Synthesis and Moderate Iron Overload in Humans. Gastroenterology. 2016;150:672-683.e4 pubmed publisher
    ..the mutated residues in the BMP6 propeptide resulted in defective secretion of BMP6; reduced signaling via SMAD1, SMAD5, and SMAD8; and loss of hepcidin production...
  86. Dong Z, Tai W, Lei W, Wang Y, Li Z, Zhang T. IL-27 inhibits the TGF-β1-induced epithelial-mesenchymal transition in alveolar epithelial cells. BMC Cell Biol. 2016;17:7 pubmed publisher
    ..Furthermore, we found that TGF-β1 activated the phosphorylation of JAK1, STAT1, STAT3, STAT5, Smad1, Smad3 and Smad5, and IL-27 partially inhibited these changes in this process...
  87. Huang B, Lu J, Ding C, Zou Q, Wang W, Li H. Exosomes derived from human adipose mesenchymal stem cells improve ovary function of premature ovarian insufficiency by targeting SMAD. Stem Cell Res Ther. 2018;9:216 pubmed publisher
    ..PCR and western blot assays were used to determine the mRNA and protein expression levels of SMAD2, SMAD3, and SMAD5. Western blot assays were used to test the protein expression levels of apoptosis genes (Fas, FasL, caspase-3, and ..
  88. Hegarty S, O Keeffe G, Sullivan A. BMP-Smad 1/5/8 signalling in the development of the nervous system. Prog Neurobiol. 2013;109:28-41 pubmed publisher
    The transcription factors, Smad1, Smad5 and Smad8, are the pivotal intracellular effectors of the bone morphogenetic protein (BMP) family of proteins...
  89. Kersten C, Sivertsen E, Hystad M, Forfang L, Smeland E, Myklebust J. BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1. BMC Immunol. 2005;6:9 pubmed
    ..Furthermore, B cells were demonstrated to upregulate BMP-6 mRNA upon stimulation with anti-IgM. In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad1/5/8 followed by an upregulation of Id1. ..
  90. Attisano L, Silvestri C, Izzi L, Labbe E. The transcriptional role of Smads and FAST (FoxH1) in TGFbeta and activin signalling. Mol Cell Endocrinol. 2001;180:3-11 pubmed
    ..Thus, it is the interaction of Smads with a wide range of specific transcriptional partners that is important for the generation of diverse biological responses to TGFbeta superfamily members. ..
  91. Xiao W, Li Y, Lou W, Cai T, Zhang S, Hu X, et al. MicroRNA-93-5p may participate in the formation of morphine tolerance in bone cancer pain mouse model by targeting Smad5. Oncotarget. 2016;7:52104-52114 pubmed publisher
    In this study, we aim to find out the role of microRNA-93-5p (miR-93) and Smad5 in morphine tolerance in mouse models of bone cancer pain (BCP)...
  92. Sinha P, Tesfaye D, Rings F, Hossien M, Hoelker M, Held E, et al. MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation. J Ovarian Res. 2017;10:37 pubmed publisher
    ..The luciferase assay showed that SMAD5 and MSK1 genes were identified as the direct targets of miR-130b...