Gene Symbol: SLC9A3R1
Description: SLC9A3 regulator 1
Alias: EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, ezrin-radixin-moesin binding phosphoprotein-50, regulatory cofactor of Na(+)/H(+) exchanger, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1, solute carrier family 9 isoform A3 regulatory factor 1, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Species: human
Products:     SLC9A3R1

Top Publications

  1. Weinman E, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S. A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange. Biochemistry. 2003;42:12662-8 pubmed
    b>NHERF-1, a protein adapter containing two tandem PDZ domains, was first identified as an essential cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP...
  2. Mahon M, Donowitz M, Yun C, Segre G. Na(+)/H(+ ) exchanger regulatory factor 2 directs parathyroid hormone 1 receptor signalling. Nature. 2002;417:858-61 pubmed
    ..Here we show that the PTH1R binds to Na(+)/H(+) exchanger regulatory factors (NHERF) 1 and 2 through a PDZ-domain interaction in vitro and in PTH target cells...
  3. Reczek D, Bretscher A. Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP. J Cell Biol. 2001;153:191-206 pubmed
    The cortical scaffolding proteins EBP50 (ERM-binding phosphoprotein-50) and E3KARP (NHE3 kinase A regulatory protein) contain two PDZ (PSD-95/DlgA/ZO-1-like) domains followed by a COOH-terminal sequence that binds to active ERM family ..
  4. Short D, Trotter K, Reczek D, Kreda S, Bretscher A, Boucher R, et al. An apical PDZ protein anchors the cystic fibrosis transmembrane conductance regulator to the cytoskeleton. J Biol Chem. 1998;273:19797-801 pubmed
    ..Here we report that CFTR interacts with the recently discovered protein, EBP50 (ERM-binding phosphoprotein 50)...
  5. Mangia A, Saponaro C, Malfettone A, Bisceglie D, Bellizzi A, Asselti M, et al. Involvement of nuclear NHERF1 in colorectal cancer progression. Oncol Rep. 2012;28:889-94 pubmed publisher
    b>NHERF1 (Na+/H+ exchanger regulatory factor 1) is expressed in the luminal membrane of many epithelia, and associated with proteins involved in tumor progression...
  6. Yun C, Oh S, Zizak M, Steplock D, Tsao S, Tse C, et al. cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein. Proc Natl Acad Sci U S A. 1997;94:3010-5 pubmed
    ..We have identified two regulatory proteins, E3KARP and NHERF, that interact with NHE3 to enable cAMP to inhibit NHE3...
  7. Lin Y, Hsu Y, Huang H, Shann Y, Huang C, Wei S, et al. Aberrant nuclear localization of EBP50 promotes colorectal carcinogenesis in xenotransplanted mice by modulating TCF-1 and ?-catenin interactions. J Clin Invest. 2012;122:1881-94 pubmed publisher
    ..Here, we show that ERM-binding phosphoprotein 50 (EBP50) modulates the interaction between ?-catenin and TCF-1...
  8. Karthikeyan S, Leung T, Birrane G, Webster G, Ladias J. Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains. J Mol Biol. 2001;308:963-73 pubmed
    The Na(+)/H(+) exchanger regulatory factor (NHERF; also known as EBP50) contains two PDZ domains that mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes...
  9. Li J, Dai Z, Jana D, Callaway D, Bu Z. Ezrin controls the macromolecular complexes formed between an adapter protein Na+/H+ exchanger regulatory factor and the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 2005;280:37634-43 pubmed
    Na(+)/H(+) exchanger regulatory factor (NHERF) is an adapter protein that is responsible for organizing a number of cell receptors and channels...

More Information


  1. Dai J, Wang L, Sahin A, Broemeling L, Schutte M, Pan Y. NHERF (Na+/H+ exchanger regulatory factor) gene mutations in human breast cancer. Oncogene. 2004;23:8681-7 pubmed
    ..The screening yielded NHERF (Na+/H+ exchanger regulatory factor, also known as NHERF1 or EBP-50) that binds to the interdomain B of SYK...
  2. Wheeler D, Barrick S, Grubisha M, Brufsky A, Friedman P, Romero G. Direct interaction between NHERF1 and Frizzled regulates ?-catenin signaling. Oncogene. 2011;30:32-42 pubmed publisher
    ..We show here that the adaptor protein Na(+)/H(+) exchange regulatory factor1 (NHERF1), a protein abundantly expressed in normal mammary epithelium, regulates Wnt signaling, maintaining low levels of ?..
  3. Lee Kwon W, Kim J, Choi J, Kawano K, Cha B, Dartt D, et al. Ca2+-dependent inhibition of NHE3 requires PKC alpha which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes. Am J Physiol Cell Physiol. 2003;285:C1527-36 pubmed
    ..These results suggest that PKCalpha is not necessary for the Ca2+-dependent formation of the NHE3 plasma membrane complex, although it is necessary for decreasing the membrane amounts of NHE3, probably by stimulating NHE3 endocytosis. ..
  4. Shibata T, Chuma M, Kokubu A, Sakamoto M, Hirohashi S. EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma. Hepatology. 2003;38:178-86 pubmed
    ..By protein interaction screening, we identified EBP50, a cytoplasmic protein with 2 PDZ domains, as a beta-catenin-associating molecule...
  5. Kislin K, McDonough W, Eschbacher J, Armstrong B, Berens M. NHERF-1: modulator of glioblastoma cell migration and invasion. Neoplasia. 2009;11:377-87 pubmed
    ..Na(+)/H(+) exchanger regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein, recruiting cytoplasmic signaling proteins and membrane receptors/..
  6. Hayashi Y, Molina J, Hamilton S, Georgescu M. NHERF1/EBP50 is a new marker in colorectal cancer. Neoplasia. 2010;12:1013-22 pubmed
    ..We report here that Na(+)/H(+) exchanger 3 regulating factor 1 (NHERF1)/EBP50, an adaptor molecule that interacts with ?-catenin, undergoes successive alterations during the colorectal ..
  7. Li J, Poulikakos P, Dai Z, Testa J, Callaway D, Bu Z. Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly. J Biol Chem. 2007;282:27086-99 pubmed
    ..kinase C (PKC) phosphorylation influences the scaffolding protein Na(+)/H(+) exchanger regulatory factor 1 (NHERF) to assemble protein complexes of cystic fibrosis transmembrane conductance regulator (CFTR), a chloride ion ..
  8. Molina J, Agarwal N, Morales F, Hayashi Y, Aldape K, Cote G, et al. PTEN, NHERF1 and PHLPP form a tumor suppressor network that is disabled in glioblastoma. Oncogene. 2012;31:1264-74 pubmed publisher
    ..pathway in glioblastoma within a tumor suppressor network that includes Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) and pleckstrin-homology domain leucine-rich repeat protein phosphatases 1 (PHLPP1)...
  9. Bellizzi A, Mangia A, Malfettone A, Cardone R, Simone G, Reshkin S, et al. Na+/H+ exchanger regulatory factor 1 expression levels in blood and tissue predict breast tumour clinical behaviour. Histopathology. 2011;58:1086-95 pubmed publisher
    Several studies have demonstrated that Na(+) /H(+) exchanger regulatory factor 1 (NHERF1) protein, which is overexpressed and heterogeneously distributed in different stages of breast cancer, could be used as a tumour marker for prognosis ..
  10. Zheng J, Sun L, Liu H, Huang Y, Li Y, He J. EBP50 exerts tumor suppressor activity by promoting cell apoptosis and retarding extracellular signal-regulated kinase activity. Amino Acids. 2010;38:1261-8 pubmed publisher
    The expression of Ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) and the intragenic mutation of the ebp50 gene have been reported to correlate with human breast cancer development, but the exact impacts on breast cancer ..
  11. Ardura J, Wang B, Watkins S, Vilardaga J, Friedman P. Dynamic Na+-H+ exchanger regulatory factor-1 association and dissociation regulate parathyroid hormone receptor trafficking at membrane microdomains. J Biol Chem. 2011;286:35020-9 pubmed publisher
    Na/H exchanger regulatory factor-1 (NHERF1) is a cytoplasmic PDZ (postsynaptic density 95/disc large/zona occludens) protein that assembles macromolecular complexes and determines the localization, trafficking, and signaling of select G ..
  12. Karn T, Ruckhäberle E, Hanker L, Muller V, Schmidt M, Solbach C, et al. Gene expression profiling of luminal B breast cancers reveals NHERF1 as a new marker of endocrine resistance. Breast Cancer Res Treat. 2011;130:409-20 pubmed publisher
    ..The clinical relevance of the scaffold protein NHERF1 identified by this strategy was assessed in a total of 3,030 breast cancers...
  13. James M, Beauchamp R, Manchanda N, Kazlauskas A, Ramesh V. A NHERF binding site links the betaPDGFR to the cytoskeleton and regulates cell spreading and migration. J Cell Sci. 2004;117:2951-61 pubmed
    The Na(+)/H(+) exchanger regulatory factor, NHERF, is a multifunctional adapter protein involved in a wide range of physiological activities...
  14. Weinman E, Steplock D, Zhang Y, Biswas R, Bloch R, Shenolikar S. Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport. J Biol Chem. 2010;285:25134-8 pubmed publisher
    The phosphorylation of the sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) plays a key role in the regulation of renal phosphate transport by parathyroid hormone (PTH) and dopamine...
  15. Malfettone A, Silvestris N, Paradiso A, Mattioli E, Simone G, Mangia A. Overexpression of nuclear NHERF1 in advanced colorectal cancer: association with hypoxic microenvironment and tumor invasive phenotype. Exp Mol Pathol. 2012;92:296-303 pubmed publisher
    ..The present study investigates protein expression of NHERF1, HIF-1? and TWIST1 and their relationship in distant normal mucosa (DNM), tumor (T) and adjacent normal mucosa (..
  16. Wang B, Yang Y, Abou Samra A, Friedman P. NHERF1 regulates parathyroid hormone receptor desensitization: interference with beta-arrestin binding. Mol Pharmacol. 2009;75:1189-97 pubmed publisher
    ..The Na(+)/H(+) exchange regulatory factor 1 (NHERF1) is a cytoplasmic adapter protein that regulates trafficking and signaling of several G protein-coupled receptors (..
  17. Cardone R, Bellizzi A, Busco G, Weinman E, Dell Aquila M, Casavola V, et al. The NHERF1 PDZ2 domain regulates PKA-RhoA-p38-mediated NHE1 activation and invasion in breast tumor cells. Mol Biol Cell. 2007;18:1768-80 pubmed
    ..Na(+)/H(+) exchanger regulatory factor (NHERF1) is a postsynaptic density 95/disc-large/zona occludens (PDZ) domain-containing protein that recruits membrane ..
  18. Hammad M, Kuang Y, Yan R, Allen H, Dupre D. Na+/H+ exchanger regulatory factor-1 is involved in chemokine receptor homodimer CCR5 internalization and signal transduction but does not affect CXCR4 homodimer or CXCR4-CCR5 heterodimer. J Biol Chem. 2010;285:34653-64 pubmed publisher
    ..We identified a receptor adaptor interacting specifically with one receptor complex but not others. NHERF1, an adaptor known for its role in desensitization, internalization, and regulation of the ERK signaling cascade ..
  19. Breton S, Wiederhold T, Marshansky V, Nsumu N, Ramesh V, Brown D. The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells. J Biol Chem. 2000;275:18219-24 pubmed
    ..The colocalization of NHE-RF and H(+)ATPase in B- but not A-intercalated cells suggests a role in generating, maintaining, or modulating the variable H(+)ATPase polarity that characterizes the B-cell phenotype. ..
  20. Zhou F, Xu W, Tanaka K, You G. Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells. Pharm Res. 2008;25:475-80 pubmed
    ..PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks ..
  21. Ediger T, Park S, Katzenellenbogen B. Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements. Mol Endocrinol. 2002;16:1828-39 pubmed
    ..Our findings highlight a paradigm for gene regulation via numerous half-ERE sites that expands the range of modes by which DNA recognition sites mediate the actions of this nuclear receptor. ..
  22. Hall R, Spurney R, Premont R, Rahman N, Blitzer J, Pitcher J, et al. G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+) exchanger regulatory factor via a PDZ domain-mediated interaction. J Biol Chem. 1999;274:24328-34 pubmed
    The Na(+)/H(+) exchanger regulatory factor (NHERF) is constitutively phosphorylated in cells, but the site(s) of this phosphorylation and the kinase(s) responsible for it have not been identified...
  23. Cushing P, Fellows A, Villone D, Boisguerin P, Madden D. The relative binding affinities of PDZ partners for CFTR: a biochemical basis for efficient endocytic recycling. Biochemistry. 2008;47:10084-98 pubmed publisher
    ..competition between CAL and other PDZ proteins including Na (+)/H (+) exchanger-3 regulatory factors 1 and 2 (NHERF1 and NHERF2), which functionally stabilize cell-surface CFTR...
  24. Karthikeyan S, Leung T, Ladias J. Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors. J Biol Chem. 2002;277:18973-8 pubmed
    The Na(+)/H(+) exchanger regulatory factor (NHERF) binds through its PDZ1 domain to the carboxyl-terminal sequences NDSLL and EDSFL of the beta(2) adrenergic receptor (beta(2)AR) and platelet-derived growth factor receptor, respectively, ..
  25. Claperon A, Guedj N, Mergey M, Vignjevic D, Desbois Mouthon C, Boissan M, et al. Loss of EBP50 stimulates EGFR activity to induce EMT phenotypic features in biliary cancer cells. Oncogene. 2012;31:1376-88 pubmed publisher
    ..The scaffold protein ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is highly expressed at the plasma membrane of normal biliary epithelial cells and binds epidermal growth factor ..
  26. Mangia A, Chiriatti A, Bellizzi A, Malfettone A, Stea B, Zito F, et al. Biological role of NHERF1 protein expression in breast cancer. Histopathology. 2009;55:600-8 pubmed publisher
    To determine the role of Na+/H+ exchanger regulatory factor (NHERF1) in breast cancerogenesis and progression...
  27. Takahashi Y, Morales F, Kreimann E, Georgescu M. PTEN tumor suppressor associates with NHERF proteins to attenuate PDGF receptor signaling. EMBO J. 2006;25:910-20 pubmed
    ..We show here that PTEN interacts directly with the NHERF1 and NHERF2 (Na+/H+ exchanger regulatory factor) homologous adaptor proteins through the PDZ motif of PTEN and the ..
  28. Weinman E, Steplock D, Wang Y, Shenolikar S. Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger. J Clin Invest. 1995;95:2143-9 pubmed
    ..Reverse transcription/PCR studies in the kidney indicate the presence of mRNA for NHE-RF in several distinct nephron segments including the proximal tubule. ..
  29. Huang P, Steplock D, Weinman E, Hall R, Ding Z, Li J, et al. kappa Opioid receptor interacts with Na(+)/H(+)-exchanger regulatory factor-1/Ezrin-radixin-moesin-binding phosphoprotein-50 (NHERF-1/EBP50) to stimulate Na(+)/H(+) exchange independent of G(i)/G(o) proteins. J Biol Chem. 2004;279:25002-9 pubmed
    ..showed that Na(+)/H(+)-exchanger regulatory factor-1/Ezrin-radixin-moesin-binding phosphoprotein-50 (NHERF-1/EBP50) co-immunoprecipitated with the human kappa opioid receptor (hKOR) and that its overexpression blocked the kappa ..
  30. Li J, Callaway D, Bu Z. Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1. J Mol Biol. 2009;392:166-80 pubmed publisher
    ..The scaffolding protein Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) assembles macromolecular signaling complexes and regulates the macromolecular assembly, localization, and ..
  31. Tang Y, Tang J, Chen Z, Trost C, Flockerzi V, Li M, et al. Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF. J Biol Chem. 2000;275:37559-64 pubmed that murine Trp4 and Trp5, as well as phospholipase C beta1 and beta2 interact with the first PDZ domain of NHERF, regulatory factor of the Na(+)/H(+) exchanger...
  32. Song J, Bai J, Yang W, Gabrielson E, Chan D, Zhang Z. Expression and clinicopathological significance of oestrogen-responsive ezrin-radixin-moesin-binding phosphoprotein 50 in breast cancer. Histopathology. 2007;51:40-53 pubmed
    Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a post synaptic density-95/Disk-large/ZO-1 homologous domain-containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton and plays an ..
  33. Kato Y, Sai Y, Yoshida K, Watanabe C, Hirata T, Tsuji A. PDZK1 directly regulates the function of organic cation/carnitine transporter OCTN2. Mol Pharmacol. 2005;67:734-43 pubmed
    ..The present findings are the first to identify PDZK1 as a functional regulator of OCTN2 through direct interaction with the C terminus. ..
  34. Morales F, Takahashi Y, Momin S, Adams H, Chen X, Georgescu M. NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Mol Cell Biol. 2007;27:2527-37 pubmed
    ..We describe here an intramolecular conformation of NHERF1/EBP50 (ERM-binding phosphoprotein 50) in which the C-terminal EB region binds to the PDZ2 domain...
  35. Miyazaki H, Anzai N, Ekaratanawong S, Sakata T, Shin H, Jutabha P, et al. Modulation of renal apical organic anion transporter 4 function by two PDZ domain-containing proteins. J Am Soc Nephrol. 2005;16:3498-506 pubmed
    ..Here, two multivalent PDZ (PSD-95/Discs Large/ZO-1) proteins PDZK1 and NHERF1 were examined as interactors of OAT4 by a yeast two-hybrid assay...
  36. Helms C, Cao L, Krueger J, Wijsman E, Chamian F, Gordon D, et al. A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis. Nat Genet. 2003;35:349-56 pubmed
    ..Associated single-nucleotide polymorphisms (SNPs) in the proximal peak lie in or near SLC9A3R1 (also called EBP50 and NHERF1) and NAT9, a new member of the N-acetyltransferase family...
  37. Terawaki S, Maesaki R, Hakoshima T. Structural basis for NHERF recognition by ERM proteins. Structure. 2006;14:777-89 pubmed
    The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein involved in the anchoring of ion channels and receptors to the actin cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins...
  38. Fouassier L, Rosenberg P, Mergey M, Saubamea B, Clapéron A, Kinnman N, et al. Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation. Am J Pathol. 2009;174:869-80 pubmed publisher
    Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) anchors and regulates apical membrane proteins in epithelia. EBP50 is inducible by estrogen and may affect cell proliferation, although this latter function remains unclear...
  39. Garbett D, LaLonde D, Bretscher A. The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. J Cell Biol. 2010;191:397-413 pubmed publisher
    ..A potential regulator is EBP50/NHERF1 (ERM-binding phosphoprotein of 50 kD/Na(+)-H(+) exchanger regulatory factor), a microvillar scaffolding protein ..
  40. Cao T, Deacon H, Reczek D, Bretscher A, von Zastrow M. A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor. Nature. 1999;401:286-90 pubmed
    ..The phosphoprotein EBP50 (for ezrinradixin-moesin(ERM)-binding phosphoprotein-50) binds to the cytoplasmic tail of the beta2-adrenergic ..
  41. Accardi R, Rubino R, Scalise M, Gheit T, Shahzad N, Thomas M, et al. E6 and E7 from human papillomavirus type 16 cooperate to target the PDZ protein Na/H exchange regulatory factor 1. J Virol. 2011;85:8208-16 pubmed publisher
    ..a novel interaction of HPV type 16 (HPV16) E6 with a PDZ protein, Na(+)/H(+) exchange regulatory factor 1 (NHERF-1), which is involved in a number of cellular processes, including signaling and transformation...
  42. Gisler S, Stagljar I, Traebert M, Bacic D, Biber J, Murer H. Interaction of the type IIa Na/Pi cotransporter with PDZ proteins. J Biol Chem. 2001;276:9206-13 pubmed
    ..found belonged to proteins with multiple PDZ modules and were either identical/related to PDZK1 or identical to NHERF-1...
  43. Wang B, Bisello A, Yang Y, Romero G, Friedman P. NHERF1 regulates parathyroid hormone receptor membrane retention without affecting recycling. J Biol Chem. 2007;282:36214-22 pubmed
    Na/H exchange regulatory factor-1 (NHERF1) is a PDZ protein that regulates trafficking of several G protein-coupled receptors...
  44. Bhattacharya S, Ju J, Orlova N, Khajeh J, Cowburn D, Bu Z. Ligand-induced dynamic changes in extended PDZ domains from NHERF1. J Mol Biol. 2013;425:2509-28 pubmed publisher
    The multi-domain scaffolding protein NHERF1 modulates the assembly and intracellular trafficking of various transmembrane receptors and ion-transport proteins...
  45. Molina J, Morales F, Hayashi Y, Aldape K, Georgescu M. Loss of PTEN binding adapter protein NHERF1 from plasma membrane in glioblastoma contributes to PTEN inactivation. Cancer Res. 2010;70:6697-703 pubmed publisher
    ..The PTEN binding adapter protein NHERF1/EBP50 is overexpressed in GBM but its effects on tumorigenesis have yet to be determined...
  46. LaLonde D, Bretscher A. The scaffold protein PDZK1 undergoes a head-to-tail intramolecular association that negatively regulates its interaction with EBP50. Biochemistry. 2009;48:2261-71 pubmed publisher
    ..A head-to-tail association has also been reported for EBP50/NHERF1, a two-PDZ domain member of the same scaffolding protein family as PDZK1, and shown to regulate binding of target ..
  47. Voltz J, Brush M, Sikes S, Steplock D, Weinman E, Shenolikar S. Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets. J Biol Chem. 2007;282:33879-87 pubmed
    NHERF-1 (Na(+)-H(+) exchanger regulatory factor 1, also known as EBP50 ezrin-binding protein of 50 kDa) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain...
  48. Raghuram V, Hormuth H, Foskett J. A kinase-regulated mechanism controls CFTR channel gating by disrupting bivalent PDZ domain interactions. Proc Natl Acad Sci U S A. 2003;100:9620-5 pubmed
    ..The Na+/H+ exchanger regulatory factor (NHERF) binds to CFTR and increases its open probability (Po)...
  49. Garbett D, Bretscher A. PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli. J Cell Biol. 2012;198:195-203 pubmed publisher
    ..The microvillar scaffolding protein EBP50 (ERM-binding phosphoprotein of 50 kD), consisting of two PDZ domains and an ezrin-binding site, retains specific ..
  50. Hall R, Premont R, Chow C, Blitzer J, Pitcher J, Claing A, et al. The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange. Nature. 1998;392:626-30 pubmed agonist-promoted association of the beta2-adrenergic receptor with the Na+/H+ exchanger regulatory factor (NHERF), a protein that regulates the activity of the Na+/H+ exchanger type 3 (NHE3)...
  51. Finnerty C, Chambers D, Ingraffea J, Faber H, Karplus P, Bretscher A. The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain. J Cell Sci. 2004;117:1547-52 pubmed
    ..crosslinking proteins that, upon activation, bind the scaffolding protein ERM-phosphoprotein of 50 kDa (EBP50). Here we report a 3...
  52. Zhang Q, Pan Z, You G. Regulation of human organic anion transporter 4 by protein kinase C and NHERF-1: altering the endocytosis of the transporter. Pharm Res. 2010;27:589-96 pubmed publisher
    ..shown that the activity of hOAT4 was down-regulated by activation of PKC and up-regulated by PDZ protein NHERF-1. Here, we investigated the mechanisms underlying such regulations...
  53. Cardone R, Greco M, Capulli M, Weinman E, Busco G, Bellizzi A, et al. NHERF1 acts as a molecular switch to program metastatic behavior and organotropism via its PDZ domains. Mol Biol Cell. 2012;23:2028-40 pubmed publisher
    ..Na(+)/H(+) exchanger regulatory factor (NHERF1) is a molecular pathway organizer, PDZ-domain protein that recruits membrane, cytoplasmic, and cytoskeletal ..
  54. Pan Y, Wang L, Dai J. Suppression of breast cancer cell growth by Na+/H+ exchanger regulatory factor 1 (NHERF1). Breast Cancer Res. 2006;8:R63 pubmed
    Na+/H+ exchanger regulatory factor 1 (NHERF1, also known as EBP50 or NHERF) is a putative tumour suppressor gene in human breast cancer. Located at 17q25.1, NHERF1 is frequently targeted during breast tumourigenesis...
  55. Karthikeyan S, Leung T, Ladias J. Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 2001;276:19683-6 pubmed
    The PDZ1 domain of the Na(+)/H(+) exchanger regulatory factor (NHERF) binds with nanomolar affinity to the carboxyl-terminal sequence QDTRL of the cystic fibrosis transmembrane conductance regulator (CFTR) and plays a central role in the ..
  56. Sarker R, Valkhoff V, Zachos N, Lin R, Cha B, Chen T, et al. NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity. Am J Physiol Cell Physiol. 2011;300:C771-82 pubmed publisher
    ..Most studies of NHE3 regulation have been in cell models in which NHERF1 and/or NHERF2 were overexpressed...
  57. Ingraffea J, Reczek D, Bretscher A. Distinct cell type-specific expression of scaffolding proteins EBP50 and E3KARP: EBP50 is generally expressed with ezrin in specific epithelia, whereas E3KARP is not. Eur J Cell Biol. 2002;81:61-8 pubmed
    ..b>EBP50 and E3KARP are two related scaffolding proteins that bind to the activated form of ERM proteins in vitro, and ..
  58. Hall R, Ostedgaard L, Premont R, Blitzer J, Rahman N, Welsh M, et al. A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins. Proc Natl Acad Sci U S A. 1998;95:8496-501 pubmed
    The Na+/H+ exchanger regulatory factor (NHERF) binds to the tail of the beta2-adrenergic receptor and plays a role in adrenergic regulation of Na+/H+ exchange...
  59. Maudsley S, Zamah A, Rahman N, Blitzer J, Luttrell L, Lefkowitz R, et al. Platelet-derived growth factor receptor association with Na(+)/H(+) exchanger regulatory factor potentiates receptor activity. Mol Cell Biol. 2000;20:8352-63 pubmed
    ..We show here that the Na(+)/H(+) exchanger regulatory factor (NHERF; also known as EBP50), a protein not previously known to interact with the PDGFR, binds to the PDGFR carboxyl terminus (PDGFR-CT) with ..
  60. Raghuram V, Mak D, Foskett J. Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction. Proc Natl Acad Sci U S A. 2001;98:1300-5 pubmed
    ..The Na(+)/H(+) exchanger regulatory factor (NHERF) binds to the cytoplasmic tail of CFTR through either of its two PDZ (PDZ1 and PDZ2) domains...
  61. Mangia A, Malfettone A, Saponaro C, Tommasi S, Simone G, Paradiso A. Human epidermal growth factor receptor 2, Na+/H+ exchanger regulatory factor 1, and breast cancer susceptibility gene-1 as new biomarkers for familial breast cancers. Hum Pathol. 2011;42:1589-95 pubmed publisher
  62. Karim Z, Gerard B, Bakouh N, Alili R, Leroy C, Beck L, et al. NHERF1 mutations and responsiveness of renal parathyroid hormone. N Engl J Med. 2008;359:1128-35 pubmed publisher
    ..Data from animal models suggest that sodium-hydrogen exchanger regulatory factor 1 (NHERF1) controls renal phosphate transport...
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    PDZK1 and ezrin, radixin, moesin binding phosphoprotein 50 kDa (EBP50) are postsynaptic density 95/disc-large/zona occludens (PDZ)-domain-containing scaffolding proteins found in the apical microvilli of polarized epithelial cells...
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    ..of the cystic fibrosis transmembrane conductance regulator associates with the submembranous scaffolding protein EBP50 (ERM-binding phosphoprotein 50 kD; also called Na(+)/H(+) exchanger regulatory factor)...
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    The Na/H exchanger regulatory factors, NHERF1 and NHERF2, are adapter proteins involved in targeting and assembly of protein complexes. The parathyroid hormone receptor (PTHR) interacts with both NHERF1 and NHERF2...