SLC46A1

Summary

Gene Symbol: SLC46A1
Description: solute carrier family 46 (folate transporter), member 1
Alias: G21, HCP1, PCFT, heme carrier protein 1, proton-coupled folate transporter
Species: human

Top Publications

  1. ncbi Identification of an intestinal heme transporter
    Majid Shayeghi
    Department of Life Sciences, Nutritional Sciences Research Division, Franklin Wilkins Building, Kings College London, 150 Stamford Street, London SE1 9NN, UK
    Cell 122:789-801. 2005
  2. pmc A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis
    Rongbao Zhao
    Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 284:4267-74. 2009
  3. ncbi Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell 127:917-28. 2006
  4. pmc Functional roles of aspartate residues of the proton-coupled folate transporter (PCFT-SLC46A1); a D156Y mutation causing hereditary folate malabsorption
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA
    Blood 116:5162-9. 2010
  5. pmc Identification and functional impact of homo-oligomers of the human proton-coupled folate transporter
    Zhanjun Hou
    Developmental Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 287:4982-95. 2012
  6. pmc Identification of a functionally critical GXXG motif and its relationship to the folate binding site of the proton-coupled folate transporter (PCFT-SLC46A1)
    Rongbao Zhao
    Depts of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Cell Physiol 303:C673-81. 2012
  7. pmc The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption
    Rongbao Zhao
    Departments of Medicine, Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood 110:1147-52. 2007
  8. pmc A P425R mutation of the proton-coupled folate transporter causing hereditary folate malabsorption produces a highly selective alteration in folate binding
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 302:C1405-12. 2012
  9. doi Functional elements in the minimal promoter of the human proton-coupled folate transporter
    Michal Stark
    Dept of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Biochem Biophys Res Commun 388:79-85. 2009
  10. pmc Role of the glutamate 185 residue in proton translocation mediated by the proton-coupled folate transporter SLC46A1
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 297:C66-74. 2009

Research Grants

Scientific Experts

Detail Information

Publications113 found, 100 shown here

  1. ncbi Identification of an intestinal heme transporter
    Majid Shayeghi
    Department of Life Sciences, Nutritional Sciences Research Division, Franklin Wilkins Building, Kings College London, 150 Stamford Street, London SE1 9NN, UK
    Cell 122:789-801. 2005
    ..Here we show a membrane protein named HCP 1 (heme carrier protein 1), with homology to bacterial metal-tetracycline transporters, mediates heme uptake by cells in a ..
  2. pmc A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis
    Rongbao Zhao
    Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 284:4267-74. 2009
    Recently, this laboratory identified a proton-coupled folate transporter (PCFT), with optimal activity at low pH...
  3. ncbi Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell 127:917-28. 2006
    ..This transporter was previously reported to be a lower-affinity, pH-independent heme carrier protein, HCP1. However, the current study establishes that a major function of this gene product is proton-coupled folate ..
  4. pmc Functional roles of aspartate residues of the proton-coupled folate transporter (PCFT-SLC46A1); a D156Y mutation causing hereditary folate malabsorption
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA
    Blood 116:5162-9. 2010
    The proton-coupled folate transporter (PCFT; SLC46A1) mediates folate transport into enterocytes in the proximal small intestine; pcft loss-of-function mutations are the basis for hereditary folate malabsorption...
  5. pmc Identification and functional impact of homo-oligomers of the human proton-coupled folate transporter
    Zhanjun Hou
    Developmental Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 287:4982-95. 2012
    The proton-coupled folate transporter (PCFT; SLC46A1) is a proton-folate symporter that is abundantly expressed in solid tumors and normal tissues, such as duodenum...
  6. pmc Identification of a functionally critical GXXG motif and its relationship to the folate binding site of the proton-coupled folate transporter (PCFT-SLC46A1)
    Rongbao Zhao
    Depts of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Am J Physiol Cell Physiol 303:C673-81. 2012
    The proton-coupled folate transporter (PCFT) mediates intestinal folate absorption, and loss-of-function mutations in this gene result in the autosomal recessive disorder hereditary folate malabsorption...
  7. pmc The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption
    Rongbao Zhao
    Departments of Medicine, Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood 110:1147-52. 2007
    ..studies in 1 family revealed that the molecular basis for this disorder is a loss-of-function mutation in the PCFT gene encoding a proton-coupled folate transporter...
  8. pmc A P425R mutation of the proton-coupled folate transporter causing hereditary folate malabsorption produces a highly selective alteration in folate binding
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 302:C1405-12. 2012
    Proton-coupled folate transporter (PCFT) mediates folate intestinal absorption and transport across the choroid plexus, processes defective in subjects with hereditary folate malabsorption (HFM)...
  9. doi Functional elements in the minimal promoter of the human proton-coupled folate transporter
    Michal Stark
    Dept of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Biochem Biophys Res Commun 388:79-85. 2009
    The proton-coupled folate transporter (PCFT) is the dominant intestinal folate transporter, however, its promoter has yet to be revealed. Hence, we here cloned a 3...
  10. pmc Role of the glutamate 185 residue in proton translocation mediated by the proton-coupled folate transporter SLC46A1
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 297:C66-74. 2009
    The proton-coupled folate transporter (PCFT) SLC46A1 mediates uphill folate transport into enterocytes in proximal small intestine coupled to the inwardly directed proton gradient...
  11. pmc Identification of novel mutations in the proton-coupled folate transporter (PCFT-SLC46A1) associated with hereditary folate malabsorption
    Daniel Sanghoon Shin
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Genet Metab 103:33-7. 2011
    ..disorder, recently shown to be due to loss-of-function mutations of the proton-coupled folate transporter (PCFT-SLC46A1), resulting in systemic and central nervous system folate deficiency...
  12. pmc A novel PCFT gene mutation (p.Cys66LeufsX99) causing hereditary folate malabsorption
    Esther Meyer
    Department of Medical and Molecular Genetics, Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT, UK
    Mol Genet Metab 99:325-8. 2010
    ..Mutations in the intestinal folate transporter PCFT have been reported previously in only 10 individuals with this disorder...
  13. pmc Synthesis and biological activity of a novel series of 6-substituted thieno[2,3-d]pyrimidine antifolate inhibitors of purine biosynthesis with selectivity for high affinity folate receptors over the reduced folate carrier and proton-coupled folate transpo
    Yijun Deng
    Graduate Program in Cancer Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Med Chem 52:2940-51. 2009
    ..ovary (CHO) cells that expressed FRs but not the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT). However, the compounds were inactive toward CHO cells that lacked FRs but contained either the RFC or PCFT...
  14. doi PCFT/SLC46A1 promoter methylation and restoration of gene expression in human leukemia cells
    Nitzan Gonen
    The Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biochem Biophys Res Commun 376:787-92. 2008
    The proton-coupled folate transporter (PCFT/SLC46A1) displays optimal and prominent folate and antifolate transport activity at acidic pH in human carcinoma cells but poor activity in leukemia cells...
  15. pmc Membrane topological analysis of the proton-coupled folate transporter (PCFT-SLC46A1) by the substituted cysteine accessibility method
    Rongbao Zhao
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 49:2925-31. 2010
    The proton-coupled folate transporter (PCFT) mediates intestinal folate absorption. Loss-of-function mutations in this gene are the molecular basis for the autosomal recessive disorder, hereditary folate malabsorption...
  16. pmc Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption
    Kris Mahadeo
    Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 299:C1153-61. 2010
    The proton-coupled folate transporter (PCFT-SLC46A1) is required for intestinal folate absorption and is mutated in the autosomal recessive disorder, hereditary folate malabsorption (HFM)...
  17. pmc The obligatory intestinal folate transporter PCFT (SLC46A1) is regulated by nuclear respiratory factor 1
    Nitzan Gonen
    Fred Wyszkowski Cancer Research Laboratory, Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 285:33602-13. 2010
    ..The proton-coupled folate transporter (PCFT/SLC46A1) mediates obligatory intestinal folate absorption...
  18. pmc Random mutagenesis of the proton-coupled folate transporter (SLC46A1), clustering of mutations, and the bases for associated losses of function
    Rongbao Zhao
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 286:24150-8. 2011
    Loss-of-function mutations in the proton-coupled folate transporter (PCFT, SLC46A1) result in the autosomal recessive disorder, hereditary folate malabsorption (HFM)...
  19. doi Mutation of the proton-coupled folate transporter gene (PCFT-SLC46A1) in Turkish siblings with hereditary folate malabsorption
    Berna Atabay
    Clinics of Pediatrics, Tepecik Training and Research Hospital, Izmir, Turkey
    Pediatr Hematol Oncol 27:614-9. 2010
    ..204_205 delCC) within the first exon of the proton-coupled folate transporter (PCFT) gene, causing a frameshift. Low blood and cerebrospinal fluid folate levels were detected at ages 3.5 and 1 month...
  20. doi The human proton-coupled folate transporter (hPCFT): modulation of intestinal expression and function by drugs
    Bradley L Urquhart
    Division of Clinical Pharmacology, Department of Medicine, University of Western Ontario, London Health Sciences Centre Univ Campus, 339 Windermere Rd, London, Ontario, Canada
    Am J Physiol Gastrointest Liver Physiol 298:G248-54. 2010
    ..Importantly, we note that PPI drug use appears to be associated with reduced hPCFT expression in vivo...
  21. ncbi Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter
    Yasuhiro Nakai
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    J Pharmacol Exp Ther 322:469-76. 2007
    The functional characteristics of human proton coupled folate transporter (hPCFT)/heme carrier protein (HCP) 1 were investigated...
  22. ncbi Haem carrier protein 1 (HCP1): Expression and functional studies in cultured cells
    Gladys O Latunde-Dada
    Department of Biochemistry, School of Biomedical and Health Sciences, Franklin Wilkins Building, King s College London, 150 Stamford Street, London, SE1 9NH, United Kingdom
    FEBS Lett 580:6865-70. 2006
    ..The current studies describe the expression and functional analysis of HCP1 in cultured Caco-2 cells, a commonly used model of human intestinal cells...
  23. ncbi Apical membrane targeting and trafficking of the human proton-coupled transporter in polarized epithelia
    Veedamali S Subramanian
    Departments of Medicine, University of California, Irvine, California, USA
    Am J Physiol Cell Physiol 294:C233-40. 2008
    ..For the first time, our data present information regarding structure-function and membrane targeting of the hPCFT polypeptide, as well as the mechanisms that control its steady-state expression in polarized cells...
  24. pmc The functional roles of the His247 and His281 residues in folate and proton translocation mediated by the human proton-coupled folate transporter SLC46A1
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Biol Chem 284:17846-57. 2009
    This report addresses the functional role of His residues in the proton-coupled folate transporter (PCFT; SLC46A1), which mediates intestinal folate absorption. Of ten His residues, only H247A and H281A mutations altered function...
  25. pmc The clinical course and genetic defect in the PCFT gene in a 27-year-old woman with hereditary folate malabsorption
    Sang Hee Min
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA
    J Pediatr 153:435-7. 2008
    We report 2 sequential homozygous mutations in the recently cloned proton-coupled folate transporter (PCFT) gene, resulting in the absence of this protein, in a 27-year-old woman with hereditary folate malabsorption, normal in all ..
  26. pmc Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda
    Chacko Jobichen
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e12910. 2010
    ..EvpC is one of the three major EVP secreted proteins and shares high sequence similarity with Hcp1, a key T6SS virulence factor from Pseudomonas aeruginosa. EvpC contributes to the virulence of E...
  27. doi A genomic island of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough promotes survival under stress conditions while decreasing the efficiency of anaerobic growth
    Shawna Johnston
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada
    Environ Microbiol 11:981-91. 2009
    ..recombinases and transposases, rubredoxin:oxygen oxidoreductase-1 (Roo1) and hybrid cluster protein-1 (Hcp1), which promote survival in air and nitrite stress...
  28. ncbi Quantitation of toxic arsenic species and arsenobetaine in Pacific oysters using an off-line process with hydride generation-atomic absorption spectroscopy
    Tung Ming Hsiung
    Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, Republic of China
    J Agric Food Chem 54:2470-8. 2006
    ..were determined with a sensitive hydride generation-packed coldfinger trap-atomic absorption spectrometric (HG-PCFT-AAS) coupled system...
  29. doi Characterization of uptake of folates by rat and human blood-brain barrier endothelial cells
    Joao R Araujo
    Department of Biochemistry U38 FCT, Faculty of Medicine, University of Porto, Porto, Portugal
    Biofactors 36:201-9. 2010
    ..expression of reduced folate transporter (RFC), but neither of FRalpha nor of proton-coupled folate transporter (PCFT) in RBE4 cells, and mRNA expression of RFC and PCFT, but not of FRalpha, in hCMEC/D3 cells...
  30. pmc H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine
    David T Thwaites
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Exp Physiol 92:603-19. 2007
    ..OATP2B1 (SLC02B1); the monocarboxylate transporter MCT1 (SLC16A1); the proton-coupled folate transporter PCFT (SLC46A1); the sodium-glucose linked cotransporter SGLT1 (SLC5A1); and the excitatory amino acid carrier EAAC1 (SLC1A1)...
  31. doi Expression of folate transporters in human placenta and implications for homocysteine metabolism
    N Solanky
    School of Clinical and Laboratory Sciences, University of Manchester, Manchester Academic Health Science Centre, St Mary s Hospital, Manchester, UK
    Placenta 31:134-43. 2010
    ..These data support the concept that placental folate transport is established early in pregnancy, providing folate for utilisation in placental Hcy metabolism...
  32. pmc Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types
    Nagavedi S Umapathy
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, USA
    Invest Ophthalmol Vis Sci 48:5299-305. 2007
    ..Recently, a new proton-coupled folate transporter (PCFT) in human intestine was reported...
  33. pmc Molecular and biochemical characterization of folate transport proteins in retinal Müller cells
    B Renee Bozard
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912 2000, USA
    Invest Ophthalmol Vis Sci 51:3226-35. 2010
    ..To analyze the mechanisms of folate uptake in retinal Müller cells...
  34. pmc The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei
    Mary N Burtnick
    Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama 36688, USA
    Infect Immun 79:1512-25. 2011
    ..Recombinant Hcp2 protected 80% of mice against a lethal challenge with K96243, while recombinant Hcp1, Hcp3, and Hcp6 protected 50% of mice against challenge. Hcp6 was the only Hcp constitutively produced by B...
  35. ncbi Characterization of a novel barley protein, HCP1, that interacts with the Brome mosaic virus coat protein
    Yasushi Okinaka
    Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Mol Plant Microbe Interact 16:352-9. 2003
    ..One of the candidate cDNAs encodes a unique oxidoreductase enzyme, designated HCP1. HCP1 was found predominantly in the soluble fractions after differential centrifugation of BMV-infected and mock-..
  36. doi Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model
    Katsuhisa Inoue
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho ku, Nagoya 467 8603, Japan
    Am J Physiol Gastrointest Liver Physiol 294:G660-8. 2008
    Proton-coupled folate transporter/heme carrier protein 1 (PCFT/HCP1) has recently been identified as a transporter that mediates the translocation of folates across the cellular membrane by a proton-coupled mechanism and suggested to be ..
  37. ncbi Fifteen-year follow-up of 92 hospitalized adults with Down's syndrome: incidence of cognitive decline, its relationship to age and neuropathology
    M L Margallo-Lana
    Prudhoe Hospital, Northumberland, UK
    J Intellect Disabil Res 51:463-77. 2007
    ..The clinical and neuropathological features associated with dementia in Down's syndrome (DS) are not well established. Aims To examine clinico-pathological correlations and the incidence of cognitive decline in a cohort of adults with DS...
  38. doi Effect of cannabinoids upon the uptake of folic acid by BeWo cells
    Joao R Araujo
    Department of Biochemistry U38 FCT, Faculty of Medicine, University of Porto, Porto, Portugal
    Pharmacology 83:170-6. 2009
    ..The acute effects of cannabinoid receptor agonists do not seem to be mediated by the cannabinoid receptor, and with a few exceptions the chronic effects do not seem to be related to changes in the expression levels of FA transporters...
  39. ncbi Involvement of reduced folate carrier 1 in the inner blood-retinal barrier transport of methyltetrahydrofolate
    Ken ichi Hosoya
    Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Japan
    Drug Metab Pharmacokinet 23:285-92. 2008
    ..Although both RFC1 and proton-coupled folate transporter (PCFT) mRNA, which are pH-sensitive folate transporters, are expressed in TR-iBRB2 cells and isolated rat retinal ..
  40. ncbi Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction
    Andong Qiu
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 293:C1669-78. 2007
    ..a human gene that encodes a novel folate transporter [Homo sapiens proton-coupled folate transporter (HsPCFT); SLC46A1] required for intestinal folate absorption...
  41. doi Proton-coupled folate transporter (PCFT): molecular cloning, tissue expression patterns and the effects of dietary folate supplementation on mRNA expression in laying hens
    M Jing
    Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada
    Br Poult Sci 51:635-8. 2010
    ..and effects of dietary folate supplementation on mRNA concentrations of the proton-coupled folate transporter (PCFT) in chicken. 2...
  42. doi Reduced levels of folate transporters (PCFT and RFC) in membrane lipid rafts result in colonic folate malabsorption in chronic alcoholism
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India
    J Cell Physiol 226:579-87. 2011
    ..transport activity at the CAM was associated with down-regulation of the proton-coupled folate transporter (PCFT) and the reduced folate carrier (RFC) which resulted in decreased PCFT and RFC protein levels in the colon of rats ..
  43. ncbi Isolation and characterization of a previously undetected human cAMP phosphodiesterase by complementation of cAMP phosphodiesterase-deficient Saccharomyces cerevisiae
    T Michaeli
    Cold Spring Harbor Laboratory, New York 11724
    J Biol Chem 268:12925-32. 1993
    ..Two of these genes are closely related to the Drosophila dunce cAMP-specific PDE. The third gene, which we named HCP1, encoded a novel cAMP-specific PDE...
  44. ncbi Primary carcinoma of the fallopian tube and epithelial ovarian carcinoma: a case-control analysis
    Melissa S Dunn
    Division of Gynecologic Oncology, Holden Comprehensive Cancer Center, University of Iowa, Iowa City, USA
    J Reprod Med 53:691-4. 2008
    To determine whether differences exist in clinicopathologic variables or survival between women with primary carcinoma of the fallopian tube (PCFT) and with epithelial ovarian carcinoma (EOC).
  45. pmc N-linked glycosylation and its impact on the electrophoretic mobility and function of the human proton-coupled folate transporter (HsPCFT)
    Ersin Selcuk Unal
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochim Biophys Acta 1778:1407-14. 2008
    The human proton-coupled folate transporter (HsPCFT, SLC46A1) mediates intestinal absorption of folates and transport of folates into the liver, brain and other tissues...
  46. doi Molecular and functional characteristics of proton-coupled folate transporter
    Hiroaki Yuasa
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    J Pharm Sci 98:1608-16. 2009
    Proton-coupled folate transporter (PCFT) has recently been identified as the molecular entity of the carrier-mediated intestinal folate transport system...
  47. pmc The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system
    Lisa G Pell
    Department of Biochemistry, University of Toronto, Medical Sciences Building, Toronto, ON M5S 1A8, Canada
    Proc Natl Acad Sci U S A 106:4160-5. 2009
    ..A remarkable structural similarity is also seen to Hcp1, a component of the bacterial type VI secretion system...
  48. ncbi Identification of proton-coupled high-affinity human intestinal folate transporter mutated in human hereditary familial folate malabsorption
    George Wolf
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California, USA
    Nutr Rev 65:554-7. 2007
    ..The transporter, named PCFT/HCP1, is a protein of approximately 50 kDa and functions as a reversible, electrogenic, proton-coupled high-affinity ..
  49. pmc In vitro self-assembly of tailorable nanotubes from a simple protein building block
    Edward R Ballister
    Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:3733-8. 2008
    ..for generating discretely structured protein nanotubes from the simple ring-shaped building block, homohexameric Hcp1 from Pseudomonas aeruginosa...
  50. doi Comparison of short and long versions of the Prudhoe Cognitive Function Test and the K-BIT in participants with intellectual impairment
    Stephen P Tyrer
    Department of Psychiatry, Newcastle University, Royal Victoria Infirmary, Leazes Wing, Newcastle upon Tyne, UK
    J Autism Dev Disord 40:1000-5. 2010
    The Prudhoe Cognitive Function Test (PCFT) directly measures the cognitive abilities of people with intellectual impairment...
  51. pmc Hexamers of the type II secretion ATPase GspE from Vibrio cholerae with increased ATPase activity
    Connie Lu
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Structure 21:1707-17. 2013
    ..Here, four hexamers of Vibrio cholerae GspE(EpsE) are obtained when fused to Hcp1 as an assistant hexamer, as shown with native mass spectrometry...
  52. pmc Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus
    Lizhi Ma
    Department of Emergency Medicine, General Hospital of Chinese People s Armed Police Forces, Beijing 100039, China
    ScientificWorldJournal 2012:982140. 2012
    ..This work disclosed that the master quorum sensing (QS) regulator OpaR repressed the transcription of hcp1 encoding the structural component Hcp1 of T6SS1 in V...
  53. pmc Decreased activity of folate transporters in lipid rafts resulted in reduced hepatic folate uptake in chronic alcoholism in rats
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160 012, India
    Genes Nutr 8:209-19. 2013
    ..BLM) suggested it to be a carrier mediated and acidic pH dependent, with the major involvement of proton coupled folate transporter and folate binding protein in the uptake...
  54. pmc Mechanism of intestinal folate transport during folate deficiency in rodent model
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India
    Indian J Med Res 136:758-65. 2012
    ..However, much less is known about regulation. In this study we evaluated the mechanistic insights of folate absorption in an in vivo model of folate deficiency...
  55. ncbi Preliminary evaluation of a scale to assess cognitive function in adults with Down's syndrome: the Prudhoe Cognitive Function Test
    D W K Kay
    Department of Psychiatry, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
    J Intellect Disabil Res 47:155-68. 2003
    ..The aim of the present study was to evaluate the performance of non-demented adults with DS on a subject-directed instrument, the Prudhoe Cognitive Function Test (PCFT), preliminary to its serial use in a prospective study.
  56. doi Mechanistic insights of intestinal absorption and renal conservation of folate in chronic alcoholism
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India
    Alcohol 47:121-30. 2013
    ..The folate transporters PCFT and RFC were found to be associated with lipid rafts of membrane surfaces in intestine and kidney...
  57. pmc Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2
    Dor Salomon
    Department of Molecular Biology, University of Texas Southwestern, Medical Center, Dallas, Texas, United States of America
    PLoS ONE 8:e61086. 2013
    ..We monitored the expression and secretion of the signature T6SS secreted proteins Hcp1 and Hcp2, and found that both T6SSs are differentially regulated by quorum sensing and surface sensing...
  58. doi Folate malabsorption is associated with down-regulation of folate transporter expression and function at colon basolateral membrane in rats
    Nissar Ahmad Wani
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India
    Br J Nutr 107:800-8. 2012
    ..The decreased uptake was associated with down-regulation of proton-coupled folate transporter (PCFT) and RFC expression at mRNA and protein levels...
  59. doi Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development
    J Cherukad
    Centre for Trophoblast Research, Dept of Physiology, Development and Neuroscience, University of Cambridge, Physiological Laboratories, Downing Street, Cambridge CB2 3EG, UK
    Placenta 33:440-8. 2012
    ..protein expression analysis of folate receptor (Folr), folate transporters (proton-coupled folate receptor [Slc46a1 or PCFT] and reduced folate carrier-1 [Rfc1]) and folate metabolic enzymes (5,10-methylenetetrahydrofolate ..
  60. ncbi Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers
    Ying Yu
    Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Institute of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China
    Arch Microbiol 194:827-35. 2012
    ..However, icmF2 is contained in all strains of both clinical and environmental sources. Deletion of either icmF1 or hcp1 significantly reduced bacterial adhesion to Caco-2 cells or HeLa monolayers...
  61. pmc Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts
    Minna Haapalainen
    Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
    J Bacteriol 194:4810-22. 2012
    ..Two copies of hcp genes are present in the P. syringae pv. tomato DC3000 genome, hcp1 (PSPTO_2539) and hcp2 (PSPTO_5435)...
  62. ncbi Stereoselective transport of amethopterin enantiomers by the proton-coupled folate transporter
    Tomoya Narawa
    School of Pharmacy, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo, Japan
    Drug Metab Pharmacokinet 25:283-9. 2010
    ..L-amethopterin, L-MTX) and its antipode (D-amethopterin, D-MTX) by the proton-coupled folate transporter (PCFT) was examined using PCFT-expressing HEK293 cells (PCFT-HEK293 cells)...
  63. ncbi Study on absorption sites of quinidine and methotrexate in rat intestine
    T Murakami
    Laboratory of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Hiroshima International University, Japan
    Pharmazie 65:440-7. 2010
    ..P-gp) and methotrexate (a substrate for multiple-transporters including proton-coupled folate transporter, PCFT) were examined to find their main absorption sites along the intestine employing rats...
  64. pmc Synthesis, biological, and antitumor activity of a highly potent 6-substituted pyrrolo[2,3-d]pyrimidine thienoyl antifolate inhibitor with proton-coupled folate transporter and folate receptor selectivity over the reduced folate carrier that inhibits β-gl
    Lei Wang
    Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh 15219, Pennsylvania 15282, United States
    J Med Chem 54:7150-64. 2011
    ..the proliferation of cells expressing folate receptors (FRs) α or β, or the proton-coupled folate transporter (PCFT), including KB and IGROV1 human tumor cells, much more potently than 4...
  65. doi Prevalence of a loss-of-function mutation in the proton-coupled folate transporter gene (PCFT-SLC46A1) causing hereditary folate malabsorption in Puerto Rico
    Kris M Mahadeo
    Division of Pediatric Hematology Oncology, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Pediatr 159:623-7.e1. 2011
    To determine whether subjects of Puerto Rican heritage are at increased risk for a specific mutation of the proton-coupled folate transporter (PCFT) causing hereditary folate malabsorption (HFM).
  66. ncbi The molecular identity and characterization of a Proton-coupled Folate Transporter--PCFT; biological ramifications and impact on the activity of pemetrexed
    Rongbao Zhao
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cancer Metastasis Rev 26:129-39. 2007
    ..activity, (2) its relationship to, and properties of, the recently identified Proton-Coupled Folate Transporter (PCFT), (3) the physiological and pharmacological roles of this transporter, particularly with respect to pemetrexed, and ..
  67. pmc Regulation of type VI secretion system during Burkholderia pseudomallei infection
    Yahua Chen
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597
    Infect Immun 79:3064-73. 2011
    ..VirAG and BprC initiate different transcriptional start sites within T6SS-1, and VirAG is able to activate the hcp1 promoter directly...
  68. pmc Cation exchange HPLC analysis of desmosines in elastin hydrolysates
    Joanna Perła-Kaján
    Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 60 637 Poznan, Poland
    Anal Bioanal Chem 401:2473-9. 2011
    ..We also found that desmosines levels were lower in lung elastin isolated from hyperhomocysteinemic Pcft(-/-) mice deficient in intestinal folate transport compared with wild-type Pcft(+/+) animals.
  69. pmc Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins
    Abderrahman Hachani
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 286:12317-27. 2011
    ..We demonstrate that Hcp1 secretion requires either VgrG1a or VgrG1c, which may act independently to puncture the bacterial envelope and give ..
  70. pmc Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa
    Jerzy Osipiuk
    Argonne National Laboratory, Biosciences Division, Midwest Center for Structural Genomics and Structural Biology Center, IL 60439, USA
    J Struct Funct Genomics 12:21-6. 2011
    ..locus encodes for several proteins that has been proposed to participate in protein transport including the Hcp1 protein, which forms hexameric rings that assemble into nanotubes in vitro...
  71. pmc Polarized distribution of heme transporters in retinal pigment epithelium and their regulation in the iron-overload disease hemochromatosis
    Jaya P Gnana-Prakasam
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Health Sciences University, Augusta, Georgia 30912, USA
    Invest Ophthalmol Vis Sci 52:9279-86. 2011
    FLVCR, BCRP, and PCFT/HCP-1 represent the three heme transporters identified thus far in mammalian cells, but there is very little known about their expression and regulation in the retina...
  72. ncbi Does hepatic hepcidin play an important role in exercise-associated anemia in rats?
    Yu Qian Liu
    College of Physical Education, Hebei Normal University, Shijiazhuang, China
    Int J Sport Nutr Exerc Metab 21:19-26. 2011
    ..The protein levels of divalent metal transporter 1 (DMT1), ferroportin1 (FPN1), and heme-carrier protein 1 (HCP1) of duodenum epithelium were examined by Western blot...
  73. pmc Iron homeostasis and nutritional iron deficiency
    Elizabeth C Theil
    Council on BioIron at Children s Hospital Oakland Research Institute, Department of Nutrition Sciences and Toxicology, University of California, Berkeley, CA 94609, USA
    J Nutr 141:724S-728S. 2011
    ..Iron absorption depends on membrane transporter proteins DMT1, PCP/HCP1, ferroportin (FPN), TRF2, and matriptase 2...
  74. doi Interactions between genetic variants of folate metabolism genes and lifestyle affect plasma homocysteine concentrations in the Boston Puerto Rican population
    Tao Huang
    Department of Food Science and Nutrition, Zhejiang University, Hangzhou, People s Republic of China
    Public Health Nutr 14:1805-12. 2011
    ..To investigate genetic and lifestyle factors and their interactions on plasma homocysteine (Hcy) concentrations in the Boston Puerto Rican population...
  75. doi Hijacking solute carriers for proton-coupled drug transport
    Catriona M H Anderson
    Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom
    Physiology (Bethesda) 25:364-77. 2010
    ..These transporters include PepT1 and PepT2 (SLC15A1/2), PCFT (SLC46A1), PAT1 (SLC36A1), OAT10 (SLC22A13), OATP2B1 (SLCO2B1), MCT1 (SLC16A1), and MATE1 and MATE2-K (SLC47A1/2).
  76. pmc Targeting the proton-coupled folate transporter for selective delivery of 6-substituted pyrrolo[2,3-d]pyrimidine antifolate inhibitors of de novo purine biosynthesis in the chemotherapy of solid tumors
    Sita Kugel Desmoulin
    Graduate Program in Cancer Biology, Wayne State University School of Medicine, Detroit, Michigan, USA
    Mol Pharmacol 78:577-87. 2010
    The proton-coupled folate transporter (PCFT) is a folate-proton symporter with an acidic pH optimum, approximating the microenvironments of solid tumors...
  77. doi The effect of folate status on the uptake of physiologically relevant compounds by Caco-2 cells
    Sandra Tavares
    Department of Biochemistry U38 FCT, Faculty of Medicine, University of Porto, Porto, Portugal
    Eur J Pharmacol 640:29-37. 2010
    ..is not changed; and (4) the steady-state mRNA levels of the folic acid transporters RFC (reduced folate carrier), PCFT (proton-coupled folate transporter) and FRalpha (folate receptor alpha), of the organic cation transporter OCT1 (..
  78. doi Haem and folate transport by proton-coupled folate transporter/haem carrier protein 1 (SLC46A1)
    Abas H Laftah
    Division of Nutritional Sciences, School of Biomedical and Health Sciences, King s College London, Franklin Wilkins Building, London SE1 9NH, UK
    Br J Nutr 101:1150-6. 2009
    Haem carrier protein 1 (HCP1) was originally identified and characterised as a mammalian haem transporter...
  79. ncbi Placental folate transport during pregnancy
    Satoru Yasuda
    Laboratory of Clinical Pharmaceutics and Therapeutics, Division of Pharmascience, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 72:2277-84. 2008
    ..TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta...
  80. doi A novel loss-of-function mutation in the proton-coupled folate transporter from a patient with hereditary folate malabsorption reveals that Arg 113 is crucial for function
    Inbal Lasry
    The Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Blood 112:2055-61. 2008
    ..HFM) patients harbor inactivating mutations including R113S in the proton-coupled folate transporter (PCFT), an intestinal folate transporter with optimal activity at acidic pH...
  81. pmc The proton-coupled folate transporter: impact on pemetrexed transport and on antifolates activities compared with the reduced folate carrier
    Rongbao Zhao
    Departments of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Pharmacol 74:854-62. 2008
    The reduced folate carrier (RFC) and the proton-coupled folate transporter (PCFT) are ubiquitously expressed in normal and malignant mammalian tissues and in human solid tumor cell lines...
  82. ncbi The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport
    Satoru Yasuda
    Laboratory of Clinical Pharmaceutics and Therapeutics, Division of Pharmascience, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Biosci Biotechnol Biochem 72:329-34. 2008
    ..levels of the mRNA of the folate receptor alpha (FRalpha), the reduced folate carrier (RFC), and heme carrier protein 1 (HCP1) were determined in BeWo cells by RT-PCR analysis...
  83. ncbi Chromosome localizations of genes for five cAMP-specific phosphodiesterases in man and mouse
    A Milatovich
    Department of Genetics, Stanford University Medical Center, California 94305
    Somat Cell Mol Genet 20:75-86. 1994
    ..The high-affinity cAMP-specific phosphodiesterase gene (HCP1) was mapped to human chromosome 8q13-q22...
  84. ncbi Characterization of folate uptake by choroid plexus epithelial cells in a rat primary culture model
    Jan B Wollack
    Department of Pediatrics, Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA
    J Neurochem 104:1494-503. 2008
    ..The proton-coupled folate transporter, proton-coupled folate transporter (PCFT), mRNA was also shown to be expressed in choroid plexus epithelial cells...
  85. ncbi Serous fallopian tube carcinoma: a retrospective, multi-institutional case-control comparison to serous adenocarcinoma of the ovary
    Kathleen N Moore
    University of Oklahoma, Division of Gynecologic Oncology, 920 SL Young Blvd, Oklahoma City, OK 73104, USA
    Gynecol Oncol 107:398-403. 2007
    Primary carcinoma of the fallopian tube (PCFT) is a rare malignancy comprising 1% of genital tract cancers. We sought to compare survival trends between PCFT and ovarian carcinoma (OC) patients (pts) in a matched, case-control comparison.
  86. ncbi Heme carrier protein (HCP-1) spatially interacts with the CD163 hemoglobin uptake pathway and is a target of inflammatory macrophage activation
    Christian A Schaer
    Department of Medicine, University Hospital, CH 8091 Zurich, Switzerland
    J Leukoc Biol 83:325-33. 2008
    ..In this study, we show that the heme transporter heme carrier protein 1 (HCP-1) is expressed in human macrophages...
  87. ncbi Effect of folate oversupplementation on folate uptake by human intestinal and renal epithelial cells
    Balasubramaniem Ashokkumar
    Department of Medicine, Division of Nephrology, and the Department of Physiology and Biophysics, University of California, Irvine, CA, USA
    Am J Clin Nutr 86:159-66. 2007
    ..Hence, food fortification and intake of supplemental folate are widespread...
  88. ncbi The role of transporters in cellular heme and porphyrin homeostasis
    Partha Krishnamurthy
    Department of Pharmaceutical Sciences, MS 313, St Jude Children s Research Hospital, 332 N Lauderdale Avenue, Memphis, TN 38105, USA
    Pharmacol Ther 114:345-58. 2007
    ..In this review, we focus on the roles of the recently identified heme/porphyrin transport proteins heme carrier protein 1 (HCP1), FLVCR, Abcg2 and Abcb6 and discuss how these transporters contribute to intracellular heme and ..
  89. ncbi Pemetrexed: biochemical and cellular pharmacology, mechanisms, and clinical applications
    Shrikanta Chattopadhyay
    Departments of Medicine and Molecular Pharmacology, The Albert Einstein College of Medicine Cancer Center, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cancer Ther 6:404-17. 2007
    ..carrier for which pemetrexed has high affinity, recently identified as the proton-coupled folate transporter (PCFT)...
  90. ncbi Iron uptake and metabolism in the new millennium
    Louise L Dunn
    Iron Metabolism and Chelation Program, Department of Pathology, Blackburn Building D06, University of Sydney, Sydney, NSW 2006, Australia
    Trends Cell Biol 17:93-100. 2007
    ..Here, we focus on these intriguing new molecules and the insights gained from them into cellular iron uptake and the regulation of iron metabolism...
  91. ncbi Effect of lipopolysaccharide and bleeding on the expression of intestinal proteins involved in iron and haem transport
    J Krijt
    Institute of Pathophysiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 52:1-5. 2006
    ..Using quantitative real-time PCR, we examined the expression of Hcp1 and other intestinal iron-transporting proteins in male C57BL/6 mice with experimentally altered iron homeostasis...
  92. ncbi Alternative splicing of the high affinity cAMP-specific phosphodiesterase (PDE7A) mRNA in human skeletal muscle and heart
    P Han
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 272:16152-7. 1997
    ..the isozyme expressed in human skeletal muscle and the protein product of the previously isolated isozyme HCP1 (designated HSPDE7A1)...
  93. doi Molecular mechanisms of adaptation to folate deficiency
    Ilan Ifergan
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Vitam Horm 79:99-143. 2008
    ..including the reduced folate carrier (RFC), folate receptor (FR) as well as the proton-coupled folate transporter (PCFT), a recently identified intestinal folate influx transporter optimally functioning at the acidic microclimate of ..
  94. ncbi Structural and functional conservation of human and yeast HCP1 genes which can suppress the growth defect of the Saccharomyces cerevisiae ire15 mutant
    J Nikawa
    Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
    Gene 171:107-11. 1996
    ..Here, we isolated a new human cDNA, HCP1, which suppresses the ire15 mutation by genetic complementation...
  95. pmc Mechanism and regulation of folate uptake by pancreatic acinar cells: effect of chronic alcohol consumption
    Hamid M Said
    VA Medical Center 151, Long Beach, CA 90822, USA
    Am J Physiol Gastrointest Liver Physiol 298:G985-93. 2010
    ..Both the reduced folate carrier (RFC) and the proton-coupled folate transporter (PCFT) were found to be expressed in AR42J and in primary pancreatic acinar cells, as well as in native human pancreas ..
  96. ncbi Beam propagation factor of partially coherent flat-topped beams in a turbulent atmosphere
    Youquan Dan
    College of Electronics Information, Sichuan University, Chengdu 610064, Sichuan, China
    Opt Express 16:15563-75. 2008
    ..function (WDF) has been used to study the beam propagation factor (M(2)-factor) for partially coherent flat-topped (PCFT) beams with circular symmetry in a turbulent atmosphere...
  97. pmc Synthesis and antitumor activity of a novel series of 6-substituted pyrrolo[2,3-d]pyrimidine thienoyl antifolate inhibitors of purine biosynthesis with selectivity for high affinity folate receptors and the proton-coupled folate transporter over the reduc
    Lei Wang
    Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, Pennsylvania 15282, USA
    J Med Chem 53:1306-18. 2010
    ..1-3) were synthesized as substrates for folate receptors (FRs) and the proton-coupled folate transporter (PCFT)...
  98. pmc The role of folate receptor alpha (FRalpha) in the response of malignant pleural mesothelioma to pemetrexed-containing chemotherapy
    J E Nutt
    Northern Institute for Cancer Research, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Br J Cancer 102:553-60. 2010
    ..This study investigates the response to pemetrexed in a panel of eight mesothelioma cell lines and the clinical outcome for patients treated with pemetrexed in relation to folate receptor alpha (FRalpha)...
  99. ncbi Greeen tea extracts lower serum folates in rats at very high dietary concentrations only and do not affect plasma folates in a human pilot study
    K Augustin
    Institute of Human Nutrition and Food Science, Christian Albrechts University, Kiel, Germany
    J Physiol Pharmacol 60:103-8. 2009
    ..in rats, while mRNA concentrations of reduced folate carrier, proton-coupled folate transporter/heme carrier protein 1, and dihydrofolate reductase (DHFR) remained unchanged in intestinal mucosa...
  100. pmc Reversible severe combined immunodeficiency phenotype secondary to a mutation of the proton-coupled folate transporter
    Arturo Borzutzky
    Division of Immunology, Children s Hospital Boston, Boston, MA, USA
    Clin Immunol 133:287-94. 2009
    ..malabsorption is a rare inborn error of metabolism due to mutations in the proton-coupled folate transporter (PCFT). Clinical presentation of PCFT deficiency may mimic severe combined immune deficiency (SCID)...

Research Grants85

  1. Proton-coupled folate/antifolate transport
    ISRAEL DAVID GOLDMAN; Fiscal Year: 2010
    ..This was accomplished with the identification of the "Proton-Coupled Folate Transporter" (PCFT)...
  2. Novel Type IV Pili of Enterohemorrhagic E. coli O157:H7
    Jorge Giron; Fiscal Year: 2004
    ..These pili, herein called hemorrhagic coli pili 1 (Hcp1), belong to the virulence-associated type IV pili family...
  3. MOLECULAR REGULATION OF FOLATE AND ANTIFOLATE TRANSPORT
    Larry H Matherly; Fiscal Year: 2010
    ....
  4. Intestinal Iron Transport in Iron Deficiency/Anemia
    James Collins; Fiscal Year: 2005
    ..abstract_text> ..
  5. DYNAMIC AND KINETIC BEHAVIOR OF FOLATE METABOLISM
    Andrew Clifford; Fiscal Year: 2001
    ..abstract_text> ..
  6. Molecular Mechanisms of Intestinal Metal Ion Transport During Iron-Deficiency
    James Collins; Fiscal Year: 2007
    ..Moreover, this proposed investigation is novel, as studies addressing the impact of increased enterocyte and liver copper levels during iron-deficiency have not been reported to date. ..
  7. Quantitation of Tocopherol Metabolism in Humans
    Andrew Clifford; Fiscal Year: 2009
    ..Particular attention will be directed to the absorption phase. The results are expected to provide information about vitamin E biopotency and eventually improved Dietary Recommendations. ..
  8. IN VIVO DYNAMIC OF BETA-CAROTENE METABOLISM IN HUMANS
    Andrew Clifford; Fiscal Year: 2006
    ....
  9. Quantitation of Tocopherol Metabolism in Humans
    ANDREW JOSEPH CLIFFORD; Fiscal Year: 2010
    ..Particular attention will be directed to the absorption phase. The results are expected to provide information about vitamin E biopotency and eventually improved Dietary Recommendations. ..
  10. DYNAMIC AND KINETIC BEHAVIOR OF FOLATE METABOLISM
    Andrew Clifford; Fiscal Year: 2005
    ..We will determine the in vivo kinetic behavior of a tracer dose of folate in blood, urine, and stool. We will then compare the behavior of folate metabolism (functional endpoint) among the various genotypes. ..
  11. IN VIVO DYNAMIC OF BETA-CAROTENE METABOLISM IN HUMANS
    Andrew Clifford; Fiscal Year: 2002
    ..These studies can serve as a basis for future investigations into the influence of environmental, genetic, and drug/nutrient interactions on the behavior of beta-carotene metabolism. ..
  12. Fe, O2, mRNA and Ferritin in Vitro & in Cells
    Elizabeth Theil; Fiscal Year: 2007
    ..abstract_text> ..
  13. MOLECULAR REGULATION OF FOLATE AND ANTIFOLATE TRANSPORT
    Larry Matherly; Fiscal Year: 2007
    ..abstract_text> ..
  14. MOLECULAR CORRELATES OF METHOTREXATE IN CHILDHOOD ALL
    Larry Matherly; Fiscal Year: 2007
    ..abstract_text> ..
  15. Human Intestinal Biotin Uptake:Molecular /Cell Mechanisms
    HAMID SAID; Fiscal Year: 2007
    ..This should ultimately assist us in the design of effective strategies to optimize biotin body homeostasis in conditions associated with biotin deficiency and sub-optimal levels. ..
  16. Intestinal Biotin Absorption: Physiological, Cellular and Molecular Aspects
    Hamid M Said; Fiscal Year: 2010
    ..Results of these investigations should help in the designing of effective strategies to optimize biotin body levels, especially in conditions associated with biotin deficiency and sub-optimal levels. 1 ..
  17. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ....
  18. Mechanisms of Pancreatic Thiamin Uptake:Effect of Alcohol
    Hamid M Said; Fiscal Year: 2010
    ....
  19. Human Intestinal Biotin Uptake: Molecular/Cell Mechanisms
    HAMID SAID; Fiscal Year: 2009
    ..This should ultimately assist us in the design of effective strategies to optimize biotin body homeostasis in conditions associated with biotin deficiency and sub-optimal levels. ..
  20. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    Elizabeth Theil; Fiscal Year: 2009
    ....
  21. Mechanisms of Pancreatic Thiamin Uptake:Effect of Alcohol
    HAMID SAID; Fiscal Year: 2009
    ....
  22. C elegans:a model to study intestinal water-soluble vitamins transport in vivo
    HAMID SAID; Fiscal Year: 2007
    ..This should be of great value in future investigations into the mechanisms and regulation of transport of water-soluble vitamins in the intestine (and other tissues) at the integrated whole animal level in vivo. ..
  23. TRAINING PROGRAM IN THE BIOLOGY OF CANCER
    Larry Matherly; Fiscal Year: 2007
    ..In accomplishing this, the training program has significantly contributed to meeting the critical national need for domestic and US-trained PhDs with a long term career commitment to cancer related research. ..
  24. Mechanism/regulation of human intestinal thiamin uptake
    HAMID SAID; Fiscal Year: 2007
    ..This should ultimately assist us in the designing of effective strategies to optimize thiamin body homeostasis in conditions associated with thiamin deficiency and sub-optimal levels. ..
  25. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ....
  26. INTESTINAL VITAMIN H UPTAKE--MOLECULAR/CELL REGULATION
    HAMID SAID; Fiscal Year: 2004
    ..These results should also contribute toward our understanding of regulation of SMVT function in other tissues that have been shown to express this transport system. ..
  27. FERRITIN AND IRON DEFICIENCY--A NEW LOOK AT THE PROBLEM
    Elizabeth Theil; Fiscal Year: 2000
    ....
  28. Workshop on BioIron in Thalassemia, Sickle Cell Disease and Hemochromatosis
    Elizabeth Theil; Fiscal Year: 2004
    ..Fuller understanding of iron homeostasis in Thalassemia, Sickle Cell Disease and Hemochromatosis and improved disease management strategies can be expected. ..
  29. MOLECULAR REGULATION OF FOLATE AND ANTIFOLATE TRANSPORT
    Larry Matherly; Fiscal Year: 2003
    ..abstract_text> ..
  30. Workshop on Biolron in Thalassemia & Sickle Cell Disease
    Elizabeth Theil; Fiscal Year: 2002
    ..abstract_text> ..
  31. IRON & OXYGEN BIOLOGY IN SCD, HH & BETA-THAL MICE
    Elizabeth Theil; Fiscal Year: 2002
    ..The resulting information will be used to help probe the efficiency of presently available and experimental chelators, not only in promoting iron excretion but also in the prevention of defined types of cellular damage. ..
  32. MOLECULAR CORRELATES OF METHOTREXATE IN CHILDHOOD ALL
    Larry Matherly; Fiscal Year: 2002
    ..The results of the proposed studies will provide a framework for further optimizing modern ALL, therapies including MTX and facilitate the development of approaches for circumventing resistance when it does arise. ..